FvH4_3g11170

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011459
Physical Location & Seq
Forward (+)
6582631 .. 6585218
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11170.t2

Sequence Viewer

Length: 957 bp
ATGTTAGCAATAAAAGGCTTCACCTTTCTTGGGTTTGGCTCCAAAGACTTGAACTTTAGCAGAACCCACAAAACCCATTTCAGCTTCAACAAAACCCAGAAAAACCCAGGTCGGATTTCGATGGAGAAAGGTGGGTTGGACTCCGACGGCCGGGAGTTCAAGACAGCGGAGGAGATGTGGAGGGAGGAGGTGGGAGACGAGAGCAAGAAGACAGAGTGGTACAAGGAAGGGGTTGGGTACTGGGAAGGTGTGGAGGCTTCAACTGATGGAGTGTTGGGTGGGTTTGGGCATGTGAGTGAGGCTGACATAAGAGATAGTGAAGCTTTTCTTAATGGGCTTCTCTCTGAGGTTCTGCCCAATGCTGCTAATGGCCAACGTCACCTTGTTGCTCTTGATTGTGGTTCTGGCATTGGGAGGATCACAAAGAATCTGCTCATAAGGTACTTCAATGAGGTTGATCTTCTGGAGCCTGTGACACATTTCTTAGAAACTGCTCGCGAGAGTTTGGCTCCTGAGAATCTGATGGTTTCTGATATGCACAAAGCTACTAATTTTTTCTGCGTGCCTCTTCAGGAATTCACCCCGGAAGCAGGAAGATATGATATTATATGGGTTCAGTGGTGTATTGGGCATCTCACAGATGACGACTTTGTGTCATTCTTTACAAGAGCAAAGGTTGGATTAAAACCAGGTGGTTTATTTGTCCTCAAGGAAAATATTGCTAGAACCGGAGGATTCGTGTTAGACAAAGAAGATCGGAGCATCACGAGGTCTGATTTGTACTTTAAGGAGCTCTTCCGCCAGTGTGGACTTCATCTTCACAGAATAAAGGATCAAAATGGATGGCCTAAGGAATTGTTTGCAGTGAAAATGTATGCATTAACTACTGATTTACCTAAGAAGGTCCATCGGACAAGATCTAAGGTGCAAGCCAATAGACCAGCAATCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

319

Amino Acids

36.04

Weight (kDa)

6.81

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_PK PF05891 30 - 188 4.3e-62 AdoMet dependent proline di-methyltransferase
Methyltransf_25 PF13649 91 - 184 1.7e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 498
AciI CCGC 2 cut(s) 167, 799
AclWI GGATC 2 cut(s) 425, 840
AcoI YGGCCR 2 cut(s) 148, 370
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 4 cut(s) 221, 239, 443, 782
AfiI CCNNNNNNNGG 3 cut(s) 30, 150, 590
AgsI TTSAA 5 cut(s) 52, 88, 160, 261, 448
AhdI GACNNNNNGTC 1 cut(s) 652
AjnI CCWGG 2 cut(s) 106, 688
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 4 cut(s) 84, 323, 545, 793
AluI AGCT 4 cut(s) 84, 323, 545, 793
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 1 cut(s) 189
AlwI GGATC 2 cut(s) 425, 840
AoxI GGCC 3 cut(s) 148, 370, 845
ApeKI GCWGC 1 cut(s) 362
ApoI RAATTY 1 cut(s) 575
Asp700I GAANNNNTTC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 904
AsuC2I CCSGG 2 cut(s) 152, 584
AsuHPI GGTGA 3 cut(s) 13, 371, 571
AvaII GGWCC 1 cut(s) 904
AxyI CCTNAGG 1 cut(s) 849
BalI TGGCCA 1 cut(s) 372
BanII GRGCYC 1 cut(s) 795
BauI CACGAG 1 cut(s) 766
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 1 cut(s) 349
BccI CCATC 5 cut(s) 115, 260, 517, 837, 915
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 2 cut(s) 108, 690
BcnI CCSGG 2 cut(s) 152, 584
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 1 cut(s) 723
BglII AGATCT 1 cut(s) 917
BisI GCNGC 1 cut(s) 363
BlsI GCNGC 1 cut(s) 364
Bme1390I CCNGG 4 cut(s) 108, 152, 584, 690
Bme18I GGWCC 1 cut(s) 904
BmeRI GACNNNNNGTC 1 cut(s) 652
BmgT120I GGNCC 1 cut(s) 904
BmiI GGNNCC 3 cut(s) 40, 468, 510
BmrFI CCNGG 4 cut(s) 108, 152, 584, 690
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 640, 771
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 493, 525
BpmI CTGGAG 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 692
BpuMI CCSGG 2 cut(s) 152, 584
BsaJI CCNNGG 2 cut(s) 106, 582
BsaWI WCCGGW 1 cut(s) 728
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 723, 753
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 150, 590
Bse1I ACTGG 2 cut(s) 245, 802
Bse21I CCTNAGG 1 cut(s) 849
BseBI CCWGG 2 cut(s) 108, 690
BseDI CCNNGG 2 cut(s) 106, 582
BseGI GGATG 1 cut(s) 848
BseLI CCNNNNNNNGG 3 cut(s) 30, 150, 590
BseMII CTCAG 2 cut(s) 336, 504
BseNI ACTGG 2 cut(s) 245, 802
BseRI GAGGAG 2 cut(s) 185, 200
BseX3I CGGCCG 1 cut(s) 148
BseXI GCAGC 1 cut(s) 349
Bsh1236I CGCG 1 cut(s) 498
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 3 cut(s) 150, 372, 847
BsiEI CGRYCG 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 795
BsiSI CCGG 3 cut(s) 151, 584, 729
BslI CCNNNNNNNGG 3 cut(s) 30, 150, 590
BsmAI GTCTC 1 cut(s) 189
BsmBI CGTCTC 1 cut(s) 189
BsnI GGCC 3 cut(s) 150, 372, 847
Bsp1286I GDGCHC 1 cut(s) 795
Bsp143I GATC 5 cut(s) 417, 457, 754, 832, 917
Bsp68I TCGCGA 1 cut(s) 498
BspACI CCGC 2 cut(s) 167, 799
BspANI GGCC 3 cut(s) 150, 372, 847
BspCNI CTCAG 2 cut(s) 337, 505
BspFNI CGCG 1 cut(s) 498
BspLI GGNNCC 3 cut(s) 40, 468, 510
BspPI GGATC 2 cut(s) 425, 840
BspQI GCTCTTC 1 cut(s) 800
BsrI ACTGG 2 cut(s) 245, 802
BssECI CCNNGG 2 cut(s) 106, 582
BssMI GATC 5 cut(s) 417, 457, 754, 832, 917
BssSI CACGAG 1 cut(s) 766
Bst2BI CACGAG 1 cut(s) 766
Bst2UI CCWGG 2 cut(s) 108, 690
Bst6I CTCTTC 2 cut(s) 573, 800
BstC8I GCNNGC 3 cut(s) 496, 563, 930
BstDEI CTNAG 6 cut(s) 345, 484, 513, 849, 897, 921
BstF5I GGATG 1 cut(s) 848
BstFNI CGCG 1 cut(s) 498
BstKTI GATC 5 cut(s) 420, 460, 757, 835, 920
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 5 cut(s) 417, 457, 754, 832, 917
BstMCI CGRYCG 1 cut(s) 151
BstNI CCWGG 2 cut(s) 108, 690
BstNSI RCATGY 1 cut(s) 293
BstSCI CCNGG 4 cut(s) 106, 150, 582, 688
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 1 cut(s) 349
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 917
BstYI RGATCY 1 cut(s) 917
BstZI CGGCCG 1 cut(s) 148
Bsu36I CCTNAGG 1 cut(s) 849
BsuRI GGCC 3 cut(s) 150, 372, 847
BtsCI GGATG 1 cut(s) 848
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 3 cut(s) 623, 809, 870
BtuMI TCGCGA 1 cut(s) 498
Cac8I GCNNGC 3 cut(s) 496, 563, 930
Cfr13I GGNCC 1 cut(s) 904
CsiI ACCWGGT 1 cut(s) 688
Csp6I GTAC 4 cut(s) 220, 238, 442, 781
CviAII CATG 1 cut(s) 290
CviQI GTAC 4 cut(s) 220, 238, 442, 781
DdeI CTNAG 6 cut(s) 345, 484, 513, 849, 897, 921
DpnI GATC 5 cut(s) 419, 459, 756, 834, 919
DpnII GATC 5 cut(s) 417, 457, 754, 832, 917
DriI GACNNNNNGTC 1 cut(s) 652
EaeI YGGCCR 2 cut(s) 148, 370
EagI CGGCCG 1 cut(s) 148
Eam1104I CTCTTC 2 cut(s) 573, 800
Eam1105I GACNNNNNGTC 1 cut(s) 652
EarI CTCTTC 2 cut(s) 573, 800
EciI GGCGGA 1 cut(s) 788
Ecl136II GAGCTC 1 cut(s) 793
EclXI CGGCCG 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 795
Eco47I GGWCC 1 cut(s) 904
Eco52I CGGCCG 1 cut(s) 148
Eco53kI GAGCTC 1 cut(s) 793
Eco57I CTGAAG 1 cut(s) 554
Eco81I CCTNAGG 1 cut(s) 849
EcoICRI GAGCTC 1 cut(s) 793
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 2 cut(s) 106, 688
EcoT22I ATGCAT 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 795
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 1 cut(s) 293
FaiI YATR 8 cut(s) 291, 308, 437, 536, 600, 608, 610, 876
FalI AAGNNNNNCTT 4 cut(s) 312, 344, 779, 811
FatI CATG 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 363
FokI GGATG 1 cut(s) 855
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 1 cut(s) 363
FspBI CTAG 1 cut(s) 723
GluI GCNGC 1 cut(s) 363
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 3 cut(s) 150, 372, 847
HapII CCGG 3 cut(s) 151, 584, 729
Hin1II CATG 1 cut(s) 293
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 4 cut(s) 140, 427, 517, 735
HpaII CCGG 3 cut(s) 151, 584, 729
HphI GGTGA 3 cut(s) 13, 371, 571
Hpy166II GTNNAC 1 cut(s) 809
Hpy188I TCNGA 8 cut(s) 114, 145, 346, 522, 532, 759, 775, 912
Hpy188III TCNNGA 7 cut(s) 160, 392, 464, 497, 512, 572, 766
Hpy8I GTNNAC 1 cut(s) 809
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 221, 239, 895
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 4 cut(s) 538, 863, 878, 928
HpyF3I CTNAG 6 cut(s) 345, 484, 513, 849, 897, 921
HpySE526I ACGT 1 cut(s) 376
Hsp92II CATG 1 cut(s) 293
Kzo9I GATC 5 cut(s) 417, 457, 754, 832, 917
LguI GCTCTTC 1 cut(s) 800
LmnI GCTCC 5 cut(s) 44, 466, 514, 759, 790
Lsp1109I GCAGC 1 cut(s) 349
LweI GCATC 2 cut(s) 640, 771
MabI ACCWGGT 1 cut(s) 688
MaeI CTAG 1 cut(s) 723
MaeII ACGT 1 cut(s) 376
MaeIII GTNAC 2 cut(s) 377, 472
MalI GATC 5 cut(s) 419, 459, 756, 834, 919
MboI GATC 5 cut(s) 417, 457, 754, 832, 917
MboII GAAGA 7 cut(s) 220, 452, 560, 606, 764, 787, 809
MflI RGATCY 1 cut(s) 917
MhlI GDGCHC 1 cut(s) 795
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 3 cut(s) 550, 575, 854
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 4 cut(s) 92, 117, 168, 658
Mox20I TGGCCA 1 cut(s) 372
Mph1103I ATGCAT 1 cut(s) 880
MroXI GAANNNNTTC 1 cut(s) 324
MscI TGGCCA 1 cut(s) 372
MseI TTAA 4 cut(s) 330, 683, 786, 881
MslI CAYNNNNRTG 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 3 cut(s) 151, 584, 729
MspR9I CCNGG 4 cut(s) 108, 152, 584, 690
MvaI CCWGG 2 cut(s) 108, 690
MvnI CGCG 1 cut(s) 498
NciI CCSGG 2 cut(s) 152, 584
NdeII GATC 5 cut(s) 417, 457, 754, 832, 917
NlaIII CATG 1 cut(s) 293
NlaIV GGNNCC 3 cut(s) 40, 468, 510
NmuCI GTSAC 2 cut(s) 377, 472
NruI TCGCGA 1 cut(s) 498
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 800
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 3 cut(s) 427, 517, 735
PkrI GCNGC 1 cut(s) 364
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 795
Psp6I CCWGG 2 cut(s) 106, 688
PspGI CCWGG 2 cut(s) 106, 688
PspN4I GGNNCC 3 cut(s) 40, 468, 510
PspPI GGNCC 1 cut(s) 904
PsuI RGATCY 1 cut(s) 917
RruI TCGCGA 1 cut(s) 498
RsaI GTAC 4 cut(s) 221, 239, 443, 782
RsaNI GTAC 4 cut(s) 220, 238, 442, 781
RseI CAYNNNNRTG 1 cut(s) 294
SacI GAGCTC 1 cut(s) 795
SapI GCTCTTC 1 cut(s) 800
SaqAI TTAA 4 cut(s) 330, 683, 786, 881
SatI GCNGC 1 cut(s) 363
Sau3AI GATC 5 cut(s) 417, 457, 754, 832, 917
Sau96I GGNCC 1 cut(s) 904
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 4 cut(s) 108, 152, 584, 690
SduI GDGCHC 1 cut(s) 795
SexAI ACCWGGT 1 cut(s) 688
SfaNI GCATC 2 cut(s) 640, 771
SinI GGWCC 1 cut(s) 904
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
Sse9I AATT 3 cut(s) 550, 575, 854
SsiI CCGC 2 cut(s) 167, 799
SspI AATATT 1 cut(s) 718
SspMI CTAG 1 cut(s) 723
SstI GAGCTC 1 cut(s) 795
StyD4I CCNGG 4 cut(s) 106, 150, 582, 688
TaiI ACGT 1 cut(s) 379
TaqI TCGA 1 cut(s) 119
TasI AATT 3 cut(s) 550, 575, 854
TatI WGTACW 1 cut(s) 780
TfiI GAWTC 3 cut(s) 427, 517, 735
Tru1I TTAA 4 cut(s) 330, 683, 786, 881
Tru9I TTAA 4 cut(s) 330, 683, 786, 881
TscAI CASTG 3 cut(s) 623, 809, 870
TseFI GTSAC 2 cut(s) 377, 472
TseI GCWGC 1 cut(s) 362
Tsp45I GTSAC 2 cut(s) 377, 472
TspDTI ATGAA 1 cut(s) 803
TspRI CASTG 3 cut(s) 623, 809, 870
VpaK11BI GGWCC 1 cut(s) 904
XapI RAATTY 1 cut(s) 575
XceI RCATGY 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 1 cut(s) 723
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.