Rorug02G0528000

SNF1-related protein kinase catalytic subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005352
Physical Location & Seq
Reverse (-)
65773419 .. 65782912
9494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0528000.1

Sequence Viewer

Length: 771 bp
ATGACCGGCCTGGAAGAAGGGCTATACTTTGCGCAGTTTAACCTTAACGAGGTAGTTTCAGCATTTGGTGTTGGAAGAGTAATTATGTCAAAGGATGGTGACTATGGTGAAGGTGACATAGTGATCTGTCCTTCCACATCTTTTGCCGAGTACTGTGTGGTATCATCCCAGATTTTAAGAAAGGTGGAACCAAATTCTGGGATTCCATTGCCTGAATATCTCGGTTTGCTAGGGATACCTGGGTTGGCAGCGTGGGTGGGAATAGAGGTGATCGCGGAGCCAATAAAAGCAGGGTCGAATGTGTTCATCTCAGCCGCGGCCAGTGCCGTTGGGATTTATGCAGGGCAGTTGGCCAAGCTTAAAGGCTGTCGGGTAATTGGAAGTACTGGAACTGACGACAAGGTAAAGCTTGTCAAAGAGGAATTTGGATATGATGATGCCTTCAACTACCACACAGAGAAAGATTTCGATGCTGCTTTAACCAGGTATTTCCCAAATGGAATTGATATATATCTTGACAATGTTGGTGGTAAAATGCTCGAGGCTGTTCTCAACCATGTCAACAAGCATGCAAAGGTCTCCCTTTGTGGCATGATATCTGGCTACAATAAGGTATGGACTGAGAGGGAGGGAGTGAGGAATCTACTGAATCTGATCGGCAAACAAGTGAAGATGCAAGGGTACATGGTGGATTCATACTATGATAGTTTTGGAGATTTTGCAAAGGAGATGGATCGAGAGCCATCTAAAGGAAGGCAAGATTGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0007584 GO:0008150 GO:0008152 GO:0009593 GO:0009594 GO:0009605 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009755 GO:0009756 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010050 GO:0010150 GO:0010182 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051606 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080022 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418
Pfam Domains
Protein Families
KA1 Pkinase UBA

Protein Analysis

256

Amino Acids

27.96

Weight (kDa)

5.01

Isoelectric Point (pI)

26.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 109 - 235 7.7e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 33
AccB7I CCANNNNNTGG 1 cut(s) 197
AccII CGCG 2 cut(s) 275, 317
AciI CCGC 3 cut(s) 275, 315, 317
AclWI GGATC 1 cut(s) 741
AcoI YGGCCR 2 cut(s) 318, 351
AcsI RAATTY 2 cut(s) 193, 422
AfaI GTAC 3 cut(s) 152, 385, 683
AfiI CCNNNNNNNGG 3 cut(s) 49, 197, 749
AgsI TTSAA 1 cut(s) 445
AjnI CCWGG 3 cut(s) 9, 238, 482
AluBI AGCT 2 cut(s) 358, 409
AluI AGCT 2 cut(s) 358, 409
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 1 cut(s) 741
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 3 cut(s) 7, 318, 351
ApeKI GCWGC 2 cut(s) 248, 473
ApoI RAATTY 2 cut(s) 193, 422
Asp700I GAANNNNTTC 2 cut(s) 302, 464
AspLEI GCGC 1 cut(s) 34
AsuHPI GGTGA 4 cut(s) 110, 119, 125, 280
AvaI CYCGRG 1 cut(s) 539
BaeI ACNNNNGTAYC 2 cut(s) 227, 260
BalI TGGCCA 1 cut(s) 353
BbvI GCAGC 2 cut(s) 260, 460
BccI CCATC 3 cut(s) 89, 724, 751
BceAI ACGGC 1 cut(s) 311
BciT130I CCWGG 3 cut(s) 11, 240, 484
BciVI GTATCC 1 cut(s) 228
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 228
BisI GCNGC 4 cut(s) 249, 315, 318, 474
BlsI GCNGC 4 cut(s) 250, 316, 319, 475
BmcAI AGTACT 2 cut(s) 152, 385
Bme1390I CCNGG 3 cut(s) 11, 240, 484
BmeT110I CYCGRG 1 cut(s) 539
BmiI GGNNCC 2 cut(s) 189, 279
BmrFI CCNGG 3 cut(s) 11, 240, 484
BmsI GCATC 3 cut(s) 427, 460, 663
BsaBI GATNNNNATC 1 cut(s) 510
BsaI GGTCTC 1 cut(s) 583
BsaJI CCNNGG 2 cut(s) 239, 315
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 197, 749
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 2 cut(s) 321, 391
Bse3DI GCAATG 1 cut(s) 206
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 3 cut(s) 11, 240, 484
BseDI CCNNGG 2 cut(s) 239, 315
BseGI GGATG 2 cut(s) 100, 164
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 3 cut(s) 49, 197, 749
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 2 cut(s) 324, 612
BseNI ACTGG 2 cut(s) 321, 391
BseXI GCAGC 2 cut(s) 260, 460
Bsh1236I CGCG 2 cut(s) 275, 317
BshFI GGCC 3 cut(s) 9, 320, 353
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 1 cut(s) 6
BslI CCNNNNNNNGG 3 cut(s) 49, 197, 749
BsmAI GTCTC 1 cut(s) 583
BsnI GGCC 3 cut(s) 9, 320, 353
Bso31I GGTCTC 1 cut(s) 583
BsoBI CYCGRG 1 cut(s) 539
Bsp143I GATC 4 cut(s) 123, 270, 654, 733
BspACI CCGC 3 cut(s) 275, 315, 317
BspANI GGCC 3 cut(s) 9, 320, 353
BspCNI CTCAG 2 cut(s) 323, 613
BspFNI CGCG 2 cut(s) 275, 317
BspLI GGNNCC 2 cut(s) 189, 279
BspPI GGATC 1 cut(s) 741
BspTNI GGTCTC 1 cut(s) 583
BsrDI GCAATG 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 2 cut(s) 321, 391
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 2 cut(s) 239, 315
BssMI GATC 4 cut(s) 123, 270, 654, 733
Bst2UI CCWGG 3 cut(s) 11, 240, 484
Bst4CI ACNGT 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 570
BstDEI CTNAG 2 cut(s) 310, 621
BstDSI CCRYGG 1 cut(s) 315
BstENI CCTNNNNNAGG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 100, 164
BstFNI CGCG 2 cut(s) 275, 317
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 4 cut(s) 126, 273, 657, 736
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 4 cut(s) 123, 270, 654, 733
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstNI CCWGG 3 cut(s) 11, 240, 484
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 3 cut(s) 9, 238, 482
BstUI CGCG 2 cut(s) 275, 317
BstV1I GCAGC 2 cut(s) 260, 460
BsuI GTATCC 1 cut(s) 228
BsuRI GGCC 3 cut(s) 9, 320, 353
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 100, 164
BtsIMutI CAGTG 1 cut(s) 328
Cac8I GCNNGC 1 cut(s) 570
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 5
Cfr42I CCGCGG 1 cut(s) 318
CsiI ACCWGGT 1 cut(s) 482
Csp6I GTAC 3 cut(s) 151, 384, 682
CspCI CAANNNNNGTGG 4 cut(s) 124, 159, 508, 543
CviAII CATG 4 cut(s) 557, 569, 592, 685
CviQI GTAC 3 cut(s) 151, 384, 682
DdeI CTNAG 2 cut(s) 310, 621
DpnI GATC 4 cut(s) 125, 272, 656, 735
DpnII GATC 4 cut(s) 123, 270, 654, 733
EaeI YGGCCR 2 cut(s) 318, 351
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 583
Eco32I GATATC 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 539
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoRII CCWGG 3 cut(s) 9, 238, 482
EcoRV GATATC 1 cut(s) 597
FaeI CATG 4 cut(s) 560, 572, 595, 688
FatI CATG 4 cut(s) 556, 568, 591, 684
Fnu4HI GCNGC 4 cut(s) 249, 315, 318, 474
FokI GGATG 2 cut(s) 107, 151
Fsp4HI GCNGC 4 cut(s) 249, 315, 318, 474
FspBI CTAG 1 cut(s) 230
FspI TGCGCA 1 cut(s) 33
GlaI GCGC 1 cut(s) 33
GluI GCNGC 4 cut(s) 249, 315, 318, 474
HaeIII GGCC 3 cut(s) 9, 320, 353
HapII CCGG 1 cut(s) 6
HhaI GCGC 1 cut(s) 34
Hin1II CATG 4 cut(s) 560, 572, 595, 688
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 2 cut(s) 356, 407
HinfI GANTC 4 cut(s) 202, 640, 649, 692
HpaII CCGG 1 cut(s) 6
HphI GGTGA 4 cut(s) 110, 119, 125, 280
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 1 cut(s) 654
Hpy188III TCNNGA 2 cut(s) 515, 737
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 5 cut(s) 11, 104, 141, 451, 747
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4V TGCA 4 cut(s) 341, 572, 676, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 2 cut(s) 310, 621
Hsp92II CATG 4 cut(s) 560, 572, 595, 688
HspAI GCGC 1 cut(s) 32
KspI CCGCGG 1 cut(s) 318
Kzo9I GATC 4 cut(s) 123, 270, 654, 733
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 2 cut(s) 260, 460
LweI GCATC 3 cut(s) 427, 460, 663
MabI ACCWGGT 1 cut(s) 482
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 2 cut(s) 98, 113
MalI GATC 4 cut(s) 125, 272, 656, 735
MboI GATC 4 cut(s) 123, 270, 654, 733
MboII GAAGA 3 cut(s) 26, 87, 682
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 5 cut(s) 81, 193, 375, 422, 501
MluNI TGGCCA 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 7 cut(s) 43, 259, 412, 535, 618, 622, 630
Mox20I TGGCCA 1 cut(s) 353
MroXI GAANNNNTTC 2 cut(s) 302, 464
MscI TGGCCA 1 cut(s) 353
MseI TTAA 5 cut(s) 39, 45, 176, 360, 479
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 3 cut(s) 11, 240, 484
MvaI CCWGG 3 cut(s) 11, 240, 484
MvnI CGCG 2 cut(s) 275, 317
MwoI GCNNNNNNNGC 1 cut(s) 323
NdeII GATC 4 cut(s) 123, 270, 654, 733
NlaIII CATG 4 cut(s) 560, 572, 595, 688
NlaIV GGNNCC 2 cut(s) 189, 279
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 2 cut(s) 98, 113
NsbI TGCGCA 1 cut(s) 33
NspI RCATGY 1 cut(s) 572
PaeI GCATGC 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 539
PdmI GAANNNNTTC 2 cut(s) 302, 464
PfeI GAWTC 4 cut(s) 202, 640, 649, 692
PflMI CCANNNNNTGG 1 cut(s) 197
PkrI GCNGC 4 cut(s) 250, 316, 319, 475
Psp6I CCWGG 3 cut(s) 9, 238, 482
PspGI CCWGG 3 cut(s) 9, 238, 482
PspN4I GGNNCC 2 cut(s) 189, 279
PspXI VCTCGAGB 1 cut(s) 539
RsaI GTAC 3 cut(s) 152, 385, 683
RsaNI GTAC 3 cut(s) 151, 384, 682
SacII CCGCGG 1 cut(s) 318
SaqAI TTAA 5 cut(s) 39, 45, 176, 360, 479
SatI GCNGC 4 cut(s) 249, 315, 318, 474
Sau3AI GATC 4 cut(s) 123, 270, 654, 733
ScaI AGTACT 2 cut(s) 152, 385
ScrFI CCNGG 3 cut(s) 11, 240, 484
SexAI ACCWGGT 1 cut(s) 482
SfaNI GCATC 3 cut(s) 427, 460, 663
Sfr274I CTCGAG 1 cut(s) 539
Sfr303I CCGCGG 1 cut(s) 318
SgrBI CCGCGG 1 cut(s) 318
SlaI CTCGAG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
SphI GCATGC 1 cut(s) 572
Sse9I AATT 5 cut(s) 81, 193, 375, 422, 501
SsiI CCGC 3 cut(s) 275, 315, 317
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 3 cut(s) 9, 238, 482
TaaI ACNGT 1 cut(s) 155
TaqI TCGA 4 cut(s) 296, 468, 540, 736
TasI AATT 5 cut(s) 81, 193, 375, 422, 501
TatI WGTACW 2 cut(s) 150, 383
TauI GCSGC 2 cut(s) 317, 320
TfiI GAWTC 4 cut(s) 202, 640, 649, 692
Tru1I TTAA 5 cut(s) 39, 45, 176, 360, 479
Tru9I TTAA 5 cut(s) 39, 45, 176, 360, 479
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 2 cut(s) 98, 113
TseI GCWGC 2 cut(s) 248, 473
Tsp45I GTSAC 2 cut(s) 98, 113
TspDTI ATGAA 2 cut(s) 295, 684
TspRI CASTG 1 cut(s) 328
Van91I CCANNNNNTGG 1 cut(s) 197
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 2 cut(s) 193, 422
XceI RCATGY 1 cut(s) 572
XhoI CTCGAG 1 cut(s) 539
XmnI GAANNNNTTC 2 cut(s) 302, 464
XspI CTAG 1 cut(s) 230
ZrmI AGTACT 2 cut(s) 152, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.