MD10G1176600.v1.1

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007670
Physical Location & Seq
Reverse (-)
26879966 .. 26883670
3705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1176600.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGTTCGGGTGCAAGTGCTTCCATTGGAATCAACTCACCGATTTATTCCCACCTGAGCCTGAAGAGCCCTTCTCTCTGCCTGATCCCATCCCTCAATGGCCTTCAGGTGAAGGTTTTGCTTCTGGAAGAATCAATCTCGGAGAAATAGAGGTTTTCAAGATAACCAGATTTGAGTTTATCTGGGCCTGCACTCCATCAGAGGACAAGCAAAAAACTGTTACGTTTTATAAGCCGGTGGGAATACCTGATGGATTCCATAGCATTGGTCACTATTGCCAATCTAAGGAGAAGCCTTTACATGGTTTTGTTCTTGTGGTTCAAGAGGCAGAGTTGCCAGAAACAGCTGATGTTGGGGAGCCTGTCAAGTTGCCAGCTCTTCGAGAGCCGCTTGATTACAAGTTAGTATGGAGCCCTGATGATGGGAATGAGGACAATTATGGTGCATATGGTTACTTTTGGCATCCTCAGCCACCAGAAGGTTACAAAGCAATGGGCTTTTTAGTGACTAGCAGGCCTGATAAACCTAGGTTGGATGAAGTGAGATGTGTTCGAGTTGATTTGACTGATGTTTGTGAAACTTACCGCCCAATTCTTAATGCCATTACAAAATCTCTAAATCTTCCTTTTCAAGTTTGGTCTATGAGACCACATCATCGGGGAACGATGGGGAAGGGGGTTTGTGCAGGTACATTTTTCTGCAGTAGCGACATGAGCATTAGAAAGGATCTGAAGCATATTGGTTGCTTGAAGAATCTGAATTCGAAGCTACCTGGAATGCCAAATCTTGATCAAATTCATTCACTCATCCATCATTATGGTCCTACCGTATTCTTTCATCCAGATGAGATTTACTTGCCATCTTCTGTTTTATGGTTCTTCAAAAGTGGAGCACTACTGTTCAAGACTGGCACGTCAGTTGGTGAGCCTATTGATGGCAGTGGCTCAAATTTGCCTGGCGGTGGTTCAAATGATGGGGGGTTTTGGATAGACTTGCCAAGTGATGAACGAGGAGAGATTATAAAACGCGGAAACTTGGAAAGTGCAGAACTTTATGTTCATGTGAAACCGGCTCTTGGTGGTACTTTCACTGACATTGCAATGTGGGTGTTTTGTCCCTTCAATGGACCAGCCACTCTCAAAGTTGGGCCTATGGACTTCCCTCTTGGCAAGATTGGACAGCATGTCGGTGACTGGGAGCACTTCACACTCCGCATCTGCAACTTTTCGGGAGAGCTCTGGAGCATATACTTCTCGCAGCACAGCGGTGGCAAATGGGTGGATGCATATGATTTGGAGTACATAGATGGGAACAAAGCCATTGTCTACTCATCCAAAAATGGACACGCGAGCTACCCTCATCCGGGGACTTACCTTCAGGGGTCAGACAAGCTAGGCATTGGGATAAGGAACGATGCTGCTCGTAGTAACTTATCCGTGGATTCAAGCGTCCAGTATGAGATTGTTGCAGCCGAGTATCTTGGAGACGGGGTTGCGGCCGAGCCGTGTTGGCTGCAGTTTATGAGAGAGTGGGGTCCAACAATTGTGTACAATTCAAGAACTGAGCTTGATAAGGTGATCAATCTTTTGCCTGTGATGTTCAAGTATAATTTGGAGGACATTTTTAGTAAGTTACCGGTTGAGCTTTACGGGGAGGAAGGTCCTACCGGGCCAAAAGAGAAGAACAATTGGGTGGGAGATGAGAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

570

Amino Acids

63.68

Weight (kDa)

5.32

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 21 - 567 3.5e-265 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 228, 1019
AasI GACNNNNNNGTC 1 cut(s) 910
Acc36I ACCTGC 1 cut(s) 674
AccI GTMKAC 1 cut(s) 1323
AccII CGCG 2 cut(s) 1026, 1346
AciI CCGC 7 cut(s) 386, 583, 957, 1026, 1210, 1263, 1493
AclWI GGATC 2 cut(s) 77, 732
AcoI YGGCCR 1 cut(s) 1494
AcsI RAATTY 3 cut(s) 757, 792, 946
AcuI CTGAAG 4 cut(s) 81, 87, 749, 1358
AfaI GTAC 4 cut(s) 688, 1081, 1298, 1547
AgeI ACCGGT 1 cut(s) 1633
AhdI GACNNNNNGTC 1 cut(s) 1181
AjiI CACGTC 1 cut(s) 912
AjnI CCWGG 2 cut(s) 769, 952
AleI CACNNNNGTG 1 cut(s) 1263
AluBI AGCT 9 cut(s) 344, 374, 766, 1234, 1350, 1390, 1564, 1642, 1707
AluI AGCT 9 cut(s) 344, 374, 766, 1234, 1350, 1390, 1564, 1642, 1707
Alw21I GWGCWC 3 cut(s) 892, 1200, 1236
Alw26I GTCTC 2 cut(s) 637, 1476
AlwI GGATC 2 cut(s) 77, 732
AoxI GGCC 6 cut(s) 98, 183, 512, 1145, 1494, 1667
ApeKI GCWGC 4 cut(s) 1255, 1415, 1466, 1510
ApoI RAATTY 3 cut(s) 757, 792, 946
ArsI GACNNNNNNTTYG 2 cut(s) 1305, 1337
AsiGI ACCGGT 1 cut(s) 1633
AspA2I CCTAGG 1 cut(s) 524
AspS9I GGNCC 7 cut(s) 183, 818, 1124, 1145, 1532, 1658, 1667
AsuC2I CCSGG 2 cut(s) 1362, 1666
AsuHPI GGTGA 5 cut(s) 28, 119, 932, 1199, 1585
AsuII TTCGAA 1 cut(s) 761
AvaII GGWCC 4 cut(s) 818, 1124, 1532, 1658
AvrII CCTAGG 1 cut(s) 524
BanII GRGCYC 3 cut(s) 69, 413, 1236
Bbv12I GWGCWC 3 cut(s) 892, 1200, 1236
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 4 cut(s) 1267, 1402, 1478, 1497
BceAI ACGGC 1 cut(s) 1486
BciT130I CCWGG 2 cut(s) 771, 954
BclI TGATCA 2 cut(s) 787, 1575
BcnI CCSGG 2 cut(s) 1362, 1666
BcoDI GTCTC 2 cut(s) 637, 1476
BfaI CTAG 4 cut(s) 507, 525, 1391, 1708
BfmI CTRYAG 2 cut(s) 697, 1511
BfuAI ACCTGC 1 cut(s) 674
BglI GCCNNNNNGGC 1 cut(s) 1507
BisI GCNGC 6 cut(s) 386, 1256, 1416, 1467, 1494, 1511
BlnI CCTAGG 1 cut(s) 524
BlsI GCNGC 6 cut(s) 387, 1257, 1417, 1468, 1495, 1512
Bme1390I CCNGG 4 cut(s) 771, 954, 1362, 1666
Bme18I GGWCC 4 cut(s) 818, 1124, 1532, 1658
BmeRI GACNNNNNGTC 1 cut(s) 1181
BmgBI CACGTC 1 cut(s) 912
BmgT120I GGNCC 7 cut(s) 183, 818, 1124, 1145, 1532, 1658, 1667
BmiI GGNNCC 3 cut(s) 357, 410, 1533
BmrFI CCNGG 4 cut(s) 771, 954, 1362, 1666
BmrI ACTGGG 1 cut(s) 1201
BmsI GCATC 4 cut(s) 469, 1221, 1270, 1402
BmuI ACTGGG 1 cut(s) 1201
BplI GAGNNNNNCTC 4 cut(s) 56, 88, 1339, 1371
BpmI CTGGAG 1 cut(s) 1258
Bpu10I CCTNAGC 2 cut(s) 54, 465
Bpu14I TTCGAA 1 cut(s) 761
BpuMI CCSGG 2 cut(s) 1362, 1666
BsaI GGTCTC 1 cut(s) 637
BsaJI CCNNGG 3 cut(s) 524, 1361, 1434
BsaWI WCCGGW 1 cut(s) 1633
BsaXI ACNNNNNCTCC 2 cut(s) 1473, 1503
Bse118I RCCGGY 3 cut(s) 232, 1066, 1633
Bse1I ACTGG 3 cut(s) 910, 1196, 1450
Bse3DI GCAATG 3 cut(s) 495, 1092, 1104
BseBI CCWGG 2 cut(s) 771, 954
BseDI CCNNGG 3 cut(s) 524, 1361, 1434
BseGI GGATG 8 cut(s) 87, 460, 538, 804, 835, 1285, 1328, 1357
BseMI GCAATG 3 cut(s) 495, 1092, 1104
BseMII CTCAG 3 cut(s) 45, 479, 1551
BseNI ACTGG 3 cut(s) 910, 1196, 1450
BseRI GAGGAG 1 cut(s) 1023
BseX3I CGGCCG 1 cut(s) 1494
BseXI GCAGC 4 cut(s) 1267, 1402, 1478, 1497
BsgI GTGCAG 3 cut(s) 172, 702, 1062
Bsh1236I CGCG 2 cut(s) 1026, 1346
Bsh1285I CGRYCG 1 cut(s) 1497
BshFI GGCC 6 cut(s) 100, 185, 514, 1147, 1496, 1669
BshTI ACCGGT 1 cut(s) 1633
BsiEI CGRYCG 1 cut(s) 1497
BsiHKAI GWGCWC 3 cut(s) 892, 1200, 1236
BsiSI CCGG 5 cut(s) 233, 1067, 1361, 1634, 1665
BslFI GGGAC 2 cut(s) 1098, 1378
BsmAI GTCTC 2 cut(s) 637, 1476
BsmBI CGTCTC 1 cut(s) 1476
BsmFI GGGAC 2 cut(s) 1098, 1378
BsmI GAATGC 1 cut(s) 780
BsnI GGCC 6 cut(s) 100, 185, 514, 1147, 1496, 1669
Bso31I GGTCTC 1 cut(s) 637
Bsp119I TTCGAA 1 cut(s) 761
Bsp1286I GDGCHC 5 cut(s) 69, 413, 892, 1200, 1236
Bsp1407I TGTACA 1 cut(s) 1545
Bsp143I GATC 4 cut(s) 82, 724, 787, 1575
BspACI CCGC 7 cut(s) 386, 583, 957, 1026, 1210, 1263, 1493
BspANI GGCC 6 cut(s) 100, 185, 514, 1147, 1496, 1669
BspCNI CTCAG 3 cut(s) 46, 478, 1552
BspFNI CGCG 2 cut(s) 1026, 1346
BspLI GGNNCC 3 cut(s) 357, 410, 1533
BspMAI CTGCAG 2 cut(s) 701, 1515
BspMI ACCTGC 1 cut(s) 674
BspPI GGATC 2 cut(s) 77, 732
BspQI GCTCTTC 2 cut(s) 57, 381
BspT104I TTCGAA 1 cut(s) 761
BspTNI GGTCTC 1 cut(s) 637
BsrDI GCAATG 3 cut(s) 495, 1092, 1104
BsrFI RCCGGY 3 cut(s) 232, 1066, 1633
BsrGI TGTACA 1 cut(s) 1545
BsrI ACTGG 3 cut(s) 910, 1196, 1450
BssAI RCCGGY 3 cut(s) 232, 1066, 1633
BssECI CCNNGG 3 cut(s) 524, 1361, 1434
BssMI GATC 4 cut(s) 82, 724, 787, 1575
BssT1I CCWWGG 1 cut(s) 524
Bst2UI CCWGG 2 cut(s) 771, 954
Bst4CI ACNGT 3 cut(s) 217, 826, 897
Bst6I CTCTTC 2 cut(s) 57, 381
BstAUI TGTACA 1 cut(s) 1545
BstBI TTCGAA 1 cut(s) 761
BstC8I GCNNGC 4 cut(s) 187, 372, 512, 1348
BstDEI CTNAG 4 cut(s) 54, 282, 465, 1560
BstDSI CCRYGG 1 cut(s) 1434
BstF5I GGATG 8 cut(s) 87, 460, 538, 804, 835, 1285, 1328, 1357
BstFNI CGCG 2 cut(s) 1026, 1346
BstKTI GATC 4 cut(s) 85, 727, 790, 1578
BstMAI GTCTC 2 cut(s) 637, 1476
BstMBI GATC 4 cut(s) 82, 724, 787, 1575
BstMCI CGRYCG 1 cut(s) 1497
BstMWI GCNNNNNNNGC 4 cut(s) 64, 466, 711, 1507
BstNI CCWGG 2 cut(s) 771, 954
BstNSI RCATGY 1 cut(s) 1184
BstSCI CCNGG 4 cut(s) 769, 952, 1360, 1664
BstSFI CTRYAG 2 cut(s) 697, 1511
BstUI CGCG 2 cut(s) 1026, 1346
BstV1I GCAGC 4 cut(s) 1267, 1402, 1478, 1497
BstX2I RGATCY 1 cut(s) 724
BstXI CCANNNNNNTGG 2 cut(s) 263, 815
BstYI RGATCY 1 cut(s) 724
BstZI CGGCCG 1 cut(s) 1494
BsuRI GGCC 6 cut(s) 100, 185, 514, 1147, 1496, 1669
BtgI CCRYGG 1 cut(s) 1434
BtrI CACGTC 1 cut(s) 912
BtsCI GGATG 8 cut(s) 87, 460, 538, 804, 835, 1285, 1328, 1357
BtsI GCAGTG 1 cut(s) 943
BtsIMutI CAGTG 2 cut(s) 943, 1086
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 4 cut(s) 187, 372, 512, 1348
Cfr10I RCCGGY 3 cut(s) 232, 1066, 1633
Cfr13I GGNCC 7 cut(s) 183, 818, 1124, 1145, 1532, 1658, 1667
CseI GACGC 1 cut(s) 1435
Csp6I GTAC 4 cut(s) 687, 1080, 1297, 1546
CspAI ACCGGT 1 cut(s) 1633
CviAII CATG 4 cut(s) 299, 709, 1058, 1181
CviQI GTAC 4 cut(s) 687, 1080, 1297, 1546
DdeI CTNAG 4 cut(s) 54, 282, 465, 1560
DpnI GATC 4 cut(s) 84, 726, 789, 1577
DpnII GATC 4 cut(s) 82, 724, 787, 1575
DrdI GACNNNNNNGTC 1 cut(s) 910
DriI GACNNNNNGTC 1 cut(s) 1181
DseDI GACNNNNNNGTC 1 cut(s) 910
EaeI YGGCCR 1 cut(s) 1494
EagI CGGCCG 1 cut(s) 1494
Eam1104I CTCTTC 2 cut(s) 57, 381
Eam1105I GACNNNNNGTC 1 cut(s) 1181
EarI CTCTTC 2 cut(s) 57, 381
Ecl136II GAGCTC 1 cut(s) 1234
EclXI CGGCCG 1 cut(s) 1494
Eco130I CCWWGG 1 cut(s) 524
Eco147I AGGCCT 1 cut(s) 514
Eco24I GRGCYC 3 cut(s) 69, 413, 1236
Eco31I GGTCTC 1 cut(s) 637
Eco47I GGWCC 4 cut(s) 818, 1124, 1532, 1658
Eco52I CGGCCG 1 cut(s) 1494
Eco53kI GAGCTC 1 cut(s) 1234
Eco57I CTGAAG 4 cut(s) 81, 87, 749, 1358
EcoICRI GAGCTC 1 cut(s) 1234
EcoO109I RGGNCCY 1 cut(s) 1658
EcoRI GAATTC 1 cut(s) 757
EcoRII CCWGG 2 cut(s) 769, 952
EcoT14I CCWWGG 1 cut(s) 524
EcoT22I ATGCAT 1 cut(s) 1285
EcoT38I GRGCYC 3 cut(s) 69, 413, 1236
ErhI CCWWGG 1 cut(s) 524
Esp3I CGTCTC 1 cut(s) 1476
FaeI CATG 4 cut(s) 302, 712, 1061, 1184
FaqI GGGAC 2 cut(s) 1098, 1378
FatI CATG 4 cut(s) 298, 708, 1057, 1180
FauNDI CATATG 2 cut(s) 445, 1285
FbaI TGATCA 2 cut(s) 787, 1575
FblI GTMKAC 1 cut(s) 1323
Fnu4HI GCNGC 6 cut(s) 386, 1256, 1416, 1467, 1494, 1511
FokI GGATG 8 cut(s) 74, 447, 545, 791, 822, 1292, 1315, 1344
FriOI GRGCYC 3 cut(s) 69, 413, 1236
Fsp4HI GCNGC 6 cut(s) 386, 1256, 1416, 1467, 1494, 1511
FspBI CTAG 4 cut(s) 507, 525, 1391, 1708
GluI GCNGC 6 cut(s) 386, 1256, 1416, 1467, 1494, 1511
GsuI CTGGAG 1 cut(s) 1258
HaeIII GGCC 6 cut(s) 100, 185, 514, 1147, 1496, 1669
HapII CCGG 5 cut(s) 233, 1067, 1361, 1634, 1665
HgaI GACGC 1 cut(s) 1435
Hin1II CATG 4 cut(s) 302, 712, 1061, 1184
HinfI GANTC 5 cut(s) 28, 129, 252, 751, 1439
HpaII CCGG 5 cut(s) 233, 1067, 1361, 1634, 1665
HphI GGTGA 5 cut(s) 28, 119, 932, 1199, 1585
Hpy166II GTNNAC 2 cut(s) 1324, 1546
Hpy188I TCNGA 5 cut(s) 140, 199, 729, 756, 1384
Hpy8I GTNNAC 2 cut(s) 1324, 1546
HpyAV CCTTC 8 cut(s) 79, 104, 111, 470, 664, 1126, 1382, 1649
HpyCH4III ACNGT 3 cut(s) 217, 826, 897
HpyCH4IV ACGT 2 cut(s) 221, 911
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 466, 711, 1507
HpyF3I CTNAG 4 cut(s) 54, 282, 465, 1560
HpySE526I ACGT 2 cut(s) 221, 911
Hsp92II CATG 4 cut(s) 302, 712, 1061, 1184
Ksp22I TGATCA 2 cut(s) 787, 1575
Kzo9I GATC 4 cut(s) 82, 724, 787, 1575
LguI GCTCTTC 2 cut(s) 57, 381
LmnI GCTCC 5 cut(s) 355, 408, 887, 1195, 1239
Lsp1109I GCAGC 4 cut(s) 1267, 1402, 1478, 1497
LweI GCATC 4 cut(s) 469, 1221, 1270, 1402
MaeI CTAG 4 cut(s) 507, 525, 1391, 1708
MaeII ACGT 2 cut(s) 221, 911
MaeIII GTNAC 8 cut(s) 217, 266, 449, 479, 502, 1187, 1424, 1629
MalI GATC 4 cut(s) 84, 726, 789, 1577
MboI GATC 4 cut(s) 82, 724, 787, 1575
MboII GAAGA 8 cut(s) 74, 138, 368, 611, 760, 852, 868, 1690
MfeI CAATTG 2 cut(s) 1539, 1684
MflI RGATCY 1 cut(s) 724
MhlI GDGCHC 5 cut(s) 69, 413, 892, 1200, 1236
MluCI AATT 9 cut(s) 433, 588, 757, 792, 946, 1539, 1549, 1606, 1684
MmeI TCCRAC 2 cut(s) 510, 1559
Mph1103I ATGCAT 1 cut(s) 1285
MseI TTAA 1 cut(s) 594
MslI CAYNNNNRTG 5 cut(s) 813, 840, 1097, 1185, 1263
MspA1I CMGCKG 2 cut(s) 344, 1263
MspI CCGG 5 cut(s) 233, 1067, 1361, 1634, 1665
MspR9I CCNGG 4 cut(s) 771, 954, 1362, 1666
MunI CAATTG 2 cut(s) 1539, 1684
Mva1269I GAATGC 1 cut(s) 780
MvaI CCWGG 2 cut(s) 771, 954
MvnI CGCG 2 cut(s) 1026, 1346
MwoI GCNNNNNNNGC 4 cut(s) 64, 466, 711, 1507
NciI CCSGG 2 cut(s) 1362, 1666
NdeI CATATG 2 cut(s) 445, 1285
NdeII GATC 4 cut(s) 82, 724, 787, 1575
NlaIII CATG 4 cut(s) 302, 712, 1061, 1184
NlaIV GGNNCC 3 cut(s) 357, 410, 1533
NmeAIII GCCGAG 2 cut(s) 1495, 1522
NmuCI GTSAC 3 cut(s) 266, 502, 1187
NsiI ATGCAT 1 cut(s) 1285
NspI RCATGY 1 cut(s) 1184
NspV TTCGAA 1 cut(s) 761
OliI CACNNNNGTG 1 cut(s) 1263
PceI AGGCCT 1 cut(s) 514
PciSI GCTCTTC 2 cut(s) 57, 381
PctI GAATGC 1 cut(s) 780
PfeI GAWTC 5 cut(s) 28, 129, 252, 751, 1439
PinAI ACCGGT 1 cut(s) 1633
PkrI GCNGC 6 cut(s) 387, 1257, 1417, 1468, 1495, 1512
PpuMI RGGWCCY 1 cut(s) 1658
PsiI TTATAA 2 cut(s) 228, 1019
Psp124BI GAGCTC 1 cut(s) 1236
Psp5II RGGWCCY 1 cut(s) 1658
Psp6I CCWGG 2 cut(s) 769, 952
PspGI CCWGG 2 cut(s) 769, 952
PspN4I GGNNCC 3 cut(s) 357, 410, 1533
PspPI GGNCC 7 cut(s) 183, 818, 1124, 1145, 1532, 1658, 1667
PspPPI RGGWCCY 1 cut(s) 1658
PstI CTGCAG 2 cut(s) 701, 1515
PsuI RGATCY 1 cut(s) 724
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 4 cut(s) 688, 1081, 1298, 1547
RsaNI GTAC 4 cut(s) 687, 1080, 1297, 1546
RseI CAYNNNNRTG 5 cut(s) 813, 840, 1097, 1185, 1263
SacI GAGCTC 1 cut(s) 1236
SapI GCTCTTC 2 cut(s) 57, 381
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 6 cut(s) 386, 1256, 1416, 1467, 1494, 1511
Sau3AI GATC 4 cut(s) 82, 724, 787, 1575
Sau96I GGNCC 7 cut(s) 183, 818, 1124, 1145, 1532, 1658, 1667
ScrFI CCNGG 4 cut(s) 771, 954, 1362, 1666
SduI GDGCHC 5 cut(s) 69, 413, 892, 1200, 1236
SfaNI GCATC 4 cut(s) 469, 1221, 1270, 1402
SfcI CTRYAG 2 cut(s) 697, 1511
SfuI TTCGAA 1 cut(s) 761
SinI GGWCC 4 cut(s) 818, 1124, 1532, 1658
SmiMI CAYNNNNRTG 5 cut(s) 813, 840, 1097, 1185, 1263
Sse9I AATT 9 cut(s) 433, 588, 757, 792, 946, 1539, 1549, 1606, 1684
SseBI AGGCCT 1 cut(s) 514
SsiI CCGC 7 cut(s) 386, 583, 957, 1026, 1210, 1263, 1493
SspMI CTAG 4 cut(s) 507, 525, 1391, 1708
SstI GAGCTC 1 cut(s) 1236
StuI AGGCCT 1 cut(s) 514
StyD4I CCNGG 4 cut(s) 769, 952, 1360, 1664
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 3 cut(s) 217, 826, 897
TaiI ACGT 2 cut(s) 224, 914
TaqI TCGA 3 cut(s) 379, 550, 761
TasI AATT 9 cut(s) 433, 588, 757, 792, 946, 1539, 1549, 1606, 1684
TatI WGTACW 2 cut(s) 1296, 1545
TauI GCSGC 2 cut(s) 388, 1496
TfiI GAWTC 5 cut(s) 28, 129, 252, 751, 1439
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 2 cut(s) 943, 1093
TseFI GTSAC 3 cut(s) 266, 502, 1187
TseI GCWGC 4 cut(s) 1255, 1415, 1466, 1510
Tsp45I GTSAC 3 cut(s) 266, 502, 1187
TspDTI ATGAA 5 cut(s) 549, 785, 824, 1017, 1046
TspGWI ACGGA 1 cut(s) 1423
TspRI CASTG 2 cut(s) 943, 1093
VpaK11BI GGWCC 4 cut(s) 818, 1124, 1532, 1658
XapI RAATTY 3 cut(s) 757, 792, 946
XceI RCATGY 1 cut(s) 1184
XmaJI CCTAGG 1 cut(s) 524
XmiI GTMKAC 1 cut(s) 1323
XspI CTAG 4 cut(s) 507, 525, 1391, 1708
Zsp2I ATGCAT 1 cut(s) 1285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.