AT5G38660

Protein of unknown function (DUF2854)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009598
Physical Location & Seq
Reverse (-)
15470900 .. 15476553
5654 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G38660.1

Sequence Viewer

Length: 861 bp
ATGGGATCTATAACGGTAGCTCCGGGAACCACCGTCTTATTCTCCAGTCGGCGGCTAGATCTTTGCGGTCGTCGCCATGAGAATCTATCCGTCGTTTCGTCCTCACAACCGTCTCTGATGCCAAAGCAGAGATGGCTTTATATTCCCGAGACGAGGTTGAAAAGAGAGGTTTTAAAGCTCGATGTCGTCGGAAGAGCTGCTGATTCCACGAGCTCTTCTCCATCAGTAGCTTCCGGTGATAGAACCTTAATTCCTGATGATGAATTCACTTTAGCCAAGATTTCATTTGGTGTTATTGGGCTAGGTCTCGGGGTTTCGCTACTCTCGTATGGTTTTGGGGCCTATTTTACTATCCTTCCTGGAACTGAGTGGTCTGCTATAATGCTAACATATGGATTTCCCCTTTCTATTATCGGAATGGCTCTTAAGTACGCAGAACTCAAACCAGTTCCTTGCTTGAGCTATTCGGATGCGGTGAAGCTTAGAGAAAGCTGTGCTACTCCTATTTTAACGCAGGTTAGAAATGATGTCACGAGATACCGTTATGGAGATGAACAACATTTGGAAGAAGCACTAAAACGAATCTTTCAATATGGGCTGGGAGGAGGAATCCCGAGACGTAGTGCACCTATCTTACAGCTGATAAGGGAAGAGGTCCTGACCGATGGTCGGTACTGTGTGGTCCTTGTATTTGAGGCCAAAGCTCTGACGTTGTCAGATTTTGAAAAAAGACAGGCGAAATTCACTTCCTTCTTTGGACCAAACATCACGGCAGAAGTCGGTAAAGGAGAAAGTGAGAATCTCTATGAAGTAAGACTGATTTCCAACCTCTCCACCAACTCTGTATCTTCTACCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2854

Protein Analysis

286

Amino Acids

31.44

Weight (kDa)

8.6

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2854 PF11016 110 - 260 2.7e-46 Protein of unknown function (DUF2854)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 505
AciI CCGC 3 cut(s) 52, 66, 473
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 2 cut(s) 263, 740
AfaI GTAC 2 cut(s) 431, 674
AfiI CCNNNNNNNGG 3 cut(s) 51, 153, 669
AflII CTTAAG 1 cut(s) 425
AgsI TTSAA 3 cut(s) 160, 590, 725
AhdI GACNNNNNGTC 1 cut(s) 666
AjnI CCWGG 1 cut(s) 358
AloI GAACNNNNNNTCC 2 cut(s) 235, 267
Alw21I GWGCWC 2 cut(s) 215, 628
Alw26I GTCTC 4 cut(s) 117, 143, 311, 610
Alw44I GTGCAC 1 cut(s) 624
AlwI GGATC 1 cut(s) 13
Ama87I CYCGRG 3 cut(s) 146, 308, 613
AoxI GGCC 2 cut(s) 339, 696
ApaLI GTGCAC 1 cut(s) 624
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 2 cut(s) 263, 740
AspS9I GGNCC 4 cut(s) 339, 655, 682, 758
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 248, 487
AvaI CYCGRG 3 cut(s) 146, 308, 613
AvaII GGWCC 3 cut(s) 655, 682, 758
BaeGI GKGCMC 1 cut(s) 628
BanII GRGCYC 1 cut(s) 215
BauI CACGAG 2 cut(s) 208, 532
Bbv12I GWGCWC 2 cut(s) 215, 628
BbvI GCAGC 1 cut(s) 184
BccI CCATC 3 cut(s) 126, 229, 659
BceAI ACGGC 1 cut(s) 786
BciT130I CCWGG 1 cut(s) 360
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 4 cut(s) 117, 143, 311, 610
BfaI CTAG 2 cut(s) 56, 302
BfrI CTTAAG 1 cut(s) 425
BfuAI ACCTGC 1 cut(s) 505
BglII AGATCT 1 cut(s) 58
BisI GCNGC 2 cut(s) 53, 198
BlsI GCNGC 2 cut(s) 54, 199
Bme1390I CCNGG 2 cut(s) 24, 360
Bme18I GGWCC 3 cut(s) 655, 682, 758
BmeRI GACNNNNNGTC 1 cut(s) 666
BmeT110I CYCGRG 3 cut(s) 146, 308, 613
BmgT120I GGNCC 4 cut(s) 339, 655, 682, 758
BmiI GGNNCC 2 cut(s) 28, 340
BmrFI CCNGG 2 cut(s) 24, 360
BmsI GCATC 2 cut(s) 108, 460
BplI GAGNNNNNCTC 2 cut(s) 202, 234
BpmI CTGGAG 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 478
BpuMI CCSGG 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 311
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 153, 669
Bse1I ACTGG 2 cut(s) 45, 446
BseBI CCWGG 1 cut(s) 360
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 3 cut(s) 51, 153, 669
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 45, 446
BseRI GAGGAG 1 cut(s) 618
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 1 cut(s) 184
BseYI CCCAGC 1 cut(s) 598
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 2 cut(s) 341, 698
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 2 cut(s) 215, 628
BsiHKCI CYCGRG 3 cut(s) 146, 308, 613
BsiSI CCGG 2 cut(s) 23, 234
BslI CCNNNNNNNGG 3 cut(s) 51, 153, 669
BsmAI GTCTC 4 cut(s) 117, 143, 311, 610
BsmBI CGTCTC 3 cut(s) 117, 143, 610
BsnI GGCC 2 cut(s) 341, 698
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 3 cut(s) 146, 308, 613
Bsp1286I GDGCHC 2 cut(s) 215, 628
Bsp143I GATC 2 cut(s) 5, 58
BspACI CCGC 3 cut(s) 52, 66, 473
BspANI GGCC 2 cut(s) 341, 698
BspCNI CTCAG 1 cut(s) 358
BspLI GGNNCC 2 cut(s) 28, 340
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 1 cut(s) 13
BspQI GCTCTTC 2 cut(s) 187, 220
BspTI CTTAAG 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 311
BsrI ACTGG 2 cut(s) 45, 446
BssMI GATC 2 cut(s) 5, 58
BssSI CACGAG 2 cut(s) 208, 532
Bst2BI CACGAG 2 cut(s) 208, 532
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 5 cut(s) 16, 34, 111, 542, 677
Bst6I CTCTTC 3 cut(s) 187, 220, 645
BstAFI CTTAAG 1 cut(s) 425
BstDEI CTNAG 2 cut(s) 366, 482
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 2 cut(s) 8, 61
BstMAI GTCTC 4 cut(s) 117, 143, 311, 610
BstMBI GATC 2 cut(s) 5, 58
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 2 cut(s) 72, 133
BstNI CCWGG 1 cut(s) 360
BstSCI CCNGG 2 cut(s) 22, 358
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 1 cut(s) 184
BstX2I RGATCY 2 cut(s) 5, 58
BstYI RGATCY 2 cut(s) 5, 58
BsuRI GGCC 2 cut(s) 341, 698
BtsCI GGATG 1 cut(s) 475
BveI ACCTGC 1 cut(s) 505
Cfr13I GGNCC 4 cut(s) 339, 655, 682, 758
Csp6I GTAC 2 cut(s) 430, 673
CviAII CATG 1 cut(s) 77
CviQI GTAC 2 cut(s) 430, 673
DdeI CTNAG 2 cut(s) 366, 482
DpnI GATC 2 cut(s) 7, 60
DpnII GATC 2 cut(s) 5, 58
DraI TTTAAA 1 cut(s) 174
DriI GACNNNNNGTC 1 cut(s) 666
Eam1104I CTCTTC 3 cut(s) 187, 220, 645
Eam1105I GACNNNNNGTC 1 cut(s) 666
EarI CTCTTC 3 cut(s) 187, 220, 645
Ecl136II GAGCTC 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 311
Eco47I GGWCC 3 cut(s) 655, 682, 758
Eco53kI GAGCTC 1 cut(s) 213
Eco88I CYCGRG 3 cut(s) 146, 308, 613
EcoICRI GAGCTC 1 cut(s) 213
EcoO109I RGGNCCY 2 cut(s) 339, 655
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 358
EcoT38I GRGCYC 1 cut(s) 215
Esp3I CGTCTC 3 cut(s) 117, 143, 610
FaeI CATG 1 cut(s) 80
FatI CATG 1 cut(s) 76
FauNDI CATATG 1 cut(s) 391
Fnu4HI GCNGC 2 cut(s) 53, 198
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 53, 198
FspBI CTAG 2 cut(s) 56, 302
GluI GCNGC 2 cut(s) 53, 198
GsaI CCCAGC 1 cut(s) 602
GsuI CTGGAG 1 cut(s) 28
HaeIII GGCC 2 cut(s) 341, 698
HapII CCGG 2 cut(s) 23, 234
Hin1II CATG 1 cut(s) 80
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 5 cut(s) 82, 203, 582, 609, 799
HpaII CCGG 2 cut(s) 23, 234
HphI GGTGA 2 cut(s) 248, 487
Hpy166II GTNNAC 1 cut(s) 626
Hpy188I TCNGA 6 cut(s) 117, 191, 416, 469, 708, 718
Hpy188III TCNNGA 6 cut(s) 146, 254, 532, 613, 658, 858
Hpy8I GTNNAC 1 cut(s) 626
Hpy99I CGWCG 3 cut(s) 75, 95, 191
HpyAV CCTTC 2 cut(s) 365, 760
HpyCH4III ACNGT 5 cut(s) 16, 34, 111, 542, 677
HpyCH4IV ACGT 2 cut(s) 619, 710
HpyCH4V TGCA 1 cut(s) 626
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 133
HpyF3I CTNAG 2 cut(s) 366, 482
HpySE526I ACGT 2 cut(s) 619, 710
Hsp92II CATG 1 cut(s) 80
Kzo9I GATC 2 cut(s) 5, 58
LguI GCTCTTC 2 cut(s) 187, 220
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 2 cut(s) 108, 460
MaeI CTAG 2 cut(s) 56, 302
MaeII ACGT 2 cut(s) 619, 710
MaeIII GTNAC 1 cut(s) 529
MalI GATC 2 cut(s) 7, 60
MboI GATC 2 cut(s) 5, 58
MboII GAAGA 5 cut(s) 204, 207, 578, 662, 840
MflI RGATCY 2 cut(s) 5, 58
MhlI GDGCHC 2 cut(s) 215, 628
MluCI AATT 3 cut(s) 249, 263, 740
MmeI TCCRAC 2 cut(s) 169, 849
MnlI CCTC 8 cut(s) 112, 147, 160, 596, 599, 646, 688, 839
MseI TTAA 4 cut(s) 173, 248, 426, 509
MspA1I CMGCKG 1 cut(s) 640
MspCI CTTAAG 1 cut(s) 425
MspI CCGG 2 cut(s) 23, 234
MspR9I CCNGG 2 cut(s) 24, 360
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 2 cut(s) 72, 133
NciI CCSGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 391
NdeII GATC 2 cut(s) 5, 58
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 2 cut(s) 28, 340
NmuCI GTSAC 1 cut(s) 529
PciSI GCTCTTC 2 cut(s) 187, 220
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PfeI GAWTC 5 cut(s) 82, 203, 582, 609, 799
PflFI GACNNNGTC 1 cut(s) 712
PfoI TCCNGGA 2 cut(s) 22, 358
PkrI GCNGC 2 cut(s) 54, 199
PpuMI RGGWCCY 1 cut(s) 655
Psp124BI GAGCTC 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 655
Psp6I CCWGG 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 598
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 2 cut(s) 28, 340
PspPI GGNCC 4 cut(s) 339, 655, 682, 758
PspPPI RGGWCCY 1 cut(s) 655
PsuI RGATCY 2 cut(s) 5, 58
PsyI GACNNNGTC 1 cut(s) 712
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 2 cut(s) 431, 674
RsaNI GTAC 2 cut(s) 430, 673
SacI GAGCTC 1 cut(s) 215
SapI GCTCTTC 2 cut(s) 187, 220
SaqAI TTAA 4 cut(s) 173, 248, 426, 509
SatI GCNGC 2 cut(s) 53, 198
Sau3AI GATC 2 cut(s) 5, 58
Sau96I GGNCC 4 cut(s) 339, 655, 682, 758
ScrFI CCNGG 2 cut(s) 24, 360
SduI GDGCHC 2 cut(s) 215, 628
SfaNI GCATC 2 cut(s) 108, 460
SinI GGWCC 3 cut(s) 655, 682, 758
SmlI CTYRAG 2 cut(s) 425, 457
SmoI CTYRAG 2 cut(s) 425, 457
Sse9I AATT 3 cut(s) 249, 263, 740
SsiI CCGC 3 cut(s) 52, 66, 473
SspMI CTAG 2 cut(s) 56, 302
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 22, 358
TaaI ACNGT 5 cut(s) 16, 34, 111, 542, 677
TaiI ACGT 2 cut(s) 622, 713
TaqI TCGA 1 cut(s) 180
TaqII GACCGA 1 cut(s) 677
TasI AATT 3 cut(s) 249, 263, 740
TauI GCSGC 1 cut(s) 55
TfiI GAWTC 5 cut(s) 82, 203, 582, 609, 799
Tru1I TTAA 4 cut(s) 173, 248, 426, 509
Tru9I TTAA 4 cut(s) 173, 248, 426, 509
TseFI GTSAC 1 cut(s) 529
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 4 cut(s) 273, 276, 567, 822
TspGWI ACGGA 1 cut(s) 79
Tth111I GACNNNGTC 1 cut(s) 712
Vha464I CTTAAG 1 cut(s) 425
VneI GTGCAC 1 cut(s) 624
VpaK11BI GGWCC 3 cut(s) 655, 682, 758
XapI RAATTY 2 cut(s) 263, 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 129
XspI CTAG 2 cut(s) 56, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.