AT5G39440

Non-specific serine threonine protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005352
Physical Location & Seq
Forward (+)
15781907 .. 15784699
2793 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39440.1

Sequence Viewer

Length: 1485 bp
ATGGATGGATCATCGGAAAAAACTACCAATAAATTAGTATCGATTCTACCAAATTATAGGATTGGTAAGACTCTTGGACATGGATCCTTTGCGAAAGTGAAATTAGCACTGCATGTAGCTACTGGTCACAAAGTTGCTATCAAAATTCTTAATCGCTCTAAGATTAAGAACATGGGTATTGAGATCAAAGTACAAAGGGAGATCAAAATTCTCAGATTTTTGATGCATCCTCATATCATCCGTCAATATGAAGTTATAGAGACGCCAAATGACATTTATGTTGTGATGGAATATGTTAAGTCTGGAGAACTATTTGACTATATTGTAGAGAAAGGTAAATTACAGGAAGACGAGGCTCGTCATCTTTTTCAGCAGATCATATCTGGTGTAGAGTATTGTCATCGCAATATGATCGTTCATAGAGATCTTAAGCCCGAGAATGTGCTTTTGGATTCGCAATGCAACATAAAGATTGTTGACTTTGGATTGAGTAATGTCATGCATGATGGTCATTTTTTAAAGACTAGTTGTGGAAGCCCTAATTATGCTGCTCCAGAGGTTATTTCAGGAAAACCATATGGACCAGATGTAGATATATGGAGTTGTGGGGTGATATTATATGCTCTGTTGTGTGGTACTCTTCCTTTCGATGACGAAAATATTCCTAATGTATTCGAGAAAATAAAGAGAGGAATGTATACTCTTCCAAATCATTTATCACATTTTGCTAGAGATTTGATACCGAGAATGCTTATGGTTGATCCTACTATGAGAATTAGCATTACTGAGATTCGTCAACATCCTTGGTTCAATAATCATCTTCCTCTTTATCTTTCTATTCCTCCATTAGATACCATAGACCAAGCTAAAAAGATTGAGGAGGAGATAATTCAAAACGTGGTTAACATAGGGTTTGATAGAAACCATGTTGTTGATTCTCTTGCCAATAGAATCCAAAACGAGGCTACAGTTGCATACCATCTAATCCTAGACAACCGAAACCAAAATTCTGTTCCCAATGACCCTTTCCAATCCAAGTTTAAAGAGATTTCGGATGGTATATTTAATTCGACGTTACCAGTTCAAAATATTACTTCACATGTTGGCCATTCATTTTCGGCATTGTATGGACTAAAATCCAATGTCAAAGATGACAAAACATGGACCCTTGGACTTCAGTCTCAAGGGAGTCCTTATGATATCATGACTGAAATTTTTAAAGCTCTTCAAAATCTAAAAATATGCTGGAAAAAAATCGGACTCTACAATATAAAATGTAGATGGGTCCGAAGCTTTGCTTACTATAAGAATCATACAATTGAGGATGAATGTGCCATCATCTTACCGACCGTCATCAAATTTGAAATTCAGCTATACAAAGTACGCGAAGGCAAATATTTGCTGGACATCCTAAGAATTGATGGACCTCAGTTTATCTTCTTTGATCTATGTGTTGCGTTTCTCAGAGAGCTAGGTGTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0007584 GO:0008150 GO:0008152 GO:0009593 GO:0009594 GO:0009605 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010050 GO:0010150 GO:0010182 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051606 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080022 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
KA1 Pkinase UBA

Protein Analysis

494

Amino Acids

56.7

Weight (kDa)

7.98

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 19 - 270 1.8e-82 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 21 - 266 1.8e-41 Protein tyrosine and serine/threonine kinase
RIO1 PF01163 60 - 162 5.2e-06 RIO domain
Kinase-like PF14531 111 - 250 3.5e-06 Kinase-like
UBA PF00627 292 - 328 7.1e-07 UBA/TS-N domain
AdenylateSensor PF16579 388 - 475 1.3e-10 Adenylate sensor of SNF1-like protein kinase
KA1 PF02149 450 - 491 1.1e-12 Kinase associated domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 698
AccII CGCG 1 cut(s) 1386
AclWI GGATC 4 cut(s) 16, 78, 91, 755
AcoI YGGCCR 1 cut(s) 1105
AcsI RAATTY 6 cut(s) 144, 207, 1006, 1212, 1358, 1365
AcuI CTGAAG 1 cut(s) 1160
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 3 cut(s) 192, 637, 1383
AfiI CCNNNNNNNGG 1 cut(s) 961
AflII CTTAAG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 1099
AgsI TTSAA 5 cut(s) 811, 893, 1085, 1229, 1364
AhlI ACTAGT 1 cut(s) 524
AjuI GAANNNNNNNTTGG 6 cut(s) 431, 463, 996, 1010, 1028, 1042
AluBI AGCT 6 cut(s) 119, 866, 1223, 1293, 1372, 1471
AluI AGCT 6 cut(s) 119, 866, 1223, 1293, 1372, 1471
Alw26I GTCTC 2 cut(s) 254, 1185
AlwI GGATC 4 cut(s) 16, 78, 91, 755
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 1 cut(s) 1105
ApeKI GCWGC 1 cut(s) 548
ApoI RAATTY 6 cut(s) 144, 207, 1006, 1212, 1358, 1365
Asp700I GAANNNNTTC 1 cut(s) 660
AspS9I GGNCC 4 cut(s) 581, 1164, 1285, 1424
AsuHPI GGTGA 1 cut(s) 622
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 4 cut(s) 581, 1164, 1285, 1424
BalI TGGCCA 1 cut(s) 1107
BamHI GGATCC 1 cut(s) 83
BbsI GAAGAC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 535
BccI CCATC 7 cut(s) 280, 500, 987, 1049, 1275, 1343, 1415
BcgI CGANNNNNNTGC 2 cut(s) 394, 428
BcoDI GTCTC 2 cut(s) 254, 1185
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 4 cut(s) 525, 729, 989, 1472
BfmI CTRYAG 1 cut(s) 966
BfrI CTTAAG 1 cut(s) 428
BglII AGATCT 1 cut(s) 424
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
Bme18I GGWCC 4 cut(s) 581, 1164, 1285, 1424
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 4 cut(s) 581, 1164, 1285, 1424
BmiI GGNNCC 3 cut(s) 85, 1166, 1286
BmsI GCATC 2 cut(s) 213, 235
BoxI GACNNNNGTC 1 cut(s) 1177
BpiI GAAGAC 1 cut(s) 354
BpmI CTGGAG 2 cut(s) 324, 537
BpuEI CTTGAG 1 cut(s) 1167
Bsa29I ATCGAT 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 803, 1168
Bsc4I CCNNNNNNNGG 1 cut(s) 961
Bse1I ACTGG 2 cut(s) 127, 1079
Bse3DI GCAATG 1 cut(s) 464
BseCI ATCGAT 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 803, 1168
BseGI GGATG 7 cut(s) 10, 226, 237, 799, 1060, 1330, 1407
BseLI CCNNNNNNNGG 1 cut(s) 961
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 4 cut(s) 226, 777, 1442, 1477
BseNI ACTGG 2 cut(s) 127, 1079
BseRI GAGGAG 2 cut(s) 893, 896
BseXI GCAGC 1 cut(s) 535
Bsh1236I CGCG 1 cut(s) 1386
Bsh1285I CGRYCG 1 cut(s) 1350
BshFI GGCC 1 cut(s) 1107
BshVI ATCGAT 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 1350
BsiHKCI CYCGRG 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 961
BsmAI GTCTC 2 cut(s) 254, 1185
BsmBI CGTCTC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 1 cut(s) 1107
BsoBI CYCGRG 1 cut(s) 434
Bsp143I GATC 9 cut(s) 8, 83, 183, 201, 375, 411, 424, 760, 1444
BspANI GGCC 1 cut(s) 1107
BspCNI CTCAG 4 cut(s) 225, 778, 1441, 1476
BspDI ATCGAT 1 cut(s) 41
BspFNI CGCG 1 cut(s) 1386
BspHI TCATGA 1 cut(s) 1203
BspLI GGNNCC 3 cut(s) 85, 1166, 1286
BspPI GGATC 4 cut(s) 16, 78, 91, 755
BspQI GCTCTTC 1 cut(s) 1230
BspTI CTTAAG 1 cut(s) 428
BsrDI GCAATG 1 cut(s) 464
BsrI ACTGG 2 cut(s) 127, 1079
BssECI CCNNGG 2 cut(s) 803, 1168
BssMI GATC 9 cut(s) 8, 83, 183, 201, 375, 411, 424, 760, 1444
BssNAI GTATAC 1 cut(s) 699
BssNI GRCGYC 1 cut(s) 263
BssT1I CCWWGG 2 cut(s) 803, 1168
Bst1107I GTATAC 1 cut(s) 699
Bst4CI ACNGT 2 cut(s) 970, 1351
Bst6I CTCTTC 3 cut(s) 645, 708, 1230
BstACI GRCGYC 1 cut(s) 263
BstAFI CTTAAG 1 cut(s) 428
BstDEI CTNAG 6 cut(s) 159, 212, 786, 1412, 1428, 1463
BstF5I GGATG 7 cut(s) 10, 226, 237, 799, 1060, 1330, 1407
BstFNI CGCG 1 cut(s) 1386
BstKTI GATC 9 cut(s) 11, 86, 186, 204, 378, 414, 427, 763, 1447
BstMAI GTCTC 2 cut(s) 254, 1185
BstMBI GATC 9 cut(s) 8, 83, 183, 201, 375, 411, 424, 760, 1444
BstMCI CGRYCG 1 cut(s) 1350
BstMWI GCNNNNNNNGC 1 cut(s) 971
BstNSI RCATGY 2 cut(s) 116, 1103
BstPAI GACNNNNGTC 1 cut(s) 1177
BstSFI CTRYAG 1 cut(s) 966
BstUI CGCG 1 cut(s) 1386
BstV1I GCAGC 1 cut(s) 535
BstV2I GAAGAC 1 cut(s) 354
BstX2I RGATCY 2 cut(s) 83, 424
BstYI RGATCY 2 cut(s) 83, 424
BstZ17I GTATAC 1 cut(s) 699
Bsu15I ATCGAT 1 cut(s) 41
BsuRI GGCC 1 cut(s) 1107
BsuTUI ATCGAT 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 386
BtsCI GGATG 7 cut(s) 10, 226, 237, 799, 1060, 1330, 1407
BtsI GCAGTG 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 107
CciI TCATGA 1 cut(s) 1203
Cfr13I GGNCC 4 cut(s) 581, 1164, 1285, 1424
ClaI ATCGAT 1 cut(s) 41
CseI GACGC 1 cut(s) 271
Csp6I GTAC 3 cut(s) 191, 636, 1382
CviAII CATG 9 cut(s) 80, 113, 172, 499, 503, 926, 1100, 1161, 1204
CviQI GTAC 3 cut(s) 191, 636, 1382
DdeI CTNAG 6 cut(s) 159, 212, 786, 1412, 1428, 1463
DpnI GATC 9 cut(s) 10, 85, 185, 203, 377, 413, 426, 762, 1446
DpnII GATC 9 cut(s) 8, 83, 183, 201, 375, 411, 424, 760, 1444
DraI TTTAAA 3 cut(s) 519, 1042, 1219
EaeI YGGCCR 1 cut(s) 1105
Eam1104I CTCTTC 3 cut(s) 645, 708, 1230
EarI CTCTTC 3 cut(s) 645, 708, 1230
Eco130I CCWWGG 2 cut(s) 803, 1168
Eco32I GATATC 1 cut(s) 1201
Eco47I GGWCC 4 cut(s) 581, 1164, 1285, 1424
Eco57I CTGAAG 1 cut(s) 1160
Eco88I CYCGRG 1 cut(s) 434
EcoRV GATATC 1 cut(s) 1201
EcoT14I CCWWGG 2 cut(s) 803, 1168
EcoT22I ATGCAT 2 cut(s) 228, 504
ErhI CCWWGG 2 cut(s) 803, 1168
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 9 cut(s) 83, 116, 175, 502, 506, 929, 1103, 1164, 1207
FalI AAGNNNNNCTT 2 cut(s) 1282, 1314
FatI CATG 9 cut(s) 79, 112, 171, 498, 502, 925, 1099, 1160, 1203
FauNDI CATATG 1 cut(s) 577
FblI GTMKAC 1 cut(s) 698
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 7 cut(s) 17, 213, 224, 786, 1067, 1337, 1394
Fsp4HI GCNGC 1 cut(s) 549
FspBI CTAG 4 cut(s) 525, 729, 989, 1472
GluI GCNGC 1 cut(s) 549
GsuI CTGGAG 2 cut(s) 324, 537
HaeIII GGCC 1 cut(s) 1107
HgaI GACGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 9 cut(s) 83, 116, 175, 502, 506, 929, 1103, 1164, 1207
HincII GTYRAC 3 cut(s) 478, 797, 904
HindII GTYRAC 3 cut(s) 478, 797, 904
HindIII AAGCTT 1 cut(s) 1291
HinfI GANTC 9 cut(s) 43, 70, 452, 790, 935, 951, 1189, 1260, 1309
HpaI GTTAAC 1 cut(s) 904
HphI GGTGA 1 cut(s) 622
Hpy166II GTNNAC 4 cut(s) 478, 699, 797, 904
Hpy188I TCNGA 6 cut(s) 16, 215, 1054, 1259, 1289, 1466
Hpy188III TCNNGA 5 cut(s) 303, 554, 567, 676, 1204
Hpy8I GTNNAC 4 cut(s) 478, 699, 797, 904
Hpy99I CGWCG 1 cut(s) 1075
HpyAV CCTTC 1 cut(s) 1382
HpyCH4III ACNGT 2 cut(s) 970, 1351
HpyCH4IV ACGT 2 cut(s) 897, 1073
HpyCH4V TGCA 5 cut(s) 112, 226, 462, 502, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 971
HpyF3I CTNAG 6 cut(s) 159, 212, 786, 1412, 1428, 1463
HpySE526I ACGT 2 cut(s) 897, 1073
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 9 cut(s) 83, 116, 175, 502, 506, 929, 1103, 1164, 1207
KspAI GTTAAC 1 cut(s) 904
Kzo9I GATC 9 cut(s) 8, 83, 183, 201, 375, 411, 424, 760, 1444
LguI GCTCTTC 1 cut(s) 1230
LmnI GCTCC 1 cut(s) 556
Lsp1109I GCAGC 1 cut(s) 535
LweI GCATC 2 cut(s) 213, 235
MaeI CTAG 4 cut(s) 525, 729, 989, 1472
MaeII ACGT 2 cut(s) 897, 1073
MaeIII GTNAC 2 cut(s) 125, 1074
MalI GATC 9 cut(s) 10, 85, 185, 203, 377, 413, 426, 762, 1446
MboI GATC 9 cut(s) 8, 83, 183, 201, 375, 411, 424, 760, 1444
MboII GAAGA 6 cut(s) 359, 632, 695, 812, 1217, 1429
MfeI CAATTG 1 cut(s) 1317
MflI RGATCY 2 cut(s) 83, 424
MlsI TGGCCA 1 cut(s) 1107
MluNI TGGCCA 1 cut(s) 1107
MlyI GAGTC 3 cut(s) 64, 1198, 1254
Mox20I TGGCCA 1 cut(s) 1107
Mph1103I ATGCAT 2 cut(s) 228, 504
MroXI GAANNNNTTC 1 cut(s) 660
MscI TGGCCA 1 cut(s) 1107
MseI TTAA 9 cut(s) 150, 165, 297, 429, 518, 903, 1041, 1065, 1218
Msp20I TGGCCA 1 cut(s) 1107
MspCI CTTAAG 1 cut(s) 428
MunI CAATTG 1 cut(s) 1317
Mva1269I GAATGC 1 cut(s) 753
MvnI CGCG 1 cut(s) 1386
MwoI GCNNNNNNNGC 1 cut(s) 971
NdeI CATATG 1 cut(s) 577
NdeII GATC 9 cut(s) 8, 83, 183, 201, 375, 411, 424, 760, 1444
NlaIII CATG 9 cut(s) 83, 116, 175, 502, 506, 929, 1103, 1164, 1207
NlaIV GGNNCC 3 cut(s) 85, 1166, 1286
NmuCI GTSAC 1 cut(s) 125
NsiI ATGCAT 2 cut(s) 228, 504
NspI RCATGY 2 cut(s) 116, 1103
PagI TCATGA 1 cut(s) 1203
PciI ACATGT 1 cut(s) 1099
PciSI GCTCTTC 1 cut(s) 1230
PctI GAATGC 1 cut(s) 753
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 6 cut(s) 43, 452, 790, 935, 951, 1309
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 3 cut(s) 64, 1197, 1254
PpsI GAGTC 3 cut(s) 64, 1197, 1254
PscI ACATGT 1 cut(s) 1099
PshAI GACNNNNGTC 1 cut(s) 1177
PspN4I GGNNCC 3 cut(s) 85, 1166, 1286
PspPI GGNCC 4 cut(s) 581, 1164, 1285, 1424
PsuI RGATCY 2 cut(s) 83, 424
RsaI GTAC 3 cut(s) 192, 637, 1383
RsaNI GTAC 3 cut(s) 191, 636, 1382
SapI GCTCTTC 1 cut(s) 1230
SaqAI TTAA 9 cut(s) 150, 165, 297, 429, 518, 903, 1041, 1065, 1218
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 9 cut(s) 8, 83, 183, 201, 375, 411, 424, 760, 1444
Sau96I GGNCC 4 cut(s) 581, 1164, 1285, 1424
SchI GAGTC 3 cut(s) 64, 1198, 1254
SfaNI GCATC 2 cut(s) 213, 235
SfcI CTRYAG 1 cut(s) 966
SinI GGWCC 4 cut(s) 581, 1164, 1285, 1424
SmlI CTYRAG 2 cut(s) 428, 1182
SmoI CTYRAG 2 cut(s) 428, 1182
SpeI ACTAGT 1 cut(s) 524
SspI AATATT 3 cut(s) 661, 1090, 1397
SspMI CTAG 4 cut(s) 525, 729, 989, 1472
StyI CCWWGG 2 cut(s) 803, 1168
TaaI ACNGT 2 cut(s) 970, 1351
TaiI ACGT 2 cut(s) 900, 1076
TaqI TCGA 4 cut(s) 41, 648, 675, 1070
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 6 cut(s) 43, 452, 790, 935, 951, 1309
Tru1I TTAA 9 cut(s) 150, 165, 297, 429, 518, 903, 1041, 1065, 1218
Tru9I TTAA 9 cut(s) 150, 165, 297, 429, 518, 903, 1041, 1065, 1218
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 4 cut(s) 264, 407, 1101, 1341
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 1 cut(s) 114
Vha464I CTTAAG 1 cut(s) 428
VpaK11BI GGWCC 4 cut(s) 581, 1164, 1285, 1424
XapI RAATTY 6 cut(s) 144, 207, 1006, 1212, 1358, 1365
XceI RCATGY 2 cut(s) 116, 1103
XmiI GTMKAC 1 cut(s) 698
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 4 cut(s) 525, 729, 989, 1472
Zsp2I ATGCAT 2 cut(s) 228, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.