MD17G1053700.v1.1

SNF1-related protein kinase catalytic subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005352
Physical Location & Seq
Reverse (-)
4171189 .. 4175804
4616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1053700.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGACTAAGAGGTATCTTTCAAAAATTAGGAAAATGGATGGACCTGTTGGCCGTGGTGGTAGCAGTGCAGATACTTATTTACCAAATTACAAGCTCGGAAAGACTCTTGGTATTGGTTCTTTTGGAAAGGTTAAAATTGCAGAGCATGCATTAACTGGACATAAAGTTGCTATCAAGATCCTTAACCGCCGCAAAATAAAGAACATGGAAATGGAAGAGAAAGTGAGAAGAGAAATCAAAATATTGAGGTTGTTTATGCATCCTCACATTATACGACTCTATGAGGTCATTGAAACACCATCCGACATTTATGTCGTGATGGAGTATGTGAAGTCTGGGGAGCTCTTTGATTATATAGTAGAGAAGGGTAGGCTGCAGGAAGATGAAGCTCGTAACTTTTTTCAACAGATAATATCTGGCGTAGAGTATTGTCACAGGAATATGGTTGTTCATAGAGATTTAAAGCCCGAAAATTTGCTTCTGGATTCCAAATGCAATGTGAAAATTGCTGATTTTGGTCTGAGCAACATAATGCGTGATGGCCATTTTCTCAAGACAAGTTGCGGCAGCCCGAATTATGCTGCTCCAGAGGTTATATCTGGCAAGCTGTACGCTGGGCCTGAAGTGGATGTGTGGAGTTGTGGTGTTATACTATATGCTCTTCTTTGTGGCACCCTTCCATTTGATGATGAAAATATTCCAAACTTGTTTAAGAAAATAAAGGGTGGGATATACACTCTTCCTAGTCATTTGTCACCTGGTGCAAGAGACTTGATTCCAAGAATGCTTGTAGTTGATCCAATGAAGCGAATGACCATTCCTGAGATTCGACATCATGCATGGTTCCAGGCTCATCTTCCTCGTTATTTAGCTGTTCCTCCACCAGACACCATGCAACAAGCAAAAAAGATTGATGAGGAAATTCTTCAGGAGGTAGTTAAGATGGGATTTGACAGGAACCATCTGGTTGAGTCTCTACGTAGTAGAGTACAGAATGAGGGAACAGTTGCATACTATTTGTTATTGGACAACCGTTTCCGTGTATCCAGTGGCTATCTTGGAGCTGAGTTTCAGGAGACTGTGGATTGTGGTTTCAATCGTATGCAGCAAAGTGAGACTGCTGCTTCACCTGTTGGGCACCGCCTTCCTGGATATATGGAGTATCAAGGAATGGGTTTTAAACCACAGTTCCCTGTTGAAAGGAAATGGGCTCTTGGACTTCAGTCTCGAGCACATCCTCGTGAAATAATGACAGAAGTCCTTAAAGCTTTACAAGAATTGCGGGTTTGTTGGAAGAAGATAGGACACTACAACATGAAGTGTAGGTGGGTTTTTGACAATACAGGTCAAAACGAAGGCATGATCGACAACCCTGTGCACGGTAACCATTATTTTGGAGATGAGTCCAGCATCATCGAAAATGATGGTGCTATGAAGATGCCAAATGTGGTCAAGTTTGAAGTGCAGCTTTTCAAAACTCGGGAGGAGAAGTACCTGCTTGATCTTCAGAGGGTTCAGGGTCCGCAGTTTCTCTTCTTGGATCTTTGTGCTGCTTTCCTTGCCCAACTTCGTGTACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0007584 GO:0008150 GO:0008152 GO:0009593 GO:0009594 GO:0009605 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010050 GO:0010150 GO:0010182 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051606 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080022 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
KA1 Pkinase UBA

Protein Analysis

527

Amino Acids

60.36

Weight (kDa)

8.88

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 30 - 282 3.6e-82 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 31 - 277 5e-41 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 130 - 230 6.2e-07 Kinase-like
UBA PF00627 304 - 340 3.2e-08 UBA/TS-N domain
KA1 PF02149 483 - 525 3.3e-13 Kinase associated domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 1503
AccB1I GGYRCC 2 cut(s) 671, 1137
AciI CCGC 6 cut(s) 187, 190, 564, 1141, 1282, 1523
AclWI GGATC 3 cut(s) 172, 791, 1548
AcoI YGGCCR 2 cut(s) 49, 541
AcsI RAATTY 2 cut(s) 472, 921
AcuI CTGAAG 4 cut(s) 642, 911, 1205, 1490
AdeI CACNNNGTG 1 cut(s) 761
AfaI GTAC 4 cut(s) 611, 990, 1493, 1575
AfiI CCNNNNNNNGG 1 cut(s) 1379
AgsI TTSAA 7 cut(s) 21, 293, 404, 1096, 1199, 1460, 1474
AjnI CCWGG 3 cut(s) 757, 846, 1147
AluBI AGCT 8 cut(s) 94, 343, 389, 607, 872, 1064, 1268, 1468
AluI AGCT 8 cut(s) 94, 343, 389, 607, 872, 1064, 1268, 1468
Alw21I GWGCWC 3 cut(s) 345, 1234, 1380
Alw26I GTCTC 5 cut(s) 762, 978, 1070, 1109, 1230
Alw44I GTGCAC 1 cut(s) 1376
AlwI GGATC 3 cut(s) 172, 791, 1548
Ama87I CYCGRG 2 cut(s) 1227, 1479
AoxI GGCC 3 cut(s) 49, 541, 617
ApaLI GTGCAC 1 cut(s) 1376
ApeKI GCWGC 7 cut(s) 373, 567, 581, 1105, 1121, 1465, 1550
ApoI RAATTY 2 cut(s) 472, 921
Asp700I GAANNNNTTC 2 cut(s) 696, 924
AspS9I GGNCC 3 cut(s) 41, 617, 1520
AsuHPI GGTGA 2 cut(s) 747, 1119
AvaI CYCGRG 2 cut(s) 1227, 1479
AvaII GGWCC 2 cut(s) 41, 1520
BaeGI GKGCMC 2 cut(s) 1140, 1380
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 2 cut(s) 671, 1137
BanII GRGCYC 2 cut(s) 345, 1213
BauI CACGAG 1 cut(s) 1239
Bbv12I GWGCWC 3 cut(s) 345, 1234, 1380
BbvI GCAGC 7 cut(s) 360, 568, 579, 1108, 1117, 1477, 1537
BccI CCATC 7 cut(s) 32, 307, 313, 533, 937, 969, 1418
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 3 cut(s) 759, 848, 1149
BciVI GTATCC 1 cut(s) 1054
BcoDI GTCTC 5 cut(s) 762, 978, 1070, 1109, 1230
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 1 cut(s) 374
BfuAI ACCTGC 1 cut(s) 1503
BfuI GTATCC 1 cut(s) 1054
BisI GCNGC 9 cut(s) 190, 374, 565, 568, 582, 1106, 1122, 1466, 1551
BlsI GCNGC 9 cut(s) 191, 375, 566, 569, 583, 1107, 1123, 1467, 1552
Bme1390I CCNGG 3 cut(s) 759, 848, 1149
Bme18I GGWCC 2 cut(s) 41, 1520
BmeT110I CYCGRG 2 cut(s) 1227, 1479
BmgT120I GGNCC 3 cut(s) 41, 617, 1520
BmiI GGNNCC 5 cut(s) 673, 845, 959, 1139, 1521
BmrFI CCNGG 3 cut(s) 759, 848, 1149
BmsI GCATC 3 cut(s) 268, 1419, 1428
BoxI GACNNNNGTC 2 cut(s) 1222, 1256
BpmI CTGGAG 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 536
BsaAI YACGTR 1 cut(s) 980
BsaJI CCNNGG 1 cut(s) 52
BsaXI ACNNNNNCTCC 4 cut(s) 628, 658, 1475, 1505
Bsc4I CCNNNNNNNGG 1 cut(s) 1379
Bse1I ACTGG 2 cut(s) 160, 1047
Bse3DI GCAATG 1 cut(s) 502
BseBI CCWGG 3 cut(s) 759, 848, 1149
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 5 cut(s) 43, 259, 299, 634, 1234
BseLI CCNNNNNNNGG 1 cut(s) 1379
BseMI GCAATG 1 cut(s) 502
BseMII CTCAG 3 cut(s) 512, 813, 1056
BseNI ACTGG 2 cut(s) 160, 1047
BseRI GAGGAG 1 cut(s) 1499
BseSI GKGCMC 2 cut(s) 1140, 1380
BseXI GCAGC 7 cut(s) 360, 568, 579, 1108, 1117, 1477, 1537
BseYI CCCAGC 1 cut(s) 614
BsgI GTGCAG 2 cut(s) 87, 1484
BshFI GGCC 3 cut(s) 51, 543, 619
BshNI GGYRCC 2 cut(s) 671, 1137
BsiHKAI GWGCWC 3 cut(s) 345, 1234, 1380
BsiHKCI CYCGRG 2 cut(s) 1227, 1479
BslI CCNNNNNNNGG 1 cut(s) 1379
BsmAI GTCTC 5 cut(s) 762, 978, 1070, 1109, 1230
BsmI GAATGC 1 cut(s) 789
BsnI GGCC 3 cut(s) 51, 543, 619
BsoBI CYCGRG 2 cut(s) 1227, 1479
Bsp1286I GDGCHC 5 cut(s) 345, 1140, 1213, 1234, 1380
Bsp143I GATC 5 cut(s) 177, 796, 1362, 1501, 1540
BspACI CCGC 6 cut(s) 187, 190, 564, 1141, 1282, 1523
BspANI GGCC 3 cut(s) 51, 543, 619
BspCNI CTCAG 3 cut(s) 513, 814, 1057
BspLI GGNNCC 5 cut(s) 673, 845, 959, 1139, 1521
BspMAI CTGCAG 1 cut(s) 378
BspMI ACCTGC 1 cut(s) 1503
BspPI GGATC 3 cut(s) 172, 791, 1548
BspQI GCTCTTC 1 cut(s) 666
BspT107I GGYRCC 2 cut(s) 671, 1137
BsrDI GCAATG 1 cut(s) 502
BsrI ACTGG 2 cut(s) 160, 1047
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 5 cut(s) 177, 796, 1362, 1501, 1540
BssSI CACGAG 1 cut(s) 1239
Bst2BI CACGAG 1 cut(s) 1239
Bst2UI CCWGG 3 cut(s) 759, 848, 1149
Bst4CI ACNGT 5 cut(s) 1006, 1034, 1081, 1188, 1382
Bst6I CTCTTC 5 cut(s) 210, 223, 666, 744, 1538
BstAPI GCANNNNNTGC 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 980
BstC8I GCNNGC 2 cut(s) 147, 605
BstDEI CTNAG 4 cut(s) 6, 521, 822, 1065
BstDSI CCRYGG 1 cut(s) 52
BstEII GGTNACC 1 cut(s) 1382
BstF5I GGATG 5 cut(s) 43, 259, 299, 634, 1234
BstKTI GATC 5 cut(s) 180, 799, 1365, 1504, 1543
BstMAI GTCTC 5 cut(s) 762, 978, 1070, 1109, 1230
BstMBI GATC 5 cut(s) 177, 796, 1362, 1501, 1540
BstMWI GCNNNNNNNGC 2 cut(s) 146, 1559
BstNI CCWGG 3 cut(s) 759, 848, 1149
BstNSI RCATGY 1 cut(s) 149
BstPAI GACNNNNGTC 2 cut(s) 1222, 1256
BstPI GGTNACC 1 cut(s) 1382
BstSCI CCNGG 3 cut(s) 757, 846, 1147
BstSFI CTRYAG 1 cut(s) 374
BstSLI GKGCMC 2 cut(s) 1140, 1380
BstSNI TACGTA 1 cut(s) 980
BstV1I GCAGC 7 cut(s) 360, 568, 579, 1108, 1117, 1477, 1537
BstX2I RGATCY 2 cut(s) 177, 1540
BstXI CCANNNNNNTGG 1 cut(s) 1394
BstYI RGATCY 2 cut(s) 177, 1540
BsuI GTATCC 1 cut(s) 1054
BsuRI GGCC 3 cut(s) 51, 543, 619
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 5 cut(s) 43, 259, 299, 634, 1234
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 1054
BveI ACCTGC 1 cut(s) 1503
Cac8I GCNNGC 2 cut(s) 147, 605
Cfr13I GGNCC 3 cut(s) 41, 617, 1520
CsiI ACCWGGT 1 cut(s) 757
Csp6I GTAC 4 cut(s) 610, 989, 1492, 1574
CviAII CATG 7 cut(s) 146, 205, 836, 840, 892, 1315, 1360
CviQI GTAC 4 cut(s) 610, 989, 1492, 1574
DdeI CTNAG 4 cut(s) 6, 521, 822, 1065
DpnI GATC 5 cut(s) 179, 798, 1364, 1503, 1542
DpnII GATC 5 cut(s) 177, 796, 1362, 1501, 1540
DraI TTTAAA 2 cut(s) 462, 1180
DraIII CACNNNGTG 1 cut(s) 761
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 2 cut(s) 49, 541
Eam1104I CTCTTC 5 cut(s) 210, 223, 666, 744, 1538
EarI CTCTTC 5 cut(s) 210, 223, 666, 744, 1538
Ecl136II GAGCTC 1 cut(s) 343
Eco105I TACGTA 1 cut(s) 980
Eco24I GRGCYC 2 cut(s) 345, 1213
Eco47I GGWCC 2 cut(s) 41, 1520
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 4 cut(s) 642, 911, 1205, 1490
Eco88I CYCGRG 2 cut(s) 1227, 1479
Eco91I GGTNACC 1 cut(s) 1382
EcoICRI GAGCTC 1 cut(s) 343
EcoO65I GGTNACC 1 cut(s) 1382
EcoRII CCWGG 3 cut(s) 757, 846, 1147
EcoT22I ATGCAT 3 cut(s) 151, 261, 841
EcoT38I GRGCYC 2 cut(s) 345, 1213
FaeI CATG 7 cut(s) 149, 208, 839, 843, 895, 1318, 1363
FalI AAGNNNNNCTT 2 cut(s) 1452, 1484
FatI CATG 7 cut(s) 145, 204, 835, 839, 891, 1314, 1359
FauI CCCGC 1 cut(s) 1275
Fnu4HI GCNGC 9 cut(s) 190, 374, 565, 568, 582, 1106, 1122, 1466, 1551
FokI GGATG 5 cut(s) 50, 246, 286, 641, 1221
FriOI GRGCYC 2 cut(s) 345, 1213
Fsp4HI GCNGC 9 cut(s) 190, 374, 565, 568, 582, 1106, 1122, 1466, 1551
FspBI CTAG 1 cut(s) 744
GluI GCNGC 9 cut(s) 190, 374, 565, 568, 582, 1106, 1122, 1466, 1551
GsaI CCCAGC 1 cut(s) 618
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 3 cut(s) 51, 543, 619
Hin1II CATG 7 cut(s) 149, 208, 839, 843, 895, 1318, 1363
HindIII AAGCTT 1 cut(s) 1266
HinfI GANTC 7 cut(s) 103, 276, 485, 775, 826, 971, 1403
HphI GGTGA 2 cut(s) 747, 1119
Hpy166II GTNNAC 2 cut(s) 1378, 1574
Hpy188I TCNGA 4 cut(s) 98, 304, 522, 1509
Hpy8I GTNNAC 2 cut(s) 1378, 1574
HpyAV CCTTC 4 cut(s) 358, 686, 1154, 1349
HpyCH4III ACNGT 5 cut(s) 1006, 1034, 1081, 1188, 1382
HpyCH4IV ACGT 1 cut(s) 979
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 1559
HpyF3I CTNAG 4 cut(s) 6, 521, 822, 1065
HpySE526I ACGT 1 cut(s) 979
Hsp92II CATG 7 cut(s) 149, 208, 839, 843, 895, 1318, 1363
Kzo9I GATC 5 cut(s) 177, 796, 1362, 1501, 1540
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 3 cut(s) 340, 589, 1061
Lsp1109I GCAGC 7 cut(s) 360, 568, 579, 1108, 1117, 1477, 1537
LweI GCATC 3 cut(s) 268, 1419, 1428
MabI ACCWGGT 1 cut(s) 757
MaeI CTAG 1 cut(s) 744
MaeII ACGT 1 cut(s) 979
MaeIII GTNAC 4 cut(s) 392, 431, 753, 1382
MalI GATC 5 cut(s) 179, 798, 1364, 1503, 1542
MboI GATC 5 cut(s) 177, 796, 1362, 1501, 1540
MflI RGATCY 2 cut(s) 177, 1540
MhlI GDGCHC 5 cut(s) 345, 1140, 1213, 1234, 1380
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 8 cut(s) 24, 85, 135, 472, 504, 574, 921, 1277
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 4 cut(s) 97, 270, 980, 1412
MmeI TCCRAC 2 cut(s) 327, 1271
Mox20I TGGCCA 1 cut(s) 543
Mph1103I ATGCAT 3 cut(s) 151, 261, 841
MroXI GAANNNNTTC 2 cut(s) 696, 924
MscI TGGCCA 1 cut(s) 543
MseI TTAA 8 cut(s) 132, 152, 183, 461, 711, 939, 1179, 1263
MslI CAYNNNNRTG 2 cut(s) 209, 1239
Msp20I TGGCCA 1 cut(s) 543
MspR9I CCNGG 3 cut(s) 759, 848, 1149
Mva1269I GAATGC 1 cut(s) 789
MvaI CCWGG 3 cut(s) 759, 848, 1149
MwoI GCNNNNNNNGC 2 cut(s) 146, 1559
NdeII GATC 5 cut(s) 177, 796, 1362, 1501, 1540
NlaIII CATG 7 cut(s) 149, 208, 839, 843, 895, 1318, 1363
NlaIV GGNNCC 5 cut(s) 673, 845, 959, 1139, 1521
NmuCI GTSAC 2 cut(s) 431, 753
NsiI ATGCAT 3 cut(s) 151, 261, 841
NspI RCATGY 1 cut(s) 149
PaeI GCATGC 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 1227
PciSI GCTCTTC 1 cut(s) 666
PctI GAATGC 1 cut(s) 789
PdmI GAANNNNTTC 2 cut(s) 696, 924
PfeI GAWTC 3 cut(s) 485, 775, 826
PfoI TCCNGGA 1 cut(s) 1147
PkrI GCNGC 9 cut(s) 191, 375, 566, 569, 583, 1107, 1123, 1467, 1552
PleI GAGTC 4 cut(s) 97, 270, 979, 1411
PpsI GAGTC 4 cut(s) 97, 270, 979, 1411
Ppu21I YACGTR 1 cut(s) 980
PshAI GACNNNNGTC 2 cut(s) 1222, 1256
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 3 cut(s) 757, 846, 1147
PspEI GGTNACC 1 cut(s) 1382
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 3 cut(s) 757, 846, 1147
PspN4I GGNNCC 5 cut(s) 673, 845, 959, 1139, 1521
PspPI GGNCC 3 cut(s) 41, 617, 1520
PstI CTGCAG 1 cut(s) 378
PsuI RGATCY 2 cut(s) 177, 1540
RsaI GTAC 4 cut(s) 611, 990, 1493, 1575
RsaNI GTAC 4 cut(s) 610, 989, 1492, 1574
RseI CAYNNNNRTG 2 cut(s) 209, 1239
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 666
SaqAI TTAA 8 cut(s) 132, 152, 183, 461, 711, 939, 1179, 1263
SatI GCNGC 9 cut(s) 190, 374, 565, 568, 582, 1106, 1122, 1466, 1551
Sau3AI GATC 5 cut(s) 177, 796, 1362, 1501, 1540
Sau96I GGNCC 3 cut(s) 41, 617, 1520
SchI GAGTC 4 cut(s) 97, 270, 980, 1412
ScrFI CCNGG 3 cut(s) 759, 848, 1149
SduI GDGCHC 5 cut(s) 345, 1140, 1213, 1234, 1380
SexAI ACCWGGT 1 cut(s) 757
SfaNI GCATC 3 cut(s) 268, 1419, 1428
SfcI CTRYAG 1 cut(s) 374
Sfr274I CTCGAG 1 cut(s) 1227
SinI GGWCC 2 cut(s) 41, 1520
SlaI CTCGAG 1 cut(s) 1227
SmiMI CAYNNNNRTG 2 cut(s) 209, 1239
SmlI CTYRAG 2 cut(s) 551, 1227
SmoI CTYRAG 2 cut(s) 551, 1227
SnaBI TACGTA 1 cut(s) 980
SphI GCATGC 1 cut(s) 149
Sse9I AATT 8 cut(s) 24, 85, 135, 472, 504, 574, 921, 1277
SsiI CCGC 6 cut(s) 187, 190, 564, 1141, 1282, 1523
SspI AATATT 2 cut(s) 243, 697
SspMI CTAG 1 cut(s) 744
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 3 cut(s) 757, 846, 1147
TaaI ACNGT 5 cut(s) 1006, 1034, 1081, 1188, 1382
TaiI ACGT 1 cut(s) 982
TaqI TCGA 4 cut(s) 829, 1228, 1365, 1416
TasI AATT 8 cut(s) 24, 85, 135, 472, 504, 574, 921, 1277
TatI WGTACW 2 cut(s) 988, 1573
TauI GCSGC 2 cut(s) 192, 567
TfiI GAWTC 3 cut(s) 485, 775, 826
Tru1I TTAA 8 cut(s) 132, 152, 183, 461, 711, 939, 1179, 1263
Tru9I TTAA 8 cut(s) 132, 152, 183, 461, 711, 939, 1179, 1263
TscAI CASTG 2 cut(s) 70, 1054
TseFI GTSAC 2 cut(s) 431, 753
TseI GCWGC 7 cut(s) 373, 567, 581, 1105, 1121, 1465, 1550
Tsp45I GTSAC 2 cut(s) 431, 753
TspDTI ATGAA 6 cut(s) 399, 440, 705, 818, 1331, 1448
TspGWI ACGGA 1 cut(s) 1028
TspRI CASTG 2 cut(s) 70, 1054
VneI GTGCAC 1 cut(s) 1376
VpaK11BI GGWCC 2 cut(s) 41, 1520
XapI RAATTY 2 cut(s) 472, 921
XceI RCATGY 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 1227
XmnI GAANNNNTTC 2 cut(s) 696, 924
XspI CTAG 1 cut(s) 744
Zsp2I ATGCAT 3 cut(s) 151, 261, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.