MD09G1056200.v1.1

SNF1-related protein kinase catalytic subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005352
Physical Location & Seq
Reverse (-)
3719820 .. 3723909
4090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1056200.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGTTACAGAAATTTGTGATTAGGAAAATGGATGGACCGGTTGGCCGTGGTGGCAGCAGTGCGGATACTTATTTACCAAATTACAAGCTCGGAAAGACTCTTGGTATTGGTTCTTTTGGAAAGGTTAAAATTGCAGAGCATGCATTAACTGGACATAAAGTTGCTATCAAGATCCTTAACCGCCGCAAGATAAAGAACTTGGAAATGGAAGAGAAAGTGAGAAGAGAAATCAAAATATTGAGATTGTTTATGCATCCTCACATTATACGGCTCTATGAGGTCATTGAAACACCATCTGACATTTATGTCGTGATGGAGTATGTGAAGTCCGGGGAGCTCTTTGATTATATAGTAGAGAAGGGTAGGCTGCAGGAAGATGAAGCTCGTAACTTTTTTCAACAGATAATATCTGGTGTAGAGTATTGTCACAGGAATATGGTTGTTCATAGAGATTTAAAGCCAGAAAATTTGCTCTTGGATTCCAAATGCAATGTGAAAATTGCTGATTTTGGTCTGAGCAACGTAATGCGTGATGGCCATTTTCTCAAGACAAGTTGTGGCAGCCCCAATTATGCTGCTCCAGAGGTTATTTCTGGCAAGCTGTACGCTGGGCCTGAAGTGGATGTGTGGAGTTGTGGTGTTATACTATATGCTCTTCTTTGTGGCACGCTTCCATTTGATGATGAAAATATTCCTAACTTGTTTAAGAAAATAAAGGGTGGGATATACACTCTTCCTAGTCATTTGTCACCTGGTGCAAGAGACTTGATTCCAAGAATGCTCGTAGTTGATCCAATGAAGCGAATGACCATCCCTGAGATTCGTCAGCATGCATGGTTCCAGGCTCATCTTCCTCGTTATTTAGCTGTTTCTCCACCAGACACCATGCAACAAGCAAAAAAGATTGATGAAGAAATTCTTCAGGAGGTAGTTAAGATGGGATTTGACAGGAACCTTCTGGTTGAGTCGCTTCGTGGTAGAGTACAAAATGAGGGAACAGTTGCATACTATTTGTTATTGGACAACCGTTTCCGTGTATCCAGTGGCTATCTTGGAGCTGAATTTCAGGAGACTGTGGATTGTGGTTTCAATCGTATGCAGCAAAGTGAGACTGCTGCTTCACCTGTTGGGCACCGCCTTCCTGGATATATGGAGTATCAAGGAATGGGTTTTAGACCACAGTTCCCTGTTGAAAGGAAATGGGCTCTTGGACTTCAGTCTCGAGCACATCCTCGTGAAATAATGACAGAAGTCCTTAAAGCTTTACAAGAATTGCAGGTGTGTTGGAAGAAGATAGGACACTACAACATGAAGTGTAGGTGGGTTGCTGGCACTCCAGGTCATAATGAAGGCATGATCGACAATTCTGTGCACAGTAACAATTATTTTGGAGATGAATCCAGCATCATCGAAAATGATGGTGTTATGAGGATGCCAAATGTGGTCAAATTTGAAGTACAGCTTTTTAAAACTCGGGAGGAGAAGTACCTGCTTGATCTTCAAAGGGTCCAAGGTCCGCAGTTTCTCTTCTTGGATCTTTGTGCCGCTTTCCTTGCCCAACTTCGCGTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0007584 GO:0008150 GO:0008152 GO:0009593 GO:0009594 GO:0009605 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010050 GO:0010150 GO:0010182 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051606 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080022 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
KA1 Pkinase UBA

Protein Analysis

525

Amino Acids

59.9

Weight (kDa)

8.74

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 28 - 280 4.1e-82 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 29 - 276 5.1e-41 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 128 - 228 9.3e-07 Kinase-like
UBA PF00627 302 - 338 8.2e-07 UBA/TS-N domain
KA1 PF02149 481 - 523 3.3e-13 Kinase associated domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1267
AasI GACNNNNNNGTC 1 cut(s) 305
Acc36I ACCTGC 2 cut(s) 1267, 1497
AccB1I GGYRCC 1 cut(s) 1131
AccII CGCG 1 cut(s) 1566
AciI CCGC 6 cut(s) 62, 181, 184, 1135, 1517, 1545
AclWI GGATC 3 cut(s) 166, 785, 1542
AcoI YGGCCR 2 cut(s) 43, 535
AcsI RAATTY 5 cut(s) 11, 466, 915, 1061, 1448
AcuI CTGAAG 3 cut(s) 636, 905, 1199
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 4 cut(s) 605, 984, 1458, 1487
AgeI ACCGGT 1 cut(s) 37
AgsI TTSAA 6 cut(s) 287, 398, 1090, 1193, 1454, 1502
AjnI CCWGG 4 cut(s) 751, 840, 1141, 1336
AluBI AGCT 8 cut(s) 88, 337, 383, 601, 866, 1058, 1262, 1462
AluI AGCT 8 cut(s) 88, 337, 383, 601, 866, 1058, 1262, 1462
Alw21I GWGCWC 3 cut(s) 339, 1228, 1374
Alw26I GTCTC 4 cut(s) 756, 1064, 1103, 1224
Alw44I GTGCAC 1 cut(s) 1370
AlwI GGATC 3 cut(s) 166, 785, 1542
Ama87I CYCGRG 2 cut(s) 1221, 1473
AoxI GGCC 3 cut(s) 43, 535, 611
ApaLI GTGCAC 1 cut(s) 1370
ApeKI GCWGC 6 cut(s) 54, 367, 561, 575, 1099, 1115
ApoI RAATTY 5 cut(s) 11, 466, 915, 1061, 1448
AsiGI ACCGGT 1 cut(s) 37
Asp700I GAANNNNTTC 3 cut(s) 690, 915, 918
AspS9I GGNCC 4 cut(s) 35, 611, 1507, 1514
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 2 cut(s) 741, 1113
AvaI CYCGRG 2 cut(s) 1221, 1473
AvaII GGWCC 3 cut(s) 35, 1507, 1514
BaeGI GKGCMC 2 cut(s) 1134, 1374
BalI TGGCCA 1 cut(s) 537
BanI GGYRCC 1 cut(s) 1131
BanII GRGCYC 2 cut(s) 339, 1207
BauI CACGAG 1 cut(s) 1233
Bbv12I GWGCWC 3 cut(s) 339, 1228, 1374
BbvI GCAGC 6 cut(s) 66, 354, 562, 573, 1102, 1111
BccI CCATC 7 cut(s) 26, 301, 307, 527, 818, 931, 1412
BceAI ACGGC 2 cut(s) 30, 284
BciT130I CCWGG 4 cut(s) 753, 842, 1143, 1338
BciVI GTATCC 2 cut(s) 58, 1048
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 4 cut(s) 756, 1064, 1103, 1224
BfaI CTAG 1 cut(s) 738
BfmI CTRYAG 1 cut(s) 368
BfuAI ACCTGC 2 cut(s) 1267, 1497
BfuI GTATCC 2 cut(s) 58, 1048
BglI GCCNNNNNGGC 1 cut(s) 51
BisI GCNGC 8 cut(s) 55, 184, 368, 562, 576, 1100, 1116, 1545
BlsI GCNGC 8 cut(s) 56, 185, 369, 563, 577, 1101, 1117, 1546
Bme1390I CCNGG 5 cut(s) 331, 753, 842, 1143, 1338
Bme18I GGWCC 3 cut(s) 35, 1507, 1514
BmeT110I CYCGRG 2 cut(s) 1221, 1473
BmgT120I GGNCC 4 cut(s) 35, 611, 1507, 1514
BmiI GGNNCC 4 cut(s) 839, 953, 1133, 1508
BmrFI CCNGG 5 cut(s) 331, 753, 842, 1143, 1338
BmsI GCATC 3 cut(s) 262, 1413, 1422
BoxI GACNNNNGTC 2 cut(s) 1216, 1250
BpmI CTGGAG 2 cut(s) 564, 1320
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 331
BsaJI CCNNGG 3 cut(s) 46, 330, 1510
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 4 cut(s) 622, 652, 1469, 1499
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 154, 1041
Bse3DI GCAATG 1 cut(s) 496
BseBI CCWGG 4 cut(s) 753, 842, 1143, 1338
BseDI CCNNGG 3 cut(s) 46, 330, 1510
BseGI GGATG 6 cut(s) 37, 253, 628, 810, 1228, 1437
BseMI GCAATG 1 cut(s) 496
BseMII CTCAG 2 cut(s) 506, 807
BseNI ACTGG 2 cut(s) 154, 1041
BseRI GAGGAG 1 cut(s) 1493
BseSI GKGCMC 2 cut(s) 1134, 1374
BseXI GCAGC 6 cut(s) 66, 354, 562, 573, 1102, 1111
BseYI CCCAGC 1 cut(s) 608
Bsh1236I CGCG 1 cut(s) 1566
BshFI GGCC 3 cut(s) 45, 537, 613
BshNI GGYRCC 1 cut(s) 1131
BshTI ACCGGT 1 cut(s) 37
BsiHKAI GWGCWC 3 cut(s) 339, 1228, 1374
BsiHKCI CYCGRG 2 cut(s) 1221, 1473
BsiSI CCGG 2 cut(s) 38, 330
BsmAI GTCTC 4 cut(s) 756, 1064, 1103, 1224
BsmI GAATGC 1 cut(s) 783
BsnI GGCC 3 cut(s) 45, 537, 613
BsoBI CYCGRG 2 cut(s) 1221, 1473
Bsp1286I GDGCHC 5 cut(s) 339, 1134, 1207, 1228, 1374
Bsp143I GATC 5 cut(s) 171, 790, 1356, 1495, 1534
BspACI CCGC 6 cut(s) 62, 181, 184, 1135, 1517, 1545
BspANI GGCC 3 cut(s) 45, 537, 613
BspCNI CTCAG 2 cut(s) 507, 808
BspFNI CGCG 1 cut(s) 1566
BspLI GGNNCC 4 cut(s) 839, 953, 1133, 1508
BspMAI CTGCAG 1 cut(s) 372
BspMI ACCTGC 2 cut(s) 1267, 1497
BspPI GGATC 3 cut(s) 166, 785, 1542
BspQI GCTCTTC 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 1131
BsrDI GCAATG 1 cut(s) 496
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 2 cut(s) 154, 1041
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 46, 330, 1510
BssMI GATC 5 cut(s) 171, 790, 1356, 1495, 1534
BssSI CACGAG 1 cut(s) 1233
BssT1I CCWWGG 1 cut(s) 1510
Bst2BI CACGAG 1 cut(s) 1233
Bst2UI CCWGG 4 cut(s) 753, 842, 1143, 1338
Bst4CI ACNGT 5 cut(s) 1000, 1028, 1075, 1182, 1376
Bst6I CTCTTC 5 cut(s) 204, 217, 660, 738, 1532
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 5 cut(s) 141, 599, 668, 831, 1330
BstDEI CTNAG 2 cut(s) 515, 816
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 6 cut(s) 37, 253, 628, 810, 1228, 1437
BstFNI CGCG 1 cut(s) 1566
BstKTI GATC 5 cut(s) 174, 793, 1359, 1498, 1537
BstMAI GTCTC 4 cut(s) 756, 1064, 1103, 1224
BstMBI GATC 5 cut(s) 171, 790, 1356, 1495, 1534
BstMWI GCNNNNNNNGC 3 cut(s) 51, 140, 1553
BstNI CCWGG 4 cut(s) 753, 842, 1143, 1338
BstNSI RCATGY 2 cut(s) 143, 833
BstPAI GACNNNNGTC 2 cut(s) 1216, 1250
BstSCI CCNGG 5 cut(s) 329, 751, 840, 1141, 1336
BstSFI CTRYAG 1 cut(s) 368
BstSLI GKGCMC 2 cut(s) 1134, 1374
BstUI CGCG 1 cut(s) 1566
BstV1I GCAGC 6 cut(s) 66, 354, 562, 573, 1102, 1111
BstX2I RGATCY 2 cut(s) 171, 1534
BstYI RGATCY 2 cut(s) 171, 1534
BsuI GTATCC 2 cut(s) 58, 1048
BsuRI GGCC 3 cut(s) 45, 537, 613
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 6 cut(s) 37, 253, 628, 810, 1228, 1437
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 2 cut(s) 64, 1048
BveI ACCTGC 2 cut(s) 1267, 1497
Cac8I GCNNGC 5 cut(s) 141, 599, 668, 831, 1330
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 4 cut(s) 35, 611, 1507, 1514
CseI GACGC 1 cut(s) 1555
CsiI ACCWGGT 1 cut(s) 751
Csp6I GTAC 4 cut(s) 604, 983, 1457, 1486
CspAI ACCGGT 1 cut(s) 37
CviAII CATG 6 cut(s) 140, 830, 834, 886, 1309, 1354
CviQI GTAC 4 cut(s) 604, 983, 1457, 1486
DdeI CTNAG 2 cut(s) 515, 816
DpnI GATC 5 cut(s) 173, 792, 1358, 1497, 1536
DpnII GATC 5 cut(s) 171, 790, 1356, 1495, 1534
DraI TTTAAA 2 cut(s) 456, 1468
DraIII CACNNNGTG 1 cut(s) 755
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 2 cut(s) 43, 535
Eam1104I CTCTTC 5 cut(s) 204, 217, 660, 738, 1532
EarI CTCTTC 5 cut(s) 204, 217, 660, 738, 1532
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 1510
Eco24I GRGCYC 2 cut(s) 339, 1207
Eco47I GGWCC 3 cut(s) 35, 1507, 1514
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 3 cut(s) 636, 905, 1199
Eco88I CYCGRG 2 cut(s) 1221, 1473
EcoICRI GAGCTC 1 cut(s) 337
EcoRII CCWGG 4 cut(s) 751, 840, 1141, 1336
EcoT14I CCWWGG 1 cut(s) 1510
EcoT22I ATGCAT 3 cut(s) 145, 255, 835
EcoT38I GRGCYC 2 cut(s) 339, 1207
ErhI CCWWGG 1 cut(s) 1510
FaeI CATG 6 cut(s) 143, 833, 837, 889, 1312, 1357
FalI AAGNNNNNCTT 4 cut(s) 903, 935, 1446, 1478
FatI CATG 6 cut(s) 139, 829, 833, 885, 1308, 1353
Fnu4HI GCNGC 8 cut(s) 55, 184, 368, 562, 576, 1100, 1116, 1545
FokI GGATG 6 cut(s) 44, 240, 635, 797, 1215, 1444
FriOI GRGCYC 2 cut(s) 339, 1207
Fsp4HI GCNGC 8 cut(s) 55, 184, 368, 562, 576, 1100, 1116, 1545
FspBI CTAG 1 cut(s) 738
GluI GCNGC 8 cut(s) 55, 184, 368, 562, 576, 1100, 1116, 1545
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 2 cut(s) 564, 1320
HaeIII GGCC 3 cut(s) 45, 537, 613
HapII CCGG 2 cut(s) 38, 330
HgaI GACGC 1 cut(s) 1555
Hin1II CATG 6 cut(s) 143, 833, 837, 889, 1312, 1357
HindIII AAGCTT 1 cut(s) 1260
HinfI GANTC 6 cut(s) 97, 479, 769, 820, 965, 1397
HpaII CCGG 2 cut(s) 38, 330
HphI GGTGA 2 cut(s) 741, 1113
Hpy166II GTNNAC 1 cut(s) 1372
Hpy188I TCNGA 3 cut(s) 92, 298, 516
Hpy188III TCNNGA 9 cut(s) 169, 310, 547, 581, 923, 1067, 1221, 1235, 1475
Hpy8I GTNNAC 1 cut(s) 1372
HpyAV CCTTC 4 cut(s) 352, 965, 1148, 1343
HpyCH4III ACNGT 5 cut(s) 1000, 1028, 1075, 1182, 1376
HpyCH4IV ACGT 1 cut(s) 522
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 140, 1553
HpyF3I CTNAG 2 cut(s) 515, 816
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 6 cut(s) 143, 833, 837, 889, 1312, 1357
Kzo9I GATC 5 cut(s) 171, 790, 1356, 1495, 1534
LguI GCTCTTC 1 cut(s) 660
LmnI GCTCC 3 cut(s) 334, 583, 1055
Lsp1109I GCAGC 6 cut(s) 66, 354, 562, 573, 1102, 1111
LweI GCATC 3 cut(s) 262, 1413, 1422
MabI ACCWGGT 1 cut(s) 751
MaeI CTAG 1 cut(s) 738
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 5 cut(s) 3, 386, 425, 747, 1376
MalI GATC 5 cut(s) 173, 792, 1358, 1497, 1536
MboI GATC 5 cut(s) 171, 790, 1356, 1495, 1534
MflI RGATCY 2 cut(s) 171, 1534
MhlI GDGCHC 5 cut(s) 339, 1134, 1207, 1228, 1374
MlsI TGGCCA 1 cut(s) 537
MluNI TGGCCA 1 cut(s) 537
MlyI GAGTC 2 cut(s) 91, 974
MmeI TCCRAC 1 cut(s) 1265
MnlI CCTC 9 cut(s) 267, 271, 577, 864, 919, 985, 1242, 1422, 1471
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 3 cut(s) 145, 255, 835
MroXI GAANNNNTTC 3 cut(s) 690, 915, 918
MscI TGGCCA 1 cut(s) 537
MseI TTAA 8 cut(s) 126, 146, 177, 455, 705, 933, 1257, 1467
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 537
MspI CCGG 2 cut(s) 38, 330
MspR9I CCNGG 5 cut(s) 331, 753, 842, 1143, 1338
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 4 cut(s) 753, 842, 1143, 1338
MvnI CGCG 1 cut(s) 1566
MwoI GCNNNNNNNGC 3 cut(s) 51, 140, 1553
NciI CCSGG 1 cut(s) 331
NdeII GATC 5 cut(s) 171, 790, 1356, 1495, 1534
NlaIII CATG 6 cut(s) 143, 833, 837, 889, 1312, 1357
NlaIV GGNNCC 4 cut(s) 839, 953, 1133, 1508
NmuCI GTSAC 2 cut(s) 425, 747
NsiI ATGCAT 3 cut(s) 145, 255, 835
NspI RCATGY 2 cut(s) 143, 833
PaeI GCATGC 2 cut(s) 143, 833
PaeR7I CTCGAG 1 cut(s) 1221
PaqCI CACCTGC 1 cut(s) 1267
PciSI GCTCTTC 1 cut(s) 660
PctI GAATGC 1 cut(s) 783
PdmI GAANNNNTTC 3 cut(s) 690, 915, 918
PfeI GAWTC 4 cut(s) 479, 769, 820, 1397
PfoI TCCNGGA 1 cut(s) 1141
PinAI ACCGGT 1 cut(s) 37
PkrI GCNGC 8 cut(s) 56, 185, 369, 563, 577, 1101, 1117, 1546
PleI GAGTC 2 cut(s) 91, 973
PpsI GAGTC 2 cut(s) 91, 973
PshAI GACNNNNGTC 2 cut(s) 1216, 1250
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 4 cut(s) 751, 840, 1141, 1336
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 4 cut(s) 751, 840, 1141, 1336
PspN4I GGNNCC 4 cut(s) 839, 953, 1133, 1508
PspPI GGNCC 4 cut(s) 35, 611, 1507, 1514
PstI CTGCAG 1 cut(s) 372
PsuI RGATCY 2 cut(s) 171, 1534
RsaI GTAC 4 cut(s) 605, 984, 1458, 1487
RsaNI GTAC 4 cut(s) 604, 983, 1457, 1486
RseI CAYNNNNRTG 1 cut(s) 1233
SacI GAGCTC 1 cut(s) 339
SapI GCTCTTC 1 cut(s) 660
SaqAI TTAA 8 cut(s) 126, 146, 177, 455, 705, 933, 1257, 1467
SatI GCNGC 8 cut(s) 55, 184, 368, 562, 576, 1100, 1116, 1545
Sau3AI GATC 5 cut(s) 171, 790, 1356, 1495, 1534
Sau96I GGNCC 4 cut(s) 35, 611, 1507, 1514
SchI GAGTC 2 cut(s) 91, 974
ScrFI CCNGG 5 cut(s) 331, 753, 842, 1143, 1338
SduI GDGCHC 5 cut(s) 339, 1134, 1207, 1228, 1374
SexAI ACCWGGT 1 cut(s) 751
SfaNI GCATC 3 cut(s) 262, 1413, 1422
SfcI CTRYAG 1 cut(s) 368
Sfr274I CTCGAG 1 cut(s) 1221
SinI GGWCC 3 cut(s) 35, 1507, 1514
SlaI CTCGAG 1 cut(s) 1221
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 2 cut(s) 545, 1221
SmoI CTYRAG 2 cut(s) 545, 1221
SphI GCATGC 2 cut(s) 143, 833
SsiI CCGC 6 cut(s) 62, 181, 184, 1135, 1517, 1545
SspI AATATT 2 cut(s) 237, 691
SspMI CTAG 1 cut(s) 738
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 5 cut(s) 329, 751, 840, 1141, 1336
StyI CCWWGG 1 cut(s) 1510
TaaI ACNGT 5 cut(s) 1000, 1028, 1075, 1182, 1376
TaiI ACGT 1 cut(s) 525
TaqI TCGA 3 cut(s) 1222, 1359, 1410
TatI WGTACW 2 cut(s) 982, 1456
TauI GCSGC 2 cut(s) 186, 1547
TfiI GAWTC 4 cut(s) 479, 769, 820, 1397
Tru1I TTAA 8 cut(s) 126, 146, 177, 455, 705, 933, 1257, 1467
Tru9I TTAA 8 cut(s) 126, 146, 177, 455, 705, 933, 1257, 1467
TscAI CASTG 2 cut(s) 64, 1048
TseFI GTSAC 2 cut(s) 425, 747
TseI GCWGC 6 cut(s) 54, 367, 561, 575, 1099, 1115
Tsp45I GTSAC 2 cut(s) 425, 747
TspDTI ATGAA 8 cut(s) 393, 434, 699, 812, 924, 1325, 1362, 1410
TspGWI ACGGA 1 cut(s) 1022
TspRI CASTG 2 cut(s) 64, 1048
VneI GTGCAC 1 cut(s) 1370
VpaK11BI GGWCC 3 cut(s) 35, 1507, 1514
XapI RAATTY 5 cut(s) 11, 466, 915, 1061, 1448
XceI RCATGY 2 cut(s) 143, 833
XhoI CTCGAG 1 cut(s) 1221
XmnI GAANNNNTTC 3 cut(s) 690, 915, 918
XspI CTAG 1 cut(s) 738
Zsp2I ATGCAT 3 cut(s) 145, 255, 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.