MD10G1243300.v1.1

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011459
Physical Location & Seq
Forward (+)
33792290 .. 33795960
3671 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1243300.v1.1.491

Sequence Viewer

Length: 972 bp
ATGCAGCATCACTCCATTTTATGCTCCCAGCTACTTTCAAAAGACTTCATCTTTGCCGCCGTGCGACGTTGTTTTGCCAAGGAACCGTTTAAAGCACCCAACAGCCGCCGAAACGGAACGGAAACTCGAGTATCAATGGAGAAAGGTGGGTTAGACTCCGCCGGCCGAGAATTCAAGAACGCAGAGGAGATGTGGAGGGAGCAGGTCGGGGACGACCACAGCAAGAAGACCGAGTGGTACCGCCAAGGCGTTGGCTACTGGGAAGGTGTGGAGGCGTCGACCAATGGAGTGTTGGGAGGATATGCGCAGGTGAATGAGCCTGACATAATGGGCAGTGAAGCTTTTCTTAAGCAGCTTCTCTCTGAGCGTTTTCCCGACGCTGCAAATGGCCAACGTCACCTTGTTGTTCTTGATTGTGGTTCTGGCATTGGCAGAGTCTCCAAAAATCTTCTCATAAGATACTTCAATGAGGTTGATCTTCTTGAGCCCGTATCTCATTTCTTGGAAACTGCTCGTGAAAGTTTGGCTCCTGAAAATCATATGATCTCTGATACGCACAAAGCTACTAACTTTTTCTGTGTGCCTCTTCAGGAATTCACACCAGAAGCAGGGAGGTACGACATTATATGGGTTCAATGGTGTATTGGTCATCTGACAGATGATGACTTTGTCTCATTCTTTAAACGGGCAAAGGTTGGATTAAAACCTGGTGGTCTTTTTATTCTGAAGGAAAATATTGCAAGATCCGGATTCGTGTTAGACACAGAAGATCGGAGTGTCACTAGGTCTGATTTGTACTTTAAGGAGCTCTTTCGTCAGTGTGGACTTCATCTTTACATATCAAAGGATCAAAAGGGGTTGCCTGTAGAATTATTCGCCGTGAAAATGTATGCATTAACTACCGAGTTGCCAAAGAAGGTCCATCGGACGAGATCTAAAGTGCAAGCCAACAGACCTGGAGTCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

324

Amino Acids

36.73

Weight (kDa)

8.14

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_PK PF05891 76 - 300 1.4e-92 AdoMet dependent proline di-methyltransferase
Methyltransf_23 PF13489 119 - 244 4.9e-06 Methyltransferase domain
Methyltransf_25 PF13649 136 - 238 1.8e-09 Methyltransferase domain
Methyltransf_11 PF08241 137 - 242 8.9e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
Acc16I TGCGCA 1 cut(s) 306
Acc36I ACCTGC 2 cut(s) 193, 298
Acc65I GGTACC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 278
AccIII TCCGGA 1 cut(s) 746
AciI CCGC 4 cut(s) 57, 106, 159, 241
AclWI GGATC 2 cut(s) 738, 855
AcoI YGGCCR 2 cut(s) 163, 388
AcsI RAATTY 2 cut(s) 170, 593
AcuI CTGAAG 2 cut(s) 572, 746
AcyI GRCGYC 1 cut(s) 275
AfaI GTAC 3 cut(s) 239, 617, 797
AfiI CCNNNNNNNGG 1 cut(s) 608
AflII CTTAAG 1 cut(s) 347
AgsI TTSAA 4 cut(s) 39, 175, 466, 635
AhdI GACNNNNNGTC 1 cut(s) 959
AjnI CCWGG 2 cut(s) 706, 955
AluBI AGCT 5 cut(s) 31, 341, 355, 563, 808
AluI AGCT 5 cut(s) 31, 341, 355, 563, 808
Alw21I GWGCWC 1 cut(s) 810
Alw26I GTCTC 2 cut(s) 442, 676
AlwI GGATC 2 cut(s) 738, 855
Ama87I CYCGRG 1 cut(s) 126
Aor13HI TCCGGA 1 cut(s) 746
AoxI GGCC 2 cut(s) 163, 388
ApeKI GCWGC 3 cut(s) 4, 352, 380
ApoI RAATTY 2 cut(s) 170, 593
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
Asp700I GAANNNNTTC 1 cut(s) 342
Asp718I GGTACC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 1 cut(s) 919
AsuHPI GGTGA 2 cut(s) 322, 389
AvaI CYCGRG 1 cut(s) 126
AvaII GGWCC 1 cut(s) 919
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
BalI TGGCCA 1 cut(s) 390
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 2 cut(s) 489, 810
BauI CACGAG 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 810
BbvI GCAGC 3 cut(s) 16, 364, 367
BccI CCATC 1 cut(s) 930
BceAI ACGGC 2 cut(s) 44, 863
BciT130I CCWGG 2 cut(s) 708, 957
BcoDI GTCTC 2 cut(s) 442, 676
BfaI CTAG 1 cut(s) 783
BfmI CTRYAG 1 cut(s) 864
BfrI CTTAAG 1 cut(s) 347
BfuAI ACCTGC 2 cut(s) 193, 298
BglII AGATCT 1 cut(s) 932
BisI GCNGC 5 cut(s) 5, 57, 106, 353, 381
BlsI GCNGC 5 cut(s) 6, 58, 107, 354, 382
Bme1390I CCNGG 2 cut(s) 708, 957
Bme18I GGWCC 1 cut(s) 919
BmeRI GACNNNNNGTC 1 cut(s) 959
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 919
BmiI GGNNCC 3 cut(s) 84, 239, 528
BmrFI CCNGG 2 cut(s) 708, 957
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 1 cut(s) 16
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 503
BsaHI GRCGYC 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 78, 244
BsaWI WCCGGW 1 cut(s) 746
Bsc4I CCNNNNNNNGG 1 cut(s) 608
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 263
BseAI TCCGGA 1 cut(s) 746
BseBI CCWGG 2 cut(s) 708, 957
BseDI CCNNGG 2 cut(s) 78, 244
BseLI CCNNNNNNNGG 1 cut(s) 608
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 1 cut(s) 200
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 3 cut(s) 16, 364, 367
BseYI CCCAGC 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 2 cut(s) 165, 390
BshNI GGYRCC 1 cut(s) 237
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 810
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 2 cut(s) 162, 747
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 1 cut(s) 608
BsmAI GTCTC 2 cut(s) 442, 676
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 2 cut(s) 165, 390
BsoBI CYCGRG 1 cut(s) 126
Bsp1286I GDGCHC 2 cut(s) 489, 810
Bsp13I TCCGGA 1 cut(s) 746
Bsp143I GATC 6 cut(s) 475, 543, 743, 769, 847, 932
BspACI CCGC 4 cut(s) 57, 106, 159, 241
BspANI GGCC 2 cut(s) 165, 390
BspCNI CTCAG 1 cut(s) 355
BspEI TCCGGA 1 cut(s) 746
BspLI GGNNCC 3 cut(s) 84, 239, 528
BspMI ACCTGC 2 cut(s) 193, 298
BspPI GGATC 2 cut(s) 738, 855
BspT107I GGYRCC 1 cut(s) 237
BspTI CTTAAG 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 78, 244
BssMI GATC 6 cut(s) 475, 543, 743, 769, 847, 932
BssNI GRCGYC 1 cut(s) 275
BssSI CACGAG 1 cut(s) 513
BssT1I CCWWGG 2 cut(s) 78, 244
Bst2BI CACGAG 1 cut(s) 513
Bst2UI CCWGG 2 cut(s) 708, 957
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 591
BstACI GRCGYC 1 cut(s) 275
BstAFI CTTAAG 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 163, 945
BstDEI CTNAG 1 cut(s) 363
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 6 cut(s) 478, 546, 746, 772, 850, 935
BstMAI GTCTC 2 cut(s) 442, 676
BstMBI GATC 6 cut(s) 475, 543, 743, 769, 847, 932
BstMCI CGRYCG 1 cut(s) 166
BstNI CCWGG 2 cut(s) 708, 957
BstSCI CCNGG 2 cut(s) 706, 955
BstSFI CTRYAG 1 cut(s) 864
BstV1I GCAGC 3 cut(s) 16, 364, 367
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 2 cut(s) 743, 932
BstXI CCANNNNNNTGG 1 cut(s) 251
BstYI RGATCY 2 cut(s) 743, 932
BstZI CGGCCG 1 cut(s) 163
BsuRI GGCC 2 cut(s) 165, 390
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 340, 824
BveI ACCTGC 2 cut(s) 193, 298
Cac8I GCNNGC 2 cut(s) 163, 945
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 919
CseI GACGC 2 cut(s) 264, 386
CsiI ACCWGGT 1 cut(s) 706
Csp6I GTAC 3 cut(s) 238, 616, 796
CviQI GTAC 3 cut(s) 238, 616, 796
DdeI CTNAG 1 cut(s) 363
DpnI GATC 6 cut(s) 477, 545, 745, 771, 849, 934
DpnII GATC 6 cut(s) 475, 543, 743, 769, 847, 932
DraI TTTAAA 2 cut(s) 91, 682
DriI GACNNNNNGTC 1 cut(s) 959
EaeI YGGCCR 2 cut(s) 163, 388
EagI CGGCCG 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 591
Eam1105I GACNNNNNGTC 1 cut(s) 959
EarI CTCTTC 1 cut(s) 591
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 808
EclXI CGGCCG 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 78, 244
Eco24I GRGCYC 2 cut(s) 489, 810
Eco47I GGWCC 1 cut(s) 919
Eco52I CGGCCG 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 808
Eco57I CTGAAG 2 cut(s) 572, 746
Eco88I CYCGRG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 808
EcoRI GAATTC 2 cut(s) 170, 593
EcoRII CCWGG 2 cut(s) 706, 955
EcoT14I CCWWGG 2 cut(s) 78, 244
EcoT22I ATGCAT 1 cut(s) 895
EcoT38I GRGCYC 2 cut(s) 489, 810
ErhI CCWWGG 2 cut(s) 78, 244
FalI AAGNNNNNCTT 4 cut(s) 330, 362, 794, 826
FaqI GGGAC 1 cut(s) 224
FauNDI CATATG 1 cut(s) 540
FblI GTMKAC 1 cut(s) 278
Fnu4HI GCNGC 5 cut(s) 5, 57, 106, 353, 381
FriOI GRGCYC 2 cut(s) 489, 810
Fsp4HI GCNGC 5 cut(s) 5, 57, 106, 353, 381
FspBI CTAG 1 cut(s) 783
FspI TGCGCA 1 cut(s) 306
GlaI GCGC 1 cut(s) 306
GluI GCNGC 5 cut(s) 5, 57, 106, 353, 381
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 2 cut(s) 165, 390
HapII CCGG 2 cut(s) 162, 747
HgaI GACGC 2 cut(s) 264, 386
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 275
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HindIII AAGCTT 1 cut(s) 339
HinfI GANTC 4 cut(s) 155, 435, 750, 960
HpaII CCGG 2 cut(s) 162, 747
HphI GGTGA 2 cut(s) 322, 389
Hpy166II GTNNAC 2 cut(s) 279, 824
Hpy188I TCNGA 7 cut(s) 364, 550, 654, 726, 774, 790, 927
Hpy188III TCNNGA 8 cut(s) 175, 374, 410, 482, 515, 530, 590, 747
Hpy8I GTNNAC 2 cut(s) 279, 824
Hpy99I CGWCG 3 cut(s) 69, 280, 380
HpyAV CCTTC 3 cut(s) 257, 721, 910
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 2 cut(s) 67, 394
HpyCH4V TGCA 5 cut(s) 4, 383, 740, 893, 943
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 67, 394
Hsp92I GRCGYC 1 cut(s) 275
HspAI GCGC 1 cut(s) 305
Kpn2I TCCGGA 1 cut(s) 746
KpnI GGTACC 1 cut(s) 241
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 6 cut(s) 475, 543, 743, 769, 847, 932
LmnI GCTCC 4 cut(s) 29, 199, 532, 805
Lsp1109I GCAGC 3 cut(s) 16, 364, 367
LweI GCATC 1 cut(s) 16
MabI ACCWGGT 1 cut(s) 706
MaeI CTAG 1 cut(s) 783
MaeII ACGT 2 cut(s) 67, 394
MaeIII GTNAC 2 cut(s) 395, 778
MalI GATC 6 cut(s) 477, 545, 745, 771, 849, 934
MboI GATC 6 cut(s) 475, 543, 743, 769, 847, 932
MboII GAAGA 5 cut(s) 238, 440, 470, 578, 779
MflI RGATCY 2 cut(s) 743, 932
MhlI GDGCHC 2 cut(s) 489, 810
MlsI TGGCCA 1 cut(s) 390
MluCI AATT 3 cut(s) 170, 593, 869
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 3 cut(s) 149, 444, 969
MmeI TCCRAC 1 cut(s) 676
MnlI CCTC 7 cut(s) 178, 189, 265, 290, 463, 594, 606
Mox20I TGGCCA 1 cut(s) 390
Mph1103I ATGCAT 1 cut(s) 895
MroI TCCGGA 1 cut(s) 746
MroNI GCCGGC 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 342
MscI TGGCCA 1 cut(s) 390
MseI TTAA 6 cut(s) 90, 348, 681, 701, 801, 896
Msp20I TGGCCA 1 cut(s) 390
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 2 cut(s) 162, 747
MspR9I CCNGG 2 cut(s) 708, 957
MvaI CCWGG 2 cut(s) 708, 957
NaeI GCCGGC 1 cut(s) 163
NdeI CATATG 1 cut(s) 540
NdeII GATC 6 cut(s) 475, 543, 743, 769, 847, 932
NgoMIV GCCGGC 1 cut(s) 161
NlaIV GGNNCC 3 cut(s) 84, 239, 528
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 2 cut(s) 395, 778
NsbI TGCGCA 1 cut(s) 306
NsiI ATGCAT 1 cut(s) 895
PaeR7I CTCGAG 1 cut(s) 126
PaqCI CACCTGC 1 cut(s) 298
PdiI GCCGGC 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 750
PflFI GACNNNGTC 1 cut(s) 668
PkrI GCNGC 5 cut(s) 6, 58, 107, 354, 382
PleI GAGTC 3 cut(s) 149, 443, 968
PpsI GAGTC 3 cut(s) 149, 443, 968
Psp124BI GAGCTC 1 cut(s) 810
Psp6I CCWGG 2 cut(s) 706, 955
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 2 cut(s) 706, 955
PspN4I GGNNCC 3 cut(s) 84, 239, 528
PspPI GGNCC 1 cut(s) 919
PspXI VCTCGAGB 1 cut(s) 126
PsuI RGATCY 2 cut(s) 743, 932
PsyI GACNNNGTC 1 cut(s) 668
RsaI GTAC 3 cut(s) 239, 617, 797
RsaNI GTAC 3 cut(s) 238, 616, 796
SacI GAGCTC 1 cut(s) 810
SalI GTCGAC 1 cut(s) 277
SaqAI TTAA 6 cut(s) 90, 348, 681, 701, 801, 896
SatI GCNGC 5 cut(s) 5, 57, 106, 353, 381
Sau3AI GATC 6 cut(s) 475, 543, 743, 769, 847, 932
Sau96I GGNCC 1 cut(s) 919
SchI GAGTC 3 cut(s) 149, 444, 969
ScrFI CCNGG 2 cut(s) 708, 957
SduI GDGCHC 2 cut(s) 489, 810
SexAI ACCWGGT 1 cut(s) 706
SfaNI GCATC 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 864
Sfr274I CTCGAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 919
SlaI CTCGAG 1 cut(s) 126
SmlI CTYRAG 3 cut(s) 126, 347, 482
SmoI CTYRAG 3 cut(s) 126, 347, 482
Sse9I AATT 3 cut(s) 170, 593, 869
SsiI CCGC 4 cut(s) 57, 106, 159, 241
SspI AATATT 1 cut(s) 736
SspMI CTAG 1 cut(s) 783
SstI GAGCTC 1 cut(s) 810
StyD4I CCNGG 2 cut(s) 706, 955
StyI CCWWGG 2 cut(s) 78, 244
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 2 cut(s) 70, 397
TaqI TCGA 2 cut(s) 127, 278
TaqII GACCGA 1 cut(s) 245
TasI AATT 3 cut(s) 170, 593, 869
TatI WGTACW 1 cut(s) 795
TauI GCSGC 2 cut(s) 59, 108
TfiI GAWTC 1 cut(s) 750
Tru1I TTAA 6 cut(s) 90, 348, 681, 701, 801, 896
Tru9I TTAA 6 cut(s) 90, 348, 681, 701, 801, 896
TscAI CASTG 2 cut(s) 340, 824
TseFI GTSAC 2 cut(s) 395, 778
TseI GCWGC 3 cut(s) 4, 352, 380
Tsp45I GTSAC 2 cut(s) 395, 778
TspDTI ATGAA 2 cut(s) 37, 818
TspGWI ACGGA 2 cut(s) 129, 134
TspRI CASTG 2 cut(s) 340, 824
Tth111I GACNNNGTC 1 cut(s) 668
Vha464I CTTAAG 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 919
XapI RAATTY 2 cut(s) 170, 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XhoI CTCGAG 1 cut(s) 126
XmiI GTMKAC 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 1 cut(s) 783
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.