Rh6DG444700

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0015325
Physical Location & Seq
Reverse (-)
61917801 .. 61921142
3342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG444700.1

Sequence Viewer

Length: 1326 bp
ATGATCTCGTCAAATAGAATTGTAAAGCTAGGCAAAGACACAGCTGGCCTGGCATATATAAAAGAAAGAAGTAAATACATGCCATCTAAGAGTGAGAATTCAGATAGAAACCATAGGATGGGGGTGACGGTAATATCTCCCCATTCATTGCCACAATATTTGCATTTACAGCAGTCGATACCAAACCAGAAAGCAGTAAAAACTAGTTGTTTGCTTAGCTATGGTGGACGAGGAGGAATCTTGCTTGGTCAAAATAACCATCCAGACCCGAGTCACCCGACCACCGTTAATAGTATCACTACTCTTCCCGCCATGAAACTTAACTCATCATCATCTAGTATTGCAGCAGAGGCAACTAGTACTGGTGCCGCGGCCGCCGCCGACGATGTAGTGTTGGAATTGGAGCTGGGAGAGATTAAGGACAAGTACATGAAGTGGAAGTGGAAGGGTCAGTACGCCATCAACTACATCGTTTCACATCCAGAAGATGCAAATCCGAATCCAAATGCTCTTCCTATTCTTCTTGTTCACGGCTTCGGCGCCTCCGTTCCTCACTGGCGCAGAAATATTAAGACATTGGCGAAGAATTATACAGTTTATGCTATTGACCTGATTGGCTTTGGTGCCTCAGATAAGCCGATAGGCTTTTCATACACCATGGAGAAATGGGCTGAGTTAATCTTGGATTTTTTGGATGAAGTTGTTCAGAAGCCTACTGTTTTGATAGGCAACTCTGTGGGAAGCCTTGCATGTGTAATCTCTGCCTCAGAATCTAAAAAGGGACTGGTTCGAGGGCTAGTGCTTTTAAATTGCTCTGGTGGGATGAATAACAAGGCCATTGTTGATGATTGGAGGATCAAGCTGCTCTTCCCTTTGCTCTTGCTAATTGATTTCTTGTTGAAGCAACGACCTATTGCTTCCTTCATTTTTGAGCGTGTTAGACAGAGGGAAAGTTTAAGAAATATCTTATCATCTGTTTACGCCAACAAAGAATCTGTGGATGACGAATTAGTAGAGATCATAATGGAACCAACAAGCGACCCAGGTGCACTGGACGCGTTTGTCTCAATCGTCACAGGCCCACCAGGACCAAATCCGGTCCAGTTGATGCCGGGCCTCTCCCTACCGATTCTAGTCTTATGGGGCGATGAAGACCCGTTCACTCCGCTTGATGGGCCTGTTGGCAAATACTTCACTTCTTTACCTTCACAGAAATCGAATGTGAGCCTCTCCATTTTACAAGGAGTTGGGCACTGCCCCCACGATGACAGGCCCAAGTTGGTCCACGACAAACTTCTTCCCTGGTTGGATAATCTTTCTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

441

Amino Acids

48.25

Weight (kDa)

7.17

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 172 - 425 9.1e-24 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 173 - 429 9.2e-21 Alpha/beta hydrolase family
Hydrolase_4 PF12146 173 - 423 1.8e-10 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38520 AT5G38520
fragaria_vesca FvH4_2g37190
malus_domestica MD08G1001000.v1.1
prunus_persica Prupe.1G353100_v2.0.a1
pyrus_communis pycom08g00040
rosa_chinensis RchiOBHm_Chr6g0304501
rosa_laevigata RLG00000010971
rosa_multiflora Rmu_sc0023263.1_g000001 Rmu_ssc0000218.1_g000058
rosa_roxburghii Rroxscaffold_7G00163690
rosa_rugosa Rorug06G0332600
rosa_samantha Rh6AG445100 Rh6CG457900 Rh6DG444700
rosa_wichuraiana Rw6G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1061
AccB1I GGYRCC 3 cut(s) 365, 539, 623
AccB7I CCANNNNNTGG 1 cut(s) 118
AccII CGCG 2 cut(s) 371, 1058
AciI CCGC 6 cut(s) 309, 369, 371, 375, 378, 1166
AclWI GGATC 1 cut(s) 863
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 1 cut(s) 97
AcyI GRCGYC 1 cut(s) 540
AfaI GTAC 3 cut(s) 361, 428, 455
AfiI CCNNNNNNNGG 2 cut(s) 118, 1172
AflIII ACRYGT 1 cut(s) 1056
AgsI TTSAA 1 cut(s) 901
AhlI ACTAGT 2 cut(s) 203, 356
AjnI CCWGG 4 cut(s) 48, 1042, 1084, 1301
AluBI AGCT 5 cut(s) 28, 44, 219, 406, 862
AluI AGCT 5 cut(s) 28, 44, 219, 406, 862
Alw21I GWGCWC 1 cut(s) 1051
Alw26I GTCTC 1 cut(s) 1069
Alw44I GTGCAC 1 cut(s) 1047
AlwI GGATC 1 cut(s) 863
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 7 cut(s) 46, 372, 834, 1078, 1114, 1175, 1271
ApaLI GTGCAC 1 cut(s) 1047
ApeKI GCWGC 2 cut(s) 344, 862
ApoI RAATTY 1 cut(s) 97
Asp700I GAANNNNTTC 1 cut(s) 702
AspLEI GCGC 2 cut(s) 542, 561
AspS9I GGNCC 7 cut(s) 1079, 1088, 1099, 1114, 1175, 1272, 1282
AsuC2I CCSGG 1 cut(s) 1113
AsuHPI GGTGA 2 cut(s) 136, 266
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 3 cut(s) 1088, 1099, 1282
BaeGI GKGCMC 2 cut(s) 1051, 1254
BanI GGYRCC 3 cut(s) 365, 539, 623
BarI GAAGNNNNNNTAC 2 cut(s) 437, 469
BbsI GAAGAC 1 cut(s) 1158
Bbv12I GWGCWC 1 cut(s) 1051
BbvI GCAGC 2 cut(s) 356, 849
BccI CCATC 5 cut(s) 91, 112, 267, 467, 1166
BceAI ACGGC 1 cut(s) 547
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BciT130I CCWGG 4 cut(s) 50, 1044, 1086, 1303
BcnI CCSGG 1 cut(s) 1113
BcoDI GTCTC 1 cut(s) 1069
BcuI ACTAGT 2 cut(s) 203, 356
BfaI CTAG 6 cut(s) 29, 204, 336, 357, 797, 1133
BfoI RGCGCY 1 cut(s) 543
BisI GCNGC 6 cut(s) 345, 369, 372, 375, 378, 863
BlpI GCTNAGC 1 cut(s) 215
BlsI GCNGC 6 cut(s) 346, 370, 373, 376, 379, 864
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 5 cut(s) 50, 1044, 1086, 1113, 1303
Bme18I GGWCC 3 cut(s) 1088, 1099, 1282
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 7 cut(s) 1079, 1088, 1099, 1114, 1175, 1272, 1282
BmiI GGNNCC 4 cut(s) 367, 541, 625, 1029
BmrFI CCNGG 5 cut(s) 50, 1044, 1086, 1113, 1303
BmsI GCATC 2 cut(s) 478, 1098
BoxI GACNNNNGTC 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 1158
Bpu1102I GCTNAGC 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 1113
BsaBI GATNNNNATC 1 cut(s) 492
BsaHI GRCGYC 1 cut(s) 540
BsaJI CCNNGG 4 cut(s) 369, 657, 1042, 1301
BsaWI WCCGGW 1 cut(s) 1096
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 1172
Bse1I ACTGG 5 cut(s) 367, 560, 789, 1056, 1102
Bse3DI GCAATG 1 cut(s) 146
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 4 cut(s) 50, 1044, 1086, 1303
BseDI CCNNGG 4 cut(s) 369, 657, 1042, 1301
BseGI GGATG 6 cut(s) 123, 259, 478, 700, 828, 1006
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 2 cut(s) 118, 1172
BseMI GCAATG 1 cut(s) 146
BseMII CTCAG 3 cut(s) 642, 663, 780
BseNI ACTGG 5 cut(s) 367, 560, 789, 1056, 1102
BseRI GAGGAG 1 cut(s) 246
BseSI GKGCMC 2 cut(s) 1051, 1254
BseX3I CGGCCG 1 cut(s) 372
BseXI GCAGC 2 cut(s) 356, 849
BseYI CCCAGC 1 cut(s) 406
Bsh1236I CGCG 2 cut(s) 371, 1058
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 7 cut(s) 48, 374, 836, 1080, 1116, 1177, 1273
BshNI GGYRCC 3 cut(s) 365, 539, 623
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 1051
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 2 cut(s) 1097, 1112
BslFI GGGAC 1 cut(s) 795
BslI CCNNNNNNNGG 2 cut(s) 118, 1172
BsmAI GTCTC 1 cut(s) 1069
BsmFI GGGAC 1 cut(s) 795
BsnI GGCC 7 cut(s) 48, 374, 836, 1080, 1116, 1177, 1273
BsoBI CYCGRG 1 cut(s) 268
Bsp1286I GDGCHC 2 cut(s) 1051, 1254
Bsp143I GATC 3 cut(s) 3, 855, 1017
Bsp1720I GCTNAGC 1 cut(s) 215
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 6 cut(s) 309, 369, 371, 375, 378, 1166
BspANI GGCC 7 cut(s) 48, 374, 836, 1080, 1116, 1177, 1273
BspCNI CTCAG 3 cut(s) 641, 664, 779
BspFNI CGCG 2 cut(s) 371, 1058
BspLI GGNNCC 4 cut(s) 367, 541, 625, 1029
BspPI GGATC 1 cut(s) 863
BspQI GCTCTTC 2 cut(s) 516, 872
BspT107I GGYRCC 3 cut(s) 365, 539, 623
BsrDI GCAATG 1 cut(s) 146
BsrI ACTGG 5 cut(s) 367, 560, 789, 1056, 1102
BssECI CCNNGG 4 cut(s) 369, 657, 1042, 1301
BssMI GATC 3 cut(s) 3, 855, 1017
BssNI GRCGYC 1 cut(s) 540
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 4 cut(s) 50, 1044, 1086, 1303
Bst4CI ACNGT 4 cut(s) 130, 286, 595, 718
Bst6I CTCTTC 3 cut(s) 309, 516, 872
BstACI GRCGYC 1 cut(s) 540
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 5 cut(s) 87, 215, 628, 672, 766
BstDSI CCRYGG 2 cut(s) 369, 657
BstF5I GGATG 6 cut(s) 123, 259, 478, 700, 828, 1006
BstFNI CGCG 2 cut(s) 371, 1058
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 2 cut(s) 542, 561
BstKTI GATC 3 cut(s) 6, 858, 1020
BstMAI GTCTC 1 cut(s) 1069
BstMBI GATC 3 cut(s) 3, 855, 1017
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 7 cut(s) 50, 169, 350, 374, 377, 1055, 1174
BstNI CCWGG 4 cut(s) 50, 1044, 1086, 1303
BstNSI RCATGY 2 cut(s) 82, 753
BstPAI GACNNNNGTC 1 cut(s) 270
BstSCI CCNGG 5 cut(s) 48, 1042, 1084, 1111, 1301
BstSLI GKGCMC 2 cut(s) 1051, 1254
BstUI CGCG 2 cut(s) 371, 1058
BstV1I GCAGC 2 cut(s) 356, 849
BstV2I GAAGAC 1 cut(s) 1158
BstZI CGGCCG 1 cut(s) 372
BsuRI GGCC 7 cut(s) 48, 374, 836, 1080, 1116, 1177, 1273
BtgI CCRYGG 2 cut(s) 369, 657
BtgZI GCGATG 1 cut(s) 1161
BtsCI GGATG 6 cut(s) 123, 259, 478, 700, 828, 1006
BtsI GCAGTG 1 cut(s) 1252
BtsIMutI CAGTG 3 cut(s) 553, 1049, 1252
Cac8I GCNNGC 1 cut(s) 46
CciNI GCGGCCGC 1 cut(s) 372
CfoI GCGC 2 cut(s) 542, 561
Cfr13I GGNCC 7 cut(s) 1079, 1088, 1099, 1114, 1175, 1272, 1282
Cfr42I CCGCGG 1 cut(s) 372
CseI GACGC 1 cut(s) 1064
Csp6I GTAC 3 cut(s) 360, 427, 454
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviAII CATG 5 cut(s) 79, 313, 430, 658, 750
CviQI GTAC 3 cut(s) 360, 427, 454
DdeI CTNAG 5 cut(s) 87, 215, 628, 672, 766
DinI GGCGCC 1 cut(s) 541
DpnI GATC 3 cut(s) 5, 857, 1019
DpnII GATC 3 cut(s) 3, 855, 1017
DraI TTTAAA 1 cut(s) 807
DrdI GACNNNNNNGTC 1 cut(s) 1061
DseDI GACNNNNNNGTC 1 cut(s) 1061
EaeI YGGCCR 1 cut(s) 372
EagI CGGCCG 1 cut(s) 372
Eam1104I CTCTTC 3 cut(s) 309, 516, 872
EarI CTCTTC 3 cut(s) 309, 516, 872
EclXI CGGCCG 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 657
Eco47I GGWCC 3 cut(s) 1088, 1099, 1282
Eco52I CGGCCG 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 97
EcoRII CCWGG 4 cut(s) 48, 1042, 1084, 1301
EcoT14I CCWWGG 1 cut(s) 657
EgeI GGCGCC 1 cut(s) 541
EheI GGCGCC 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 5 cut(s) 82, 316, 433, 661, 753
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FaqI GGGAC 1 cut(s) 795
FatI CATG 5 cut(s) 78, 312, 429, 657, 749
FauI CCCGC 1 cut(s) 316
Fnu4HI GCNGC 6 cut(s) 345, 369, 372, 375, 378, 863
FokI GGATG 6 cut(s) 130, 246, 465, 707, 835, 1013
Fsp4HI GCNGC 6 cut(s) 345, 369, 372, 375, 378, 863
FspBI CTAG 6 cut(s) 29, 204, 336, 357, 797, 1133
GlaI GCGC 2 cut(s) 541, 560
GluI GCNGC 6 cut(s) 345, 369, 372, 375, 378, 863
GsaI CCCAGC 1 cut(s) 410
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 7 cut(s) 48, 374, 836, 1080, 1116, 1177, 1273
HapII CCGG 2 cut(s) 1097, 1112
HgaI GACGC 1 cut(s) 1064
HhaI GCGC 2 cut(s) 542, 561
Hin1I GRCGYC 1 cut(s) 540
Hin1II CATG 5 cut(s) 82, 316, 433, 661, 753
Hin6I GCGC 2 cut(s) 540, 559
HinP1I GCGC 2 cut(s) 540, 559
HinfI GANTC 6 cut(s) 237, 271, 499, 770, 992, 1129
HpaII CCGG 2 cut(s) 1097, 1112
HphI GGTGA 2 cut(s) 136, 266
Hpy166II GTNNAC 6 cut(s) 227, 529, 979, 1049, 1161, 1285
Hpy188I TCNGA 5 cut(s) 103, 498, 631, 708, 769
Hpy188III TCNNGA 2 cut(s) 263, 482
Hpy8I GTNNAC 6 cut(s) 227, 529, 979, 1049, 1161, 1285
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 3 cut(s) 439, 931, 1215
HpyCH4III ACNGT 4 cut(s) 130, 286, 595, 718
HpyCH4V TGCA 5 cut(s) 163, 344, 491, 749, 1049
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 169, 350, 374, 377, 1055, 1174
HpyF3I CTNAG 5 cut(s) 87, 215, 628, 672, 766
Hsp92I GRCGYC 1 cut(s) 540
Hsp92II CATG 5 cut(s) 82, 316, 433, 661, 753
HspAI GCGC 2 cut(s) 540, 559
KasI GGCGCC 1 cut(s) 539
KspI CCGCGG 1 cut(s) 372
Kzo9I GATC 3 cut(s) 3, 855, 1017
LguI GCTCTTC 2 cut(s) 516, 872
LmnI GCTCC 1 cut(s) 403
Lsp1109I GCAGC 2 cut(s) 356, 849
LweI GCATC 2 cut(s) 478, 1098
MaeI CTAG 6 cut(s) 29, 204, 336, 357, 797, 1133
MaeIII GTNAC 3 cut(s) 124, 272, 1072
MalI GATC 3 cut(s) 5, 857, 1019
MboI GATC 3 cut(s) 3, 855, 1017
MboII GAAGA 8 cut(s) 296, 497, 503, 512, 595, 859, 1163, 1289
MhlI GDGCHC 2 cut(s) 1051, 1254
MluCI AATT 7 cut(s) 18, 97, 398, 586, 808, 885, 1007
MluI ACGCGT 1 cut(s) 1056
Mly113I GGCGCC 1 cut(s) 540
MlyI GAGTC 1 cut(s) 280
MmeI TCCRAC 2 cut(s) 375, 1287
MroXI GAANNNNTTC 1 cut(s) 702
MseI TTAA 8 cut(s) 288, 321, 417, 570, 677, 806, 956, 1324
MspA1I CMGCKG 2 cut(s) 44, 371
MspI CCGG 2 cut(s) 1097, 1112
MspR9I CCNGG 5 cut(s) 50, 1044, 1086, 1113, 1303
MvaI CCWGG 4 cut(s) 50, 1044, 1086, 1303
MvnI CGCG 2 cut(s) 371, 1058
MwoI GCNNNNNNNGC 7 cut(s) 50, 169, 350, 374, 377, 1055, 1174
NarI GGCGCC 1 cut(s) 540
NciI CCSGG 1 cut(s) 1113
NcoI CCATGG 1 cut(s) 657
NdeII GATC 3 cut(s) 3, 855, 1017
NlaIII CATG 5 cut(s) 82, 316, 433, 661, 753
NlaIV GGNNCC 4 cut(s) 367, 541, 625, 1029
NmuCI GTSAC 3 cut(s) 124, 272, 1072
NotI GCGGCCGC 1 cut(s) 372
NspI RCATGY 2 cut(s) 82, 753
PciSI GCTCTTC 2 cut(s) 516, 872
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 702
PfeI GAWTC 5 cut(s) 237, 499, 770, 992, 1129
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 6 cut(s) 346, 370, 373, 376, 379, 864
PleI GAGTC 1 cut(s) 279
PluTI GGCGCC 1 cut(s) 543
PpsI GAGTC 1 cut(s) 279
PshAI GACNNNNGTC 1 cut(s) 270
Psp6I CCWGG 4 cut(s) 48, 1042, 1084, 1301
PspFI CCCAGC 1 cut(s) 406
PspGI CCWGG 4 cut(s) 48, 1042, 1084, 1301
PspN4I GGNNCC 4 cut(s) 367, 541, 625, 1029
PspPI GGNCC 7 cut(s) 1079, 1088, 1099, 1114, 1175, 1272, 1282
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 3 cut(s) 361, 428, 455
RsaNI GTAC 3 cut(s) 360, 427, 454
SacII CCGCGG 1 cut(s) 372
SapI GCTCTTC 2 cut(s) 516, 872
SaqAI TTAA 8 cut(s) 288, 321, 417, 570, 677, 806, 956, 1324
SatI GCNGC 6 cut(s) 345, 369, 372, 375, 378, 863
Sau3AI GATC 3 cut(s) 3, 855, 1017
Sau96I GGNCC 7 cut(s) 1079, 1088, 1099, 1114, 1175, 1272, 1282
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 5 cut(s) 50, 1044, 1086, 1113, 1303
SduI GDGCHC 2 cut(s) 1051, 1254
SetI ASST 9 cut(s) 30, 46, 221, 408, 612, 864, 913, 1048, 1207
SfaNI GCATC 2 cut(s) 478, 1098
SfoI GGCGCC 1 cut(s) 541
Sfr303I CCGCGG 1 cut(s) 372
SgrBI CCGCGG 1 cut(s) 372
SinI GGWCC 3 cut(s) 1088, 1099, 1282
SpeI ACTAGT 2 cut(s) 203, 356
Sse9I AATT 7 cut(s) 18, 97, 398, 586, 808, 885, 1007
SsiI CCGC 6 cut(s) 309, 369, 371, 375, 378, 1166
SspDI GGCGCC 1 cut(s) 539
SspI AATATT 2 cut(s) 158, 568
SspMI CTAG 6 cut(s) 29, 204, 336, 357, 797, 1133
StyD4I CCNGG 5 cut(s) 48, 1042, 1084, 1111, 1301
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 4 cut(s) 130, 286, 595, 718
TaqI TCGA 3 cut(s) 176, 790, 1217
TasI AATT 7 cut(s) 18, 97, 398, 586, 808, 885, 1007
TatI WGTACW 2 cut(s) 359, 426
TauI GCSGC 4 cut(s) 371, 374, 377, 380
TfiI GAWTC 5 cut(s) 237, 499, 770, 992, 1129
Tru1I TTAA 8 cut(s) 288, 321, 417, 570, 677, 806, 956, 1324
Tru9I TTAA 8 cut(s) 288, 321, 417, 570, 677, 806, 956, 1324
TscAI CASTG 3 cut(s) 560, 1056, 1259
TseFI GTSAC 3 cut(s) 124, 272, 1072
TseI GCWGC 2 cut(s) 344, 862
Tsp45I GTSAC 3 cut(s) 124, 272, 1072
TspDTI ATGAA 8 cut(s) 135, 329, 446, 639, 711, 839, 913, 1164
TspGWI ACGGA 1 cut(s) 535
TspRI CASTG 3 cut(s) 560, 1056, 1259
Van91I CCANNNNNTGG 1 cut(s) 118
VneI GTGCAC 1 cut(s) 1047
VpaK11BI GGWCC 3 cut(s) 1088, 1099, 1282
XapI RAATTY 1 cut(s) 97
XceI RCATGY 2 cut(s) 82, 753
XmnI GAANNNNTTC 1 cut(s) 702
XspI CTAG 6 cut(s) 29, 204, 336, 357, 797, 1133
ZrmI AGTACT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.