Rorug05G0040300

Alpha N-terminal protein methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011459
Physical Location & Seq
Forward (+)
3486788 .. 3489485
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0040300.1

Sequence Viewer

Length: 810 bp
ATGGGGGATTTAGAGAAGCAAGCAGAGAGGGTCGTGCTGAGCAAGGACGAGGCGGAAGAGGAGGAGATGGAGAGGCTGGTCGGCGGCGGCGGCGGAATGGCGGTGTTGGATTTCGACATGCTGTGCTCCACCGTCGCCTTACAGACCCAGGGCAAGTCCGCCGCCAAGTTCCAGAGCTTTGGTGCCGGAGAAGAAGAAGAAGAAGCTGAGGTTGGCGATTTCGGCGGCGTGTTCCGGATGTGGGAGGGTGAGCTTCTCGATTGGTTCGACGATCGCCGCGTCGCTATTCAGTCCGCTTGCTGTCCGTGCTATACATTTGGGAAGAACATGAAGCGAGCTGGTTTTGGTCCTGGCTTTTTACAGGGAACTCTTCATTTAGTTCTTGTTGCGAGCATCCTTCTCAACTGCATTGCGTTTATTATCACCAAGAAGCGCTGCTTTATATATCTGGTGGTTGCTTTCACCATTACACTAGGAACGTATTTGGGGTACTTCCGTACACAGATAAGAAACAAATTTAACATAAGGGGTAATGATAGTTCCATGGATGATTGCATCTACCACCTTGTCTGCCCATGCTGCACTTTATCTCAGATAGTATTCTCGTTCTGTGAGCAGGAAGCCAGAACATTAGAGATGAACAATGTCCAAGATGGCACCTGGCATGGCAGGGGCGATTCAATATACATAGGCAGCCTTGGAGAAGGAGGAAAGTCTTTCTTTGAGCTCCAAGCACCTCCTCTTGTGTCAATCAAGTCCCCCGACCTCTGCAACGCGCAAAAAAGTACAGCGACTAGTGCATATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_PK

Protein Analysis

269

Amino Acids

29.65

Weight (kDa)

4.83

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 81 - 198 1.3e-18 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44450 AT5G44450
fragaria_vesca FvH4_3g11170 FvH4_3g11170
malus_domestica MD05G1265300.v1.1 MD10G1243300.v1.1
prunus_persica Prupe.4G100600_v2.0.a1
pyrus_communis pycom10g20350
rosa_chinensis RchiOBHm_Chr5g0018081
rosa_laevigata RLG00000032338
rosa_multiflora Rmu_sc0010280.1_g000018
rosa_roxburghii Rroxscaffold_1G00059540
rosa_rugosa Rorug05G0040200 Rorug05G0040300 Rorug05G0040300
rosa_samantha Rh5AG133000 Rh5BG131600 Rh5CG143600 Rh5DG133300
rosa_wichuraiana Rw5G011590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 182, 656
AccII CGCG 2 cut(s) 279, 776
AccIII TCCGGA 1 cut(s) 234
AcsI RAATTY 1 cut(s) 515
AfaI GTAC 3 cut(s) 491, 499, 787
AfeI AGCGCT 1 cut(s) 434
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 1 cut(s) 681
AhlI ACTAGT 1 cut(s) 794
AjnI CCWGG 3 cut(s) 147, 349, 659
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AluBI AGCT 5 cut(s) 177, 206, 253, 338, 727
AluI AGCT 5 cut(s) 177, 206, 253, 338, 727
Alw21I GWGCWC 2 cut(s) 128, 729
Aor13HI TCCGGA 1 cut(s) 234
Aor51HI AGCGCT 1 cut(s) 434
ApeKI GCWGC 3 cut(s) 435, 579, 693
ApoI RAATTY 1 cut(s) 515
AspLEI GCGC 2 cut(s) 435, 778
AspS9I GGNCC 1 cut(s) 347
AsuHPI GGTGA 3 cut(s) 260, 415, 454
AvaII GGWCC 1 cut(s) 347
BaeI ACNNNNGTAYC 2 cut(s) 481, 514
BanI GGYRCC 2 cut(s) 182, 656
BanII GRGCYC 1 cut(s) 729
Bbv12I GWGCWC 2 cut(s) 128, 729
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 3 cut(s) 422, 566, 705
BccI CCATC 2 cut(s) 61, 647
BciT130I CCWGG 3 cut(s) 149, 351, 661
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 2 cut(s) 473, 795
BfoI RGCGCY 1 cut(s) 436
BisI GCNGC 9 cut(s) 85, 88, 91, 162, 226, 277, 436, 580, 694
BlpI GCTNAGC 1 cut(s) 38
BlsI GCNGC 9 cut(s) 86, 89, 92, 163, 227, 278, 437, 581, 695
Bme1390I CCNGG 3 cut(s) 149, 351, 661
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 2 cut(s) 184, 658
BmrFI CCNGG 3 cut(s) 149, 351, 661
BmsI GCATC 2 cut(s) 402, 564
Bpu10I CCTNAGC 1 cut(s) 207
Bpu1102I GCTNAGC 1 cut(s) 38
BsaJI CCNNGG 4 cut(s) 147, 148, 543, 697
BsaWI WCCGGW 1 cut(s) 234
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse3DI GCAATG 1 cut(s) 408
BseAI TCCGGA 1 cut(s) 234
BseBI CCWGG 3 cut(s) 149, 351, 661
BseDI CCNNGG 4 cut(s) 147, 148, 543, 697
BseGI GGATG 3 cut(s) 243, 393, 553
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 29, 198, 605
BseRI GAGGAG 3 cut(s) 74, 77, 729
BseXI GCAGC 3 cut(s) 422, 566, 705
BsgI GTGCAG 1 cut(s) 565
Bsh1236I CGCG 2 cut(s) 279, 776
Bsh1285I CGRYCG 1 cut(s) 274
BshNI GGYRCC 2 cut(s) 182, 656
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 128, 729
BsiSI CCGG 2 cut(s) 186, 235
BslFI GGGAC 1 cut(s) 742
BslI CCNNNNNNNGG 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 742
Bsp1286I GDGCHC 2 cut(s) 128, 729
Bsp13I TCCGGA 1 cut(s) 234
Bsp143I GATC 1 cut(s) 271
Bsp1720I GCTNAGC 1 cut(s) 38
Bsp19I CCATGG 1 cut(s) 543
BspCNI CTCAG 3 cut(s) 30, 199, 604
BspEI TCCGGA 1 cut(s) 234
BspFNI CGCG 2 cut(s) 279, 776
BspHI TCATGA 1 cut(s) 806
BspLI GGNNCC 2 cut(s) 184, 658
BspT107I GGYRCC 2 cut(s) 182, 656
BsrDI GCAATG 1 cut(s) 408
BssECI CCNNGG 4 cut(s) 147, 148, 543, 697
BssMI GATC 1 cut(s) 271
BssT1I CCWWGG 2 cut(s) 543, 697
Bst2UI CCWGG 3 cut(s) 149, 351, 661
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 2 cut(s) 51, 375
BstC8I GCNNGC 4 cut(s) 21, 298, 336, 391
BstDEI CTNAG 3 cut(s) 38, 207, 591
BstDSI CCRYGG 1 cut(s) 543
BstF5I GGATG 3 cut(s) 243, 393, 553
BstFNI CGCG 2 cut(s) 279, 776
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 2 cut(s) 435, 778
BstKTI GATC 1 cut(s) 274
BstMBI GATC 1 cut(s) 271
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 5 cut(s) 90, 222, 306, 579, 797
BstNI CCWGG 3 cut(s) 149, 351, 661
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 3 cut(s) 147, 349, 659
BstUI CGCG 2 cut(s) 279, 776
BstV1I GCAGC 3 cut(s) 422, 566, 705
BstXI CCANNNNNNTGG 1 cut(s) 179
BtgI CCRYGG 1 cut(s) 543
BtsCI GGATG 3 cut(s) 243, 393, 553
Cac8I GCNNGC 4 cut(s) 21, 298, 336, 391
CciI TCATGA 1 cut(s) 806
CfoI GCGC 2 cut(s) 435, 778
Cfr13I GGNCC 1 cut(s) 347
CseI GACGC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 490, 498, 786
CviAII CATG 6 cut(s) 118, 328, 544, 576, 665, 807
CviJI RGCY 9 cut(s) 76, 177, 206, 253, 338, 354, 623, 696, 727
CviKI_1 RGCY 9 cut(s) 76, 177, 206, 253, 338, 354, 623, 696, 727
CviQI GTAC 3 cut(s) 490, 498, 786
DdeI CTNAG 3 cut(s) 38, 207, 591
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
Eam1104I CTCTTC 2 cut(s) 51, 375
EarI CTCTTC 2 cut(s) 51, 375
EciI GGCGGA 3 cut(s) 68, 108, 148
Ecl136II GAGCTC 1 cut(s) 727
Eco130I CCWWGG 2 cut(s) 543, 697
Eco24I GRGCYC 1 cut(s) 729
Eco47I GGWCC 1 cut(s) 347
Eco47III AGCGCT 1 cut(s) 434
Eco53kI GAGCTC 1 cut(s) 727
EcoICRI GAGCTC 1 cut(s) 727
EcoRII CCWGG 3 cut(s) 147, 349, 659
EcoT14I CCWWGG 2 cut(s) 543, 697
EcoT38I GRGCYC 1 cut(s) 729
ErhI CCWWGG 2 cut(s) 543, 697
FaeI CATG 6 cut(s) 121, 331, 547, 579, 668, 810
FalI AAGNNNNNCTT 4 cut(s) 422, 454, 704, 736
FaqI GGGAC 1 cut(s) 742
FatI CATG 6 cut(s) 117, 327, 543, 575, 664, 806
Fnu4HI GCNGC 9 cut(s) 85, 88, 91, 162, 226, 277, 436, 580, 694
FokI GGATG 3 cut(s) 250, 380, 560
FriOI GRGCYC 1 cut(s) 729
Fsp4HI GCNGC 9 cut(s) 85, 88, 91, 162, 226, 277, 436, 580, 694
FspBI CTAG 2 cut(s) 473, 795
GlaI GCGC 2 cut(s) 434, 777
GluI GCNGC 9 cut(s) 85, 88, 91, 162, 226, 277, 436, 580, 694
HaeII RGCGCY 1 cut(s) 436
HapII CCGG 2 cut(s) 186, 235
HgaI GACGC 1 cut(s) 268
HhaI GCGC 2 cut(s) 435, 778
Hin1II CATG 6 cut(s) 121, 331, 547, 579, 668, 810
Hin6I GCGC 2 cut(s) 433, 776
HinP1I GCGC 2 cut(s) 433, 776
HinfI GANTC 1 cut(s) 677
HpaII CCGG 2 cut(s) 186, 235
HphI GGTGA 3 cut(s) 260, 415, 454
Hpy166II GTNNAC 1 cut(s) 500
Hpy188I TCNGA 1 cut(s) 594
Hpy188III TCNNGA 4 cut(s) 172, 235, 257, 807
Hpy8I GTNNAC 1 cut(s) 500
Hpy99I CGWCG 3 cut(s) 137, 272, 284
HpyAV CCTTC 2 cut(s) 407, 698
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 479
HpyCH4V TGCA 5 cut(s) 408, 555, 582, 771, 800
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 222, 306, 579, 797
HpyF3I CTNAG 3 cut(s) 38, 207, 591
HpySE526I ACGT 1 cut(s) 479
Hsp92II CATG 6 cut(s) 121, 331, 547, 579, 668, 810
HspAI GCGC 2 cut(s) 433, 776
Kpn2I TCCGGA 1 cut(s) 234
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 2 cut(s) 131, 732
Lsp1109I GCAGC 3 cut(s) 422, 566, 705
LweI GCATC 2 cut(s) 402, 564
MaeI CTAG 2 cut(s) 473, 795
MaeII ACGT 1 cut(s) 479
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 7 cut(s) 68, 203, 206, 209, 212, 334, 362
MhlI GDGCHC 2 cut(s) 128, 729
MluCI AATT 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 87
MroI TCCGGA 1 cut(s) 234
MseI TTAA 1 cut(s) 519
MslI CAYNNNNRTG 1 cut(s) 805
MspI CCGG 2 cut(s) 186, 235
MspR9I CCNGG 3 cut(s) 149, 351, 661
MvaI CCWGG 3 cut(s) 149, 351, 661
MvnI CGCG 2 cut(s) 279, 776
MwoI GCNNNNNNNGC 5 cut(s) 90, 222, 306, 579, 797
NcoI CCATGG 1 cut(s) 543
NdeII GATC 1 cut(s) 271
NlaIII CATG 6 cut(s) 121, 331, 547, 579, 668, 810
NlaIV GGNNCC 2 cut(s) 184, 658
NspI RCATGY 1 cut(s) 121
PagI TCATGA 1 cut(s) 806
PasI CCCWGGG 1 cut(s) 148
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 276
PfeI GAWTC 1 cut(s) 677
PkrI GCNGC 9 cut(s) 86, 89, 92, 163, 227, 278, 437, 581, 695
Ple19I CGATCG 1 cut(s) 274
Psp124BI GAGCTC 1 cut(s) 729
Psp6I CCWGG 3 cut(s) 147, 349, 659
PspGI CCWGG 3 cut(s) 147, 349, 659
PspN4I GGNNCC 2 cut(s) 184, 658
PspPI GGNCC 1 cut(s) 347
PsrI GAACNNNNNNTAC 2 cut(s) 523, 555
PvuI CGATCG 1 cut(s) 274
RsaI GTAC 3 cut(s) 491, 499, 787
RsaNI GTAC 3 cut(s) 490, 498, 786
RseI CAYNNNNRTG 1 cut(s) 805
SacI GAGCTC 1 cut(s) 729
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 9 cut(s) 85, 88, 91, 162, 226, 277, 436, 580, 694
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 1 cut(s) 347
ScrFI CCNGG 3 cut(s) 149, 351, 661
SduI GDGCHC 2 cut(s) 128, 729
SfaNI GCATC 2 cut(s) 402, 564
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 805
SpeI ACTAGT 1 cut(s) 794
Sse9I AATT 1 cut(s) 515
SspMI CTAG 2 cut(s) 473, 795
SstI GAGCTC 1 cut(s) 729
StyD4I CCNGG 3 cut(s) 147, 349, 659
StyI CCWWGG 2 cut(s) 543, 697
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 482
TaqI TCGA 3 cut(s) 114, 258, 267
TasI AATT 1 cut(s) 515
TatI WGTACW 1 cut(s) 785
TauI GCSGC 6 cut(s) 87, 90, 93, 164, 228, 279
TfiI GAWTC 1 cut(s) 677
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TseI GCWGC 3 cut(s) 435, 579, 693
TspDTI ATGAA 4 cut(s) 344, 362, 653, 795
TspGWI ACGGA 2 cut(s) 294, 485
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 1 cut(s) 515
XceI RCATGY 1 cut(s) 121
XspI CTAG 2 cut(s) 473, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.