pycom07g04330

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012545
Physical Location & Seq
Reverse (-)
3737360 .. 3738733
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g04330.1

Sequence Viewer

Length: 1374 bp
ATGATGGTTTCTGGATTATCGACTAGGTTGTTGAACATATGTATAGCTTCGTTTTGTAAGTCCCGGCTACTGGAGAGAGCAGAAGCTGTTATAATTGATGGCATAAGATTGGGGGTGATTCCAGATGTGGTAACTTACAATACTTTGCTCAATGCCTATTCTCGGTTTGTTAGCTTGGATTCAGCTTATTCTGTTGTTCATAGAATGAAAGAGGCTGGGATTAGTCCAGATGTCATTACATACAATTCTTTGTTATCCGGGGCCACCAGGAATTGCCTATTATCACAATCTCTGGATCTGTTTGAGGAAATGTTACAAGCGGGCATACACCCTAATGTATGGAGTTACAATATTCTGATGCATTGTTTCTTTAAGTTGGGAAAACCTGATGAAGCCAACAGAGTTTTTCAGGATATTTTACTTCGCAATCTCACTCCCCACCCAGCTACGTTTAACATAATGATTAATGGCCTTTGTAAAAATGAATACATTGATAATGCCCTTATGTTATTTAGGAATTTGCAACGTCATGGATTTGTTCCCCGATTAGTGACGTACAATATTCTTATCCACGGGCTATGCAAGGCACGCAGATTGGGGCAAGCAAGAAAAATGCTAAAGGAACTGGGGGAATCAGGTTATGAGCCAAATGCCATAACCTACACTACAGTTATGAAATGCTGCTTTAAGTTTAAGCAGTATGACGAAGCGCTCGAGATTATGTCAGAGATGAAGAGTAAAGGGTATACTTTTGATGGCTTTGCAAACTGCACAGTTGTTGCTGCTTTAGTTAAGACTGGGAGGATAGAAGAGGCAAATGCTTGCATGGAACAGACAATGAGAAATGGCATTGAACTTGATTTAGCGGCTTATAACACTTTACTTAATATGTATTGTAGAGAAGGTAAGTTTGAAGCTGCCTATAAGTTGATGGATGAAATAGAGAATGGAGGTCTGCAGTGTGACAAGTATACCCACACAATTATAATTGATGGATTGTGTAAGGCTGGTAATATTATCGGGGCTGAACAACATTTACAATATATGAAAATGATTGGCTTCCGTGAAAATTTGGTTGCCTTCAACTGCATGATTGATGGCTTGTGTAAAGCTGGTCAAATCGATCGTGCGATGGACTTGTATAAATCAATGGAGACTAAAGATTCCTTTACCTACACCTCTTTGGTGCACAATCTTTGCAAGGCTGGAAGGTTCCGTTGTGCGTCCAAGCTTATGATGAAATGTCTAAGAGATGGCAAGAAAATACTCAAGGCTACCCAACGAGCTGTCCTTGATGGTCTTCGTAGTACAGGGCATACAGATGAAGCAAGGAAGCTTCGGTGGAAAATTCAAGTGGCTCGAATATTACGTTAA

Protein Analysis

458

Amino Acids

51.76

Weight (kDa)

8.93

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 12 - 54 5.4e-06 PPR repeat family
PPR_3 PF13812 36 - 86 1e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 38 - 70 2e-06 PPR repeat
PPR_2 PF13041 41 - 87 2.7e-14 PPR repeat family
PPR_2 PF13041 76 - 125 4.1e-13 PPR repeat family
PPR_long PF17177 94 - 203 3.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 100 - 156 2.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 108 - 137 3.1e-06 PPR repeat
PPR_2 PF13041 111 - 160 2e-13 PPR repeat family
TPR_24 PF23276 127 - 241 7.9e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 143 - 172 2.1e-07 PPR repeat
PPR_2 PF13041 149 - 195 4.3e-14 PPR repeat family
PPR PF01535 150 - 179 2.2e-06 PPR repeat
TPR_24 PF23276 170 - 272 3.9e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 170 - 228 3.9e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 178 - 208 3.8e-09 PPR repeat
PPR_2 PF13041 182 - 230 5.4e-14 PPR repeat family
PPR PF01535 184 - 213 4.9e-06 PPR repeat
PPR_long PF17177 202 - 329 8e-07 Pentacotripeptide-repeat region of PRORP
PPR PF01535 219 - 248 7.6e-07 PPR repeat
TPR_24 PF23276 281 - 384 1.3e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 282 - 314 3.7e-08 PPR repeat
PPR_2 PF13041 287 - 335 8.3e-17 PPR repeat family
PPR PF01535 290 - 318 5.7e-06 PPR repeat
PPR_1 PF12854 318 - 350 2.3e-06 PPR repeat
PPR_1 PF12854 357 - 385 1.4e-08 PPR repeat
PPR_2 PF13041 357 - 401 1.8e-11 PPR repeat family
PPR PF01535 359 - 387 7.4e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012545)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46680
fragaria_vesca FvH4_6g27690
malus_domestica MD07G1055100.v1.1 MD15G1427800.v1.1
prunus_persica Prupe.3G114500_v2.0.a1 Prupe.3G114500_v2.0.a1 Prupe.4G280300_v2.0.a1
pyrus_communis pycom07g04330
rosa_chinensis RchiOBHm_Chr2g0130841
rosa_laevigata RLG00000019152
rosa_multiflora Rmu_sc0003629.1_g000016
rosa_roxburghii Rroxscaffold_2G00113500 Rroxscaffold_4G00318090
rosa_rugosa Rorug02G0293500 Rorug02G0293600
rosa_samantha Rh2AG344900 Rh2BG353300 Rh2DG371200
rosa_wichuraiana Rw2G027470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 92, 873, 986
AccI GTMKAC 2 cut(s) 746, 971
AciI CCGC 2 cut(s) 320, 866
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 3 cut(s) 517, 1069, 1347
AfaI GTAC 2 cut(s) 557, 1309
AfeI AGCGCT 1 cut(s) 711
AfiI CCNNNNNNNGG 2 cut(s) 70, 162
AgsI TTSAA 5 cut(s) 34, 854, 914, 1084, 1352
AjnI CCWGG 1 cut(s) 266
Alw21I GWGCWC 1 cut(s) 1191
Alw26I GTCTC 1 cut(s) 1148
Alw44I GTGCAC 1 cut(s) 1187
AlwI GGATC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 713
Aor51HI AGCGCT 1 cut(s) 711
AoxI GGCC 2 cut(s) 261, 469
ApaLI GTGCAC 1 cut(s) 1187
ApeKI GCWGC 3 cut(s) 681, 782, 917
ApoI RAATTY 3 cut(s) 517, 1069, 1347
AseI ATTAAT 1 cut(s) 465
AspLEI GCGC 1 cut(s) 712
AspS9I GGNCC 1 cut(s) 261
AsuC2I CCSGG 2 cut(s) 64, 259
AsuHPI GGTGA 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 1191
BbsI GAAGAC 1 cut(s) 1292
Bbv12I GWGCWC 1 cut(s) 1191
BbvI GCAGC 3 cut(s) 668, 769, 904
BccI CCATC 8 cut(s) 92, 749, 925, 986, 1091, 1126, 1247, 1289
BciT130I CCWGG 1 cut(s) 268
BcnI CCSGG 2 cut(s) 64, 259
BcoDI GTCTC 1 cut(s) 1148
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 2 cut(s) 666, 956
BfoI RGCGCY 1 cut(s) 713
BisI GCNGC 4 cut(s) 682, 783, 867, 918
BlsI GCNGC 4 cut(s) 683, 784, 868, 919
Bme1390I CCNGG 3 cut(s) 64, 259, 268
BmeT110I CYCGRG 1 cut(s) 713
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 2 cut(s) 262, 1214
BmrFI CCNGG 3 cut(s) 64, 259, 268
BmrI ACTGGG 2 cut(s) 635, 807
BmsI GCATC 1 cut(s) 348
BmuI ACTGGG 2 cut(s) 635, 807
BpiI GAAGAC 1 cut(s) 1292
BpmI CTGGAG 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 1253
BpuMI CCSGG 2 cut(s) 64, 259
Bsa29I ATCGAT 1 cut(s) 1122
BsaJI CCNNGG 2 cut(s) 258, 571
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 162
Bse1I ACTGG 3 cut(s) 75, 630, 802
BseBI CCWGG 1 cut(s) 268
BseCI ATCGAT 1 cut(s) 1122
BseDI CCNNGG 2 cut(s) 258, 571
BseGI GGATG 1 cut(s) 940
BseLI CCNNNNNNNGG 2 cut(s) 70, 162
BseNI ACTGG 3 cut(s) 75, 630, 802
BseSI GKGCMC 1 cut(s) 1191
BseXI GCAGC 3 cut(s) 668, 769, 904
BseYI CCCAGC 2 cut(s) 215, 442
BsgI GTGCAG 1 cut(s) 754
Bsh1285I CGRYCG 1 cut(s) 1126
BshFI GGCC 2 cut(s) 263, 471
BshVI ATCGAT 1 cut(s) 1122
BsiEI CGRYCG 1 cut(s) 1126
BsiHKAI GWGCWC 1 cut(s) 1191
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 2 cut(s) 64, 258
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 2 cut(s) 70, 162
BsmAI GTCTC 1 cut(s) 1148
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 2 cut(s) 263, 471
BsoBI CYCGRG 1 cut(s) 713
Bsp1286I GDGCHC 1 cut(s) 1191
Bsp143I GATC 2 cut(s) 295, 1123
BspACI CCGC 2 cut(s) 320, 866
BspANI GGCC 2 cut(s) 263, 471
BspDI ATCGAT 1 cut(s) 1122
BspLI GGNNCC 2 cut(s) 262, 1214
BspMAI CTGCAG 1 cut(s) 960
BspPI GGATC 1 cut(s) 303
BsrI ACTGG 3 cut(s) 75, 630, 802
BssECI CCNNGG 2 cut(s) 258, 571
BssMI GATC 2 cut(s) 295, 1123
BssNAI GTATAC 2 cut(s) 747, 972
Bst1107I GTATAC 2 cut(s) 747, 972
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 2 cut(s) 670, 775
Bst6I CTCTTC 2 cut(s) 728, 804
BstC8I GCNNGC 4 cut(s) 322, 589, 603, 823
BstDEI CTNAG 1 cut(s) 1247
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 1 cut(s) 940
BstH2I RGCGCY 1 cut(s) 713
BstHHI GCGC 1 cut(s) 712
BstKTI GATC 2 cut(s) 298, 1126
BstMAI GTCTC 1 cut(s) 1148
BstMBI GATC 2 cut(s) 295, 1123
BstMCI CGRYCG 1 cut(s) 1126
BstMWI GCNNNNNNNGC 1 cut(s) 588
BstNI CCWGG 1 cut(s) 268
BstSCI CCNGG 3 cut(s) 62, 257, 266
BstSFI CTRYAG 2 cut(s) 666, 956
BstSLI GKGCMC 1 cut(s) 1191
BstV1I GCAGC 3 cut(s) 668, 769, 904
BstV2I GAAGAC 1 cut(s) 1292
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZ17I GTATAC 2 cut(s) 747, 972
Bsu15I ATCGAT 1 cut(s) 1122
BsuRI GGCC 2 cut(s) 263, 471
BsuTUI ATCGAT 1 cut(s) 1122
BtgI CCRYGG 1 cut(s) 571
BtgZI GCGATG 1 cut(s) 1145
BtsCI GGATG 1 cut(s) 940
BtsI GCAGTG 1 cut(s) 965
BtsIMutI CAGTG 1 cut(s) 965
Cac8I GCNNGC 4 cut(s) 322, 589, 603, 823
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 261
ClaI ATCGAT 1 cut(s) 1122
CseI GACGC 1 cut(s) 1212
Csp6I GTAC 2 cut(s) 556, 1308
CviAII CATG 3 cut(s) 530, 826, 1090
CviQI GTAC 2 cut(s) 556, 1308
DdeI CTNAG 1 cut(s) 1247
DpnI GATC 2 cut(s) 297, 1125
DpnII GATC 2 cut(s) 295, 1123
Eam1104I CTCTTC 2 cut(s) 728, 804
EarI CTCTTC 2 cut(s) 728, 804
Eco47III AGCGCT 1 cut(s) 711
Eco88I CYCGRG 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 266
EcoT22I ATGCAT 1 cut(s) 363
FaeI CATG 3 cut(s) 533, 829, 1093
FaqI GGGAC 1 cut(s) 46
FatI CATG 3 cut(s) 529, 825, 1089
FauI CCCGC 1 cut(s) 313
FauNDI CATATG 1 cut(s) 38
FblI GTMKAC 2 cut(s) 746, 971
Fnu4HI GCNGC 4 cut(s) 682, 783, 867, 918
FokI GGATG 1 cut(s) 947
Fsp4HI GCNGC 4 cut(s) 682, 783, 867, 918
FspBI CTAG 1 cut(s) 24
GlaI GCGC 1 cut(s) 711
GluI GCNGC 4 cut(s) 682, 783, 867, 918
GsaI CCCAGC 2 cut(s) 219, 446
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 713
HaeIII GGCC 2 cut(s) 263, 471
HapII CCGG 2 cut(s) 64, 258
HgaI GACGC 1 cut(s) 1212
HhaI GCGC 1 cut(s) 712
Hin1II CATG 3 cut(s) 533, 829, 1093
Hin6I GCGC 1 cut(s) 710
HinP1I GCGC 1 cut(s) 710
HindIII AAGCTT 2 cut(s) 1229, 1334
HinfI GANTC 4 cut(s) 118, 179, 632, 1163
HpaII CCGG 2 cut(s) 64, 258
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 3 cut(s) 747, 972, 1189
Hpy188I TCNGA 2 cut(s) 357, 727
Hpy188III TCNNGA 6 cut(s) 12, 122, 227, 293, 410, 715
Hpy8I GTNNAC 3 cut(s) 747, 972, 1189
HpyAV CCTTC 3 cut(s) 896, 1090, 1203
HpyCH4III ACNGT 2 cut(s) 670, 775
HpyCH4IV ACGT 4 cut(s) 449, 526, 554, 1369
HpyF10VI GCNNNNNNNGC 1 cut(s) 588
HpyF3I CTNAG 1 cut(s) 1247
HpySE526I ACGT 4 cut(s) 449, 526, 554, 1369
Hsp92II CATG 3 cut(s) 533, 829, 1093
HspAI GCGC 1 cut(s) 710
Kzo9I GATC 2 cut(s) 295, 1123
Lsp1109I GCAGC 3 cut(s) 668, 769, 904
LweI GCATC 1 cut(s) 348
MaeI CTAG 1 cut(s) 24
MaeII ACGT 4 cut(s) 449, 526, 554, 1369
MaeIII GTNAC 5 cut(s) 130, 312, 344, 550, 962
MalI GATC 2 cut(s) 297, 1125
MboI GATC 2 cut(s) 295, 1123
MboII GAAGA 3 cut(s) 745, 821, 1292
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 1191
MluCI AATT 8 cut(s) 93, 244, 271, 517, 981, 987, 1069, 1347
MnlI CCTC 6 cut(s) 205, 298, 795, 805, 944, 1189
Mph1103I ATGCAT 1 cut(s) 363
MseI TTAA 8 cut(s) 372, 453, 465, 687, 693, 792, 885, 1372
MslI CAYNNNNRTG 2 cut(s) 333, 1320
MspI CCGG 2 cut(s) 64, 258
MspR9I CCNGG 3 cut(s) 64, 259, 268
MvaI CCWGG 1 cut(s) 268
MwoI GCNNNNNNNGC 1 cut(s) 588
NciI CCSGG 2 cut(s) 64, 259
NdeI CATATG 1 cut(s) 38
NdeII GATC 2 cut(s) 295, 1123
NlaIII CATG 3 cut(s) 533, 829, 1093
NlaIV GGNNCC 2 cut(s) 262, 1214
NmuCI GTSAC 2 cut(s) 550, 962
NsiI ATGCAT 1 cut(s) 363
PaeR7I CTCGAG 1 cut(s) 713
PcsI WCGNNNNNNNCGW 2 cut(s) 711, 1366
PfeI GAWTC 4 cut(s) 118, 179, 632, 1163
PkrI GCNGC 4 cut(s) 683, 784, 868, 919
Ple19I CGATCG 1 cut(s) 1126
PshBI ATTAAT 1 cut(s) 465
PsiI TTATAA 3 cut(s) 92, 873, 986
Psp6I CCWGG 1 cut(s) 266
PspFI CCCAGC 2 cut(s) 215, 442
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 2 cut(s) 262, 1214
PspPI GGNCC 1 cut(s) 261
PsrI GAACNNNNNNTAC 2 cut(s) 1020, 1052
PstI CTGCAG 1 cut(s) 960
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 295
PvuI CGATCG 1 cut(s) 1126
RsaI GTAC 2 cut(s) 557, 1309
RsaNI GTAC 2 cut(s) 556, 1308
RseI CAYNNNNRTG 2 cut(s) 333, 1320
SaqAI TTAA 8 cut(s) 372, 453, 465, 687, 693, 792, 885, 1372
SatI GCNGC 4 cut(s) 682, 783, 867, 918
Sau3AI GATC 2 cut(s) 295, 1123
Sau96I GGNCC 1 cut(s) 261
ScrFI CCNGG 3 cut(s) 64, 259, 268
SduI GDGCHC 1 cut(s) 1191
SfaNI GCATC 1 cut(s) 348
SfcI CTRYAG 2 cut(s) 666, 956
Sfr274I CTCGAG 1 cut(s) 713
SlaI CTCGAG 1 cut(s) 713
SmiMI CAYNNNNRTG 2 cut(s) 333, 1320
SmlI CTYRAG 2 cut(s) 713, 1268
SmoI CTYRAG 2 cut(s) 713, 1268
Sse9I AATT 8 cut(s) 93, 244, 271, 517, 981, 987, 1069, 1347
SsiI CCGC 2 cut(s) 320, 866
SspI AATATT 4 cut(s) 352, 562, 1015, 1365
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 3 cut(s) 62, 257, 266
TaaI ACNGT 2 cut(s) 670, 775
TaiI ACGT 4 cut(s) 452, 529, 557, 1372
TaqI TCGA 4 cut(s) 20, 714, 1122, 1360
TasI AATT 8 cut(s) 93, 244, 271, 517, 981, 987, 1069, 1347
TatI WGTACW 1 cut(s) 1307
TauI GCSGC 1 cut(s) 869
TfiI GAWTC 4 cut(s) 118, 179, 632, 1163
Tru1I TTAA 8 cut(s) 372, 453, 465, 687, 693, 792, 885, 1372
Tru9I TTAA 8 cut(s) 372, 453, 465, 687, 693, 792, 885, 1372
TscAI CASTG 1 cut(s) 965
TseFI GTSAC 2 cut(s) 550, 962
TseI GCWGC 3 cut(s) 681, 782, 917
Tsp45I GTSAC 2 cut(s) 550, 962
TspGWI ACGGA 2 cut(s) 1052, 1205
TspRI CASTG 1 cut(s) 965
VneI GTGCAC 1 cut(s) 1187
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 3 cut(s) 517, 1069, 1347
XhoI CTCGAG 1 cut(s) 713
XmiI GTMKAC 2 cut(s) 746, 971
XspI CTAG 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.