Rh7BG229800

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009459
Physical Location & Seq
Reverse (-)
20053298 .. 20065301
12004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG229800.1

Sequence Viewer

Length: 3741 bp
ATGGAATCGCAGCCGAACAACCTCTTCGACACGGCGTCGCAGCCGGACACGGCCACCGACGCCTACACCTTCCTCGAATTCAACACCCAGGGCGAGGACTTCGACTACCCGGAGTTTCGCGACCCGATTCGCTCCCCGGTGGCCTGGCCCACGCCGTCCGATTCCTTATCGGACCCTGCCGACCGCGACCGCGGCGGAGGAGGAGGTGGTGGTGGCTCCGACCACCAGTCTGATGCGTCGCCTGTTTCGGCTGCCCCGGGCAGCGCCACGAAGGCCCGGGCCGGTGGCTCGGGAAGTAGCGGCGGGAATAATAATCAGGTGGTTGACGCCCTAGCGTCTGGGATGAGTGTGCTGAACTTTGAGGACACTGGGGACGATGATAGCTATGAGTTTGGAAAGGGGGATTTCACTGAGCATGCGTGCCGGTACTGCGGCGTGTCGAACCCGGCGTGTGTTGTGAGGTGTAACGTGCCGTCGTGCCGGAAGTGGTTTTGTAATTCCAGGGGGAACACGTCCGGCTCTCATATTGTGAATCACCTGGTTCGTGCAAAACACAAGGAAGTCTGTCTTCATAAAGATAGCCCATTAGGAGAAACAATCCTTGAGTGCTACAACTGTGGATGCCGCAATGTATTCCTTCTTGGATTTATTTCAGCCAAGACAGAGAGTGTTGTCGTTCTTCTCTGTAGGGAACCCTGCTTAAGTGTTAATGCATTGAAGGATATGAACTGGGACCTGAGCCAGTGGTGTCCCCTGATTGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGTCCCCTCCGAACAGGAGCAGCTGAGGGCACGCCAGATTAGTGCTCAACAAATAAACAAGGTAGAAGAACTCTGGAAGTCAAATCCTGATGCTTCCCTTGAAGATCTTGAGAAGCCTGGTGTGGATGATGAACCCCAACCTGTGGCCGTAAAGTATGAGGATGCATACCAGTATCAAAATGTGTTTGCACCACTTATCAAGCTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGCAAGGACAATCTCACCATTAGGTGGGATATTGGTCTTAACAAGAAGCGTGTCGCTTATTTTATTTTTCCAAAGGAGGACAATGAGATTCGTCTTGTACCTGGTGATGAGCTGCGGCTTCGTTATTCTGGGGATGCAGCTCATCCAGCATGGCAGTCTGTGGGACATGTGATCAAGTTAACTGCACAAGAAGAGGTTGCACTCGAACTTCGTGCTAGTCAGGGAGTTCCAGCTGATGTGAACCATGGCTTCAGCGTTGATTTCGTGTGGAAGAGTACAAGTTTTGATCGTATGCAGGGAGCCATGAAAGCTTTTGCAGTGGATGAGACAAGTGTTAGTGGGTATATTTACCATCACTTGTTAGGCCATGAAGTAGAGGTTCAGATGGTCCGTAATACACTGCCTCGTCGTTTTGGTGCACCTGGTCTGCCAGAGCTCAATGCATCTCAGGTTTTTGCTGTGAAAAGTGTCCTTCAGAAGCCTATAAGTTTGATTCAAGGTCCACCTGGCACAGGAAAAACCGTCACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGGCAGGGACAGGTCTTGGTTTGCGCTCCTAGTAATGTGGCTGTAGACCAACTAGCTGAAAAAATAAGTGCAACTGGGCTGAAGGTTGTTAGACTTTGTGCAAAATCAAGGGAAGCTGTCAGCTCTCCTGTTGAGCATTTAACCCTTCACTATCAGGTTCGTCATCTTGACACATCAGAAAAGAGTGAACTTCACAAGTTACAGCAATTGAAGGATGAGCAAGGCGAATTGTCAAGTAGTGATGAGAAAAAGTACAAAGCGCTGAAGCGGGCAACTGAAAGAGAGATTTCTCAGAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGGGATCCTCGATTGGCGAATTTTAGGTTCCGTCAGGTACTTATTGATGAGTCTACTCAGGCAACAGAGCCTGAATGCCTTATTCCTTTGGTACTAGGGGCAAAGCAGGTTGTTTTGGTTGGTGATCACTGCCAGCTTGGTCCAGTCATTATGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTTTTTGAACGCCTCGTTTTACTTGGTGTGAAACCAATTAGGTTGCAGGTCCAATACAGAATGCATCCAGCCTTGTCAGAATTTCCTTCCAACAGCTTCTATGAGGGAACACTCCAAAATGGCGTCACCATCAATGAAAGGCAGTCAACGGGAATTGATTTTCCATGGCCAGTTCCCAATCGTCCCATGTTCTTTTATGTTCAGATGGGGCAAGAGGAGATAAGTGCTAGCGGAACATCTTACCTGAACAGAACTGAAGCTGCTAATGTTGAAAAGATTGTCACAACATTCTTAAGGAGTGGTGTCGTCCCTAGTCAGATCGGGGTGATAACACCATATGAGGGACAGCGAGCATATATTGTGAACTATATGTCAAGAAATGGTGCTCTCAGGCAGCAACTTTATAAGGAAATAGAGGTTGCAAGTGTAGATTCATTTCAAGGAAGGGAAAAAGATTATATCATCCTTTCATGTGTGAGGAGTAATGAGCATCAGGGCATTGGATTCCTTAACGACCCTCGGAGGCTCAATGTTGCCCTAACACGTGCTCGGTATGGTATTGTTATTCTGGGGAACCCTAAAGTTCTAAGTAAACAGCCCTTGTGGAATAGCTTGTTGACGCACTACAAGGAACATGAGTGCTTGGTCGAAGGTCCTCTAAATAACTTGAAGCAGAGTATGGTTCAATTTCCAAAGCCCAAAAAGATCTACAATGACCGGCGTCTGTTCTTTGGTGGTGGTCCTGGAGTTGTCCCAAGTGATAGTTATGGGTCTATTCCCCCATCTGGTCAGAGTGCTGATAGAAGAAGTGGTCGTGGTAGGGGCTCTTACCTACCTCCTGGTCCACCGAATGGTACTCACAAGCCTGGGGTACATCCTGCTGGATATCCAATGCCTCGGGCACCACTTGCACCCTTTCATGGTGGTCCCCTTTCTCAACCCTATGCTATTCCAACTCGTGGAGCCGTGCATGGACCAGTAGGAGCTGTTCCTCATGTCCCTCAACCTGGAAGTCGAGGTTTTGGAGCTGGACGTGGCAGTGCTGGTGCTCCTATTGGCAGTCATCTCCAACACCAGCAAGGCACACAGCAGAATGTAGGAAATCTGGGGTCTAACTTCAACTTTCCTGCTCTGGAAAATCCTAGTAGTCAGCCATCTGTGGGCGGTCCATTATCTCAACCTGGATTTGTCAATAATATGCCAGTTCAAGGGCCAAGCCAAACATTTCGTGATGGATTTTCCATGGCGGGCATGTCTCAGGAGTTCTTGGGTGATGACTTTAAAAGTCAGGGATCTCATGTTCCTTATAATGTTGCTGACTTTTCCACTCAGGCTTCTCAAAGTGGATATGCTGTTGATTATGTTACACAAGGAGCACAAGGAGGATTTCCAAGCAATTTTATAAACCAGAATTCTCAAGCAGGGTACTCCCGATTTGGTACAGGGAATGACTTCATGTCTCAGGACTACATGGCTCATGGTACACAAGGTCTATTCACCCAGGTGGGCTACAATGACCCATCACAGGATGATGGATCGCAGAACCACTATGGAGTGGGGAATGCTAATGCGCTTCAGTCTCAGGTTTCTTCTCATACAAACTGTTTGATATATAATTATCATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

1246

Amino Acids

136.02

Weight (kDa)

6.3

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 139 - 292 8.7e-73 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 344 - 433 1.8e-26 RNA helicase UPF1, 1B domain
AAA_11 PF13086 489 - 609 2e-23 AAA domain
AAA_30 PF13604 489 - 690 5.7e-14 AAA domain
ResIII PF04851 489 - 559 4.8e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 496 - 690 3.4e-11 AAA domain
AAA_11 PF13086 591 - 692 9.7e-29 AAA domain
AAA_12 PF13087 701 - 897 1.1e-61 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2517, 3420, 3515
Acc36I ACCTGC 2 cut(s) 2020, 2149
AccB1I GGYRCC 1 cut(s) 3013
AccB7I CCANNNNNTGG 4 cut(s) 934, 1056, 2963, 3071
AccI GTMKAC 2 cut(s) 1638, 1976
AccII CGCG 3 cut(s) 120, 186, 192
AclWI GGATC 4 cut(s) 1922, 1935, 3412, 3655
AcoI YGGCCR 3 cut(s) 51, 936, 2279
AcsI RAATTY 4 cut(s) 77, 1942, 2192, 3523
AcuI CTGAAG 6 cut(s) 1267, 1490, 1694, 1877, 2388, 3671
AcvI CACGTG 1 cut(s) 2657
AcyI GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
AfeI AGCGCT 1 cut(s) 1854
AflII CTTAAG 2 cut(s) 700, 2404
AflIII ACRYGT 4 cut(s) 510, 1198, 1910, 2654
AhdI GACNNNNNGTC 3 cut(s) 34, 226, 3568
AjiI CACGTC 2 cut(s) 513, 3146
AleI CACNNNNGTG 1 cut(s) 3614
AloI GAACNNNNNNTCC 2 cut(s) 3396, 3428
Alw21I GWGCWC 7 cut(s) 838, 1453, 1470, 2500, 2662, 3163, 3490
Alw26I GTCTC 5 cut(s) 1352, 2115, 3372, 3576, 3696
Alw44I GTGCAC 1 cut(s) 1449
AlwI GGATC 4 cut(s) 1922, 1935, 3412, 3655
AlwNI CAGNNNCTG 1 cut(s) 1994
Ama87I CYCGRG 4 cut(s) 256, 276, 289, 3009
Aor51HI AGCGCT 1 cut(s) 1854
AoxI GGCC 9 cut(s) 51, 141, 146, 273, 279, 936, 1396, 2279, 3323
ApaLI GTGCAC 1 cut(s) 1449
ApoI RAATTY 4 cut(s) 77, 1942, 2192, 3523
Asp700I GAANNNNTTC 1 cut(s) 3563
AspLEI GCGC 4 cut(s) 266, 1619, 1855, 3685
AsuC2I CCSGG 7 cut(s) 110, 137, 257, 258, 277, 278, 446
AsuHPI GGTGA 8 cut(s) 527, 1039, 1148, 2057, 2230, 2449, 3395, 3601
AsuNHI GCTAGC 1 cut(s) 2339
AvaI CYCGRG 4 cut(s) 256, 276, 289, 3009
BaeGI GKGCMC 3 cut(s) 823, 1453, 3016
BalI TGGCCA 1 cut(s) 2281
BamHI GGATCC 1 cut(s) 1927
BanI GGYRCC 1 cut(s) 3013
BanII GRGCYC 2 cut(s) 1470, 2939
BauI CACGAG 2 cut(s) 2091, 3069
BbrPI CACGTG 1 cut(s) 2657
BbsI GAAGAC 1 cut(s) 560
Bbv12I GWGCWC 7 cut(s) 838, 1453, 1470, 2500, 2662, 3163, 3490
BbvCI CCTCAGC 1 cut(s) 815
BccI CCATC 9 cut(s) 1392, 1411, 2249, 2311, 2902, 3274, 3338, 3638, 3640
BceAI ACGGC 6 cut(s) 48, 66, 139, 457, 923, 3062
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BclI TGATCA 2 cut(s) 1203, 2047
BcnI CCSGG 7 cut(s) 110, 137, 257, 258, 277, 278, 446
BcoDI GTCTC 5 cut(s) 1352, 2115, 3372, 3576, 3696
BfaI CTAG 8 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424, 3255
BfmI CTRYAG 3 cut(s) 685, 1563, 1635
BfoI RGCGCY 2 cut(s) 267, 1856
BfrI CTTAAG 2 cut(s) 700, 2404
BfuAI ACCTGC 2 cut(s) 2020, 2149
BglI GCCNNNNNGGC 1 cut(s) 272
BglII AGATCT 2 cut(s) 895, 2817
BmeRI GACNNNNNGTC 3 cut(s) 34, 226, 3568
BmeT110I CYCGRG 4 cut(s) 256, 276, 289, 3009
BmgBI CACGTC 2 cut(s) 513, 3146
BmrI ACTGGG 4 cut(s) 378, 739, 1677, 2101
BmsI GCATC 8 cut(s) 223, 611, 871, 943, 1156, 1484, 2185, 2611
BmtI GCTAGC 1 cut(s) 2343
BmuI ACTGGG 4 cut(s) 378, 739, 1677, 2101
BoxI GACNNNNGTC 1 cut(s) 2832
BpiI GAAGAC 1 cut(s) 560
BpmI CTGGAG 1 cut(s) 2877
Bpu10I CCTNAGC 2 cut(s) 737, 815
BpuEI CTTGAG 3 cut(s) 623, 920, 3513
BpuMI CCSGG 7 cut(s) 110, 137, 257, 258, 277, 278, 446
BsaAI YACGTR 1 cut(s) 2657
BsaHI GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
Bse118I RCCGGY 3 cut(s) 281, 423, 2829
Bse3DI GCAATG 1 cut(s) 634
BseMI GCAATG 1 cut(s) 634
BseRI GAGGAG 4 cut(s) 213, 216, 2342, 2605
BseSI GKGCMC 3 cut(s) 823, 1453, 3016
BseYI CCCAGC 2 cut(s) 1922, 2096
BsgI GTGCAG 1 cut(s) 1200
Bsh1236I CGCG 3 cut(s) 120, 186, 192
Bsh1285I CGRYCG 2 cut(s) 184, 190
BshFI GGCC 9 cut(s) 53, 143, 148, 275, 281, 938, 1398, 2281, 3325
BshNI GGYRCC 1 cut(s) 3013
BsiEI CGRYCG 2 cut(s) 184, 190
BsiHKAI GWGCWC 7 cut(s) 838, 1453, 1470, 2500, 2662, 3163, 3490
BsiHKCI CYCGRG 4 cut(s) 256, 276, 289, 3009
BsmAI GTCTC 5 cut(s) 1352, 2115, 3372, 3576, 3696
BsmI GAATGC 3 cut(s) 2003, 2178, 3679
BsnI GGCC 9 cut(s) 53, 143, 148, 275, 281, 938, 1398, 2281, 3325
BsoBI CYCGRG 4 cut(s) 256, 276, 289, 3009
Bsp143I GATC 9 cut(s) 895, 1203, 1318, 1927, 2047, 2430, 2817, 3404, 3647
Bsp19I CCATGG 3 cut(s) 1276, 2276, 3354
Bsp68I TCGCGA 1 cut(s) 120
BspANI GGCC 9 cut(s) 53, 143, 148, 275, 281, 938, 1398, 2281, 3325
BspFNI CGCG 3 cut(s) 120, 186, 192
BspMAI CTGCAG 1 cut(s) 1567
BspMI ACCTGC 2 cut(s) 2020, 2149
BspOI GCTAGC 1 cut(s) 2343
BspPI GGATC 4 cut(s) 1922, 1935, 3412, 3655
BspT107I GGYRCC 1 cut(s) 3013
BspTI CTTAAG 2 cut(s) 700, 2404
BsrDI GCAATG 1 cut(s) 634
BsrFI RCCGGY 3 cut(s) 281, 423, 2829
BssAI RCCGGY 3 cut(s) 281, 423, 2829
BssMI GATC 9 cut(s) 895, 1203, 1318, 1927, 2047, 2430, 2817, 3404, 3647
BssNI GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
BssSI CACGAG 2 cut(s) 2091, 3069
BssT1I CCWWGG 3 cut(s) 1276, 2276, 3354
Bst2BI CACGAG 2 cut(s) 2091, 3069
Bst4CI ACNGT 3 cut(s) 617, 1555, 3716
Bst6I CTCTTC 3 cut(s) 29, 1218, 1298
BstACI GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
BstAFI CTTAAG 2 cut(s) 700, 2404
BstAPI GCANNNNNTGC 2 cut(s) 2093, 3020
BstBAI YACGTR 1 cut(s) 2657
BstC8I GCNNGC 9 cut(s) 417, 421, 823, 1863, 2057, 2103, 2341, 2463, 3361
BstDSI CCRYGG 4 cut(s) 190, 1276, 2276, 3354
BstENI CCTNNNNNAGG 1 cut(s) 1542
BstFNI CGCG 3 cut(s) 120, 186, 192
BstH2I RGCGCY 2 cut(s) 267, 1856
BstHHI GCGC 4 cut(s) 266, 1619, 1855, 3685
BstKTI GATC 9 cut(s) 898, 1206, 1321, 1930, 2050, 2433, 2820, 3407, 3650
BstMAI GTCTC 5 cut(s) 1352, 2115, 3372, 3576, 3696
BstMBI GATC 9 cut(s) 895, 1203, 1318, 1927, 2047, 2430, 2817, 3404, 3647
BstMCI CGRYCG 2 cut(s) 184, 190
BstMWI GCNNNNNNNGC 9 cut(s) 59, 192, 272, 429, 1178, 1340, 2093, 2102, 3020
BstNSI RCATGY 4 cut(s) 419, 1202, 1914, 3367
BstPAI GACNNNNGTC 1 cut(s) 2832
BstSFI CTRYAG 3 cut(s) 685, 1563, 1635
BstSLI GKGCMC 3 cut(s) 823, 1453, 3016
BstUI CGCG 3 cut(s) 120, 186, 192
BstV2I GAAGAC 1 cut(s) 560
BstX2I RGATCY 4 cut(s) 895, 1927, 2817, 3404
BstYI RGATCY 4 cut(s) 895, 1927, 2817, 3404
BsuRI GGCC 9 cut(s) 53, 143, 148, 275, 281, 938, 1398, 2281, 3325
BtgI CCRYGG 4 cut(s) 190, 1276, 2276, 3354
BtrI CACGTC 2 cut(s) 513, 3146
BtsI GCAGTG 5 cut(s) 785, 1356, 1430, 2050, 3157
BtsIMutI CAGTG 8 cut(s) 366, 408, 749, 785, 1356, 1430, 2050, 3157
BtuMI TCGCGA 1 cut(s) 120
BveI ACCTGC 2 cut(s) 2020, 2149
Cac8I GCNNGC 9 cut(s) 417, 421, 823, 1863, 2057, 2103, 2341, 2463, 3361
CaiI CAGNNNCTG 1 cut(s) 1994
CfoI GCGC 4 cut(s) 266, 1619, 1855, 3685
Cfr10I RCCGGY 3 cut(s) 281, 423, 2829
Cfr42I CCGCGG 1 cut(s) 193
Cfr9I CCCGGG 2 cut(s) 256, 276
CseI GACGC 8 cut(s) 24, 68, 225, 324, 335, 2224, 2740, 2822
CsiI ACCWGGT 3 cut(s) 537, 1132, 1453
DpnI GATC 9 cut(s) 897, 1205, 1320, 1929, 2049, 2432, 2819, 3406, 3649
DpnII GATC 9 cut(s) 895, 1203, 1318, 1927, 2047, 2430, 2817, 3404, 3647
DraI TTTAAA 1 cut(s) 3394
DriI GACNNNNNGTC 3 cut(s) 34, 226, 3568
EaeI YGGCCR 3 cut(s) 51, 936, 2279
Eam1104I CTCTTC 3 cut(s) 29, 1218, 1298
Eam1105I GACNNNNNGTC 3 cut(s) 34, 226, 3568
EarI CTCTTC 3 cut(s) 29, 1218, 1298
EciI GGCGGA 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 1468
Eco130I CCWWGG 3 cut(s) 1276, 2276, 3354
Eco24I GRGCYC 2 cut(s) 1470, 2939
Eco32I GATATC 1 cut(s) 2999
Eco47III AGCGCT 1 cut(s) 1854
Eco53kI GAGCTC 1 cut(s) 1468
Eco57I CTGAAG 6 cut(s) 1267, 1490, 1694, 1877, 2388, 3671
Eco72I CACGTG 1 cut(s) 2657
Eco88I CYCGRG 4 cut(s) 256, 276, 289, 3009
EcoICRI GAGCTC 1 cut(s) 1468
EcoNI CCTNNNNNAGG 1 cut(s) 1542
EcoO109I RGGNCCY 3 cut(s) 733, 793, 2765
EcoRI GAATTC 2 cut(s) 77, 3523
EcoRV GATATC 1 cut(s) 2999
EcoT14I CCWWGG 3 cut(s) 1276, 2276, 3354
EcoT22I ATGCAT 4 cut(s) 715, 958, 1477, 2178
EcoT38I GRGCYC 2 cut(s) 1470, 2939
ErhI CCWWGG 3 cut(s) 1276, 2276, 3354
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FauI CCCGC 3 cut(s) 296, 1854, 3352
FauNDI CATATG 1 cut(s) 2449
FbaI TGATCA 2 cut(s) 1203, 2047
FblI GTMKAC 2 cut(s) 1638, 1976
FriOI GRGCYC 2 cut(s) 1470, 2939
FspBI CTAG 8 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424, 3255
GlaI GCGC 4 cut(s) 265, 1618, 1854, 3684
GsaI CCCAGC 2 cut(s) 1926, 2100
GsuI CTGGAG 1 cut(s) 2877
HaeII RGCGCY 2 cut(s) 267, 1856
HaeIII GGCC 9 cut(s) 53, 143, 148, 275, 281, 938, 1398, 2281, 3325
HgaI GACGC 8 cut(s) 24, 68, 225, 324, 335, 2224, 2740, 2822
HhaI GCGC 4 cut(s) 266, 1619, 1855, 3685
Hin1I GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
Hin6I GCGC 4 cut(s) 264, 1617, 1853, 3683
HinP1I GCGC 4 cut(s) 264, 1617, 1853, 3683
HincII GTYRAC 4 cut(s) 325, 1212, 2259, 2730
HindII GTYRAC 4 cut(s) 325, 1212, 2259, 2730
HindIII AAGCTT 2 cut(s) 992, 1341
HpaI GTTAAC 1 cut(s) 1212
HphI GGTGA 8 cut(s) 527, 1039, 1148, 2057, 2230, 2449, 3395, 3601
Hpy99I CGWCG 5 cut(s) 40, 62, 241, 478, 1443
HpyCH4III ACNGT 3 cut(s) 617, 1555, 3716
HpyCH4IV ACGT 4 cut(s) 468, 512, 2656, 3145
HpyF10VI GCNNNNNNNGC 9 cut(s) 59, 192, 272, 429, 1178, 1340, 2093, 2102, 3020
HpySE526I ACGT 4 cut(s) 468, 512, 2656, 3145
Hsp92I GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
HspAI GCGC 4 cut(s) 264, 1617, 1853, 3683
Ksp22I TGATCA 2 cut(s) 1203, 2047
KspAI GTTAAC 1 cut(s) 1212
KspI CCGCGG 1 cut(s) 193
Kzo9I GATC 9 cut(s) 895, 1203, 1318, 1927, 2047, 2430, 2817, 3404, 3647
LweI GCATC 8 cut(s) 223, 611, 871, 943, 1156, 1484, 2185, 2611
MabI ACCWGGT 3 cut(s) 537, 1132, 1453
MaeI CTAG 8 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424, 3255
MaeII ACGT 4 cut(s) 468, 512, 2656, 3145
MaeIII GTNAC 6 cut(s) 464, 1555, 1791, 2236, 2392, 3475
MalI GATC 9 cut(s) 897, 1205, 1320, 1929, 2049, 2432, 2819, 3406, 3649
MboI GATC 9 cut(s) 895, 1203, 1318, 1927, 2047, 2430, 2817, 3404, 3647
MboII GAAGA 9 cut(s) 16, 560, 671, 869, 905, 1235, 1315, 2928, 3693
MfeI CAATTG 2 cut(s) 1569, 1799
MflI RGATCY 4 cut(s) 895, 1927, 2817, 3404
MlsI TGGCCA 1 cut(s) 2281
MluNI TGGCCA 1 cut(s) 2281
MlyI GAGTC 1 cut(s) 1982
MmeI TCCRAC 4 cut(s) 243, 2226, 3089, 3205
Mox20I TGGCCA 1 cut(s) 2281
Mph1103I ATGCAT 4 cut(s) 715, 958, 1477, 2178
MroXI GAANNNNTTC 1 cut(s) 3563
MscI TGGCCA 1 cut(s) 2281
MseI TTAA 9 cut(s) 701, 708, 789, 1071, 1211, 1733, 2405, 2622, 3393
MslI CAYNNNNRTG 1 cut(s) 3614
Msp20I TGGCCA 1 cut(s) 2281
MspA1I CMGCKG 3 cut(s) 192, 814, 1265
MspCI CTTAAG 2 cut(s) 700, 2404
MunI CAATTG 2 cut(s) 1569, 1799
Mva1269I GAATGC 3 cut(s) 2003, 2178, 3679
MvnI CGCG 3 cut(s) 120, 186, 192
MwoI GCNNNNNNNGC 9 cut(s) 59, 192, 272, 429, 1178, 1340, 2093, 2102, 3020
NciI CCSGG 7 cut(s) 110, 137, 257, 258, 277, 278, 446
NcoI CCATGG 3 cut(s) 1276, 2276, 3354
NdeI CATATG 1 cut(s) 2449
NdeII GATC 9 cut(s) 895, 1203, 1318, 1927, 2047, 2430, 2817, 3404, 3647
NheI GCTAGC 1 cut(s) 2339
NmuCI GTSAC 3 cut(s) 1555, 2236, 2392
NruI TCGCGA 1 cut(s) 120
NsiI ATGCAT 4 cut(s) 715, 958, 1477, 2178
NspI RCATGY 4 cut(s) 419, 1202, 1914, 3367
OliI CACNNNNGTG 1 cut(s) 3614
PaeI GCATGC 1 cut(s) 419
PasI CCCWGGG 1 cut(s) 88
PciI ACATGT 2 cut(s) 1198, 1910
PcsI WCGNNNNNNNCGW 1 cut(s) 446
PctI GAATGC 3 cut(s) 2003, 2178, 3679
PdmI GAANNNNTTC 1 cut(s) 3563
PfeI GAWTC 9 cut(s) 5, 127, 161, 532, 1025, 1120, 1525, 2543, 2616
PflMI CCANNNNNTGG 4 cut(s) 934, 1056, 2963, 3071
PfoI TCCNGGA 1 cut(s) 2854
PleI GAGTC 1 cut(s) 1981
PmaCI CACGTG 1 cut(s) 2657
PmlI CACGTG 1 cut(s) 2657
PpsI GAGTC 1 cut(s) 1981
Ppu21I YACGTR 1 cut(s) 2657
PpuMI RGGWCCY 3 cut(s) 733, 793, 2765
PscI ACATGT 2 cut(s) 1198, 1910
PshAI GACNNNNGTC 1 cut(s) 2832
PsiI TTATAA 3 cut(s) 2517, 3420, 3515
Psp124BI GAGCTC 1 cut(s) 1470
Psp5II RGGWCCY 3 cut(s) 733, 793, 2765
PspCI CACGTG 1 cut(s) 2657
PspFI CCCAGC 2 cut(s) 1922, 2096
PspPPI RGGWCCY 3 cut(s) 733, 793, 2765
PsrI GAACNNNNNNTAC 2 cut(s) 3084, 3116
PstI CTGCAG 1 cut(s) 1567
PstNI CAGNNNCTG 1 cut(s) 1994
PsuI RGATCY 4 cut(s) 895, 1927, 2817, 3404
PvuII CAGCTG 2 cut(s) 814, 1265
RruI TCGCGA 1 cut(s) 120
RseI CAYNNNNRTG 1 cut(s) 3614
SacI GAGCTC 1 cut(s) 1470
SacII CCGCGG 1 cut(s) 193
SaqAI TTAA 9 cut(s) 701, 708, 789, 1071, 1211, 1733, 2405, 2622, 3393
Sau3AI GATC 9 cut(s) 895, 1203, 1318, 1927, 2047, 2430, 2817, 3404, 3647
SchI GAGTC 1 cut(s) 1982
SexAI ACCWGGT 3 cut(s) 537, 1132, 1453
SfaNI GCATC 8 cut(s) 223, 611, 871, 943, 1156, 1484, 2185, 2611
SfcI CTRYAG 3 cut(s) 685, 1563, 1635
Sfr303I CCGCGG 1 cut(s) 193
SgrBI CCGCGG 1 cut(s) 193
SmaI CCCGGG 2 cut(s) 258, 278
SmiMI CAYNNNNRTG 1 cut(s) 3614
SmlI CTYRAG 5 cut(s) 602, 700, 899, 2404, 3528
SmoI CTYRAG 5 cut(s) 602, 700, 899, 2404, 3528
SphI GCATGC 1 cut(s) 419
SrfI GCCCGGGC 1 cut(s) 278
SspMI CTAG 8 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424, 3255
SstI GAGCTC 1 cut(s) 1470
StyI CCWWGG 3 cut(s) 1276, 2276, 3354
TaaI ACNGT 3 cut(s) 617, 1555, 3716
TaiI ACGT 4 cut(s) 471, 515, 2659, 3148
TaqI TCGA 8 cut(s) 27, 75, 102, 440, 1236, 1933, 2760, 3127
TatI WGTACW 2 cut(s) 1307, 1845
TauI GCSGC 5 cut(s) 195, 303, 435, 627, 1150
TfiI GAWTC 9 cut(s) 5, 127, 161, 532, 1025, 1120, 1525, 2543, 2616
Tru1I TTAA 9 cut(s) 701, 708, 789, 1071, 1211, 1733, 2405, 2622, 3393
Tru9I TTAA 9 cut(s) 701, 708, 789, 1071, 1211, 1733, 2405, 2622, 3393
TscAI CASTG 8 cut(s) 373, 415, 749, 785, 1356, 1437, 2057, 3157
TseFI GTSAC 3 cut(s) 1555, 2236, 2392
Tsp45I GTSAC 3 cut(s) 1555, 2236, 2392
TspGWI ACGGA 2 cut(s) 1412, 1943
TspMI CCCGGG 2 cut(s) 256, 276
TspRI CASTG 8 cut(s) 373, 415, 749, 785, 1356, 1437, 2057, 3157
Van91I CCANNNNNTGG 4 cut(s) 934, 1056, 2963, 3071
Vha464I CTTAAG 2 cut(s) 700, 2404
VneI GTGCAC 1 cut(s) 1449
XagI CCTNNNNNAGG 1 cut(s) 1542
XapI RAATTY 4 cut(s) 77, 1942, 2192, 3523
XceI RCATGY 4 cut(s) 419, 1202, 1914, 3367
XmaI CCCGGG 2 cut(s) 256, 276
XmiI GTMKAC 2 cut(s) 1638, 1976
XmnI GAANNNNTTC 1 cut(s) 3563
XspI CTAG 8 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424, 3255
Zsp2I ATGCAT 4 cut(s) 715, 958, 1477, 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.