MD05G1189000.v1.1

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007670
Physical Location & Seq
Reverse (-)
31667578 .. 31671188
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1189000.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGTTCGGGTGCAAGTGCTTCCATTGGAATCAGCTCACAGATTTATTACCACCTGAGCCTGAAGAACCCTTCTCTCTGCCTCATCCCATTCCTCAATGGCCCCCAGGTGAAGGTTTTGCTTCTGGAAGAATCAATCTCGGAGAAATAGAGGTTTCCAAGATAACCAGATTTGAGTTTATCTGGGCCTGCACTCCATCAGAGGACAAGAAAAAACATGTTACGTTTTATAAGCCAGCAGGAATACCTGATGGATTCCATAGCATTGGTCATTATTGCCAATCCAAGGATAAACCTTTACACGGTTTTGTTCTTGTGGTTAAAGAGGCAGAGTTTCCAGAAACAGCTGATGTTGGGGAGAGTGTCAAGTTGCCGGCTCTCAGAGAGCCACTTGATTACACGTTAGTATGGAGCCCTGATGACGGGAATGGGGACAATCATGGTGCATATGGTTATTTTTGGCATCCTCAGCCACCAGAAGGTTACAAAGCAATGGGCTTTTTAGTGACTAACAGGCTTGATAAACCTCGGTTGGATGAAGTGAGGTGCGTTCGAGCTGATTTGACTGACAGATGTGAAACTTACCGCCCAGTTCTTAATGCGATTACAAAATCTCTAAATCTTCCTTTCCAAGTTTGGACTACAAGACCACATCATCGGGGAATGATGGGGAAAGGGGTTTCTGCCGGTACATTTTTCTGCACTAGTGACTCCAACGTTGGAAAGGATGTGCATATTGGATGCTTGAAGAATTTGAATCCGAAGCTGCCTGGAATGCCAAACCTTGATCAAATTCATTCGCTTATCCATCATTATGGACCCACCGTATTTTTTCATCCAGAAGAGATTTACTTGCCATCTTCTGTTTCATGGTTCTTCAAAAGTGGAGCGCTTTTGCACAAAACTGGCACGTCGGATGGTGAGGCTATTGATGGTAGTGGCTCAAATTTGCCTGGCGGGGGGTCAAATGATGGGGGGTTTTGGATTGACTTGCCGACTGGTGAACGAAGAGAGATAATAAAACGCGGAAACTTGGAAAGTGCAGAACTTTATGTTCATGTGAAACCGGCTCTTGGTGGGACTTTCACTGACATTGCAATGTGGGTGTTTTGTCCCTTCAATGGACCAGCCACTCTCAAAGTTGGGCCTCTGGACTTCCCTCTTAGCAAGATCGGACAGCATGTCGGTGACTGGGAGCATTTCACACTCCGCATCTGCAACTTTTCTGGAGAGCTCCAGAGCATATACTTCTCGGAGCACAGCGGTGGCAAATGGGTGGATGCATATGATTTGGAGTACACAGACGGGAACAAAGCCATTGTCTACTTATCCAAAAATGGACATGCGAGCTACCCTCATCCCGGGACTTTCCTTCAGGGGTCAGACAAGCTAGGCATCGGAATAAGGAACGATGCTGCTCGCAGCAACTTATCTGTGGATTCAAGCATTCGGTATGAGATTGTTGCAGCCGAGTATCTTGGAAACGGGGTTGTGAGTGAGCCGGATTGGCTGCAGTTTATGAGAGAGTGGGGTCCAACAATAGTGTACAATTCAAGAACTGAGCTTGATAAGGTTATCAATGTCTTGCCTGTGGTGTTTAAGTACAGATTGGAGAACATATTTAATAAGCTTCCAGTTGAGCTTTATGGGGAGGAAGGTCCTACTGGACCAAAGGAGAAGAACAATTGGGTGGGAGATGAGAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

569

Amino Acids

63.41

Weight (kDa)

5.76

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 21 - 566 6.3e-263 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AccI GTMKAC 1 cut(s) 1320
AccII CGCG 1 cut(s) 1023
AciI CCGC 5 cut(s) 583, 954, 1023, 1207, 1260
AclI AACGTT 1 cut(s) 714
AcsI RAATTY 3 cut(s) 748, 789, 943
AcuI CTGAAG 2 cut(s) 81, 1355
AfaI GTAC 4 cut(s) 688, 1295, 1544, 1601
AfeI AGCGCT 1 cut(s) 888
AfiI CCNNNNNNNGG 9 cut(s) 110, 283, 299, 419, 476, 956, 1070, 1118, 1358
AflIII ACRYGT 2 cut(s) 214, 396
AgsI TTSAA 6 cut(s) 745, 754, 877, 1117, 1440, 1551
AhdI GACNNNNNGTC 1 cut(s) 1178
AhlI ACTAGT 1 cut(s) 701
AjiI CACGTC 1 cut(s) 909
AjnI CCWGG 3 cut(s) 103, 766, 949
AleI CACNNNNGTG 1 cut(s) 1260
Alw21I GWGCWC 2 cut(s) 1233, 1257
Ama87I CYCGRG 1 cut(s) 1358
Aor51HI AGCGCT 1 cut(s) 888
AoxI GGCC 3 cut(s) 98, 183, 1142
ApeKI GCWGC 5 cut(s) 763, 1412, 1419, 1463, 1507
ApoI RAATTY 3 cut(s) 748, 789, 943
ArsI GACNNNNNNTTYG 2 cut(s) 1302, 1334
AspLEI GCGC 1 cut(s) 889
AspS9I GGNCC 8 cut(s) 99, 183, 815, 1121, 1142, 1529, 1655, 1664
AsuC2I CCSGG 2 cut(s) 1359, 1360
AsuHPI GGTGA 4 cut(s) 119, 929, 1010, 1196
AvaI CYCGRG 1 cut(s) 1358
AvaII GGWCC 5 cut(s) 815, 1121, 1529, 1655, 1664
BanII GRGCYC 2 cut(s) 413, 1233
Bbv12I GWGCWC 2 cut(s) 1233, 1257
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 5 cut(s) 750, 1399, 1431, 1475, 1494
BccI CCATC 8 cut(s) 202, 242, 658, 813, 862, 908, 923, 962
BciT130I CCWGG 3 cut(s) 105, 768, 951
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 2 cut(s) 1359, 1360
BcuI ACTAGT 1 cut(s) 701
BfaI CTAG 3 cut(s) 702, 1388, 1705
BfmI CTRYAG 1 cut(s) 1508
BfoI RGCGCY 1 cut(s) 890
BglI GCCNNNNNGGC 1 cut(s) 1504
BisI GCNGC 5 cut(s) 764, 1413, 1420, 1464, 1508
BlsI GCNGC 5 cut(s) 765, 1414, 1421, 1465, 1509
Bme1390I CCNGG 5 cut(s) 105, 768, 951, 1359, 1360
Bme18I GGWCC 5 cut(s) 815, 1121, 1529, 1655, 1664
BmeRI GACNNNNNGTC 1 cut(s) 1178
BmeT110I CYCGRG 1 cut(s) 1358
BmgBI CACGTC 1 cut(s) 909
BmgT120I GGNCC 8 cut(s) 99, 183, 815, 1121, 1142, 1529, 1655, 1664
BmiI GGNNCC 4 cut(s) 101, 410, 817, 1530
BmrFI CCNGG 5 cut(s) 105, 768, 951, 1359, 1360
BmrI ACTGGG 2 cut(s) 581, 1198
BmsI GCATC 6 cut(s) 469, 728, 1218, 1267, 1399, 1401
BmuI ACTGGG 2 cut(s) 581, 1198
BplI GAGNNNNNCTC 2 cut(s) 1336, 1368
BpmI CTGGAG 2 cut(s) 1217, 1245
Bpu10I CCTNAGC 2 cut(s) 54, 465
BpuMI CCSGG 2 cut(s) 1359, 1360
BsaJI CCNNGG 4 cut(s) 103, 282, 524, 1358
Bsc4I CCNNNNNNNGG 9 cut(s) 110, 283, 299, 419, 476, 956, 1070, 1118, 1358
Bse118I RCCGGY 3 cut(s) 370, 683, 1063
Bse1I ACTGG 6 cut(s) 587, 907, 1000, 1193, 1631, 1666
Bse3DI GCAATG 3 cut(s) 495, 1089, 1101
BseBI CCWGG 3 cut(s) 105, 768, 951
BseDI CCNNGG 4 cut(s) 103, 282, 524, 1358
BseGI GGATG 9 cut(s) 82, 460, 538, 730, 743, 832, 919, 1282, 1354
BseLI CCNNNNNNNGG 9 cut(s) 110, 283, 299, 419, 476, 956, 1070, 1118, 1358
BseMI GCAATG 3 cut(s) 495, 1089, 1101
BseMII CTCAG 4 cut(s) 45, 391, 479, 1548
BseNI ACTGG 6 cut(s) 587, 907, 1000, 1193, 1631, 1666
BseXI GCAGC 5 cut(s) 750, 1399, 1431, 1475, 1494
BsgI GTGCAG 3 cut(s) 172, 682, 1059
Bsh1236I CGCG 1 cut(s) 1023
BshFI GGCC 3 cut(s) 100, 185, 1144
BsiHKAI GWGCWC 2 cut(s) 1233, 1257
BsiHKCI CYCGRG 1 cut(s) 1358
BsiSI CCGG 5 cut(s) 371, 684, 1064, 1359, 1499
BslFI GGGAC 4 cut(s) 443, 1090, 1095, 1375
BslI CCNNNNNNNGG 9 cut(s) 110, 283, 299, 419, 476, 956, 1070, 1118, 1358
BsmFI GGGAC 4 cut(s) 443, 1090, 1095, 1375
BsmI GAATGC 2 cut(s) 777, 1443
BsnI GGCC 3 cut(s) 100, 185, 1144
BsoBI CYCGRG 1 cut(s) 1358
Bsp1286I GDGCHC 3 cut(s) 413, 1233, 1257
Bsp1407I TGTACA 1 cut(s) 1542
Bsp143I GATC 2 cut(s) 784, 1167
BspACI CCGC 5 cut(s) 583, 954, 1023, 1207, 1260
BspANI GGCC 3 cut(s) 100, 185, 1144
BspCNI CTCAG 4 cut(s) 46, 390, 478, 1549
BspFNI CGCG 1 cut(s) 1023
BspLI GGNNCC 4 cut(s) 101, 410, 817, 1530
BspMAI CTGCAG 1 cut(s) 1512
BsrDI GCAATG 3 cut(s) 495, 1089, 1101
BsrFI RCCGGY 3 cut(s) 370, 683, 1063
BsrGI TGTACA 1 cut(s) 1542
BsrI ACTGG 6 cut(s) 587, 907, 1000, 1193, 1631, 1666
BssAI RCCGGY 3 cut(s) 370, 683, 1063
BssECI CCNNGG 4 cut(s) 103, 282, 524, 1358
BssMI GATC 2 cut(s) 784, 1167
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 3 cut(s) 105, 768, 951
Bst4CI ACNGT 2 cut(s) 302, 823
Bst6I CTCTTC 2 cut(s) 834, 1000
BstAUI TGTACA 1 cut(s) 1542
BstC8I GCNNGC 5 cut(s) 187, 234, 372, 1345, 1417
BstDEI CTNAG 5 cut(s) 54, 377, 465, 1160, 1557
BstF5I GGATG 9 cut(s) 82, 460, 538, 730, 743, 832, 919, 1282, 1354
BstFNI CGCG 1 cut(s) 1023
BstH2I RGCGCY 1 cut(s) 890
BstHHI GCGC 1 cut(s) 889
BstKTI GATC 2 cut(s) 787, 1170
BstMBI GATC 2 cut(s) 784, 1167
BstMWI GCNNNNNNNGC 3 cut(s) 466, 772, 1504
BstNI CCWGG 3 cut(s) 105, 768, 951
BstNSI RCATGY 3 cut(s) 218, 1181, 1343
BstSCI CCNGG 5 cut(s) 103, 766, 949, 1357, 1358
BstSFI CTRYAG 1 cut(s) 1508
BstUI CGCG 1 cut(s) 1023
BstV1I GCAGC 5 cut(s) 750, 1399, 1431, 1475, 1494
BstXI CCANNNNNNTGG 2 cut(s) 263, 812
BsuRI GGCC 3 cut(s) 100, 185, 1144
BtrI CACGTC 1 cut(s) 909
BtsCI GGATG 9 cut(s) 82, 460, 538, 730, 743, 832, 919, 1282, 1354
BtsIMutI CAGTG 1 cut(s) 1083
Cac8I GCNNGC 5 cut(s) 187, 234, 372, 1345, 1417
CfoI GCGC 1 cut(s) 889
Cfr10I RCCGGY 3 cut(s) 370, 683, 1063
Cfr13I GGNCC 8 cut(s) 99, 183, 815, 1121, 1142, 1529, 1655, 1664
Cfr9I CCCGGG 1 cut(s) 1358
Csp6I GTAC 4 cut(s) 687, 1294, 1543, 1600
CviAII CATG 6 cut(s) 215, 437, 867, 1055, 1178, 1340
CviQI GTAC 4 cut(s) 687, 1294, 1543, 1600
DdeI CTNAG 5 cut(s) 54, 377, 465, 1160, 1557
DpnI GATC 2 cut(s) 786, 1169
DpnII GATC 2 cut(s) 784, 1167
DriI GACNNNNNGTC 1 cut(s) 1178
Eam1104I CTCTTC 2 cut(s) 834, 1000
Eam1105I GACNNNNNGTC 1 cut(s) 1178
EarI CTCTTC 2 cut(s) 834, 1000
Ecl136II GAGCTC 1 cut(s) 1231
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 2 cut(s) 413, 1233
Eco47I GGWCC 5 cut(s) 815, 1121, 1529, 1655, 1664
Eco47III AGCGCT 1 cut(s) 888
Eco53kI GAGCTC 1 cut(s) 1231
Eco57I CTGAAG 2 cut(s) 81, 1355
Eco88I CYCGRG 1 cut(s) 1358
EcoICRI GAGCTC 1 cut(s) 1231
EcoO109I RGGNCCY 1 cut(s) 1655
EcoRII CCWGG 3 cut(s) 103, 766, 949
EcoT14I CCWWGG 1 cut(s) 282
EcoT22I ATGCAT 1 cut(s) 1282
EcoT38I GRGCYC 2 cut(s) 413, 1233
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 6 cut(s) 218, 440, 870, 1058, 1181, 1343
FaqI GGGAC 4 cut(s) 443, 1090, 1095, 1375
FatI CATG 6 cut(s) 214, 436, 866, 1054, 1177, 1339
FauI CCCGC 1 cut(s) 947
FauNDI CATATG 2 cut(s) 445, 1282
FbaI TGATCA 1 cut(s) 784
FblI GTMKAC 1 cut(s) 1320
Fnu4HI GCNGC 5 cut(s) 764, 1413, 1420, 1464, 1508
FokI GGATG 9 cut(s) 69, 447, 545, 737, 750, 819, 926, 1289, 1341
FriOI GRGCYC 2 cut(s) 413, 1233
Fsp4HI GCNGC 5 cut(s) 764, 1413, 1420, 1464, 1508
FspBI CTAG 3 cut(s) 702, 1388, 1705
GlaI GCGC 1 cut(s) 888
GluI GCNGC 5 cut(s) 764, 1413, 1420, 1464, 1508
GsuI CTGGAG 2 cut(s) 1217, 1245
HaeII RGCGCY 1 cut(s) 890
HaeIII GGCC 3 cut(s) 100, 185, 1144
HapII CCGG 5 cut(s) 371, 684, 1064, 1359, 1499
HhaI GCGC 1 cut(s) 889
Hin1II CATG 6 cut(s) 218, 440, 870, 1058, 1181, 1343
Hin6I GCGC 1 cut(s) 887
HinP1I GCGC 1 cut(s) 887
HindIII AAGCTT 1 cut(s) 1625
HinfI GANTC 6 cut(s) 28, 129, 252, 707, 754, 1436
HpaII CCGG 5 cut(s) 371, 684, 1064, 1359, 1499
HphI GGTGA 4 cut(s) 119, 929, 1010, 1196
Hpy166II GTNNAC 4 cut(s) 1001, 1296, 1321, 1543
Hpy188I TCNGA 9 cut(s) 140, 199, 380, 759, 913, 1172, 1252, 1381, 1397
Hpy188III TCNNGA 7 cut(s) 123, 335, 836, 1148, 1224, 1234, 1551
Hpy8I GTNNAC 4 cut(s) 1001, 1296, 1321, 1543
Hpy99I CGWCG 1 cut(s) 913
HpyAV CCTTC 6 cut(s) 79, 104, 470, 1123, 1379, 1646
HpyCH4III ACNGT 2 cut(s) 302, 823
HpyCH4IV ACGT 4 cut(s) 221, 398, 714, 908
HpyF10VI GCNNNNNNNGC 3 cut(s) 466, 772, 1504
HpyF3I CTNAG 5 cut(s) 54, 377, 465, 1160, 1557
HpySE526I ACGT 4 cut(s) 221, 398, 714, 908
Hsp92II CATG 6 cut(s) 218, 440, 870, 1058, 1181, 1343
HspAI GCGC 1 cut(s) 887
KroI GCCGGC 1 cut(s) 370
KroNI GCCGGC 1 cut(s) 372
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 2 cut(s) 784, 1167
LmnI GCTCC 5 cut(s) 408, 884, 1192, 1236, 1252
Lsp1109I GCAGC 5 cut(s) 750, 1399, 1431, 1475, 1494
LweI GCATC 6 cut(s) 469, 728, 1218, 1267, 1399, 1401
MaeI CTAG 3 cut(s) 702, 1388, 1705
MaeII ACGT 4 cut(s) 221, 398, 714, 908
MaeIII GTNAC 5 cut(s) 217, 479, 502, 704, 1184
MalI GATC 2 cut(s) 786, 1169
MboI GATC 2 cut(s) 784, 1167
MboII GAAGA 9 cut(s) 74, 138, 611, 757, 849, 851, 865, 1017, 1687
MfeI CAATTG 1 cut(s) 1681
MhlI GDGCHC 3 cut(s) 413, 1233, 1257
MluCI AATT 5 cut(s) 748, 789, 943, 1546, 1681
MlyI GAGTC 1 cut(s) 701
MmeI TCCRAC 5 cut(s) 510, 697, 735, 891, 1556
Mph1103I ATGCAT 1 cut(s) 1282
MroNI GCCGGC 1 cut(s) 370
MseI TTAA 4 cut(s) 318, 594, 1596, 1620
MslI CAYNNNNRTG 4 cut(s) 810, 1094, 1182, 1260
MspA1I CMGCKG 2 cut(s) 344, 1260
MspI CCGG 5 cut(s) 371, 684, 1064, 1359, 1499
MspR9I CCNGG 5 cut(s) 105, 768, 951, 1359, 1360
MunI CAATTG 1 cut(s) 1681
Mva1269I GAATGC 2 cut(s) 777, 1443
MvaI CCWGG 3 cut(s) 105, 768, 951
MvnI CGCG 1 cut(s) 1023
MwoI GCNNNNNNNGC 3 cut(s) 466, 772, 1504
NaeI GCCGGC 1 cut(s) 372
NciI CCSGG 2 cut(s) 1359, 1360
NdeI CATATG 2 cut(s) 445, 1282
NdeII GATC 2 cut(s) 784, 1167
NgoMIV GCCGGC 1 cut(s) 370
NlaIII CATG 6 cut(s) 218, 440, 870, 1058, 1181, 1343
NlaIV GGNNCC 4 cut(s) 101, 410, 817, 1530
NmeAIII GCCGAG 1 cut(s) 1492
NmuCI GTSAC 3 cut(s) 502, 704, 1184
NsiI ATGCAT 1 cut(s) 1282
NspI RCATGY 3 cut(s) 218, 1181, 1343
OliI CACNNNNGTG 1 cut(s) 1260
PciI ACATGT 1 cut(s) 214
PctI GAATGC 2 cut(s) 777, 1443
PdiI GCCGGC 1 cut(s) 372
PfeI GAWTC 5 cut(s) 28, 129, 252, 754, 1436
PkrI GCNGC 5 cut(s) 765, 1414, 1421, 1465, 1509
PleI GAGTC 1 cut(s) 701
PpsI GAGTC 1 cut(s) 701
PpuMI RGGWCCY 1 cut(s) 1655
PscI ACATGT 1 cut(s) 214
PsiI TTATAA 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 1233
Psp1406I AACGTT 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 1655
Psp6I CCWGG 3 cut(s) 103, 766, 949
PspGI CCWGG 3 cut(s) 103, 766, 949
PspN4I GGNNCC 4 cut(s) 101, 410, 817, 1530
PspPI GGNCC 8 cut(s) 99, 183, 815, 1121, 1142, 1529, 1655, 1664
PspPPI RGGWCCY 1 cut(s) 1655
PstI CTGCAG 1 cut(s) 1512
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 4 cut(s) 688, 1295, 1544, 1601
RsaNI GTAC 4 cut(s) 687, 1294, 1543, 1600
RseI CAYNNNNRTG 4 cut(s) 810, 1094, 1182, 1260
SacI GAGCTC 1 cut(s) 1233
SaqAI TTAA 4 cut(s) 318, 594, 1596, 1620
SatI GCNGC 5 cut(s) 764, 1413, 1420, 1464, 1508
Sau3AI GATC 2 cut(s) 784, 1167
Sau96I GGNCC 8 cut(s) 99, 183, 815, 1121, 1142, 1529, 1655, 1664
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 5 cut(s) 105, 768, 951, 1359, 1360
SduI GDGCHC 3 cut(s) 413, 1233, 1257
SfaNI GCATC 6 cut(s) 469, 728, 1218, 1267, 1399, 1401
SfcI CTRYAG 1 cut(s) 1508
SinI GGWCC 5 cut(s) 815, 1121, 1529, 1655, 1664
SmaI CCCGGG 1 cut(s) 1360
SmiMI CAYNNNNRTG 4 cut(s) 810, 1094, 1182, 1260
SpeI ACTAGT 1 cut(s) 701
Sse9I AATT 5 cut(s) 748, 789, 943, 1546, 1681
SsiI CCGC 5 cut(s) 583, 954, 1023, 1207, 1260
SspMI CTAG 3 cut(s) 702, 1388, 1705
SstI GAGCTC 1 cut(s) 1233
StyD4I CCNGG 5 cut(s) 103, 766, 949, 1357, 1358
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 302, 823
TaiI ACGT 4 cut(s) 224, 401, 717, 911
TaqI TCGA 1 cut(s) 550
TasI AATT 5 cut(s) 748, 789, 943, 1546, 1681
TatI WGTACW 3 cut(s) 1293, 1542, 1599
TfiI GAWTC 5 cut(s) 28, 129, 252, 754, 1436
Tru1I TTAA 4 cut(s) 318, 594, 1596, 1620
Tru9I TTAA 4 cut(s) 318, 594, 1596, 1620
TscAI CASTG 1 cut(s) 1090
TseFI GTSAC 3 cut(s) 502, 704, 1184
TseI GCWGC 5 cut(s) 763, 1412, 1419, 1463, 1507
Tsp45I GTSAC 3 cut(s) 502, 704, 1184
TspDTI ATGAA 5 cut(s) 549, 782, 821, 855, 1043
TspMI CCCGGG 1 cut(s) 1358
TspRI CASTG 1 cut(s) 1090
VpaK11BI GGWCC 5 cut(s) 815, 1121, 1529, 1655, 1664
XapI RAATTY 3 cut(s) 748, 789, 943
XceI RCATGY 3 cut(s) 218, 1181, 1343
XmaI CCCGGG 1 cut(s) 1358
XmiI GTMKAC 1 cut(s) 1320
XspI CTAG 3 cut(s) 702, 1388, 1705
Zsp2I ATGCAT 1 cut(s) 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.