AT5G47010

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009459
Physical Location & Seq
Forward (+)
19071824 .. 19079453
7630 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47010.1

Sequence Viewer

Length: 3765 bp
ATGGATTCTCAACAGAGCGATCTCTTTGACACCGCATCGCAGCCGGACACGGTGGCCGACGAGTATACCTTTTTGGAATTCAATACTCAAGGCGATTCAGAGTTCGATTACCAGGATTTTGGATCGCCGACGGCATGGCCGACACCGTCTGATTCTATTTCCATCGCTGACGTTGCCGATCGTGGTGAAGGAGGAGCTGCCGCGGATCATCATTCTGAGGCTTCGTCACCTTCGTCCCTCTCTGCCGGTGCCGGGAATGGTGCGAAAGTGGGACGCGGTGGAGTTGGTGGAAGTGGTGGTGTTAGTAGTAGTAGTCAGGTTGATGCATTGGCCGCTGGTGTGGGAAATTTGAACTTTGAAGAGACTGGTGATGATGATGGTTTTGATTATGGTAAAAATGATTTCACGGAGCACGCTTGTAAGTATTGTGGTATCTCGAACCCAGCTTGCGTCGTTAGGTGTAATGTGGCTTCTTGTAGAAAGTGGTTCTGCAATTCAAGGGGAAACACCTCAGGCTCTCATATTGTGAATCACCTGGTGCGAGCAAAACACAAGGAAGTTTGTCTCCATAGAGACAGTCCACTAGGGGAAACAATTCTCGAATGCTACAACTGTGGGTGTCGGAATGTCTTTCTTCTCGGTTTCATCTCAGCAAAGACAGATAGTGTTGTCGTCCTTCTCTGTAGAGATCCTTGTCTAAATGTAAATGCCCTGAAGGATATGAACTGGGACCTAAGTCAGTGGTGTCCCTTGATTGACGACAGATGTTTCCTCCCCTGGCTTGTTAAGGTCCCATCAGAGCAAGAACAGCTAAGGGCACGCCAAATTAGCGCACAGCAAATAAACAAGATAGAGGAATTATGGAAGACAAATCCGGATGCTACTCTTGAAGACCTTGAAAAACCTGGTGTAGATGATGAACCTCAGCCTGTTCAACCTAAGTATGAAGATGCGTACCAGTATCAAAATGTGTTTGCACCTCTAATCAAACTCGAAGCAGACTATGACAAGATGATGAAAGAGTCTCAAAGCAAGGAAAACCTCACTGTTCGGTGGGATATTGGTCTTAACAAGAAGCGTGTTGCATACTTCGTCTTCCCAAAGGAGGAAAATGAATTGCGATTGGTACCGGGTGATGAACTACGTCTGCGGTACTCGGGAGATGCTGTTCATCCATCCTGGCAGTCAGTTGGACATGTGATCAAGCTAACGGCGCAAGAGGAGGTTGCTCTTGAACTCCGAGCTAATCAGGGAGTTCCGATTGACGTAAATCATGGATTCAGTGTTGATTTTGTTTGGAAAAGTACAAGCTTTGATCGAATGCAGGGAGCAATGAAGAATTTTGCTGTAGATGAAACAAGTGTGAGTGGTTATATCTACCATCAGTTATTGGGACATGAAGTTGAGGCCCAGATGGTTCGTAACACACTACCTCGACGTTTTGGGGTACCTGGTCTTCCAGAACTGAATGCATCTCAGGTTAATGCGGTGAAAAGTGTCCTTCAGAAGCCCATCAGCTTGATCCAAGGTCCACCTGGCACAGGCAAAACTGTGACTTCTGCAGCAATTGTGTATCACATGGCAAAACAGGGCCAGGGACAGGTTCTCGTTTGTGCCCCAAGTAATGTTGCTGTGGACCAACTAGCTGAAAAAATTAGTGCTACTGGGTTAAAGGTTGTTCGCCTTTGCGCAAAATCAAGGGAAGCTGTAAGTTCTCCTGTAGAGTATTTGACCCTTCACTACCAGGTAAGACATCTTGACACATCTGAAAAGAGCGAACTGCATAAGTTGCAGCAGTTGAAAGATGAACAAGGTGAGCTGTCAAGCAGTGATGAAAAGAAATACAAAAATTTGAAAAGAGCGACAGAACGCGAGATAACCCAGAGTGCTGATGTCATATGCTGCACATGTGTTGGTGCTGCTGATCTTCGGTTGTCAAACTTTAGATTTAGACAGGTACTTATTGATGAGTCTACTCAAGCAACAGAACCTGAGTGCCTTATCCCTTTGGTCCTTGGAGTAAAACAGGTTGTTCTTGTTGGTGATCATTGTCAGCTTGGCCCCGTCATTATGTGCAAAAAAGCAGCTCGTGCTGGCTTGGCACAGTCTCTCTTTGAACGACTTGTGACTCTTGGTATTAAACCAATCAGATTGCAGGTTCAATATCGTATGCATCCAGCTTTGTCCGAGTTTCCATCCAACAGCTTCTATGAAGGAACTCTACAGAATGGAGTCACAATTATTGAAAGGCAAACAACAGGGATTGATTTCCCGTGGCCTGTTCCTAACCGGCCCATGTTTTTCTATGTTCAGCTGGGACAAGAGGAGATCAGTGCTAGTGGAACATCATATCTTAATAGAACTGAGGCTGCTAATGTGGAGAAACTTGTGACTGCTTTCTTAAAGAGTGGAGTTGTCCCCAGTCAGATTGGAGTTATAACTCCATATGAGGGACAGAGGGCATACATTGTTAATTACATGGCAAGAAATGGTTCTCTCCGACAGCAACTGTACAAGGAAATTGAGGTTGCCAGTGTTGATTCTTTTCAAGGAAGGGAAAAAGATTACATCATATTGTCCTGTGTGAGAAGTAATGAGCATCAGGGCATTGGATTCCTTAATGATCCACGGAGGCTTAATGTTGCACTTACGCGAGCTCGTTATGGAATTGTTATTCTTGGAAACCCAAAAGTTCTTAGTAAACAGCCTCTGTGGAATGGATTGTTGACACATTACAAGGAACATGAGTGCTTGGTCGAGGGACCTCTTAATAATCTAAAGCAGAGCATGGTACAGTTTCAGAAACCAAGAAAGATTTACAATGATCGGAGACTATTTTATGGTGGTGGAGCTGGCATGATTGGAAATGATAATTTTGGTTCGGGTAACCCTAATGCTGACAGAAGAGGCAGTCGAGGAAGGGCTGGTGGTTCCTATCTTCCTTCTGGCCCACCTAATGGTGCTAGACCTGGACTACACCCGGCAGGGTACCCGATACCTAGGGTTCCACTTTCACCCTTTCCTGGTGGTCCTCCTTCTCAGCCATACGCTATTCCAACTCGTGGACCTGTTGGTGCTGTTCCTCATGCTCCTCAACCTGGAAACCATGGTTTTGGAGCTGGTCGTGGAACTTCAGTTGGGGGGCACCTTCCGCACCAACAAGCCACCCAACATAATGTTGGAACTATTGGCCCTAGTTTAAACTTTCCCCTTGATAGTCCGAATAGCCAGCCTTCACCTGGTGGTCCGCTATCCCAACCTGGATATGGTAGCCAAGCGTTCAGGGATGGATTTTCTATGGGTGGAATATCTCAGGATTTCTTGGCTGATGATATCAAGAGCCAGGGATCTCATGATCCTTACAACATGGCCGACTTTGCTACACAGGCCTCACCGGGTGGATTTGCTGTTGATTATGCCACACAAGGAGCACATGGGGCGTTTCCTGGAAACTTTATGAATCAGAATTCTCAAGGCGGTTATTCTCGTTTCAGCGGAATTAATGATTTCATGTCTCAGGAGTATATGGCACATGGAGGTCAAGGTCTTTTCACCCAAGCTGGCTTTATCGACTCATCCCAGGATGATGGACAGCAGAATCCTTATGGTGTGAACAATCCAAATCTTCAGTCTCAGGGTCTCCCAAATTCGCTCTACTCGCAGCCATTCGCACACTACAACACACAGCCACTAAACCTTTCAGGCCCACAGCAATCTCAACCTAACCAGAGCTCACAAAACCCAAAACATCCTTACAATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

1254

Amino Acids

136.87

Weight (kDa)

6.04

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 138 - 291 2.2e-73 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 343 - 432 7.6e-27 RNA helicase UPF1, 1B domain
AAA_11 PF13086 488 - 607 3.8e-24 AAA domain
AAA_30 PF13604 488 - 689 3.3e-14 AAA domain
ResIII PF04851 488 - 558 1.9e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 493 - 689 1.5e-11 AAA domain
AAA_11 PF13086 590 - 691 1.3e-28 AAA domain
AAA_12 PF13087 700 - 896 7.8e-62 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2438
Acc16I TGCGCA 1 cut(s) 1690
Acc36I ACCTGC 1 cut(s) 2146
Acc65I GGTACC 3 cut(s) 1126, 1447, 2988
AccB1I GGYRCC 5 cut(s) 248, 1126, 1447, 2988, 3144
AccB7I CCANNNNNTGG 2 cut(s) 2957, 3062
AccI GTMKAC 2 cut(s) 65, 1973
AccII CGCG 4 cut(s) 203, 276, 1872, 2653
AccIII TCCGGA 1 cut(s) 874
AclWI GGATC 7 cut(s) 130, 213, 683, 1516, 2618, 3350, 3355
AcoI YGGCCR 4 cut(s) 54, 137, 330, 3369
AcsI RAATTY 6 cut(s) 77, 346, 1339, 1849, 3466, 3646
AcuI CTGAAG 4 cut(s) 734, 1487, 3117, 3611
AdeI CACNNNGTG 3 cut(s) 538, 3242, 3398
AfaI GTAC 9 cut(s) 956, 1128, 1154, 1306, 1449, 1959, 2513, 2793, 2990
AflIII ACRYGT 2 cut(s) 1195, 1907
AloI GAACNNNNNNTCC 2 cut(s) 1152, 1184
Alw21I GWGCWC 4 cut(s) 414, 2659, 3433, 3734
Alw26I GTCTC 9 cut(s) 356, 567, 569, 1029, 2112, 2824, 3519, 3636, 3644
AlwI GGATC 7 cut(s) 130, 213, 683, 1516, 2618, 3350, 3355
AlwNI CAGNNNCTG 3 cut(s) 1991, 2509, 2710
Ama87I CYCGRG 1 cut(s) 1156
Aor13HI TCCGGA 1 cut(s) 874
ApeKI GCWGC 9 cut(s) 40, 197, 1562, 1792, 1902, 1919, 2084, 2369, 3661
ApoI RAATTY 6 cut(s) 77, 346, 1339, 1849, 3466, 3646
ArsI GACNNNNNNTTYG 2 cut(s) 3614, 3646
AseI ATTAAT 1 cut(s) 3501
Asp700I GAANNNNTTC 2 cut(s) 594, 2491
Asp718I GGTACC 3 cut(s) 1126, 1447, 2988
AspA2I CCTAGG 1 cut(s) 2999
AspLEI GCGC 3 cut(s) 833, 1216, 1691
AsuC2I CCSGG 4 cut(s) 253, 1131, 2981, 3396
AvaI CYCGRG 1 cut(s) 1156
AvaII GGWCC 9 cut(s) 730, 790, 1529, 1636, 2011, 2762, 3029, 3065, 3245
AvrII CCTAGG 1 cut(s) 2999
AxyI CCTNAGG 1 cut(s) 511
BaeGI GKGCMC 3 cut(s) 820, 1618, 3147
BanI GGYRCC 5 cut(s) 248, 1126, 1447, 2988, 3144
BanII GRGCYC 2 cut(s) 2659, 3734
BarI GAAGNNNNNNTAC 6 cut(s) 454, 486, 1440, 1472, 2205, 2237
BauI CACGAG 2 cut(s) 2088, 3060
BbsI GAAGAC 4 cut(s) 872, 897, 1087, 1448
Bbv12I GWGCWC 4 cut(s) 414, 2659, 3433, 3734
BbvCI CCTCAGC 1 cut(s) 924
BbvI GCAGC 9 cut(s) 52, 184, 1574, 1804, 1889, 1906, 2096, 2356, 3673
BceAI ACGGC 2 cut(s) 147, 1227
BclI TGATCA 2 cut(s) 1200, 2044
BcnI CCSGG 4 cut(s) 253, 1131, 2981, 3396
BcoDI GTCTC 9 cut(s) 356, 567, 569, 1029, 2112, 2824, 3519, 3636, 3644
BfaI CTAG 6 cut(s) 584, 1643, 2337, 2964, 3000, 3195
BfmI CTRYAG 5 cut(s) 682, 1347, 1560, 1719, 2222
BfuAI ACCTGC 1 cut(s) 2146
BlnI CCTAGG 1 cut(s) 2999
Bme18I GGWCC 9 cut(s) 730, 790, 1529, 1636, 2011, 2762, 3029, 3065, 3245
BmeT110I CYCGRG 1 cut(s) 1156
BmrI ACTGGG 3 cut(s) 736, 1674, 2415
BmsI GCATC 8 cut(s) 44, 313, 868, 940, 1153, 1481, 2182, 2608
BmuI ACTGGG 3 cut(s) 736, 1674, 2415
BoxI GACNNNNGTC 1 cut(s) 735
BpiI GAAGAC 4 cut(s) 872, 897, 1087, 1448
Bpu10I CCTNAGC 2 cut(s) 812, 924
BpuEI CTTGAG 3 cut(s) 72, 1962, 3456
BpuMI CCSGG 4 cut(s) 253, 1131, 2981, 3396
BsaI GGTCTC 1 cut(s) 3644
BsaWI WCCGGW 1 cut(s) 874
BsaXI ACNNNNNCTCC 2 cut(s) 1152, 1182
Bse118I RCCGGY 2 cut(s) 245, 2289
Bse1I ACTGG 6 cut(s) 370, 731, 958, 1669, 2421, 2532
Bse21I CCTNAGG 1 cut(s) 511
Bse3DI GCAATG 1 cut(s) 1338
BseAI TCCGGA 1 cut(s) 874
BseGI GGATG 9 cut(s) 883, 1171, 1175, 2173, 2195, 3292, 3575, 3589, 3748
BseMI GCAATG 1 cut(s) 1338
BseNI ACTGG 6 cut(s) 370, 731, 958, 1669, 2421, 2532
BseRI GAGGAG 4 cut(s) 207, 1235, 2339, 3081
BseSI GKGCMC 3 cut(s) 820, 1618, 3147
BseXI GCAGC 9 cut(s) 52, 184, 1574, 1804, 1889, 1906, 2096, 2356, 3673
BseYI CCCAGC 2 cut(s) 442, 2314
BsgI GTGCAG 1 cut(s) 1888
Bsh1236I CGCG 4 cut(s) 203, 276, 1872, 2653
Bsh1285I CGRYCG 1 cut(s) 181
BshNI GGYRCC 5 cut(s) 248, 1126, 1447, 2988, 3144
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 4 cut(s) 414, 2659, 3433, 3734
BsiHKCI CYCGRG 1 cut(s) 1156
BsiSI CCGG 8 cut(s) 44, 246, 252, 875, 1130, 2290, 2981, 3395
BsmAI GTCTC 9 cut(s) 356, 567, 569, 1029, 2112, 2824, 3519, 3636, 3644
BsmI GAATGC 3 cut(s) 608, 1326, 1474
Bso31I GGTCTC 1 cut(s) 3644
BsoBI CYCGRG 1 cut(s) 1156
Bsp1286I GDGCHC 7 cut(s) 414, 820, 1618, 2659, 3147, 3433, 3734
Bsp13I TCCGGA 1 cut(s) 874
Bsp1407I TGTACA 1 cut(s) 2511
Bsp19I CCATGG 1 cut(s) 3106
BspEI TCCGGA 1 cut(s) 874
BspFNI CGCG 4 cut(s) 203, 276, 1872, 2653
BspHI TCATGA 1 cut(s) 3352
BspMAI CTGCAG 1 cut(s) 1564
BspMI ACCTGC 1 cut(s) 2146
BspPI GGATC 7 cut(s) 130, 213, 683, 1516, 2618, 3350, 3355
BspT107I GGYRCC 5 cut(s) 248, 1126, 1447, 2988, 3144
BspTNI GGTCTC 1 cut(s) 3644
BsrDI GCAATG 1 cut(s) 1338
BsrFI RCCGGY 2 cut(s) 245, 2289
BsrGI TGTACA 1 cut(s) 2511
BsrI ACTGG 6 cut(s) 370, 731, 958, 1669, 2421, 2532
BssAI RCCGGY 2 cut(s) 245, 2289
BssNAI GTATAC 1 cut(s) 66
BssSI CACGAG 2 cut(s) 2088, 3060
BssT1I CCWWGG 4 cut(s) 1525, 2014, 2999, 3106
Bst1107I GTATAC 1 cut(s) 66
Bst2BI CACGAG 2 cut(s) 2088, 3060
Bst4CI ACNGT 9 cut(s) 52, 147, 578, 614, 1048, 1552, 2106, 2511, 2796
Bst6I CTCTTC 2 cut(s) 354, 2899
BstAPI GCANNNNNTGC 2 cut(s) 1789, 2090
BstAUI TGTACA 1 cut(s) 2511
BstC8I GCNNGC 9 cut(s) 414, 448, 543, 820, 2095, 2655, 2854, 3230, 3562
BstDSI CCRYGG 4 cut(s) 201, 2273, 2627, 3106
BstEII GGTNACC 1 cut(s) 2885
BstENI CCTNNNNNAGG 1 cut(s) 1539
BstF5I GGATG 9 cut(s) 883, 1171, 1175, 2173, 2195, 3292, 3575, 3589, 3748
BstFNI CGCG 4 cut(s) 203, 276, 1872, 2653
BstHHI GCGC 3 cut(s) 833, 1216, 1691
BstMAI GTCTC 9 cut(s) 356, 567, 569, 1029, 2112, 2824, 3519, 3636, 3644
BstMCI CGRYCG 1 cut(s) 181
BstNSI RCATGY 2 cut(s) 1199, 1911
BstPAI GACNNNNGTC 1 cut(s) 735
BstPI GGTNACC 1 cut(s) 2885
BstSFI CTRYAG 5 cut(s) 682, 1347, 1560, 1719, 2222
BstSLI GKGCMC 3 cut(s) 820, 1618, 3147
BstUI CGCG 4 cut(s) 203, 276, 1872, 2653
BstV1I GCAGC 9 cut(s) 52, 184, 1574, 1804, 1889, 1906, 2096, 2356, 3673
BstV2I GAAGAC 4 cut(s) 872, 897, 1087, 1448
BstX2I RGATCY 2 cut(s) 688, 3347
BstXI CCANNNNNNTGG 3 cut(s) 119, 3113, 3587
BstYI RGATCY 2 cut(s) 688, 3347
BstZ17I GTATAC 1 cut(s) 66
Bsu36I CCTNAGG 1 cut(s) 511
BtgI CCRYGG 4 cut(s) 201, 2273, 2627, 3106
BtgZI GCGATG 2 cut(s) 21, 148
BtsCI GGATG 9 cut(s) 883, 1171, 1175, 2173, 2195, 3292, 3575, 3589, 3748
BtsI GCAGTG 1 cut(s) 1834
BtsIMutI CAGTG 6 cut(s) 746, 1044, 1288, 1834, 2338, 2539
BveI ACCTGC 1 cut(s) 2146
Cac8I GCNNGC 9 cut(s) 414, 448, 543, 820, 2095, 2655, 2854, 3230, 3562
CaiI CAGNNNCTG 3 cut(s) 1991, 2509, 2710
CciI TCATGA 1 cut(s) 3352
CfoI GCGC 3 cut(s) 833, 1216, 1691
Cfr10I RCCGGY 2 cut(s) 245, 2289
Cfr42I CCGCGG 1 cut(s) 204
CseI GACGC 2 cut(s) 282, 439
CsiI ACCWGGT 5 cut(s) 534, 904, 1450, 1743, 3238
Csp6I GTAC 9 cut(s) 955, 1127, 1153, 1305, 1448, 1958, 2512, 2792, 2989
CviQI GTAC 9 cut(s) 955, 1127, 1153, 1305, 1448, 1958, 2512, 2792, 2989
DraI TTTAAA 1 cut(s) 3201
DraIII CACNNNGTG 3 cut(s) 538, 3242, 3398
EaeI YGGCCR 4 cut(s) 54, 137, 330, 3369
Eam1104I CTCTTC 2 cut(s) 354, 2899
EarI CTCTTC 2 cut(s) 354, 2899
Ecl136II GAGCTC 2 cut(s) 2657, 3732
Eco130I CCWWGG 4 cut(s) 1525, 2014, 2999, 3106
Eco147I AGGCCT 1 cut(s) 3389
Eco24I GRGCYC 2 cut(s) 2659, 3734
Eco31I GGTCTC 1 cut(s) 3644
Eco32I GATATC 1 cut(s) 3334
Eco47I GGWCC 9 cut(s) 730, 790, 1529, 1636, 2011, 2762, 3029, 3065, 3245
Eco53kI GAGCTC 2 cut(s) 2657, 3732
Eco57I CTGAAG 4 cut(s) 734, 1487, 3117, 3611
Eco81I CCTNAGG 1 cut(s) 511
Eco88I CYCGRG 1 cut(s) 1156
Eco91I GGTNACC 1 cut(s) 2885
EcoICRI GAGCTC 2 cut(s) 2657, 3732
EcoNI CCTNNNNNAGG 1 cut(s) 1539
EcoO109I RGGNCCY 3 cut(s) 730, 790, 2762
EcoO65I GGTNACC 1 cut(s) 2885
EcoRI GAATTC 2 cut(s) 77, 3466
EcoRV GATATC 1 cut(s) 3334
EcoT14I CCWWGG 4 cut(s) 1525, 2014, 2999, 3106
EcoT22I ATGCAT 3 cut(s) 328, 1474, 2175
EcoT38I GRGCYC 2 cut(s) 2659, 3734
ErhI CCWWGG 4 cut(s) 1525, 2014, 2999, 3106
FauNDI CATATG 2 cut(s) 1898, 2446
FbaI TGATCA 2 cut(s) 1200, 2044
FblI GTMKAC 2 cut(s) 65, 1973
FokI GGATG 9 cut(s) 890, 1158, 1162, 2160, 2182, 3299, 3562, 3596, 3735
FriOI GRGCYC 2 cut(s) 2659, 3734
FspBI CTAG 6 cut(s) 584, 1643, 2337, 2964, 3000, 3195
FspI TGCGCA 1 cut(s) 1690
GlaI GCGC 3 cut(s) 832, 1215, 1690
GsaI CCCAGC 2 cut(s) 446, 2318
HapII CCGG 8 cut(s) 44, 246, 252, 875, 1130, 2290, 2981, 3395
HgaI GACGC 2 cut(s) 282, 439
HhaI GCGC 3 cut(s) 833, 1216, 1691
Hin6I GCGC 3 cut(s) 831, 1214, 1689
HinP1I GCGC 3 cut(s) 831, 1214, 1689
HincII GTYRAC 1 cut(s) 2727
HindII GTYRAC 1 cut(s) 2727
HindIII AAGCTT 1 cut(s) 1309
HpaII CCGG 8 cut(s) 44, 246, 252, 875, 1130, 2290, 2981, 3395
Hpy166II GTNNAC 9 cut(s) 66, 581, 1532, 1636, 1974, 2702, 2727, 3065, 3613
Hpy8I GTNNAC 9 cut(s) 66, 581, 1532, 1636, 1974, 2702, 2727, 3065, 3613
Hpy99I CGWCG 4 cut(s) 62, 133, 455, 1440
HpyCH4III ACNGT 9 cut(s) 52, 147, 578, 614, 1048, 1552, 2106, 2511, 2796
HpyCH4IV ACGT 4 cut(s) 171, 1144, 1266, 1438
HpySE526I ACGT 4 cut(s) 171, 1144, 1266, 1438
HspAI GCGC 3 cut(s) 831, 1214, 1689
Kpn2I TCCGGA 1 cut(s) 874
KpnI GGTACC 3 cut(s) 1130, 1451, 2992
Ksp22I TGATCA 2 cut(s) 1200, 2044
KspI CCGCGG 1 cut(s) 204
LmnI GCTCC 7 cut(s) 194, 409, 1328, 2849, 3094, 3116, 3428
Lsp1109I GCAGC 9 cut(s) 52, 184, 1574, 1804, 1889, 1906, 2096, 2356, 3673
LweI GCATC 8 cut(s) 44, 313, 868, 940, 1153, 1481, 2182, 2608
MabI ACCWGGT 5 cut(s) 534, 904, 1450, 1743, 3238
MaeI CTAG 6 cut(s) 584, 1643, 2337, 2964, 3000, 3195
MaeII ACGT 4 cut(s) 171, 1144, 1266, 1438
MaeIII GTNAC 7 cut(s) 225, 1421, 1552, 2125, 2233, 2389, 2885
MfeI CAATTG 1 cut(s) 1566
MflI RGATCY 2 cut(s) 688, 3347
MhlI GDGCHC 7 cut(s) 414, 820, 1618, 2659, 3147, 3433, 3734
MlyI GAGTC 5 cut(s) 1031, 1979, 2122, 2241, 3566
MmeI TCCRAC 6 cut(s) 602, 1171, 2223, 2524, 3080, 3160
Mph1103I ATGCAT 3 cut(s) 328, 1474, 2175
MroI TCCGGA 1 cut(s) 874
MroXI GAANNNNTTC 2 cut(s) 594, 2491
MspA1I CMGCKG 4 cut(s) 203, 335, 2314, 3495
MspI CCGG 8 cut(s) 44, 246, 252, 875, 1130, 2290, 2981, 3395
MssI GTTTAAAC 1 cut(s) 3201
MunI CAATTG 1 cut(s) 1566
Mva1269I GAATGC 3 cut(s) 608, 1326, 1474
MvnI CGCG 4 cut(s) 203, 276, 1872, 2653
NciI CCSGG 4 cut(s) 253, 1131, 2981, 3396
NcoI CCATGG 1 cut(s) 3106
NdeI CATATG 2 cut(s) 1898, 2446
NmuCI GTSAC 5 cut(s) 225, 1552, 2125, 2233, 2389
NsbI TGCGCA 1 cut(s) 1690
NsiI ATGCAT 3 cut(s) 328, 1474, 2175
NspI RCATGY 2 cut(s) 1199, 1911
PagI TCATGA 1 cut(s) 3352
PceI AGGCCT 1 cut(s) 3389
PciI ACATGT 2 cut(s) 1195, 1907
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 230
PctI GAATGC 3 cut(s) 608, 1326, 1474
PdmI GAANNNNTTC 2 cut(s) 594, 2491
PfeI GAWTC 9 cut(s) 5, 95, 152, 529, 1278, 2540, 2613, 3460, 3598
PflFI GACNNNGTC 1 cut(s) 145
PflMI CCANNNNNTGG 2 cut(s) 2957, 3062
PfoI TCCNGGA 1 cut(s) 3445
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 5 cut(s) 1030, 1978, 2122, 2240, 3566
PmeI GTTTAAAC 1 cut(s) 3201
PpsI GAGTC 5 cut(s) 1030, 1978, 2122, 2240, 3566
PpuMI RGGWCCY 3 cut(s) 730, 790, 2762
PscI ACATGT 2 cut(s) 1195, 1907
PshAI GACNNNNGTC 1 cut(s) 735
PshBI ATTAAT 1 cut(s) 3501
PsiI TTATAA 1 cut(s) 2438
Psp124BI GAGCTC 2 cut(s) 2659, 3734
Psp5II RGGWCCY 3 cut(s) 730, 790, 2762
PspEI GGTNACC 1 cut(s) 2885
PspFI CCCAGC 2 cut(s) 442, 2314
PspPPI RGGWCCY 3 cut(s) 730, 790, 2762
PstI CTGCAG 1 cut(s) 1564
PstNI CAGNNNCTG 3 cut(s) 1991, 2509, 2710
PsuI RGATCY 2 cut(s) 688, 3347
PsyI GACNNNGTC 1 cut(s) 145
PvuI CGATCG 1 cut(s) 181
PvuII CAGCTG 1 cut(s) 2314
RsaI GTAC 9 cut(s) 956, 1128, 1154, 1306, 1449, 1959, 2513, 2793, 2990
RsaNI GTAC 9 cut(s) 955, 1127, 1153, 1305, 1448, 1958, 2512, 2792, 2989
SacI GAGCTC 2 cut(s) 2659, 3734
SacII CCGCGG 1 cut(s) 204
SchI GAGTC 5 cut(s) 1031, 1979, 2122, 2241, 3566
SduI GDGCHC 7 cut(s) 414, 820, 1618, 2659, 3147, 3433, 3734
SexAI ACCWGGT 5 cut(s) 534, 904, 1450, 1743, 3238
SfaNI GCATC 8 cut(s) 44, 313, 868, 940, 1153, 1481, 2182, 2608
SfcI CTRYAG 5 cut(s) 682, 1347, 1560, 1719, 2222
Sfr303I CCGCGG 1 cut(s) 204
SgrBI CCGCGG 1 cut(s) 204
SinI GGWCC 9 cut(s) 730, 790, 1529, 1636, 2011, 2762, 3029, 3065, 3245
SmlI CTYRAG 3 cut(s) 87, 1977, 3471
SmoI CTYRAG 3 cut(s) 87, 1977, 3471
SseBI AGGCCT 1 cut(s) 3389
SspMI CTAG 6 cut(s) 584, 1643, 2337, 2964, 3000, 3195
SstI GAGCTC 2 cut(s) 2659, 3734
StuI AGGCCT 1 cut(s) 3389
StyI CCWWGG 4 cut(s) 1525, 2014, 2999, 3106
TaaI ACNGT 9 cut(s) 52, 147, 578, 614, 1048, 1552, 2106, 2511, 2796
TaiI ACGT 4 cut(s) 174, 1147, 1269, 1441
TaqI TCGA 9 cut(s) 105, 437, 600, 993, 1318, 1435, 2757, 2914, 3570
TatI WGTACW 2 cut(s) 1304, 2511
TauI GCSGC 2 cut(s) 203, 335
TfiI GAWTC 9 cut(s) 5, 95, 152, 529, 1278, 2540, 2613, 3460, 3598
TscAI CASTG 6 cut(s) 746, 1051, 1288, 1834, 2338, 2539
TseFI GTSAC 5 cut(s) 225, 1552, 2125, 2233, 2389
TseI GCWGC 9 cut(s) 40, 197, 1562, 1792, 1902, 1919, 2084, 2369, 3661
Tsp45I GTSAC 5 cut(s) 225, 1552, 2125, 2233, 2389
TspGWI ACGGA 2 cut(s) 422, 2644
TspRI CASTG 6 cut(s) 746, 1051, 1288, 1834, 2338, 2539
Tth111I GACNNNGTC 1 cut(s) 145
Van91I CCANNNNNTGG 2 cut(s) 2957, 3062
VpaK11BI GGWCC 9 cut(s) 730, 790, 1529, 1636, 2011, 2762, 3029, 3065, 3245
VspI ATTAAT 1 cut(s) 3501
XagI CCTNNNNNAGG 1 cut(s) 1539
XapI RAATTY 6 cut(s) 77, 346, 1339, 1849, 3466, 3646
XceI RCATGY 2 cut(s) 1199, 1911
XcmI CCANNNNNNNNNTGG 4 cut(s) 1532, 3176, 3236, 3263
XmaJI CCTAGG 1 cut(s) 2999
XmiI GTMKAC 2 cut(s) 65, 1973
XmnI GAANNNNTTC 2 cut(s) 594, 2491
XspI CTAG 6 cut(s) 584, 1643, 2337, 2964, 3000, 3195
Zsp2I ATGCAT 3 cut(s) 328, 1474, 2175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.