Rroxscaffold_2G00087530

Protein of unknown function (DUF2854)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009598
Physical Location & Seq
Reverse (-)
9551245 .. 9554717
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087530.1

Sequence Viewer

Length: 837 bp
ATGGCAACTGTAACCACCGCAAGAGGAGGAGGAAGCATCTTCTGCTCCCACCATGAACCCTTCACCCGCCACCGCTTTCTCTCTCTCCACCCACCTCGTCCCTTCCTCCACCGCCACCCATCAAAGCCCAACTCCACCTTCCTCACCCATTCCAAGCCCATTGGGCCCATATTGAAACTCACCGCCCCACGAGCCGCCGACACCTCCTCCGTCCCCACCACCGGCGACAAAGCCATAGTCACCGACGACCAGTTCTCCCTCGCCAAGGTTTCATTTGGTTCTATTGGACTAGTTGGTGGGATTACACTGTTAGGGTATGGTTTTGGGGCGTACTTTAATATCCTACCTGGTTCTGAATGGTCAGCATTGATGTTAACATATGGGTTTCCTCTTGCAATCATTGGTATGGCTTTCAAGTATGCAGAACTAAAGCCGGTGCCATGCTTGACTTATTTGGATGCTCAGCAATTAAGGGAAACGGCTGCCACCCCGATTCTTATACAGGTCAGGAATGATGTTACGAGATACCGCTATGGGGATGAGCAGCATTTGGATGAGGCGTTAAAACGAATTTTCCAGTATGGTCAGGGTGGGGGAATTGCTCGGAGGAGTGCGCCCGTTCTACAAAGGATTCGTGAAGAGGTCACAGAAGATGGTAGATACTGTTTAGTCCTGGTGTTTGAGGCAAAATTTCTACAGTTGTCTGATTTTGAACAAAGACAGGCTAAATTTACATCATTCTTCGGACCAGGCATTACAGCTGAAATTGCCAAGGGAGAGGAGGATTTATATGAAGTCAAACTTATTTCCAACTCCAACGTCAACCCCACTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2854 UMP1

Protein Analysis

278

Amino Acids

30.7

Weight (kDa)

8.69

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2854 PF11016 106 - 256 3.3e-47 Protein of unknown function (DUF2854)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 436
AciI CCGC 7 cut(s) 18, 67, 73, 112, 183, 195, 529
AcsI RAATTY 3 cut(s) 570, 689, 728
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 3 cut(s) 221, 265, 535
AgsI TTSAA 3 cut(s) 175, 415, 713
AhlI ACTAGT 1 cut(s) 289
AjnI CCWGG 3 cut(s) 346, 672, 748
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 1 cut(s) 761
AluI AGCT 1 cut(s) 761
AoxI GGCC 1 cut(s) 164
ApaI GGGCCC 1 cut(s) 168
ApeKI GCWGC 2 cut(s) 482, 544
ApoI RAATTY 3 cut(s) 570, 689, 728
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 3 cut(s) 164, 165, 746
AsuHPI GGTGA 4 cut(s) 55, 136, 172, 232
AvaII GGWCC 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 168
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 168
BauI CACGAG 1 cut(s) 189
BbvI GCAGC 2 cut(s) 469, 556
BccI CCATC 2 cut(s) 127, 647
BceAI ACGGC 1 cut(s) 495
BciT130I CCWGG 3 cut(s) 348, 674, 750
BcuI ACTAGT 1 cut(s) 289
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 695
BglI GCCNNNNNGGC 1 cut(s) 163
BisI GCNGC 3 cut(s) 195, 483, 545
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 3 cut(s) 196, 484, 546
Bme1390I CCNGG 3 cut(s) 348, 674, 750
Bme18I GGWCC 1 cut(s) 746
BmgT120I GGNCC 3 cut(s) 164, 165, 746
BmiI GGNNCC 2 cut(s) 166, 438
BmrFI CCNGG 3 cut(s) 348, 674, 750
BmsI GCATC 2 cut(s) 45, 448
Bpu1102I GCTNAGC 1 cut(s) 462
BsaJI CCNNGG 2 cut(s) 264, 771
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 265, 535
Bse118I RCCGGY 2 cut(s) 221, 433
Bse1I ACTGG 2 cut(s) 250, 577
BseBI CCWGG 3 cut(s) 348, 674, 750
BseDI CCNNGG 2 cut(s) 264, 771
BseGI GGATG 3 cut(s) 463, 544, 559
BseLI CCNNNNNNNGG 3 cut(s) 221, 265, 535
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 2 cut(s) 250, 577
BseRI GAGGAG 5 cut(s) 39, 42, 196, 622, 794
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 2 cut(s) 469, 556
BshFI GGCC 1 cut(s) 166
BshNI GGYRCC 1 cut(s) 436
BsiSI CCGG 2 cut(s) 222, 434
BslFI GGGAC 2 cut(s) 84, 197
BslI CCNNNNNNNGG 3 cut(s) 221, 265, 535
BsmFI GGGAC 2 cut(s) 84, 197
BsnI GGCC 1 cut(s) 166
Bsp120I GGGCCC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 168
Bsp1720I GCTNAGC 1 cut(s) 462
BspACI CCGC 7 cut(s) 18, 67, 73, 112, 183, 195, 529
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 1 cut(s) 475
BspLI GGNNCC 2 cut(s) 166, 438
BspT107I GGYRCC 1 cut(s) 436
BsrFI RCCGGY 2 cut(s) 221, 433
BsrI ACTGG 2 cut(s) 250, 577
BssAI RCCGGY 2 cut(s) 221, 433
BssECI CCNNGG 2 cut(s) 264, 771
BssSI CACGAG 1 cut(s) 189
BssT1I CCWWGG 2 cut(s) 264, 771
Bst2BI CACGAG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 348, 674, 750
Bst4CI ACNGT 4 cut(s) 10, 309, 665, 699
Bst6I CTCTTC 1 cut(s) 633
BstAPI GCANNNNNTGC 1 cut(s) 42
BstDEI CTNAG 1 cut(s) 462
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 3 cut(s) 463, 544, 559
BstHHI GCGC 1 cut(s) 616
BstMWI GCNNNNNNNGC 4 cut(s) 42, 163, 191, 767
BstNI CCWGG 3 cut(s) 348, 674, 750
BstSCI CCNGG 3 cut(s) 346, 672, 748
BstSFI CTRYAG 1 cut(s) 695
BstSLI GKGCMC 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 469, 556
BsuRI GGCC 1 cut(s) 166
BtsCI GGATG 3 cut(s) 463, 544, 559
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 305, 828
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 2 cut(s) 221, 433
Cfr13I GGNCC 3 cut(s) 164, 165, 746
CsiI ACCWGGT 1 cut(s) 346
Csp6I GTAC 1 cut(s) 331
CviAII CATG 2 cut(s) 53, 441
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Eco130I CCWWGG 2 cut(s) 264, 771
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 746
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoRII CCWGG 3 cut(s) 346, 672, 748
EcoT14I CCWWGG 2 cut(s) 264, 771
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 2 cut(s) 264, 771
FaeI CATG 2 cut(s) 56, 444
FalI AAGNNNNNCTT 2 cut(s) 786, 818
FaqI GGGAC 2 cut(s) 84, 197
FatI CATG 2 cut(s) 52, 440
FauI CCCGC 1 cut(s) 74
FauNDI CATATG 1 cut(s) 379
Fnu4HI GCNGC 3 cut(s) 195, 483, 545
FokI GGATG 3 cut(s) 470, 551, 566
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 195, 483, 545
FspBI CTAG 1 cut(s) 290
GlaI GCGC 1 cut(s) 615
GluI GCNGC 3 cut(s) 195, 483, 545
HaeIII GGCC 1 cut(s) 166
HapII CCGG 2 cut(s) 222, 434
HhaI GCGC 1 cut(s) 616
Hin1II CATG 2 cut(s) 56, 444
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 2 cut(s) 375, 823
HindII GTYRAC 2 cut(s) 375, 823
HinfI GANTC 2 cut(s) 493, 631
HpaI GTTAAC 1 cut(s) 375
HpaII CCGG 2 cut(s) 222, 434
HphI GGTGA 4 cut(s) 55, 136, 172, 232
Hpy166II GTNNAC 2 cut(s) 375, 823
Hpy188I TCNGA 4 cut(s) 355, 606, 706, 746
Hpy188III TCNNGA 2 cut(s) 508, 635
Hpy8I GTNNAC 2 cut(s) 375, 823
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 70, 112, 148
HpyCH4III ACNGT 4 cut(s) 10, 309, 665, 699
HpyCH4IV ACGT 1 cut(s) 819
HpyCH4V TGCA 3 cut(s) 395, 422, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 163, 191, 767
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 819
Hsp92II CATG 2 cut(s) 56, 444
HspAI GCGC 1 cut(s) 614
KspAI GTTAAC 1 cut(s) 375
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 2 cut(s) 469, 556
LweI GCATC 2 cut(s) 45, 448
MabI ACCWGGT 1 cut(s) 346
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 819
MaeIII GTNAC 4 cut(s) 10, 238, 517, 643
MboII GAAGA 4 cut(s) 31, 650, 662, 733
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 6 cut(s) 467, 570, 597, 689, 728, 765
MmeI TCCRAC 1 cut(s) 834
MseI TTAA 4 cut(s) 336, 374, 470, 563
MslI CAYNNNNRTG 2 cut(s) 404, 552
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 2 cut(s) 222, 434
MspR9I CCNGG 3 cut(s) 348, 674, 750
MvaI CCWGG 3 cut(s) 348, 674, 750
MwoI GCNNNNNNNGC 4 cut(s) 42, 163, 191, 767
NdeI CATATG 1 cut(s) 379
NlaIII CATG 2 cut(s) 56, 444
NlaIV GGNNCC 2 cut(s) 166, 438
NmuCI GTSAC 2 cut(s) 238, 643
PfeI GAWTC 2 cut(s) 493, 631
PkrI GCNGC 3 cut(s) 196, 484, 546
Psp6I CCWGG 3 cut(s) 346, 672, 748
PspGI CCWGG 3 cut(s) 346, 672, 748
PspN4I GGNNCC 2 cut(s) 166, 438
PspOMI GGGCCC 1 cut(s) 164
PspPI GGNCC 3 cut(s) 164, 165, 746
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 2 cut(s) 404, 552
SaqAI TTAA 4 cut(s) 336, 374, 470, 563
SatI GCNGC 3 cut(s) 195, 483, 545
Sau96I GGNCC 3 cut(s) 164, 165, 746
ScrFI CCNGG 3 cut(s) 348, 674, 750
SduI GDGCHC 1 cut(s) 168
SetI ASST 9 cut(s) 97, 140, 206, 270, 349, 507, 645, 763, 822
SexAI ACCWGGT 1 cut(s) 346
SfaNI GCATC 2 cut(s) 45, 448
SfcI CTRYAG 1 cut(s) 695
SgrAI CRCCGGYG 1 cut(s) 221
SinI GGWCC 1 cut(s) 746
SmiMI CAYNNNNRTG 2 cut(s) 404, 552
SpeI ACTAGT 1 cut(s) 289
Sse9I AATT 6 cut(s) 467, 570, 597, 689, 728, 765
SsiI CCGC 7 cut(s) 18, 67, 73, 112, 183, 195, 529
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 3 cut(s) 346, 672, 748
StyI CCWWGG 2 cut(s) 264, 771
TaaI ACNGT 4 cut(s) 10, 309, 665, 699
TaiI ACGT 1 cut(s) 822
TasI AATT 6 cut(s) 467, 570, 597, 689, 728, 765
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 2 cut(s) 493, 631
Tru1I TTAA 4 cut(s) 336, 374, 470, 563
Tru9I TTAA 4 cut(s) 336, 374, 470, 563
TscAI CASTG 2 cut(s) 312, 835
TseFI GTSAC 2 cut(s) 238, 643
TseI GCWGC 2 cut(s) 482, 544
Tsp45I GTSAC 2 cut(s) 238, 643
TspDTI ATGAA 3 cut(s) 69, 261, 807
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 2 cut(s) 312, 835
VpaK11BI GGWCC 1 cut(s) 746
XagI CCTNNNNNAGG 1 cut(s) 263
XapI RAATTY 3 cut(s) 570, 689, 728
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.