AT5G46680

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012545
Physical Location & Seq
Forward (+)
18940903 .. 18942672
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46680.1

Sequence Viewer

Length: 1407 bp
ATGGTTCGTGGATTGATGAAATTCCCAGGAATCTCAACGAAGTTATTAAACATAAGCGTTAATTCTCTCTGCAAATTTCGAAACTTGGAGAGAGCAGAAACATTACTCATAGACGGAATCAGATTAGGTGTTCTTCCTGATGTGATCACTTACAACACTCTAATCAAAGGGTACACTCGTTTCATTGGAATCGACGAAGCTTATGCTGTAACACGTCGTATGAGAGAAGCTGGGATCGAACCAGATGTTACTACTTACAATTCATTGATCTCAGGAGCTGCTAAAAACTTGATGTTGAATCGTGTACTCCAACTGTTCGACGAAATGCTTCACTCAGGTTTGTCTCCTGATATGTGGAGTTATAATACTTTAATGTCTTGTTACTTCAAATTAGGGAGACATGGAGAAGCGTTTAAGATTCTTCATGAGGATATTCATTTAGCTGGGTTGGTTCCTGGGATTGATACTTATAACATTCTGCTTGATGCGCTTTGTAAAAGCGGTCATACGGATAATGCGATTGAGTTGTTTAAGCATCTGAAGAGTCGGGTTAAACCCGAGCTTATGACGTATAATATTCTCATCAATGGTCTTTGTAAATCGAGAAGGGTTGGTTCTGTGGATTGGATGATGAGAGAGCTTAAGAAATCGGGCTATACTCCGAATGCGGTTACGTATACAACGATGCTTAAAATGTATTTTAAGACTAAGAGGATTGAGAAAGGTCTTCAGTTGTTTTTGAAGATGAAGAAAGAAGGGTATACGTTTGACGGGTTTGCGAATTGTGCGGTTGTTAGTGCGTTGATTAAAACCGGAAGAGCAGAGGAGGCGTATGAGTGTATGCACGAGCTTGTGAGAAGTGGTACAAGGAGTCAAGACATTGTTTCGTACAATACATTGTTGAATTTGTATTTTAAAGATGGGAACTTGGATGCAGTAGATGATCTATTGGAGGAGATTGAGATGAAGGGGTTGAAACCTGATGACTATACTCATACGATTATAGTCAATGGTTTGTTAAATATAGGCAATACGGGAGGAGCCGAGAAGCATTTGGCATGTATCGGAGAGATGGGGATGCAGCCGAGTGTTGTCACTTGTAACTGTTTGATTGATGGGTTGTGCAAAGCAGGTCATGTAGATCGTGCAATGAGGTTGTTTGCGTCAATGGAAGTTAGAGATGAGTTCACTTACACTTCTGTTGTGCATAATCTATGCAAAGACGGACGGCTTGTTTGTGCTTCGAAGCTGCTCTTGTCGTGTTACAATAAAGGAATGAAGATTCCGTCATCGGCTAGACGAGCAGTGTTGTCCGGCATACGGGAGACGGTATCTTACCAAGCAGCAAGGAAGACACATATCAAAATCAAAGCAGCTATTGAGTGTAATACACTAATGTACCCTTAA

Protein Analysis

468

Amino Acids

52.51

Weight (kDa)

8.89

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 41 - 91 8.2e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 43 - 75 1.2e-06 PPR repeat
PPR_2 PF13041 46 - 95 2.4e-13 PPR repeat family
PPR_1 PF12854 78 - 109 2.3e-06 PPR repeat
PPR_2 PF13041 81 - 125 1.1e-11 PPR repeat family
PPR PF01535 85 - 114 5e-06 PPR repeat
PPR_3 PF13812 105 - 164 9.9e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 113 - 143 8.6e-06 PPR repeat
PPR_3 PF13812 143 - 196 9.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 149 - 179 2e-08 PPR repeat
PPR_2 PF13041 152 - 200 1.7e-13 PPR repeat family
PPR PF01535 156 - 182 5.5e-06 PPR repeat
PPR_1 PF12854 184 - 204 7.7e-06 PPR repeat
PPR_2 PF13041 188 - 235 5.6e-12 PPR repeat family
PPR_2 PF13041 221 - 257 4.7e-08 PPR repeat family
PPR PF01535 224 - 253 2e-06 PPR repeat
PPR_2 PF13041 293 - 340 5.1e-14 PPR repeat family
PPR_1 PF12854 358 - 391 7.5e-12 PPR repeat
PPR_2 PF13041 362 - 407 3.4e-12 PPR repeat family
PPR PF01535 365 - 392 8.5e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012545)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46680
fragaria_vesca FvH4_6g27690
malus_domestica MD07G1055100.v1.1 MD15G1427800.v1.1
prunus_persica Prupe.3G114500_v2.0.a1 Prupe.3G114500_v2.0.a1 Prupe.4G280300_v2.0.a1
pyrus_communis pycom07g04330
rosa_chinensis RchiOBHm_Chr2g0130841
rosa_laevigata RLG00000019152
rosa_multiflora Rmu_sc0003629.1_g000016
rosa_roxburghii Rroxscaffold_2G00113500 Rroxscaffold_4G00318090
rosa_rugosa Rorug02G0293500 Rorug02G0293600
rosa_samantha Rh2AG344900 Rh2BG353300 Rh2DG371200
rosa_wichuraiana Rw2G027470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 363, 471
Acc36I ACCTGC 1 cut(s) 1121
AccI GTMKAC 2 cut(s) 677, 761
AciI CCGC 3 cut(s) 501, 668, 788
AclWI GGATC 1 cut(s) 242
AcsI RAATTY 3 cut(s) 20, 74, 904
AcuI CTGAAG 2 cut(s) 560, 713
AfaI GTAC 5 cut(s) 173, 306, 865, 890, 1400
AfiI CCNNNNNNNGG 1 cut(s) 1320
AflII CTTAAG 1 cut(s) 641
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 5 cut(s) 298, 388, 742, 904, 976
AjiI CACGTC 1 cut(s) 215
AjnI CCWGG 2 cut(s) 25, 454
AluBI AGCT 9 cut(s) 200, 230, 278, 443, 562, 640, 850, 1249, 1376
AluI AGCT 9 cut(s) 200, 230, 278, 443, 562, 640, 850, 1249, 1376
Alw26I GTCTC 3 cut(s) 348, 391, 1319
AlwI GGATC 1 cut(s) 242
AlwNI CAGNNNCTG 1 cut(s) 278
Ama87I CYCGRG 1 cut(s) 557
ApeKI GCWGC 5 cut(s) 278, 1081, 1249, 1343, 1373
ApoI RAATTY 3 cut(s) 20, 74, 904
ArsI GACNNNNNNTTYG 2 cut(s) 1218, 1250
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 490
AsuII TTCGAA 2 cut(s) 79, 1244
AvaI CYCGRG 1 cut(s) 557
BaeI ACNNNNGTAYC 3 cut(s) 163, 196, 1382
BauI CACGAG 1 cut(s) 845
BbsI GAAGAC 2 cut(s) 719, 1358
BbvI GCAGC 5 cut(s) 265, 1093, 1236, 1355, 1385
BccI CCATC 3 cut(s) 914, 1066, 1109
BceAI ACGGC 1 cut(s) 1244
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 2 cut(s) 27, 456
BclI TGATCA 1 cut(s) 144
BcoDI GTCTC 3 cut(s) 348, 391, 1319
BfaI CTAG 1 cut(s) 1296
BfrI CTTAAG 1 cut(s) 641
BfuAI ACCTGC 1 cut(s) 1121
BisI GCNGC 5 cut(s) 279, 1082, 1250, 1344, 1374
BlsI GCNGC 5 cut(s) 280, 1083, 1251, 1345, 1375
Bme1390I CCNGG 2 cut(s) 27, 456
BmeT110I CYCGRG 1 cut(s) 557
BmgBI CACGTC 1 cut(s) 215
BmiI GGNNCC 2 cut(s) 453, 1042
BmrFI CCNGG 2 cut(s) 27, 456
BmsI GCATC 5 cut(s) 475, 544, 675, 922, 1068
BpiI GAAGAC 2 cut(s) 719, 1358
Bpu14I TTCGAA 2 cut(s) 79, 1244
BsaAI YACGTR 1 cut(s) 675
BsaJI CCNNGG 2 cut(s) 25, 455
BsaWI WCCGGW 1 cut(s) 812
Bsc4I CCNNNNNNNGG 1 cut(s) 1320
Bse3DI GCAATG 1 cut(s) 1155
BseBI CCWGG 2 cut(s) 27, 456
BseDI CCNNGG 2 cut(s) 25, 455
BseGI GGATG 3 cut(s) 633, 937, 1083
BseLI CCNNNNNNNGG 1 cut(s) 1320
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 2 cut(s) 285, 348
BseRI GAGGAG 3 cut(s) 839, 968, 1053
BseXI GCAGC 5 cut(s) 265, 1093, 1236, 1355, 1385
BseYI CCCAGC 2 cut(s) 230, 443
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 2 cut(s) 813, 1314
BslI CCNNNNNNNGG 1 cut(s) 1320
BsmAI GTCTC 3 cut(s) 348, 391, 1319
BsmBI CGTCTC 1 cut(s) 1319
BsmI GAATGC 1 cut(s) 670
BsoBI CYCGRG 1 cut(s) 557
Bsp119I TTCGAA 2 cut(s) 79, 1244
Bsp143I GATC 5 cut(s) 144, 234, 267, 943, 1141
BspACI CCGC 3 cut(s) 501, 668, 788
BspCNI CTCAG 2 cut(s) 284, 347
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 2 cut(s) 453, 1042
BspMI ACCTGC 1 cut(s) 1121
BspPI GGATC 1 cut(s) 242
BspQI GCTCTTC 1 cut(s) 811
BspT104I TTCGAA 2 cut(s) 79, 1244
BspTI CTTAAG 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 1155
BssECI CCNNGG 2 cut(s) 25, 455
BssMI GATC 5 cut(s) 144, 234, 267, 943, 1141
BssNAI GTATAC 2 cut(s) 678, 762
BssSI CACGAG 1 cut(s) 845
Bst1107I GTATAC 2 cut(s) 678, 762
Bst2BI CACGAG 1 cut(s) 845
Bst2UI CCWGG 2 cut(s) 27, 456
Bst4CI ACNGT 3 cut(s) 315, 1106, 1330
Bst6I CTCTTC 2 cut(s) 536, 811
BstAFI CTTAAG 1 cut(s) 641
BstBAI YACGTR 1 cut(s) 675
BstBI TTCGAA 2 cut(s) 79, 1244
BstDEI CTNAG 3 cut(s) 271, 334, 708
BstF5I GGATG 3 cut(s) 633, 937, 1083
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 5 cut(s) 147, 237, 270, 946, 1144
BstMAI GTCTC 3 cut(s) 348, 391, 1319
BstMBI GATC 5 cut(s) 144, 234, 267, 943, 1141
BstMWI GCNNNNNNNGC 4 cut(s) 487, 785, 827, 1301
BstNI CCWGG 2 cut(s) 27, 456
BstNSI RCATGY 1 cut(s) 1062
BstSCI CCNGG 2 cut(s) 25, 454
BstSNI TACGTA 1 cut(s) 675
BstV1I GCAGC 5 cut(s) 265, 1093, 1236, 1355, 1385
BstV2I GAAGAC 2 cut(s) 719, 1358
BstZ17I GTATAC 2 cut(s) 678, 762
BtrI CACGTC 1 cut(s) 215
BtsCI GGATG 3 cut(s) 633, 937, 1083
BtsI GCAGTG 1 cut(s) 1311
BtsIMutI CAGTG 1 cut(s) 1311
BveI ACCTGC 1 cut(s) 1121
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 424
CfoI GCGC 1 cut(s) 490
CseI GACGC 1 cut(s) 1152
Csp6I GTAC 5 cut(s) 172, 305, 864, 889, 1399
CviAII CATG 4 cut(s) 401, 425, 1059, 1136
CviQI GTAC 5 cut(s) 172, 305, 864, 889, 1399
DdeI CTNAG 3 cut(s) 271, 334, 708
DpnI GATC 5 cut(s) 146, 236, 269, 945, 1143
DpnII GATC 5 cut(s) 144, 234, 267, 943, 1141
DraI TTTAAA 1 cut(s) 916
Eam1104I CTCTTC 2 cut(s) 536, 811
EarI CTCTTC 2 cut(s) 536, 811
Eco105I TACGTA 1 cut(s) 675
Eco57I CTGAAG 2 cut(s) 560, 713
Eco88I CYCGRG 1 cut(s) 557
EcoRII CCWGG 2 cut(s) 25, 454
Esp3I CGTCTC 1 cut(s) 1319
FaeI CATG 4 cut(s) 404, 428, 1062, 1139
FalI AAGNNNNNCTT 2 cut(s) 1238, 1270
FatI CATG 4 cut(s) 400, 424, 1058, 1135
FbaI TGATCA 1 cut(s) 144
FblI GTMKAC 2 cut(s) 677, 761
Fnu4HI GCNGC 5 cut(s) 279, 1082, 1250, 1344, 1374
FokI GGATG 3 cut(s) 640, 944, 1090
Fsp4HI GCNGC 5 cut(s) 279, 1082, 1250, 1344, 1374
FspBI CTAG 1 cut(s) 1296
GlaI GCGC 1 cut(s) 489
GluI GCNGC 5 cut(s) 279, 1082, 1250, 1344, 1374
GsaI CCCAGC 2 cut(s) 234, 447
HapII CCGG 2 cut(s) 813, 1314
HgaI GACGC 1 cut(s) 1152
HhaI GCGC 1 cut(s) 490
Hin1II CATG 4 cut(s) 404, 428, 1062, 1139
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 8 cut(s) 30, 117, 189, 298, 418, 544, 871, 1282
HpaII CCGG 2 cut(s) 813, 1314
Hpy166II GTNNAC 5 cut(s) 174, 305, 678, 762, 1188
Hpy188I TCNGA 4 cut(s) 122, 540, 663, 1067
Hpy188III TCNNGA 6 cut(s) 137, 273, 347, 425, 603, 875
Hpy8I GTNNAC 5 cut(s) 174, 305, 678, 762, 1188
Hpy99I CGWCG 3 cut(s) 197, 219, 323
HpyAV CCTTC 3 cut(s) 600, 749, 961
HpyCH4III ACNGT 3 cut(s) 315, 1106, 1330
HpyCH4IV ACGT 4 cut(s) 214, 569, 674, 764
HpyCH4V TGCA 8 cut(s) 72, 844, 935, 1081, 1125, 1148, 1207, 1218
HpyF10VI GCNNNNNNNGC 4 cut(s) 487, 785, 827, 1301
HpyF3I CTNAG 3 cut(s) 271, 334, 708
HpySE526I ACGT 4 cut(s) 214, 569, 674, 764
Hsp92II CATG 4 cut(s) 404, 428, 1062, 1139
HspAI GCGC 1 cut(s) 488
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 5 cut(s) 144, 234, 267, 943, 1141
LguI GCTCTTC 1 cut(s) 811
LmnI GCTCC 2 cut(s) 275, 1040
Lsp1109I GCAGC 5 cut(s) 265, 1093, 1236, 1355, 1385
LweI GCATC 5 cut(s) 475, 544, 675, 922, 1068
MaeI CTAG 1 cut(s) 1296
MaeII ACGT 4 cut(s) 214, 569, 674, 764
MaeIII GTNAC 7 cut(s) 208, 247, 380, 670, 1093, 1100, 1262
MalI GATC 5 cut(s) 146, 236, 269, 945, 1143
MboI GATC 5 cut(s) 144, 234, 267, 943, 1141
MboII GAAGA 9 cut(s) 125, 413, 553, 719, 754, 760, 828, 1291, 1363
MluCI AATT 7 cut(s) 20, 61, 74, 259, 389, 781, 904
MlyI GAGTC 2 cut(s) 553, 880
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 7 cut(s) 421, 705, 817, 820, 946, 1031, 1146
MroXI GAANNNNTTC 1 cut(s) 327
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 2 cut(s) 813, 1314
MspR9I CCNGG 2 cut(s) 27, 456
Mva1269I GAATGC 1 cut(s) 670
MvaI CCWGG 2 cut(s) 27, 456
MwoI GCNNNNNNNGC 4 cut(s) 487, 785, 827, 1301
NdeII GATC 5 cut(s) 144, 234, 267, 943, 1141
NlaIII CATG 4 cut(s) 404, 428, 1062, 1139
NlaIV GGNNCC 2 cut(s) 453, 1042
NmeAIII GCCGAG 2 cut(s) 1069, 1110
NmuCI GTSAC 1 cut(s) 1093
NspI RCATGY 1 cut(s) 1062
NspV TTCGAA 2 cut(s) 79, 1244
PagI TCATGA 1 cut(s) 424
PciSI GCTCTTC 1 cut(s) 811
PcsI WCGNNNNNNNCGW 2 cut(s) 515, 680
PctI GAATGC 1 cut(s) 670
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 6 cut(s) 30, 117, 189, 298, 418, 1282
PkrI GCNGC 5 cut(s) 280, 1083, 1251, 1345, 1375
PleI GAGTC 2 cut(s) 552, 879
PpsI GAGTC 2 cut(s) 552, 879
Ppu21I YACGTR 1 cut(s) 675
PsiI TTATAA 2 cut(s) 363, 471
Psp6I CCWGG 2 cut(s) 25, 454
PspFI CCCAGC 2 cut(s) 230, 443
PspGI CCWGG 2 cut(s) 25, 454
PspN4I GGNNCC 2 cut(s) 453, 1042
PstNI CAGNNNCTG 1 cut(s) 278
RsaI GTAC 5 cut(s) 173, 306, 865, 890, 1400
RsaNI GTAC 5 cut(s) 172, 305, 864, 889, 1399
SapI GCTCTTC 1 cut(s) 811
SatI GCNGC 5 cut(s) 279, 1082, 1250, 1344, 1374
Sau3AI GATC 5 cut(s) 144, 234, 267, 943, 1141
SchI GAGTC 2 cut(s) 553, 880
ScrFI CCNGG 2 cut(s) 27, 456
SfaNI GCATC 5 cut(s) 475, 544, 675, 922, 1068
SfuI TTCGAA 2 cut(s) 79, 1244
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
SnaBI TACGTA 1 cut(s) 675
Sse9I AATT 7 cut(s) 20, 61, 74, 259, 389, 781, 904
SsiI CCGC 3 cut(s) 501, 668, 788
SspI AATATT 1 cut(s) 577
SspMI CTAG 1 cut(s) 1296
StyD4I CCNGG 2 cut(s) 25, 454
TaaI ACNGT 3 cut(s) 315, 1106, 1330
TaiI ACGT 4 cut(s) 217, 572, 677, 767
TaqI TCGA 6 cut(s) 79, 192, 237, 318, 602, 1244
TasI AATT 7 cut(s) 20, 61, 74, 259, 389, 781, 904
TatI WGTACW 1 cut(s) 304
TfiI GAWTC 6 cut(s) 30, 117, 189, 298, 418, 1282
TscAI CASTG 1 cut(s) 1311
TseFI GTSAC 1 cut(s) 1093
TseI GCWGC 5 cut(s) 278, 1081, 1249, 1343, 1373
Tsp45I GTSAC 1 cut(s) 1093
TspDTI ATGAA 8 cut(s) 32, 172, 252, 413, 425, 761, 980, 1292
TspGWI ACGGA 4 cut(s) 129, 524, 1239, 1275
TspRI CASTG 1 cut(s) 1311
Vha464I CTTAAG 1 cut(s) 641
XapI RAATTY 3 cut(s) 20, 74, 904
XceI RCATGY 1 cut(s) 1062
XmiI GTMKAC 2 cut(s) 677, 761
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.