Rw6G038640

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015325
Physical Location & Seq
Reverse (-)
62707793 .. 62710039
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G038640.1

Sequence Viewer

Length: 1215 bp
ATGGGGGTGACGGTAATATCTCCCCATTCATTGCCACAATATTTGCATTTACAGCAGTCGATACCAAACCAGAAAGCAGTAAAAACTAGTTGTTTGCTTAGCTATGGTAGACGAGGAGGAGTCTTGCTTGGTCAAAACAACCATCCAGACCCGACCACCGTTAATAGTGTCACTACTGTTCCCGCCATGAAACTTAACTCATCATCATCTAGTATTGCAGCAGAGGCAACTAGTACTGGTGCCGCGGCCGCCGCCGACGATGTAGTGTTGGAATTGGAGCTGGGAGAGATTAAGGACAAGTACGTGAAGTGGAAGTGGAAGGGTCAGTACGCCATCAACTACATTGTTTCACATCCAGAAGATGCAAATCCGAATCCAAATGCTCCTCCTATTCTTCTTGTTCACGGCTTCGGCGCCTCCGTTCCTCACTGGCGCAGAAATATTAAGACATTGGCGAAGCATTATACAGTTTATGCTATTGACCTGATTGGCTTTGGTGCCTCAGATAAGCCGATAGGCTTTTCATACACCATGGAGAAATGGGCTGAGTTAATCTTGGATTTTTTGGATGAAGTTGTTCAGAAGCCTACTGTTTTAATAGGCAACTCTGTGGGAAGCCTTGCATGTGTAATCTCTGCCTCAGAATCTAGAAAGGGACTGGTTCGAGGGCTAGTGCTTTTGAATTGCTCTGGTGGGATGAATAACAAGGCCATTGTTGATGATTGGAGGATCAAGCTGCTTTTCCCTTTGCTCATGCTAATTGATTTCTTGTTGAAGCAACGACCTATTGCTTCCTTCATTTTTGAGCGTGTTAGACAGAGGGAAAGTTTAAGAAATATCTTATCATCTGTTTACGCCAACAAAGAATCTGTGGATGACGAATTAATAGAGATCATAATGGAACCAACAAGTGACCCAGGTGCACTGGACGCTTTTGTCTCAATCGTAACAGGCCCACCAGGACCAAACCCGGTCCAGTTAATGCCAGGCCTCTCCCTACCGATTCTAGTCCTATGGGGAGATGAAGACCCGTTCACTCCACTCGATGGGCCTGTTGGCAAATACTTCACTTCCTTACCTTCTCAGAAATCGAATGTGAGCCTCTCCATTTTACAAGGAGTTGGGCACTGCCCCCACGATGACAGGCCTATGTTGGTCCACGACAAACTTCTTCCCTGGTTGGATAATCTTTCTATAGAAAATCCAGTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

404

Amino Acids

44.09

Weight (kDa)

5.94

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 129 - 383 1.2e-25 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 131 - 383 1.4e-20 Alpha/beta hydrolase family
Hydrolase_4 PF12146 131 - 239 7.1e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38520 AT5G38520
fragaria_vesca FvH4_2g37190
malus_domestica MD08G1001000.v1.1
prunus_persica Prupe.1G353100_v2.0.a1
pyrus_communis pycom08g00040
rosa_chinensis RchiOBHm_Chr6g0304501
rosa_laevigata RLG00000010971
rosa_multiflora Rmu_sc0023263.1_g000001 Rmu_ssc0000218.1_g000058
rosa_roxburghii Rroxscaffold_7G00163690
rosa_rugosa Rorug06G0332600
rosa_samantha Rh6AG445100 Rh6CG457900 Rh6DG444700
rosa_wichuraiana Rw6G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 935
AccB1I GGYRCC 3 cut(s) 239, 413, 497
AccB7I CCANNNNNTGG 1 cut(s) 1046
AccI GTMKAC 1 cut(s) 109
AccII CGCG 1 cut(s) 245
AciI CCGC 5 cut(s) 183, 243, 245, 249, 252
AclWI GGATC 1 cut(s) 737
AcoI YGGCCR 1 cut(s) 246
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 3 cut(s) 235, 302, 329
AfiI CCNNNNNNNGG 1 cut(s) 1046
AgsI TTSAA 2 cut(s) 682, 775
AhlI ACTAGT 2 cut(s) 86, 230
AjnI CCWGG 4 cut(s) 916, 958, 985, 1175
AluBI AGCT 3 cut(s) 102, 280, 736
AluI AGCT 3 cut(s) 102, 280, 736
Alw21I GWGCWC 1 cut(s) 925
Alw26I GTCTC 1 cut(s) 943
Alw44I GTGCAC 1 cut(s) 921
AlwI GGATC 1 cut(s) 737
AoxI GGCC 6 cut(s) 246, 708, 952, 988, 1049, 1145
ApaLI GTGCAC 1 cut(s) 921
ApeKI GCWGC 2 cut(s) 218, 736
AseI ATTAAT 1 cut(s) 884
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 2 cut(s) 416, 435
AspS9I GGNCC 5 cut(s) 953, 962, 973, 1049, 1156
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 3 cut(s) 962, 973, 1156
BaeGI GKGCMC 2 cut(s) 925, 1128
BanI GGYRCC 3 cut(s) 239, 413, 497
BarI GAAGNNNNNNTAC 2 cut(s) 311, 343
BbsI GAAGAC 1 cut(s) 1032
Bbv12I GWGCWC 1 cut(s) 925
BbvI GCAGC 2 cut(s) 230, 723
BccI CCATC 3 cut(s) 150, 341, 1040
BceAI ACGGC 1 cut(s) 421
BciT130I CCWGG 4 cut(s) 918, 960, 987, 1177
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 1 cut(s) 943
BcuI ACTAGT 2 cut(s) 86, 230
BfaI CTAG 6 cut(s) 87, 210, 231, 648, 671, 1007
BfmI CTRYAG 1 cut(s) 1194
BfoI RGCGCY 1 cut(s) 417
BisI GCNGC 6 cut(s) 219, 243, 246, 249, 252, 737
BlpI GCTNAGC 1 cut(s) 98
BlsI GCNGC 6 cut(s) 220, 244, 247, 250, 253, 738
BmcAI AGTACT 1 cut(s) 235
Bme1390I CCNGG 5 cut(s) 918, 960, 971, 987, 1177
Bme18I GGWCC 3 cut(s) 962, 973, 1156
BmgT120I GGNCC 5 cut(s) 953, 962, 973, 1049, 1156
BmiI GGNNCC 4 cut(s) 241, 415, 499, 903
BmrFI CCNGG 5 cut(s) 918, 960, 971, 987, 1177
BmsI GCATC 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 1032
Bpu1102I GCTNAGC 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 971
BsaAI YACGTR 1 cut(s) 304
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 414
BsaJI CCNNGG 4 cut(s) 243, 531, 916, 1175
Bsc4I CCNNNNNNNGG 1 cut(s) 1046
Bse1I ACTGG 6 cut(s) 241, 434, 663, 930, 976, 1205
Bse3DI GCAATG 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 4 cut(s) 918, 960, 987, 1177
BseDI CCNNGG 4 cut(s) 243, 531, 916, 1175
BseGI GGATG 5 cut(s) 142, 352, 574, 702, 880
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 1046
BseMI GCAATG 1 cut(s) 29
BseMII CTCAG 4 cut(s) 516, 537, 654, 1097
BseNI ACTGG 6 cut(s) 241, 434, 663, 930, 976, 1205
BseRI GAGGAG 3 cut(s) 129, 132, 375
BseSI GKGCMC 2 cut(s) 925, 1128
BseX3I CGGCCG 1 cut(s) 246
BseXI GCAGC 2 cut(s) 230, 723
BseYI CCCAGC 1 cut(s) 280
Bsh1236I CGCG 1 cut(s) 245
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 6 cut(s) 248, 710, 954, 990, 1051, 1147
BshNI GGYRCC 3 cut(s) 239, 413, 497
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 925
BsiSI CCGG 1 cut(s) 971
BslFI GGGAC 1 cut(s) 669
BslI CCNNNNNNNGG 1 cut(s) 1046
BsmAI GTCTC 1 cut(s) 943
BsmFI GGGAC 1 cut(s) 669
BsnI GGCC 6 cut(s) 248, 710, 954, 990, 1051, 1147
Bsp1286I GDGCHC 2 cut(s) 925, 1128
Bsp143I GATC 2 cut(s) 729, 891
Bsp1720I GCTNAGC 1 cut(s) 98
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 5 cut(s) 183, 243, 245, 249, 252
BspANI GGCC 6 cut(s) 248, 710, 954, 990, 1051, 1147
BspCNI CTCAG 4 cut(s) 515, 538, 653, 1096
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 4 cut(s) 241, 415, 499, 903
BspPI GGATC 1 cut(s) 737
BspT107I GGYRCC 3 cut(s) 239, 413, 497
BsrDI GCAATG 1 cut(s) 29
BsrI ACTGG 6 cut(s) 241, 434, 663, 930, 976, 1205
BssECI CCNNGG 4 cut(s) 243, 531, 916, 1175
BssMI GATC 2 cut(s) 729, 891
BssNI GRCGYC 1 cut(s) 414
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 4 cut(s) 918, 960, 987, 1177
Bst4CI ACNGT 5 cut(s) 13, 160, 178, 469, 592
BstACI GRCGYC 1 cut(s) 414
BstBAI YACGTR 1 cut(s) 304
BstDEI CTNAG 5 cut(s) 98, 502, 546, 640, 1083
BstDSI CCRYGG 2 cut(s) 243, 531
BstF5I GGATG 5 cut(s) 142, 352, 574, 702, 880
BstFNI CGCG 1 cut(s) 245
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 2 cut(s) 416, 435
BstKTI GATC 2 cut(s) 732, 894
BstMAI GTCTC 1 cut(s) 943
BstMBI GATC 2 cut(s) 729, 891
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 5 cut(s) 52, 224, 248, 251, 929
BstNI CCWGG 4 cut(s) 918, 960, 987, 1177
BstNSI RCATGY 1 cut(s) 627
BstSCI CCNGG 5 cut(s) 916, 958, 969, 985, 1175
BstSFI CTRYAG 1 cut(s) 1194
BstSLI GKGCMC 2 cut(s) 925, 1128
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 2 cut(s) 230, 723
BstV2I GAAGAC 1 cut(s) 1032
BstZI CGGCCG 1 cut(s) 246
BsuRI GGCC 6 cut(s) 248, 710, 954, 990, 1051, 1147
BtgI CCRYGG 2 cut(s) 243, 531
BtsCI GGATG 5 cut(s) 142, 352, 574, 702, 880
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 3 cut(s) 427, 923, 1126
CciNI GCGGCCGC 1 cut(s) 246
CfoI GCGC 2 cut(s) 416, 435
Cfr13I GGNCC 5 cut(s) 953, 962, 973, 1049, 1156
Cfr42I CCGCGG 1 cut(s) 246
CseI GACGC 1 cut(s) 938
Csp6I GTAC 3 cut(s) 234, 301, 328
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviAII CATG 4 cut(s) 187, 532, 624, 754
CviQI GTAC 3 cut(s) 234, 301, 328
DdeI CTNAG 5 cut(s) 98, 502, 546, 640, 1083
DinI GGCGCC 1 cut(s) 415
DpnI GATC 2 cut(s) 731, 893
DpnII GATC 2 cut(s) 729, 891
DrdI GACNNNNNNGTC 1 cut(s) 935
DseDI GACNNNNNNGTC 1 cut(s) 935
EaeI YGGCCR 1 cut(s) 246
EagI CGGCCG 1 cut(s) 246
EclXI CGGCCG 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 531
Eco147I AGGCCT 2 cut(s) 990, 1147
Eco47I GGWCC 3 cut(s) 962, 973, 1156
Eco52I CGGCCG 1 cut(s) 246
EcoRII CCWGG 4 cut(s) 916, 958, 985, 1175
EcoT14I CCWWGG 1 cut(s) 531
EgeI GGCGCC 1 cut(s) 415
EheI GGCGCC 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 4 cut(s) 190, 535, 627, 757
FaqI GGGAC 1 cut(s) 669
FatI CATG 4 cut(s) 186, 531, 623, 753
FauI CCCGC 1 cut(s) 190
FblI GTMKAC 1 cut(s) 109
Fnu4HI GCNGC 6 cut(s) 219, 243, 246, 249, 252, 737
FokI GGATG 5 cut(s) 129, 339, 581, 709, 887
Fsp4HI GCNGC 6 cut(s) 219, 243, 246, 249, 252, 737
FspBI CTAG 6 cut(s) 87, 210, 231, 648, 671, 1007
GlaI GCGC 2 cut(s) 415, 434
GluI GCNGC 6 cut(s) 219, 243, 246, 249, 252, 737
GsaI CCCAGC 1 cut(s) 284
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 6 cut(s) 248, 710, 954, 990, 1051, 1147
HapII CCGG 1 cut(s) 971
HgaI GACGC 1 cut(s) 938
HhaI GCGC 2 cut(s) 416, 435
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 4 cut(s) 190, 535, 627, 757
Hin6I GCGC 2 cut(s) 414, 433
HinP1I GCGC 2 cut(s) 414, 433
HinfI GANTC 5 cut(s) 120, 373, 644, 866, 1003
HpaII CCGG 1 cut(s) 971
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 6 cut(s) 110, 403, 853, 923, 1035, 1159
Hpy188I TCNGA 5 cut(s) 372, 505, 582, 643, 1086
Hpy188III TCNNGA 3 cut(s) 146, 356, 648
Hpy8I GTNNAC 6 cut(s) 110, 403, 853, 923, 1035, 1159
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 313, 805, 1089
HpyCH4III ACNGT 5 cut(s) 13, 160, 178, 469, 592
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 5 cut(s) 46, 218, 365, 623, 923
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 224, 248, 251, 929
HpyF3I CTNAG 5 cut(s) 98, 502, 546, 640, 1083
HpySE526I ACGT 1 cut(s) 303
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 4 cut(s) 190, 535, 627, 757
HspAI GCGC 2 cut(s) 414, 433
KasI GGCGCC 1 cut(s) 413
KspI CCGCGG 1 cut(s) 246
Kzo9I GATC 2 cut(s) 729, 891
LmnI GCTCC 2 cut(s) 277, 388
Lsp1109I GCAGC 2 cut(s) 230, 723
LweI GCATC 1 cut(s) 352
MaeI CTAG 6 cut(s) 87, 210, 231, 648, 671, 1007
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 4 cut(s) 7, 169, 911, 946
MalI GATC 2 cut(s) 731, 893
MboI GATC 2 cut(s) 729, 891
MboII GAAGA 4 cut(s) 371, 386, 1037, 1163
MhlI GDGCHC 2 cut(s) 925, 1128
MluCI AATT 4 cut(s) 272, 682, 759, 881
Mly113I GGCGCC 1 cut(s) 414
MlyI GAGTC 1 cut(s) 129
MmeI TCCRAC 2 cut(s) 249, 1161
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 9 cut(s) 162, 195, 291, 444, 551, 596, 830, 884, 980
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 1 cut(s) 971
MspR9I CCNGG 5 cut(s) 918, 960, 971, 987, 1177
MvaI CCWGG 4 cut(s) 918, 960, 987, 1177
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 5 cut(s) 52, 224, 248, 251, 929
NarI GGCGCC 1 cut(s) 414
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 1 cut(s) 531
NdeII GATC 2 cut(s) 729, 891
NlaIII CATG 4 cut(s) 190, 535, 627, 757
NlaIV GGNNCC 4 cut(s) 241, 415, 499, 903
NmuCI GTSAC 3 cut(s) 7, 169, 911
NotI GCGGCCGC 1 cut(s) 246
NspI RCATGY 1 cut(s) 627
PceI AGGCCT 2 cut(s) 990, 1147
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 4 cut(s) 373, 644, 866, 1003
PflMI CCANNNNNTGG 1 cut(s) 1046
PkrI GCNGC 6 cut(s) 220, 244, 247, 250, 253, 738
PleI GAGTC 1 cut(s) 128
PluTI GGCGCC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 128
Ppu21I YACGTR 1 cut(s) 304
PshBI ATTAAT 1 cut(s) 884
Psp6I CCWGG 4 cut(s) 916, 958, 985, 1175
PspFI CCCAGC 1 cut(s) 280
PspGI CCWGG 4 cut(s) 916, 958, 985, 1175
PspN4I GGNNCC 4 cut(s) 241, 415, 499, 903
PspPI GGNCC 5 cut(s) 953, 962, 973, 1049, 1156
RsaI GTAC 3 cut(s) 235, 302, 329
RsaNI GTAC 3 cut(s) 234, 301, 328
SacII CCGCGG 1 cut(s) 246
SaqAI TTAA 9 cut(s) 162, 195, 291, 444, 551, 596, 830, 884, 980
SatI GCNGC 6 cut(s) 219, 243, 246, 249, 252, 737
Sau3AI GATC 2 cut(s) 729, 891
Sau96I GGNCC 5 cut(s) 953, 962, 973, 1049, 1156
ScaI AGTACT 1 cut(s) 235
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 5 cut(s) 918, 960, 971, 987, 1177
SduI GDGCHC 2 cut(s) 925, 1128
SetI ASST 8 cut(s) 104, 282, 306, 486, 738, 787, 922, 1081
SfaNI GCATC 1 cut(s) 352
SfcI CTRYAG 1 cut(s) 1194
SfoI GGCGCC 1 cut(s) 415
Sfr303I CCGCGG 1 cut(s) 246
SgrBI CCGCGG 1 cut(s) 246
SinI GGWCC 3 cut(s) 962, 973, 1156
SpeI ACTAGT 2 cut(s) 86, 230
Sse9I AATT 4 cut(s) 272, 682, 759, 881
SseBI AGGCCT 2 cut(s) 990, 1147
SsiI CCGC 5 cut(s) 183, 243, 245, 249, 252
SspDI GGCGCC 1 cut(s) 413
SspI AATATT 2 cut(s) 41, 442
SspMI CTAG 6 cut(s) 87, 210, 231, 648, 671, 1007
StuI AGGCCT 2 cut(s) 990, 1147
StyD4I CCNGG 5 cut(s) 916, 958, 969, 985, 1175
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 5 cut(s) 13, 160, 178, 469, 592
TaiI ACGT 1 cut(s) 306
TaqI TCGA 4 cut(s) 59, 664, 1044, 1091
TasI AATT 4 cut(s) 272, 682, 759, 881
TatI WGTACW 1 cut(s) 233
TauI GCSGC 4 cut(s) 245, 248, 251, 254
TfiI GAWTC 4 cut(s) 373, 644, 866, 1003
Tru1I TTAA 9 cut(s) 162, 195, 291, 444, 551, 596, 830, 884, 980
Tru9I TTAA 9 cut(s) 162, 195, 291, 444, 551, 596, 830, 884, 980
TscAI CASTG 3 cut(s) 434, 930, 1133
TseFI GTSAC 3 cut(s) 7, 169, 911
TseI GCWGC 2 cut(s) 218, 736
Tsp45I GTSAC 3 cut(s) 7, 169, 911
TspDTI ATGAA 7 cut(s) 18, 203, 513, 585, 713, 787, 1038
TspGWI ACGGA 1 cut(s) 409
TspRI CASTG 3 cut(s) 434, 930, 1133
Van91I CCANNNNNTGG 1 cut(s) 1046
VneI GTGCAC 1 cut(s) 921
VpaK11BI GGWCC 3 cut(s) 962, 973, 1156
VspI ATTAAT 1 cut(s) 884
XbaI TCTAGA 1 cut(s) 647
XceI RCATGY 1 cut(s) 627
XmiI GTMKAC 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 6 cut(s) 87, 210, 231, 648, 671, 1007
ZrmI AGTACT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.