AT5G46150

LEM3 (ligand-effect modulator 3) family / CDC50 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005969
Physical Location & Seq
Reverse (-)
18707546 .. 18710722
3177 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46150.4

Sequence Viewer

Length: 1032 bp
ATGATGGAAGTGGAAGGATCGATGAATCGTGCTCCTGATCAATCATCCTTCTTACGATCAAGACGATCCAAAGCTCTGTATCAGTTTAAGCAGCAGAAACTCCCAGCTTGTAAGCCAGTCTTAACACCAATATCGGTTATAACAGTGTTTATGTTAATGGGTTTTGTTTTCATTCCCATTGGTCTCATTACTCTTCGTGCTTCTCGTGATGCTATTGAAATTATAGACCGGTATGATGTTGAGTGCATTCCTGAAGAATATAGAACCAACAAACTGTTGTATATAACTGATTCATCTATCCCAAAGAATTGTACGCGCTACTTGAAGGTACAAAAGTATATGAAAGCTCCCATTTTTATCTACTACCAGCTTGATAACTACTATCAAAACCATCGCCGGTATGTAAAGAGTAGAAGTGATCAGCAACTATTACATGGACTGGAGTATAGCCACACAAGTTCTTGTGAACCCGAGGAGTCATCTAATGGTCTCCCAATTGTTCCTTGTGGGTTAATTGCTTGGAGTATGTTCAATGATACATTTACGTTTTCTCGCGAAAGAACAAAACTGAATGTGAGCCGGAATAACATTGCGTGGAAGAGTGACCGCGAGCACAAGTTTGGAAAGAATGTGTATCCTATCAATTTCCAGAACGGAACTTTGATTGGTGGAGCAAAGTTGGATCCAAAAATTCCACTAAGTGACCAAGAGGACTTTATAGTGTGGATGCGAGCAGCTGCTTTACTAAGCTTCCGGAAATTATATGGAAGAATCGAAGAGGACTTGGAACCAGGAAAAGTTGTTGAAGTAAATTTGATGAACAATTACAACACTTATAGCTTTAGTGGGCAGAAGAAGCTTATTCTCTCCACATCAAATTGGTTAGGAGGAAGAAATGATTTCCTGGGTATCACCTATCTTGTCGTTGGTTCCTCTTCCATAGTCATATCCATCATCTTCATGTTGCTCCATTTGAAAAATCCAAGGCCATACGGTGACAATTCTTGGAACAAGAAAAGCCTTTCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

343

Amino Acids

39.55

Weight (kDa)

9.35

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 66 - 216 6.1e-44 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 140
AccII CGCG 3 cut(s) 316, 555, 609
AccIII TCCGGA 1 cut(s) 753
AciI CCGC 1 cut(s) 607
AclWI GGATC 4 cut(s) 25, 60, 677, 690
AcsI RAATTY 2 cut(s) 690, 811
AcuI CTGAAG 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 701
AfaI GTAC 2 cut(s) 313, 330
AgeI ACCGGT 1 cut(s) 228
AgsI TTSAA 6 cut(s) 218, 325, 532, 806, 976, 1026
AjnI CCWGG 2 cut(s) 790, 903
AluBI AGCT 8 cut(s) 74, 107, 347, 370, 737, 750, 840, 859
AluI AGCT 8 cut(s) 74, 107, 347, 370, 737, 750, 840, 859
Alw21I GWGCWC 2 cut(s) 34, 615
Alw26I GTCTC 2 cut(s) 188, 494
AlwI GGATC 4 cut(s) 25, 60, 677, 690
Ama87I CYCGRG 1 cut(s) 470
Aor13HI TCCGGA 1 cut(s) 753
AoxI GGCC 1 cut(s) 986
ApeKI GCWGC 3 cut(s) 91, 734, 737
ApoI RAATTY 2 cut(s) 690, 811
AsiGI ACCGGT 1 cut(s) 228
AspLEI GCGC 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 904, 1007
AvaI CYCGRG 1 cut(s) 470
BaeI ACNNNNGTAYC 2 cut(s) 320, 353
BamHI GGATCC 1 cut(s) 682
BauI CACGAG 1 cut(s) 204
Bbv12I GWGCWC 2 cut(s) 34, 615
BbvI GCAGC 3 cut(s) 103, 724, 746
BccI CCATC 2 cut(s) 399, 959
BciT130I CCWGG 2 cut(s) 792, 905
BciVI GTATCC 1 cut(s) 645
BclI TGATCA 2 cut(s) 37, 418
BcoDI GTCTC 2 cut(s) 188, 494
BfuI GTATCC 1 cut(s) 645
BisI GCNGC 3 cut(s) 92, 735, 738
BlsI GCNGC 3 cut(s) 93, 736, 739
Bme1390I CCNGG 2 cut(s) 792, 905
BmeT110I CYCGRG 1 cut(s) 470
BmiI GGNNCC 3 cut(s) 684, 789, 931
BmrFI CCNGG 2 cut(s) 792, 905
BmsI GCATC 2 cut(s) 199, 717
BpmI CTGGAG 1 cut(s) 461
Bsa29I ATCGAT 1 cut(s) 20
BsaI GGTCTC 2 cut(s) 188, 494
BsaJI CCNNGG 3 cut(s) 471, 904, 983
BsaWI WCCGGW 2 cut(s) 228, 753
Bse118I RCCGGY 2 cut(s) 228, 396
Bse1I ACTGG 2 cut(s) 116, 444
Bse3DI GCAATG 1 cut(s) 588
BseAI TCCGGA 1 cut(s) 753
BseBI CCWGG 2 cut(s) 792, 905
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 3 cut(s) 471, 904, 983
BseGI GGATG 2 cut(s) 44, 732
BseMI GCAATG 1 cut(s) 588
BseNI ACTGG 2 cut(s) 116, 444
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 3 cut(s) 103, 724, 746
BseYI CCCAGC 1 cut(s) 103
Bsh1236I CGCG 3 cut(s) 316, 555, 609
BshFI GGCC 1 cut(s) 988
BshTI ACCGGT 1 cut(s) 228
BshVI ATCGAT 1 cut(s) 20
BsiHKAI GWGCWC 2 cut(s) 34, 615
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 4 cut(s) 229, 397, 580, 754
BsmAI GTCTC 2 cut(s) 188, 494
BsmI GAATGC 1 cut(s) 246
BsnI GGCC 1 cut(s) 988
Bso31I GGTCTC 2 cut(s) 188, 494
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 34, 615
Bsp13I TCCGGA 1 cut(s) 753
Bsp143I GATC 6 cut(s) 17, 37, 56, 65, 418, 682
Bsp68I TCGCGA 1 cut(s) 555
BspACI CCGC 1 cut(s) 607
BspANI GGCC 1 cut(s) 988
BspDI ATCGAT 1 cut(s) 20
BspEI TCCGGA 1 cut(s) 753
BspFNI CGCG 3 cut(s) 316, 555, 609
BspLI GGNNCC 3 cut(s) 684, 789, 931
BspPI GGATC 4 cut(s) 25, 60, 677, 690
BspTNI GGTCTC 2 cut(s) 188, 494
BsrDI GCAATG 1 cut(s) 588
BsrFI RCCGGY 2 cut(s) 228, 396
BsrI ACTGG 2 cut(s) 116, 444
BssAI RCCGGY 2 cut(s) 228, 396
BssECI CCNNGG 3 cut(s) 471, 904, 983
BssMI GATC 6 cut(s) 17, 37, 56, 65, 418, 682
BssSI CACGAG 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 983
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 2 cut(s) 792, 905
Bst4CI ACNGT 3 cut(s) 145, 276, 995
Bst6I CTCTTC 4 cut(s) 198, 593, 771, 940
BstC8I GCNNGC 2 cut(s) 611, 732
BstDEI CTNAG 2 cut(s) 698, 746
BstF5I GGATG 2 cut(s) 44, 732
BstFNI CGCG 3 cut(s) 316, 555, 609
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 6 cut(s) 20, 40, 59, 68, 421, 685
BstMAI GTCTC 2 cut(s) 188, 494
BstMBI GATC 6 cut(s) 17, 37, 56, 65, 418, 682
BstMWI GCNNNNNNNGC 1 cut(s) 856
BstNI CCWGG 2 cut(s) 792, 905
BstSCI CCNGG 2 cut(s) 790, 903
BstUI CGCG 3 cut(s) 316, 555, 609
BstV1I GCAGC 3 cut(s) 103, 724, 746
BstX2I RGATCY 1 cut(s) 682
BstYI RGATCY 1 cut(s) 682
Bsu15I ATCGAT 1 cut(s) 20
BsuI GTATCC 1 cut(s) 645
BsuRI GGCC 1 cut(s) 988
BsuTUI ATCGAT 1 cut(s) 20
BtgZI GCGATG 1 cut(s) 377
BtsCI GGATG 2 cut(s) 44, 732
BtsIMutI CAGTG 1 cut(s) 150
BtuMI TCGCGA 1 cut(s) 555
Cac8I GCNNGC 2 cut(s) 611, 732
CfoI GCGC 1 cut(s) 318
Cfr10I RCCGGY 2 cut(s) 228, 396
ClaI ATCGAT 1 cut(s) 20
Csp6I GTAC 2 cut(s) 312, 329
CspAI ACCGGT 1 cut(s) 228
CviAII CATG 2 cut(s) 434, 961
CviQI GTAC 2 cut(s) 312, 329
DdeI CTNAG 2 cut(s) 698, 746
DpnI GATC 6 cut(s) 19, 39, 58, 67, 420, 684
DpnII GATC 6 cut(s) 17, 37, 56, 65, 418, 682
DraIII CACNNNGTG 1 cut(s) 701
Eam1104I CTCTTC 4 cut(s) 198, 593, 771, 940
EarI CTCTTC 4 cut(s) 198, 593, 771, 940
Eco130I CCWWGG 1 cut(s) 983
Eco31I GGTCTC 2 cut(s) 188, 494
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 470
EcoRII CCWGG 2 cut(s) 790, 903
EcoT14I CCWWGG 1 cut(s) 983
ErhI CCWWGG 1 cut(s) 983
FaeI CATG 2 cut(s) 437, 964
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FatI CATG 2 cut(s) 433, 960
FbaI TGATCA 2 cut(s) 37, 418
Fnu4HI GCNGC 3 cut(s) 92, 735, 738
FokI GGATG 2 cut(s) 31, 739
Fsp4HI GCNGC 3 cut(s) 92, 735, 738
GlaI GCGC 1 cut(s) 317
GluI GCNGC 3 cut(s) 92, 735, 738
GsaI CCCAGC 1 cut(s) 107
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 1 cut(s) 988
HapII CCGG 4 cut(s) 229, 397, 580, 754
HhaI GCGC 1 cut(s) 318
Hin1II CATG 2 cut(s) 437, 964
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HindIII AAGCTT 2 cut(s) 748, 857
HinfI GANTC 4 cut(s) 25, 290, 476, 771
HpaII CCGG 4 cut(s) 229, 397, 580, 754
HphI GGTGA 2 cut(s) 904, 1007
Hpy166II GTNNAC 1 cut(s) 467
Hpy188III TCNNGA 7 cut(s) 35, 60, 206, 251, 554, 649, 754
Hpy8I GTNNAC 1 cut(s) 467
HpyAV CCTTC 3 cut(s) 8, 58, 319
HpyCH4III ACNGT 3 cut(s) 145, 276, 995
HpyCH4IV ACGT 1 cut(s) 545
HpyCH4V TGCA 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 856
HpyF3I CTNAG 2 cut(s) 698, 746
HpySE526I ACGT 1 cut(s) 545
Hsp92II CATG 2 cut(s) 437, 964
HspAI GCGC 1 cut(s) 316
Kpn2I TCCGGA 1 cut(s) 753
Ksp22I TGATCA 2 cut(s) 37, 418
Kzo9I GATC 6 cut(s) 17, 37, 56, 65, 418, 682
LmnI GCTCC 4 cut(s) 37, 352, 671, 972
Lsp1109I GCAGC 3 cut(s) 103, 724, 746
LweI GCATC 2 cut(s) 199, 717
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 3 cut(s) 602, 701, 995
MalI GATC 6 cut(s) 19, 39, 58, 67, 420, 684
MboI GATC 6 cut(s) 17, 37, 56, 65, 418, 682
MboII GAAGA 9 cut(s) 185, 266, 610, 780, 788, 865, 903, 927, 949
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 682
MhlI GDGCHC 2 cut(s) 34, 615
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 1 cut(s) 660
MnlI CCTC 5 cut(s) 466, 703, 772, 881, 943
MroI TCCGGA 1 cut(s) 753
MseI TTAA 4 cut(s) 87, 122, 155, 512
MslI CAYNNNNRTG 1 cut(s) 959
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 4 cut(s) 229, 397, 580, 754
MspR9I CCNGG 2 cut(s) 792, 905
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 246
MvaI CCWGG 2 cut(s) 792, 905
MvnI CGCG 3 cut(s) 316, 555, 609
MwoI GCNNNNNNNGC 1 cut(s) 856
NdeII GATC 6 cut(s) 17, 37, 56, 65, 418, 682
NlaIII CATG 2 cut(s) 437, 964
NlaIV GGNNCC 3 cut(s) 684, 789, 931
NmuCI GTSAC 3 cut(s) 602, 701, 995
NruI TCGCGA 1 cut(s) 555
PcsI WCGNNNNNNNCGW 2 cut(s) 61, 202
PctI GAATGC 1 cut(s) 246
PfeI GAWTC 3 cut(s) 25, 290, 771
PinAI ACCGGT 1 cut(s) 228
PkrI GCNGC 3 cut(s) 93, 736, 739
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PsiI TTATAA 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 790, 903
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 2 cut(s) 790, 903
PspN4I GGNNCC 3 cut(s) 684, 789, 931
PsuI RGATCY 1 cut(s) 682
PvuII CAGCTG 1 cut(s) 737
RruI TCGCGA 1 cut(s) 555
RsaI GTAC 2 cut(s) 313, 330
RsaNI GTAC 2 cut(s) 312, 329
RseI CAYNNNNRTG 1 cut(s) 959
SaqAI TTAA 4 cut(s) 87, 122, 155, 512
SatI GCNGC 3 cut(s) 92, 735, 738
Sau3AI GATC 6 cut(s) 17, 37, 56, 65, 418, 682
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 792, 905
SduI GDGCHC 2 cut(s) 34, 615
SfaNI GCATC 2 cut(s) 199, 717
SmiMI CAYNNNNRTG 1 cut(s) 959
SsiI CCGC 1 cut(s) 607
StyD4I CCNGG 2 cut(s) 790, 903
StyI CCWWGG 1 cut(s) 983
TaaI ACNGT 3 cut(s) 145, 276, 995
TaiI ACGT 1 cut(s) 548
TaqI TCGA 2 cut(s) 20, 774
TfiI GAWTC 3 cut(s) 25, 290, 771
Tru1I TTAA 4 cut(s) 87, 122, 155, 512
Tru9I TTAA 4 cut(s) 87, 122, 155, 512
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 3 cut(s) 602, 701, 995
TseI GCWGC 3 cut(s) 91, 734, 737
Tsp45I GTSAC 3 cut(s) 602, 701, 995
TspDTI ATGAA 6 cut(s) 38, 160, 282, 356, 833, 949
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 1 cut(s) 150
XapI RAATTY 2 cut(s) 690, 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.