Rorug02G0528100

SNF1-related protein kinase catalytic subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005352
Physical Location & Seq
Forward (+)
65788259 .. 65789091
833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0528100.1

Sequence Viewer

Length: 744 bp
ATGGAGCTCCAAACCCCGCAACAAATGGAACCACAACAACAGCAACCAACAGAGGAGGGAGGAAACACCCAAGCAGCTGGAAGTAGTGCCAACATGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGAACAAATAAGAATCCTCAAGGATCTTTACTACGTCAAGGGATTTAAGTGCCCAACTACAGAGCACATTCACGAGATCTGCCTCCAGCTGAACCAGTATGGACATGTTGAGGGTAAGAACATTTACTTTTGGTTCCAGAACGTCAGGGCTCGAGAGAAGCAGATGAATAGGTGTAAACAGGCTGCTCCAGTGCCCATGGAAACTAGTTCTCTTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGATGATGGTAGATCCATTGATCTCAACTTTGGTTCCACCTATTCTACTGGAGGACAAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGGTTCAAACTCTTCCTCTGTTCCCCGTGCACGGCGAGAACGTCTTTGGTAACCTGAAGGCTACTTCCGAGGAAGGTAGCGCTTTTGGTTACTATTCTGGTGGCTCAGGCGGTTACCACAGTGGCTCAAACGTTTCTCTTGAGCTCAGCCTAAATCCATCCGGAGCTGCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0007584 GO:0008150 GO:0008152 GO:0009593 GO:0009594 GO:0009605 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010050 GO:0010150 GO:0010182 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051606 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080022 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
KA1 Pkinase UBA

Protein Analysis

247

Amino Acids

26.6

Weight (kDa)

5.11

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 38 - 99 2.4e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 437
AccIII TCCGGA 1 cut(s) 728
AciI CCGC 2 cut(s) 17, 678
AclI AACGTT 1 cut(s) 699
AclWI GGATC 8 cut(s) 165, 354, 367, 402, 415, 435, 448, 483
AcuI CTGAAG 1 cut(s) 644
AfaI GTAC 2 cut(s) 115, 352
AfeI AGCGCT 1 cut(s) 649
AfiI CCNNNNNNNGG 2 cut(s) 437, 599
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 1 cut(s) 575
AhlI ACTAGT 2 cut(s) 338, 467
AjnI CCWGG 2 cut(s) 116, 564
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AluBI AGCT 6 cut(s) 7, 77, 223, 434, 712, 734
AluI AGCT 6 cut(s) 7, 77, 223, 434, 712, 734
Alw21I GWGCWC 4 cut(s) 9, 201, 600, 714
Alw44I GTGCAC 1 cut(s) 596
AlwI GGATC 8 cut(s) 165, 354, 367, 402, 415, 435, 448, 483
Ama87I CYCGRG 1 cut(s) 285
Aor13HI TCCGGA 1 cut(s) 728
Aor51HI AGCGCT 1 cut(s) 649
AoxI GGCC 1 cut(s) 428
ApaLI GTGCAC 1 cut(s) 596
ApeKI GCWGC 3 cut(s) 74, 317, 734
ArsI GACNNNNNNTTYG 4 cut(s) 407, 439, 440, 472
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 3 cut(s) 381, 428, 462
AsuHPI GGTGA 1 cut(s) 554
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 2 cut(s) 381, 462
BaeGI GKGCMC 3 cut(s) 188, 330, 600
BamHI GGATCC 3 cut(s) 359, 407, 440
BanII GRGCYC 3 cut(s) 9, 286, 714
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 4 cut(s) 9, 201, 600, 714
BbvI GCAGC 3 cut(s) 86, 304, 721
BccI CCATC 3 cut(s) 419, 476, 733
BceAI ACGGC 2 cut(s) 96, 616
BciT130I CCWGG 2 cut(s) 118, 566
BcuI ACTAGT 2 cut(s) 338, 467
BfaI CTAG 3 cut(s) 339, 468, 742
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 651
BglII AGATCT 1 cut(s) 210
BisI GCNGC 3 cut(s) 75, 318, 735
BlpI GCTNAGC 1 cut(s) 713
BlsI GCNGC 3 cut(s) 76, 319, 736
Bme1390I CCNGG 2 cut(s) 118, 566
Bme18I GGWCC 2 cut(s) 381, 462
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 381, 428, 462
BmiI GGNNCC 8 cut(s) 30, 269, 361, 382, 409, 442, 463, 511
BmrFI CCNGG 2 cut(s) 118, 566
BpmI CTGGAG 4 cut(s) 117, 203, 306, 546
Bpu10I CCTNAGC 1 cut(s) 673
Bpu1102I GCTNAGC 1 cut(s) 713
BpuEI CTTGAG 2 cut(s) 137, 728
BsaJI CCNNGG 2 cut(s) 330, 636
BsaWI WCCGGW 1 cut(s) 728
Bsc4I CCNNNNNNNGG 2 cut(s) 437, 599
Bse1I ACTGG 6 cut(s) 229, 323, 358, 391, 400, 529
BseAI TCCGGA 1 cut(s) 728
BseBI CCWGG 2 cut(s) 118, 566
BseDI CCNNGG 2 cut(s) 330, 636
BseGI GGATG 2 cut(s) 536, 725
BseLI CCNNNNNNNGG 2 cut(s) 437, 599
BseMII CTCAG 2 cut(s) 687, 727
BseNI ACTGG 6 cut(s) 229, 323, 358, 391, 400, 529
BseRI GAGGAG 3 cut(s) 68, 567, 570
BseSI GKGCMC 3 cut(s) 188, 330, 600
BseXI GCAGC 3 cut(s) 86, 304, 721
BshFI GGCC 1 cut(s) 430
BsiHKAI GWGCWC 4 cut(s) 9, 201, 600, 714
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 1 cut(s) 729
BslI CCNNNNNNNGG 2 cut(s) 437, 599
BsnI GGCC 1 cut(s) 430
BsoBI CYCGRG 1 cut(s) 285
Bsp1286I GDGCHC 7 cut(s) 9, 188, 201, 286, 330, 600, 714
Bsp13I TCCGGA 1 cut(s) 728
Bsp143I GATC 9 cut(s) 157, 210, 359, 367, 407, 440, 488, 496, 559
Bsp1720I GCTNAGC 1 cut(s) 713
Bsp19I CCATGG 1 cut(s) 330
BspACI CCGC 2 cut(s) 17, 678
BspANI GGCC 1 cut(s) 430
BspCNI CTCAG 2 cut(s) 686, 726
BspEI TCCGGA 1 cut(s) 728
BspLI GGNNCC 8 cut(s) 30, 269, 361, 382, 409, 442, 463, 511
BspPI GGATC 8 cut(s) 165, 354, 367, 402, 415, 435, 448, 483
BsrI ACTGG 6 cut(s) 229, 323, 358, 391, 400, 529
BssECI CCNNGG 2 cut(s) 330, 636
BssMI GATC 9 cut(s) 157, 210, 359, 367, 407, 440, 488, 496, 559
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 330
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 118, 566
Bst4CI ACNGT 1 cut(s) 689
Bst6I CTCTTC 1 cut(s) 585
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 2 cut(s) 673, 713
BstDSI CCRYGG 1 cut(s) 330
BstEII GGTNACC 2 cut(s) 617, 680
BstF5I GGATG 2 cut(s) 536, 725
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 9 cut(s) 160, 213, 362, 370, 410, 443, 491, 499, 562
BstMBI GATC 9 cut(s) 157, 210, 359, 367, 407, 440, 488, 496, 559
BstNI CCWGG 2 cut(s) 118, 566
BstNSI RCATGY 1 cut(s) 242
BstPI GGTNACC 2 cut(s) 617, 680
BstSCI CCNGG 2 cut(s) 116, 564
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 3 cut(s) 188, 330, 600
BstV1I GCAGC 3 cut(s) 86, 304, 721
BstX2I RGATCY 6 cut(s) 157, 210, 359, 407, 440, 488
BstXI CCANNNNNNTGG 1 cut(s) 77
BstYI RGATCY 6 cut(s) 157, 210, 359, 407, 440, 488
BsuRI GGCC 1 cut(s) 430
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 536, 725
BtsIMutI CAGTG 3 cut(s) 330, 384, 694
Cac8I GCNNGC 1 cut(s) 432
CfoI GCGC 1 cut(s) 650
Cfr13I GGNCC 3 cut(s) 381, 428, 462
CsiI ACCWGGT 1 cut(s) 116
Csp6I GTAC 2 cut(s) 114, 351
CviAII CATG 3 cut(s) 94, 239, 331
CviQI GTAC 2 cut(s) 114, 351
DdeI CTNAG 2 cut(s) 673, 713
DpnI GATC 9 cut(s) 159, 212, 361, 369, 409, 442, 490, 498, 561
DpnII GATC 9 cut(s) 157, 210, 359, 367, 407, 440, 488, 496, 559
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
Ecl136II GAGCTC 2 cut(s) 7, 712
Eco130I CCWWGG 1 cut(s) 330
Eco24I GRGCYC 3 cut(s) 9, 286, 714
Eco47I GGWCC 2 cut(s) 381, 462
Eco47III AGCGCT 1 cut(s) 649
Eco53kI GAGCTC 2 cut(s) 7, 712
Eco57I CTGAAG 1 cut(s) 644
Eco88I CYCGRG 1 cut(s) 285
Eco91I GGTNACC 2 cut(s) 617, 680
EcoICRI GAGCTC 2 cut(s) 7, 712
EcoO65I GGTNACC 2 cut(s) 617, 680
EcoRII CCWGG 2 cut(s) 116, 564
EcoT14I CCWWGG 1 cut(s) 330
EcoT38I GRGCYC 3 cut(s) 9, 286, 714
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 3 cut(s) 97, 242, 334
FaiI YATR 4 cut(s) 95, 234, 240, 332
FatI CATG 3 cut(s) 93, 238, 330
FauI CCCGC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 75, 318, 735
FokI GGATG 2 cut(s) 523, 712
FriOI GRGCYC 3 cut(s) 9, 286, 714
Fsp4HI GCNGC 3 cut(s) 75, 318, 735
FspBI CTAG 3 cut(s) 339, 468, 742
GlaI GCGC 1 cut(s) 649
GluI GCNGC 3 cut(s) 75, 318, 735
GsuI CTGGAG 4 cut(s) 117, 203, 306, 546
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 1 cut(s) 430
HapII CCGG 1 cut(s) 729
HhaI GCGC 1 cut(s) 650
Hin1II CATG 3 cut(s) 97, 242, 334
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HinfI GANTC 2 cut(s) 124, 147
HpaII CCGG 1 cut(s) 729
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 4 cut(s) 311, 384, 465, 598
Hpy188I TCNGA 1 cut(s) 637
Hpy188III TCNNGA 6 cut(s) 134, 206, 271, 287, 707, 729
Hpy8I GTNNAC 4 cut(s) 311, 384, 465, 598
HpyAV CCTTC 2 cut(s) 619, 635
HpyCH4III ACNGT 1 cut(s) 689
HpyCH4IV ACGT 4 cut(s) 168, 276, 609, 699
HpyCH4V TGCA 2 cut(s) 544, 598
HpyF3I CTNAG 2 cut(s) 673, 713
HpySE526I ACGT 4 cut(s) 168, 276, 609, 699
Hsp92II CATG 3 cut(s) 97, 242, 334
HspAI GCGC 1 cut(s) 648
Kpn2I TCCGGA 1 cut(s) 728
Kzo9I GATC 9 cut(s) 157, 210, 359, 367, 407, 440, 488, 496, 559
LmnI GCTCC 4 cut(s) 4, 12, 325, 731
Lsp1109I GCAGC 3 cut(s) 86, 304, 721
MabI ACCWGGT 1 cut(s) 116
MaeI CTAG 3 cut(s) 339, 468, 742
MaeII ACGT 4 cut(s) 168, 276, 609, 699
MaeIII GTNAC 3 cut(s) 617, 656, 680
MalI GATC 9 cut(s) 159, 212, 361, 369, 409, 442, 490, 498, 561
MboI GATC 9 cut(s) 157, 210, 359, 367, 407, 440, 488, 496, 559
MboII GAAGA 1 cut(s) 572
MflI RGATCY 6 cut(s) 157, 210, 359, 407, 440, 488
MhlI GDGCHC 7 cut(s) 9, 188, 201, 286, 330, 600, 714
MluCI AATT 3 cut(s) 373, 421, 454
MroI TCCGGA 1 cut(s) 728
MseI TTAA 1 cut(s) 180
MspA1I CMGCKG 3 cut(s) 77, 223, 434
MspI CCGG 1 cut(s) 729
MspR9I CCNGG 2 cut(s) 118, 566
MvaI CCWGG 2 cut(s) 118, 566
NcoI CCATGG 1 cut(s) 330
NdeII GATC 9 cut(s) 157, 210, 359, 367, 407, 440, 488, 496, 559
NlaIII CATG 3 cut(s) 97, 242, 334
NlaIV GGNNCC 8 cut(s) 30, 269, 361, 382, 409, 442, 463, 511
NspI RCATGY 1 cut(s) 242
PaeR7I CTCGAG 1 cut(s) 285
PciI ACATGT 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PfeI GAWTC 2 cut(s) 124, 147
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 3 cut(s) 76, 319, 736
PscI ACATGT 1 cut(s) 238
Psp124BI GAGCTC 2 cut(s) 9, 714
Psp1406I AACGTT 1 cut(s) 699
Psp6I CCWGG 2 cut(s) 116, 564
PspEI GGTNACC 2 cut(s) 617, 680
PspGI CCWGG 2 cut(s) 116, 564
PspN4I GGNNCC 8 cut(s) 30, 269, 361, 382, 409, 442, 463, 511
PspPI GGNCC 3 cut(s) 381, 428, 462
PsuI RGATCY 6 cut(s) 157, 210, 359, 407, 440, 488
PvuII CAGCTG 3 cut(s) 77, 223, 434
RsaI GTAC 2 cut(s) 115, 352
RsaNI GTAC 2 cut(s) 114, 351
SacI GAGCTC 2 cut(s) 9, 714
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 3 cut(s) 75, 318, 735
Sau3AI GATC 9 cut(s) 157, 210, 359, 367, 407, 440, 488, 496, 559
Sau96I GGNCC 3 cut(s) 381, 428, 462
ScrFI CCNGG 2 cut(s) 118, 566
SduI GDGCHC 7 cut(s) 9, 188, 201, 286, 330, 600, 714
SexAI ACCWGGT 1 cut(s) 116
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 285
SinI GGWCC 2 cut(s) 381, 462
SlaI CTCGAG 1 cut(s) 285
SmlI CTYRAG 3 cut(s) 152, 285, 707
SmoI CTYRAG 3 cut(s) 152, 285, 707
SpeI ACTAGT 2 cut(s) 338, 467
Sse9I AATT 3 cut(s) 373, 421, 454
SsiI CCGC 2 cut(s) 17, 678
SspMI CTAG 3 cut(s) 339, 468, 742
SstI GAGCTC 2 cut(s) 9, 714
StyD4I CCNGG 2 cut(s) 116, 564
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 689
TaiI ACGT 4 cut(s) 171, 279, 612, 702
TaqI TCGA 2 cut(s) 286, 414
TasI AATT 3 cut(s) 373, 421, 454
TfiI GAWTC 2 cut(s) 124, 147
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 3 cut(s) 330, 391, 694
TseI GCWGC 3 cut(s) 74, 317, 734
TspDTI ATGAA 1 cut(s) 314
TspRI CASTG 3 cut(s) 330, 391, 694
Van91I CCANNNNNTGG 1 cut(s) 437
VneI GTGCAC 1 cut(s) 596
VpaK11BI GGWCC 2 cut(s) 381, 462
XceI RCATGY 1 cut(s) 242
XhoI CTCGAG 1 cut(s) 285
XspI CTAG 3 cut(s) 339, 468, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.