FvH4_5g20920

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009459
Physical Location & Seq
Reverse (-)
12530990 .. 12542924
11935 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20920.t5

Sequence Viewer

Length: 3507 bp
ATGGAATCGCAGCCGAACAATCTCTTCGACACGGCGTCGCAGCCGGACACGGCCACCGACGCCTACACCTTCCTCGAATTCAACACCCAAGGCGAGGACTTCGACTACCCCGAGTTCCGCGACCCGATTCGGTCCCCGGTCGCCTGGCCCACGCCGTCCGATTCCCTATCCGACCCCGCCGACCGCGGAGGAGGAGGAGGAGGCGCGGGCTCCGACCACCAGTCGGACGCGTCGCCGGTGTCGGCGGCTCCTGGGAGCGCCACGAAGGCAAGGGCGGGTGGCTCCGGCAGTAGCGGCGGGAATAATAGTAGTAATAATCAGGTGGTGGATGCCCTAGCGTCTGGGATGAGTGTGTTGAACTTTGAGGACACTGGGGATGATGATAGCTATGAATTTGGGAAAGGGGATTTCACTGAGCATGCGTGCCGGTACTGCGGCGTCTCCAATCCGGCGTGTGTTGTGAGGTGTAATGTACCGTCGTGCCGCAAGTGGTTTTGTAATTCCAGGGGGAATACCTCCGGCTCTCATATTGTGAATCACCTGGTGCGTGCAAAACACAAGGAAGTCTGTCTTCATAAAGATAGTCCGTTAGGAGAAACAATCCTCGAGTGCTACAACTGTGGATGCCGCAATGTATTCCTTCTTGGATTTATTTCAGCCAAGACAGAGAGTGTTGTCGTTCTTCTTTGTAGGGAACCCTGCTTAAGTGTTAATGCATTGAAGGATATGAATTGGGACCTGAGCCAGTGGTGTCCCCTGATAGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGTCCCCTCTGAACAGGAGCAGCTGAGGGCACGCCAGATTAGTGCTCAACAAATAAACAAGGTAGAAGAACTCTGGAAGTCAAATCCTGATGCTTCCCTCGAAGATCTTGAGAAACCTGGTGTGGATGATGAACCCCAACCTGTGGTCATAAAGTATGAAGATGCATACCAGTATCAAAATGTGTTTGCACCACTTATCAAGCTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGCAAGGACAATCTCACCATTAGGTGGGATATCGGTCTTAACAAGAAGCGCGTTGCTTATTTTGTTTTTCCAAAGGAGGACAATGAGTTGCGTCTTGTACCTGGTGACGAGCTGCGGCTTCGTTATTCTGGGGATGCAGCTCATCCAGCATGGCAGTCTGTGGGACATGTGATCAAGTTAACTGCACAAGAGGAAGTTGCACTCGAACTTCGTGCTAGTCAGGGAGTTCCAGCCGATGTTAACCATGGCTTCAGTGTTGATTTTGTGTGGAAGAGTACAAGTTTTGATCGTATGCAGGGAGCCATGAAAGCTTTTGCAGTGGATGAGACAAGTGTTAGTGGGTATATTTACCATCACCTGTTAGGCCATGAAGTAGAGGTTCAAATGGTCCGTAATACACTACCTCGTCGTTTTGGTGCACCTGGTCTGCCAGAGCTCAATGCATCTCAGGTTTTTGCTGTGAAAAGTGTCCTCCAGAAGCCTATAAGTTTGATTCAAGGTCCACCTGGCACAGGAAAAACCGTCACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGGCAGGGACAGGTTTTGGTTTGTGCACCTAGTAATGTGGCTGTGGACCAACTAGCTGAAAAAATAAGTGCAACTGGGCTGAAGGTTGTTAGACTGTGTGCAAAATCAAGGGAAGCTGTCAGCTCACCGGTTGAGCATTTAACCCTTCACTATCAGGTTCGTCATCTTGACACATCAGAGAAGAGTGAACTTCACAAGTTACAGCAACTGAAGGATGAGCAAGGTGAATTGTCAAGTAGTGATGAGAAAAAGTACAAAGCGTTGAAACGGGCAACTGAGAGAGAGATTTCTCAGAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGAGATCCACGATTGGCGAATTTTAGATTCCGTCAGGTACTTATTGATGAGTCTACTCAGGCAACAGAGCCTGAATGCCTTATTCCTTTGGTACTCGGGGCAAAGCAGGTTGTTCTGGTTGGTGATCACTGCCAGCTTGGTCCAGTCATTATGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTTTTTGAACGCCTTGTTTCACTTGGTGTGAAACCAATTAGGTTGCAGGTCCAATACAGAATGCATCCAGCGCTGTCAGAGTTTCCTTCTAACAGCTTCTATGAGGGAACACTTCAAAATGGCGTCACCATCAATGAAAGGCAATCAACGGGAATTGATTTTCCATGGCCAGTTCCCAATCGTCCAATGTTCTTTTATGTTCAGATGGGTCAAGAGGAGATAAGTGCTAGTGGAACATCTTACCTGAACAGAACTGAAGCTGCTAATGTTGAGAAGATTGTCACAACATTCTTAAGGAGTGGTATCATCCCTAGTCAGATTGGGGTGATAACACCATATGAGGGACAGCGAGCATATATTGTGAACTATATGTCAAGAAATGGTGCTCTCAGACAGCAACTGTATAAGGAAATAGAGGTTGCAAGTGTAGATTCATTTCAGGGAAGGGAAAAAGATTATATCATCCTTTCATGTGTGAGGAGTAATGAGCATCAGGGAATTGGATTTCTTAACGATCCTCGGAGGCTCAATGTTGCCCTGACACGTGCTCGATATGGTATTGTTATTCTGGGGAATCCAAAAGTTCTAAGTAAACAGCCCTTGTGGAATAGCTTGTTGACACACTACAAGGAACATGAGTGCTTGGTTGAAGGTCCTCTAAATAACTTGAAGCAGAGTATGGTTCAATTTCCAAAGCCAAAAAAGATCTACAATGATCGTCGTCTGTTCGTTGGTGGTGGTCCTGGAGTTGTCCCAAGTGATAGTTATGGGTCTATTGCCCCATCCGGTCAGAGTGCTGATAGAAGAAGTGGTCGCGGTAGGGGTTCTTACCTACCTCCTGGTCCACCGAATGGTACCCACAAGCCTGGTGTACATCCTGCTGGATATCCAATGCCTAGGGCACCACTTGCACCATTTCATGGTGGTCCCCTTTCTCAACCCTATGCTATTCCAACTCGCGGAGCTGTGCATGGACCAGTAGGAGCTGTTCCTCATGTCCCTCAACCTGGAAGCCGAGGGTTTGGAGCTGGACGTGGCAATGCTGGTACTCCTATTGGCAGTCATCTCCCACACCAGCAAGGCACACAGCAGAATGTAGGAAATCTGGGATCCAATTTCAACTTTCCTGCTCTGGAAAATCCTAATAGTCAGCCATCTGTGGGCGGTCCATTATCTCAACCTGGATTTGTCAATAATATGCCAGGTCAAGGGCCGAGCCAAGCATTTCGCGATGGATTTTCTATGGCGGGCATGTCTCAGGAGTTCTTGGGTGATGACTTTAAAAGTCAGGGATCACATGTTCCTTATAATGTTGCTGACTTCTCCACTCAGGATGTTCTTGTCTGTCAGATTTCTGTAAAGCATTTTGGGGCAGTTCATAATTTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

1169

Amino Acids

127.65

Weight (kDa)

6.56

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_11 PF13086 109 - 229 1.3e-23 AAA domain
AAA_30 PF13604 109 - 310 3.1e-14 AAA domain
ResIII PF04851 109 - 179 2.9e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 116 - 310 2e-11 AAA domain
AAA_11 PF13086 211 - 312 6.3e-29 AAA domain
AAA_12 PF13087 321 - 517 7.4e-62 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3423
Acc36I ACCTGC 2 cut(s) 2023, 2152
Acc65I GGTACC 1 cut(s) 2969
AccB1I GGYRCC 2 cut(s) 2969, 3016
AccB7I CCANNNNNTGG 4 cut(s) 937, 1059, 2966, 3035
AccI GTMKAC 1 cut(s) 1979
AccII CGCG 8 cut(s) 120, 186, 206, 230, 1086, 2931, 3075, 3345
AclWI GGATC 5 cut(s) 1925, 2624, 3219, 3232, 3415
AcoI YGGCCR 2 cut(s) 51, 2282
AcsI RAATTY 3 cut(s) 77, 392, 1945
AcuI CTGAAG 4 cut(s) 1270, 1697, 1826, 2391
AcvI CACGTG 1 cut(s) 2660
AcyI GRCGYC 4 cut(s) 35, 60, 438, 2238
AdeI CACNNNGTG 2 cut(s) 544, 2141
AfeI AGCGCT 1 cut(s) 2187
AflII CTTAAG 2 cut(s) 703, 2407
AflIII ACRYGT 5 cut(s) 228, 1201, 1913, 2657, 3412
AgeI ACCGGT 1 cut(s) 1723
AhdI GACNNNNNGTC 2 cut(s) 34, 220
AjiI CACGTC 1 cut(s) 3149
AloI GAACNNNNNNTCC 2 cut(s) 3399, 3431
Alw21I GWGCWC 6 cut(s) 841, 1456, 1473, 1624, 2503, 2665
Alw26I GTCTC 4 cut(s) 445, 1355, 2118, 3375
Alw44I GTGCAC 2 cut(s) 1452, 1620
AlwI GGATC 5 cut(s) 1925, 2624, 3219, 3232, 3415
AlwNI CAGNNNCTG 3 cut(s) 1804, 1997, 2515
Ama87I CYCGRG 3 cut(s) 110, 605, 2021
Aor51HI AGCGCT 1 cut(s) 2187
AoxI GGCC 5 cut(s) 51, 146, 1399, 2282, 3326
ApaLI GTGCAC 2 cut(s) 1452, 1620
ApeKI GCWGC 8 cut(s) 10, 40, 814, 1147, 1172, 1568, 2090, 2375
ApoI RAATTY 3 cut(s) 77, 392, 1945
AsiGI ACCGGT 1 cut(s) 1723
Asp718I GGTACC 1 cut(s) 2969
AspA2I CCTAGG 1 cut(s) 3011
AspLEI GCGC 4 cut(s) 206, 260, 1086, 2188
AsuC2I CCSGG 1 cut(s) 137
AvaI CYCGRG 3 cut(s) 110, 605, 2021
AvrII CCTAGG 1 cut(s) 3011
BaeGI GKGCMC 4 cut(s) 826, 1456, 1624, 3019
BalI TGGCCA 1 cut(s) 2284
BamHI GGATCC 1 cut(s) 3224
BanI GGYRCC 2 cut(s) 2969, 3016
BanII GRGCYC 2 cut(s) 212, 1473
BauI CACGAG 1 cut(s) 2094
BbrPI CACGTG 1 cut(s) 2660
BbsI GAAGAC 1 cut(s) 563
Bbv12I GWGCWC 6 cut(s) 841, 1456, 1473, 1624, 2503, 2665
BbvCI CCTCAGC 1 cut(s) 818
BbvI GCAGC 8 cut(s) 22, 52, 826, 1134, 1184, 1580, 2102, 2362
BccI CCATC 6 cut(s) 1395, 2252, 2314, 2905, 3277, 3341
BceAI ACGGC 3 cut(s) 48, 66, 139
BcgI CGANNNNNNTGC 2 cut(s) 1229, 1263
BclI TGATCA 2 cut(s) 1206, 2050
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 4 cut(s) 445, 1355, 2118, 3375
BfaI CTAG 7 cut(s) 335, 1251, 1626, 1649, 2343, 2427, 3012
BfmI CTRYAG 1 cut(s) 1566
BfoI RGCGCY 2 cut(s) 261, 2189
BfrI CTTAAG 2 cut(s) 703, 2407
BfuAI ACCTGC 2 cut(s) 2023, 2152
BglI GCCNNNNNGGC 1 cut(s) 266
BglII AGATCT 2 cut(s) 898, 2820
BlnI CCTAGG 1 cut(s) 3011
BmeRI GACNNNNNGTC 2 cut(s) 34, 220
BmeT110I CYCGRG 3 cut(s) 110, 605, 2021
BmgBI CACGTC 1 cut(s) 3149
BmrI ACTGGG 3 cut(s) 381, 1680, 2104
BmsI GCATC 8 cut(s) 319, 614, 874, 946, 1159, 1487, 2188, 2614
BmuI ACTGGG 3 cut(s) 381, 1680, 2104
BpiI GAAGAC 1 cut(s) 563
BpmI CTGGAG 3 cut(s) 1493, 1947, 2880
Bpu10I CCTNAGC 2 cut(s) 740, 818
BpuEI CTTGAG 1 cut(s) 923
BpuMI CCSGG 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 2660
BsaHI GRCGYC 4 cut(s) 35, 60, 438, 2238
BsaWI WCCGGW 2 cut(s) 1723, 2900
Bse118I RCCGGY 3 cut(s) 235, 426, 1723
Bse1I ACTGG 9 cut(s) 220, 376, 745, 964, 1675, 2069, 2110, 2285, 3092
Bse3DI GCAATG 2 cut(s) 637, 3160
BseMI GCAATG 2 cut(s) 637, 3160
BseNI ACTGG 9 cut(s) 220, 376, 745, 964, 1675, 2069, 2110, 2285, 3092
BseRI GAGGAG 6 cut(s) 204, 207, 210, 213, 2345, 2608
BseSI GKGCMC 4 cut(s) 826, 1456, 1624, 3019
BseXI GCAGC 8 cut(s) 22, 52, 826, 1134, 1184, 1580, 2102, 2362
BseYI CCCAGC 1 cut(s) 2099
BsgI GTGCAG 1 cut(s) 1203
Bsh1236I CGCG 8 cut(s) 120, 186, 206, 230, 1086, 2931, 3075, 3345
Bsh1285I CGRYCG 2 cut(s) 141, 184
BshFI GGCC 5 cut(s) 53, 148, 1401, 2284, 3328
BshNI GGYRCC 2 cut(s) 2969, 3016
BshTI ACCGGT 1 cut(s) 1723
BsiEI CGRYCG 2 cut(s) 141, 184
BsiHKAI GWGCWC 6 cut(s) 841, 1456, 1473, 1624, 2503, 2665
BsiHKCI CYCGRG 3 cut(s) 110, 605, 2021
BsiSI CCGG 9 cut(s) 44, 137, 236, 285, 427, 449, 519, 1724, 2901
BsmAI GTCTC 4 cut(s) 445, 1355, 2118, 3375
BsmBI CGTCTC 1 cut(s) 445
BsmI GAATGC 2 cut(s) 2006, 2181
BsnI GGCC 5 cut(s) 53, 148, 1401, 2284, 3328
BsoBI CYCGRG 3 cut(s) 110, 605, 2021
Bsp1286I GDGCHC 9 cut(s) 212, 826, 841, 1456, 1473, 1624, 2503, 2665, 3019
Bsp1407I TGTACA 1 cut(s) 2986
Bsp19I CCATGG 2 cut(s) 1279, 2279
Bsp68I TCGCGA 1 cut(s) 3345
BspANI GGCC 5 cut(s) 53, 148, 1401, 2284, 3328
BspFNI CGCG 8 cut(s) 120, 186, 206, 230, 1086, 2931, 3075, 3345
BspMAI CTGCAG 1 cut(s) 1570
BspMI ACCTGC 2 cut(s) 2023, 2152
BspPI GGATC 5 cut(s) 1925, 2624, 3219, 3232, 3415
BspT107I GGYRCC 2 cut(s) 2969, 3016
BspTI CTTAAG 2 cut(s) 703, 2407
BsrDI GCAATG 2 cut(s) 637, 3160
BsrFI RCCGGY 3 cut(s) 235, 426, 1723
BsrGI TGTACA 1 cut(s) 2986
BsrI ACTGG 9 cut(s) 220, 376, 745, 964, 1675, 2069, 2110, 2285, 3092
BssAI RCCGGY 3 cut(s) 235, 426, 1723
BssNI GRCGYC 4 cut(s) 35, 60, 438, 2238
BssSI CACGAG 1 cut(s) 2094
BssT1I CCWWGG 4 cut(s) 88, 1279, 2279, 3011
Bst2BI CACGAG 1 cut(s) 2094
Bst4CI ACNGT 5 cut(s) 477, 620, 1558, 1692, 2517
Bst6I CTCTTC 3 cut(s) 29, 1301, 1772
BstACI GRCGYC 4 cut(s) 35, 60, 438, 2238
BstAFI CTTAAG 2 cut(s) 703, 2407
BstAPI GCANNNNNTGC 2 cut(s) 2096, 3023
BstAUI TGTACA 1 cut(s) 2986
BstBAI YACGTR 1 cut(s) 2660
BstC8I GCNNGC 9 cut(s) 208, 420, 424, 549, 826, 2060, 2106, 2466, 3364
BstDSI CCRYGG 3 cut(s) 184, 1279, 2279
BstENI CCTNNNNNAGG 1 cut(s) 1545
BstFNI CGCG 8 cut(s) 120, 186, 206, 230, 1086, 2931, 3075, 3345
BstH2I RGCGCY 2 cut(s) 261, 2189
BstHHI GCGC 4 cut(s) 206, 260, 1086, 2188
BstMAI GTCTC 4 cut(s) 445, 1355, 2118, 3375
BstMCI CGRYCG 2 cut(s) 141, 184
BstNSI RCATGY 5 cut(s) 422, 1205, 1917, 3370, 3416
BstSFI CTRYAG 1 cut(s) 1566
BstSLI GKGCMC 4 cut(s) 826, 1456, 1624, 3019
BstUI CGCG 8 cut(s) 120, 186, 206, 230, 1086, 2931, 3075, 3345
BstV1I GCAGC 8 cut(s) 22, 52, 826, 1134, 1184, 1580, 2102, 2362
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 4 cut(s) 898, 1930, 2820, 3224
BstXI CCANNNNNNTGG 1 cut(s) 2981
BstYI RGATCY 4 cut(s) 898, 1930, 2820, 3224
BsuRI GGCC 5 cut(s) 53, 148, 1401, 2284, 3328
BtgI CCRYGG 3 cut(s) 184, 1279, 2279
BtgZI GCGATG 1 cut(s) 3360
BtrI CACGTC 1 cut(s) 3149
BtsI GCAGTG 3 cut(s) 788, 1359, 2053
BtsIMutI CAGTG 7 cut(s) 369, 411, 752, 788, 1294, 1359, 2053
BtuMI TCGCGA 1 cut(s) 3345
BveI ACCTGC 2 cut(s) 2023, 2152
Cac8I GCNNGC 9 cut(s) 208, 420, 424, 549, 826, 2060, 2106, 2466, 3364
CaiI CAGNNNCTG 3 cut(s) 1804, 1997, 2515
CfoI GCGC 4 cut(s) 206, 260, 1086, 2188
Cfr10I RCCGGY 3 cut(s) 235, 426, 1723
Cfr42I CCGCGG 1 cut(s) 187
CseI GACGC 8 cut(s) 24, 68, 219, 236, 327, 427, 1115, 2227
CsiI ACCWGGT 4 cut(s) 540, 910, 1135, 1456
CspAI ACCGGT 1 cut(s) 1723
DraI TTTAAA 1 cut(s) 3397
DraIII CACNNNGTG 2 cut(s) 544, 2141
DriI GACNNNNNGTC 2 cut(s) 34, 220
EaeI YGGCCR 2 cut(s) 51, 2282
Eam1104I CTCTTC 3 cut(s) 29, 1301, 1772
Eam1105I GACNNNNNGTC 2 cut(s) 34, 220
EarI CTCTTC 3 cut(s) 29, 1301, 1772
Ecl136II GAGCTC 1 cut(s) 1471
Eco130I CCWWGG 4 cut(s) 88, 1279, 2279, 3011
Eco24I GRGCYC 2 cut(s) 212, 1473
Eco32I GATATC 2 cut(s) 1066, 3002
Eco47III AGCGCT 1 cut(s) 2187
Eco53kI GAGCTC 1 cut(s) 1471
Eco57I CTGAAG 4 cut(s) 1270, 1697, 1826, 2391
Eco72I CACGTG 1 cut(s) 2660
Eco88I CYCGRG 3 cut(s) 110, 605, 2021
EcoICRI GAGCTC 1 cut(s) 1471
EcoNI CCTNNNNNAGG 1 cut(s) 1545
EcoO109I RGGNCCY 3 cut(s) 736, 796, 2768
EcoRI GAATTC 1 cut(s) 77
EcoRV GATATC 2 cut(s) 1066, 3002
EcoT14I CCWWGG 4 cut(s) 88, 1279, 2279, 3011
EcoT22I ATGCAT 4 cut(s) 718, 961, 1480, 2181
EcoT38I GRGCYC 2 cut(s) 212, 1473
ErhI CCWWGG 4 cut(s) 88, 1279, 2279, 3011
Esp3I CGTCTC 1 cut(s) 445
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FauI CCCGC 5 cut(s) 184, 199, 268, 290, 3355
FauNDI CATATG 1 cut(s) 2452
FbaI TGATCA 2 cut(s) 1206, 2050
FblI GTMKAC 1 cut(s) 1979
FriOI GRGCYC 2 cut(s) 212, 1473
FspBI CTAG 7 cut(s) 335, 1251, 1626, 1649, 2343, 2427, 3012
GlaI GCGC 4 cut(s) 205, 259, 1085, 2187
GsaI CCCAGC 1 cut(s) 2103
GsuI CTGGAG 3 cut(s) 1493, 1947, 2880
HaeII RGCGCY 2 cut(s) 261, 2189
HaeIII GGCC 5 cut(s) 53, 148, 1401, 2284, 3328
HapII CCGG 9 cut(s) 44, 137, 236, 285, 427, 449, 519, 1724, 2901
HgaI GACGC 8 cut(s) 24, 68, 219, 236, 327, 427, 1115, 2227
HhaI GCGC 4 cut(s) 206, 260, 1086, 2188
Hin1I GRCGYC 4 cut(s) 35, 60, 438, 2238
Hin6I GCGC 4 cut(s) 204, 258, 1084, 2186
HinP1I GCGC 4 cut(s) 204, 258, 1084, 2186
HincII GTYRAC 3 cut(s) 1215, 1276, 2733
HindII GTYRAC 3 cut(s) 1215, 1276, 2733
HindIII AAGCTT 2 cut(s) 995, 1344
HpaI GTTAAC 2 cut(s) 1215, 1276
HpaII CCGG 9 cut(s) 44, 137, 236, 285, 427, 449, 519, 1724, 2901
Hpy99I CGWCG 6 cut(s) 40, 62, 235, 481, 1446, 2838
HpyCH4III ACNGT 5 cut(s) 477, 620, 1558, 1692, 2517
HpyCH4IV ACGT 2 cut(s) 2659, 3148
HpySE526I ACGT 2 cut(s) 2659, 3148
Hsp92I GRCGYC 4 cut(s) 35, 60, 438, 2238
HspAI GCGC 4 cut(s) 204, 258, 1084, 2186
KpnI GGTACC 1 cut(s) 2973
Ksp22I TGATCA 2 cut(s) 1206, 2050
KspAI GTTAAC 2 cut(s) 1215, 1276
KspI CCGCGG 1 cut(s) 187
LmnI GCTCC 9 cut(s) 215, 253, 255, 287, 811, 1334, 3077, 3098, 3140
Lsp1109I GCAGC 8 cut(s) 22, 52, 826, 1134, 1184, 1580, 2102, 2362
LweI GCATC 8 cut(s) 319, 614, 874, 946, 1159, 1487, 2188, 2614
MabI ACCWGGT 4 cut(s) 540, 910, 1135, 1456
MaeI CTAG 7 cut(s) 335, 1251, 1626, 1649, 2343, 2427, 3012
MaeII ACGT 2 cut(s) 2659, 3148
MaeIII GTNAC 5 cut(s) 1139, 1558, 1794, 2239, 2395
MfeI CAATTG 1 cut(s) 1572
MflI RGATCY 4 cut(s) 898, 1930, 2820, 3224
MhlI GDGCHC 9 cut(s) 212, 826, 841, 1456, 1473, 1624, 2503, 2665, 3019
MlsI TGGCCA 1 cut(s) 2284
MluI ACGCGT 1 cut(s) 228
MluNI TGGCCA 1 cut(s) 2284
MlyI GAGTC 1 cut(s) 1985
MmeI TCCRAC 4 cut(s) 195, 204, 237, 3092
Mox20I TGGCCA 1 cut(s) 2284
Mph1103I ATGCAT 4 cut(s) 718, 961, 1480, 2181
MscI TGGCCA 1 cut(s) 2284
Msp20I TGGCCA 1 cut(s) 2284
MspA1I CMGCKG 2 cut(s) 186, 817
MspCI CTTAAG 2 cut(s) 703, 2407
MspI CCGG 9 cut(s) 44, 137, 236, 285, 427, 449, 519, 1724, 2901
MunI CAATTG 1 cut(s) 1572
Mva1269I GAATGC 2 cut(s) 2006, 2181
MvnI CGCG 8 cut(s) 120, 186, 206, 230, 1086, 2931, 3075, 3345
NciI CCSGG 1 cut(s) 137
NcoI CCATGG 2 cut(s) 1279, 2279
NdeI CATATG 1 cut(s) 2452
NmeAIII GCCGAG 2 cut(s) 3155, 3354
NmuCI GTSAC 4 cut(s) 1139, 1558, 2239, 2395
NruI TCGCGA 1 cut(s) 3345
NsiI ATGCAT 4 cut(s) 718, 961, 1480, 2181
NspI RCATGY 5 cut(s) 422, 1205, 1917, 3370, 3416
PaeI GCATGC 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 605
PciI ACATGT 3 cut(s) 1201, 1913, 3412
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 2 cut(s) 2006, 2181
PfeI GAWTC 9 cut(s) 5, 127, 161, 535, 1028, 1528, 1953, 2546, 2689
PflMI CCANNNNNTGG 4 cut(s) 937, 1059, 2966, 3035
PfoI TCCNGGA 1 cut(s) 2857
PinAI ACCGGT 1 cut(s) 1723
PleI GAGTC 1 cut(s) 1984
PmaCI CACGTG 1 cut(s) 2660
PmlI CACGTG 1 cut(s) 2660
PpsI GAGTC 1 cut(s) 1984
Ppu21I YACGTR 1 cut(s) 2660
PpuMI RGGWCCY 3 cut(s) 736, 796, 2768
PscI ACATGT 3 cut(s) 1201, 1913, 3412
PsiI TTATAA 1 cut(s) 3423
Psp124BI GAGCTC 1 cut(s) 1473
Psp5II RGGWCCY 3 cut(s) 736, 796, 2768
PspCI CACGTG 1 cut(s) 2660
PspFI CCCAGC 1 cut(s) 2099
PspPPI RGGWCCY 3 cut(s) 736, 796, 2768
PspXI VCTCGAGB 1 cut(s) 605
PsrI GAACNNNNNNTAC 2 cut(s) 3087, 3119
PstI CTGCAG 1 cut(s) 1570
PstNI CAGNNNCTG 3 cut(s) 1804, 1997, 2515
PsuI RGATCY 4 cut(s) 898, 1930, 2820, 3224
PvuII CAGCTG 1 cut(s) 817
RruI TCGCGA 1 cut(s) 3345
SacI GAGCTC 1 cut(s) 1473
SacII CCGCGG 1 cut(s) 187
SchI GAGTC 1 cut(s) 1985
SduI GDGCHC 9 cut(s) 212, 826, 841, 1456, 1473, 1624, 2503, 2665, 3019
SexAI ACCWGGT 4 cut(s) 540, 910, 1135, 1456
SfaNI GCATC 8 cut(s) 319, 614, 874, 946, 1159, 1487, 2188, 2614
SfcI CTRYAG 1 cut(s) 1566
Sfr274I CTCGAG 1 cut(s) 605
Sfr303I CCGCGG 1 cut(s) 187
SgrAI CRCCGGYG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 187
SlaI CTCGAG 1 cut(s) 605
SmlI CTYRAG 4 cut(s) 605, 703, 902, 2407
SmoI CTYRAG 4 cut(s) 605, 703, 902, 2407
SphI GCATGC 1 cut(s) 422
SspMI CTAG 7 cut(s) 335, 1251, 1626, 1649, 2343, 2427, 3012
SstI GAGCTC 1 cut(s) 1473
StyI CCWWGG 4 cut(s) 88, 1279, 2279, 3011
TaaI ACNGT 5 cut(s) 477, 620, 1558, 1692, 2517
TaiI ACGT 2 cut(s) 2662, 3151
TaqI TCGA 7 cut(s) 27, 75, 102, 606, 894, 1239, 2665
TaqII GACCGA 2 cut(s) 120, 1058
TatI WGTACW 3 cut(s) 1310, 1848, 2986
TauI GCSGC 6 cut(s) 248, 297, 438, 486, 630, 1153
TfiI GAWTC 9 cut(s) 5, 127, 161, 535, 1028, 1528, 1953, 2546, 2689
TscAI CASTG 7 cut(s) 376, 418, 752, 788, 1294, 1359, 2060
TseFI GTSAC 4 cut(s) 1139, 1558, 2239, 2395
TseI GCWGC 8 cut(s) 10, 40, 814, 1147, 1172, 1568, 2090, 2375
Tsp45I GTSAC 4 cut(s) 1139, 1558, 2239, 2395
TspGWI ACGGA 3 cut(s) 576, 1415, 1946
TspRI CASTG 7 cut(s) 376, 418, 752, 788, 1294, 1359, 2060
Van91I CCANNNNNTGG 4 cut(s) 937, 1059, 2966, 3035
Vha464I CTTAAG 2 cut(s) 703, 2407
VneI GTGCAC 2 cut(s) 1452, 1620
XagI CCTNNNNNAGG 1 cut(s) 1545
XapI RAATTY 3 cut(s) 77, 392, 1945
XceI RCATGY 5 cut(s) 422, 1205, 1917, 3370, 3416
XhoI CTCGAG 1 cut(s) 605
XmaJI CCTAGG 1 cut(s) 3011
XmiI GTMKAC 1 cut(s) 1979
XspI CTAG 7 cut(s) 335, 1251, 1626, 1649, 2343, 2427, 3012
Zsp2I ATGCAT 4 cut(s) 718, 961, 1480, 2181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.