Rh6AG445100

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015325
Physical Location & Seq
Reverse (-)
63126336 .. 63129575
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG445100.1

Sequence Viewer

Length: 1254 bp
ATGCCATCTAAGAGTGAGAATTCAGATAGAAACCATAGGATGGGGGTGACGGTAATATCTCCCCATTCATTGCCACAATATTTGCATTTACAGCAGTCGATACCAAACCAGAAAGCAGTAAAAACTAGTTGTTTGCTTAGCTATGGTAGACGAGGAGGAGTCTTGCTTGGTCAAAACAACCATCCAGACCCGACCACCGTTAATAGTATCACTACTGTTCCCGCCATGAAACTTAACTCATCATCATCTAGTATTGCAGCAGAGGCAACTAGTACTGGTGCCGCGGCCGCCGCCGACGATGTAGTGTTGGAATTGGAGCTGGGAGAGATTAAGGACAAGTACGTGAAGTGGAAGTGGAAGGGTCAGTACGCCATCAACTACATTGTTTCACATCCAGAAGATGCAAATCCGAATCCAAATGCTCCTCCTATTCTTCTTGTTCACGGCTTCGGCGCCTCCGTTCCTCACTGGCGCAGAAATATTAAGACATTGGCGAAGCATTATACAGTTTATGCTATTGACCTGATTGGCTTTGGTGCCTCAGATAAGCCGATAGGCTTTTCATACACCATGGAGAAATGGGCTGAGTTAATCTTGGATTTTTTGGATGAAGTTGTTCAGAAGCCTACTGTTTTAATAGGCAACTCTGTGGGAAGCCTTGCATGTGTAATCTCTGCCTCAGAATCTAAAAAGGGACTGGTTCGAGGGCTAGTGCTTTTGAATTGCTCTGGTGGGATGAATAACAAGGCCATTGTTGATGATTGGAGGATCAAGCTGCTCTTCCCTTTGCTCATGCTAATTGATTTCTTGTTGAAGCAACGACCTATTGCTTCCTTCATTTTTGAGCGTGTTAGACAGAGGGAAAGTTTAAGAAATATCTTATCATCTGTTTACGCCAACAAAGAATCTGTGGATGAAGAATTAGTAGAGATCATAATGGAACCAACAAGTGACCCAGGTGCACTGGACGCTTTTGTCTCGATCGTAACAGGCCCACCAGGACCAAACCCGGTCCAGTTAATGCCAGGCCTCTCCCTACCGATTCTAGTCCTATGGGGAGATGAAGACCCGTTCACTCCACTCGATGGGCCTGTTGGCAAATACTTCACTTCCTTACCTTCTCAGAAATCGAATGTGAGCCTCTCCATTTTACAAGGAGTTGGGCACTGCCCCCACGATGACAGGCCTAAGTTGGTCCACGACAAACTTCTTCCCTGGTTGGATAATCTTTCTATAGAAAATTCAGTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

417

Amino Acids

45.61

Weight (kDa)

6.27

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 142 - 396 2.1e-25 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 144 - 400 3.2e-21 Alpha/beta hydrolase family
Hydrolase_4 PF12146 144 - 394 3.2e-10 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38520 AT5G38520
fragaria_vesca FvH4_2g37190
malus_domestica MD08G1001000.v1.1
prunus_persica Prupe.1G353100_v2.0.a1
pyrus_communis pycom08g00040
rosa_chinensis RchiOBHm_Chr6g0304501
rosa_laevigata RLG00000010971
rosa_multiflora Rmu_sc0023263.1_g000001 Rmu_ssc0000218.1_g000058
rosa_roxburghii Rroxscaffold_7G00163690
rosa_rugosa Rorug06G0332600
rosa_samantha Rh6AG445100 Rh6CG457900 Rh6DG444700
rosa_wichuraiana Rw6G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 974
AccB1I GGYRCC 3 cut(s) 278, 452, 536
AccB7I CCANNNNNTGG 2 cut(s) 40, 1085
AccI GTMKAC 1 cut(s) 148
AccII CGCG 1 cut(s) 284
AciI CCGC 5 cut(s) 222, 282, 284, 288, 291
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 2 cut(s) 19, 1240
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 3 cut(s) 274, 341, 368
AfiI CCNNNNNNNGG 2 cut(s) 40, 1085
AgsI TTSAA 2 cut(s) 721, 814
AhlI ACTAGT 2 cut(s) 125, 269
AjnI CCWGG 4 cut(s) 955, 997, 1024, 1214
AluBI AGCT 3 cut(s) 141, 319, 775
AluI AGCT 3 cut(s) 141, 319, 775
Alw21I GWGCWC 1 cut(s) 964
Alw26I GTCTC 1 cut(s) 982
Alw44I GTGCAC 1 cut(s) 960
AlwI GGATC 1 cut(s) 776
AoxI GGCC 6 cut(s) 285, 747, 991, 1027, 1088, 1184
ApaLI GTGCAC 1 cut(s) 960
ApeKI GCWGC 2 cut(s) 257, 775
ApoI RAATTY 2 cut(s) 19, 1240
Asp700I GAANNNNTTC 1 cut(s) 615
AspLEI GCGC 2 cut(s) 455, 474
AspS9I GGNCC 5 cut(s) 992, 1001, 1012, 1088, 1195
AsuC2I CCSGG 1 cut(s) 1010
AsuHPI GGTGA 1 cut(s) 58
AvaII GGWCC 3 cut(s) 1001, 1012, 1195
BaeGI GKGCMC 2 cut(s) 964, 1167
BanI GGYRCC 3 cut(s) 278, 452, 536
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
BbsI GAAGAC 1 cut(s) 1071
Bbv12I GWGCWC 1 cut(s) 964
BbvI GCAGC 2 cut(s) 269, 762
BccI CCATC 5 cut(s) 13, 34, 189, 380, 1079
BceAI ACGGC 1 cut(s) 460
BciT130I CCWGG 4 cut(s) 957, 999, 1026, 1216
BcnI CCSGG 1 cut(s) 1010
BcoDI GTCTC 1 cut(s) 982
BcuI ACTAGT 2 cut(s) 125, 269
BfaI CTAG 5 cut(s) 126, 249, 270, 710, 1046
BfmI CTRYAG 1 cut(s) 1233
BfoI RGCGCY 1 cut(s) 456
BisI GCNGC 6 cut(s) 258, 282, 285, 288, 291, 776
BlpI GCTNAGC 1 cut(s) 137
BlsI GCNGC 6 cut(s) 259, 283, 286, 289, 292, 777
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 5 cut(s) 957, 999, 1010, 1026, 1216
Bme18I GGWCC 3 cut(s) 1001, 1012, 1195
BmgT120I GGNCC 5 cut(s) 992, 1001, 1012, 1088, 1195
BmiI GGNNCC 4 cut(s) 280, 454, 538, 942
BmrFI CCNGG 5 cut(s) 957, 999, 1010, 1026, 1216
BmsI GCATC 1 cut(s) 391
BpiI GAAGAC 1 cut(s) 1071
Bpu1102I GCTNAGC 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 1010
BsaAI YACGTR 1 cut(s) 343
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 4 cut(s) 282, 570, 955, 1214
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 1085
Bse1I ACTGG 5 cut(s) 280, 473, 702, 969, 1015
Bse3DI GCAATG 1 cut(s) 68
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 4 cut(s) 957, 999, 1026, 1216
BseDI CCNNGG 4 cut(s) 282, 570, 955, 1214
BseGI GGATG 6 cut(s) 45, 181, 391, 613, 741, 919
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 2 cut(s) 40, 1085
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 4 cut(s) 555, 576, 693, 1136
BseNI ACTGG 5 cut(s) 280, 473, 702, 969, 1015
BseRI GAGGAG 3 cut(s) 168, 171, 414
BseSI GKGCMC 2 cut(s) 964, 1167
BseX3I CGGCCG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 269, 762
BseYI CCCAGC 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 284
Bsh1285I CGRYCG 2 cut(s) 288, 984
BshFI GGCC 6 cut(s) 287, 749, 993, 1029, 1090, 1186
BshNI GGYRCC 3 cut(s) 278, 452, 536
BsiEI CGRYCG 2 cut(s) 288, 984
BsiHKAI GWGCWC 1 cut(s) 964
BsiSI CCGG 1 cut(s) 1010
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 2 cut(s) 40, 1085
BsmAI GTCTC 1 cut(s) 982
BsmFI GGGAC 1 cut(s) 708
BsnI GGCC 6 cut(s) 287, 749, 993, 1029, 1090, 1186
Bsp1286I GDGCHC 2 cut(s) 964, 1167
Bsp143I GATC 3 cut(s) 768, 930, 981
Bsp1720I GCTNAGC 1 cut(s) 137
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 5 cut(s) 222, 282, 284, 288, 291
BspANI GGCC 6 cut(s) 287, 749, 993, 1029, 1090, 1186
BspCNI CTCAG 4 cut(s) 554, 577, 692, 1135
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 4 cut(s) 280, 454, 538, 942
BspPI GGATC 1 cut(s) 776
BspQI GCTCTTC 1 cut(s) 785
BspT107I GGYRCC 3 cut(s) 278, 452, 536
BsrDI GCAATG 1 cut(s) 68
BsrI ACTGG 5 cut(s) 280, 473, 702, 969, 1015
BssECI CCNNGG 4 cut(s) 282, 570, 955, 1214
BssMI GATC 3 cut(s) 768, 930, 981
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 4 cut(s) 957, 999, 1026, 1216
Bst4CI ACNGT 5 cut(s) 52, 199, 217, 508, 631
Bst6I CTCTTC 1 cut(s) 785
BstACI GRCGYC 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 343
BstDEI CTNAG 7 cut(s) 9, 137, 541, 585, 679, 1122, 1188
BstDSI CCRYGG 2 cut(s) 282, 570
BstF5I GGATG 6 cut(s) 45, 181, 391, 613, 741, 919
BstFNI CGCG 1 cut(s) 284
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 2 cut(s) 455, 474
BstKTI GATC 3 cut(s) 771, 933, 984
BstMAI GTCTC 1 cut(s) 982
BstMBI GATC 3 cut(s) 768, 930, 981
BstMCI CGRYCG 2 cut(s) 288, 984
BstMWI GCNNNNNNNGC 5 cut(s) 91, 263, 287, 290, 968
BstNI CCWGG 4 cut(s) 957, 999, 1026, 1216
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 5 cut(s) 955, 997, 1008, 1024, 1214
BstSFI CTRYAG 1 cut(s) 1233
BstSLI GKGCMC 2 cut(s) 964, 1167
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 2 cut(s) 269, 762
BstV2I GAAGAC 1 cut(s) 1071
BstZI CGGCCG 1 cut(s) 285
BsuRI GGCC 6 cut(s) 287, 749, 993, 1029, 1090, 1186
BtgI CCRYGG 2 cut(s) 282, 570
BtsCI GGATG 6 cut(s) 45, 181, 391, 613, 741, 919
BtsI GCAGTG 1 cut(s) 1165
BtsIMutI CAGTG 3 cut(s) 466, 962, 1165
CciNI GCGGCCGC 1 cut(s) 285
CfoI GCGC 2 cut(s) 455, 474
Cfr13I GGNCC 5 cut(s) 992, 1001, 1012, 1088, 1195
Cfr42I CCGCGG 1 cut(s) 285
CseI GACGC 1 cut(s) 977
Csp6I GTAC 3 cut(s) 273, 340, 367
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 4 cut(s) 226, 571, 663, 793
CviQI GTAC 3 cut(s) 273, 340, 367
DdeI CTNAG 7 cut(s) 9, 137, 541, 585, 679, 1122, 1188
DinI GGCGCC 1 cut(s) 454
DpnI GATC 3 cut(s) 770, 932, 983
DpnII GATC 3 cut(s) 768, 930, 981
DrdI GACNNNNNNGTC 1 cut(s) 974
DseDI GACNNNNNNGTC 1 cut(s) 974
EaeI YGGCCR 1 cut(s) 285
EagI CGGCCG 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 785
EarI CTCTTC 1 cut(s) 785
EclXI CGGCCG 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 570
Eco147I AGGCCT 2 cut(s) 1029, 1186
Eco47I GGWCC 3 cut(s) 1001, 1012, 1195
Eco52I CGGCCG 1 cut(s) 285
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 4 cut(s) 955, 997, 1024, 1214
EcoT14I CCWWGG 1 cut(s) 570
EgeI GGCGCC 1 cut(s) 454
EheI GGCGCC 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 4 cut(s) 229, 574, 666, 796
FalI AAGNNNNNCTT 2 cut(s) 764, 796
FaqI GGGAC 1 cut(s) 708
FatI CATG 4 cut(s) 225, 570, 662, 792
FauI CCCGC 1 cut(s) 229
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 6 cut(s) 258, 282, 285, 288, 291, 776
FokI GGATG 6 cut(s) 52, 168, 378, 620, 748, 926
Fsp4HI GCNGC 6 cut(s) 258, 282, 285, 288, 291, 776
FspBI CTAG 5 cut(s) 126, 249, 270, 710, 1046
GlaI GCGC 2 cut(s) 454, 473
GluI GCNGC 6 cut(s) 258, 282, 285, 288, 291, 776
GsaI CCCAGC 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 6 cut(s) 287, 749, 993, 1029, 1090, 1186
HapII CCGG 1 cut(s) 1010
HgaI GACGC 1 cut(s) 977
HhaI GCGC 2 cut(s) 455, 474
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 4 cut(s) 229, 574, 666, 796
Hin6I GCGC 2 cut(s) 453, 472
HinP1I GCGC 2 cut(s) 453, 472
HinfI GANTC 5 cut(s) 159, 412, 683, 905, 1042
HpaII CCGG 1 cut(s) 1010
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 6 cut(s) 149, 442, 892, 962, 1074, 1198
Hpy188I TCNGA 6 cut(s) 25, 411, 544, 621, 682, 1125
Hpy188III TCNNGA 3 cut(s) 185, 395, 979
Hpy8I GTNNAC 6 cut(s) 149, 442, 892, 962, 1074, 1198
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 3 cut(s) 352, 844, 1128
HpyCH4III ACNGT 5 cut(s) 52, 199, 217, 508, 631
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 5 cut(s) 85, 257, 404, 662, 962
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 263, 287, 290, 968
HpyF3I CTNAG 7 cut(s) 9, 137, 541, 585, 679, 1122, 1188
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 4 cut(s) 229, 574, 666, 796
HspAI GCGC 2 cut(s) 453, 472
KasI GGCGCC 1 cut(s) 452
KspI CCGCGG 1 cut(s) 285
Kzo9I GATC 3 cut(s) 768, 930, 981
LguI GCTCTTC 1 cut(s) 785
LmnI GCTCC 2 cut(s) 316, 427
Lsp1109I GCAGC 2 cut(s) 269, 762
LweI GCATC 1 cut(s) 391
MaeI CTAG 5 cut(s) 126, 249, 270, 710, 1046
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 3 cut(s) 46, 950, 985
MalI GATC 3 cut(s) 770, 932, 983
MboI GATC 3 cut(s) 768, 930, 981
MboII GAAGA 6 cut(s) 410, 425, 772, 929, 1076, 1202
MhlI GDGCHC 2 cut(s) 964, 1167
MluCI AATT 6 cut(s) 19, 311, 721, 798, 920, 1240
Mly113I GGCGCC 1 cut(s) 453
MlyI GAGTC 1 cut(s) 168
MmeI TCCRAC 2 cut(s) 288, 1200
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 8 cut(s) 201, 234, 330, 483, 590, 635, 869, 1019
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 1 cut(s) 1010
MspR9I CCNGG 5 cut(s) 957, 999, 1010, 1026, 1216
MvaI CCWGG 4 cut(s) 957, 999, 1026, 1216
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 5 cut(s) 91, 263, 287, 290, 968
NarI GGCGCC 1 cut(s) 453
NciI CCSGG 1 cut(s) 1010
NcoI CCATGG 1 cut(s) 570
NdeII GATC 3 cut(s) 768, 930, 981
NlaIII CATG 4 cut(s) 229, 574, 666, 796
NlaIV GGNNCC 4 cut(s) 280, 454, 538, 942
NmuCI GTSAC 2 cut(s) 46, 950
NotI GCGGCCGC 1 cut(s) 285
NspI RCATGY 1 cut(s) 666
PceI AGGCCT 2 cut(s) 1029, 1186
PciSI GCTCTTC 1 cut(s) 785
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 4 cut(s) 412, 683, 905, 1042
PflMI CCANNNNNTGG 2 cut(s) 40, 1085
PkrI GCNGC 6 cut(s) 259, 283, 286, 289, 292, 777
Ple19I CGATCG 1 cut(s) 984
PleI GAGTC 1 cut(s) 167
PluTI GGCGCC 1 cut(s) 456
PpsI GAGTC 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 343
Psp6I CCWGG 4 cut(s) 955, 997, 1024, 1214
PspFI CCCAGC 1 cut(s) 319
PspGI CCWGG 4 cut(s) 955, 997, 1024, 1214
PspN4I GGNNCC 4 cut(s) 280, 454, 538, 942
PspPI GGNCC 5 cut(s) 992, 1001, 1012, 1088, 1195
PvuI CGATCG 1 cut(s) 984
RsaI GTAC 3 cut(s) 274, 341, 368
RsaNI GTAC 3 cut(s) 273, 340, 367
SacII CCGCGG 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 785
SaqAI TTAA 8 cut(s) 201, 234, 330, 483, 590, 635, 869, 1019
SatI GCNGC 6 cut(s) 258, 282, 285, 288, 291, 776
Sau3AI GATC 3 cut(s) 768, 930, 981
Sau96I GGNCC 5 cut(s) 992, 1001, 1012, 1088, 1195
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 5 cut(s) 957, 999, 1010, 1026, 1216
SduI GDGCHC 2 cut(s) 964, 1167
SetI ASST 8 cut(s) 143, 321, 345, 525, 777, 826, 961, 1120
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 1233
SfoI GGCGCC 1 cut(s) 454
Sfr303I CCGCGG 1 cut(s) 285
SgrBI CCGCGG 1 cut(s) 285
SinI GGWCC 3 cut(s) 1001, 1012, 1195
SpeI ACTAGT 2 cut(s) 125, 269
Sse9I AATT 6 cut(s) 19, 311, 721, 798, 920, 1240
SseBI AGGCCT 2 cut(s) 1029, 1186
SsiI CCGC 5 cut(s) 222, 282, 284, 288, 291
SspDI GGCGCC 1 cut(s) 452
SspI AATATT 2 cut(s) 80, 481
SspMI CTAG 5 cut(s) 126, 249, 270, 710, 1046
StuI AGGCCT 2 cut(s) 1029, 1186
StyD4I CCNGG 5 cut(s) 955, 997, 1008, 1024, 1214
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 5 cut(s) 52, 199, 217, 508, 631
TaiI ACGT 1 cut(s) 345
TaqI TCGA 5 cut(s) 98, 703, 980, 1083, 1130
TasI AATT 6 cut(s) 19, 311, 721, 798, 920, 1240
TatI WGTACW 1 cut(s) 272
TauI GCSGC 4 cut(s) 284, 287, 290, 293
TfiI GAWTC 4 cut(s) 412, 683, 905, 1042
Tru1I TTAA 8 cut(s) 201, 234, 330, 483, 590, 635, 869, 1019
Tru9I TTAA 8 cut(s) 201, 234, 330, 483, 590, 635, 869, 1019
TscAI CASTG 3 cut(s) 473, 969, 1172
TseFI GTSAC 2 cut(s) 46, 950
TseI GCWGC 2 cut(s) 257, 775
Tsp45I GTSAC 2 cut(s) 46, 950
TspDTI ATGAA 8 cut(s) 57, 242, 552, 624, 752, 826, 930, 1077
TspGWI ACGGA 1 cut(s) 448
TspRI CASTG 3 cut(s) 473, 969, 1172
Van91I CCANNNNNTGG 2 cut(s) 40, 1085
VneI GTGCAC 1 cut(s) 960
VpaK11BI GGWCC 3 cut(s) 1001, 1012, 1195
XapI RAATTY 2 cut(s) 19, 1240
XceI RCATGY 1 cut(s) 666
XmiI GTMKAC 1 cut(s) 148
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 5 cut(s) 126, 249, 270, 710, 1046
ZrmI AGTACT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.