Prupe.4G084400_v2.0.a1

ALA-interacting subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0005969
Physical Location & Seq
Forward (+)
4122952 .. 4128334
5383 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G084400.1

Sequence Viewer

Length: 999 bp
ATGGAAGTGGATGGAAGTGCCTCAACAGTTTCTAGGGGTGCTCAATCAATTCCACTACAATCCAGGCGGCAGAGAGCTTTCTATCAGTTTACACAGCAGAGCCTCCCCGCTTGTAAGCCTGTCCTCACACCAGCATGGGTGATTACTACATTTTTATTGGTGGGTGCCATTTTCATACCAGTTGGGCTTATTACACTTCATGCCTCCCAGAACGTTGTAGAAATTGTGGACCAATATGATACAGAGTGTGTCCCGGAGGCATTCAAGGGCAATAAGGTGGCCTACATCAAAGATAACTTCATCTCTAAAAACTGTTCTCGGTACTTGAAGGTTTACAAGCATATGAAAGCTCCAATTTATATCTACTATCAGCTTGATAACTACTATCAAAACCACCGACGGTATGTCAAAAGTAGAAGTGATCAGCAGCTTTTACATGGATTGGGGTACAATGACACAAGCTCCTGTAAACCTGAGGAGTCCAAGAATGGTCTTCCAACTGTTCCTTGTGGCTTGATAGCTTGGAGTTTGTTTAATGATACTTATACTTTTATTCGTGGAAGAACTGAATTAAAAGTCAACAGGAAGAACATAGCATGGAAGAGTGACCGTGATCACAAATTTGGGAAGCATGTATATCCCTTTAATTTCCAGAACGGGACCTTGATCGGTGGTGGAAAGCTAGATCCAAGAATTCCTCTAAGCGATCAAGAAGATCTTATTGTATGGATGCGGACAGCTGCGCTCCCCAGTTTCCGGAAGCTGTATGGTAGAATTGAAGAGGATTTGGATGCAGATGATGTTCTCTTAGTCAATCTAACGAACAACTACAATACTTACAGTTTTGGTGGGAAAAAGAAGATTGTCCTTTCAACATCAAGCTGGTTAGGAGGAAAGAATGACTTCCTTGGGCTTGCCTATACTTTTGTTGGTTCGACTTCCATATTCATCTCTGTTGTCTTCTTGTTGCTACATATGAAAAATCCAAGAACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

333

Amino Acids

37.75

Weight (kDa)

9.35

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AccIII TCCGGA 1 cut(s) 756
AciI CCGC 3 cut(s) 67, 108, 733
AclI AACGTT 1 cut(s) 213
AclWI GGATC 1 cut(s) 680
AcsI RAATTY 2 cut(s) 620, 693
AfaI GTAC 2 cut(s) 323, 449
AfiI CCNNNNNNNGG 1 cut(s) 756
AgsI TTSAA 4 cut(s) 265, 328, 779, 873
AhdI GACNNNNNGTC 1 cut(s) 404
AjnI CCWGG 1 cut(s) 62
Alw21I GWGCWC 1 cut(s) 43
AlwI GGATC 1 cut(s) 680
Aor13HI TCCGGA 1 cut(s) 756
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 2 cut(s) 427, 740
ApoI RAATTY 2 cut(s) 620, 693
Asp700I GAANNNNTTC 1 cut(s) 902
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 2 cut(s) 229, 660
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 1 cut(s) 151
AvaII GGWCC 2 cut(s) 229, 660
AxyI CCTNAGG 1 cut(s) 474
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 2 cut(s) 485, 952
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 2 cut(s) 439, 727
BccI CCATC 1 cut(s) 5
BciT130I CCWGG 1 cut(s) 64
BclI TGATCA 2 cut(s) 421, 613
BcnI CCSGG 1 cut(s) 254
BfaI CTAG 2 cut(s) 33, 683
BglII AGATCT 1 cut(s) 715
BisI GCNGC 3 cut(s) 68, 428, 741
BlsI GCNGC 3 cut(s) 69, 429, 742
Bme1390I CCNGG 2 cut(s) 64, 254
Bme18I GGWCC 2 cut(s) 229, 660
BmeRI GACNNNNNGTC 1 cut(s) 404
BmgT120I GGNCC 2 cut(s) 229, 660
BmiI GGNNCC 2 cut(s) 166, 661
BmrFI CCNGG 2 cut(s) 64, 254
BmrI ACTGGG 1 cut(s) 744
BmsI GCATC 2 cut(s) 720, 781
BmuI ACTGGG 1 cut(s) 744
BpiI GAAGAC 2 cut(s) 485, 952
BpuMI CCSGG 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 907
BsaWI WCCGGW 1 cut(s) 756
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 756
Bse1I ACTGG 2 cut(s) 179, 750
Bse21I CCTNAGG 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 756
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 907
BseGI GGATG 3 cut(s) 16, 735, 796
BseLI CCNNNNNNNGG 1 cut(s) 756
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 2 cut(s) 179, 750
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 2 cut(s) 439, 727
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 2 cut(s) 254, 757
BslFI GGGAC 2 cut(s) 236, 673
BslI CCNNNNNNNGG 1 cut(s) 756
BsmFI GGGAC 2 cut(s) 236, 673
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 43
Bsp13I TCCGGA 1 cut(s) 756
Bsp143I GATC 6 cut(s) 421, 613, 666, 685, 706, 715
BspACI CCGC 3 cut(s) 67, 108, 733
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 1 cut(s) 466
BspEI TCCGGA 1 cut(s) 756
BspLI GGNNCC 2 cut(s) 166, 661
BspPI GGATC 1 cut(s) 680
BspT107I GGYRCC 1 cut(s) 164
BsrI ACTGG 2 cut(s) 179, 750
BssECI CCNNGG 1 cut(s) 907
BssMI GATC 6 cut(s) 421, 613, 666, 685, 706, 715
BssT1I CCWWGG 1 cut(s) 907
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 6 cut(s) 28, 314, 402, 502, 611, 842
Bst6I CTCTTC 2 cut(s) 596, 774
BstC8I GCNNGC 1 cut(s) 915
BstDEI CTNAG 3 cut(s) 474, 701, 808
BstF5I GGATG 3 cut(s) 16, 735, 796
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 6 cut(s) 424, 616, 669, 688, 709, 718
BstMBI GATC 6 cut(s) 421, 613, 666, 685, 706, 715
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 635
BstSCI CCNGG 2 cut(s) 62, 252
BstV1I GCAGC 2 cut(s) 439, 727
BstV2I GAAGAC 2 cut(s) 485, 952
BstX2I RGATCY 2 cut(s) 685, 715
BstYI RGATCY 2 cut(s) 685, 715
Bsu36I CCTNAGG 1 cut(s) 474
BsuRI GGCC 1 cut(s) 281
BtsCI GGATG 3 cut(s) 16, 735, 796
Cac8I GCNNGC 1 cut(s) 915
CfoI GCGC 1 cut(s) 745
Cfr13I GGNCC 2 cut(s) 229, 660
Csp6I GTAC 2 cut(s) 322, 448
CviAII CATG 5 cut(s) 135, 200, 437, 597, 632
CviQI GTAC 2 cut(s) 322, 448
DdeI CTNAG 3 cut(s) 474, 701, 808
DpnI GATC 6 cut(s) 423, 615, 668, 687, 708, 717
DpnII GATC 6 cut(s) 421, 613, 666, 685, 706, 715
DriI GACNNNNNGTC 1 cut(s) 404
Eam1104I CTCTTC 2 cut(s) 596, 774
Eam1105I GACNNNNNGTC 1 cut(s) 404
EarI CTCTTC 2 cut(s) 596, 774
Eco130I CCWWGG 1 cut(s) 907
Eco47I GGWCC 2 cut(s) 229, 660
Eco81I CCTNAGG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 660
EcoRI GAATTC 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 907
ErhI CCWWGG 1 cut(s) 907
FaeI CATG 5 cut(s) 138, 203, 440, 600, 635
FalI AAGNNNNNCTT 4 cut(s) 702, 734, 887, 919
FaqI GGGAC 2 cut(s) 236, 673
FatI CATG 5 cut(s) 134, 199, 436, 596, 631
FauI CCCGC 1 cut(s) 115
FauNDI CATATG 2 cut(s) 342, 975
FbaI TGATCA 2 cut(s) 421, 613
Fnu4HI GCNGC 3 cut(s) 68, 428, 741
FokI GGATG 3 cut(s) 23, 742, 803
Fsp4HI GCNGC 3 cut(s) 68, 428, 741
FspBI CTAG 2 cut(s) 33, 683
GlaI GCGC 1 cut(s) 744
GluI GCNGC 3 cut(s) 68, 428, 741
HaeIII GGCC 1 cut(s) 281
HapII CCGG 2 cut(s) 254, 757
HhaI GCGC 1 cut(s) 745
Hin1II CATG 5 cut(s) 138, 203, 440, 600, 635
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 1 cut(s) 479
HpaII CCGG 2 cut(s) 254, 757
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 5 cut(s) 90, 229, 334, 470, 580
Hpy188III TCNNGA 3 cut(s) 652, 710, 757
Hpy8I GTNNAC 5 cut(s) 90, 229, 334, 470, 580
Hpy99I CGWCG 1 cut(s) 402
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 6 cut(s) 28, 314, 402, 502, 611, 842
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 794
HpyF3I CTNAG 3 cut(s) 474, 701, 808
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 5 cut(s) 138, 203, 440, 600, 635
HspAI GCGC 1 cut(s) 743
Kpn2I TCCGGA 1 cut(s) 756
Ksp22I TGATCA 2 cut(s) 421, 613
Kzo9I GATC 6 cut(s) 421, 613, 666, 685, 706, 715
LmnI GCTCC 3 cut(s) 355, 467, 750
Lsp1109I GCAGC 2 cut(s) 439, 727
LweI GCATC 2 cut(s) 720, 781
MaeI CTAG 2 cut(s) 33, 683
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 1 cut(s) 605
MalI GATC 6 cut(s) 423, 615, 668, 687, 708, 717
MboI GATC 6 cut(s) 421, 613, 666, 685, 706, 715
MboII GAAGA 8 cut(s) 485, 573, 598, 613, 725, 791, 871, 952
MflI RGATCY 2 cut(s) 685, 715
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 8 cut(s) 48, 222, 354, 569, 620, 646, 693, 774
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 9 cut(s) 31, 113, 134, 214, 250, 469, 708, 775, 884
MroI TCCGGA 1 cut(s) 756
MroXI GAANNNNTTC 1 cut(s) 902
MseI TTAA 4 cut(s) 534, 572, 645, 997
MslI CAYNNNNRTG 1 cut(s) 133
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 2 cut(s) 254, 757
MspR9I CCNGG 2 cut(s) 64, 254
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 1 cut(s) 64
NciI CCSGG 1 cut(s) 254
NdeI CATATG 2 cut(s) 342, 975
NdeII GATC 6 cut(s) 421, 613, 666, 685, 706, 715
NlaIII CATG 5 cut(s) 138, 203, 440, 600, 635
NlaIV GGNNCC 2 cut(s) 166, 661
NmuCI GTSAC 1 cut(s) 605
NspI RCATGY 1 cut(s) 635
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 902
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 3 cut(s) 69, 429, 742
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 660
Psp1406I AACGTT 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 660
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 2 cut(s) 166, 661
PspPI GGNCC 2 cut(s) 229, 660
PspPPI RGGWCCY 1 cut(s) 660
PsuI RGATCY 2 cut(s) 685, 715
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 2 cut(s) 323, 449
RsaNI GTAC 2 cut(s) 322, 448
RseI CAYNNNNRTG 1 cut(s) 133
SaqAI TTAA 4 cut(s) 534, 572, 645, 997
SatI GCNGC 3 cut(s) 68, 428, 741
Sau3AI GATC 6 cut(s) 421, 613, 666, 685, 706, 715
Sau96I GGNCC 2 cut(s) 229, 660
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 2 cut(s) 64, 254
SduI GDGCHC 1 cut(s) 43
SfaNI GCATC 2 cut(s) 720, 781
SinI GGWCC 2 cut(s) 229, 660
SmiMI CAYNNNNRTG 1 cut(s) 133
Sse9I AATT 8 cut(s) 48, 222, 354, 569, 620, 646, 693, 774
SsiI CCGC 3 cut(s) 67, 108, 733
SspMI CTAG 2 cut(s) 33, 683
StyD4I CCNGG 2 cut(s) 62, 252
StyI CCWWGG 1 cut(s) 907
TaaI ACNGT 6 cut(s) 28, 314, 402, 502, 611, 842
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 935
TasI AATT 8 cut(s) 48, 222, 354, 569, 620, 646, 693, 774
TauI GCSGC 1 cut(s) 70
Tru1I TTAA 4 cut(s) 534, 572, 645, 997
Tru9I TTAA 4 cut(s) 534, 572, 645, 997
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 2 cut(s) 427, 740
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 6 cut(s) 163, 188, 289, 359, 937, 992
VpaK11BI GGWCC 2 cut(s) 229, 660
XapI RAATTY 2 cut(s) 620, 693
XceI RCATGY 1 cut(s) 635
XmnI GAANNNNTTC 1 cut(s) 902
XspI CTAG 2 cut(s) 33, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.