AT5G43950

BEST Arabidopsis thaliana protein match is Plant protein of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007670
Physical Location & Seq
Reverse (-)
17686500 .. 17688668
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43950.1

Sequence Viewer

Length: 1701 bp
ATGTTTGGATGCAAATGTCTGTATTGGAACAATCTCAAAGAATATCCGCCATTGAAGGAACCTGAAACCTTCTCTCTCCCTGCTTCTCTTCCCCAGTGGCCTTCAGGTCAAGGCTTTGGTTTAGGAAGAATCAATCTAGGCGAATTAGAAGTCGCAGAGATCACAAGTTTTGAATTCGTATGGCGATACTGCTCTCGTCGAGATAACAAGAAGAGTGTTTCCTTCTACAAACCTGACAAATTACCTGAAGATTTCCATTGCTTAGGTCATTACTGTCAGTCTGATTCTCACCTCTTGAGAGGTTTTCTTCTTGTAGCAAGACAAGTTAATAAGTCTTCAGAGCCTGCACTTGTGCAACCACTCGATTATACATTGGTCTGGAGCTCAAATGATTTATCAGAAGAGCGTCAAAGCGAATCGTACGGTTACTTCTGGTTACCGCAGCCTCCTCAAGGGTATAAACCGATCGGATATTTGGTTACAACGAGTCCCGCGAAACCTGAATTGGATCAAGTTCGATGCGTTCGAGCTGATCTAACCGATAAGTGTGAAGCACATAAAGTCATAATCACCGCAATATCGGATTCTTTAAGCATTCCTATGTTTATATGGAAAACAAGACCATCCGACCGCGGAATGCGTGGCAAAGGAGTCTCAACAGGTACATTCTTCTGCACAACTCAATCCCCTGAAGAAGATCATCTCAGTACAATCGCGTGTCTAAAAAACCTCGATTCGAGTTTACACGCAATGCCAAACATCGAACAAATCCATGCTATGATTCAACACTATGGTCCAAGAGTTTACTTCCATCCTAATGAAGTCTACCTACCTTCTTCAGTCTCATGGTTCTTCAAAAACGGTGCACTCTTATGCAGCAATTCAAATTCCTCTGTTATCAACAATGAACCTATAGACGAAACCGGCTCGAATCTACCTCATGGCGGAACCAACGATAAGCGATATTGGATCGATTTACCGATCAACGATCAACAAAGACGCGAATTCATCAAACGAGGCGATCTCGAGAGCTCGAAGCTTTACGTTCACGTTAAGCCAGCATTTGGAGGAACGTTTACGGATCTTGCGTTTTGGATTTTTTGTCCGTTCAACGGACCAGCGACTCTGAAGCTAGGGCTTATGGATCTCTCGTTGGCTAAAACTGGTCAACACGTTTGTGATTGGGAACATTTCACAGTCAGAATCAGTAATTTCTCCGGCGAGCTTTACTCTATTTACTTCTCTCAACACAGTGGCGGCGAGTGGATCAAACCTGAAAATCTTGAATTCGTTGAAGGAAGTAACAAAGCTGTGGTGTACTCGTCGAAGAATGGTCACGCGAGCTTTTCTAAGTCTGGAATGTATTTACAAGGATCTGCTTTGCTTGGAATTGGGATTAGGAATGATTCAGCGAAGAGTGATTTGTTTGTTGATTCGAGTTTGAAGTATGAGATTGTTGCGGCGGAGTATCTCAGAGGAGCGGTGGTGGAGCCGCCGTGGTTAGGTTATATGAGAGAGTGGGGACCGAAGATTGTGTATAATTCGAGATCGGAGATTGAGAAGTTGAATGAACGATTGCCATGGAGGTTGAGATCTTGGGTTGATGCTGTGTTGAGGAAGATTCCGGTAGAATTATCCGGCGAGGAAGGACCGACGGGACCTAAGGAGAAGAATAATTGGTTTGGTGATGAGAGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

566

Amino Acids

64.29

Weight (kDa)

6.23

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 21 - 564 7.6e-269 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1064
AccBSI CCGCTC 1 cut(s) 1481
AccI GTMKAC 1 cut(s) 825
AccII CGCG 5 cut(s) 494, 633, 716, 1002, 1340
AclI AACGTT 1 cut(s) 1073
AclWI GGATC 6 cut(s) 516, 977, 1089, 1152, 1274, 1381
AcsI RAATTY 4 cut(s) 173, 886, 1004, 1286
AcuI CTGAAG 6 cut(s) 87, 267, 321, 711, 822, 1148
AfaI GTAC 4 cut(s) 422, 664, 709, 1319
AfiI CCNNNNNNNGG 5 cut(s) 452, 944, 1064, 1112, 1502
AflIII ACRYGT 1 cut(s) 1171
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AleI CACNNNNGTG 1 cut(s) 1176
AluBI AGCT 8 cut(s) 384, 530, 1032, 1039, 1132, 1225, 1310, 1344
AluI AGCT 8 cut(s) 384, 530, 1032, 1039, 1132, 1225, 1310, 1344
Alw21I GWGCWC 3 cut(s) 386, 868, 1034
Alw26I GTCTC 2 cut(s) 658, 847
Alw44I GTGCAC 1 cut(s) 864
AlwI GGATC 6 cut(s) 516, 977, 1089, 1152, 1274, 1381
AlwNI CAGNNNCTG 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 1025
AoxI GGCC 1 cut(s) 98
ApaLI GTGCAC 1 cut(s) 864
ApeKI GCWGC 2 cut(s) 442, 876
ApoI RAATTY 4 cut(s) 173, 886, 1004, 1286
ArsI GACNNNNNNTTYG 2 cut(s) 135, 167
AspS9I GGNCC 5 cut(s) 794, 1115, 1523, 1649, 1658
AsuHPI GGTGA 3 cut(s) 281, 562, 1697
AvaI CYCGRG 1 cut(s) 1025
AvaII GGWCC 5 cut(s) 794, 1115, 1523, 1649, 1658
AxyI CCTNAGG 1 cut(s) 1662
BaeGI GKGCMC 1 cut(s) 868
BanII GRGCYC 2 cut(s) 386, 1034
BarI GAAGNNNNNNTAC 4 cut(s) 413, 445, 813, 845
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 3 cut(s) 386, 868, 1034
BbvI GCAGC 2 cut(s) 454, 888
BccI CCATC 2 cut(s) 631, 819
BceAI ACGGC 1 cut(s) 1480
BcoDI GTCTC 2 cut(s) 658, 847
BfaI CTAG 2 cut(s) 137, 1133
BfmI CTRYAG 1 cut(s) 912
BglII AGATCT 1 cut(s) 1592
BisI GCNGC 5 cut(s) 443, 877, 1258, 1461, 1493
BlsI GCNGC 5 cut(s) 444, 878, 1259, 1462, 1494
Bme18I GGWCC 5 cut(s) 794, 1115, 1523, 1649, 1658
BmeT110I CYCGRG 1 cut(s) 1025
BmgT120I GGNCC 5 cut(s) 794, 1115, 1523, 1649, 1658
BmiI GGNNCC 5 cut(s) 60, 949, 1491, 1524, 1659
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 2 cut(s) 509, 1594
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 327
BplI GAGNNNNNCTC 4 cut(s) 1008, 1040, 1214, 1246
BpmI CTGGAG 1 cut(s) 400
Bpu10I CCTNAGC 1 cut(s) 262
BpuEI CTTGAG 2 cut(s) 316, 435
Bsa29I ATCGAT 1 cut(s) 972
BsaJI CCNNGG 3 cut(s) 631, 1496, 1580
BsaWI WCCGGW 1 cut(s) 1624
BsaXI ACNNNNNCTCC 4 cut(s) 1470, 1481, 1500, 1511
Bsc4I CCNNNNNNNGG 5 cut(s) 452, 944, 1064, 1112, 1502
Bse118I RCCGGY 1 cut(s) 923
Bse1I ACTGG 2 cut(s) 94, 1168
Bse21I CCTNAGG 1 cut(s) 1662
Bse3DI GCAATG 2 cut(s) 256, 756
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 3 cut(s) 631, 1496, 1580
BseGI GGATG 3 cut(s) 14, 623, 811
BseLI CCNNNNNNNGG 5 cut(s) 452, 944, 1064, 1112, 1502
BseMI GCAATG 2 cut(s) 256, 756
BseMII CTCAG 2 cut(s) 718, 1486
BseNI ACTGG 2 cut(s) 94, 1168
BseRI GAGGAG 2 cut(s) 438, 1491
BseSI GKGCMC 1 cut(s) 868
BseXI GCAGC 2 cut(s) 454, 888
BsgI GTGCAG 2 cut(s) 330, 658
Bsh1236I CGCG 5 cut(s) 494, 633, 716, 1002, 1340
Bsh1285I CGRYCG 2 cut(s) 468, 631
BshFI GGCC 1 cut(s) 100
BshVI ATCGAT 1 cut(s) 972
BsiEI CGRYCG 2 cut(s) 468, 631
BsiHKAI GWGCWC 3 cut(s) 386, 868, 1034
BsiHKCI CYCGRG 1 cut(s) 1025
BsiSI CCGG 4 cut(s) 924, 1218, 1625, 1638
BsiWI CGTACG 1 cut(s) 420
BslFI GGGAC 3 cut(s) 474, 1536, 1671
BslI CCNNNNNNNGG 5 cut(s) 452, 944, 1064, 1112, 1502
BsmAI GTCTC 2 cut(s) 658, 847
BsmFI GGGAC 3 cut(s) 474, 1536, 1671
BsmI GAATGC 2 cut(s) 594, 642
BsnI GGCC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 1025
Bsp1286I GDGCHC 3 cut(s) 386, 868, 1034
Bsp19I CCATGG 1 cut(s) 1580
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 2 cut(s) 717, 1485
BspDI ATCGAT 1 cut(s) 972
BspFNI CGCG 5 cut(s) 494, 633, 716, 1002, 1340
BspLI GGNNCC 5 cut(s) 60, 949, 1491, 1524, 1659
BspPI GGATC 6 cut(s) 516, 977, 1089, 1152, 1274, 1381
BspQI GCTCTTC 1 cut(s) 396
BsrBI CCGCTC 1 cut(s) 1481
BsrDI GCAATG 2 cut(s) 256, 756
BsrFI RCCGGY 1 cut(s) 923
BsrI ACTGG 2 cut(s) 94, 1168
BssAI RCCGGY 1 cut(s) 923
BssECI CCNNGG 3 cut(s) 631, 1496, 1580
BssT1I CCWWGG 1 cut(s) 1580
Bst4CI ACNGT 5 cut(s) 275, 425, 863, 1198, 1253
Bst6I CTCTTC 4 cut(s) 93, 206, 396, 1409
BstC8I GCNNGC 4 cut(s) 345, 1059, 1223, 1342
BstDEI CTNAG 5 cut(s) 262, 704, 1350, 1472, 1662
BstDSI CCRYGG 3 cut(s) 631, 1496, 1580
BstEII GGTNACC 1 cut(s) 435
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 3 cut(s) 14, 623, 811
BstFNI CGCG 5 cut(s) 494, 633, 716, 1002, 1340
BstMAI GTCTC 2 cut(s) 658, 847
BstMCI CGRYCG 2 cut(s) 468, 631
BstPI GGTNACC 1 cut(s) 435
BstSFI CTRYAG 1 cut(s) 912
BstSLI GKGCMC 1 cut(s) 868
BstUI CGCG 5 cut(s) 494, 633, 716, 1002, 1340
BstV1I GCAGC 2 cut(s) 454, 888
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 4 cut(s) 1081, 1144, 1373, 1592
BstYI RGATCY 4 cut(s) 1081, 1144, 1373, 1592
Bsu15I ATCGAT 1 cut(s) 972
Bsu36I CCTNAGG 1 cut(s) 1662
BsuRI GGCC 1 cut(s) 100
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 3 cut(s) 631, 1496, 1580
BtsCI GGATG 3 cut(s) 14, 623, 811
BtsIMutI CAGTG 2 cut(s) 101, 1258
Cac8I GCNNGC 4 cut(s) 345, 1059, 1223, 1342
CaiI CAGNNNCTG 1 cut(s) 344
Cfr10I RCCGGY 1 cut(s) 923
Cfr13I GGNCC 5 cut(s) 794, 1115, 1523, 1649, 1658
Cfr42I CCGCGG 1 cut(s) 634
ClaI ATCGAT 1 cut(s) 972
CseI GACGC 2 cut(s) 395, 1008
Csp6I GTAC 4 cut(s) 421, 663, 708, 1318
CviAII CATG 4 cut(s) 773, 846, 941, 1581
CviQI GTAC 4 cut(s) 421, 663, 708, 1318
DdeI CTNAG 5 cut(s) 262, 704, 1350, 1472, 1662
Eam1104I CTCTTC 4 cut(s) 93, 206, 396, 1409
EarI CTCTTC 4 cut(s) 93, 206, 396, 1409
EciI GGCGGA 3 cut(s) 36, 960, 1478
Ecl136II GAGCTC 2 cut(s) 384, 1032
Eco130I CCWWGG 1 cut(s) 1580
Eco24I GRGCYC 2 cut(s) 386, 1034
Eco47I GGWCC 5 cut(s) 794, 1115, 1523, 1649, 1658
Eco53kI GAGCTC 2 cut(s) 384, 1032
Eco57I CTGAAG 6 cut(s) 87, 267, 321, 711, 822, 1148
Eco81I CCTNAGG 1 cut(s) 1662
Eco88I CYCGRG 1 cut(s) 1025
Eco91I GGTNACC 1 cut(s) 435
EcoICRI GAGCTC 2 cut(s) 384, 1032
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoO109I RGGNCCY 1 cut(s) 1658
EcoO65I GGTNACC 1 cut(s) 435
EcoRI GAATTC 3 cut(s) 173, 1004, 1286
EcoT14I CCWWGG 1 cut(s) 1580
EcoT38I GRGCYC 2 cut(s) 386, 1034
ErhI CCWWGG 1 cut(s) 1580
FaeI CATG 4 cut(s) 776, 849, 944, 1584
FaqI GGGAC 3 cut(s) 474, 1536, 1671
FatI CATG 4 cut(s) 772, 845, 940, 1580
FauI CCCGC 1 cut(s) 499
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 5 cut(s) 443, 877, 1258, 1461, 1493
FokI GGATG 3 cut(s) 21, 610, 798
FriOI GRGCYC 2 cut(s) 386, 1034
Fsp4HI GCNGC 5 cut(s) 443, 877, 1258, 1461, 1493
FspBI CTAG 2 cut(s) 137, 1133
GluI GCNGC 5 cut(s) 443, 877, 1258, 1461, 1493
GsuI CTGGAG 1 cut(s) 400
HaeIII GGCC 1 cut(s) 100
HapII CCGG 4 cut(s) 924, 1218, 1625, 1638
HgaI GACGC 2 cut(s) 395, 1008
Hin1II CATG 4 cut(s) 776, 849, 944, 1584
HincII GTYRAC 1 cut(s) 1169
HindII GTYRAC 1 cut(s) 1169
HindIII AAGCTT 1 cut(s) 1037
HpaII CCGG 4 cut(s) 924, 1218, 1625, 1638
HphI GGTGA 3 cut(s) 281, 562, 1697
Hpy166II GTNNAC 8 cut(s) 743, 805, 826, 866, 1048, 1077, 1169, 1318
Hpy188III TCNNGA 8 cut(s) 200, 295, 379, 1025, 1027, 1283, 1356, 1545
Hpy8I GTNNAC 8 cut(s) 743, 805, 826, 866, 1048, 1077, 1169, 1318
Hpy99I CGWCG 3 cut(s) 201, 1327, 1657
HpyAV CCTTC 7 cut(s) 49, 79, 111, 232, 843, 1289, 1640
HpyCH4III ACNGT 5 cut(s) 275, 425, 863, 1198, 1253
HpyCH4IV ACGT 4 cut(s) 1044, 1050, 1073, 1173
HpyCH4V TGCA 6 cut(s) 12, 347, 355, 675, 866, 876
HpyF3I CTNAG 5 cut(s) 262, 704, 1350, 1472, 1662
HpySE526I ACGT 4 cut(s) 1044, 1050, 1073, 1173
Hsp92II CATG 4 cut(s) 776, 849, 944, 1584
KspI CCGCGG 1 cut(s) 634
LguI GCTCTTC 1 cut(s) 396
LmnI GCTCC 3 cut(s) 381, 1478, 1489
Lsp1109I GCAGC 2 cut(s) 454, 888
LweI GCATC 2 cut(s) 509, 1594
MaeI CTAG 2 cut(s) 137, 1133
MaeII ACGT 4 cut(s) 1044, 1050, 1073, 1173
MaeIII GTNAC 5 cut(s) 425, 435, 478, 1301, 1334
MbiI CCGCTC 1 cut(s) 1481
MflI RGATCY 4 cut(s) 1081, 1144, 1373, 1592
MhlI GDGCHC 3 cut(s) 386, 868, 1034
MlyI GAGTC 3 cut(s) 496, 660, 1117
MmeI TCCRAC 1 cut(s) 651
MseI TTAA 3 cut(s) 327, 590, 1053
MslI CAYNNNNRTG 4 cut(s) 599, 816, 871, 1176
MspA1I CMGCKG 1 cut(s) 633
MspI CCGG 4 cut(s) 924, 1218, 1625, 1638
Mva1269I GAATGC 2 cut(s) 594, 642
MvnI CGCG 5 cut(s) 494, 633, 716, 1002, 1340
NcoI CCATGG 1 cut(s) 1580
NlaIII CATG 4 cut(s) 776, 849, 944, 1584
NlaIV GGNNCC 5 cut(s) 60, 949, 1491, 1524, 1659
NmuCI GTSAC 1 cut(s) 1334
OliI CACNNNNGTG 1 cut(s) 1176
PaeR7I CTCGAG 1 cut(s) 1025
PciSI GCTCTTC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 3 cut(s) 491, 523, 1085
PctI GAATGC 2 cut(s) 594, 642
Pfl23II CGTACG 1 cut(s) 420
PflMI CCANNNNNTGG 1 cut(s) 1064
PkrI GCNGC 5 cut(s) 444, 878, 1259, 1462, 1494
Ple19I CGATCG 1 cut(s) 468
PleI GAGTC 3 cut(s) 495, 659, 1117
PpsI GAGTC 3 cut(s) 495, 659, 1117
PpuMI RGGWCCY 1 cut(s) 1658
Psp124BI GAGCTC 2 cut(s) 386, 1034
Psp1406I AACGTT 1 cut(s) 1073
Psp5II RGGWCCY 1 cut(s) 1658
PspEI GGTNACC 1 cut(s) 435
PspLI CGTACG 1 cut(s) 420
PspN4I GGNNCC 5 cut(s) 60, 949, 1491, 1524, 1659
PspPI GGNCC 5 cut(s) 794, 1115, 1523, 1649, 1658
PspPPI RGGWCCY 1 cut(s) 1658
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 4 cut(s) 1081, 1144, 1373, 1592
PvuI CGATCG 1 cut(s) 468
RsaI GTAC 4 cut(s) 422, 664, 709, 1319
RsaNI GTAC 4 cut(s) 421, 663, 708, 1318
RseI CAYNNNNRTG 4 cut(s) 599, 816, 871, 1176
SacI GAGCTC 2 cut(s) 386, 1034
SacII CCGCGG 1 cut(s) 634
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 3 cut(s) 327, 590, 1053
SatI GCNGC 5 cut(s) 443, 877, 1258, 1461, 1493
Sau96I GGNCC 5 cut(s) 794, 1115, 1523, 1649, 1658
SchI GAGTC 3 cut(s) 496, 660, 1117
SduI GDGCHC 3 cut(s) 386, 868, 1034
SfaNI GCATC 2 cut(s) 509, 1594
SfcI CTRYAG 1 cut(s) 912
Sfr274I CTCGAG 1 cut(s) 1025
Sfr303I CCGCGG 1 cut(s) 634
SgrBI CCGCGG 1 cut(s) 634
SinI GGWCC 5 cut(s) 794, 1115, 1523, 1649, 1658
SlaI CTCGAG 1 cut(s) 1025
SmiMI CAYNNNNRTG 4 cut(s) 599, 816, 871, 1176
SmlI CTYRAG 3 cut(s) 295, 450, 1025
SmoI CTYRAG 3 cut(s) 295, 450, 1025
SspMI CTAG 2 cut(s) 137, 1133
SstI GAGCTC 2 cut(s) 386, 1034
StyI CCWWGG 1 cut(s) 1580
TaaI ACNGT 5 cut(s) 275, 425, 863, 1198, 1253
TaiI ACGT 4 cut(s) 1047, 1053, 1076, 1176
TaqII GACCGA 2 cut(s) 1540, 1666
TatI WGTACW 2 cut(s) 707, 1317
TauI GCSGC 3 cut(s) 1260, 1463, 1495
Tru1I TTAA 3 cut(s) 327, 590, 1053
Tru9I TTAA 3 cut(s) 327, 590, 1053
TscAI CASTG 2 cut(s) 101, 1258
TseFI GTSAC 1 cut(s) 1334
TseI GCWGC 2 cut(s) 442, 876
Tsp45I GTSAC 1 cut(s) 1334
TspDTI ATGAA 4 cut(s) 834, 921, 997, 1584
TspGWI ACGGA 3 cut(s) 1094, 1095, 1128
TspRI CASTG 2 cut(s) 101, 1258
Van91I CCANNNNNTGG 1 cut(s) 1064
VneI GTGCAC 1 cut(s) 864
VpaK11BI GGWCC 5 cut(s) 794, 1115, 1523, 1649, 1658
XagI CCTNNNNNAGG 1 cut(s) 450
XapI RAATTY 4 cut(s) 173, 886, 1004, 1286
XhoI CTCGAG 1 cut(s) 1025
XmiI GTMKAC 1 cut(s) 825
XspI CTAG 2 cut(s) 137, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.