MD09G1084900.v1.1

Protein of unknown function (DUF2854)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009598
Physical Location & Seq
Reverse (-)
6081376 .. 6084675
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1084900.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGCAACGCAAGCACTATCTACTCCAACTGCAACTGCAAGAGGACGCAGCAGCTCCTGCCGCCATCATGACGTCAACCGCCGCCGCTTTCTCTCTCTGCGCCACCGTACCACTGCCTCATCCTTATCAAGGCCCATCACCAGCTTCCTCATCCACCTGGATCCCATTGGACGCAGAGCCCACCAACTCTCGAAGTCCAAGCCCAAACTGACCATCCAGAGAGCCGCCGACTCTTCCACTCAGCCTTCCACCGTCACCTCCTCCTCTGCCGCCGGCAAAGCCATCGTTCCCGACGACCAGTTCTCCCTTGCTAAGGTTTCGTTTGGGGTTATTGGTCTGGGTGTTGGGGTTTCACTCTTGTCGTATGGTTTCGGAGCGTATTTTAATATCCTTCCAGGGTCCGAATGGTCGGCATTGATGCTCACATACGGCTTCCCTCTTGCAATCATTGGTATGGCTTTCAAGTATGCAGAACTAAAGCCAGTGCCATGCTTGACTTATTTGGATGCGCTACAATTAAGGGAAACAAGTGCCACCCCAATCCTTACACAGGTTAGGAATGATGTTACAAGGTATCGTTATGGAGATGAGCAGCATTTGGATGAGGCGTTGAAACGAATCTTCCAGTATGGTCAGGGTGGAGGAATTGCTCGGAGGAGTGCACCTACTCTCCAAAGTATTCGTGAAGAGGTCACCGGAGATGGTAGATACTCTCTAGTCCTGGTGTTTGAGGCAAAAGCTCTGCAGTTGTCAGATTTTGAAGAAAGACAGGCTAAGTTTACTTCATTCTTCGGACCAGGAATCACAGCTGAAGTTGGGAAGGGAGAGAATGATCTATACGAAGTCCGACTTATTTCCAACACCAACGCTAACCCTACTGCATCACCGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2854

Protein Analysis

298

Amino Acids

32.43

Weight (kDa)

9.63

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2854 PF11016 122 - 272 3.4e-47 Protein of unknown function (DUF2854)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AciI CCGC 6 cut(s) 61, 79, 82, 85, 225, 270
AclWI GGATC 2 cut(s) 155, 168
AcuI CTGAAG 1 cut(s) 831
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 129, 313, 550
AgsI TTSAA 3 cut(s) 463, 613, 761
AjnI CCWGG 4 cut(s) 156, 394, 720, 796
AluBI AGCT 4 cut(s) 54, 144, 740, 809
AluI AGCT 4 cut(s) 54, 144, 740, 809
Alw21I GWGCWC 1 cut(s) 664
Alw44I GTGCAC 1 cut(s) 660
AlwI GGATC 2 cut(s) 155, 168
AlwNI CAGNNNCTG 1 cut(s) 57
AoxI GGCC 1 cut(s) 131
ApaLI GTGCAC 1 cut(s) 660
ApeKI GCWGC 3 cut(s) 48, 51, 592
AspLEI GCGC 2 cut(s) 102, 511
AspS9I GGNCC 3 cut(s) 132, 399, 794
AsuHPI GGTGA 4 cut(s) 130, 247, 685, 876
AvaII GGWCC 2 cut(s) 399, 794
BaeGI GKGCMC 1 cut(s) 664
BamHI GGATCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 3 cut(s) 60, 63, 604
BccI CCATC 5 cut(s) 72, 143, 221, 290, 695
BceAI ACGGC 1 cut(s) 445
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciT130I CCWGG 4 cut(s) 158, 396, 722, 798
BfaI CTAG 1 cut(s) 716
BfmI CTRYAG 1 cut(s) 743
BisI GCNGC 8 cut(s) 49, 52, 61, 82, 85, 225, 270, 593
BlsI GCNGC 8 cut(s) 50, 53, 62, 83, 86, 226, 271, 594
Bme1390I CCNGG 4 cut(s) 158, 396, 722, 798
Bme18I GGWCC 2 cut(s) 399, 794
BmgT120I GGNCC 3 cut(s) 132, 399, 794
BmiI GGNNCC 2 cut(s) 162, 400
BmrFI CCNGG 4 cut(s) 158, 396, 722, 798
BmsI GCATC 3 cut(s) 408, 496, 890
Bpu10I CCTNAGC 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 395
BsaWI WCCGGW 1 cut(s) 695
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 654, 684
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 313, 550
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 3 cut(s) 298, 482, 625
BseBI CCWGG 4 cut(s) 158, 396, 722, 798
BseDI CCNNGG 1 cut(s) 395
BseGI GGATG 5 cut(s) 119, 150, 213, 511, 607
BseLI CCNNNNNNNGG 3 cut(s) 129, 313, 550
BseMII CTCAG 1 cut(s) 254
BseNI ACTGG 3 cut(s) 298, 482, 625
BseRI GAGGAG 3 cut(s) 250, 253, 670
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 3 cut(s) 60, 63, 604
BshFI GGCC 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 2 cut(s) 273, 696
BslI CCNNNNNNNGG 3 cut(s) 129, 313, 550
BsnI GGCC 1 cut(s) 133
Bsp1286I GDGCHC 2 cut(s) 181, 664
Bsp143I GATC 2 cut(s) 160, 832
BspACI CCGC 6 cut(s) 61, 79, 82, 85, 225, 270
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 1 cut(s) 253
BspHI TCATGA 1 cut(s) 67
BspLI GGNNCC 2 cut(s) 162, 400
BspMAI CTGCAG 1 cut(s) 747
BspPI GGATC 2 cut(s) 155, 168
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 3 cut(s) 298, 482, 625
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 2 cut(s) 160, 832
BssNI GRCGYC 1 cut(s) 72
Bst2UI CCWGG 4 cut(s) 158, 396, 722, 798
Bst4CI ACNGT 3 cut(s) 107, 253, 888
Bst6I CTCTTC 2 cut(s) 238, 681
BstACI GRCGYC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 12, 274
BstDEI CTNAG 3 cut(s) 240, 312, 774
BstEII GGTNACC 1 cut(s) 691
BstENI CCTNNNNNAGG 3 cut(s) 127, 311, 548
BstF5I GGATG 5 cut(s) 119, 150, 213, 511, 607
BstHHI GCGC 2 cut(s) 102, 511
BstKTI GATC 2 cut(s) 163, 835
BstMBI GATC 2 cut(s) 160, 832
BstMWI GCNNNNNNNGC 4 cut(s) 11, 57, 60, 278
BstNI CCWGG 4 cut(s) 158, 396, 722, 798
BstPI GGTNACC 1 cut(s) 691
BstSCI CCNGG 4 cut(s) 156, 394, 720, 796
BstSFI CTRYAG 1 cut(s) 743
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 3 cut(s) 60, 63, 604
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuRI GGCC 1 cut(s) 133
BtsCI GGATG 5 cut(s) 119, 150, 213, 511, 607
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 489
Cac8I GCNNGC 2 cut(s) 12, 274
CaiI CAGNNNCTG 1 cut(s) 57
CciI TCATGA 1 cut(s) 67
CfoI GCGC 2 cut(s) 102, 511
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 3 cut(s) 132, 399, 794
CseI GACGC 2 cut(s) 54, 180
Csp6I GTAC 1 cut(s) 108
CviAII CATG 2 cut(s) 68, 489
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 3 cut(s) 240, 312, 774
DpnI GATC 2 cut(s) 162, 834
DpnII GATC 2 cut(s) 160, 832
Eam1104I CTCTTC 2 cut(s) 238, 681
EarI CTCTTC 2 cut(s) 238, 681
Eco24I GRGCYC 1 cut(s) 181
Eco47I GGWCC 2 cut(s) 399, 794
Eco57I CTGAAG 1 cut(s) 831
Eco91I GGTNACC 1 cut(s) 691
EcoNI CCTNNNNNAGG 3 cut(s) 127, 311, 548
EcoO65I GGTNACC 1 cut(s) 691
EcoRII CCWGG 4 cut(s) 156, 394, 720, 796
EcoT38I GRGCYC 1 cut(s) 181
FaeI CATG 2 cut(s) 71, 492
FaiI YATR 9 cut(s) 69, 366, 427, 455, 468, 490, 582, 630, 838
FalI AAGNNNNNCTT 2 cut(s) 834, 866
FatI CATG 2 cut(s) 67, 488
Fnu4HI GCNGC 8 cut(s) 49, 52, 61, 82, 85, 225, 270, 593
FokI GGATG 5 cut(s) 106, 137, 200, 518, 614
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 8 cut(s) 49, 52, 61, 82, 85, 225, 270, 593
FspBI CTAG 1 cut(s) 716
GlaI GCGC 2 cut(s) 101, 510
GluI GCNGC 8 cut(s) 49, 52, 61, 82, 85, 225, 270, 593
HaeIII GGCC 1 cut(s) 133
HapII CCGG 2 cut(s) 273, 696
HgaI GACGC 2 cut(s) 54, 180
HhaI GCGC 2 cut(s) 102, 511
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 2 cut(s) 71, 492
Hin6I GCGC 2 cut(s) 100, 509
HinP1I GCGC 2 cut(s) 100, 509
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 3 cut(s) 230, 618, 801
HpaII CCGG 2 cut(s) 273, 696
HphI GGTGA 4 cut(s) 130, 247, 685, 876
Hpy166II GTNNAC 3 cut(s) 76, 662, 780
Hpy188I TCNGA 6 cut(s) 374, 403, 654, 754, 794, 848
Hpy188III TCNNGA 5 cut(s) 68, 190, 217, 290, 683
Hpy8I GTNNAC 3 cut(s) 76, 662, 780
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 3 cut(s) 255, 401, 814
HpyCH4III ACNGT 3 cut(s) 107, 253, 888
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 7 cut(s) 32, 38, 443, 470, 662, 745, 881
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 57, 60, 278
HpyF3I CTNAG 3 cut(s) 240, 312, 774
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 2 cut(s) 71, 492
HspAI GCGC 2 cut(s) 100, 509
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 160, 832
LmnI GCTCC 2 cut(s) 59, 374
Lsp1109I GCAGC 3 cut(s) 60, 63, 604
LweI GCATC 3 cut(s) 408, 496, 890
MaeI CTAG 1 cut(s) 716
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 3 cut(s) 253, 565, 691
MalI GATC 2 cut(s) 162, 834
MboI GATC 2 cut(s) 160, 832
MboII GAAGA 5 cut(s) 225, 613, 698, 773, 781
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 2 cut(s) 181, 664
MluCI AATT 2 cut(s) 515, 645
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 3 cut(s) 50, 871, 882
MroNI GCCGGC 1 cut(s) 272
MseI TTAA 2 cut(s) 384, 518
MslI CAYNNNNRTG 2 cut(s) 452, 600
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 2 cut(s) 273, 696
MspR9I CCNGG 4 cut(s) 158, 396, 722, 798
MvaI CCWGG 4 cut(s) 158, 396, 722, 798
MwoI GCNNNNNNNGC 4 cut(s) 11, 57, 60, 278
NaeI GCCGGC 1 cut(s) 274
NdeII GATC 2 cut(s) 160, 832
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 2 cut(s) 71, 492
NlaIV GGNNCC 2 cut(s) 162, 400
NmuCI GTSAC 2 cut(s) 253, 691
PagI TCATGA 1 cut(s) 67
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 618, 801
PkrI GCNGC 8 cut(s) 50, 53, 62, 83, 86, 226, 271, 594
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Psp6I CCWGG 4 cut(s) 156, 394, 720, 796
PspEI GGTNACC 1 cut(s) 691
PspGI CCWGG 4 cut(s) 156, 394, 720, 796
PspN4I GGNNCC 2 cut(s) 162, 400
PspPI GGNCC 3 cut(s) 132, 399, 794
PstI CTGCAG 1 cut(s) 747
PstNI CAGNNNCTG 1 cut(s) 57
PsuI RGATCY 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
RseI CAYNNNNRTG 2 cut(s) 452, 600
SaqAI TTAA 2 cut(s) 384, 518
SatI GCNGC 8 cut(s) 49, 52, 61, 82, 85, 225, 270, 593
Sau3AI GATC 2 cut(s) 160, 832
Sau96I GGNCC 3 cut(s) 132, 399, 794
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 4 cut(s) 158, 396, 722, 798
SduI GDGCHC 2 cut(s) 181, 664
SfaNI GCATC 3 cut(s) 408, 496, 890
SfcI CTRYAG 1 cut(s) 743
SinI GGWCC 2 cut(s) 399, 794
SmiMI CAYNNNNRTG 2 cut(s) 452, 600
Sse9I AATT 2 cut(s) 515, 645
SsiI CCGC 6 cut(s) 61, 79, 82, 85, 225, 270
SspMI CTAG 1 cut(s) 716
StyD4I CCNGG 4 cut(s) 156, 394, 720, 796
TaaI ACNGT 3 cut(s) 107, 253, 888
TaiI ACGT 1 cut(s) 75
TaqI TCGA 1 cut(s) 191
TasI AATT 2 cut(s) 515, 645
TauI GCSGC 5 cut(s) 63, 84, 87, 227, 272
TfiI GAWTC 2 cut(s) 618, 801
Tru1I TTAA 2 cut(s) 384, 518
Tru9I TTAA 2 cut(s) 384, 518
TscAI CASTG 2 cut(s) 118, 489
TseFI GTSAC 2 cut(s) 253, 691
TseI GCWGC 3 cut(s) 48, 51, 592
Tsp45I GTSAC 2 cut(s) 253, 691
TspDTI ATGAA 1 cut(s) 774
TspRI CASTG 2 cut(s) 118, 489
VneI GTGCAC 1 cut(s) 660
VpaK11BI GGWCC 2 cut(s) 399, 794
XagI CCTNNNNNAGG 3 cut(s) 127, 311, 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 402
XspI CTAG 1 cut(s) 716
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.