Prupe.4G100600_v2.0.a1

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0011459
Physical Location & Seq
Reverse (-)
5131561 .. 5140113
8553 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G100600.1

Sequence Viewer

Length: 969 bp
ATGCAGCATCACTCCATTTTATACTCCGAGCTAATTGCAAAAGACTTCAGCTTTGCCTCTGTGCGACGTCGTTTGGCCAAAGAACCGTTTGACAGAGCCAACAGCCGCCAAAACCAGACCAGAACTCGGGTCTCAATGGAGAAAGGTGGGTTAGACTCTGCCGGCCGAGAATTCAAGAACGCAAAGGAGATGTGGAGAGAGCAGGTCGGAGAAGACCAGAGCAAGAAGACTGAATGGTACCGTCAAGGCGTTGGCTACTGGGAAGGTGTGGAGGCCTCGGCGAATGGAGTGTTGGGAGGATATGGACATGTCAATGAGCCTGACATAATGGGCAGTGAAGCTTTTCTTAATACGCTTCTCTCTGAGCGTTTTCCTGATGCTGCTAATGGCCAACGCCACCTAGTTGTTCTTGATTGTGGTTCTGGCATTGGGAGAGTCACAAAAAATCTTCTCATAAAATACTTCAATGAGGTTGATCTTCTTGAGCCTGTATCACATTTCTTGGAAACTGCTCGTGAAAGTTTGGCTCCTGAAAATCATATGGCCTCTGATATGCACAAAGCCACTAATTTTTTATGCGTGCCTCTTCAGGAATTCACACCAGAAGCAGGAAGGTATGATATTATATGGGTTCAGTGGTGTATTGGGCATCTGACAGATGATGACTTCGTGTCATTCTTTAAAAGGGCAAAGGTTGGATTAAAACCTGGTGGTCTTTTTGTCCTGAAGGAAAATATTGCAAGAACCGGATTCGTATTAGACACTGAAGATCGGAGCATCACCAGGTCTGATTTGTACTTTAAGGAGCTCTTTTGCCAGTGTGGACTTCATATTTACAAATCTAAGGATCAAAAGGGATTGCCTGAGGAGTTATTCGCTGTGAAAATGTATGCATTAACTACTGACTTGCCAAAGGTCCATCGGACCAAATCTAAAGTGCAAGCCAATAGACCCGGTGTCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

323

Amino Acids

36.6

Weight (kDa)

7.68

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 70
Acc36I ACCTGC 1 cut(s) 193
Acc65I GGTACC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 237
AciI CCGC 1 cut(s) 106
AclWI GGATC 1 cut(s) 855
AcoI YGGCCR 3 cut(s) 75, 163, 388
AcsI RAATTY 2 cut(s) 170, 593
AcuI CTGAAG 4 cut(s) 31, 572, 746, 786
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 2 cut(s) 239, 797
AfiI CCNNNNNNNGG 2 cut(s) 126, 608
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 2 cut(s) 175, 466
AhdI GACNNNNNGTC 2 cut(s) 670, 956
AjnI CCWGG 2 cut(s) 706, 782
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 4 cut(s) 31, 51, 341, 808
AluI AGCT 4 cut(s) 31, 51, 341, 808
Alw21I GWGCWC 1 cut(s) 810
Alw26I GTCTC 1 cut(s) 136
AlwI GGATC 1 cut(s) 855
Ama87I CYCGRG 1 cut(s) 126
AoxI GGCC 5 cut(s) 75, 163, 273, 388, 543
ApeKI GCWGC 2 cut(s) 4, 380
ApoI RAATTY 2 cut(s) 170, 593
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
Asp700I GAANNNNTTC 1 cut(s) 342
Asp718I GGTACC 1 cut(s) 237
AspS9I GGNCC 2 cut(s) 916, 924
AsuC2I CCSGG 1 cut(s) 954
AsuHPI GGTGA 1 cut(s) 772
AvaI CYCGRG 1 cut(s) 126
AvaII GGWCC 2 cut(s) 916, 924
AxyI CCTNAGG 1 cut(s) 864
BalI TGGCCA 2 cut(s) 77, 390
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 810
BauI CACGAG 1 cut(s) 513
BbsI GAAGAC 2 cut(s) 219, 233
Bbv12I GWGCWC 1 cut(s) 810
BbvI GCAGC 2 cut(s) 16, 367
BccI CCATC 1 cut(s) 927
BcgI CGANNNNNNTGC 2 cut(s) 17, 51
BciT130I CCWGG 2 cut(s) 708, 784
BcnI CCSGG 1 cut(s) 954
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 1 cut(s) 401
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 3 cut(s) 5, 106, 381
BlsI GCNGC 3 cut(s) 6, 107, 382
Bme1390I CCNGG 3 cut(s) 708, 784, 954
Bme18I GGWCC 2 cut(s) 916, 924
BmeRI GACNNNNNGTC 2 cut(s) 670, 956
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 2 cut(s) 916, 924
BmiI GGNNCC 2 cut(s) 239, 528
BmrFI CCNGG 3 cut(s) 708, 784, 954
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 4 cut(s) 16, 367, 658, 786
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 2 cut(s) 219, 233
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 954
BsaHI GRCGYC 1 cut(s) 67
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 746
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 608
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 2 cut(s) 263, 817
Bse21I CCTNAGG 1 cut(s) 864
BseBI CCWGG 2 cut(s) 708, 784
BseDI CCNNGG 1 cut(s) 276
BseLI CCNNNNNNNGG 2 cut(s) 126, 608
BseMII CTCAG 2 cut(s) 354, 855
BseNI ACTGG 2 cut(s) 263, 817
BseRI GAGGAG 1 cut(s) 881
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 2 cut(s) 16, 367
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 5 cut(s) 77, 165, 275, 390, 545
BshNI GGYRCC 1 cut(s) 237
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 810
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 3 cut(s) 162, 747, 954
BslI CCNNNNNNNGG 2 cut(s) 126, 608
BsmAI GTCTC 1 cut(s) 136
BsnI GGCC 5 cut(s) 77, 165, 275, 390, 545
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 810
Bsp143I GATC 3 cut(s) 475, 769, 847
BspACI CCGC 1 cut(s) 106
BspANI GGCC 5 cut(s) 77, 165, 275, 390, 545
BspCNI CTCAG 2 cut(s) 355, 856
BspLI GGNNCC 2 cut(s) 239, 528
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 855
BspT107I GGYRCC 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 2 cut(s) 263, 817
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 3 cut(s) 475, 769, 847
BssNI GRCGYC 1 cut(s) 67
BssSI CACGAG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 513
Bst2UI CCWGG 2 cut(s) 708, 784
Bst4CI ACNGT 2 cut(s) 87, 242
Bst6I CTCTTC 1 cut(s) 591
BstACI GRCGYC 1 cut(s) 67
BstC8I GCNNGC 3 cut(s) 163, 581, 942
BstDEI CTNAG 3 cut(s) 363, 843, 864
BstKTI GATC 3 cut(s) 478, 772, 850
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 3 cut(s) 475, 769, 847
BstMCI CGRYCG 1 cut(s) 166
BstNI CCWGG 2 cut(s) 708, 784
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 3 cut(s) 706, 782, 952
BstV1I GCAGC 2 cut(s) 16, 367
BstV2I GAAGAC 2 cut(s) 219, 233
BstZI CGGCCG 1 cut(s) 163
Bsu36I CCTNAGG 1 cut(s) 864
BsuRI GGCC 5 cut(s) 77, 165, 275, 390, 545
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 4 cut(s) 340, 641, 762, 824
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 3 cut(s) 163, 581, 942
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 916, 924
CsiI ACCWGGT 2 cut(s) 706, 782
Csp6I GTAC 2 cut(s) 238, 796
CviAII CATG 1 cut(s) 308
CviQI GTAC 2 cut(s) 238, 796
DdeI CTNAG 3 cut(s) 363, 843, 864
DpnI GATC 3 cut(s) 477, 771, 849
DpnII GATC 3 cut(s) 475, 769, 847
DraI TTTAAA 1 cut(s) 682
DriI GACNNNNNGTC 2 cut(s) 670, 956
EaeI YGGCCR 3 cut(s) 75, 163, 388
EagI CGGCCG 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 591
Eam1105I GACNNNNNGTC 2 cut(s) 670, 956
EarI CTCTTC 1 cut(s) 591
Ecl136II GAGCTC 1 cut(s) 808
EclXI CGGCCG 1 cut(s) 163
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 2 cut(s) 916, 924
Eco52I CGGCCG 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 808
Eco57I CTGAAG 4 cut(s) 31, 572, 746, 786
Eco81I CCTNAGG 1 cut(s) 864
Eco88I CYCGRG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 808
EcoRI GAATTC 2 cut(s) 170, 593
EcoRII CCWGG 2 cut(s) 706, 782
EcoT22I ATGCAT 1 cut(s) 895
EcoT38I GRGCYC 1 cut(s) 810
FaeI CATG 1 cut(s) 311
FalI AAGNNNNNCTT 4 cut(s) 330, 362, 794, 826
FatI CATG 1 cut(s) 307
FauNDI CATATG 1 cut(s) 540
Fnu4HI GCNGC 3 cut(s) 5, 106, 381
FriOI GRGCYC 1 cut(s) 810
Fsp4HI GCNGC 3 cut(s) 5, 106, 381
FspBI CTAG 1 cut(s) 401
GluI GCNGC 3 cut(s) 5, 106, 381
HaeIII GGCC 5 cut(s) 77, 165, 275, 390, 545
HapII CCGG 3 cut(s) 162, 747, 954
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 1 cut(s) 311
HindIII AAGCTT 1 cut(s) 339
HinfI GANTC 3 cut(s) 155, 435, 750
HpaII CCGG 3 cut(s) 162, 747, 954
HphI GGTGA 1 cut(s) 772
Hpy166II GTNNAC 1 cut(s) 824
Hpy188I TCNGA 8 cut(s) 28, 209, 364, 550, 654, 774, 790, 924
Hpy188III TCNNGA 8 cut(s) 175, 374, 410, 482, 515, 530, 590, 724
Hpy8I GTNNAC 1 cut(s) 824
Hpy99I CGWCG 2 cut(s) 69, 72
HpyAV CCTTC 3 cut(s) 257, 606, 721
HpyCH4III ACNGT 2 cut(s) 87, 242
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 6 cut(s) 4, 38, 556, 740, 893, 940
HpyF3I CTNAG 3 cut(s) 363, 843, 864
HpySE526I ACGT 1 cut(s) 67
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 1 cut(s) 311
KpnI GGTACC 1 cut(s) 241
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 3 cut(s) 475, 769, 847
LmnI GCTCC 3 cut(s) 532, 774, 805
Lsp1109I GCAGC 2 cut(s) 16, 367
LweI GCATC 4 cut(s) 16, 367, 658, 786
MabI ACCWGGT 2 cut(s) 706, 782
MaeI CTAG 1 cut(s) 401
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 1 cut(s) 436
MalI GATC 3 cut(s) 477, 771, 849
MboI GATC 3 cut(s) 475, 769, 847
MboII GAAGA 6 cut(s) 224, 238, 440, 470, 578, 779
MhlI GDGCHC 1 cut(s) 810
MlsI TGGCCA 2 cut(s) 77, 390
MluCI AATT 4 cut(s) 33, 170, 568, 593
MluNI TGGCCA 2 cut(s) 77, 390
MlyI GAGTC 2 cut(s) 149, 444
MmeI TCCRAC 2 cut(s) 187, 676
MnlI CCTC 8 cut(s) 67, 265, 286, 290, 463, 556, 594, 859
Mox20I TGGCCA 2 cut(s) 77, 390
Mph1103I ATGCAT 1 cut(s) 895
MroNI GCCGGC 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 342
MscI TGGCCA 2 cut(s) 77, 390
MseI TTAA 5 cut(s) 348, 681, 701, 801, 896
MslI CAYNNNNRTG 1 cut(s) 312
Msp20I TGGCCA 2 cut(s) 77, 390
MspI CCGG 3 cut(s) 162, 747, 954
MspR9I CCNGG 3 cut(s) 708, 784, 954
MvaI CCWGG 2 cut(s) 708, 784
NaeI GCCGGC 1 cut(s) 163
NciI CCSGG 1 cut(s) 954
NdeI CATATG 1 cut(s) 540
NdeII GATC 3 cut(s) 475, 769, 847
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 1 cut(s) 311
NlaIV GGNNCC 2 cut(s) 239, 528
NmeAIII GCCGAG 2 cut(s) 191, 257
NmuCI GTSAC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 895
NspI RCATGY 1 cut(s) 311
PceI AGGCCT 1 cut(s) 275
PciI ACATGT 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 750
PkrI GCNGC 3 cut(s) 6, 107, 382
PleI GAGTC 2 cut(s) 149, 443
PpsI GAGTC 2 cut(s) 149, 443
PscI ACATGT 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 810
Psp6I CCWGG 2 cut(s) 706, 782
PspGI CCWGG 2 cut(s) 706, 782
PspN4I GGNNCC 2 cut(s) 239, 528
PspPI GGNCC 2 cut(s) 916, 924
RsaI GTAC 2 cut(s) 239, 797
RsaNI GTAC 2 cut(s) 238, 796
RseI CAYNNNNRTG 1 cut(s) 312
SacI GAGCTC 1 cut(s) 810
SaqAI TTAA 5 cut(s) 348, 681, 701, 801, 896
SatI GCNGC 3 cut(s) 5, 106, 381
Sau3AI GATC 3 cut(s) 475, 769, 847
Sau96I GGNCC 2 cut(s) 916, 924
SchI GAGTC 2 cut(s) 149, 444
ScrFI CCNGG 3 cut(s) 708, 784, 954
SduI GDGCHC 1 cut(s) 810
SexAI ACCWGGT 2 cut(s) 706, 782
SfaNI GCATC 4 cut(s) 16, 367, 658, 786
SinI GGWCC 2 cut(s) 916, 924
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 4 cut(s) 33, 170, 568, 593
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 1 cut(s) 106
SspI AATATT 1 cut(s) 736
SspMI CTAG 1 cut(s) 401
SstI GAGCTC 1 cut(s) 810
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 706, 782, 952
TaaI ACNGT 2 cut(s) 87, 242
TaiI ACGT 1 cut(s) 70
TasI AATT 4 cut(s) 33, 170, 568, 593
TatI WGTACW 1 cut(s) 795
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 1 cut(s) 750
Tru1I TTAA 5 cut(s) 348, 681, 701, 801, 896
Tru9I TTAA 5 cut(s) 348, 681, 701, 801, 896
TscAI CASTG 4 cut(s) 340, 641, 769, 824
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 2 cut(s) 4, 380
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 1 cut(s) 818
TspRI CASTG 4 cut(s) 340, 641, 769, 824
VpaK11BI GGWCC 2 cut(s) 916, 924
XapI RAATTY 2 cut(s) 170, 593
XceI RCATGY 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 1 cut(s) 401
ZraI GACGTC 1 cut(s) 68
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.