Rh2AG344900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012545
Physical Location & Seq
Forward (+)
50713701 .. 50718915
5215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG344900.1

Sequence Viewer

Length: 1374 bp
ATGATGGTCTGTAGATTATCGACTTTGCTGTTGAACGTATGCATATCTTCATTTTGTAAAGTCCGGCTATTGGGGAGAGCGGAGGCTGTTATAATTGATGGTATAAGATTAGGGGTGATTCCAGATGTAGTCACTTACAATACTTTGGTTCATGCATATTGTCAGTTTGTTAGCGTAGATGCGGCTTATTCCGTTGTTCATAGAATGAAAGAAGCTGGGATAAGTCCGGATGTCGTTACTTACAATTCTTTGATATCAGGGGCCACCAGGAATCGCTTATTATCTCGAGTCCTGGATCTGTTTGAGGAAATGTTACAGGAAGGTATACATCCTGACGTGTGGAGTTACAATATTCTGATGCACTGTTTCTTTAAGTTAGGGAAACCTGATGAAGCCAACAGAGTCTTCCAGGATATTTTACTTAGCGACCTCACTCCTCATGCAGCTACATTTAATATTATGATTAATGGCCTTTGTAAAAATGGGTACACGGACAATGCTCTTATGTTATTTAGGAATTTACAACGTCATGGATTTGTTCCCCCGTTAGTGACATACAATATTCTTATCAGTGGGCTTTGCAAGGCACGTAGAGTGGGGCAAGCGAGGAGAATTCTAAAGGACCTTGGAGAATCAAGTCATAAGCCAAATGCCGTAACCTACACTACAGTTATGAGTTGCTGCTTTAGGTCTAAGCAATACGACCAAGGGCTTGAGATTATGTCAGAGATGAAGAGTAAAGGGTATACATTTGACGGCTTTGCGTACTGCACAGTCATTGCTGCTTTACTTAAGACTGGAAGGATGGAAGGAGCAAATGCTTTCATGGAACAGATGATGAATAGTGGCATTGAAATGGATTTGGTGTCTTATAACACCTTACTTAATAAGCTTTGTAAAGAAGGCAAGGTTGAAGCTGCCTATAAGTTGTTGGATGAAATAGAGAATGGGGGCCTTTTGTGTGATAAGTATACCCATACAATCATGATTGATGGATTGTGTAAGGCGGGCAATATTGCTGGTGCTCAACAACATTTACATTACATGAATATGATGGGATTCCAGGAAAACTTGGTTGCCTTTAACTGTCTGATTGATGGCTTGGGTAAAGCTGGTGAAATTGATCGTGCAATGGAATTGTACAATTCAATGGAAACTAGGGATTCTTTTACTTATGACTCCTTGGTGCTCAATCTTTGCAAGGCTGGAAGGGTCCTTTGTGCATCTAAGCTTATGATGAAATGTTTAAGAGAGGGAATGATATTACTCTACTCTACCAGGCGAGCACTCCTTGATGGGCTTCGTTCTTCAGGGTTTACAAATGAAGCAAGAAAGCTTCAATCAAAAATTCGGTTAGCTCGGCTGTTACGTTAA

Protein Analysis

457

Amino Acids

51.23

Weight (kDa)

8.65

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 36 - 86 8.8e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 38 - 70 5.7e-09 PPR repeat
PPR_2 PF13041 41 - 87 1.3e-15 PPR repeat family
TPR_24 PF23276 44 - 138 3.4e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 73 - 104 7.6e-07 PPR repeat
PPR_2 PF13041 76 - 125 1e-14 PPR repeat family
PPR PF01535 79 - 109 4.1e-07 PPR repeat
PPR_3 PF13812 100 - 156 8.4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 108 - 137 2e-06 PPR repeat
PPR_2 PF13041 111 - 160 7.6e-13 PPR repeat family
PPR_1 PF12854 144 - 172 1.1e-08 PPR repeat
PPR_2 PF13041 146 - 195 8e-15 PPR repeat family
PPR PF01535 150 - 179 2.1e-07 PPR repeat
PPR_3 PF13812 170 - 228 4.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 178 - 207 5.8e-08 PPR repeat
PPR_2 PF13041 183 - 229 1.6e-12 PPR repeat family
PPR_long PF17177 201 - 315 3.7e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 216 - 264 1e-09 PPR repeat family
PPR PF01535 219 - 248 8.4e-06 PPR repeat
PPR_2 PF13041 254 - 299 1.9e-09 PPR repeat family
TPR_24 PF23276 278 - 384 1.4e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 283 - 314 1.8e-10 PPR repeat
PPR_2 PF13041 287 - 335 4.6e-17 PPR repeat family
PPR PF01535 289 - 318 5.1e-06 PPR repeat
PPR_1 PF12854 318 - 349 6.3e-07 PPR repeat
PPR_1 PF12854 354 - 385 1.4e-07 PPR repeat
PPR_2 PF13041 357 - 401 3.7e-08 PPR repeat family
PPR PF01535 359 - 387 2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012545)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46680
fragaria_vesca FvH4_6g27690
malus_domestica MD07G1055100.v1.1 MD15G1427800.v1.1
prunus_persica Prupe.3G114500_v2.0.a1 Prupe.3G114500_v2.0.a1 Prupe.4G280300_v2.0.a1
pyrus_communis pycom07g04330
rosa_chinensis RchiOBHm_Chr2g0130841
rosa_laevigata RLG00000019152
rosa_multiflora Rmu_sc0003629.1_g000016
rosa_roxburghii Rroxscaffold_2G00113500 Rroxscaffold_4G00318090
rosa_rugosa Rorug02G0293500 Rorug02G0293600
rosa_samantha Rh2AG344900 Rh2BG353300 Rh2DG371200
rosa_wichuraiana Rw2G027470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 92, 873
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 3 cut(s) 325, 746, 971
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 3 cut(s) 80, 182, 1007
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 3 cut(s) 517, 612, 1347
AcuI CTGAAG 1 cut(s) 1293
AfaI GTAC 3 cut(s) 488, 767, 1142
AfiI CCNNNNNNNGG 1 cut(s) 70
AflII CTTAAG 1 cut(s) 791
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 5 cut(s) 34, 854, 914, 1149, 1340
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 5 cut(s) 266, 291, 408, 1062, 1277
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AluBI AGCT 8 cut(s) 215, 446, 892, 917, 1112, 1231, 1336, 1358
AluI AGCT 8 cut(s) 215, 446, 892, 917, 1112, 1231, 1336, 1358
Alw21I GWGCWC 3 cut(s) 1027, 1191, 1288
AlwI GGATC 1 cut(s) 303
Ama87I CYCGRG 1 cut(s) 285
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 3 cut(s) 261, 469, 952
ApeKI GCWGC 4 cut(s) 443, 681, 782, 917
ApoI RAATTY 3 cut(s) 517, 612, 1347
AseI ATTAAT 1 cut(s) 465
AspS9I GGNCC 4 cut(s) 261, 622, 952, 1213
AsuHPI GGTGA 2 cut(s) 127, 1127
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 2 cut(s) 622, 1213
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 3 cut(s) 1027, 1191, 1288
BbvI GCAGC 4 cut(s) 455, 668, 769, 904
BccI CCATC 6 cut(s) 92, 799, 986, 1048, 1091, 1289
BceAI ACGGC 2 cut(s) 638, 772
BciT130I CCWGG 5 cut(s) 268, 293, 410, 1064, 1279
BfaI CTAG 1 cut(s) 1158
BfmI CTRYAG 2 cut(s) 10, 666
BfrI CTTAAG 1 cut(s) 791
BisI GCNGC 5 cut(s) 183, 444, 682, 783, 918
BlsI GCNGC 5 cut(s) 184, 445, 683, 784, 919
Bme1390I CCNGG 5 cut(s) 268, 293, 410, 1064, 1279
Bme18I GGWCC 2 cut(s) 622, 1213
BmeT110I CYCGRG 1 cut(s) 285
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 4 cut(s) 261, 622, 952, 1213
BmiI GGNNCC 3 cut(s) 262, 953, 1214
BmrFI CCNGG 5 cut(s) 268, 293, 410, 1064, 1279
BmsI GCATC 3 cut(s) 169, 348, 1232
BpiI GAAGAC 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 734
BsaAI YACGTR 1 cut(s) 590
BsaJI CCNNGG 3 cut(s) 625, 706, 1182
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 802
Bse3DI GCAATG 2 cut(s) 777, 1137
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 5 cut(s) 268, 293, 410, 1064, 1279
BseDI CCNNGG 3 cut(s) 625, 706, 1182
BseGI GGATG 4 cut(s) 235, 328, 810, 940
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMI GCAATG 2 cut(s) 777, 1137
BseNI ACTGG 1 cut(s) 802
BseRI GAGGAG 2 cut(s) 426, 622
BseXI GCAGC 4 cut(s) 455, 668, 769, 904
BseYI CCCAGC 1 cut(s) 215
BsgI GTGCAG 1 cut(s) 754
BshFI GGCC 3 cut(s) 263, 471, 954
BsiHKAI GWGCWC 3 cut(s) 1027, 1191, 1288
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 2 cut(s) 64, 227
BslI CCNNNNNNNGG 1 cut(s) 70
BsnI GGCC 3 cut(s) 263, 471, 954
BsoBI CYCGRG 1 cut(s) 285
Bsp1286I GDGCHC 3 cut(s) 1027, 1191, 1288
Bsp13I TCCGGA 1 cut(s) 226
Bsp1407I TGTACA 1 cut(s) 1140
Bsp143I GATC 2 cut(s) 295, 1123
BspACI CCGC 3 cut(s) 80, 182, 1007
BspANI GGCC 3 cut(s) 263, 471, 954
BspEI TCCGGA 1 cut(s) 226
BspHI TCATGA 1 cut(s) 984
BspLI GGNNCC 3 cut(s) 262, 953, 1214
BspPI GGATC 1 cut(s) 303
BspTI CTTAAG 1 cut(s) 791
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 2 cut(s) 777, 1137
BsrGI TGTACA 1 cut(s) 1140
BsrI ACTGG 1 cut(s) 802
BssECI CCNNGG 3 cut(s) 625, 706, 1182
BssMI GATC 2 cut(s) 295, 1123
BssNAI GTATAC 3 cut(s) 326, 747, 972
BssT1I CCWWGG 3 cut(s) 625, 706, 1182
Bst1107I GTATAC 3 cut(s) 326, 747, 972
Bst2UI CCWGG 5 cut(s) 268, 293, 410, 1064, 1279
Bst4CI ACNGT 4 cut(s) 365, 670, 775, 1088
Bst6I CTCTTC 1 cut(s) 728
BstAFI CTTAAG 1 cut(s) 791
BstAUI TGTACA 1 cut(s) 1140
BstBAI YACGTR 1 cut(s) 590
BstC8I GCNNGC 3 cut(s) 603, 1009, 1284
BstDEI CTNAG 3 cut(s) 422, 693, 1227
BstF5I GGATG 4 cut(s) 235, 328, 810, 940
BstKTI GATC 2 cut(s) 298, 1126
BstMBI GATC 2 cut(s) 295, 1123
BstNI CCWGG 5 cut(s) 268, 293, 410, 1064, 1279
BstSCI CCNGG 5 cut(s) 266, 291, 408, 1062, 1277
BstSFI CTRYAG 2 cut(s) 10, 666
BstV1I GCAGC 4 cut(s) 455, 668, 769, 904
BstV2I GAAGAC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZ17I GTATAC 3 cut(s) 326, 747, 972
BsuRI GGCC 3 cut(s) 263, 471, 954
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 4 cut(s) 235, 328, 810, 940
BtsIMutI CAGTG 2 cut(s) 361, 577
Cac8I GCNNGC 3 cut(s) 603, 1009, 1284
CciI TCATGA 1 cut(s) 984
Cfr13I GGNCC 4 cut(s) 261, 622, 952, 1213
Csp6I GTAC 3 cut(s) 487, 766, 1141
CviAII CATG 6 cut(s) 152, 440, 530, 826, 985, 1045
CviQI GTAC 3 cut(s) 487, 766, 1141
DdeI CTNAG 3 cut(s) 422, 693, 1227
DpnI GATC 2 cut(s) 297, 1125
DpnII GATC 2 cut(s) 295, 1123
Eam1104I CTCTTC 1 cut(s) 728
EarI CTCTTC 1 cut(s) 728
Eco130I CCWWGG 3 cut(s) 625, 706, 1182
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 622, 1213
Eco57I CTGAAG 1 cut(s) 1293
Eco88I CYCGRG 1 cut(s) 285
EcoO109I RGGNCCY 3 cut(s) 622, 952, 1213
EcoRI GAATTC 1 cut(s) 612
EcoRII CCWGG 5 cut(s) 266, 291, 408, 1062, 1277
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 3 cut(s) 625, 706, 1182
EcoT22I ATGCAT 2 cut(s) 44, 157
ErhI CCWWGG 3 cut(s) 625, 706, 1182
FaeI CATG 6 cut(s) 155, 443, 533, 829, 988, 1048
FatI CATG 6 cut(s) 151, 439, 529, 825, 984, 1044
FauI CCCGC 1 cut(s) 1000
FblI GTMKAC 3 cut(s) 325, 746, 971
Fnu4HI GCNGC 5 cut(s) 183, 444, 682, 783, 918
FokI GGATG 4 cut(s) 242, 315, 817, 947
Fsp4HI GCNGC 5 cut(s) 183, 444, 682, 783, 918
FspBI CTAG 1 cut(s) 1158
GluI GCNGC 5 cut(s) 183, 444, 682, 783, 918
GsaI CCCAGC 1 cut(s) 219
HaeIII GGCC 3 cut(s) 263, 471, 954
HapII CCGG 2 cut(s) 64, 227
Hin1II CATG 6 cut(s) 155, 443, 533, 829, 988, 1048
HindIII AAGCTT 3 cut(s) 890, 1229, 1334
HinfI GANTC 8 cut(s) 118, 271, 288, 402, 632, 1059, 1163, 1178
HpaII CCGG 2 cut(s) 64, 227
HphI GGTGA 2 cut(s) 127, 1127
Hpy166II GTNNAC 5 cut(s) 326, 489, 747, 972, 1317
Hpy188I TCNGA 3 cut(s) 357, 727, 1092
Hpy188III TCNNGA 5 cut(s) 122, 227, 285, 332, 985
Hpy8I GTNNAC 5 cut(s) 326, 489, 747, 972, 1317
HpyAV CCTTC 5 cut(s) 314, 795, 803, 896, 1203
HpyCH4III ACNGT 4 cut(s) 365, 670, 775, 1088
HpyCH4IV ACGT 5 cut(s) 36, 336, 526, 589, 1369
HpyCH4V TGCA 9 cut(s) 42, 155, 361, 443, 582, 771, 1130, 1200, 1223
HpyF3I CTNAG 3 cut(s) 422, 693, 1227
HpySE526I ACGT 5 cut(s) 36, 336, 526, 589, 1369
Hsp92II CATG 6 cut(s) 155, 443, 533, 829, 988, 1048
Kpn2I TCCGGA 1 cut(s) 226
Kzo9I GATC 2 cut(s) 295, 1123
LmnI GCTCC 1 cut(s) 812
Lsp1109I GCAGC 4 cut(s) 455, 668, 769, 904
LweI GCATC 3 cut(s) 169, 348, 1232
MaeI CTAG 1 cut(s) 1158
MaeII ACGT 5 cut(s) 36, 336, 526, 589, 1369
MaeIII GTNAC 7 cut(s) 130, 235, 312, 344, 550, 655, 1365
MalI GATC 2 cut(s) 297, 1125
MbiI CCGCTC 1 cut(s) 80
MboI GATC 2 cut(s) 295, 1123
MboII GAAGA 4 cut(s) 39, 397, 745, 1299
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 3 cut(s) 1027, 1191, 1288
MluCI AATT 8 cut(s) 93, 244, 517, 612, 1119, 1136, 1144, 1347
MlyI GAGTC 3 cut(s) 297, 411, 1172
MmeI TCCRAC 1 cut(s) 912
MnlI CCTC 6 cut(s) 76, 298, 440, 447, 600, 1246
Mph1103I ATGCAT 2 cut(s) 44, 157
MroI TCCGGA 1 cut(s) 226
MseI TTAA 8 cut(s) 372, 453, 465, 792, 885, 1083, 1247, 1372
MslI CAYNNNNRTG 2 cut(s) 854, 1049
MspCI CTTAAG 1 cut(s) 791
MspI CCGG 2 cut(s) 64, 227
MspR9I CCNGG 5 cut(s) 268, 293, 410, 1064, 1279
MvaI CCWGG 5 cut(s) 268, 293, 410, 1064, 1279
NdeII GATC 2 cut(s) 295, 1123
NlaIII CATG 6 cut(s) 155, 443, 533, 829, 988, 1048
NlaIV GGNNCC 3 cut(s) 262, 953, 1214
NmeAIII GCCGAG 1 cut(s) 1339
NmuCI GTSAC 2 cut(s) 130, 550
NsiI ATGCAT 2 cut(s) 44, 157
PaeR7I CTCGAG 1 cut(s) 285
PagI TCATGA 1 cut(s) 984
PcsI WCGNNNNNNNCGW 1 cut(s) 1366
PfeI GAWTC 5 cut(s) 118, 271, 632, 1059, 1163
PfoI TCCNGGA 3 cut(s) 291, 408, 1062
PkrI GCNGC 5 cut(s) 184, 445, 683, 784, 919
PleI GAGTC 3 cut(s) 296, 410, 1172
PpsI GAGTC 3 cut(s) 296, 410, 1172
Ppu21I YACGTR 1 cut(s) 590
PpuMI RGGWCCY 2 cut(s) 622, 1213
PshBI ATTAAT 1 cut(s) 465
PsiI TTATAA 2 cut(s) 92, 873
Psp5II RGGWCCY 2 cut(s) 622, 1213
Psp6I CCWGG 5 cut(s) 266, 291, 408, 1062, 1277
PspFI CCCAGC 1 cut(s) 215
PspGI CCWGG 5 cut(s) 266, 291, 408, 1062, 1277
PspN4I GGNNCC 3 cut(s) 262, 953, 1214
PspPI GGNCC 4 cut(s) 261, 622, 952, 1213
PspPPI RGGWCCY 2 cut(s) 622, 1213
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 3 cut(s) 488, 767, 1142
RsaNI GTAC 3 cut(s) 487, 766, 1141
RseI CAYNNNNRTG 2 cut(s) 854, 1049
SaqAI TTAA 8 cut(s) 372, 453, 465, 792, 885, 1083, 1247, 1372
SatI GCNGC 5 cut(s) 183, 444, 682, 783, 918
Sau3AI GATC 2 cut(s) 295, 1123
Sau96I GGNCC 4 cut(s) 261, 622, 952, 1213
SchI GAGTC 3 cut(s) 297, 411, 1172
ScrFI CCNGG 5 cut(s) 268, 293, 410, 1064, 1279
SduI GDGCHC 3 cut(s) 1027, 1191, 1288
SfaNI GCATC 3 cut(s) 169, 348, 1232
SfcI CTRYAG 2 cut(s) 10, 666
Sfr274I CTCGAG 1 cut(s) 285
SinI GGWCC 2 cut(s) 622, 1213
SlaI CTCGAG 1 cut(s) 285
SmiMI CAYNNNNRTG 2 cut(s) 854, 1049
SmlI CTYRAG 3 cut(s) 285, 713, 791
SmoI CTYRAG 3 cut(s) 285, 713, 791
Sse9I AATT 8 cut(s) 93, 244, 517, 612, 1119, 1136, 1144, 1347
SsiI CCGC 3 cut(s) 80, 182, 1007
SspI AATATT 4 cut(s) 352, 457, 562, 1015
SspMI CTAG 1 cut(s) 1158
StyD4I CCNGG 5 cut(s) 266, 291, 408, 1062, 1277
StyI CCWWGG 3 cut(s) 625, 706, 1182
TaaI ACNGT 4 cut(s) 365, 670, 775, 1088
TaiI ACGT 5 cut(s) 39, 339, 529, 592, 1372
TaqI TCGA 2 cut(s) 20, 286
TasI AATT 8 cut(s) 93, 244, 517, 612, 1119, 1136, 1144, 1347
TatI WGTACW 1 cut(s) 1140
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 5 cut(s) 118, 271, 632, 1059, 1163
Tru1I TTAA 8 cut(s) 372, 453, 465, 792, 885, 1083, 1247, 1372
Tru9I TTAA 8 cut(s) 372, 453, 465, 792, 885, 1083, 1247, 1372
TscAI CASTG 2 cut(s) 368, 577
TseFI GTSAC 2 cut(s) 130, 550
TseI GCWGC 4 cut(s) 443, 681, 782, 917
Tsp45I GTSAC 2 cut(s) 130, 550
TspGWI ACGGA 2 cut(s) 181, 506
TspRI CASTG 2 cut(s) 368, 577
Vha464I CTTAAG 1 cut(s) 791
VpaK11BI GGWCC 2 cut(s) 622, 1213
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 3 cut(s) 517, 612, 1347
XhoI CTCGAG 1 cut(s) 285
XmiI GTMKAC 3 cut(s) 325, 746, 971
XspI CTAG 1 cut(s) 1158
Zsp2I ATGCAT 2 cut(s) 44, 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.