Rw5G011590

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011459
Physical Location & Seq
Forward (+)
13352019 .. 13355758
3740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011590.1

Sequence Viewer

Length: 939 bp
ATGCTATTATACTCAGAGCTACTATTAACAAAAGGCTTGACCTTTTTTGGGGCCAAAGACTTGACCTTTAACAAAACCCAGAAAAACCCGGCTAGGATTTCGATGCAGAAAGGTGGGTTGGACTCCGATGGCCGGGAATTCAAGACCGCCGAGGAGATGTGGAGGGAGCAGGTCGGAGATGAGAGCAAGAAGACTGATTGGTACCGTGAAGGCGTTGGCTACTGGGAAGGTGTGGAGGCTTCGAATGATGGAGTGTTGGGTGGATTTGGGCATGTGAGTGAGACTGACATAAGGGATAGTGAAGCTTTTCTTAATGGGATTATATCTGAGGTTTTGCCCAATGCTGCTAATGGCCAACGCCACCTTGTTGCTCTTGATTGTGGTTCTGGCATTGGGAGGATCACAAAAAATCTTCTCATAAGATACTTCAATGAGGTTGATCTTCTGGAGCCTGTGTCACATTTTTTAGAAACTGCTCGGGAGAGTTTGGCTCCTGAAAATCAGATGGTCTCTGATATGCACAAAGCCACTAATTTTTTCTGCGTGCCTCTTCAGGAATTCACACCAGAAGCAGGAAGATATGATATTATATGGGTTCAGTGGTGTATTGGCCATCTCACAGATGATGACTTTGTGTCATTCTTTACAAGAGCAAAGGTTGGATTAAAACCAGGTGGTTTATTTGTCCTTAAGGAAAATATCGCTAGAACCGGAGGATTTGTGTTAGACAAAGAAGATCGGAGCATCACGAGGTCTGATTCGTACTTTAAGGAGCTCTTCCGTCAGTGTGGACTTCATCTTTACAAAATAAAGGATCAAAAGGGATGGCCTCAGGAATTGTTTGCAGTGAAAATGTATGCATTAACCACCGAATTACCTAAGAAGGTCCATCGGACTAGATCTAAGTTGCAAGCCAATATACCAGCAATCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

312

Amino Acids

35.37

Weight (kDa)

6.21

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_PK PF05891 64 - 289 9e-93 AdoMet dependent proline di-methyltransferase
Methyltransf_25 PF13649 125 - 226 1.4e-08 Methyltransferase domain
Methyltransf_11 PF08241 125 - 230 1.6e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 201
AciI CCGC 1 cut(s) 147
AclWI GGATC 2 cut(s) 407, 822
AcoI YGGCCR 3 cut(s) 130, 352, 610
AcsI RAATTY 2 cut(s) 137, 557
AcuI CTGAAG 1 cut(s) 536
AfaI GTAC 2 cut(s) 203, 764
AfiI CCNNNNNNNGG 3 cut(s) 48, 132, 572
AflII CTTAAG 1 cut(s) 689
AgsI TTSAA 2 cut(s) 142, 430
AhdI GACNNNNNGTC 1 cut(s) 634
AjnI CCWGG 1 cut(s) 670
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AluBI AGCT 3 cut(s) 19, 305, 775
AluI AGCT 3 cut(s) 19, 305, 775
Alw21I GWGCWC 1 cut(s) 777
Alw26I GTCTC 2 cut(s) 275, 514
AlwI GGATC 2 cut(s) 407, 822
Ama87I CYCGRG 1 cut(s) 477
AoxI GGCC 5 cut(s) 51, 130, 352, 610, 827
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 2 cut(s) 137, 557
Asp700I GAANNNNTTC 1 cut(s) 306
Asp718I GGTACC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 51, 886
AsuC2I CCSGG 2 cut(s) 89, 134
AsuII TTCGAA 1 cut(s) 242
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 1 cut(s) 886
AxyI CCTNAGG 1 cut(s) 831
BalI TGGCCA 2 cut(s) 354, 612
BanI GGYRCC 1 cut(s) 201
BanII GRGCYC 1 cut(s) 777
BauI CACGAG 1 cut(s) 748
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 777
BbvI GCAGC 1 cut(s) 331
BccI CCATC 6 cut(s) 122, 242, 499, 621, 819, 897
BciT130I CCWGG 1 cut(s) 672
BcnI CCSGG 2 cut(s) 89, 134
BcoDI GTCTC 2 cut(s) 275, 514
BfaI CTAG 3 cut(s) 93, 705, 897
BfrI CTTAAG 1 cut(s) 689
BfuAI ACCTGC 1 cut(s) 160
BglII AGATCT 1 cut(s) 899
BisI GCNGC 1 cut(s) 345
BlsI GCNGC 1 cut(s) 346
Bme1390I CCNGG 3 cut(s) 89, 134, 672
Bme18I GGWCC 1 cut(s) 886
BmeRI GACNNNNNGTC 1 cut(s) 634
BmeT110I CYCGRG 1 cut(s) 477
BmgT120I GGNCC 2 cut(s) 51, 886
BmiI GGNNCC 4 cut(s) 52, 203, 450, 492
BmrFI CCNGG 3 cut(s) 89, 134, 672
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 2 cut(s) 93, 753
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 197
BplI GAGNNNNNCTC 2 cut(s) 475, 507
BpmI CTGGAG 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 242
BpuMI CCSGG 2 cut(s) 89, 134
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 1 cut(s) 150
BsaWI WCCGGW 1 cut(s) 710
BsaXI ACNNNNNCTCC 4 cut(s) 440, 470, 705, 735
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 132, 572
Bse1I ACTGG 1 cut(s) 227
Bse21I CCTNAGG 1 cut(s) 831
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 830
BseLI CCNNNNNNNGG 3 cut(s) 48, 132, 572
BseMII CTCAG 3 cut(s) 27, 318, 845
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 1 cut(s) 331
BshFI GGCC 5 cut(s) 53, 132, 354, 612, 829
BshNI GGYRCC 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 777
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 3 cut(s) 89, 133, 711
BslI CCNNNNNNNGG 3 cut(s) 48, 132, 572
BsmAI GTCTC 2 cut(s) 275, 514
BsnI GGCC 5 cut(s) 53, 132, 354, 612, 829
Bso31I GGTCTC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 477
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 777
Bsp143I GATC 5 cut(s) 399, 439, 736, 814, 899
BspACI CCGC 1 cut(s) 147
BspANI GGCC 5 cut(s) 53, 132, 354, 612, 829
BspCNI CTCAG 3 cut(s) 26, 319, 844
BspLI GGNNCC 4 cut(s) 52, 203, 450, 492
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 2 cut(s) 407, 822
BspQI GCTCTTC 1 cut(s) 782
BspT104I TTCGAA 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 514
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 5 cut(s) 399, 439, 736, 814, 899
BssSI CACGAG 1 cut(s) 748
Bst2BI CACGAG 1 cut(s) 748
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 1 cut(s) 206
Bst6I CTCTTC 2 cut(s) 555, 782
BstAFI CTTAAG 1 cut(s) 689
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 545, 912
BstDEI CTNAG 5 cut(s) 13, 327, 831, 879, 903
BstF5I GGATG 1 cut(s) 830
BstKTI GATC 5 cut(s) 402, 442, 739, 817, 902
BstMAI GTCTC 2 cut(s) 275, 514
BstMBI GATC 5 cut(s) 399, 439, 736, 814, 899
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 1 cut(s) 275
BstSCI CCNGG 3 cut(s) 87, 132, 670
BstV1I GCAGC 1 cut(s) 331
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 899
BstYI RGATCY 1 cut(s) 899
Bsu36I CCTNAGG 1 cut(s) 831
BsuRI GGCC 5 cut(s) 53, 132, 354, 612, 829
BtsCI GGATG 1 cut(s) 830
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 3 cut(s) 605, 791, 852
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 2 cut(s) 545, 912
Cfr13I GGNCC 2 cut(s) 51, 886
CsiI ACCWGGT 1 cut(s) 670
Csp6I GTAC 2 cut(s) 202, 763
CviAII CATG 1 cut(s) 272
CviQI GTAC 2 cut(s) 202, 763
DdeI CTNAG 5 cut(s) 13, 327, 831, 879, 903
DpnI GATC 5 cut(s) 401, 441, 738, 816, 901
DpnII GATC 5 cut(s) 399, 439, 736, 814, 899
DriI GACNNNNNGTC 1 cut(s) 634
EaeI YGGCCR 3 cut(s) 130, 352, 610
Eam1104I CTCTTC 2 cut(s) 555, 782
Eam1105I GACNNNNNGTC 1 cut(s) 634
EarI CTCTTC 2 cut(s) 555, 782
Ecl136II GAGCTC 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 777
Eco31I GGTCTC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 775
Eco57I CTGAAG 1 cut(s) 536
Eco81I CCTNAGG 1 cut(s) 831
Eco88I CYCGRG 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 775
EcoRI GAATTC 2 cut(s) 137, 557
EcoRII CCWGG 1 cut(s) 670
EcoT22I ATGCAT 1 cut(s) 862
EcoT38I GRGCYC 1 cut(s) 777
FaeI CATG 1 cut(s) 275
FalI AAGNNNNNCTT 4 cut(s) 294, 326, 761, 793
FatI CATG 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 345
FokI GGATG 1 cut(s) 837
FriOI GRGCYC 1 cut(s) 777
Fsp4HI GCNGC 1 cut(s) 345
FspBI CTAG 3 cut(s) 93, 705, 897
GluI GCNGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 467
HaeIII GGCC 5 cut(s) 53, 132, 354, 612, 829
HapII CCGG 3 cut(s) 89, 133, 711
Hin1II CATG 1 cut(s) 275
HindIII AAGCTT 1 cut(s) 303
HinfI GANTC 2 cut(s) 122, 758
HpaII CCGG 3 cut(s) 89, 133, 711
Hpy166II GTNNAC 1 cut(s) 791
Hpy188I TCNGA 9 cut(s) 16, 127, 176, 328, 504, 514, 741, 757, 894
Hpy188III TCNNGA 8 cut(s) 142, 374, 446, 479, 494, 554, 748, 833
Hpy8I GTNNAC 1 cut(s) 791
HpyAV CCTTC 3 cut(s) 203, 221, 877
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4V TGCA 5 cut(s) 106, 520, 845, 860, 910
HpyF3I CTNAG 5 cut(s) 13, 327, 831, 879, 903
Hsp92II CATG 1 cut(s) 275
KpnI GGTACC 1 cut(s) 205
Kzo9I GATC 5 cut(s) 399, 439, 736, 814, 899
LguI GCTCTTC 1 cut(s) 782
LmnI GCTCC 5 cut(s) 166, 448, 496, 741, 772
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 2 cut(s) 93, 753
MabI ACCWGGT 1 cut(s) 670
MaeI CTAG 3 cut(s) 93, 705, 897
MaeIII GTNAC 1 cut(s) 456
MalI GATC 5 cut(s) 401, 441, 738, 816, 901
MboI GATC 5 cut(s) 399, 439, 736, 814, 899
MboII GAAGA 7 cut(s) 202, 404, 434, 542, 588, 746, 769
MflI RGATCY 1 cut(s) 899
MhlI GDGCHC 1 cut(s) 777
MlsI TGGCCA 2 cut(s) 354, 612
MluCI AATT 5 cut(s) 137, 532, 557, 836, 872
MluNI TGGCCA 2 cut(s) 354, 612
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 3 cut(s) 99, 154, 640
Mox20I TGGCCA 2 cut(s) 354, 612
Mph1103I ATGCAT 1 cut(s) 862
MroXI GAANNNNTTC 1 cut(s) 306
MscI TGGCCA 2 cut(s) 354, 612
MseI TTAA 7 cut(s) 26, 69, 312, 665, 690, 768, 863
MslI CAYNNNNRTG 1 cut(s) 276
Msp20I TGGCCA 2 cut(s) 354, 612
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 3 cut(s) 89, 133, 711
MspR9I CCNGG 3 cut(s) 89, 134, 672
MvaI CCWGG 1 cut(s) 672
NciI CCSGG 2 cut(s) 89, 134
NdeII GATC 5 cut(s) 399, 439, 736, 814, 899
NlaIII CATG 1 cut(s) 275
NlaIV GGNNCC 4 cut(s) 52, 203, 450, 492
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 456
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 1 cut(s) 275
NspV TTCGAA 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 782
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 758
PkrI GCNGC 1 cut(s) 346
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 777
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 4 cut(s) 52, 203, 450, 492
PspPI GGNCC 2 cut(s) 51, 886
PsuI RGATCY 1 cut(s) 899
RsaI GTAC 2 cut(s) 203, 764
RsaNI GTAC 2 cut(s) 202, 763
RseI CAYNNNNRTG 1 cut(s) 276
SacI GAGCTC 1 cut(s) 777
SapI GCTCTTC 1 cut(s) 782
SaqAI TTAA 7 cut(s) 26, 69, 312, 665, 690, 768, 863
SatI GCNGC 1 cut(s) 345
Sau3AI GATC 5 cut(s) 399, 439, 736, 814, 899
Sau96I GGNCC 2 cut(s) 51, 886
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 3 cut(s) 89, 134, 672
SduI GDGCHC 1 cut(s) 777
SexAI ACCWGGT 1 cut(s) 670
SfaNI GCATC 2 cut(s) 93, 753
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 1 cut(s) 886
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 5 cut(s) 137, 532, 557, 836, 872
SsiI CCGC 1 cut(s) 147
SspMI CTAG 3 cut(s) 93, 705, 897
SstI GAGCTC 1 cut(s) 777
StyD4I CCNGG 3 cut(s) 87, 132, 670
TaaI ACNGT 1 cut(s) 206
TaqI TCGA 2 cut(s) 101, 242
TasI AATT 5 cut(s) 137, 532, 557, 836, 872
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 7 cut(s) 26, 69, 312, 665, 690, 768, 863
Tru9I TTAA 7 cut(s) 26, 69, 312, 665, 690, 768, 863
TscAI CASTG 3 cut(s) 605, 791, 852
TseFI GTSAC 1 cut(s) 456
TseI GCWGC 1 cut(s) 344
Tsp45I GTSAC 1 cut(s) 456
TspDTI ATGAA 1 cut(s) 785
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 3 cut(s) 605, 791, 852
Vha464I CTTAAG 1 cut(s) 689
VpaK11BI GGWCC 1 cut(s) 886
XapI RAATTY 2 cut(s) 137, 557
XceI RCATGY 1 cut(s) 275
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 3 cut(s) 93, 705, 897
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.