Rroxscaffold_1G00059540

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011459
Physical Location & Seq
Reverse (-)
81537679 .. 81540791
3113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059540.1

Sequence Viewer

Length: 990 bp
ATGCAGAAAGGTGGGTTGGACTCCGACGGCCGGGAATTCAAGACCGCCGAGGAGATGTGGAGGGAGCAGGTCGGAGATGAGAGCAAGAAGACGGATTGGTACCGTGAAGGGGTTGGCTACTGGGAAGGTGTGGAGGCTTCGAATGATGGCGTGTTGGGTGGATTTGGGCATGTGAGTGAGACTGACATAAGGGATAGTGAAGCTTTTCTTAGTGGGATTATCTGTGAGGTTTTGCCCAATGCTGCTAATGGCCAACGCCACCTTGTTGCTCTTGGTATGCAAAGCTCTCTCTTTCTTTATGGTTGTGTGTGTTGGTTTTGTTCAGAAGTGTCTAATGAACTGTGCAAGAACAAATTGTGGTTCTGGCATTGGGAGGATCACAAAAATCTTCTCATAAGATACTTTAATGAGAAACTGCTCGGGAGAGTTTGGCTCCCTGAAAATCAGATGGTCTCTGATATGCACAAAGCCACTAATTTTTTCTGCATGCCTCTTCAGGAATTCACACCACCAGAAGCAGGAAGATATGATATTATATGGGTTCAGTGGTGTATTGGGCATCTCACAGATGATGACTTTGTGTCATTCTTTACAAGAGCAAAGGTAGAGCTGTTTTTCATGAATCTTAATGTGAATGTTGGGTTGAAGAAGAATTTTAACGTAAAACTTTTCCCTTGCACTATGGGAAAAACTGGTATTAGACCAAAAATAAAGAGGAATGGAAAAACTGGTATACTTCTGGCTTCTTCTCAGGCTCAAGCAAGAGGATTCGTGTTAGACAAAGAAGATCGGAGCATCACGAGGTCTGATTCGTACTTTAAGGAGCTCTTCCGTCAGTGTGGACTTCATCTTTACAAAATAAAGGATCAAAAGGGATGGCCTCAGGAATTGTTTGCAGTGAAAATGTATGCATTAACTACCGAATTACCTAAGAAGGTCCATCGGACTAGATCTAAGGTGCAAGCCAATAGACCAGCAATCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

329

Amino Acids

37.99

Weight (kDa)

8.62

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_PK PF05891 30 - 84 2.8e-12 AdoMet dependent proline di-methyltransferase
Methyltransf_PK PF05891 148 - 204 3.2e-21 AdoMet dependent proline di-methyltransferase
Methyltransf_PK PF05891 244 - 306 3.7e-17 AdoMet dependent proline di-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 58
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 733
AciI CCGC 1 cut(s) 45
AclWI GGATC 2 cut(s) 384, 873
AcoI YGGCCR 2 cut(s) 28, 250
AcsI RAATTY 3 cut(s) 35, 500, 652
AcuI CTGAAG 1 cut(s) 479
AfaI GTAC 2 cut(s) 101, 815
AfiI CCNNNNNNNGG 3 cut(s) 30, 109, 518
AgsI TTSAA 2 cut(s) 40, 646
AhdI GACNNNNNGTC 1 cut(s) 580
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 4 cut(s) 203, 285, 610, 826
AluI AGCT 4 cut(s) 203, 285, 610, 826
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 2 cut(s) 173, 457
AlwI GGATC 2 cut(s) 384, 873
Ama87I CYCGRG 1 cut(s) 419
AoxI GGCC 3 cut(s) 28, 250, 878
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 3 cut(s) 35, 500, 652
Asp700I GAANNNNTTC 1 cut(s) 204
Asp718I GGTACC 1 cut(s) 99
AspS9I GGNCC 1 cut(s) 937
AsuC2I CCSGG 1 cut(s) 32
AsuII TTCGAA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 1 cut(s) 937
AxyI CCTNAGG 1 cut(s) 882
BalI TGGCCA 1 cut(s) 252
BanI GGYRCC 1 cut(s) 99
BanII GRGCYC 1 cut(s) 828
BauI CACGAG 1 cut(s) 799
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 828
BbvI GCAGC 1 cut(s) 229
BccI CCATC 4 cut(s) 140, 442, 870, 948
BceAI ACGGC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 173, 457
BfaI CTAG 1 cut(s) 948
BfuAI ACCTGC 1 cut(s) 58
BglII AGATCT 1 cut(s) 950
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 937
BmeRI GACNNNNNGTC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 937
BmiI GGNNCC 2 cut(s) 101, 434
BmrFI CCNGG 1 cut(s) 32
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 2 cut(s) 568, 804
BmuI ACTGGG 1 cut(s) 130
BpiI GAAGAC 1 cut(s) 95
BplI GAGNNNNNCTC 2 cut(s) 417, 449
Bpu14I TTCGAA 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 741
BpuMI CCSGG 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 109, 518
Bse1I ACTGG 3 cut(s) 125, 697, 733
Bse21I CCTNAGG 1 cut(s) 882
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 1 cut(s) 881
BseLI CCNNNNNNNGG 3 cut(s) 30, 109, 518
BseMII CTCAG 2 cut(s) 764, 896
BseNI ACTGG 3 cut(s) 125, 697, 733
BseRI GAGGAG 1 cut(s) 65
BseX3I CGGCCG 1 cut(s) 28
BseXI GCAGC 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 3 cut(s) 30, 252, 880
BshNI GGYRCC 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 419
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 3 cut(s) 30, 109, 518
BsmAI GTCTC 2 cut(s) 173, 457
BsnI GGCC 3 cut(s) 30, 252, 880
Bso31I GGTCTC 1 cut(s) 457
BsoBI CYCGRG 1 cut(s) 419
Bsp119I TTCGAA 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 828
Bsp143I GATC 4 cut(s) 376, 787, 865, 950
BspACI CCGC 1 cut(s) 45
BspANI GGCC 3 cut(s) 30, 252, 880
BspCNI CTCAG 2 cut(s) 763, 895
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 2 cut(s) 101, 434
BspMI ACCTGC 1 cut(s) 58
BspPI GGATC 2 cut(s) 384, 873
BspQI GCTCTTC 1 cut(s) 833
BspT104I TTCGAA 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 457
BsrI ACTGG 3 cut(s) 125, 697, 733
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 4 cut(s) 376, 787, 865, 950
BssNAI GTATAC 1 cut(s) 734
BssSI CACGAG 1 cut(s) 799
Bst1107I GTATAC 1 cut(s) 734
Bst2BI CACGAG 1 cut(s) 799
Bst4CI ACNGT 2 cut(s) 104, 342
Bst6I CTCTTC 2 cut(s) 498, 833
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 488, 963
BstDEI CTNAG 5 cut(s) 209, 750, 882, 930, 954
BstF5I GGATG 1 cut(s) 881
BstKTI GATC 4 cut(s) 379, 790, 868, 953
BstMAI GTCTC 2 cut(s) 173, 457
BstMBI GATC 4 cut(s) 376, 787, 865, 950
BstMCI CGRYCG 1 cut(s) 31
BstNSI RCATGY 2 cut(s) 173, 490
BstSCI CCNGG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 229
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 950
BstYI RGATCY 1 cut(s) 950
BstZ17I GTATAC 1 cut(s) 734
BstZI CGGCCG 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 882
BsuRI GGCC 3 cut(s) 30, 252, 880
BtsCI GGATG 1 cut(s) 881
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 3 cut(s) 551, 842, 903
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 2 cut(s) 488, 963
CciI TCATGA 1 cut(s) 618
Cfr13I GGNCC 1 cut(s) 937
Csp6I GTAC 2 cut(s) 100, 814
CviAII CATG 3 cut(s) 170, 487, 619
CviQI GTAC 2 cut(s) 100, 814
DdeI CTNAG 5 cut(s) 209, 750, 882, 930, 954
DpnI GATC 4 cut(s) 378, 789, 867, 952
DpnII GATC 4 cut(s) 376, 787, 865, 950
DriI GACNNNNNGTC 1 cut(s) 580
EaeI YGGCCR 2 cut(s) 28, 250
EagI CGGCCG 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 498, 833
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 2 cut(s) 498, 833
Ecl136II GAGCTC 1 cut(s) 826
EclXI CGGCCG 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 828
Eco31I GGTCTC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 937
Eco52I CGGCCG 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 1 cut(s) 479
Eco81I CCTNAGG 1 cut(s) 882
Eco88I CYCGRG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 826
EcoRI GAATTC 2 cut(s) 35, 500
EcoT22I ATGCAT 1 cut(s) 913
EcoT38I GRGCYC 1 cut(s) 828
FaeI CATG 3 cut(s) 173, 490, 622
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 812, 844
FatI CATG 3 cut(s) 169, 486, 618
FblI GTMKAC 1 cut(s) 733
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 1 cut(s) 888
FriOI GRGCYC 1 cut(s) 828
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 1 cut(s) 948
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 3 cut(s) 30, 252, 880
HapII CCGG 1 cut(s) 31
Hin1II CATG 3 cut(s) 173, 490, 622
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 4 cut(s) 20, 622, 768, 809
HpaII CCGG 1 cut(s) 31
Hpy166II GTNNAC 2 cut(s) 734, 842
Hpy188I TCNGA 8 cut(s) 25, 74, 325, 447, 457, 792, 808, 945
Hpy188III TCNNGA 6 cut(s) 40, 421, 497, 619, 799, 884
Hpy8I GTNNAC 2 cut(s) 734, 842
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 3 cut(s) 101, 119, 928
HpyCH4III ACNGT 2 cut(s) 104, 342
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 9 cut(s) 4, 280, 345, 463, 486, 678, 896, 911, 961
HpyF3I CTNAG 5 cut(s) 209, 750, 882, 930, 954
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 3 cut(s) 173, 490, 622
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 4 cut(s) 376, 787, 865, 950
LguI GCTCTTC 1 cut(s) 833
LmnI GCTCC 4 cut(s) 64, 438, 792, 823
Lsp1109I GCAGC 1 cut(s) 229
LweI GCATC 2 cut(s) 568, 804
MaeI CTAG 1 cut(s) 948
MaeII ACGT 1 cut(s) 660
MalI GATC 4 cut(s) 378, 789, 867, 952
MboI GATC 4 cut(s) 376, 787, 865, 950
MboII GAAGA 9 cut(s) 100, 380, 485, 534, 658, 661, 738, 797, 820
MflI RGATCY 1 cut(s) 950
MhlI GDGCHC 1 cut(s) 828
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 7 cut(s) 35, 353, 475, 500, 652, 887, 923
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 48, 52
Mox20I TGGCCA 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 913
MroXI GAANNNNTTC 1 cut(s) 204
MscI TGGCCA 1 cut(s) 252
MseI TTAA 5 cut(s) 405, 627, 657, 819, 914
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 252
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 1 cut(s) 32
NciI CCSGG 1 cut(s) 32
NdeII GATC 4 cut(s) 376, 787, 865, 950
NlaIII CATG 3 cut(s) 173, 490, 622
NlaIV GGNNCC 2 cut(s) 101, 434
NmeAIII GCCGAG 1 cut(s) 73
NsiI ATGCAT 1 cut(s) 913
NspI RCATGY 2 cut(s) 173, 490
NspV TTCGAA 1 cut(s) 140
PaeI GCATGC 1 cut(s) 490
PagI TCATGA 1 cut(s) 618
PciSI GCTCTTC 1 cut(s) 833
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 3 cut(s) 622, 768, 809
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 828
PspN4I GGNNCC 2 cut(s) 101, 434
PspPI GGNCC 1 cut(s) 937
PsuI RGATCY 1 cut(s) 950
RsaI GTAC 2 cut(s) 101, 815
RsaNI GTAC 2 cut(s) 100, 814
RseI CAYNNNNRTG 1 cut(s) 174
SacI GAGCTC 1 cut(s) 828
SapI GCTCTTC 1 cut(s) 833
SaqAI TTAA 5 cut(s) 405, 627, 657, 819, 914
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 4 cut(s) 376, 787, 865, 950
Sau96I GGNCC 1 cut(s) 937
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 1 cut(s) 828
SfaNI GCATC 2 cut(s) 568, 804
SfuI TTCGAA 1 cut(s) 140
SinI GGWCC 1 cut(s) 937
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 756
SmoI CTYRAG 1 cut(s) 756
SphI GCATGC 1 cut(s) 490
Sse9I AATT 7 cut(s) 35, 353, 475, 500, 652, 887, 923
SsiI CCGC 1 cut(s) 45
SspMI CTAG 1 cut(s) 948
SstI GAGCTC 1 cut(s) 828
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 104, 342
TaiI ACGT 1 cut(s) 663
TaqI TCGA 1 cut(s) 140
TasI AATT 7 cut(s) 35, 353, 475, 500, 652, 887, 923
TfiI GAWTC 3 cut(s) 622, 768, 809
Tru1I TTAA 5 cut(s) 405, 627, 657, 819, 914
Tru9I TTAA 5 cut(s) 405, 627, 657, 819, 914
TscAI CASTG 3 cut(s) 551, 842, 903
TseI GCWGC 1 cut(s) 242
TspDTI ATGAA 4 cut(s) 351, 607, 635, 836
TspGWI ACGGA 2 cut(s) 107, 821
TspRI CASTG 3 cut(s) 551, 842, 903
VpaK11BI GGWCC 1 cut(s) 937
XapI RAATTY 3 cut(s) 35, 500, 652
XceI RCATGY 2 cut(s) 173, 490
XmiI GTMKAC 1 cut(s) 733
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 1 cut(s) 948
Zsp2I ATGCAT 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.