Rh5CG143600

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011459
Physical Location & Seq
Forward (+)
12770784 .. 12773644
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG143600.1

Sequence Viewer

Length: 837 bp
ATGCAGAAAGGTGGGTTGGACTCCGATGGCCGGGAATTCAAGACCGCCGAGGAGATGTGGAGGGAGCAGGTCGGAGATGAGAGCAAGAAGACGGATTGGTACCGGGAAGGGGTTGGCTACTGGGAAGGTGTGGAGGCTTCGAATGATGGAGTGTTGGGTGGATTTGGGCATGTGAGTGAGACTGACATAAGGGATAGTGAAGCTTTTCTTAATGGGATTATATCTGAGGTTTTGCCCAGTGCTGCTAATGGCCAACGCCACCTTGTTGCTCTTGATTGTGGTTCTGGCATTGGGAGGATCACAAGAAATCTTCTCATAAGATACTTCAATGAGGTTGATCTTCTGGAGCCTGTGTCACATTTTTTAGAAACTGCTCGGGAGAGTTTGGCTCCTGAAAATCAGATGGTCTCTGATATGCACAAAGCCACTAATTTTTTCTGCGTGCCTCTTCAGGAATTCACACCAGAAGCAGGAAGATATGATATTATATGGGTTCAGTGGTGTATTGGGCATCTCACAGATGATGACTTTGTGTCATTCTTTACAAGAGCAAAGGTTGGATTAAAACCAGGTGGTTTATTTGTCCTTAAGGAAAATATCGCTAGAACTGGAGGATTTGTGTTAGACAAAGAAGATCGGAGCATCACGAGGTCTGATTCGTACTTTAAGGAGCTCTTCCGTCAGTGTGGACTTCATCTTTACAAAATAAAGGATCAAAAGGGATGGCCTCAGGAATTGTTTGCAGTGAAAATGTATGCATTAACTACCGAATTACCTAAGAAGGTCCATCGGACTAGATCTAAGGTGCAAGCCAATAGACCAGCAATCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

278

Amino Acids

31.56

Weight (kDa)

5.87

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_PK PF05891 30 - 255 2.4e-92 AdoMet dependent proline di-methyltransferase
Methyltransf_25 PF13649 91 - 192 7.7e-09 Methyltransferase domain
Methyltransf_11 PF08241 91 - 196 1.3e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 58
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AciI CCGC 1 cut(s) 45
AclWI GGATC 2 cut(s) 305, 720
AcoI YGGCCR 2 cut(s) 28, 250
AcsI RAATTY 2 cut(s) 35, 455
AcuI CTGAAG 1 cut(s) 434
AfaI GTAC 2 cut(s) 101, 662
AfiI CCNNNNNNNGG 3 cut(s) 30, 109, 470
AflII CTTAAG 1 cut(s) 587
AgsI TTSAA 2 cut(s) 40, 328
AhdI GACNNNNNGTC 1 cut(s) 532
AjnI CCWGG 1 cut(s) 568
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 2 cut(s) 203, 673
AluI AGCT 2 cut(s) 203, 673
Alw21I GWGCWC 1 cut(s) 675
Alw26I GTCTC 2 cut(s) 173, 412
AlwI GGATC 2 cut(s) 305, 720
Ama87I CYCGRG 1 cut(s) 375
AoxI GGCC 3 cut(s) 28, 250, 725
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 2 cut(s) 35, 455
Asp700I GAANNNNTTC 1 cut(s) 204
Asp718I GGTACC 1 cut(s) 99
AspS9I GGNCC 1 cut(s) 784
AsuC2I CCSGG 2 cut(s) 32, 104
AsuII TTCGAA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 375
AvaII GGWCC 1 cut(s) 784
AxyI CCTNAGG 1 cut(s) 729
BalI TGGCCA 1 cut(s) 252
BanI GGYRCC 1 cut(s) 99
BanII GRGCYC 1 cut(s) 675
BauI CACGAG 1 cut(s) 646
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 675
BbvI GCAGC 1 cut(s) 229
BccI CCATC 5 cut(s) 20, 140, 397, 717, 795
BciT130I CCWGG 1 cut(s) 570
BcnI CCSGG 2 cut(s) 32, 104
BcoDI GTCTC 2 cut(s) 173, 412
BfaI CTAG 2 cut(s) 603, 795
BfrI CTTAAG 1 cut(s) 587
BfuAI ACCTGC 1 cut(s) 58
BglII AGATCT 1 cut(s) 797
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 3 cut(s) 32, 104, 570
Bme18I GGWCC 1 cut(s) 784
BmeRI GACNNNNNGTC 1 cut(s) 532
BmeT110I CYCGRG 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 784
BmiI GGNNCC 3 cut(s) 101, 348, 390
BmrFI CCNGG 3 cut(s) 32, 104, 570
BmrI ACTGGG 2 cut(s) 130, 231
BmsI GCATC 2 cut(s) 520, 651
BmuI ACTGGG 2 cut(s) 130, 231
BpiI GAAGAC 1 cut(s) 95
BplI GAGNNNNNCTC 2 cut(s) 373, 405
BpmI CTGGAG 2 cut(s) 365, 630
Bpu14I TTCGAA 1 cut(s) 140
BpuMI CCSGG 2 cut(s) 32, 104
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 48
BsaXI ACNNNNNCTCC 4 cut(s) 338, 368, 603, 633
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 109, 470
Bse1I ACTGG 3 cut(s) 125, 237, 613
Bse21I CCTNAGG 1 cut(s) 729
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 1 cut(s) 728
BseLI CCNNNNNNNGG 3 cut(s) 30, 109, 470
BseMII CTCAG 2 cut(s) 216, 743
BseNI ACTGG 3 cut(s) 125, 237, 613
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 229
BshFI GGCC 3 cut(s) 30, 252, 727
BshNI GGYRCC 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 675
BsiHKCI CYCGRG 1 cut(s) 375
BsiSI CCGG 2 cut(s) 31, 103
BslI CCNNNNNNNGG 3 cut(s) 30, 109, 470
BsmAI GTCTC 2 cut(s) 173, 412
BsnI GGCC 3 cut(s) 30, 252, 727
Bso31I GGTCTC 1 cut(s) 412
BsoBI CYCGRG 1 cut(s) 375
Bsp119I TTCGAA 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 675
Bsp143I GATC 5 cut(s) 297, 337, 634, 712, 797
BspACI CCGC 1 cut(s) 45
BspANI GGCC 3 cut(s) 30, 252, 727
BspCNI CTCAG 2 cut(s) 217, 742
BspLI GGNNCC 3 cut(s) 101, 348, 390
BspMI ACCTGC 1 cut(s) 58
BspPI GGATC 2 cut(s) 305, 720
BspQI GCTCTTC 1 cut(s) 680
BspT104I TTCGAA 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 99
BspTI CTTAAG 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 412
BsrI ACTGG 3 cut(s) 125, 237, 613
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 5 cut(s) 297, 337, 634, 712, 797
BssSI CACGAG 1 cut(s) 646
Bst2BI CACGAG 1 cut(s) 646
Bst2UI CCWGG 1 cut(s) 570
Bst6I CTCTTC 2 cut(s) 453, 680
BstAFI CTTAAG 1 cut(s) 587
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 443, 810
BstDEI CTNAG 4 cut(s) 225, 729, 777, 801
BstF5I GGATG 1 cut(s) 728
BstKTI GATC 5 cut(s) 300, 340, 637, 715, 800
BstMAI GTCTC 2 cut(s) 173, 412
BstMBI GATC 5 cut(s) 297, 337, 634, 712, 797
BstNI CCWGG 1 cut(s) 570
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 3 cut(s) 30, 102, 568
BstV1I GCAGC 1 cut(s) 229
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
Bsu36I CCTNAGG 1 cut(s) 729
BsuRI GGCC 3 cut(s) 30, 252, 727
BtsCI GGATG 1 cut(s) 728
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 4 cut(s) 244, 503, 689, 750
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 2 cut(s) 443, 810
Cfr13I GGNCC 1 cut(s) 784
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 2 cut(s) 100, 661
CviAII CATG 1 cut(s) 170
CviQI GTAC 2 cut(s) 100, 661
DdeI CTNAG 4 cut(s) 225, 729, 777, 801
DpnI GATC 5 cut(s) 299, 339, 636, 714, 799
DpnII GATC 5 cut(s) 297, 337, 634, 712, 797
DriI GACNNNNNGTC 1 cut(s) 532
EaeI YGGCCR 2 cut(s) 28, 250
Eam1104I CTCTTC 2 cut(s) 453, 680
Eam1105I GACNNNNNGTC 1 cut(s) 532
EarI CTCTTC 2 cut(s) 453, 680
Ecl136II GAGCTC 1 cut(s) 673
Eco24I GRGCYC 1 cut(s) 675
Eco31I GGTCTC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 784
Eco53kI GAGCTC 1 cut(s) 673
Eco57I CTGAAG 1 cut(s) 434
Eco81I CCTNAGG 1 cut(s) 729
Eco88I CYCGRG 1 cut(s) 375
EcoICRI GAGCTC 1 cut(s) 673
EcoRI GAATTC 2 cut(s) 35, 455
EcoRII CCWGG 1 cut(s) 568
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 1 cut(s) 675
FaeI CATG 1 cut(s) 173
FaiI YATR 9 cut(s) 171, 188, 221, 317, 416, 480, 488, 490, 756
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 659, 691
FatI CATG 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 1 cut(s) 735
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 2 cut(s) 603, 795
GluI GCNGC 1 cut(s) 243
GsuI CTGGAG 2 cut(s) 365, 630
HaeIII GGCC 3 cut(s) 30, 252, 727
HapII CCGG 2 cut(s) 31, 103
Hin1II CATG 1 cut(s) 173
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 2 cut(s) 20, 656
HpaII CCGG 2 cut(s) 31, 103
Hpy166II GTNNAC 1 cut(s) 689
Hpy188I TCNGA 8 cut(s) 25, 74, 226, 402, 412, 639, 655, 792
Hpy188III TCNNGA 8 cut(s) 40, 272, 344, 377, 392, 452, 646, 731
Hpy8I GTNNAC 1 cut(s) 689
HpyAV CCTTC 3 cut(s) 101, 119, 775
HpyCH4V TGCA 5 cut(s) 4, 418, 743, 758, 808
HpyF3I CTNAG 4 cut(s) 225, 729, 777, 801
Hsp92II CATG 1 cut(s) 173
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 5 cut(s) 297, 337, 634, 712, 797
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 5 cut(s) 64, 346, 394, 639, 670
Lsp1109I GCAGC 1 cut(s) 229
LweI GCATC 2 cut(s) 520, 651
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 2 cut(s) 603, 795
MaeIII GTNAC 1 cut(s) 354
MalI GATC 5 cut(s) 299, 339, 636, 714, 799
MboI GATC 5 cut(s) 297, 337, 634, 712, 797
MboII GAAGA 7 cut(s) 100, 302, 332, 440, 486, 644, 667
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 675
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 5 cut(s) 35, 430, 455, 734, 770
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 52, 538
Mox20I TGGCCA 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 760
MroXI GAANNNNTTC 1 cut(s) 204
MscI TGGCCA 1 cut(s) 252
MseI TTAA 5 cut(s) 210, 563, 588, 666, 761
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 252
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 2 cut(s) 31, 103
MspR9I CCNGG 3 cut(s) 32, 104, 570
MvaI CCWGG 1 cut(s) 570
NciI CCSGG 2 cut(s) 32, 104
NdeII GATC 5 cut(s) 297, 337, 634, 712, 797
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 3 cut(s) 101, 348, 390
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 1 cut(s) 354
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 1 cut(s) 173
NspV TTCGAA 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 680
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 1 cut(s) 656
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 3 cut(s) 101, 348, 390
PspPI GGNCC 1 cut(s) 784
PsuI RGATCY 1 cut(s) 797
RsaI GTAC 2 cut(s) 101, 662
RsaNI GTAC 2 cut(s) 100, 661
RseI CAYNNNNRTG 1 cut(s) 174
SacI GAGCTC 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 680
SaqAI TTAA 5 cut(s) 210, 563, 588, 666, 761
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 5 cut(s) 297, 337, 634, 712, 797
Sau96I GGNCC 1 cut(s) 784
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 3 cut(s) 32, 104, 570
SduI GDGCHC 1 cut(s) 675
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 2 cut(s) 520, 651
SfuI TTCGAA 1 cut(s) 140
SinI GGWCC 1 cut(s) 784
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 5 cut(s) 35, 430, 455, 734, 770
SsiI CCGC 1 cut(s) 45
SspMI CTAG 2 cut(s) 603, 795
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 3 cut(s) 30, 102, 568
TaqI TCGA 1 cut(s) 140
TasI AATT 5 cut(s) 35, 430, 455, 734, 770
TfiI GAWTC 1 cut(s) 656
Tru1I TTAA 5 cut(s) 210, 563, 588, 666, 761
Tru9I TTAA 5 cut(s) 210, 563, 588, 666, 761
TscAI CASTG 4 cut(s) 244, 503, 689, 750
TseFI GTSAC 1 cut(s) 354
TseI GCWGC 1 cut(s) 242
Tsp45I GTSAC 1 cut(s) 354
TspDTI ATGAA 1 cut(s) 683
TspGWI ACGGA 2 cut(s) 107, 668
TspRI CASTG 4 cut(s) 244, 503, 689, 750
Vha464I CTTAAG 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 784
XapI RAATTY 2 cut(s) 35, 455
XceI RCATGY 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 2 cut(s) 603, 795
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.