MD05G1265300.v1.1

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011459
Physical Location & Seq
Forward (+)
40031050 .. 40036157
5108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1265300.v1.1.491

Sequence Viewer

Length: 960 bp
ATGATCCGGTCCCAAGTTACTTTCAATAGACTTCCATCTTTGCCGCTGTGCGACGTCGTTTTTAGCCAAGACCCGTTTAACACACCCAACCGCCGCCGAAACAGAACCGAAACTCGAGTGTCAATGGAGAAAGGCGGGTTAGACTTCGCCGGCCGAGAATTTAAGAACGCGGAGGAGATGTGGAGGGAGCAGCTCGGAGAAGACCACAGCAAGAAGACCGAGTGGTACCGCCAAGGCGTTGGCTACTGGGAAGGTGTGGAGGCGTCGACGAATGGAGTTTTGGGAGGATATGCGCAGGTGAATGAGCCTGACATAATGGGCAGTGAAGCTTTTCTTAAGCAGCTTCTTTCTGAGCGTTTTCCTGGTGCTACAAATGGCCAACGCCACCTTGTTGTTCTTGATTGTGGTTCTGGCATTGGGAGAGTCTCCAAAAATCTTCTCATAAGATACTTCAATGAGGTTGATCTACTTGAGCCTGTATCACATTTCTTGGAAACTGCTCGTGAAAGTTTGGCTCCTGAAAATCATAAGGTCTCTGATATGCACAAAGCTACTAATTTTTTCTGCATGCCTCTTCAGGAATTCACACCAGACGCAGGGAGGTACAACATTATATGGGTTCAATGGTGTATTGGGCATCTGACAGATGACGACTTCGTCTCATTCTTTAAACGGGCAAAGGTAGGATTAAAACCTGGTGGTCTTTTTGTCCTAAAGGAAAATATTGTAAGATCCGGATTTGTGTTAGACACAGAAGACCGGAGTGTCACCAGGTCGGATTTGTACTTTAGGGAGCTCTTTCGTCAGTGTGGACTTCATCTTTACATATCAAAGGATCAAAAGGGGTTGCCTGAGGAATTATTTGCTGTGAAAATGTATGCATTAACTACTGAGTTGCCAAAGAAGGTCCATCGGACAAGATCTGAAGTGCAAGCCAATAGACCCGGAGTCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

320

Amino Acids

36.56

Weight (kDa)

7.68

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_PK PF05891 72 - 296 3.4e-92 AdoMet dependent proline di-methyltransferase
Methyltransf_25 PF13649 132 - 234 7.7e-09 Methyltransferase domain
Methyltransf_11 PF08241 133 - 238 1.7e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
AasI GACNNNNNNGTC 2 cut(s) 656, 764
AatII GACGTC 1 cut(s) 57
Acc16I TGCGCA 1 cut(s) 294
Acc36I ACCTGC 1 cut(s) 286
Acc65I GGTACC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 266
AccII CGCG 1 cut(s) 170
AccIII TCCGGA 1 cut(s) 734
AciI CCGC 6 cut(s) 44, 91, 94, 135, 170, 229
AclWI GGATC 2 cut(s) 726, 843
AcoI YGGCCR 2 cut(s) 151, 376
AcsI RAATTY 2 cut(s) 158, 581
AcuI CTGAAG 2 cut(s) 560, 945
AcyI GRCGYC 2 cut(s) 54, 263
AfaI GTAC 3 cut(s) 227, 605, 785
AfiI CCNNNNNNNGG 1 cut(s) 596
AflII CTTAAG 1 cut(s) 335
AgsI TTSAA 3 cut(s) 25, 454, 623
AhdI GACNNNNNGTC 1 cut(s) 947
AjnI CCWGG 3 cut(s) 361, 694, 770
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 5 cut(s) 193, 329, 343, 551, 796
AluI AGCT 5 cut(s) 193, 329, 343, 551, 796
Alw21I GWGCWC 1 cut(s) 798
Alw26I GTCTC 3 cut(s) 430, 538, 664
AlwI GGATC 2 cut(s) 726, 843
Ama87I CYCGRG 1 cut(s) 114
Aor13HI TCCGGA 1 cut(s) 734
AoxI GGCC 2 cut(s) 151, 376
ApeKI GCWGC 2 cut(s) 190, 340
ApoI RAATTY 2 cut(s) 158, 581
Asp700I GAANNNNTTC 1 cut(s) 330
Asp718I GGTACC 1 cut(s) 225
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 2 cut(s) 9, 907
AsuC2I CCSGG 1 cut(s) 945
AsuHPI GGTGA 2 cut(s) 310, 760
AvaI CYCGRG 1 cut(s) 114
AvaII GGWCC 2 cut(s) 9, 907
AxyI CCTNAGG 1 cut(s) 852
BalI TGGCCA 1 cut(s) 378
BanI GGYRCC 1 cut(s) 225
BanII GRGCYC 1 cut(s) 798
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 3 cut(s) 207, 221, 762
Bbv12I GWGCWC 1 cut(s) 798
BbvI GCAGC 2 cut(s) 202, 352
BccI CCATC 2 cut(s) 43, 918
BciT130I CCWGG 3 cut(s) 363, 696, 772
BcnI CCSGG 1 cut(s) 945
BcoDI GTCTC 3 cut(s) 430, 538, 664
BfrI CTTAAG 1 cut(s) 335
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 920
BisI GCNGC 4 cut(s) 44, 94, 191, 341
BlsI GCNGC 4 cut(s) 45, 95, 192, 342
Bme1390I CCNGG 4 cut(s) 363, 696, 772, 945
Bme18I GGWCC 2 cut(s) 9, 907
BmeRI GACNNNNNGTC 1 cut(s) 947
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 9, 907
BmiI GGNNCC 3 cut(s) 11, 227, 516
BmrFI CCNGG 4 cut(s) 363, 696, 772, 945
BmrI ACTGGG 1 cut(s) 256
BmsI GCATC 1 cut(s) 646
BmuI ACTGGG 1 cut(s) 256
BpiI GAAGAC 3 cut(s) 207, 221, 762
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 945
BsaHI GRCGYC 2 cut(s) 54, 263
BsaI GGTCTC 1 cut(s) 538
BsaJI CCNNGG 1 cut(s) 232
BsaWI WCCGGW 3 cut(s) 6, 734, 759
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 251
Bse21I CCTNAGG 1 cut(s) 852
BseAI TCCGGA 1 cut(s) 734
BseBI CCWGG 3 cut(s) 363, 696, 772
BseDI CCNNGG 1 cut(s) 232
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMII CTCAG 3 cut(s) 342, 843, 882
BseNI ACTGG 1 cut(s) 251
BseRI GAGGAG 1 cut(s) 188
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 202, 352
Bsh1236I CGCG 1 cut(s) 170
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 153, 378
BshNI GGYRCC 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 798
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 5 cut(s) 7, 150, 735, 760, 945
BslI CCNNNNNNNGG 1 cut(s) 596
BsmAI GTCTC 3 cut(s) 430, 538, 664
BsmBI CGTCTC 1 cut(s) 664
BsnI GGCC 2 cut(s) 153, 378
Bso31I GGTCTC 1 cut(s) 538
BsoBI CYCGRG 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 798
Bsp13I TCCGGA 1 cut(s) 734
Bsp143I GATC 5 cut(s) 3, 463, 731, 835, 920
BspACI CCGC 6 cut(s) 44, 91, 94, 135, 170, 229
BspANI GGCC 2 cut(s) 153, 378
BspCNI CTCAG 3 cut(s) 343, 844, 883
BspEI TCCGGA 1 cut(s) 734
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 3 cut(s) 11, 227, 516
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 2 cut(s) 726, 843
BspT107I GGYRCC 1 cut(s) 225
BspTI CTTAAG 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 5 cut(s) 3, 463, 731, 835, 920
BssNI GRCGYC 2 cut(s) 54, 263
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 3 cut(s) 363, 696, 772
Bst6I CTCTTC 1 cut(s) 579
BstACI GRCGYC 2 cut(s) 54, 263
BstAFI CTTAAG 1 cut(s) 335
BstC8I GCNNGC 3 cut(s) 151, 569, 933
BstDEI CTNAG 3 cut(s) 351, 852, 891
BstFNI CGCG 1 cut(s) 170
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 5 cut(s) 6, 466, 734, 838, 923
BstMAI GTCTC 3 cut(s) 430, 538, 664
BstMBI GATC 5 cut(s) 3, 463, 731, 835, 920
BstMCI CGRYCG 1 cut(s) 154
BstNI CCWGG 3 cut(s) 363, 696, 772
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 4 cut(s) 361, 694, 770, 943
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 2 cut(s) 202, 352
BstV2I GAAGAC 3 cut(s) 207, 221, 762
BstX2I RGATCY 2 cut(s) 731, 920
BstXI CCANNNNNNTGG 1 cut(s) 239
BstYI RGATCY 2 cut(s) 731, 920
BstZI CGGCCG 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 852
BsuRI GGCC 2 cut(s) 153, 378
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 2 cut(s) 328, 812
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 151, 569, 933
CfoI GCGC 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 9, 907
CseI GACGC 2 cut(s) 252, 602
CsiI ACCWGGT 2 cut(s) 694, 770
Csp6I GTAC 3 cut(s) 226, 604, 784
CviAII CATG 1 cut(s) 568
CviQI GTAC 3 cut(s) 226, 604, 784
DdeI CTNAG 3 cut(s) 351, 852, 891
DpnI GATC 5 cut(s) 5, 465, 733, 837, 922
DpnII GATC 5 cut(s) 3, 463, 731, 835, 920
DraI TTTAAA 1 cut(s) 670
DrdI GACNNNNNNGTC 2 cut(s) 656, 764
DriI GACNNNNNGTC 1 cut(s) 947
DseDI GACNNNNNNGTC 2 cut(s) 656, 764
EaeI YGGCCR 2 cut(s) 151, 376
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 579
Eam1105I GACNNNNNGTC 1 cut(s) 947
EarI CTCTTC 1 cut(s) 579
Ecl136II GAGCTC 1 cut(s) 796
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 798
Eco31I GGTCTC 1 cut(s) 538
Eco47I GGWCC 2 cut(s) 9, 907
Eco52I CGGCCG 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 796
Eco57I CTGAAG 2 cut(s) 560, 945
Eco81I CCTNAGG 1 cut(s) 852
Eco88I CYCGRG 1 cut(s) 114
EcoICRI GAGCTC 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 3 cut(s) 361, 694, 770
EcoT14I CCWWGG 1 cut(s) 232
EcoT22I ATGCAT 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 798
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 664
FaeI CATG 1 cut(s) 571
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FatI CATG 1 cut(s) 567
FauI CCCGC 1 cut(s) 128
FblI GTMKAC 1 cut(s) 266
Fnu4HI GCNGC 4 cut(s) 44, 94, 191, 341
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 4 cut(s) 44, 94, 191, 341
FspI TGCGCA 1 cut(s) 294
GlaI GCGC 1 cut(s) 294
GluI GCNGC 4 cut(s) 44, 94, 191, 341
HaeIII GGCC 2 cut(s) 153, 378
HapII CCGG 5 cut(s) 7, 150, 735, 760, 945
HgaI GACGC 2 cut(s) 252, 602
HhaI GCGC 1 cut(s) 295
Hin1I GRCGYC 2 cut(s) 54, 263
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 2 cut(s) 423, 948
HpaII CCGG 5 cut(s) 7, 150, 735, 760, 945
HphI GGTGA 2 cut(s) 310, 760
Hpy166II GTNNAC 2 cut(s) 267, 812
Hpy188I TCNGA 7 cut(s) 197, 352, 538, 642, 778, 915, 925
Hpy188III TCNNGA 5 cut(s) 398, 503, 518, 578, 735
Hpy8I GTNNAC 2 cut(s) 267, 812
Hpy99I CGWCG 4 cut(s) 56, 59, 268, 271
HpyAV CCTTC 2 cut(s) 245, 898
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 4 cut(s) 544, 567, 881, 931
HpyF3I CTNAG 3 cut(s) 351, 852, 891
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 2 cut(s) 54, 263
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 1 cut(s) 293
Kpn2I TCCGGA 1 cut(s) 734
KpnI GGTACC 1 cut(s) 229
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Kzo9I GATC 5 cut(s) 3, 463, 731, 835, 920
LmnI GCTCC 3 cut(s) 187, 520, 793
Lsp1109I GCAGC 2 cut(s) 202, 352
LweI GCATC 1 cut(s) 646
MabI ACCWGGT 2 cut(s) 694, 770
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 2 cut(s) 16, 766
MalI GATC 5 cut(s) 5, 465, 733, 837, 922
MboI GATC 5 cut(s) 3, 463, 731, 835, 920
MboII GAAGA 5 cut(s) 212, 226, 428, 566, 767
MflI RGATCY 2 cut(s) 731, 920
MhlI GDGCHC 1 cut(s) 798
MlsI TGGCCA 1 cut(s) 378
MluCI AATT 4 cut(s) 158, 556, 581, 857
MluNI TGGCCA 1 cut(s) 378
MlyI GAGTC 2 cut(s) 432, 957
MmeI TCCRAC 1 cut(s) 756
MnlI CCTC 8 cut(s) 166, 177, 253, 278, 451, 582, 594, 847
Mox20I TGGCCA 1 cut(s) 378
Mph1103I ATGCAT 1 cut(s) 883
MroI TCCGGA 1 cut(s) 734
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 330
MscI TGGCCA 1 cut(s) 378
MseI TTAA 6 cut(s) 78, 162, 336, 669, 689, 884
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 46
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 5 cut(s) 7, 150, 735, 760, 945
MspR9I CCNGG 4 cut(s) 363, 696, 772, 945
MvaI CCWGG 3 cut(s) 363, 696, 772
MvnI CGCG 1 cut(s) 170
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 945
NdeII GATC 5 cut(s) 3, 463, 731, 835, 920
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 3 cut(s) 11, 227, 516
NmeAIII GCCGAG 1 cut(s) 179
NmuCI GTSAC 1 cut(s) 766
NsbI TGCGCA 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 883
NspI RCATGY 1 cut(s) 571
PaeI GCATGC 1 cut(s) 571
PaeR7I CTCGAG 1 cut(s) 114
PaqCI CACCTGC 1 cut(s) 286
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 330
PflFI GACNNNGTC 1 cut(s) 656
PkrI GCNGC 4 cut(s) 45, 95, 192, 342
PleI GAGTC 2 cut(s) 431, 956
PpsI GAGTC 2 cut(s) 431, 956
Psp124BI GAGCTC 1 cut(s) 798
Psp6I CCWGG 3 cut(s) 361, 694, 770
PspGI CCWGG 3 cut(s) 361, 694, 770
PspN4I GGNNCC 3 cut(s) 11, 227, 516
PspPI GGNCC 2 cut(s) 9, 907
PspXI VCTCGAGB 1 cut(s) 114
PsuI RGATCY 2 cut(s) 731, 920
PsyI GACNNNGTC 1 cut(s) 656
RsaI GTAC 3 cut(s) 227, 605, 785
RsaNI GTAC 3 cut(s) 226, 604, 784
SacI GAGCTC 1 cut(s) 798
SalI GTCGAC 1 cut(s) 265
SaqAI TTAA 6 cut(s) 78, 162, 336, 669, 689, 884
SatI GCNGC 4 cut(s) 44, 94, 191, 341
Sau3AI GATC 5 cut(s) 3, 463, 731, 835, 920
Sau96I GGNCC 2 cut(s) 9, 907
SchI GAGTC 2 cut(s) 432, 957
ScrFI CCNGG 4 cut(s) 363, 696, 772, 945
SduI GDGCHC 1 cut(s) 798
SexAI ACCWGGT 2 cut(s) 694, 770
SfaNI GCATC 1 cut(s) 646
Sfr274I CTCGAG 1 cut(s) 114
SgrDI CGTCGACG 1 cut(s) 265
SinI GGWCC 2 cut(s) 9, 907
SlaI CTCGAG 1 cut(s) 114
SmlI CTYRAG 3 cut(s) 114, 335, 470
SmoI CTYRAG 3 cut(s) 114, 335, 470
SphI GCATGC 1 cut(s) 571
Sse9I AATT 4 cut(s) 158, 556, 581, 857
SsiI CCGC 6 cut(s) 44, 91, 94, 135, 170, 229
SspI AATATT 1 cut(s) 724
SstI GAGCTC 1 cut(s) 798
StyD4I CCNGG 4 cut(s) 361, 694, 770, 943
StyI CCWWGG 1 cut(s) 232
TaiI ACGT 1 cut(s) 57
TaqI TCGA 2 cut(s) 115, 266
TaqII GACCGA 1 cut(s) 233
TasI AATT 4 cut(s) 158, 556, 581, 857
TatI WGTACW 1 cut(s) 783
TauI GCSGC 2 cut(s) 46, 96
Tru1I TTAA 6 cut(s) 78, 162, 336, 669, 689, 884
Tru9I TTAA 6 cut(s) 78, 162, 336, 669, 689, 884
TscAI CASTG 2 cut(s) 328, 812
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 2 cut(s) 190, 340
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 1 cut(s) 806
TspRI CASTG 2 cut(s) 328, 812
Tth111I GACNNNGTC 1 cut(s) 656
Vha464I CTTAAG 1 cut(s) 335
VpaK11BI GGWCC 2 cut(s) 9, 907
XapI RAATTY 2 cut(s) 158, 581
XceI RCATGY 1 cut(s) 571
XhoI CTCGAG 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 330
ZraI GACGTC 1 cut(s) 55
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.