Rroxscaffold_7G00182960

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007670
Physical Location & Seq
Reverse (-)
22033930 .. 22037791
3862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00182960.1

Sequence Viewer

Length: 714 bp
ATGGGTCATTATTGCCAACTCAATGGTAAGCCTTTACAGGTTTATGTTCTTGTGGTTCGGGAAGTAGATTTGTCAGAAACAGCTGGTGTTCAGGAGCATGTGAAGTCAGCAGCTCTTCGAGAGCCACTTGATTACACACTGGTGTGGAGCACTGATGATGGGAATGAGGAAAGTTCTGGTTCATGTGGCTACTTTTGGTTACCTGATCCACCTGAAGGTTACAGAGCAATGGGCTTTTTAGTTACCAACAAGCCAGGAAAACCTGAGTTGGATGAAGTAAGGTGTGTTAGAGCTGATTTGAATGACACATGCGAAACTCACATCCTACTTTTTAATACCAGCATCAAATCTGCAAGTCTTCCTTTTCAAGTTTGGACTATGAGACCACGTCATCGGGGAATGATGGGGACAGGGGTCTCTGTAGGTACATTTTTCTGCAGTAGTCACTGGAGTACTGGAGAGGAACTGCAGATTGGTTGCTTAAAGAATCTGAATCCTAAGCTCTCTGGAATGCCAAATCTTGATCAAATTCATTCACTTATCAATCATTCTGGACCCACTGTTTTCTTTCATCAAGAAGAGGTCTACTTGCCATCTTCTGTTGCCTGGTTCTTCAAAAATGGAGCGCTATTGTACAAAAGCGGCACAGATGTGGGCGAAGCAATTGATGAAAGTGGCTCAAATTTGCCTGGTGGGGGGACAAATGATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

237

Amino Acids

26.1

Weight (kDa)

5.21

Isoelectric Point (pI)

26.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 1 - 237 7.4e-92 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 585
AciI CCGC 1 cut(s) 642
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 2 cut(s) 528, 682
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 3 cut(s) 427, 454, 635
AfeI AGCGCT 1 cut(s) 627
AfiI CCNNNNNNNGG 2 cut(s) 215, 695
AgsI TTSAA 3 cut(s) 301, 368, 616
AjiI CACGTC 1 cut(s) 389
AjnI CCWGG 3 cut(s) 253, 605, 688
AjuI GAANNNNNNNTTGG 2 cut(s) 456, 488
AleI CACNNNNGTG 3 cut(s) 140, 142, 650
AluBI AGCT 4 cut(s) 83, 113, 293, 502
AluI AGCT 4 cut(s) 83, 113, 293, 502
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 2 cut(s) 376, 421
AlwI GGATC 1 cut(s) 200
Aor51HI AGCGCT 1 cut(s) 627
ApeKI GCWGC 1 cut(s) 110
ApoI RAATTY 2 cut(s) 528, 682
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 1 cut(s) 554
AvaII GGWCC 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 1 cut(s) 122
BccI CCATC 3 cut(s) 152, 397, 601
BciT130I CCWGG 3 cut(s) 255, 607, 690
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 376, 421
BfmI CTRYAG 3 cut(s) 420, 436, 467
BfoI RGCGCY 1 cut(s) 629
BisI GCNGC 2 cut(s) 111, 643
BlsI GCNGC 2 cut(s) 112, 644
BmcAI AGTACT 1 cut(s) 454
Bme1390I CCNGG 3 cut(s) 255, 607, 690
Bme18I GGWCC 1 cut(s) 554
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 554
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 3 cut(s) 255, 607, 690
BmsI GCATC 1 cut(s) 351
BoxI GACNNNNGTC 1 cut(s) 413
BpiI GAAGAC 1 cut(s) 350
BpmI CTGGAG 2 cut(s) 469, 477
Bpu10I CCTNAGC 1 cut(s) 498
BsaI GGTCTC 2 cut(s) 376, 421
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 695
Bse1I ACTGG 3 cut(s) 144, 452, 460
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 3 cut(s) 255, 607, 690
BseGI GGATG 2 cut(s) 277, 321
BseLI CCNNNNNNNGG 2 cut(s) 215, 695
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 144, 452, 460
BseXI GCAGC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 152
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 2 cut(s) 215, 695
BsmAI GTCTC 2 cut(s) 376, 421
BsmFI GGGAC 1 cut(s) 421
BsmI GAATGC 1 cut(s) 516
Bso31I GGTCTC 2 cut(s) 376, 421
Bsp1286I GDGCHC 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 2 cut(s) 205, 523
BspACI CCGC 1 cut(s) 642
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 556
BspMAI CTGCAG 2 cut(s) 440, 471
BspPI GGATC 1 cut(s) 200
BspQI GCTCTTC 1 cut(s) 120
BspTNI GGTCTC 2 cut(s) 376, 421
BsrDI GCAATG 1 cut(s) 234
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 3 cut(s) 144, 452, 460
BssMI GATC 2 cut(s) 205, 523
Bst2UI CCWGG 3 cut(s) 255, 607, 690
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 2 cut(s) 120, 573
BstAUI TGTACA 1 cut(s) 633
BstDEI CTNAG 2 cut(s) 264, 498
BstEII GGTNACC 1 cut(s) 198
BstF5I GGATG 2 cut(s) 277, 321
BstH2I RGCGCY 1 cut(s) 629
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 2 cut(s) 208, 526
BstMAI GTCTC 2 cut(s) 376, 421
BstMBI GATC 2 cut(s) 205, 523
BstNI CCWGG 3 cut(s) 255, 607, 690
BstNSI RCATGY 2 cut(s) 101, 312
BstPAI GACNNNNGTC 1 cut(s) 413
BstPI GGTNACC 1 cut(s) 198
BstSCI CCNGG 3 cut(s) 253, 605, 688
BstSFI CTRYAG 3 cut(s) 420, 436, 467
BstV1I GCAGC 1 cut(s) 122
BstV2I GAAGAC 1 cut(s) 350
BstXI CCANNNNNNTGG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 2 cut(s) 277, 321
BtsIMutI CAGTG 4 cut(s) 137, 150, 445, 558
CfoI GCGC 1 cut(s) 628
Cfr13I GGNCC 1 cut(s) 554
Csp6I GTAC 3 cut(s) 426, 453, 634
CviAII CATG 3 cut(s) 98, 183, 309
CviQI GTAC 3 cut(s) 426, 453, 634
DdeI CTNAG 2 cut(s) 264, 498
DpnI GATC 2 cut(s) 207, 525
DpnII GATC 2 cut(s) 205, 523
Eam1104I CTCTTC 2 cut(s) 120, 573
EarI CTCTTC 2 cut(s) 120, 573
Eco31I GGTCTC 2 cut(s) 376, 421
Eco47I GGWCC 1 cut(s) 554
Eco47III AGCGCT 1 cut(s) 627
Eco57I CTGAAG 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 198
EcoO65I GGTNACC 1 cut(s) 198
EcoRII CCWGG 3 cut(s) 253, 605, 688
FaeI CATG 3 cut(s) 101, 186, 312
FaiI YATR 5 cut(s) 45, 99, 184, 310, 380
FalI AAGNNNNNCTT 2 cut(s) 346, 378
FaqI GGGAC 1 cut(s) 421
FatI CATG 3 cut(s) 97, 182, 308
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 2 cut(s) 111, 643
FokI GGATG 2 cut(s) 284, 308
Fsp4HI GCNGC 2 cut(s) 111, 643
GlaI GCGC 1 cut(s) 627
GluI GCNGC 2 cut(s) 111, 643
GsuI CTGGAG 2 cut(s) 469, 477
HaeII RGCGCY 1 cut(s) 629
HhaI GCGC 1 cut(s) 628
Hin1II CATG 3 cut(s) 101, 186, 312
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HinfI GANTC 2 cut(s) 487, 493
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 2 cut(s) 76, 492
Hpy188III TCNNGA 7 cut(s) 59, 92, 119, 507, 521, 552, 575
Hpy8I GTNNAC 1 cut(s) 586
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 3 cut(s) 353, 438, 469
HpyF3I CTNAG 2 cut(s) 264, 498
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 3 cut(s) 101, 186, 312
HspAI GCGC 1 cut(s) 626
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 2 cut(s) 205, 523
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 94, 147, 623
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 1 cut(s) 351
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 4 cut(s) 198, 218, 241, 443
MalI GATC 2 cut(s) 207, 525
MboI GATC 2 cut(s) 205, 523
MboII GAAGA 5 cut(s) 107, 350, 588, 590, 604
MfeI CAATTG 1 cut(s) 663
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 3 cut(s) 528, 663, 682
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 3 cut(s) 160, 454, 574
MseI TTAA 2 cut(s) 333, 482
MslI CAYNNNNRTG 3 cut(s) 140, 142, 650
MspA1I CMGCKG 1 cut(s) 83
MspR9I CCNGG 3 cut(s) 255, 607, 690
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 3 cut(s) 255, 607, 690
NdeII GATC 2 cut(s) 205, 523
NlaIII CATG 3 cut(s) 101, 186, 312
NlaIV GGNNCC 1 cut(s) 556
NmuCI GTSAC 1 cut(s) 443
NspI RCATGY 2 cut(s) 101, 312
OliI CACNNNNGTG 3 cut(s) 140, 142, 650
PciSI GCTCTTC 1 cut(s) 120
PctI GAATGC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 487, 493
PflFI GACNNNGTC 1 cut(s) 387
PkrI GCNGC 2 cut(s) 112, 644
PshAI GACNNNNGTC 1 cut(s) 413
Psp6I CCWGG 3 cut(s) 253, 605, 688
PspEI GGTNACC 1 cut(s) 198
PspGI CCWGG 3 cut(s) 253, 605, 688
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 1 cut(s) 554
PstI CTGCAG 2 cut(s) 440, 471
PsyI GACNNNGTC 1 cut(s) 387
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 3 cut(s) 427, 454, 635
RsaNI GTAC 3 cut(s) 426, 453, 634
RseI CAYNNNNRTG 3 cut(s) 140, 142, 650
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 333, 482
SatI GCNGC 2 cut(s) 111, 643
Sau3AI GATC 2 cut(s) 205, 523
Sau96I GGNCC 1 cut(s) 554
ScaI AGTACT 1 cut(s) 454
ScrFI CCNGG 3 cut(s) 255, 607, 690
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 1 cut(s) 351
SfcI CTRYAG 3 cut(s) 420, 436, 467
SinI GGWCC 1 cut(s) 554
SmiMI CAYNNNNRTG 3 cut(s) 140, 142, 650
Sse9I AATT 3 cut(s) 528, 663, 682
SsiI CCGC 1 cut(s) 642
StyD4I CCNGG 3 cut(s) 253, 605, 688
TaaI ACNGT 1 cut(s) 562
TaiI ACGT 1 cut(s) 391
TaqI TCGA 1 cut(s) 118
TasI AATT 3 cut(s) 528, 663, 682
TatI WGTACW 2 cut(s) 452, 633
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 2 cut(s) 487, 493
Tru1I TTAA 2 cut(s) 333, 482
Tru9I TTAA 2 cut(s) 333, 482
TscAI CASTG 4 cut(s) 144, 157, 452, 565
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 1 cut(s) 110
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 5 cut(s) 171, 288, 521, 560, 684
TspRI CASTG 4 cut(s) 144, 157, 452, 565
Tth111I GACNNNGTC 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 554
XapI RAATTY 2 cut(s) 528, 682
XceI RCATGY 2 cut(s) 101, 312
XmiI GTMKAC 1 cut(s) 585
ZrmI AGTACT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.