Rh5BG111800

ALA-interacting subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005969
Physical Location & Seq
Forward (+)
10462567 .. 10468677
6111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG111800.1

Sequence Viewer

Length: 1215 bp
ATGGAAGTGGATGGTGGAAGAATCTTAAAAGTATCTAGTGGTGCTCAATCTATACCACGACATTCCAGAAAACATAAAGCTTTCTATCAATTTACTCAGCAGAGTCTCCCTGCTTGTAAACCTGTCCTCACACCAGCATGGGTGATTACTACATTTTTATTGATGGGTGTCATTTTCATACCTATTGGGCTTATTACTCTTCATGCCTCTGAGAATGTTGTTGAAATAGTGGACCGATATGATACTGAATGTGTCCCAGAAGCATTCAGAAGCAATAAGGTGGCATATATCAAAGATAACTCGATTCCCAAAAACTGTTCTCGGTACTTGAAGGTCTACAAGCATATGAAAGCTCCAATAAATATCTACTATCAGCTTGATAACTACTTTCAGAACCACAGACGGTATGTCAAAAGTAAAAATGATCGACAGCTTTTACATGGATTGGGGTACAATGACACAGGCTCCTGTAAACCTGAGGAGTCCAACAATGGTCTTCCAATTGTTCCTTGTGGTTTAATAGCATGGAGTTTGTTTAATGATACATATACTCTTATTCGTGGAAGATCAGAGTTAAAGGTCAACAGGAAGAACATAGCATGGAAGAGTGACCGTGATCACAAGTTTGGGAAGAGTGTATATCCCTTCAATTTTCAGAACGGCACCTTGATGGGTGGTGGAAAGCTACACCCAAGAATCCCTCTAAGCGATCAAGAAGATCTTATTGTATGGATGCGGACAGCTGCACTCCCCAGTTTCCGTAAGTTGTATGGTAGAATTGAAGAGGATTTGAATGCAGATGATGTTGTGGTAGTCAATGTAACGAACAACTACAATACTTATAGTTTTGGAGGGAAAAAGAAGCTTATTCTTTCAACAACAGGCTGGTTAGGAGGAAAGAACAACTTCCTTGGGCTTGCCTGCACTTCTGTTGGTTCAAGTTCCATATTCATCTCTATTGTCTTCTTGCTGCTCCACATGAAAAATCCCAGAAAAATGGTGAAGGAGTTCAAAGGCATGCAAAACTGGGGAGAGGTTGCACCAGGAGCCCTGGTAACAACTCACAAAAAAGCTTCTAGTTTGCCTACACTAGAAACTATTATTGAAGAAGGATCAGAAGGCTTTGAAGTTTTGCCAAAGGGGGTCTTGGTATCTATTCTTCCTTTCCTCCTCTCCGGGGTTATGTACCTCATGATATGCAGAGATTTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

404

Amino Acids

45.45

Weight (kDa)

9.42

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 67 - 332 7.9e-81 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 662
AccI GTMKAC 1 cut(s) 336
AciI CCGC 1 cut(s) 736
AclWI GGATC 1 cut(s) 1120
AfaI GTAC 3 cut(s) 326, 452, 1187
AfiI CCNNNNNNNGG 1 cut(s) 1177
AhdI GACNNNNNGTC 1 cut(s) 407
AjnI CCWGG 2 cut(s) 1042, 1050
AluBI AGCT 8 cut(s) 80, 353, 376, 433, 685, 743, 865, 1073
AluI AGCT 8 cut(s) 80, 353, 376, 433, 685, 743, 865, 1073
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 1 cut(s) 1120
ApeKI GCWGC 2 cut(s) 743, 970
Asp700I GAANNNNTTC 2 cut(s) 905, 1007
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 1 cut(s) 1177
AsuHPI GGTGA 2 cut(s) 154, 1012
AvaII GGWCC 1 cut(s) 232
AxyI CCTNAGG 1 cut(s) 477
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 1051
BarI GAAGNNNNNNTAC 2 cut(s) 1143, 1175
BbsI GAAGAC 2 cut(s) 488, 955
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 2 cut(s) 730, 957
BccI CCATC 3 cut(s) 5, 157, 664
BceAI ACGGC 1 cut(s) 676
BciT130I CCWGG 2 cut(s) 1044, 1052
BclI TGATCA 1 cut(s) 616
BcnI CCSGG 1 cut(s) 1177
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 3 cut(s) 36, 1077, 1091
BglII AGATCT 1 cut(s) 718
BisI GCNGC 2 cut(s) 744, 971
BlsI GCNGC 2 cut(s) 745, 972
Bme1390I CCNGG 3 cut(s) 1044, 1052, 1177
Bme18I GGWCC 1 cut(s) 232
BmeRI GACNNNNNGTC 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 3 cut(s) 466, 664, 1048
BmrFI CCNGG 3 cut(s) 1044, 1052, 1177
BmrI ACTGGG 2 cut(s) 747, 1036
BmsI GCATC 1 cut(s) 723
BmuI ACTGGG 2 cut(s) 747, 1036
BpiI GAAGAC 2 cut(s) 488, 955
BpuMI CCSGG 1 cut(s) 1177
BsaJI CCNNGG 3 cut(s) 910, 1050, 1176
BsaXI ACNNNNNCTCC 4 cut(s) 449, 479, 1038, 1068
Bsc4I CCNNNNNNNGG 1 cut(s) 1177
Bse1I ACTGG 2 cut(s) 753, 1031
Bse21I CCTNAGG 1 cut(s) 477
BseBI CCWGG 2 cut(s) 1044, 1052
BseDI CCNNGG 3 cut(s) 910, 1050, 1176
BseGI GGATG 2 cut(s) 16, 738
BseLI CCNNNNNNNGG 1 cut(s) 1177
BseMII CTCAG 3 cut(s) 110, 201, 468
BseNI ACTGG 2 cut(s) 753, 1031
BseRI GAGGAG 2 cut(s) 494, 1160
BseXI GCAGC 2 cut(s) 730, 957
BsgI GTGCAG 2 cut(s) 729, 907
BshNI GGYRCC 1 cut(s) 662
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 1 cut(s) 1176
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 1177
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 2 cut(s) 263, 799
Bsp1286I GDGCHC 2 cut(s) 46, 1051
Bsp143I GATC 6 cut(s) 424, 566, 616, 709, 718, 1112
BspACI CCGC 1 cut(s) 736
BspCNI CTCAG 3 cut(s) 109, 202, 469
BspHI TCATGA 1 cut(s) 1191
BspLI GGNNCC 3 cut(s) 466, 664, 1048
BspPI GGATC 1 cut(s) 1120
BspT107I GGYRCC 1 cut(s) 662
BsrI ACTGG 2 cut(s) 753, 1031
BssECI CCNNGG 3 cut(s) 910, 1050, 1176
BssMI GATC 6 cut(s) 424, 566, 616, 709, 718, 1112
BssT1I CCWWGG 1 cut(s) 910
Bst2UI CCWGG 2 cut(s) 1044, 1052
Bst4CI ACNGT 3 cut(s) 317, 405, 614
Bst6I CTCTTC 4 cut(s) 204, 599, 626, 777
BstC8I GCNNGC 3 cut(s) 918, 922, 1019
BstDEI CTNAG 4 cut(s) 96, 210, 477, 704
BstF5I GGATG 2 cut(s) 16, 738
BstKTI GATC 6 cut(s) 427, 569, 619, 712, 721, 1115
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 6 cut(s) 424, 566, 616, 709, 718, 1112
BstMWI GCNNNNNNNGC 1 cut(s) 1046
BstNI CCWGG 2 cut(s) 1044, 1052
BstNSI RCATGY 1 cut(s) 1021
BstSCI CCNGG 3 cut(s) 1042, 1050, 1175
BstV1I GCAGC 2 cut(s) 730, 957
BstV2I GAAGAC 2 cut(s) 488, 955
BstX2I RGATCY 1 cut(s) 718
BstXI CCANNNNNNTGG 1 cut(s) 997
BstYI RGATCY 1 cut(s) 718
Bsu36I CCTNAGG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 16, 738
Cac8I GCNNGC 3 cut(s) 918, 922, 1019
CciI TCATGA 1 cut(s) 1191
Cfr13I GGNCC 1 cut(s) 232
Csp6I GTAC 3 cut(s) 325, 451, 1186
CviAII CATG 9 cut(s) 138, 203, 440, 525, 600, 979, 1018, 1192, 1212
CviQI GTAC 3 cut(s) 325, 451, 1186
DdeI CTNAG 4 cut(s) 96, 210, 477, 704
DpnI GATC 6 cut(s) 426, 568, 618, 711, 720, 1114
DpnII GATC 6 cut(s) 424, 566, 616, 709, 718, 1112
DriI GACNNNNNGTC 1 cut(s) 407
Eam1104I CTCTTC 4 cut(s) 204, 599, 626, 777
Eam1105I GACNNNNNGTC 1 cut(s) 407
EarI CTCTTC 4 cut(s) 204, 599, 626, 777
Eco130I CCWWGG 1 cut(s) 910
Eco24I GRGCYC 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 232
Eco81I CCTNAGG 1 cut(s) 477
EcoRII CCWGG 2 cut(s) 1042, 1050
EcoT14I CCWWGG 1 cut(s) 910
EcoT38I GRGCYC 1 cut(s) 1051
ErhI CCWWGG 1 cut(s) 910
FaeI CATG 9 cut(s) 141, 206, 443, 528, 603, 982, 1021, 1195, 1215
FalI AAGNNNNNCTT 6 cut(s) 705, 737, 890, 922, 1130, 1162
FaqI GGGAC 1 cut(s) 239
FatI CATG 9 cut(s) 137, 202, 439, 524, 599, 978, 1017, 1191, 1211
FauNDI CATATG 1 cut(s) 345
FbaI TGATCA 1 cut(s) 616
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 744, 971
FokI GGATG 2 cut(s) 23, 745
FriOI GRGCYC 1 cut(s) 1051
Fsp4HI GCNGC 2 cut(s) 744, 971
FspBI CTAG 3 cut(s) 36, 1077, 1091
GluI GCNGC 2 cut(s) 744, 971
HapII CCGG 1 cut(s) 1176
Hin1II CATG 9 cut(s) 141, 206, 443, 528, 603, 982, 1021, 1195, 1215
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HindIII AAGCTT 3 cut(s) 78, 863, 1071
HinfI GANTC 5 cut(s) 21, 103, 304, 482, 696
HpaII CCGG 1 cut(s) 1176
HphI GGTGA 2 cut(s) 154, 1012
Hpy166II GTNNAC 5 cut(s) 119, 232, 337, 473, 583
Hpy188I TCNGA 6 cut(s) 211, 269, 393, 571, 657, 1117
Hpy188III TCNNGA 3 cut(s) 66, 713, 1192
Hpy8I GTNNAC 5 cut(s) 119, 232, 337, 473, 583
HpyAV CCTTC 5 cut(s) 325, 655, 997, 1103, 1112
HpyCH4III ACNGT 3 cut(s) 317, 405, 614
HpyCH4V TGCA 6 cut(s) 746, 797, 924, 1021, 1040, 1200
HpyF10VI GCNNNNNNNGC 1 cut(s) 1046
HpyF3I CTNAG 4 cut(s) 96, 210, 477, 704
Hsp92II CATG 9 cut(s) 141, 206, 443, 528, 603, 982, 1021, 1195, 1215
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 6 cut(s) 424, 566, 616, 709, 718, 1112
LmnI GCTCC 4 cut(s) 358, 470, 978, 1046
Lsp1109I GCAGC 2 cut(s) 730, 957
LweI GCATC 1 cut(s) 723
MaeI CTAG 3 cut(s) 36, 1077, 1091
MaeIII GTNAC 3 cut(s) 608, 820, 1054
MalI GATC 6 cut(s) 426, 568, 618, 711, 720, 1114
MboI GATC 6 cut(s) 424, 566, 616, 709, 718, 1112
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 2 cut(s) 46, 1051
MluCI AATT 4 cut(s) 89, 501, 649, 777
MlyI GAGTC 2 cut(s) 112, 491
MmeI TCCRAC 1 cut(s) 510
MroXI GAANNNNTTC 2 cut(s) 905, 1007
MseI TTAA 4 cut(s) 26, 518, 537, 575
MslI CAYNNNNRTG 2 cut(s) 136, 668
MspA1I CMGCKG 1 cut(s) 743
MspI CCGG 1 cut(s) 1176
MspR9I CCNGG 3 cut(s) 1044, 1052, 1177
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 2 cut(s) 263, 799
MvaI CCWGG 2 cut(s) 1044, 1052
MwoI GCNNNNNNNGC 1 cut(s) 1046
NciI CCSGG 1 cut(s) 1177
NdeI CATATG 1 cut(s) 345
NdeII GATC 6 cut(s) 424, 566, 616, 709, 718, 1112
NlaIII CATG 9 cut(s) 141, 206, 443, 528, 603, 982, 1021, 1195, 1215
NlaIV GGNNCC 3 cut(s) 466, 664, 1048
NmuCI GTSAC 1 cut(s) 608
NspI RCATGY 1 cut(s) 1021
PaeI GCATGC 1 cut(s) 1021
PagI TCATGA 1 cut(s) 1191
PctI GAATGC 2 cut(s) 263, 799
PdmI GAANNNNTTC 2 cut(s) 905, 1007
PfeI GAWTC 3 cut(s) 21, 304, 696
PkrI GCNGC 2 cut(s) 745, 972
PleI GAGTC 2 cut(s) 111, 490
PpsI GAGTC 2 cut(s) 111, 490
Psp6I CCWGG 2 cut(s) 1042, 1050
PspGI CCWGG 2 cut(s) 1042, 1050
PspN4I GGNNCC 3 cut(s) 466, 664, 1048
PspPI GGNCC 1 cut(s) 232
PsuI RGATCY 1 cut(s) 718
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 3 cut(s) 326, 452, 1187
RsaNI GTAC 3 cut(s) 325, 451, 1186
RseI CAYNNNNRTG 2 cut(s) 136, 668
SaqAI TTAA 4 cut(s) 26, 518, 537, 575
SatI GCNGC 2 cut(s) 744, 971
Sau3AI GATC 6 cut(s) 424, 566, 616, 709, 718, 1112
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 2 cut(s) 112, 491
ScrFI CCNGG 3 cut(s) 1044, 1052, 1177
SduI GDGCHC 2 cut(s) 46, 1051
SfaNI GCATC 1 cut(s) 723
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 136, 668
SphI GCATGC 1 cut(s) 1021
Sse9I AATT 4 cut(s) 89, 501, 649, 777
SsiI CCGC 1 cut(s) 736
SspMI CTAG 3 cut(s) 36, 1077, 1091
StyD4I CCNGG 3 cut(s) 1042, 1050, 1175
StyI CCWWGG 1 cut(s) 910
TaaI ACNGT 3 cut(s) 317, 405, 614
TaqI TCGA 2 cut(s) 302, 427
TaqII GACCGA 1 cut(s) 249
TasI AATT 4 cut(s) 89, 501, 649, 777
TfiI GAWTC 3 cut(s) 21, 304, 696
Tru1I TTAA 4 cut(s) 26, 518, 537, 575
Tru9I TTAA 4 cut(s) 26, 518, 537, 575
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 2 cut(s) 743, 970
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 5 cut(s) 166, 191, 362, 940, 995
TspGWI ACGGA 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 232
XceI RCATGY 1 cut(s) 1021
XmiI GTMKAC 1 cut(s) 336
XmnI GAANNNNTTC 2 cut(s) 905, 1007
XspI CTAG 3 cut(s) 36, 1077, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.