RchiOBHm_Chr6g0286661

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007670
Physical Location & Seq
Reverse (-)
50078852 .. 50083002
4151 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25712

Sequence Viewer

Length: 1704 bp
ATGTTCTTGTGCAAGTGCTTCTATTGGAATCAACTCAAAGATTTATTTCCACCAGAGCCTGAACCCTTCTCCCTGCCTGCTCCAATTCCTCAATGGCCTCCAGGTGAAGGTTTTGCTTCTGGAAAAATAAGTATTGGAGAAATAGAGGTTTCTAAGATCACCAGATTTGAACGAATCTGGGCCTGCTCTCTGCCAGAGGACAAGAAAAAAACAGTTACATTTTATAAGCCAGCAGGAATTCCTGATGGATTCCATAGCATGGGTCATTATTGCCAACTCAATGGCAAGCCTTTACACGGCTATGTTCTTGTGGTTCGAGAAGTAGATTTGCCAGAAACAGCTGGTGTTCAGGAGCATGTGAAGTCAGCAGCTCTTCGAGAGCCACTTGATTACACACTGGTGTGGAGCCCTGATGATGGGAATGAGGAAAGTTATGGTTCATGTGGCTACTTTTGGTTACCTGATCCACCCGAAGGTTACAGAGCAATGGGCTTTTTAGTTACCAACAAGCCAGGAAAACCTGCGTTGGATGAAGTAAGGTGTGTTAGAGCTGATTTGACTGACACATGCGAAACTCACAGCCTACTTTTTAATACCAGCATCAAATCTGCAAGTCTTCCTTTTCAAGTTTGGACTATGAGACCACGTCATCGGGGAATGATGGGGACAGGGGTCTCTGTAGGTACATTTTTCTGCAGTAGTCACTGGAGTAATGGAGAGGAACTGCAGATTGGGTGCTTGAAGAATCTGAATCCTAAGCTCTCTGGAATGCCAAATCTTGATCAAATTCATTCACTTATCAATCATTATGGACCCACTGTTTTCTTTCATCCAGAAGAGGTCTACTTGCCATCTTCTGTTGCCTGGTTCTTCAAAAATGGAGCGGTATTGTACAAAAGTGACACAGATGTAGGCGAAGCAATTGATGAAAGTGGCTCAAATTTGCCTGGTGGGGGGACAAATGATAAGCAGTTTTGGATAGACTTGCCTAGTGGTGATCGAAGAAAGAATGTGATATATGGAAACTTGGAAAGTGCAAAACTCTACGTTCATGTGAAACCAGCTCTTGGTGGGACTTTTACTGACATTGCAATGTGGGTATTTTGTCCCTTTAATGGACCAGCCACTCTCAAAGTCGGACCTATGGATATCGCTCTCAGCAAGATTGGACAGCATGTGAGTGACTGGGAGCATTTCACACTCCGAATATGCAACTTTACTGGAGAGCTTTGTAGTATATACTCCTCACAGCACAGCGGTGGTCAATGGGTAGATGCATATGCTTTGGAGTACATAAGCGGGAACAAAGCCATTATCTACTCGACGAAAAATGGACATGCAAGCTTCCCACATGCTGGGACTTACCTTCAGGGGTCTGCAAAGCTTGGGATTGGAATTAGGAATGATGCCGCTCGCAGTGAGTTTTCTGTGGATTCAAGCCGCCAATATGAAGTAGTTGCGGCTGAGTATCTTGGAGATGGAGTTGTTACTGAGCCAGGTTGGCTGCAGTTTATGAGAGAATGGGGTCCAAAAATTGTATACAATTCAAGGACTGAGCTGGATAAGATCATCAATGTTTTGCCTGTGATGTTGAGATATCCTGTGGAGAACATCTTTTCAAAAATTCCAGTTGAGCTTTCTGGGGAGGAAGGTCCTACAGGACCAAAGGAGAAGAACAATTGGGTGGGAGATGAAAGATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

567

Amino Acids

63.16

Weight (kDa)

5.63

Isoelectric Point (pI)

31.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 20 - 565 2.6e-264 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 529
AccB7I CCANNNNNTGG 3 cut(s) 259, 1067, 1355
AccBSI CCGCTC 2 cut(s) 884, 1412
AccI GTMKAC 2 cut(s) 843, 1539
AciI CCGC 6 cut(s) 884, 1257, 1299, 1410, 1441, 1460
AclWI GGATC 1 cut(s) 458
AcsI RAATTY 4 cut(s) 237, 786, 940, 1623
AcuI CTGAAG 1 cut(s) 1352
AfaI GTAC 3 cut(s) 685, 893, 1292
AfiI CCNNNNNNNGG 9 cut(s) 107, 259, 296, 416, 473, 953, 1067, 1115, 1355
AgsI TTSAA 7 cut(s) 170, 626, 742, 874, 1437, 1548, 1620
AjiI CACGTC 1 cut(s) 647
AjnI CCWGG 5 cut(s) 100, 511, 863, 946, 1495
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
AleI CACNNNNGTG 3 cut(s) 398, 400, 1257
Alw26I GTCTC 2 cut(s) 634, 679
AlwI GGATC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 59
AoxI GGCC 2 cut(s) 95, 180
ApeKI GCWGC 2 cut(s) 368, 1504
ApoI RAATTY 4 cut(s) 237, 786, 940, 1623
AspS9I GGNCC 7 cut(s) 180, 812, 1118, 1139, 1526, 1652, 1661
AsuHPI GGTGA 3 cut(s) 116, 151, 1007
AvaII GGWCC 6 cut(s) 812, 1118, 1139, 1526, 1652, 1661
BanII GRGCYC 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 608
BbvI GCAGC 2 cut(s) 380, 1491
BccI CCATC 6 cut(s) 239, 410, 655, 859, 1472, 1692
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 5 cut(s) 102, 513, 865, 948, 1497
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 2 cut(s) 634, 679
BfaI CTAG 1 cut(s) 990
BfmI CTRYAG 5 cut(s) 678, 694, 725, 1505, 1656
BfuAI ACCTGC 1 cut(s) 529
BglI GCCNNNNNGGC 1 cut(s) 1501
BisI GCNGC 5 cut(s) 369, 1410, 1441, 1461, 1505
BlsI GCNGC 5 cut(s) 370, 1411, 1442, 1462, 1506
Bme1390I CCNGG 5 cut(s) 102, 513, 865, 948, 1497
Bme18I GGWCC 6 cut(s) 812, 1118, 1139, 1526, 1652, 1661
BmgBI CACGTC 1 cut(s) 647
BmgT120I GGNCC 7 cut(s) 180, 812, 1118, 1139, 1526, 1652, 1661
BmiI GGNNCC 3 cut(s) 407, 814, 1527
BmrFI CCNGG 5 cut(s) 102, 513, 865, 948, 1497
BmrI ACTGGG 1 cut(s) 1195
BmsI GCATC 3 cut(s) 609, 1264, 1396
BmuI ACTGGG 1 cut(s) 1195
BoxI GACNNNNGTC 1 cut(s) 671
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 3 cut(s) 84, 727, 1242
Bpu10I CCTNAGC 1 cut(s) 756
BsaI GGTCTC 2 cut(s) 634, 679
BsaXI ACNNNNNCTCC 2 cut(s) 1467, 1497
Bsc4I CCNNNNNNNGG 9 cut(s) 107, 259, 296, 416, 473, 953, 1067, 1115, 1355
Bse1I ACTGG 5 cut(s) 402, 710, 1190, 1225, 1628
Bse3DI GCAATG 3 cut(s) 492, 1086, 1098
BseBI CCWGG 5 cut(s) 102, 513, 865, 948, 1497
BseGI GGATG 2 cut(s) 535, 829
BseLI CCNNNNNNNGG 9 cut(s) 107, 259, 296, 416, 473, 953, 1067, 1115, 1355
BseMI GCAATG 3 cut(s) 492, 1086, 1098
BseMII CTCAG 4 cut(s) 1171, 1455, 1482, 1545
BseNI ACTGG 5 cut(s) 402, 710, 1190, 1225, 1628
BseRI GAGGAG 1 cut(s) 1234
BseXI GCAGC 2 cut(s) 380, 1491
BseYI CCCAGC 1 cut(s) 1355
BshFI GGCC 2 cut(s) 97, 182
BslFI GGGAC 5 cut(s) 679, 970, 1087, 1092, 1372
BslI CCNNNNNNNGG 9 cut(s) 107, 259, 296, 416, 473, 953, 1067, 1115, 1355
BsmAI GTCTC 2 cut(s) 634, 679
BsmFI GGGAC 5 cut(s) 679, 970, 1087, 1092, 1372
BsmI GAATGC 1 cut(s) 774
BsnI GGCC 2 cut(s) 97, 182
Bso31I GGTCTC 2 cut(s) 634, 679
Bsp1286I GDGCHC 1 cut(s) 410
Bsp1407I TGTACA 1 cut(s) 891
Bsp143I GATC 5 cut(s) 156, 463, 781, 997, 1566
BspACI CCGC 6 cut(s) 884, 1257, 1299, 1410, 1441, 1460
BspANI GGCC 2 cut(s) 97, 182
BspCNI CTCAG 4 cut(s) 1170, 1456, 1483, 1546
BspLI GGNNCC 3 cut(s) 407, 814, 1527
BspMAI CTGCAG 3 cut(s) 698, 729, 1509
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 1 cut(s) 458
BspQI GCTCTTC 1 cut(s) 378
BspTNI GGTCTC 2 cut(s) 634, 679
BsrBI CCGCTC 2 cut(s) 884, 1412
BsrDI GCAATG 3 cut(s) 492, 1086, 1098
BsrGI TGTACA 1 cut(s) 891
BsrI ACTGG 5 cut(s) 402, 710, 1190, 1225, 1628
BssMI GATC 5 cut(s) 156, 463, 781, 997, 1566
BssNAI GTATAC 1 cut(s) 1540
Bst1107I GTATAC 1 cut(s) 1540
Bst2UI CCWGG 5 cut(s) 102, 513, 865, 948, 1497
Bst4CI ACNGT 2 cut(s) 214, 820
Bst6I CTCTTC 2 cut(s) 378, 831
BstAUI TGTACA 1 cut(s) 891
BstC8I GCNNGC 6 cut(s) 78, 184, 231, 287, 1342, 1414
BstDEI CTNAG 6 cut(s) 153, 756, 1157, 1464, 1491, 1554
BstEII GGTNACC 1 cut(s) 456
BstF5I GGATG 2 cut(s) 535, 829
BstKTI GATC 5 cut(s) 159, 466, 784, 1000, 1569
BstMAI GTCTC 2 cut(s) 634, 679
BstMBI GATC 5 cut(s) 156, 463, 781, 997, 1566
BstMWI GCNNNNNNNGC 1 cut(s) 1501
BstNI CCWGG 5 cut(s) 102, 513, 865, 948, 1497
BstNSI RCATGY 5 cut(s) 359, 570, 1178, 1340, 1355
BstPAI GACNNNNGTC 1 cut(s) 671
BstPI GGTNACC 1 cut(s) 456
BstSCI CCNGG 5 cut(s) 100, 511, 863, 946, 1495
BstSFI CTRYAG 5 cut(s) 678, 694, 725, 1505, 1656
BstV1I GCAGC 2 cut(s) 380, 1491
BstV2I GAAGAC 1 cut(s) 608
BstXI CCANNNNNNTGG 1 cut(s) 281
BstZ17I GTATAC 1 cut(s) 1540
BsuRI GGCC 2 cut(s) 97, 182
BtrI CACGTC 1 cut(s) 647
BtsCI GGATG 2 cut(s) 535, 829
BtsI GCAGTG 1 cut(s) 1423
BtsIMutI CAGTG 4 cut(s) 395, 703, 816, 1423
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 6 cut(s) 78, 184, 231, 287, 1342, 1414
CaiI CAGNNNCTG 1 cut(s) 59
Cfr13I GGNCC 7 cut(s) 180, 812, 1118, 1139, 1526, 1652, 1661
Csp6I GTAC 3 cut(s) 684, 892, 1291
CviAII CATG 8 cut(s) 259, 356, 441, 567, 1052, 1175, 1337, 1352
CviQI GTAC 3 cut(s) 684, 892, 1291
DdeI CTNAG 6 cut(s) 153, 756, 1157, 1464, 1491, 1554
DpnI GATC 5 cut(s) 158, 465, 783, 999, 1568
DpnII GATC 5 cut(s) 156, 463, 781, 997, 1566
Eam1104I CTCTTC 2 cut(s) 378, 831
EarI CTCTTC 2 cut(s) 378, 831
Eco24I GRGCYC 1 cut(s) 410
Eco31I GGTCTC 2 cut(s) 634, 679
Eco32I GATATC 2 cut(s) 1150, 1598
Eco47I GGWCC 6 cut(s) 812, 1118, 1139, 1526, 1652, 1661
Eco57I CTGAAG 1 cut(s) 1352
Eco91I GGTNACC 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 1652
EcoO65I GGTNACC 1 cut(s) 456
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 5 cut(s) 100, 511, 863, 946, 1495
EcoRV GATATC 2 cut(s) 1150, 1598
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 1 cut(s) 410
FaeI CATG 8 cut(s) 262, 359, 444, 570, 1055, 1178, 1340, 1355
FalI AAGNNNNNCTT 2 cut(s) 604, 636
FaqI GGGAC 5 cut(s) 679, 970, 1087, 1092, 1372
FatI CATG 8 cut(s) 258, 355, 440, 566, 1051, 1174, 1336, 1351
FauI CCCGC 1 cut(s) 1292
FauNDI CATATG 1 cut(s) 1279
FbaI TGATCA 1 cut(s) 781
FblI GTMKAC 2 cut(s) 843, 1539
Fnu4HI GCNGC 5 cut(s) 369, 1410, 1441, 1461, 1505
FokI GGATG 2 cut(s) 542, 816
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 5 cut(s) 369, 1410, 1441, 1461, 1505
FspBI CTAG 1 cut(s) 990
GluI GCNGC 5 cut(s) 369, 1410, 1441, 1461, 1505
GsaI CCCAGC 1 cut(s) 1359
GsuI CTGGAG 3 cut(s) 84, 727, 1242
HaeIII GGCC 2 cut(s) 97, 182
Hin1II CATG 8 cut(s) 262, 359, 444, 570, 1055, 1178, 1340, 1355
HindIII AAGCTT 2 cut(s) 1342, 1382
HinfI GANTC 6 cut(s) 28, 174, 249, 745, 751, 1433
HphI GGTGA 3 cut(s) 116, 151, 1007
Hpy166II GTNNAC 2 cut(s) 844, 1540
Hpy188I TCNGA 3 cut(s) 750, 1139, 1205
Hpy188III TCNNGA 8 cut(s) 120, 242, 317, 350, 377, 765, 779, 833
Hpy8I GTNNAC 2 cut(s) 844, 1540
Hpy99I CGWCG 1 cut(s) 1327
HpyAV CCTTC 5 cut(s) 76, 101, 467, 1376, 1643
HpyCH4III ACNGT 2 cut(s) 214, 820
HpyCH4IV ACGT 2 cut(s) 646, 1047
HpyF10VI GCNNNNNNNGC 1 cut(s) 1501
HpyF3I CTNAG 6 cut(s) 153, 756, 1157, 1464, 1491, 1554
HpySE526I ACGT 2 cut(s) 646, 1047
Hsp92II CATG 8 cut(s) 262, 359, 444, 570, 1055, 1178, 1340, 1355
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 5 cut(s) 156, 463, 781, 997, 1566
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 5 cut(s) 85, 352, 405, 881, 1189
Lsp1109I GCAGC 2 cut(s) 380, 1491
LweI GCATC 3 cut(s) 609, 1264, 1396
MaeI CTAG 1 cut(s) 990
MaeII ACGT 2 cut(s) 646, 1047
MaeIII GTNAC 8 cut(s) 214, 456, 476, 499, 701, 899, 1181, 1486
MalI GATC 5 cut(s) 158, 465, 783, 999, 1568
MbiI CCGCTC 2 cut(s) 884, 1412
MboI GATC 5 cut(s) 156, 463, 781, 997, 1566
MboII GAAGA 8 cut(s) 365, 608, 754, 846, 848, 862, 1014, 1684
MfeI CAATTG 2 cut(s) 921, 1678
MhlI GDGCHC 1 cut(s) 410
MmeI TCCRAC 2 cut(s) 507, 1117
MnlI CCTC 9 cut(s) 99, 108, 139, 190, 418, 712, 832, 1255, 1639
Mph1103I ATGCAT 1 cut(s) 1279
MseI TTAA 2 cut(s) 591, 1113
MslI CAYNNNNRTG 6 cut(s) 300, 398, 400, 1091, 1179, 1257
MspA1I CMGCKG 2 cut(s) 341, 1257
MspR9I CCNGG 5 cut(s) 102, 513, 865, 948, 1497
MunI CAATTG 2 cut(s) 921, 1678
Mva1269I GAATGC 1 cut(s) 774
MvaI CCWGG 5 cut(s) 102, 513, 865, 948, 1497
MwoI GCNNNNNNNGC 1 cut(s) 1501
NdeI CATATG 1 cut(s) 1279
NdeII GATC 5 cut(s) 156, 463, 781, 997, 1566
NlaIII CATG 8 cut(s) 262, 359, 444, 570, 1055, 1178, 1340, 1355
NlaIV GGNNCC 3 cut(s) 407, 814, 1527
NmuCI GTSAC 3 cut(s) 701, 899, 1181
NsiI ATGCAT 1 cut(s) 1279
NspI RCATGY 5 cut(s) 359, 570, 1178, 1340, 1355
OliI CACNNNNGTG 3 cut(s) 398, 400, 1257
PciSI GCTCTTC 1 cut(s) 378
PctI GAATGC 1 cut(s) 774
PfeI GAWTC 6 cut(s) 28, 174, 249, 745, 751, 1433
PflFI GACNNNGTC 1 cut(s) 645
PflMI CCANNNNNTGG 3 cut(s) 259, 1067, 1355
PkrI GCNGC 5 cut(s) 370, 1411, 1442, 1462, 1506
PpuMI RGGWCCY 1 cut(s) 1652
PshAI GACNNNNGTC 1 cut(s) 671
PsiI TTATAA 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 1652
Psp6I CCWGG 5 cut(s) 100, 511, 863, 946, 1495
PspEI GGTNACC 1 cut(s) 456
PspFI CCCAGC 1 cut(s) 1355
PspGI CCWGG 5 cut(s) 100, 511, 863, 946, 1495
PspN4I GGNNCC 3 cut(s) 407, 814, 1527
PspPI GGNCC 7 cut(s) 180, 812, 1118, 1139, 1526, 1652, 1661
PspPPI RGGWCCY 1 cut(s) 1652
PstI CTGCAG 3 cut(s) 698, 729, 1509
PstNI CAGNNNCTG 1 cut(s) 59
PsyI GACNNNGTC 1 cut(s) 645
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 3 cut(s) 685, 893, 1292
RsaNI GTAC 3 cut(s) 684, 892, 1291
RseI CAYNNNNRTG 6 cut(s) 300, 398, 400, 1091, 1179, 1257
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 2 cut(s) 591, 1113
SatI GCNGC 5 cut(s) 369, 1410, 1441, 1461, 1505
Sau3AI GATC 5 cut(s) 156, 463, 781, 997, 1566
Sau96I GGNCC 7 cut(s) 180, 812, 1118, 1139, 1526, 1652, 1661
ScrFI CCNGG 5 cut(s) 102, 513, 865, 948, 1497
SduI GDGCHC 1 cut(s) 410
SfaNI GCATC 3 cut(s) 609, 1264, 1396
SfcI CTRYAG 5 cut(s) 678, 694, 725, 1505, 1656
SinI GGWCC 6 cut(s) 812, 1118, 1139, 1526, 1652, 1661
SmiMI CAYNNNNRTG 6 cut(s) 300, 398, 400, 1091, 1179, 1257
SsiI CCGC 6 cut(s) 884, 1257, 1299, 1410, 1441, 1460
SspMI CTAG 1 cut(s) 990
StyD4I CCNGG 5 cut(s) 100, 511, 863, 946, 1495
TaaI ACNGT 2 cut(s) 214, 820
TaiI ACGT 2 cut(s) 649, 1050
TaqI TCGA 4 cut(s) 316, 376, 1000, 1322
TatI WGTACW 2 cut(s) 891, 1290
TauI GCSGC 3 cut(s) 1412, 1443, 1463
TfiI GAWTC 6 cut(s) 28, 174, 249, 745, 751, 1433
Tru1I TTAA 2 cut(s) 591, 1113
Tru9I TTAA 2 cut(s) 591, 1113
TscAI CASTG 4 cut(s) 402, 710, 823, 1423
TseFI GTSAC 3 cut(s) 701, 899, 1181
TseI GCWGC 2 cut(s) 368, 1504
Tsp45I GTSAC 3 cut(s) 701, 899, 1181
TspDTI ATGAA 7 cut(s) 429, 546, 779, 818, 942, 1040, 1464
TspRI CASTG 4 cut(s) 402, 710, 823, 1423
Tth111I GACNNNGTC 1 cut(s) 645
Van91I CCANNNNNTGG 3 cut(s) 259, 1067, 1355
VpaK11BI GGWCC 6 cut(s) 812, 1118, 1139, 1526, 1652, 1661
XapI RAATTY 4 cut(s) 237, 786, 940, 1623
XceI RCATGY 5 cut(s) 359, 570, 1178, 1340, 1355
XcmI CCANNNNNNNNNTGG 1 cut(s) 90
XmiI GTMKAC 2 cut(s) 843, 1539
XspI CTAG 1 cut(s) 990
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.