FvH4_2g20520

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007670
Physical Location & Seq
Reverse (-)
17207210 .. 17211289
4080 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20520.t1

Sequence Viewer

Length: 1704 bp
ATGTTTATGTGCAAGTGTTTCTATAGGAATCAACTCAATGATTTATGCCCACCGGAGCCTGAACCCTTCTCCCTGCCTGCTCCAATTCCTAAATGGCCTCCAGGTGAAGGTTTTGCTTCTGGGAAAATAATTATTGGAGAAATAGAGGTTTCCAAGATATCCAAATTTGAAAGAATCTGGACCTGCTCTCTGCCAGAGGACAAGAAAAAATGTGTTACATTTTATAAGCCAGTGGGAATTCCTGATGGATTTCGTAGCATGGGTCATTATTGCCAACTCAACGGTAAGCCTTTACACGGCTATGTTCTTGTGGTTCGAGAAGTAGAATTGCCAGAAACAGCTGTTGTTGGGGAGCATGTCAAGTCAGCAGCTCTTCGAGAGCCGCTTGATTACACATTGGTGTGGAGCCCTGATGATGGGACTGAGGAAAGTTATGGTTCATGTGGCTACTTCTGGCTACCTGATCCACCTGAAGGTTACAGAGCAATGGGCTTTTTAGTTACCAACAAGCCAGGAAAACCCGAGTTGGATGAAGTAAGGTGTGTTAGAGCTGATTTGACTGACACATGTGAACCTCACAACCTAATTTTTAATAGCCGTACCAACTCTACAAGTCTTCCTTTCCAAGTTTGGACTATGAGACCACGTCATCGTGGAATGAGGGGAACAGGGGTTTCTGTAGGTACATTTTTCTGCAGTAATCACTGGAGTAATGGAGAGGACCTGCAGATTGGGTGCTTGAAGAATCTGAATCCTAAGCTATGTGGAATGCCAAATCTTGATCAAATTCATTCACTTATCAATCATTATGGACCTACTGTCTTCTTTCATCCAGATGAGGTCTACTTGCCATCTTCAGTGGCTTGGTTCTTCAAAAATGGAGCGCTATTGTACAAAACTGGCACAGATGTAGGCGAAGCAATTGATGAAAGCGGCTCAAATTTGCCTGGTGGAAGGTCAAATGATAGGCAGTTTTGGATAGACTTACCTAGTGGTGATCAAAGAAAGAATGTTATACATGGAAACTTGGAAAGTGCAAAACTCTACGTTCATGTGAAACCAGCTCTTGGTGGGACTTTTACTGACATTGCGATGTGGGTATTTTGTCCCTTTAATGGACCAGCCACTCTCAAAGTTGGACCAATGGATATCACTCTCAGCAAGATTGGACAGCATGTGGGTGATTGGGAGCATTTCACACTTCGAATATGTAATTTTACTGGAGAGCTTTGTAGTATATACTTCTCACAGCACAGCGGTGGTCAATGGGTAGATGCATATGCTTTGGAGTACATAAGAGGGAACAAAGCTATTATCTACTCGACCAAAAATGGACATGCGAGCTTCCCGCATGCTGGGACTTACCTTCAGGGGTCTTCAAAGCTTGGGATTGGCATTAGGAATGATGCTGATCGCAGTGAGTTTTCTGTGGACTCGAGCATCCAATATGAAGTAGTTGCTGCTGAGTATCTTGGAGATGGGGTTGTTACTGAGCCAGGTTGGCTGCAGTTTATGAGAGAATGGGGACCAAAAATTGTATACAATTCAAGGACTGAGCTGGATAAGATCATCAATGTTTTGCCTGCGATGTTAAGATATTCAGTGGAGAGCATCTTTTCGAAAATTCCAGTTGAGCTTTCTGGGGAGGAAGGTCCTACAGGACCAAAGGAGAAGAACAATTGGGTGGGAGATGAAAGATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

568

Amino Acids

63.42

Weight (kDa)

5.78

Isoelectric Point (pI)

27.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 20 - 565 9.4e-266 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 225
Acc36I ACCTGC 2 cut(s) 191, 732
AccB7I CCANNNNNTGG 1 cut(s) 1067
AccI GTMKAC 2 cut(s) 843, 1539
AciI CCGC 4 cut(s) 383, 933, 1257, 1349
AclWI GGATC 1 cut(s) 458
AcsI RAATTY 5 cut(s) 164, 237, 786, 940, 1623
AcuI CTGAAG 3 cut(s) 492, 840, 1352
AfaI GTAC 4 cut(s) 601, 685, 893, 1292
AfeI AGCGCT 1 cut(s) 885
AfiI CCNNNNNNNGG 7 cut(s) 107, 296, 416, 473, 1067, 1115, 1355
AflIII ACRYGT 1 cut(s) 566
AgsI TTSAA 5 cut(s) 170, 742, 874, 1380, 1548
AhdI GACNNNNNGTC 1 cut(s) 818
AjiI CACGTC 1 cut(s) 647
AjnI CCWGG 4 cut(s) 100, 511, 946, 1495
AleI CACNNNNGTG 2 cut(s) 398, 1257
Alw26I GTCTC 1 cut(s) 634
AlwI GGATC 1 cut(s) 458
Ama87I CYCGRG 2 cut(s) 521, 1435
Aor51HI AGCGCT 1 cut(s) 885
AoxI GGCC 1 cut(s) 95
ApeKI GCWGC 3 cut(s) 368, 1460, 1504
ApoI RAATTY 5 cut(s) 164, 237, 786, 940, 1623
AspLEI GCGC 1 cut(s) 886
AspS9I GGNCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
AsuHPI GGTGA 3 cut(s) 116, 1007, 1193
AsuII TTCGAA 2 cut(s) 1204, 1619
AvaI CYCGRG 2 cut(s) 521, 1435
AvaII GGWCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
BanII GRGCYC 1 cut(s) 410
BbsI GAAGAC 3 cut(s) 608, 814, 1368
BbvI GCAGC 3 cut(s) 380, 1447, 1491
BccI CCATC 5 cut(s) 239, 410, 859, 1472, 1692
BceAI ACGGC 2 cut(s) 313, 582
BciT130I CCWGG 4 cut(s) 102, 513, 948, 1497
BclI TGATCA 2 cut(s) 781, 997
BcoDI GTCTC 1 cut(s) 634
BfaI CTAG 1 cut(s) 990
BfmI CTRYAG 6 cut(s) 22, 678, 694, 725, 1505, 1656
BfoI RGCGCY 1 cut(s) 887
BfuAI ACCTGC 2 cut(s) 191, 732
BglI GCCNNNNNGGC 1 cut(s) 1501
BisI GCNGC 5 cut(s) 369, 383, 934, 1461, 1505
BlsI GCNGC 5 cut(s) 370, 384, 935, 1462, 1506
Bme1390I CCNGG 4 cut(s) 102, 513, 948, 1497
Bme18I GGWCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
BmeRI GACNNNNNGTC 1 cut(s) 818
BmeT110I CYCGRG 2 cut(s) 521, 1435
BmgBI CACGTC 1 cut(s) 647
BmgT120I GGNCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
BmiI GGNNCC 3 cut(s) 57, 407, 1527
BmrFI CCNGG 4 cut(s) 102, 513, 948, 1497
BmsI GCATC 4 cut(s) 1264, 1396, 1449, 1620
BpiI GAAGAC 3 cut(s) 608, 814, 1368
BpmI CTGGAG 3 cut(s) 84, 727, 1242
Bpu10I CCTNAGC 1 cut(s) 756
Bpu14I TTCGAA 2 cut(s) 1204, 1619
BsaBI GATNNNNATC 1 cut(s) 1410
BsaI GGTCTC 1 cut(s) 634
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 1467, 1497
Bsc4I CCNNNNNNNGG 7 cut(s) 107, 296, 416, 473, 1067, 1115, 1355
Bse1I ACTGG 5 cut(s) 230, 710, 904, 1225, 1628
Bse3DI GCAATG 2 cut(s) 492, 1086
Bse8I GATNNNNATC 1 cut(s) 1410
BseBI CCWGG 4 cut(s) 102, 513, 948, 1497
BseGI GGATG 3 cut(s) 535, 829, 1440
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 7 cut(s) 107, 296, 416, 473, 1067, 1115, 1355
BseMI GCAATG 2 cut(s) 492, 1086
BseMII CTCAG 5 cut(s) 414, 1171, 1455, 1482, 1545
BseNI ACTGG 5 cut(s) 230, 710, 904, 1225, 1628
BseXI GCAGC 3 cut(s) 380, 1447, 1491
BseYI CCCAGC 1 cut(s) 1355
BshFI GGCC 1 cut(s) 97
BsiHKCI CYCGRG 2 cut(s) 521, 1435
BsiSI CCGG 1 cut(s) 53
BslFI GGGAC 5 cut(s) 433, 1087, 1092, 1372, 1539
BslI CCNNNNNNNGG 7 cut(s) 107, 296, 416, 473, 1067, 1115, 1355
BsmAI GTCTC 1 cut(s) 634
BsmFI GGGAC 5 cut(s) 433, 1087, 1092, 1372, 1539
BsmI GAATGC 1 cut(s) 774
BsnI GGCC 1 cut(s) 97
Bso31I GGTCTC 1 cut(s) 634
BsoBI CYCGRG 2 cut(s) 521, 1435
Bsp119I TTCGAA 2 cut(s) 1204, 1619
Bsp1286I GDGCHC 1 cut(s) 410
Bsp1407I TGTACA 1 cut(s) 891
Bsp143I GATC 5 cut(s) 463, 781, 997, 1411, 1566
BspACI CCGC 4 cut(s) 383, 933, 1257, 1349
BspANI GGCC 1 cut(s) 97
BspCNI CTCAG 5 cut(s) 415, 1170, 1456, 1483, 1546
BspLI GGNNCC 3 cut(s) 57, 407, 1527
BspMAI CTGCAG 3 cut(s) 698, 729, 1509
BspMI ACCTGC 2 cut(s) 191, 732
BspPI GGATC 1 cut(s) 458
BspQI GCTCTTC 1 cut(s) 378
BspT104I TTCGAA 2 cut(s) 1204, 1619
BspTNI GGTCTC 1 cut(s) 634
BsrDI GCAATG 2 cut(s) 492, 1086
BsrGI TGTACA 1 cut(s) 891
BsrI ACTGG 5 cut(s) 230, 710, 904, 1225, 1628
BssMI GATC 5 cut(s) 463, 781, 997, 1411, 1566
BssNAI GTATAC 1 cut(s) 1540
Bst1107I GTATAC 1 cut(s) 1540
Bst2UI CCWGG 4 cut(s) 102, 513, 948, 1497
Bst4CI ACNGT 2 cut(s) 284, 820
Bst6I CTCTTC 1 cut(s) 378
BstAUI TGTACA 1 cut(s) 891
BstBI TTCGAA 2 cut(s) 1204, 1619
BstC8I GCNNGC 4 cut(s) 78, 1342, 1353, 1584
BstDEI CTNAG 6 cut(s) 423, 756, 1157, 1464, 1491, 1554
BstF5I GGATG 3 cut(s) 535, 829, 1440
BstH2I RGCGCY 1 cut(s) 887
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 5 cut(s) 466, 784, 1000, 1414, 1569
BstMAI GTCTC 1 cut(s) 634
BstMBI GATC 5 cut(s) 463, 781, 997, 1411, 1566
BstMWI GCNNNNNNNGC 1 cut(s) 1501
BstNI CCWGG 4 cut(s) 102, 513, 948, 1497
BstNSI RCATGY 5 cut(s) 359, 570, 1178, 1340, 1355
BstSCI CCNGG 4 cut(s) 100, 511, 946, 1495
BstSFI CTRYAG 6 cut(s) 22, 678, 694, 725, 1505, 1656
BstV1I GCAGC 3 cut(s) 380, 1447, 1491
BstV2I GAAGAC 3 cut(s) 608, 814, 1368
BstZ17I GTATAC 1 cut(s) 1540
BsuRI GGCC 1 cut(s) 97
BtgZI GCGATG 2 cut(s) 1106, 1601
BtrI CACGTC 1 cut(s) 647
BtsCI GGATG 3 cut(s) 535, 829, 1440
BtsI GCAGTG 1 cut(s) 1423
BtsIMutI CAGTG 5 cut(s) 237, 703, 864, 1423, 1608
BveI ACCTGC 2 cut(s) 191, 732
Cac8I GCNNGC 4 cut(s) 78, 1342, 1353, 1584
CfoI GCGC 1 cut(s) 886
Cfr13I GGNCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
Csp6I GTAC 4 cut(s) 600, 684, 892, 1291
CviAII CATG 9 cut(s) 259, 356, 441, 567, 1019, 1052, 1175, 1337, 1352
CviQI GTAC 4 cut(s) 600, 684, 892, 1291
DdeI CTNAG 6 cut(s) 423, 756, 1157, 1464, 1491, 1554
DpnI GATC 5 cut(s) 465, 783, 999, 1413, 1568
DpnII GATC 5 cut(s) 463, 781, 997, 1411, 1566
DriI GACNNNNNGTC 1 cut(s) 818
Eam1104I CTCTTC 1 cut(s) 378
Eam1105I GACNNNNNGTC 1 cut(s) 818
EarI CTCTTC 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 634
Eco32I GATATC 2 cut(s) 159, 1150
Eco47I GGWCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
Eco47III AGCGCT 1 cut(s) 885
Eco57I CTGAAG 3 cut(s) 492, 840, 1352
Eco88I CYCGRG 2 cut(s) 521, 1435
EcoO109I RGGNCCY 2 cut(s) 721, 1652
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 4 cut(s) 100, 511, 946, 1495
EcoRV GATATC 2 cut(s) 159, 1150
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 1 cut(s) 410
FaeI CATG 9 cut(s) 262, 359, 444, 570, 1022, 1055, 1178, 1340, 1355
FalI AAGNNNNNCTT 2 cut(s) 604, 636
FaqI GGGAC 5 cut(s) 433, 1087, 1092, 1372, 1539
FatI CATG 9 cut(s) 258, 355, 440, 566, 1018, 1051, 1174, 1336, 1351
FauI CCCGC 1 cut(s) 1356
FauNDI CATATG 1 cut(s) 1279
FbaI TGATCA 2 cut(s) 781, 997
FblI GTMKAC 2 cut(s) 843, 1539
Fnu4HI GCNGC 5 cut(s) 369, 383, 934, 1461, 1505
FokI GGATG 3 cut(s) 542, 816, 1427
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 5 cut(s) 369, 383, 934, 1461, 1505
FspBI CTAG 1 cut(s) 990
GlaI GCGC 1 cut(s) 885
GluI GCNGC 5 cut(s) 369, 383, 934, 1461, 1505
GsaI CCCAGC 1 cut(s) 1359
GsuI CTGGAG 3 cut(s) 84, 727, 1242
HaeII RGCGCY 1 cut(s) 887
HaeIII GGCC 1 cut(s) 97
HapII CCGG 1 cut(s) 53
HhaI GCGC 1 cut(s) 886
Hin1II CATG 9 cut(s) 262, 359, 444, 570, 1022, 1055, 1178, 1340, 1355
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HindIII AAGCTT 1 cut(s) 1382
HinfI GANTC 5 cut(s) 28, 174, 745, 751, 1433
HpaII CCGG 1 cut(s) 53
HphI GGTGA 3 cut(s) 116, 1007, 1193
Hpy166II GTNNAC 4 cut(s) 572, 844, 1432, 1540
Hpy188I TCNGA 1 cut(s) 750
Hpy188III TCNNGA 6 cut(s) 178, 242, 317, 377, 779, 833
Hpy8I GTNNAC 4 cut(s) 572, 844, 1432, 1540
HpyAV CCTTC 6 cut(s) 76, 101, 467, 948, 1376, 1643
HpyCH4III ACNGT 2 cut(s) 284, 820
HpyCH4IV ACGT 2 cut(s) 646, 1047
HpyCH4V TGCA 6 cut(s) 12, 696, 727, 1037, 1277, 1507
HpyF10VI GCNNNNNNNGC 1 cut(s) 1501
HpyF3I CTNAG 6 cut(s) 423, 756, 1157, 1464, 1491, 1554
HpySE526I ACGT 2 cut(s) 646, 1047
Hsp92II CATG 9 cut(s) 262, 359, 444, 570, 1022, 1055, 1178, 1340, 1355
HspAI GCGC 1 cut(s) 884
Ksp22I TGATCA 2 cut(s) 781, 997
Kzo9I GATC 5 cut(s) 463, 781, 997, 1411, 1566
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 6 cut(s) 55, 85, 352, 405, 881, 1189
Lsp1109I GCAGC 3 cut(s) 380, 1447, 1491
LweI GCATC 4 cut(s) 1264, 1396, 1449, 1620
MaeI CTAG 1 cut(s) 990
MaeII ACGT 2 cut(s) 646, 1047
MaeIII GTNAC 4 cut(s) 214, 476, 499, 1486
MalI GATC 5 cut(s) 465, 783, 999, 1413, 1568
MboI GATC 5 cut(s) 463, 781, 997, 1411, 1566
MboII GAAGA 8 cut(s) 365, 608, 754, 814, 846, 862, 1368, 1684
MfeI CAATTG 2 cut(s) 921, 1678
MhlI GDGCHC 1 cut(s) 410
MlyI GAGTC 1 cut(s) 1427
MmeI TCCRAC 2 cut(s) 507, 1117
Mph1103I ATGCAT 1 cut(s) 1279
MseI TTAA 3 cut(s) 591, 1113, 1592
MslI CAYNNNNRTG 7 cut(s) 300, 398, 400, 834, 1091, 1179, 1257
MspA1I CMGCKG 2 cut(s) 341, 1257
MspI CCGG 1 cut(s) 53
MspR9I CCNGG 4 cut(s) 102, 513, 948, 1497
MunI CAATTG 2 cut(s) 921, 1678
Mva1269I GAATGC 1 cut(s) 774
MvaI CCWGG 4 cut(s) 102, 513, 948, 1497
MwoI GCNNNNNNNGC 1 cut(s) 1501
NdeI CATATG 1 cut(s) 1279
NdeII GATC 5 cut(s) 463, 781, 997, 1411, 1566
NlaIII CATG 9 cut(s) 262, 359, 444, 570, 1022, 1055, 1178, 1340, 1355
NlaIV GGNNCC 3 cut(s) 57, 407, 1527
NsiI ATGCAT 1 cut(s) 1279
NspI RCATGY 5 cut(s) 359, 570, 1178, 1340, 1355
NspV TTCGAA 2 cut(s) 1204, 1619
OliI CACNNNNGTG 2 cut(s) 398, 1257
PaeI GCATGC 1 cut(s) 1355
PaeR7I CTCGAG 1 cut(s) 1435
PciI ACATGT 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 378
PctI GAATGC 1 cut(s) 774
PfeI GAWTC 4 cut(s) 28, 174, 745, 751
PflFI GACNNNGTC 1 cut(s) 645
PflMI CCANNNNNTGG 1 cut(s) 1067
PkrI GCNGC 5 cut(s) 370, 384, 935, 1462, 1506
PleI GAGTC 1 cut(s) 1427
PpsI GAGTC 1 cut(s) 1427
PpuMI RGGWCCY 2 cut(s) 721, 1652
PscI ACATGT 1 cut(s) 566
PsiI TTATAA 1 cut(s) 225
Psp5II RGGWCCY 2 cut(s) 721, 1652
Psp6I CCWGG 4 cut(s) 100, 511, 946, 1495
PspFI CCCAGC 1 cut(s) 1355
PspGI CCWGG 4 cut(s) 100, 511, 946, 1495
PspN4I GGNNCC 3 cut(s) 57, 407, 1527
PspPI GGNCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
PspPPI RGGWCCY 2 cut(s) 721, 1652
PspXI VCTCGAGB 1 cut(s) 1435
PstI CTGCAG 3 cut(s) 698, 729, 1509
PsyI GACNNNGTC 1 cut(s) 645
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 4 cut(s) 601, 685, 893, 1292
RsaNI GTAC 4 cut(s) 600, 684, 892, 1291
RseI CAYNNNNRTG 7 cut(s) 300, 398, 400, 834, 1091, 1179, 1257
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 3 cut(s) 591, 1113, 1592
SatI GCNGC 5 cut(s) 369, 383, 934, 1461, 1505
Sau3AI GATC 5 cut(s) 463, 781, 997, 1411, 1566
Sau96I GGNCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
SchI GAGTC 1 cut(s) 1427
ScrFI CCNGG 4 cut(s) 102, 513, 948, 1497
SduI GDGCHC 1 cut(s) 410
SfaNI GCATC 4 cut(s) 1264, 1396, 1449, 1620
SfcI CTRYAG 6 cut(s) 22, 678, 694, 725, 1505, 1656
Sfr274I CTCGAG 1 cut(s) 1435
SfuI TTCGAA 2 cut(s) 1204, 1619
SinI GGWCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
SlaI CTCGAG 1 cut(s) 1435
SmiMI CAYNNNNRTG 7 cut(s) 300, 398, 400, 834, 1091, 1179, 1257
SmlI CTYRAG 1 cut(s) 1435
SmoI CTYRAG 1 cut(s) 1435
SphI GCATGC 1 cut(s) 1355
SsiI CCGC 4 cut(s) 383, 933, 1257, 1349
SspMI CTAG 1 cut(s) 990
StyD4I CCNGG 4 cut(s) 100, 511, 946, 1495
TaaI ACNGT 2 cut(s) 284, 820
TaiI ACGT 2 cut(s) 649, 1050
TaqI TCGA 6 cut(s) 316, 376, 1204, 1322, 1436, 1619
TatI WGTACW 2 cut(s) 891, 1290
TauI GCSGC 2 cut(s) 385, 936
TfiI GAWTC 4 cut(s) 28, 174, 745, 751
Tru1I TTAA 3 cut(s) 591, 1113, 1592
Tru9I TTAA 3 cut(s) 591, 1113, 1592
TscAI CASTG 5 cut(s) 237, 710, 864, 1423, 1608
TseI GCWGC 3 cut(s) 368, 1460, 1504
TspDTI ATGAA 7 cut(s) 429, 546, 779, 818, 942, 1040, 1464
TspRI CASTG 5 cut(s) 237, 710, 864, 1423, 1608
Tth111I GACNNNGTC 1 cut(s) 645
Van91I CCANNNNNTGG 1 cut(s) 1067
VpaK11BI GGWCC 8 cut(s) 180, 721, 812, 1118, 1139, 1526, 1652, 1661
XapI RAATTY 5 cut(s) 164, 237, 786, 940, 1623
XceI RCATGY 5 cut(s) 359, 570, 1178, 1340, 1355
XcmI CCANNNNNNNNNTGG 1 cut(s) 90
XhoI CTCGAG 1 cut(s) 1435
XmiI GTMKAC 2 cut(s) 843, 1539
XspI CTAG 1 cut(s) 990
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.