RchiOBHm_Chr5g0014591

ALA-interacting subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005969
Physical Location & Seq
Forward (+)
10058508 .. 10062566
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29504

Sequence Viewer

Length: 1002 bp
ATGGAAGTGGATGGAGGAAGAATCTTAAAAGTATCTAGTGGTGCTCAATCTATACCACGACATTCCAGAAAACATAAAGCTTTCTATCAATTTACTCAGCAGAGTCTCCCTGCTTGTAAACCTGTCCTCACACCAGCATGGGTGATTACTACATTTTTATTGATGGGTGTCATTTTCATACCTATTGGGCTTATTACTCTTCATGCCTCTGAGAATGTTGTTGAAATAGTGGACCGATATGATACTGAATGTGTCCCAGAAGCATTCAGAAGCAATAAGGTGGCATATATCAAAGATAACTCGATTCCCAAAAACTGTTCACGGTACTTGAAGGTCTACAAGCATATGAAAGCTCCAATAAATATCTACTATCAGCTTGATAACTACTTTCAGAACCACAGACGGTATGTCAAAAGTAAAAATGATCGACAGCTTTTACATGGATTGGGGTACAATGACACAGGCTCCTGTAAACCTGAGGAGTCCAACAATGGTCTTCCAATTGTTCCTTGTGGTTTAATAGCATGGAGTTTGTTTAATGATACATATACTCTTATTCGTGGAAGATCAGAGTTAAAGGTCAACAGGAAGAACATAGCATGGAAGAGTGACCGTGATCACAAGTTTGGGAAGAGTGTATATCCCTTCAATTTTCAGAACGGCACCTTGATGGGTGGTGGAAAGCTACACCCAAGAATCCCTCTAAGTGATCAAGAAGATCTTATTGTATGGATGCGGACAGCTGCGCTCCCCAGTTTCCGTAAGTTGTATGGTAGAATTGAAGAGGATTTGAATGCAGATGATGTTGTGGTAGTCAATGTAACGAACAACTACAATACTTATAGTTTTGGAGGGAAAAAGAAGCTTATTCTTTCAACAACAGGCTGGTTAGGAGGAAAGAACAACTTCCTTGGGCTTGCCTGCACTTCTGTTGGTTCAAGTTCCATATTCATCTCTATTGTCTTCTTGCTGCTCCACATGAAAAATCCCAGGATTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

333

Amino Acids

37.74

Weight (kDa)

9.59

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 67 - 331 1.1e-80 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 662
AccI GTMKAC 1 cut(s) 336
AciI CCGC 1 cut(s) 736
AfaI GTAC 2 cut(s) 326, 452
AgsI TTSAA 7 cut(s) 224, 331, 649, 782, 793, 876, 939
AhdI GACNNNNNGTC 1 cut(s) 407
AjnI CCWGG 1 cut(s) 989
AluBI AGCT 7 cut(s) 80, 353, 376, 433, 685, 743, 865
AluI AGCT 7 cut(s) 80, 353, 376, 433, 685, 743, 865
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 110
ApeKI GCWGC 2 cut(s) 743, 970
Asp700I GAANNNNTTC 1 cut(s) 905
AspLEI GCGC 1 cut(s) 748
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 232
AxyI CCTNAGG 1 cut(s) 477
BanI GGYRCC 1 cut(s) 662
BbsI GAAGAC 2 cut(s) 488, 955
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 2 cut(s) 730, 957
BccI CCATC 3 cut(s) 5, 157, 664
BceAI ACGGC 1 cut(s) 676
BciT130I CCWGG 1 cut(s) 991
BclI TGATCA 2 cut(s) 616, 709
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 1 cut(s) 36
BglII AGATCT 1 cut(s) 718
BisI GCNGC 2 cut(s) 744, 971
BlsI GCNGC 2 cut(s) 745, 972
Bme1390I CCNGG 1 cut(s) 991
Bme18I GGWCC 1 cut(s) 232
BmeRI GACNNNNNGTC 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 2 cut(s) 466, 664
BmrFI CCNGG 1 cut(s) 991
BmrI ACTGGG 1 cut(s) 747
BmsI GCATC 1 cut(s) 723
BmuI ACTGGG 1 cut(s) 747
BpiI GAAGAC 2 cut(s) 488, 955
BsaJI CCNNGG 2 cut(s) 910, 989
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bse1I ACTGG 1 cut(s) 753
Bse21I CCTNAGG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 991
BseDI CCNNGG 2 cut(s) 910, 989
BseGI GGATG 2 cut(s) 16, 738
BseMII CTCAG 3 cut(s) 110, 201, 468
BseNI ACTGG 1 cut(s) 753
BseRI GAGGAG 1 cut(s) 494
BseXI GCAGC 2 cut(s) 730, 957
BsgI GTGCAG 1 cut(s) 907
BshNI GGYRCC 1 cut(s) 662
BsiHKAI GWGCWC 1 cut(s) 46
BslFI GGGAC 1 cut(s) 239
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 2 cut(s) 263, 799
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 5 cut(s) 424, 566, 616, 709, 718
BspACI CCGC 1 cut(s) 736
BspCNI CTCAG 3 cut(s) 109, 202, 469
BspLI GGNNCC 2 cut(s) 466, 664
BspT107I GGYRCC 1 cut(s) 662
BsrI ACTGG 1 cut(s) 753
BssECI CCNNGG 2 cut(s) 910, 989
BssMI GATC 5 cut(s) 424, 566, 616, 709, 718
BssT1I CCWWGG 1 cut(s) 910
Bst2UI CCWGG 1 cut(s) 991
Bst4CI ACNGT 4 cut(s) 317, 324, 405, 614
Bst6I CTCTTC 4 cut(s) 204, 599, 626, 777
BstC8I GCNNGC 2 cut(s) 918, 922
BstDEI CTNAG 4 cut(s) 96, 210, 477, 704
BstF5I GGATG 2 cut(s) 16, 738
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 5 cut(s) 427, 569, 619, 712, 721
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 5 cut(s) 424, 566, 616, 709, 718
BstNI CCWGG 1 cut(s) 991
BstSCI CCNGG 1 cut(s) 989
BstV1I GCAGC 2 cut(s) 730, 957
BstV2I GAAGAC 2 cut(s) 488, 955
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
Bsu36I CCTNAGG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 16, 738
Cac8I GCNNGC 2 cut(s) 918, 922
CfoI GCGC 1 cut(s) 748
Cfr13I GGNCC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 325, 451
CviAII CATG 6 cut(s) 138, 203, 440, 525, 600, 979
CviQI GTAC 2 cut(s) 325, 451
DdeI CTNAG 4 cut(s) 96, 210, 477, 704
DpnI GATC 5 cut(s) 426, 568, 618, 711, 720
DpnII GATC 5 cut(s) 424, 566, 616, 709, 718
DriI GACNNNNNGTC 1 cut(s) 407
Eam1104I CTCTTC 4 cut(s) 204, 599, 626, 777
Eam1105I GACNNNNNGTC 1 cut(s) 407
EarI CTCTTC 4 cut(s) 204, 599, 626, 777
Eco130I CCWWGG 1 cut(s) 910
Eco47I GGWCC 1 cut(s) 232
Eco81I CCTNAGG 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 989
EcoT14I CCWWGG 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 910
FaeI CATG 6 cut(s) 141, 206, 443, 528, 603, 982
FalI AAGNNNNNCTT 4 cut(s) 705, 737, 890, 922
FaqI GGGAC 1 cut(s) 239
FatI CATG 6 cut(s) 137, 202, 439, 524, 599, 978
FauNDI CATATG 1 cut(s) 345
FbaI TGATCA 2 cut(s) 616, 709
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 744, 971
FokI GGATG 2 cut(s) 23, 745
Fsp4HI GCNGC 2 cut(s) 744, 971
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 747
GluI GCNGC 2 cut(s) 744, 971
HhaI GCGC 1 cut(s) 748
Hin1II CATG 6 cut(s) 141, 206, 443, 528, 603, 982
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HindIII AAGCTT 2 cut(s) 78, 863
HinfI GANTC 6 cut(s) 21, 103, 304, 482, 696, 994
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 6 cut(s) 119, 232, 320, 337, 473, 583
Hpy188I TCNGA 5 cut(s) 211, 269, 393, 571, 657
Hpy188III TCNNGA 2 cut(s) 66, 713
Hpy8I GTNNAC 6 cut(s) 119, 232, 320, 337, 473, 583
HpyAV CCTTC 2 cut(s) 325, 655
HpyCH4III ACNGT 4 cut(s) 317, 324, 405, 614
HpyCH4V TGCA 2 cut(s) 797, 924
HpyF3I CTNAG 4 cut(s) 96, 210, 477, 704
Hsp92II CATG 6 cut(s) 141, 206, 443, 528, 603, 982
HspAI GCGC 1 cut(s) 746
Ksp22I TGATCA 2 cut(s) 616, 709
Kzo9I GATC 5 cut(s) 424, 566, 616, 709, 718
LmnI GCTCC 4 cut(s) 358, 470, 753, 978
Lsp1109I GCAGC 2 cut(s) 730, 957
LweI GCATC 1 cut(s) 723
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 608, 820
MalI GATC 5 cut(s) 426, 568, 618, 711, 720
MboI GATC 5 cut(s) 424, 566, 616, 709, 718
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 4 cut(s) 89, 501, 649, 777
MlyI GAGTC 2 cut(s) 112, 491
MmeI TCCRAC 1 cut(s) 510
MnlI CCTC 8 cut(s) 8, 137, 217, 472, 711, 778, 845, 887
MroXI GAANNNNTTC 1 cut(s) 905
MseI TTAA 4 cut(s) 26, 518, 537, 575
MslI CAYNNNNRTG 2 cut(s) 136, 668
MspA1I CMGCKG 1 cut(s) 743
MspR9I CCNGG 1 cut(s) 991
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 2 cut(s) 263, 799
MvaI CCWGG 1 cut(s) 991
NdeI CATATG 1 cut(s) 345
NdeII GATC 5 cut(s) 424, 566, 616, 709, 718
NlaIII CATG 6 cut(s) 141, 206, 443, 528, 603, 982
NlaIV GGNNCC 2 cut(s) 466, 664
NmuCI GTSAC 1 cut(s) 608
PctI GAATGC 2 cut(s) 263, 799
PdmI GAANNNNTTC 1 cut(s) 905
PfeI GAWTC 4 cut(s) 21, 304, 696, 994
PkrI GCNGC 2 cut(s) 745, 972
PleI GAGTC 2 cut(s) 111, 490
PpsI GAGTC 2 cut(s) 111, 490
Psp6I CCWGG 1 cut(s) 989
PspGI CCWGG 1 cut(s) 989
PspN4I GGNNCC 2 cut(s) 466, 664
PspPI GGNCC 1 cut(s) 232
PsuI RGATCY 1 cut(s) 718
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 2 cut(s) 326, 452
RsaNI GTAC 2 cut(s) 325, 451
RseI CAYNNNNRTG 2 cut(s) 136, 668
SaqAI TTAA 4 cut(s) 26, 518, 537, 575
SatI GCNGC 2 cut(s) 744, 971
Sau3AI GATC 5 cut(s) 424, 566, 616, 709, 718
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 2 cut(s) 112, 491
ScrFI CCNGG 1 cut(s) 991
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 1 cut(s) 723
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 136, 668
Sse9I AATT 4 cut(s) 89, 501, 649, 777
SsiI CCGC 1 cut(s) 736
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 989
StyI CCWWGG 1 cut(s) 910
TaaI ACNGT 4 cut(s) 317, 324, 405, 614
TaqI TCGA 2 cut(s) 302, 427
TaqII GACCGA 1 cut(s) 249
TasI AATT 4 cut(s) 89, 501, 649, 777
TfiI GAWTC 4 cut(s) 21, 304, 696, 994
Tru1I TTAA 4 cut(s) 26, 518, 537, 575
Tru9I TTAA 4 cut(s) 26, 518, 537, 575
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 2 cut(s) 743, 970
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 5 cut(s) 166, 191, 362, 940, 995
TspGWI ACGGA 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 232
XmiI GTMKAC 1 cut(s) 336
XmnI GAANNNNTTC 1 cut(s) 905
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.