Rh6CG457900

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0015325
Physical Location & Seq
Reverse (-)
63277242 .. 63279820
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG457900.1

Sequence Viewer

Length: 1263 bp
ATGCCATCTAAGAGTGAGAATTCAGATAGAAACCATAGGATGGGGGTGACGGTAATATCTCCCCATTCATTGCCACAATATTTGCATTTACAGCAGTCGATACCAAACCAGAAAGCAGTAAAAACTAGTTGTTTGCTTTGCTATGGTGGACGAGGAGGAGTCTTGCTTGGTCAAAATAACCATCCAGACCCGAGTCACCCGACCACCGTTAATAGTATCACTACTCTTCCCGCCATGAAACTTAACTCATCATCATCTAGTATTGCAGCAGAGGCAACTAGTACTGGTGCCGCGGCCGCCGCCGACGATGTAGTGTTGGAATTGGAGCTGGGAGAGATTAAGGACAAGTACATGAAGTGGAAGTGGAAGGGTCAGTACGCCATCAACTACATCGTTTCACATCCAGAAGATGCAAATCCGAATCCAAATGCTCCTCCTATTCTTCTTGTTCACGGCTTCGGCGCCTCCGTTCCTCACTGGCGCAGAAATATTAAGACATTGGCGAAGCATTATACAGTTTATGCAATTGACCTGATTGGCTTTGGTGCCTCAGATAAGCCGATAGGCTTTTCATATACCATGGAGAAATGGGCTGAGTTAATCTTGGATTTTTTGGATGAAGTTGTTCAGAAGCCTACTGTTTTGATAGGCAACTCTGTGGGAAGCCTTGCATGTGTAATCTCTGCCTCAGAATCTAAAAAGGGACTGGTTCGAGGGCTAGTGCTTTTAAATTGCTCTGGTGGGATGAATAACAAGGCCATTGTTGATGATTGGAGGATCAAGCTGCTCTTCCCTTTGCTCTTGCTAATTGATTTCTTGTTGAAGCAACGACCTATTGCTTCCTTCATTTTTGAGCGTGTTAGACAGAGGGAAAGTTTAAGAAATATCTTATCATCTGTTTACGCTAACAAAGAATCTGTGGATGACGAATTAGTAGAGATCATAATGGAACCAACAAGTGACCCAGGTGCACTGGACGCGTTTGTCTCAATCGTAACAGGGCCACCAGGACCAAACCCGGTCCAGTTAATGCCAGGCCTCTCCCTACCGATTCTAGTCCTATGGGGCGATGAAGACCCGTTCACTCCACTCGATGGGCCTGTTGGCAAATACTTCACTTCCTTACCTTCTCAGAAATCGAATGTGAGCCTCTCCATTTTACAAGGAGTTGGGCACTGCCCCCACGATGACAGGCCCAAGTTGGTCCACGACAAACTTCTTGCCTGGTTGGATAATCTTTCTATAGAAAATCCAGTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

420

Amino Acids

45.84

Weight (kDa)

6.18

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 145 - 399 1.9e-25 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 147 - 403 2.5e-21 Alpha/beta hydrolase family
Hydrolase_4 PF12146 147 - 397 3e-10 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38520 AT5G38520
fragaria_vesca FvH4_2g37190
malus_domestica MD08G1001000.v1.1
prunus_persica Prupe.1G353100_v2.0.a1
pyrus_communis pycom08g00040
rosa_chinensis RchiOBHm_Chr6g0304501
rosa_laevigata RLG00000010971
rosa_multiflora Rmu_sc0023263.1_g000001 Rmu_ssc0000218.1_g000058
rosa_roxburghii Rroxscaffold_7G00163690
rosa_rugosa Rorug06G0332600
rosa_samantha Rh6AG445100 Rh6CG457900 Rh6DG444700
rosa_wichuraiana Rw6G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 983
AccB1I GGYRCC 3 cut(s) 287, 461, 545
AccB7I CCANNNNNTGG 2 cut(s) 40, 1094
AccII CGCG 2 cut(s) 293, 980
AciI CCGC 5 cut(s) 231, 291, 293, 297, 300
AclWI GGATC 1 cut(s) 785
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 1 cut(s) 19
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 3 cut(s) 283, 350, 377
AfiI CCNNNNNNNGG 2 cut(s) 40, 1094
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 1 cut(s) 823
AhlI ACTAGT 2 cut(s) 125, 278
AjnI CCWGG 4 cut(s) 964, 1006, 1033, 1223
AluBI AGCT 2 cut(s) 328, 784
AluI AGCT 2 cut(s) 328, 784
Alw21I GWGCWC 1 cut(s) 973
Alw26I GTCTC 1 cut(s) 991
Alw44I GTGCAC 1 cut(s) 969
AlwI GGATC 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 190
AoxI GGCC 6 cut(s) 294, 756, 1001, 1036, 1097, 1193
ApaLI GTGCAC 1 cut(s) 969
ApeKI GCWGC 2 cut(s) 266, 784
ApoI RAATTY 1 cut(s) 19
Asp700I GAANNNNTTC 1 cut(s) 624
AspLEI GCGC 2 cut(s) 464, 483
AspS9I GGNCC 6 cut(s) 1001, 1010, 1021, 1097, 1194, 1204
AsuC2I CCSGG 1 cut(s) 1019
AsuHPI GGTGA 2 cut(s) 58, 188
AvaI CYCGRG 1 cut(s) 190
AvaII GGWCC 3 cut(s) 1010, 1021, 1204
BaeGI GKGCMC 2 cut(s) 973, 1176
BanI GGYRCC 3 cut(s) 287, 461, 545
BarI GAAGNNNNNNTAC 2 cut(s) 359, 391
BbsI GAAGAC 1 cut(s) 1080
Bbv12I GWGCWC 1 cut(s) 973
BbvI GCAGC 2 cut(s) 278, 771
BccI CCATC 5 cut(s) 13, 34, 189, 389, 1088
BceAI ACGGC 1 cut(s) 469
BciT130I CCWGG 4 cut(s) 966, 1008, 1035, 1225
BcnI CCSGG 1 cut(s) 1019
BcoDI GTCTC 1 cut(s) 991
BcuI ACTAGT 2 cut(s) 125, 278
BfaI CTAG 5 cut(s) 126, 258, 279, 719, 1055
BfmI CTRYAG 1 cut(s) 1242
BfoI RGCGCY 1 cut(s) 465
BisI GCNGC 6 cut(s) 267, 291, 294, 297, 300, 785
BlsI GCNGC 6 cut(s) 268, 292, 295, 298, 301, 786
BmcAI AGTACT 1 cut(s) 283
Bme1390I CCNGG 5 cut(s) 966, 1008, 1019, 1035, 1225
Bme18I GGWCC 3 cut(s) 1010, 1021, 1204
BmeT110I CYCGRG 1 cut(s) 190
BmgT120I GGNCC 6 cut(s) 1001, 1010, 1021, 1097, 1194, 1204
BmiI GGNNCC 4 cut(s) 289, 463, 547, 951
BmrFI CCNGG 5 cut(s) 966, 1008, 1019, 1035, 1225
BmsI GCATC 1 cut(s) 400
BoxI GACNNNNGTC 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 1080
BpuMI CCSGG 1 cut(s) 1019
BsaBI GATNNNNATC 1 cut(s) 414
BsaHI GRCGYC 1 cut(s) 462
BsaJI CCNNGG 3 cut(s) 291, 579, 964
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 1094
Bse1I ACTGG 6 cut(s) 289, 482, 711, 978, 1024, 1253
Bse3DI GCAATG 1 cut(s) 68
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 4 cut(s) 966, 1008, 1035, 1225
BseDI CCNNGG 3 cut(s) 291, 579, 964
BseGI GGATG 6 cut(s) 45, 181, 400, 622, 750, 928
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 40, 1094
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 4 cut(s) 564, 585, 702, 1145
BseNI ACTGG 6 cut(s) 289, 482, 711, 978, 1024, 1253
BseRI GAGGAG 3 cut(s) 168, 171, 423
BseSI GKGCMC 2 cut(s) 973, 1176
BseX3I CGGCCG 1 cut(s) 294
BseXI GCAGC 2 cut(s) 278, 771
BseYI CCCAGC 1 cut(s) 328
Bsh1236I CGCG 2 cut(s) 293, 980
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 6 cut(s) 296, 758, 1003, 1038, 1099, 1195
BshNI GGYRCC 3 cut(s) 287, 461, 545
BsiEI CGRYCG 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 973
BsiHKCI CYCGRG 1 cut(s) 190
BsiSI CCGG 1 cut(s) 1019
BslFI GGGAC 1 cut(s) 717
BslI CCNNNNNNNGG 2 cut(s) 40, 1094
BsmAI GTCTC 1 cut(s) 991
BsmFI GGGAC 1 cut(s) 717
BsnI GGCC 6 cut(s) 296, 758, 1003, 1038, 1099, 1195
BsoBI CYCGRG 1 cut(s) 190
Bsp1286I GDGCHC 2 cut(s) 973, 1176
Bsp143I GATC 2 cut(s) 777, 939
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 5 cut(s) 231, 291, 293, 297, 300
BspANI GGCC 6 cut(s) 296, 758, 1003, 1038, 1099, 1195
BspCNI CTCAG 4 cut(s) 563, 586, 701, 1144
BspFNI CGCG 2 cut(s) 293, 980
BspLI GGNNCC 4 cut(s) 289, 463, 547, 951
BspPI GGATC 1 cut(s) 785
BspQI GCTCTTC 1 cut(s) 794
BspT107I GGYRCC 3 cut(s) 287, 461, 545
BsrDI GCAATG 1 cut(s) 68
BsrI ACTGG 6 cut(s) 289, 482, 711, 978, 1024, 1253
BssECI CCNNGG 3 cut(s) 291, 579, 964
BssMI GATC 2 cut(s) 777, 939
BssNI GRCGYC 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 4 cut(s) 966, 1008, 1035, 1225
Bst4CI ACNGT 4 cut(s) 52, 208, 517, 640
Bst6I CTCTTC 2 cut(s) 231, 794
BstACI GRCGYC 1 cut(s) 462
BstDEI CTNAG 5 cut(s) 9, 550, 594, 688, 1131
BstDSI CCRYGG 2 cut(s) 291, 579
BstF5I GGATG 6 cut(s) 45, 181, 400, 622, 750, 928
BstFNI CGCG 2 cut(s) 293, 980
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 2 cut(s) 464, 483
BstKTI GATC 2 cut(s) 780, 942
BstMAI GTCTC 1 cut(s) 991
BstMBI GATC 2 cut(s) 777, 939
BstMCI CGRYCG 1 cut(s) 297
BstMWI GCNNNNNNNGC 5 cut(s) 91, 272, 296, 299, 977
BstNI CCWGG 4 cut(s) 966, 1008, 1035, 1225
BstNSI RCATGY 1 cut(s) 675
BstPAI GACNNNNGTC 1 cut(s) 192
BstSCI CCNGG 5 cut(s) 964, 1006, 1017, 1033, 1223
BstSFI CTRYAG 1 cut(s) 1242
BstSLI GKGCMC 2 cut(s) 973, 1176
BstUI CGCG 2 cut(s) 293, 980
BstV1I GCAGC 2 cut(s) 278, 771
BstV2I GAAGAC 1 cut(s) 1080
BstZI CGGCCG 1 cut(s) 294
BsuRI GGCC 6 cut(s) 296, 758, 1003, 1038, 1099, 1195
BtgI CCRYGG 2 cut(s) 291, 579
BtgZI GCGATG 1 cut(s) 1083
BtsCI GGATG 6 cut(s) 45, 181, 400, 622, 750, 928
BtsI GCAGTG 1 cut(s) 1174
BtsIMutI CAGTG 3 cut(s) 475, 971, 1174
CciNI GCGGCCGC 1 cut(s) 294
CfoI GCGC 2 cut(s) 464, 483
Cfr13I GGNCC 6 cut(s) 1001, 1010, 1021, 1097, 1194, 1204
Cfr42I CCGCGG 1 cut(s) 294
CseI GACGC 1 cut(s) 986
Csp6I GTAC 3 cut(s) 282, 349, 376
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 4 cut(s) 235, 352, 580, 672
CviQI GTAC 3 cut(s) 282, 349, 376
DdeI CTNAG 5 cut(s) 9, 550, 594, 688, 1131
DinI GGCGCC 1 cut(s) 463
DpnI GATC 2 cut(s) 779, 941
DpnII GATC 2 cut(s) 777, 939
DraI TTTAAA 1 cut(s) 729
DrdI GACNNNNNNGTC 1 cut(s) 983
DseDI GACNNNNNNGTC 1 cut(s) 983
EaeI YGGCCR 1 cut(s) 294
EagI CGGCCG 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 231, 794
EarI CTCTTC 2 cut(s) 231, 794
EclXI CGGCCG 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 579
Eco147I AGGCCT 1 cut(s) 1038
Eco47I GGWCC 3 cut(s) 1010, 1021, 1204
Eco52I CGGCCG 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 190
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 4 cut(s) 964, 1006, 1033, 1223
EcoT14I CCWWGG 1 cut(s) 579
EgeI GGCGCC 1 cut(s) 463
EheI GGCGCC 1 cut(s) 463
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 4 cut(s) 238, 355, 583, 675
FalI AAGNNNNNCTT 2 cut(s) 773, 805
FaqI GGGAC 1 cut(s) 717
FatI CATG 4 cut(s) 234, 351, 579, 671
FauI CCCGC 1 cut(s) 238
Fnu4HI GCNGC 6 cut(s) 267, 291, 294, 297, 300, 785
FokI GGATG 6 cut(s) 52, 168, 387, 629, 757, 935
Fsp4HI GCNGC 6 cut(s) 267, 291, 294, 297, 300, 785
FspBI CTAG 5 cut(s) 126, 258, 279, 719, 1055
GlaI GCGC 2 cut(s) 463, 482
GluI GCNGC 6 cut(s) 267, 291, 294, 297, 300, 785
GsaI CCCAGC 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 6 cut(s) 296, 758, 1003, 1038, 1099, 1195
HapII CCGG 1 cut(s) 1019
HgaI GACGC 1 cut(s) 986
HhaI GCGC 2 cut(s) 464, 483
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 4 cut(s) 238, 355, 583, 675
Hin6I GCGC 2 cut(s) 462, 481
HinP1I GCGC 2 cut(s) 462, 481
HinfI GANTC 6 cut(s) 159, 193, 421, 692, 914, 1051
HpaII CCGG 1 cut(s) 1019
HphI GGTGA 2 cut(s) 58, 188
Hpy166II GTNNAC 6 cut(s) 149, 451, 901, 971, 1083, 1207
Hpy188I TCNGA 6 cut(s) 25, 420, 553, 630, 691, 1134
Hpy188III TCNNGA 2 cut(s) 185, 404
Hpy8I GTNNAC 6 cut(s) 149, 451, 901, 971, 1083, 1207
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 3 cut(s) 361, 853, 1137
HpyCH4III ACNGT 4 cut(s) 52, 208, 517, 640
HpyCH4V TGCA 6 cut(s) 85, 266, 413, 524, 671, 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 272, 296, 299, 977
HpyF3I CTNAG 5 cut(s) 9, 550, 594, 688, 1131
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 4 cut(s) 238, 355, 583, 675
HspAI GCGC 2 cut(s) 462, 481
KasI GGCGCC 1 cut(s) 461
KspI CCGCGG 1 cut(s) 294
Kzo9I GATC 2 cut(s) 777, 939
LguI GCTCTTC 1 cut(s) 794
LmnI GCTCC 2 cut(s) 325, 436
Lsp1109I GCAGC 2 cut(s) 278, 771
LweI GCATC 1 cut(s) 400
MaeI CTAG 5 cut(s) 126, 258, 279, 719, 1055
MaeIII GTNAC 4 cut(s) 46, 194, 959, 994
MalI GATC 2 cut(s) 779, 941
MboI GATC 2 cut(s) 777, 939
MboII GAAGA 5 cut(s) 218, 419, 434, 781, 1085
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 2 cut(s) 973, 1176
MluCI AATT 6 cut(s) 19, 320, 525, 730, 807, 929
MluI ACGCGT 1 cut(s) 978
Mly113I GGCGCC 1 cut(s) 462
MlyI GAGTC 2 cut(s) 168, 202
MmeI TCCRAC 2 cut(s) 297, 1209
MroXI GAANNNNTTC 1 cut(s) 624
MseI TTAA 8 cut(s) 210, 243, 339, 492, 599, 728, 878, 1028
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 1019
MspR9I CCNGG 5 cut(s) 966, 1008, 1019, 1035, 1225
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 4 cut(s) 966, 1008, 1035, 1225
MvnI CGCG 2 cut(s) 293, 980
MwoI GCNNNNNNNGC 5 cut(s) 91, 272, 296, 299, 977
NarI GGCGCC 1 cut(s) 462
NciI CCSGG 1 cut(s) 1019
NcoI CCATGG 1 cut(s) 579
NdeII GATC 2 cut(s) 777, 939
NlaIII CATG 4 cut(s) 238, 355, 583, 675
NlaIV GGNNCC 4 cut(s) 289, 463, 547, 951
NmuCI GTSAC 3 cut(s) 46, 194, 959
NotI GCGGCCGC 1 cut(s) 294
NspI RCATGY 1 cut(s) 675
PceI AGGCCT 1 cut(s) 1038
PciSI GCTCTTC 1 cut(s) 794
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 624
PfeI GAWTC 4 cut(s) 421, 692, 914, 1051
PflMI CCANNNNNTGG 2 cut(s) 40, 1094
PkrI GCNGC 6 cut(s) 268, 292, 295, 298, 301, 786
PleI GAGTC 2 cut(s) 167, 201
PluTI GGCGCC 1 cut(s) 465
PpsI GAGTC 2 cut(s) 167, 201
PshAI GACNNNNGTC 1 cut(s) 192
Psp6I CCWGG 4 cut(s) 964, 1006, 1033, 1223
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 4 cut(s) 964, 1006, 1033, 1223
PspN4I GGNNCC 4 cut(s) 289, 463, 547, 951
PspPI GGNCC 6 cut(s) 1001, 1010, 1021, 1097, 1194, 1204
RsaI GTAC 3 cut(s) 283, 350, 377
RsaNI GTAC 3 cut(s) 282, 349, 376
SacII CCGCGG 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 794
SaqAI TTAA 8 cut(s) 210, 243, 339, 492, 599, 728, 878, 1028
SatI GCNGC 6 cut(s) 267, 291, 294, 297, 300, 785
Sau3AI GATC 2 cut(s) 777, 939
Sau96I GGNCC 6 cut(s) 1001, 1010, 1021, 1097, 1194, 1204
ScaI AGTACT 1 cut(s) 283
SchI GAGTC 2 cut(s) 168, 202
ScrFI CCNGG 5 cut(s) 966, 1008, 1019, 1035, 1225
SduI GDGCHC 2 cut(s) 973, 1176
SetI ASST 6 cut(s) 330, 534, 786, 835, 970, 1129
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 1 cut(s) 1242
SfoI GGCGCC 1 cut(s) 463
Sfr303I CCGCGG 1 cut(s) 294
SgrBI CCGCGG 1 cut(s) 294
SinI GGWCC 3 cut(s) 1010, 1021, 1204
SpeI ACTAGT 2 cut(s) 125, 278
Sse9I AATT 6 cut(s) 19, 320, 525, 730, 807, 929
SseBI AGGCCT 1 cut(s) 1038
SsiI CCGC 5 cut(s) 231, 291, 293, 297, 300
SspDI GGCGCC 1 cut(s) 461
SspI AATATT 2 cut(s) 80, 490
SspMI CTAG 5 cut(s) 126, 258, 279, 719, 1055
StuI AGGCCT 1 cut(s) 1038
StyD4I CCNGG 5 cut(s) 964, 1006, 1017, 1033, 1223
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 4 cut(s) 52, 208, 517, 640
TaqI TCGA 4 cut(s) 98, 712, 1092, 1139
TasI AATT 6 cut(s) 19, 320, 525, 730, 807, 929
TatI WGTACW 2 cut(s) 281, 348
TauI GCSGC 4 cut(s) 293, 296, 299, 302
TfiI GAWTC 4 cut(s) 421, 692, 914, 1051
Tru1I TTAA 8 cut(s) 210, 243, 339, 492, 599, 728, 878, 1028
Tru9I TTAA 8 cut(s) 210, 243, 339, 492, 599, 728, 878, 1028
TscAI CASTG 3 cut(s) 482, 978, 1181
TseFI GTSAC 3 cut(s) 46, 194, 959
TseI GCWGC 2 cut(s) 266, 784
Tsp45I GTSAC 3 cut(s) 46, 194, 959
TspDTI ATGAA 8 cut(s) 57, 251, 368, 561, 633, 761, 835, 1086
TspGWI ACGGA 1 cut(s) 457
TspRI CASTG 3 cut(s) 482, 978, 1181
Van91I CCANNNNNTGG 2 cut(s) 40, 1094
VneI GTGCAC 1 cut(s) 969
VpaK11BI GGWCC 3 cut(s) 1010, 1021, 1204
XapI RAATTY 1 cut(s) 19
XceI RCATGY 1 cut(s) 675
XmnI GAANNNNTTC 1 cut(s) 624
XspI CTAG 5 cut(s) 126, 258, 279, 719, 1055
ZrmI AGTACT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.