MD05G1280600.v1.1

ALA-interacting subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005969
Physical Location & Seq
Reverse (-)
41466115 .. 41471726
5612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1280600.v1.1.491

Sequence Viewer

Length: 999 bp
ATGGAAGTGGATGGAAGTGCCTCAACAGTTTCTAGGGGTGCTCAATCAATTCCACGGCAAACCAGGCGACAGAAGGCTTTTTATCAATTTCAACAGCAGAGTCTACCGGCTTGTAAACCCGTCCTCACACCAGCATGGGTGATTACCACATTTTTATTGATCGGGGCCATTTTCATACCTGCGGGGCTTATTACTCTTAACGTCTCCCAGAATGTTGTGGAAATTGTGGACCAATATGATGCGGAATGTGTACCAGAGGCATTCAAGAGTAACAAGGTGGCATATATCAAAGATAACTTAATTCCTAAAAACTGTTCTCGGTACTTGAAGGTTTACAAGGACATGAAAGCTCCAATTTATATCTACTACCAGCTTGATAACTACTATCAAAACCACCGACGGTATGTCAAAAGTAGAAGTGATCAGCAGCTTTTACATGGATTGGGGCACAATGACACAAGCTCCTGTCAACCTGAGGAGTCCAACAAGGATCTTCCCATTGTTCCTTGTGGCTTGATAGCATGGAGTTTGTTTAATGATACATATACTTTTATTCGCGGAAGATCAGAATTAAAAGTCAACAGGAAGGACATAGCTTGGAAGAGCGACCGTGATCACAAATTTGGGAAGCATGTATATCCCTTTAATTTCCAGAACGGGACCTTGATTGGTGGTGCAAAGCTAGATCCAAAAATTCCTCTAAGCGATCAAGAAGACCTTATTGTATGGATGCGGACAGCTGCGCTCCCCAGTTTCCGGAAGCTGTACGGTAGAATTGAAGAGGATTTGGATGCAGATGATGTTCTAGTAGTGAATCTAACGAACAACTACAATACTTACAGTTTTGGTGGAAAAAAGAAGCTTATCCTTTCAACATCAAGTTGGTTAGGAGGAAAGAATGACTTCCTTGGACTTGCCTACACTTTTGTTGGTTCAACTTCCATATTCATTTCTGTTGTCTTCTTGTTGCTGCATGTGAAATATCCAAGAACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC50

Protein Analysis

333

Amino Acids

37.79

Weight (kDa)

9.14

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC50 PF03381 67 - 330 9.1e-85 LEM3 (ligand-effect modulator 3) family / CDC50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 103
AccII CGCG 1 cut(s) 558
AccIII TCCGGA 1 cut(s) 756
AciI CCGC 4 cut(s) 182, 242, 558, 733
AclWI GGATC 2 cut(s) 498, 680
AcsI RAATTY 2 cut(s) 620, 693
AfaI GTAC 3 cut(s) 252, 323, 767
AfiI CCNNNNNNNGG 1 cut(s) 756
AgsI TTSAA 6 cut(s) 92, 265, 328, 779, 873, 936
AhdI GACNNNNNGTC 1 cut(s) 404
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 9 cut(s) 350, 373, 430, 462, 596, 682, 740, 763, 862
AluI AGCT 9 cut(s) 350, 373, 430, 462, 596, 682, 740, 763, 862
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 1 cut(s) 208
AlwI GGATC 2 cut(s) 498, 680
Aor13HI TCCGGA 1 cut(s) 756
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 3 cut(s) 427, 740, 970
ApoI RAATTY 2 cut(s) 620, 693
Asp700I GAANNNNTTC 1 cut(s) 902
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 3 cut(s) 165, 229, 660
AsuHPI GGTGA 1 cut(s) 151
AvaII GGWCC 2 cut(s) 229, 660
AxyI CCTNAGG 1 cut(s) 474
BaeGI GKGCMC 1 cut(s) 450
BbsI GAAGAC 2 cut(s) 720, 952
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 3 cut(s) 439, 727, 957
BccI CCATC 1 cut(s) 5
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 64
BclI TGATCA 2 cut(s) 421, 613
BcoDI GTCTC 1 cut(s) 208
BfaI CTAG 3 cut(s) 33, 683, 806
BfuAI ACCTGC 1 cut(s) 187
BisI GCNGC 3 cut(s) 428, 741, 971
BlsI GCNGC 3 cut(s) 429, 742, 972
Bme1390I CCNGG 1 cut(s) 64
Bme18I GGWCC 2 cut(s) 229, 660
BmeRI GACNNNNNGTC 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 165, 229, 660
BmiI GGNNCC 2 cut(s) 166, 661
BmrFI CCNGG 1 cut(s) 64
BmrI ACTGGG 1 cut(s) 744
BmsI GCATC 3 cut(s) 229, 720, 781
BmuI ACTGGG 1 cut(s) 744
BpiI GAAGAC 2 cut(s) 720, 952
BsaJI CCNNGG 2 cut(s) 53, 907
BsaWI WCCGGW 1 cut(s) 756
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 756
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 750
Bse21I CCTNAGG 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 756
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 53, 907
BseGI GGATG 3 cut(s) 16, 735, 796
BseLI CCNNNNNNNGG 1 cut(s) 756
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 1 cut(s) 750
BseRI GAGGAG 1 cut(s) 491
BseSI GKGCMC 1 cut(s) 450
BseXI GCAGC 3 cut(s) 439, 727, 957
Bsh1236I CGCG 1 cut(s) 558
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 2 cut(s) 107, 757
BslFI GGGAC 1 cut(s) 673
BslI CCNNNNNNNGG 1 cut(s) 756
BsmAI GTCTC 1 cut(s) 208
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 673
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 43, 450
Bsp13I TCCGGA 1 cut(s) 756
Bsp143I GATC 7 cut(s) 159, 421, 490, 563, 613, 685, 706
BspACI CCGC 4 cut(s) 182, 242, 558, 733
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 1 cut(s) 466
BspEI TCCGGA 1 cut(s) 756
BspFNI CGCG 1 cut(s) 558
BspLI GGNNCC 2 cut(s) 166, 661
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 2 cut(s) 498, 680
BspQI GCTCTTC 1 cut(s) 596
BsrFI RCCGGY 1 cut(s) 106
BsrI ACTGG 1 cut(s) 750
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 2 cut(s) 53, 907
BssMI GATC 7 cut(s) 159, 421, 490, 563, 613, 685, 706
BssT1I CCWWGG 1 cut(s) 907
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 6 cut(s) 28, 314, 402, 611, 770, 842
Bst6I CTCTTC 2 cut(s) 596, 774
BstDEI CTNAG 2 cut(s) 474, 701
BstDSI CCRYGG 1 cut(s) 53
BstF5I GGATG 3 cut(s) 16, 735, 796
BstFNI CGCG 1 cut(s) 558
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 7 cut(s) 162, 424, 493, 566, 616, 688, 709
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 7 cut(s) 159, 421, 490, 563, 613, 685, 706
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 2 cut(s) 635, 977
BstSCI CCNGG 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 450
BstUI CGCG 1 cut(s) 558
BstV1I GCAGC 3 cut(s) 439, 727, 957
BstV2I GAAGAC 2 cut(s) 720, 952
BstX2I RGATCY 2 cut(s) 490, 685
BstYI RGATCY 2 cut(s) 490, 685
Bsu36I CCTNAGG 1 cut(s) 474
BsuRI GGCC 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 53
BtsCI GGATG 3 cut(s) 16, 735, 796
BveI ACCTGC 1 cut(s) 187
CfoI GCGC 1 cut(s) 745
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 165, 229, 660
Csp6I GTAC 3 cut(s) 251, 322, 766
CviAII CATG 6 cut(s) 135, 343, 437, 522, 632, 974
CviQI GTAC 3 cut(s) 251, 322, 766
DdeI CTNAG 2 cut(s) 474, 701
DpnI GATC 7 cut(s) 161, 423, 492, 565, 615, 687, 708
DpnII GATC 7 cut(s) 159, 421, 490, 563, 613, 685, 706
DriI GACNNNNNGTC 1 cut(s) 404
Eam1104I CTCTTC 2 cut(s) 596, 774
Eam1105I GACNNNNNGTC 1 cut(s) 404
EarI CTCTTC 2 cut(s) 596, 774
Eco130I CCWWGG 1 cut(s) 907
Eco47I GGWCC 2 cut(s) 229, 660
Eco81I CCTNAGG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 660
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 907
ErhI CCWWGG 1 cut(s) 907
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 6 cut(s) 138, 346, 440, 525, 635, 977
FalI AAGNNNNNCTT 4 cut(s) 702, 734, 887, 919
FaqI GGGAC 1 cut(s) 673
FatI CATG 6 cut(s) 134, 342, 436, 521, 631, 973
FauI CCCGC 1 cut(s) 175
FbaI TGATCA 2 cut(s) 421, 613
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 428, 741, 971
FokI GGATG 3 cut(s) 23, 742, 803
Fsp4HI GCNGC 3 cut(s) 428, 741, 971
FspBI CTAG 3 cut(s) 33, 683, 806
GlaI GCGC 1 cut(s) 744
GluI GCNGC 3 cut(s) 428, 741, 971
HaeIII GGCC 1 cut(s) 167
HapII CCGG 2 cut(s) 107, 757
HhaI GCGC 1 cut(s) 745
Hin1II CATG 6 cut(s) 138, 346, 440, 525, 635, 977
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HincII GTYRAC 2 cut(s) 470, 580
HindII GTYRAC 2 cut(s) 470, 580
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 3 cut(s) 100, 479, 814
HpaII CCGG 2 cut(s) 107, 757
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 7 cut(s) 104, 116, 229, 251, 334, 470, 580
Hpy188I TCNGA 1 cut(s) 568
Hpy188III TCNNGA 4 cut(s) 265, 652, 710, 757
Hpy8I GTNNAC 7 cut(s) 104, 116, 229, 251, 334, 470, 580
Hpy99I CGWCG 1 cut(s) 402
HpyAV CCTTC 3 cut(s) 67, 322, 580
HpyCH4III ACNGT 6 cut(s) 28, 314, 402, 611, 770, 842
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 3 cut(s) 677, 794, 973
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpyF3I CTNAG 2 cut(s) 474, 701
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 6 cut(s) 138, 346, 440, 525, 635, 977
HspAI GCGC 1 cut(s) 743
Kpn2I TCCGGA 1 cut(s) 756
Ksp22I TGATCA 2 cut(s) 421, 613
Kzo9I GATC 7 cut(s) 159, 421, 490, 563, 613, 685, 706
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 3 cut(s) 355, 467, 750
Lsp1109I GCAGC 3 cut(s) 439, 727, 957
LweI GCATC 3 cut(s) 229, 720, 781
MaeI CTAG 3 cut(s) 33, 683, 806
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 269
MalI GATC 7 cut(s) 161, 423, 492, 565, 615, 687, 708
MboI GATC 7 cut(s) 159, 421, 490, 563, 613, 685, 706
MboII GAAGA 6 cut(s) 485, 573, 613, 725, 791, 952
MflI RGATCY 2 cut(s) 490, 685
MhlI GDGCHC 2 cut(s) 43, 450
MlyI GAGTC 2 cut(s) 109, 488
MmeI TCCRAC 1 cut(s) 507
MnlI CCTC 7 cut(s) 31, 134, 250, 469, 708, 775, 884
MroI TCCGGA 1 cut(s) 756
MroXI GAANNNNTTC 1 cut(s) 902
MseI TTAA 6 cut(s) 198, 299, 534, 572, 645, 997
MslI CAYNNNNRTG 1 cut(s) 133
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 2 cut(s) 107, 757
MspR9I CCNGG 1 cut(s) 64
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 1 cut(s) 64
MvnI CGCG 1 cut(s) 558
MwoI GCNNNNNNNGC 1 cut(s) 64
NdeII GATC 7 cut(s) 159, 421, 490, 563, 613, 685, 706
NlaIII CATG 6 cut(s) 138, 346, 440, 525, 635, 977
NlaIV GGNNCC 2 cut(s) 166, 661
NspI RCATGY 2 cut(s) 635, 977
PciSI GCTCTTC 1 cut(s) 596
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 902
PfeI GAWTC 1 cut(s) 814
PkrI GCNGC 3 cut(s) 429, 742, 972
PleI GAGTC 2 cut(s) 108, 487
PpsI GAGTC 2 cut(s) 108, 487
PpuMI RGGWCCY 1 cut(s) 660
Psp5II RGGWCCY 1 cut(s) 660
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 2 cut(s) 166, 661
PspPI GGNCC 3 cut(s) 165, 229, 660
PspPPI RGGWCCY 1 cut(s) 660
PsuI RGATCY 2 cut(s) 490, 685
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 3 cut(s) 252, 323, 767
RsaNI GTAC 3 cut(s) 251, 322, 766
RseI CAYNNNNRTG 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 596
SaqAI TTAA 6 cut(s) 198, 299, 534, 572, 645, 997
SatI GCNGC 3 cut(s) 428, 741, 971
Sau3AI GATC 7 cut(s) 159, 421, 490, 563, 613, 685, 706
Sau96I GGNCC 3 cut(s) 165, 229, 660
SchI GAGTC 2 cut(s) 109, 488
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 2 cut(s) 43, 450
SfaNI GCATC 3 cut(s) 229, 720, 781
SinI GGWCC 2 cut(s) 229, 660
SmiMI CAYNNNNRTG 1 cut(s) 133
SsiI CCGC 4 cut(s) 182, 242, 558, 733
SspMI CTAG 3 cut(s) 33, 683, 806
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 907
TaaI ACNGT 6 cut(s) 28, 314, 402, 611, 770, 842
TaiI ACGT 1 cut(s) 204
TfiI GAWTC 1 cut(s) 814
Tru1I TTAA 6 cut(s) 198, 299, 534, 572, 645, 997
Tru9I TTAA 6 cut(s) 198, 299, 534, 572, 645, 997
TseI GCWGC 3 cut(s) 427, 740, 970
TspDTI ATGAA 3 cut(s) 163, 359, 937
VpaK11BI GGWCC 2 cut(s) 229, 660
XapI RAATTY 2 cut(s) 620, 693
XceI RCATGY 2 cut(s) 635, 977
XmiI GTMKAC 1 cut(s) 103
XmnI GAANNNNTTC 1 cut(s) 902
XspI CTAG 3 cut(s) 33, 683, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.