Rroxscaffold_2G00083770

SNF1-related protein kinase catalytic subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005352
Physical Location & Seq
Reverse (-)
6388919 .. 6394330
5412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083770.1

Sequence Viewer

Length: 1614 bp
ATGACTTGTGTAGAAGTCATGAAGTCTGTAGCGCGTTGCTCGTTCCAAGTCTGGAAAATGGATGGACCGGTTGGCCGTGGAGGCAGCAGCGCAGATGCGTATTTACCGAATTACAAGCTTGGAAAAACTCTTGGTATTGGTTCTTTTGGCAAGGTCAAAATTGCAGAGCATGCATTAACTGGCCACAAAGTTGCTATCAAGATCCTTAACCGGCGCAAGATAAAGAACATGGAAATGGAAGAGAAAGTGAGAAGAGAAATCAAAATATTAAGACTGTTTATGCACCCTCACATTATACGACTCTATGAGGTCATTGAAACACCATCGGACATTTATGTTGTGATGGAGTATGTGAAGTCTGGGGAGCTTTTTGATTATATAGTAGAGAAGGGTAGGCTACAGGAGGATGAAGCTCGTAACTTTTTTCAGCAGATAATATCTGGCGTGGAGTACTGTCACAGGAATATGGTCGTTCATAGAGACCTCAAGCCAGAAAATTTGCTTTTGGATTCCAAATGCAATGTGAAGATTGCTGATTTTGGCCTAAGCAATATTATGCGTGATGGCCATTTTCTTAAGACAAGTTGTGGCAGCCCAAATTATGCTGCTCCAGAGGTTATTTCTGGCAAACTATATGCTGGGCCCGAAGTAGATGTATGGAGTTGTGGAGTGATATTATATGCTCTTCTTTGTGGTACTCTTCCTTTTGACGATGAAAACATTCCCAACTTGTTCAAGAAAATTAAGGGCGGGATATACACTCTTCCAAGCCATTTGTCACCTGGTGCAAGGGACTTAATCCCAAGAATGCTCGTGGTGGATCCAATGAAGCGAATGACCATCCCTGAGATTCGTCAGCATCCATGGTTCCAGGCTCATCTTCCTCGTTACTTAGCTGTGCCCCCACCAGATACAATGCAGCAAGCAAAGAAGATTGATGAAGAAATCTTACAGGAAGTAGTGAAGATGGGATTTGACAGGAACCATCTGGTTGAATCTCTGCGTGGTAGATTGCAAAATGAGGGAACCGTTGCATACTATTTATTATTGGACAACCGGTTTCGTGTATCCAGTGGCTATCTTGGGGCCGAGTTTCAGGAGACTGTGGAATCTGGTTTCAACCGTATGCATCAAAGTGAGCCTGCTTCTTCACCTGTTGGGCACCGCCTTCCAGGATATATGGAGTATCAAGGAATGGGCTCTTCACCTTTTAGACAACAGTTTCCTGTTGAAAGGAAATGGGCTCTTGGACTTCAGTCTCGGGCTCATCCTCGTGAAATAATGACCGAAGTCCTTAAAGCTTTACAAGAATTGCGGGTGTGTTGGAAGAAGATAGGACACTACAACATGAAGTGTAGGTGGGTTCCTGGCACTCCTGGTCAACATGAAGGCATGGTTGACAATCCTGTAAACAATAACCATTACTTTGGGGACGAATCCAGCATCATAGAAAATGATGGCGTTATGAAGACACCCAATGTTGTCAAGTTTGAAGTGCAGCTTTTCAAAACTCGGGAGGAGAAGTATCTGCTTGATCTGCAGAGGGTCCAGGGTCCGCAGTTTCTCTTCCTGGATCTCTGTGCCGCTTTCCTTGCACAGCTTCGTGTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0007584 GO:0008150 GO:0008152 GO:0009593 GO:0009594 GO:0009605 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010050 GO:0010150 GO:0010182 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051606 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080022 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
KA1 Pkinase UBA

Protein Analysis

537

Amino Acids

61.4

Weight (kDa)

8.71

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 38 - 290 5.4e-83 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 39 - 286 4.5e-41 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 138 - 238 6.4e-07 Kinase-like
UBA PF00627 312 - 348 2.1e-07 UBA/TS-N domain
KA1 PF02149 494 - 536 3.4e-13 Kinase associated domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1161
AccII CGCG 1 cut(s) 34
AciI CCGC 5 cut(s) 750, 1165, 1315, 1556, 1584
AclWI GGATC 4 cut(s) 196, 815, 828, 1581
AcoI YGGCCR 3 cut(s) 73, 181, 565
AcsI RAATTY 1 cut(s) 496
AcuI CTGAAG 1 cut(s) 1238
AdeI CACNNNGTG 1 cut(s) 785
AfaI GTAC 2 cut(s) 452, 697
AflII CTTAAG 1 cut(s) 575
AgeI ACCGGT 2 cut(s) 67, 1056
AgsI TTSAA 7 cut(s) 317, 736, 995, 1120, 1232, 1493, 1507
AjnI CCWGG 7 cut(s) 781, 870, 1171, 1366, 1375, 1548, 1569
AluBI AGCT 7 cut(s) 118, 367, 413, 896, 1301, 1501, 1600
AluI AGCT 7 cut(s) 118, 367, 413, 896, 1301, 1501, 1600
Alw26I GTCTC 3 cut(s) 474, 1094, 1263
AlwI GGATC 4 cut(s) 196, 815, 828, 1581
Ama87I CYCGRG 2 cut(s) 1260, 1512
AoxI GGCC 6 cut(s) 73, 181, 541, 565, 641, 1086
ApaI GGGCCC 1 cut(s) 645
ApeKI GCWGC 6 cut(s) 84, 87, 591, 605, 919, 1498
ApoI RAATTY 1 cut(s) 496
AsiGI ACCGGT 2 cut(s) 67, 1056
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 3 cut(s) 34, 92, 216
AspS9I GGNCC 6 cut(s) 65, 641, 642, 1086, 1546, 1553
AsuHPI GGTGA 3 cut(s) 771, 1143, 1197
AvaI CYCGRG 2 cut(s) 1260, 1512
AvaII GGWCC 3 cut(s) 65, 1546, 1553
BaeGI GKGCMC 3 cut(s) 645, 903, 1164
BalI TGGCCA 2 cut(s) 183, 567
BamHI GGATCC 1 cut(s) 820
BanI GGYRCC 1 cut(s) 1161
BanII GRGCYC 4 cut(s) 645, 1202, 1246, 1267
BarI GAAGNNNNNNTAC 2 cut(s) 933, 965
BauI CACGAG 2 cut(s) 812, 1272
BbsI GAAGAC 1 cut(s) 1475
BbvI GCAGC 6 cut(s) 96, 99, 592, 603, 931, 1510
BccI CCATC 8 cut(s) 56, 331, 337, 557, 848, 961, 993, 1451
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
BciVI GTATCC 1 cut(s) 1078
BcoDI GTCTC 3 cut(s) 474, 1094, 1263
BfmI CTRYAG 3 cut(s) 27, 398, 1538
BfrI CTTAAG 1 cut(s) 575
BfuI GTATCC 1 cut(s) 1078
BglI GCCNNNNNGGC 1 cut(s) 81
BisI GCNGC 7 cut(s) 85, 88, 592, 606, 920, 1499, 1584
BlsI GCNGC 7 cut(s) 86, 89, 593, 607, 921, 1500, 1585
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
Bme18I GGWCC 3 cut(s) 65, 1546, 1553
BmeT110I CYCGRG 2 cut(s) 1260, 1512
BmgT120I GGNCC 6 cut(s) 65, 641, 642, 1086, 1546, 1553
BmrFI CCNGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
BmsI GCATC 4 cut(s) 85, 868, 1138, 1452
BoxI GACNNNNGTC 2 cut(s) 1255, 1289
BpiI GAAGAC 1 cut(s) 1475
BpmI CTGGAG 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 470
BsaI GGTCTC 1 cut(s) 474
BsaJI CCNNGG 3 cut(s) 76, 863, 1549
BsaWI WCCGGW 2 cut(s) 67, 1056
BsaXI ACNNNNNCTCC 2 cut(s) 1508, 1538
Bse118I RCCGGY 3 cut(s) 67, 210, 1056
Bse1I ACTGG 2 cut(s) 184, 1071
Bse3DI GCAATG 1 cut(s) 526
BseBI CCWGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
BseDI CCNNGG 3 cut(s) 76, 863, 1549
BseGI GGATG 5 cut(s) 67, 412, 840, 859, 1267
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 2 cut(s) 184, 1071
BseRI GAGGAG 1 cut(s) 1532
BseSI GKGCMC 3 cut(s) 645, 903, 1164
BseXI GCAGC 6 cut(s) 96, 99, 592, 603, 931, 1510
BseYI CCCAGC 1 cut(s) 638
BsgI GTGCAG 1 cut(s) 1517
Bsh1236I CGCG 1 cut(s) 34
BshFI GGCC 6 cut(s) 75, 183, 543, 567, 643, 1088
BshNI GGYRCC 1 cut(s) 1161
BshTI ACCGGT 2 cut(s) 67, 1056
BsiHKCI CYCGRG 2 cut(s) 1260, 1512
BsiSI CCGG 3 cut(s) 68, 211, 1057
BslFI GGGAC 2 cut(s) 806, 1445
BsmAI GTCTC 3 cut(s) 474, 1094, 1263
BsmFI GGGAC 2 cut(s) 806, 1445
BsmI GAATGC 1 cut(s) 813
BsnI GGCC 6 cut(s) 75, 183, 543, 567, 643, 1088
Bso31I GGTCTC 1 cut(s) 474
BsoBI CYCGRG 2 cut(s) 1260, 1512
Bsp120I GGGCCC 1 cut(s) 641
Bsp1286I GDGCHC 6 cut(s) 645, 903, 1164, 1202, 1246, 1267
Bsp143I GATC 4 cut(s) 201, 820, 1534, 1573
Bsp19I CCATGG 1 cut(s) 863
BspACI CCGC 5 cut(s) 750, 1165, 1315, 1556, 1584
BspANI GGCC 6 cut(s) 75, 183, 543, 567, 643, 1088
BspCNI CTCAG 1 cut(s) 838
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 1 cut(s) 18
BspMAI CTGCAG 1 cut(s) 1542
BspPI GGATC 4 cut(s) 196, 815, 828, 1581
BspQI GCTCTTC 2 cut(s) 690, 1207
BspT107I GGYRCC 1 cut(s) 1161
BspTI CTTAAG 1 cut(s) 575
BspTNI GGTCTC 1 cut(s) 474
BsrDI GCAATG 1 cut(s) 526
BsrFI RCCGGY 3 cut(s) 67, 210, 1056
BsrI ACTGG 2 cut(s) 184, 1071
BssAI RCCGGY 3 cut(s) 67, 210, 1056
BssECI CCNNGG 3 cut(s) 76, 863, 1549
BssMI GATC 4 cut(s) 201, 820, 1534, 1573
BssSI CACGAG 2 cut(s) 812, 1272
BssT1I CCWWGG 1 cut(s) 863
Bst2BI CACGAG 2 cut(s) 812, 1272
Bst2UI CCWGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
Bst4CI ACNGT 6 cut(s) 276, 455, 1030, 1105, 1124, 1221
Bst6I CTCTTC 7 cut(s) 234, 247, 690, 705, 768, 1207, 1571
BstAFI CTTAAG 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 171, 924, 1143
BstDEI CTNAG 3 cut(s) 545, 846, 892
BstDSI CCRYGG 2 cut(s) 76, 863
BstF5I GGATG 5 cut(s) 67, 412, 840, 859, 1267
BstFNI CGCG 1 cut(s) 34
BstHHI GCGC 3 cut(s) 34, 92, 216
BstKTI GATC 4 cut(s) 204, 823, 1537, 1576
BstMAI GTCTC 3 cut(s) 474, 1094, 1263
BstMBI GATC 4 cut(s) 201, 820, 1534, 1573
BstMWI GCNNNNNNNGC 4 cut(s) 81, 170, 1537, 1592
BstNI CCWGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
BstNSI RCATGY 1 cut(s) 173
BstPAI GACNNNNGTC 2 cut(s) 1255, 1289
BstSCI CCNGG 7 cut(s) 781, 870, 1171, 1366, 1375, 1548, 1569
BstSFI CTRYAG 3 cut(s) 27, 398, 1538
BstSLI GKGCMC 3 cut(s) 645, 903, 1164
BstUI CGCG 1 cut(s) 34
BstV1I GCAGC 6 cut(s) 96, 99, 592, 603, 931, 1510
BstV2I GAAGAC 1 cut(s) 1475
BstX2I RGATCY 3 cut(s) 201, 820, 1573
BstXI CCANNNNNNTGG 1 cut(s) 1427
BstYI RGATCY 3 cut(s) 201, 820, 1573
BsuI GTATCC 1 cut(s) 1078
BsuRI GGCC 6 cut(s) 75, 183, 543, 567, 643, 1088
BtgI CCRYGG 2 cut(s) 76, 863
BtsCI GGATG 5 cut(s) 67, 412, 840, 859, 1267
BtsIMutI CAGTG 1 cut(s) 1078
Cac8I GCNNGC 3 cut(s) 171, 924, 1143
CciI TCATGA 1 cut(s) 18
CfoI GCGC 3 cut(s) 34, 92, 216
Cfr10I RCCGGY 3 cut(s) 67, 210, 1056
Cfr13I GGNCC 6 cut(s) 65, 641, 642, 1086, 1546, 1553
CsiI ACCWGGT 1 cut(s) 781
Csp6I GTAC 2 cut(s) 451, 696
CspAI ACCGGT 2 cut(s) 67, 1056
CviAII CATG 7 cut(s) 19, 170, 229, 864, 1348, 1385, 1393
CviQI GTAC 2 cut(s) 451, 696
DdeI CTNAG 3 cut(s) 545, 846, 892
DpnI GATC 4 cut(s) 203, 822, 1536, 1575
DpnII GATC 4 cut(s) 201, 820, 1534, 1573
DraIII CACNNNGTG 1 cut(s) 785
EaeI YGGCCR 3 cut(s) 73, 181, 565
Eam1104I CTCTTC 7 cut(s) 234, 247, 690, 705, 768, 1207, 1571
EarI CTCTTC 7 cut(s) 234, 247, 690, 705, 768, 1207, 1571
Eco130I CCWWGG 1 cut(s) 863
Eco24I GRGCYC 4 cut(s) 645, 1202, 1246, 1267
Eco31I GGTCTC 1 cut(s) 474
Eco47I GGWCC 3 cut(s) 65, 1546, 1553
Eco57I CTGAAG 1 cut(s) 1238
Eco88I CYCGRG 2 cut(s) 1260, 1512
EcoRII CCWGG 7 cut(s) 781, 870, 1171, 1366, 1375, 1548, 1569
EcoT14I CCWWGG 1 cut(s) 863
EcoT22I ATGCAT 2 cut(s) 175, 1131
EcoT38I GRGCYC 4 cut(s) 645, 1202, 1246, 1267
ErhI CCWWGG 1 cut(s) 863
FaeI CATG 7 cut(s) 22, 173, 232, 867, 1351, 1388, 1396
FalI AAGNNNNNCTT 2 cut(s) 1485, 1517
FaqI GGGAC 2 cut(s) 806, 1445
FatI CATG 7 cut(s) 18, 169, 228, 863, 1347, 1384, 1392
FauI CCCGC 2 cut(s) 743, 1308
Fnu4HI GCNGC 7 cut(s) 85, 88, 592, 606, 920, 1499, 1584
FokI GGATG 5 cut(s) 74, 419, 827, 846, 1254
FriOI GRGCYC 4 cut(s) 645, 1202, 1246, 1267
Fsp4HI GCNGC 7 cut(s) 85, 88, 592, 606, 920, 1499, 1584
GlaI GCGC 3 cut(s) 33, 91, 215
GluI GCNGC 7 cut(s) 85, 88, 592, 606, 920, 1499, 1584
GsaI CCCAGC 1 cut(s) 642
GsuI CTGGAG 1 cut(s) 594
HaeIII GGCC 6 cut(s) 75, 183, 543, 567, 643, 1088
HapII CCGG 3 cut(s) 68, 211, 1057
HhaI GCGC 3 cut(s) 34, 92, 216
Hin1II CATG 7 cut(s) 22, 173, 232, 867, 1351, 1388, 1396
Hin6I GCGC 3 cut(s) 32, 90, 214
HinP1I GCGC 3 cut(s) 32, 90, 214
HincII GTYRAC 2 cut(s) 1382, 1399
HindII GTYRAC 2 cut(s) 1382, 1399
HindIII AAGCTT 2 cut(s) 116, 1299
HinfI GANTC 6 cut(s) 300, 509, 850, 995, 1109, 1436
HpaII CCGG 3 cut(s) 68, 211, 1057
HphI GGTGA 3 cut(s) 771, 1143, 1197
Hpy166II GTNNAC 3 cut(s) 1382, 1399, 1411
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 8 cut(s) 19, 52, 199, 611, 736, 1097, 1274, 1514
Hpy8I GTNNAC 3 cut(s) 1382, 1399, 1411
HpyAV CCTTC 3 cut(s) 382, 1178, 1382
HpyCH4III ACNGT 6 cut(s) 276, 455, 1030, 1105, 1124, 1221
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 170, 1537, 1592
HpyF3I CTNAG 3 cut(s) 545, 846, 892
Hsp92II CATG 7 cut(s) 22, 173, 232, 867, 1351, 1388, 1396
HspAI GCGC 3 cut(s) 32, 90, 214
Kzo9I GATC 4 cut(s) 201, 820, 1534, 1573
LguI GCTCTTC 2 cut(s) 690, 1207
LmnI GCTCC 2 cut(s) 364, 613
Lsp1109I GCAGC 6 cut(s) 96, 99, 592, 603, 931, 1510
LweI GCATC 4 cut(s) 85, 868, 1138, 1452
MabI ACCWGGT 1 cut(s) 781
MaeIII GTNAC 4 cut(s) 416, 455, 777, 887
MalI GATC 4 cut(s) 203, 822, 1536, 1575
MboI GATC 4 cut(s) 201, 820, 1534, 1573
MflI RGATCY 3 cut(s) 201, 820, 1573
MhlI GDGCHC 6 cut(s) 645, 903, 1164, 1202, 1246, 1267
MlsI TGGCCA 2 cut(s) 183, 567
MluCI AATT 6 cut(s) 109, 159, 496, 598, 741, 1310
MluNI TGGCCA 2 cut(s) 183, 567
MlyI GAGTC 1 cut(s) 294
MmeI TCCRAC 1 cut(s) 1304
Mox20I TGGCCA 2 cut(s) 183, 567
Mph1103I ATGCAT 2 cut(s) 175, 1131
MroXI GAANNNNTTC 1 cut(s) 720
MscI TGGCCA 2 cut(s) 183, 567
MseI TTAA 8 cut(s) 176, 207, 269, 576, 744, 797, 1296, 1612
MslI CAYNNNNRTG 3 cut(s) 233, 1134, 1272
Msp20I TGGCCA 2 cut(s) 183, 567
MspCI CTTAAG 1 cut(s) 575
MspI CCGG 3 cut(s) 68, 211, 1057
MspR9I CCNGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
Mva1269I GAATGC 1 cut(s) 813
MvaI CCWGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
MvnI CGCG 1 cut(s) 34
MwoI GCNNNNNNNGC 4 cut(s) 81, 170, 1537, 1592
NcoI CCATGG 1 cut(s) 863
NdeII GATC 4 cut(s) 201, 820, 1534, 1573
NlaIII CATG 7 cut(s) 22, 173, 232, 867, 1351, 1388, 1396
NmeAIII GCCGAG 1 cut(s) 1114
NmuCI GTSAC 2 cut(s) 455, 777
NsiI ATGCAT 2 cut(s) 175, 1131
NspI RCATGY 1 cut(s) 173
PaeI GCATGC 1 cut(s) 173
PagI TCATGA 1 cut(s) 18
PciSI GCTCTTC 2 cut(s) 690, 1207
PctI GAATGC 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 5 cut(s) 509, 850, 995, 1109, 1436
PfoI TCCNGGA 2 cut(s) 1171, 1569
PinAI ACCGGT 2 cut(s) 67, 1056
PkrI GCNGC 7 cut(s) 86, 89, 593, 607, 921, 1500, 1585
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
PshAI GACNNNNGTC 2 cut(s) 1255, 1289
Psp6I CCWGG 7 cut(s) 781, 870, 1171, 1366, 1375, 1548, 1569
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 7 cut(s) 781, 870, 1171, 1366, 1375, 1548, 1569
PspOMI GGGCCC 1 cut(s) 641
PspPI GGNCC 6 cut(s) 65, 641, 642, 1086, 1546, 1553
PstI CTGCAG 1 cut(s) 1542
PsuI RGATCY 3 cut(s) 201, 820, 1573
RsaI GTAC 2 cut(s) 452, 697
RsaNI GTAC 2 cut(s) 451, 696
RseI CAYNNNNRTG 3 cut(s) 233, 1134, 1272
SapI GCTCTTC 2 cut(s) 690, 1207
SaqAI TTAA 8 cut(s) 176, 207, 269, 576, 744, 797, 1296, 1612
SatI GCNGC 7 cut(s) 85, 88, 592, 606, 920, 1499, 1584
Sau3AI GATC 4 cut(s) 201, 820, 1534, 1573
Sau96I GGNCC 6 cut(s) 65, 641, 642, 1086, 1546, 1553
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 7 cut(s) 783, 872, 1173, 1368, 1377, 1550, 1571
SduI GDGCHC 6 cut(s) 645, 903, 1164, 1202, 1246, 1267
SexAI ACCWGGT 1 cut(s) 781
SfaNI GCATC 4 cut(s) 85, 868, 1138, 1452
SfcI CTRYAG 3 cut(s) 27, 398, 1538
SinI GGWCC 3 cut(s) 65, 1546, 1553
SmiMI CAYNNNNRTG 3 cut(s) 233, 1134, 1272
SmlI CTYRAG 2 cut(s) 485, 575
SmoI CTYRAG 2 cut(s) 485, 575
SphI GCATGC 1 cut(s) 173
Sse9I AATT 6 cut(s) 109, 159, 496, 598, 741, 1310
SsiI CCGC 5 cut(s) 750, 1165, 1315, 1556, 1584
SspI AATATT 2 cut(s) 267, 553
StyD4I CCNGG 7 cut(s) 781, 870, 1171, 1366, 1375, 1548, 1569
StyI CCWWGG 1 cut(s) 863
TaaI ACNGT 6 cut(s) 276, 455, 1030, 1105, 1124, 1221
TaqII GACCGA 1 cut(s) 1301
TasI AATT 6 cut(s) 109, 159, 496, 598, 741, 1310
TatI WGTACW 1 cut(s) 450
TauI GCSGC 1 cut(s) 1586
TfiI GAWTC 5 cut(s) 509, 850, 995, 1109, 1436
Tru1I TTAA 8 cut(s) 176, 207, 269, 576, 744, 797, 1296, 1612
Tru9I TTAA 8 cut(s) 176, 207, 269, 576, 744, 797, 1296, 1612
TscAI CASTG 1 cut(s) 1078
TseFI GTSAC 2 cut(s) 455, 777
TseI GCWGC 6 cut(s) 84, 87, 591, 605, 919, 1498
Tsp45I GTSAC 2 cut(s) 455, 777
TspDTI ATGAA 9 cut(s) 35, 423, 464, 729, 842, 954, 1364, 1401, 1481
TspRI CASTG 1 cut(s) 1078
Vha464I CTTAAG 1 cut(s) 575
VpaK11BI GGWCC 3 cut(s) 65, 1546, 1553
XapI RAATTY 1 cut(s) 496
XceI RCATGY 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 779
XmnI GAANNNNTTC 1 cut(s) 720
ZrmI AGTACT 1 cut(s) 452
Zsp2I ATGCAT 2 cut(s) 175, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.