AT5G44450

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011459
Physical Location & Seq
Reverse (-)
17913997 .. 17916019
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44450.1

Sequence Viewer

Length: 831 bp
ATGGATATTTGTGGCGTTGATTCCGAAGGGAAAGAGTTTAATAGCGTACAAGAGATGTGGAGAGAAGAAATTGGAGAAGGAGACGAAACTAAAAAAACCCAATGGTATAGAGATGGTGTTTCTTATTGGGAAGGTGTTGAAGCTTCTGTTGATGGAGTATTAGGAGGATATGGGCATGTGAATGATGCTGATATTATAGGAAGTGAGGTTTTTCTTAAGACTCTTTTGCAAGAAAGATTAGTTAACAATGTTGGAGCTAATCAGCATTTAGTAGCTCTTGATTGTGGATCTGGTATTGGAAGAATCACTAAAAATCTTCTGATTCGATACTTTAACGAGGTTGATCTTCTTGAGCCTGTAGCACAATTTTTGGATGCAGCTCGTGAAAATTTGGCATCTGCAGGCTCTGAAACGCACAAAGCTACTAACTTTTTCTGTGTACCTCTTCAGGAGTTTACTCCAGCTGCTGGGAGGTATGATGTAATATGGGTTCAATGGTGCATTGGGCATCTCACGGACAATGACTTTGTTTCTTTCTTTAACCGCGCAAAGGGATACCTTAAACCTGGTGGGTTTTTCGTCGTGAAAGAGAATCTCGCTAAAAATGGATTTGTTTTGGATAAAGAGGATCATAGCATCACCAGATCGGATCCATACTTCAAACAGCTCTTTCGCCAATGTGGGTTACATCTCTATAGAACAAAGGATCAGAAAGGTCTTCCACAAGAGTTATTTGCTGTAAAAATGTATGCGTTAACGGTTGACACACCACCCAAAATCCACCGAACCAGGTCAAAAACTCGAAGCAACAGACCGCAGATTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

276

Amino Acids

31.25

Weight (kDa)

6.01

Isoelectric Point (pI)

24.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_PK PF05891 32 - 253 3.6e-101 AdoMet dependent proline di-methyltransferase
Methyltransf_25 PF13649 93 - 191 5.1e-11 Methyltransferase domain
Methyltransf_11 PF08241 93 - 195 1e-09 Methyltransferase domain
Methyltransf_12 PF08242 93 - 193 3.1e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 467
AccII CGCG 1 cut(s) 546
AciI CCGC 2 cut(s) 544, 815
AclWI GGATC 5 cut(s) 295, 636, 644, 657, 714
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 2 cut(s) 48, 441
AfiI CCNNNNNNNGG 2 cut(s) 467, 550
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 3 cut(s) 140, 494, 661
AjnI CCWGG 2 cut(s) 565, 788
AluBI AGCT 7 cut(s) 143, 257, 275, 380, 422, 464, 667
AluI AGCT 7 cut(s) 143, 257, 275, 380, 422, 464, 667
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 5 cut(s) 295, 636, 644, 657, 714
AlwNI CAGNNNCTG 2 cut(s) 407, 467
ApeKI GCWGC 2 cut(s) 377, 464
ApoI RAATTY 1 cut(s) 388
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
AspLEI GCGC 1 cut(s) 548
AsuHPI GGTGA 1 cut(s) 631
BamHI GGATCC 1 cut(s) 649
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 710
BbvI GCAGC 2 cut(s) 389, 451
BccI CCATC 2 cut(s) 107, 146
BciT130I CCWGG 2 cut(s) 567, 790
BciVI GTATCC 1 cut(s) 548
BcoDI GTCTC 1 cut(s) 75
BfmI CTRYAG 3 cut(s) 357, 399, 694
BfrI CTTAAG 1 cut(s) 215
BfuI GTATCC 1 cut(s) 548
BisI GCNGC 2 cut(s) 378, 465
BlsI GCNGC 2 cut(s) 379, 466
Bme1390I CCNGG 2 cut(s) 567, 790
BmiI GGNNCC 1 cut(s) 651
BmrFI CCNGG 2 cut(s) 567, 790
BmsI GCATC 5 cut(s) 175, 364, 404, 517, 645
BpiI GAAGAC 1 cut(s) 710
BpmI CTGGAG 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 371
Bsc4I CCNNNNNNNGG 2 cut(s) 467, 550
BseBI CCWGG 2 cut(s) 567, 790
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 2 cut(s) 467, 550
BseXI GCAGC 2 cut(s) 389, 451
BseYI CCCAGC 1 cut(s) 467
Bsh1236I CGCG 1 cut(s) 546
BslI CCNNNNNNNGG 2 cut(s) 467, 550
BsmAI GTCTC 1 cut(s) 75
BsmBI CGTCTC 1 cut(s) 75
Bsp143I GATC 6 cut(s) 287, 343, 628, 644, 649, 706
BspACI CCGC 2 cut(s) 544, 815
BspFNI CGCG 1 cut(s) 546
BspLI GGNNCC 1 cut(s) 651
BspMAI CTGCAG 1 cut(s) 403
BspPI GGATC 5 cut(s) 295, 636, 644, 657, 714
BspTI CTTAAG 1 cut(s) 215
BssMI GATC 6 cut(s) 287, 343, 628, 644, 649, 706
BssSI CACGAG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 2 cut(s) 567, 790
Bst4CI ACNGT 1 cut(s) 760
Bst6I CTCTTC 1 cut(s) 450
BstAFI CTTAAG 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 403
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 546
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 6 cut(s) 290, 346, 631, 647, 652, 709
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 6 cut(s) 287, 343, 628, 644, 649, 706
BstNI CCWGG 2 cut(s) 567, 790
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 2 cut(s) 565, 788
BstSFI CTRYAG 3 cut(s) 357, 399, 694
BstUI CGCG 1 cut(s) 546
BstV1I GCAGC 2 cut(s) 389, 451
BstV2I GAAGAC 1 cut(s) 710
BstX2I RGATCY 2 cut(s) 287, 649
BstYI RGATCY 2 cut(s) 287, 649
BsuI GTATCC 1 cut(s) 548
BtsCI GGATG 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 403
CaiI CAGNNNCTG 2 cut(s) 407, 467
CfoI GCGC 1 cut(s) 548
CsiI ACCWGGT 2 cut(s) 565, 788
Csp6I GTAC 2 cut(s) 47, 440
CspCI CAANNNNNGTGG 2 cut(s) 38, 73
CviAII CATG 1 cut(s) 176
CviJI RGCY 9 cut(s) 143, 257, 275, 355, 380, 405, 422, 464, 667
CviKI_1 RGCY 9 cut(s) 143, 257, 275, 355, 380, 405, 422, 464, 667
CviQI GTAC 2 cut(s) 47, 440
DpnI GATC 6 cut(s) 289, 345, 630, 646, 651, 708
DpnII GATC 6 cut(s) 287, 343, 628, 644, 649, 706
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 565, 788
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 1 cut(s) 179
FatI CATG 1 cut(s) 175
Fnu4HI GCNGC 2 cut(s) 378, 465
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 2 cut(s) 378, 465
GlaI GCGC 1 cut(s) 547
GluI GCNGC 2 cut(s) 378, 465
GsaI CCCAGC 1 cut(s) 471
GsuI CTGGAG 1 cut(s) 444
HhaI GCGC 1 cut(s) 548
Hin1II CATG 1 cut(s) 179
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 3 cut(s) 244, 756, 763
HindII GTYRAC 3 cut(s) 244, 756, 763
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 5 cut(s) 20, 220, 303, 322, 592
HpaI GTTAAC 2 cut(s) 244, 756
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 5 cut(s) 244, 440, 456, 756, 763
Hpy188I TCNGA 5 cut(s) 25, 321, 409, 649, 711
Hpy188III TCNNGA 5 cut(s) 278, 350, 383, 449, 583
Hpy8I GTNNAC 5 cut(s) 244, 440, 456, 756, 763
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 3 cut(s) 20, 71, 125
HpyCH4III ACNGT 1 cut(s) 760
HpyCH4V TGCA 4 cut(s) 229, 377, 401, 501
Hsp92II CATG 1 cut(s) 179
HspAI GCGC 1 cut(s) 546
KspAI GTTAAC 2 cut(s) 244, 756
Kzo9I GATC 6 cut(s) 287, 343, 628, 644, 649, 706
LmnI GCTCC 1 cut(s) 254
Lsp1109I GCAGC 2 cut(s) 389, 451
LweI GCATC 5 cut(s) 175, 364, 404, 517, 645
MabI ACCWGGT 2 cut(s) 565, 788
MaeIII GTNAC 1 cut(s) 684
MalI GATC 6 cut(s) 289, 345, 630, 646, 651, 708
MboI GATC 6 cut(s) 287, 343, 628, 644, 649, 706
MboII GAAGA 6 cut(s) 77, 308, 312, 338, 437, 710
MflI RGATCY 2 cut(s) 287, 649
MluCI AATT 3 cut(s) 69, 365, 388
MlyI GAGTC 1 cut(s) 214
MmeI TCCRAC 1 cut(s) 232
MnlI CCTC 6 cut(s) 158, 199, 331, 453, 465, 619
MseI TTAA 7 cut(s) 39, 216, 243, 333, 540, 561, 755
MslI CAYNNNNRTG 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 215
MspR9I CCNGG 2 cut(s) 567, 790
MvaI CCWGG 2 cut(s) 567, 790
MvnI CGCG 1 cut(s) 546
NdeII GATC 6 cut(s) 287, 343, 628, 644, 649, 706
NlaIII CATG 1 cut(s) 179
NlaIV GGNNCC 1 cut(s) 651
NspI RCATGY 1 cut(s) 179
PfeI GAWTC 4 cut(s) 20, 303, 322, 592
PflMI CCANNNNNTGG 1 cut(s) 467
PkrI GCNGC 2 cut(s) 379, 466
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 565, 788
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 2 cut(s) 565, 788
PspN4I GGNNCC 1 cut(s) 651
PsrI GAACNNNNNNTAC 2 cut(s) 474, 506
PstI CTGCAG 1 cut(s) 403
PstNI CAGNNNCTG 2 cut(s) 407, 467
PsuI RGATCY 2 cut(s) 287, 649
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 48, 441
RsaNI GTAC 2 cut(s) 47, 440
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 7 cut(s) 39, 216, 243, 333, 540, 561, 755
SatI GCNGC 2 cut(s) 378, 465
Sau3AI GATC 6 cut(s) 287, 343, 628, 644, 649, 706
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 2 cut(s) 567, 790
SexAI ACCWGGT 2 cut(s) 565, 788
SfaNI GCATC 5 cut(s) 175, 364, 404, 517, 645
SfcI CTRYAG 3 cut(s) 357, 399, 694
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 215, 350
SmoI CTYRAG 2 cut(s) 215, 350
Sse9I AATT 3 cut(s) 69, 365, 388
SsiI CCGC 2 cut(s) 544, 815
StyD4I CCNGG 2 cut(s) 565, 788
TaaI ACNGT 1 cut(s) 760
TaqI TCGA 2 cut(s) 325, 802
TasI AATT 3 cut(s) 69, 365, 388
TfiI GAWTC 4 cut(s) 20, 303, 322, 592
Tru1I TTAA 7 cut(s) 39, 216, 243, 333, 540, 561, 755
Tru9I TTAA 7 cut(s) 39, 216, 243, 333, 540, 561, 755
TseI GCWGC 2 cut(s) 377, 464
TspGWI ACGGA 1 cut(s) 530
Van91I CCANNNNNTGG 1 cut(s) 467
Vha464I CTTAAG 1 cut(s) 215
XapI RAATTY 1 cut(s) 388
XceI RCATGY 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.