Rroxscaffold_3G00250970

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009459
Physical Location & Seq
Forward (+)
44829471 .. 44841405
11935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250970.1

Sequence Viewer

Length: 3837 bp
ATGGAATCGCAGCCGAACAACCTCTTCGACACGGCGTCGCAGCCGGACACGGCCACCGACGCCTACACTTTCCTCGAATTCAACACCCAGGGCGAGGACTTCGACTACCCGGAGTTTCGCGACCCGATTCGCTCCCCGGTGGCCTGGCCCACGCCGTCCGATTCCTTATCGGACCCTGCCGACCGCGACCGAGACCGCGGCGGAGGAGGTGGTGGTGGTGGCTCCGACCACCAGTCTGATGCGTCGCCTGTTTCGGCTGCCCCGGGCAGCGCCACGAAGGCCCGGGCCGGTGGCTCGGGAAGTAGCGGCGGGAATAATAATCAGGTGGTTGACGCCCTAGCGTCTGGGATGAGTGTGTTGAACTTTGAGGACACTGGGGACGATGATAGCTATGAGTTTGGAAAGGGGGATTTCACTGAGCATGCGTGCCGGTACTGCGGCGTGTCGAACCCGGCGTGCGTTGTGAGGTGTAACGTGCCGTCGTGCCGGAAGTGGTTTTGTAATTCCAGGGGGAACACGTCCGGCTCTCATATTGTGAATCACCTGGTTCGTGCAAAACACAAGGAAGTCTGTCTTCATAAAGATAGCCCATTAGGAGAAACAATCCTTGAGTGCTACAACTGTGGATGCCGCAATGTATTCCTTCTTGGATTTATTTCAGCCAAGACAGAGAGTGTTGTTGTTCTTCTCTGTAGGGAACCCTGCTTAAGTGTTAATGCATTGAAGGATATGAACTGGGACCTGAGCCAGTGGTGTCCCCTGATTGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGTCCCCTCCGAACAGGAGCAGCTGAGGGCACGCCAGATTAGTGCTCAACAAATAAACAAGGTAGAAGAACTCTGGAAGTCAAATCCTGATGCTTCCCTTGAAGATCTTGAGAAGCCTGGTGTGGATGATGAACCCCAACCTGTGGCCGTAAAGTATGAGGATGCGTACCAGTATCAAAATGTGTTTGCACCACTTATCAAGCTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGCAAGGACAATCTCACCATTAGGTGGGACATTGGTCTTAACAAGAAGCGTGTCGCTTATTTTATTTTTCCAAAGGAGGACAATGAGATTCGTCTTGTACCTGGTGATGAGCTGCGGCTTCGTTATTCTGGGGATGCAGCTCATCCAGCATGGCAGTCTGTGGGACATGTGATCAAGTTAACTGCACAAGAGGAGGTTGCGCTCGAACTTCGTGCTAGTCAGGGAGTTCCAGCTGATGTGAACCATGGCTTCAGCGTTGATTTCGTGTGGAAGAGTACAAGTTTTGATCGTATGCAGGGAGCCATGAAAGCTTTTGCAGTGGATGAGACAAGTGTTAGTGGGTATATTTACCATCACTTGTTAGGCCATGAAGTAGAGGTTCAGATGGTCCGTAATACACTGCCTCGTCGTTTTGGTGCACCTGGTCTGCCAGAGCTCAATGCATCTCAGGTTTTTGCTGTGAAAAGTGTTCTTCAGAAGCCTATAAGTTTGATTCAAGGTCCACCTGGCACAGGAAAAACTGTCACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGGCAGGGACAGGTCTTGGTTTGCGCTCCTAGTAATGTGGCTGTAGACCAACTAGCTGAAAAAATAAGTGCAACTGGGCTGAAGGTTGTTAGACTTTGTGCAAAATCAAGGGAAGCTGTCAGCTCTCCTGTTGAGCATTTAACCCTTCACTATCAGGTTCGTCATCTTGACACATCAGAAAAGAGTGAACTTCACAAGTTACAGCAATTGAAGGATGAGCAAGGCGAATTGTCAAGTAGTGATGAGAAGAAGTACAAAGCGCTGAAGCGGGCAACTGAGAGGGAGATTTCTCAGAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGAGATCCTCGATTGGCAAATTTTAGGTTCCGTCAGGTACTTATTGATGAGTCTACTCAGGCAACAGAGCCTGAATGCCTTATTCCTTTGGTACTAGGGGCAAAGCAGGTTGTTTTGGTTGGTGATCACTGCCAGCTTGGTCCAGTCATTATGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTTTTTGAACGCCTCGTTTTACTTGGTGTGAAACCAATTAGGTTGCAGGTCCAATACAGAATGCATCCAGCCTTGTCAGAATTTCCTTCCAACAGCTTCTATGAGGGAACACTCCAAAATGGCGTCACAATCAATGAAAGGCAATCAACGGGAATTGATTTTCCATGGCCAGTTCCCAATCGTCCCATGTTCTTTTATGTTCAGATGGGGCAAGAGGAGATAAGTGCTAGCGGAACATCTTACCTGAACAGAACTGAAGCTGCTAATGTTGAAAAGATTGTCACAACATTCTTAAGGAGTGGTGTCGTACCTAGTCAGATCGGGGTGATAACACCATATGAGGGACAGCGAGCATATATTGTGAACTATATGTCAAGAAATGGTGCTCTCAGGCAGCAACTTTATAAGGAAATAGAGGTCGCAAGTGTAGATTCATTTCAAGGAAGGGAAAAAGATTATATCATCCTTTCATGTGTGAGGAGTAATGAGCATCAGGGCATTGGATTCCTTAATGACCCTCGGAGGCTCAATGTTGCCCTAACACGTGCTCGGTATGGTATTGTTATTCTGGGGAACCCTAAAGTTCTAAGTAAACAGCCCTTGTGGAATAGCTTGTTGACGCACTACAAGGAACATGAGTGCTTGGTCGAAGGTCCTCTAAATAACTTGAAGCAGAGTATGGTTCAATTTCCAAAGCCCAAAAAGATCTACAATGATCGGCGTCTGTTCTTTGGTGGTGGTCCTGGAGTTGTCCCAAGTGATAGTTATGGGTCTATTCCCCCATCCGGTCAGAGTGCTGATAGAAGAAGTGGTCGTGGTAGGGGCTCTTACCTACCTCCTGGTCCACCGAATGGTACCCACAAGCCTGGGGTACATCCTGCTGGATATCCAATGCCTCGGGCACCACTTGCACCATTTCATGGTGGTCCCCTTTCTCAACCCTATGCTATTCCAACTCGTGGAGCCGTGCATGGACCAGTAGGAGCTGTTCCTCATGTCCCTCAACCTGGAAGTCGAGGTTTTGGAGCTGGACGTGGCAGTGCTGGTGCTCCTATTGGCAGTCATCTCCAACACCAGCAAGGCACACAGCAGAATGTAGGAAATCTGGGGTCTAACTTTAACTTTCCTGCTCTGGAAAATCCTAATAGTCAGCCATCTGTGGGCGGTCCATTATCTCAACCTGGATTTGTCAATAATATGCCAGTTCAAGGGCCAAGCCAAACATTTCGTGATGGATTTTCCATGGCGGGCATGTCTCAGGAGTTCTTGGGTGATGACTTTAAAAGTCAGGGATCTCATGTTCCTTATAACGTCGCTGACTTTTCCACTCAGGCCTCTCAAAGTGGATATGCTGTTGATTATGTTACACAAGGAGCGCAAGGAGGATTTCCAGGCAATTTTATAAACCAGAATTCTCAAGCAGGCTACTCTCGATTTGGTACAGGGAATGACTTCATGTCTCAGGACTACATGGCTCATGGTACACAAGGTCTATTTACCCAGGTGGGCTACAATGACCCATCACAGGATGATGGATCGCAGAACCACTATGGAGTAGGGAATGCTAATGCGCTTCAGTCTCAGGGATATATGAATTCTCTGTACTCCCAACCTTTTACCCACTACAACACACAGCCAATGAACTTGCAGGCCCCACAACAGCAGGCGCCTCAGCAGGGTCAGAGCTCCCAAAATCAGAAGATTCACTACAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

1278

Amino Acids

139.73

Weight (kDa)

6.35

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 141 - 294 9e-73 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 346 - 435 1.8e-26 RNA helicase UPF1, 1B domain
AAA_11 PF13086 491 - 611 2.1e-23 AAA domain
AAA_30 PF13604 491 - 692 5.7e-14 AAA domain
ResIII PF04851 491 - 561 4.9e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 498 - 692 3.5e-11 AAA domain
AAA_11 PF13086 593 - 694 1e-28 AAA domain
AAA_12 PF13087 703 - 899 1.2e-61 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2523, 3426, 3521
Acc36I ACCTGC 2 cut(s) 2026, 2155
Acc65I GGTACC 1 cut(s) 2972
AccB1I GGYRCC 3 cut(s) 2972, 3019, 3784
AccB7I CCANNNNNTGG 5 cut(s) 940, 1062, 2969, 3038, 3077
AccI GTMKAC 2 cut(s) 1644, 1982
AccII CGCG 3 cut(s) 120, 186, 198
AclWI GGATC 3 cut(s) 1928, 3418, 3661
AcoI YGGCCR 3 cut(s) 51, 942, 2285
AcsI RAATTY 5 cut(s) 77, 1948, 2198, 3529, 3712
AcuI CTGAAG 6 cut(s) 1273, 1496, 1700, 1883, 2394, 3677
AcvI CACGTG 1 cut(s) 2663
AcyI GRCGYC 6 cut(s) 35, 60, 333, 2241, 2839, 3785
AfeI AGCGCT 1 cut(s) 1860
AflII CTTAAG 2 cut(s) 706, 2410
AflIII ACRYGT 4 cut(s) 516, 1204, 1916, 2660
AhdI GACNNNNNGTC 3 cut(s) 34, 232, 3574
AjiI CACGTC 2 cut(s) 519, 3152
AloI GAACNNNNNNTCC 2 cut(s) 3402, 3434
Alw21I GWGCWC 7 cut(s) 844, 1459, 1476, 2506, 2668, 3169, 3806
Alw26I GTCTC 6 cut(s) 186, 1358, 2121, 3378, 3582, 3702
Alw44I GTGCAC 1 cut(s) 1455
AlwI GGATC 3 cut(s) 1928, 3418, 3661
AlwNI CAGNNNCTG 1 cut(s) 2000
Ama87I CYCGRG 4 cut(s) 262, 282, 295, 3015
Aor51HI AGCGCT 1 cut(s) 1860
ApaLI GTGCAC 1 cut(s) 1455
ApoI RAATTY 5 cut(s) 77, 1948, 2198, 3529, 3712
Asp700I GAANNNNTTC 1 cut(s) 3569
Asp718I GGTACC 1 cut(s) 2972
AspLEI GCGC 7 cut(s) 272, 1240, 1625, 1861, 3496, 3691, 3787
AsuC2I CCSGG 7 cut(s) 110, 137, 263, 264, 283, 284, 452
AsuHPI GGTGA 6 cut(s) 533, 1045, 1154, 2063, 2455, 3401
AsuNHI GCTAGC 1 cut(s) 2345
AvaI CYCGRG 4 cut(s) 262, 282, 295, 3015
BaeGI GKGCMC 3 cut(s) 829, 1459, 3022
BalI TGGCCA 1 cut(s) 2287
BanI GGYRCC 3 cut(s) 2972, 3019, 3784
BanII GRGCYC 3 cut(s) 1476, 2945, 3806
BarI GAAGNNNNNNTAC 1 cut(s) 3809
BauI CACGAG 2 cut(s) 2097, 3075
BbrPI CACGTG 1 cut(s) 2663
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 7 cut(s) 844, 1459, 1476, 2506, 2668, 3169, 3806
BbvCI CCTCAGC 2 cut(s) 821, 3789
BccI CCATC 8 cut(s) 1398, 1417, 2317, 2908, 3280, 3344, 3644, 3646
BceAI ACGGC 6 cut(s) 48, 66, 139, 463, 929, 3068
BcgI CGANNNNNNTGC 2 cut(s) 1232, 1266
BclI TGATCA 2 cut(s) 1209, 2053
BcnI CCSGG 7 cut(s) 110, 137, 263, 264, 283, 284, 452
BcoDI GTCTC 6 cut(s) 186, 1358, 2121, 3378, 3582, 3702
BfaI CTAG 7 cut(s) 338, 1254, 1629, 1652, 2024, 2346, 2430
BfmI CTRYAG 3 cut(s) 691, 1569, 1641
BfoI RGCGCY 3 cut(s) 273, 1862, 3788
BfrI CTTAAG 2 cut(s) 706, 2410
BfuAI ACCTGC 2 cut(s) 2026, 2155
BglI GCCNNNNNGGC 1 cut(s) 278
BglII AGATCT 2 cut(s) 901, 2823
BmeRI GACNNNNNGTC 3 cut(s) 34, 232, 3574
BmeT110I CYCGRG 4 cut(s) 262, 282, 295, 3015
BmgBI CACGTC 2 cut(s) 519, 3152
BmrI ACTGGG 4 cut(s) 384, 745, 1683, 2107
BmsI GCATC 8 cut(s) 229, 617, 877, 949, 1162, 1490, 2191, 2617
BmtI GCTAGC 1 cut(s) 2349
BmuI ACTGGG 4 cut(s) 384, 745, 1683, 2107
BoxI GACNNNNGTC 1 cut(s) 1071
BpiI GAAGAC 1 cut(s) 566
BpmI CTGGAG 2 cut(s) 1950, 2883
Bpu10I CCTNAGC 3 cut(s) 743, 821, 3789
BpuEI CTTGAG 3 cut(s) 629, 926, 3519
BpuMI CCSGG 7 cut(s) 110, 137, 263, 264, 283, 284, 452
BsaAI YACGTR 1 cut(s) 2663
BsaHI GRCGYC 6 cut(s) 35, 60, 333, 2241, 2839, 3785
BsaI GGTCTC 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 2903
Bse118I RCCGGY 2 cut(s) 287, 429
Bse3DI GCAATG 1 cut(s) 640
BseMI GCAATG 1 cut(s) 640
BseRI GAGGAG 4 cut(s) 219, 1244, 2348, 2611
BseSI GKGCMC 3 cut(s) 829, 1459, 3022
BseYI CCCAGC 1 cut(s) 2102
BsgI GTGCAG 1 cut(s) 1206
Bsh1236I CGCG 3 cut(s) 120, 186, 198
Bsh1285I CGRYCG 2 cut(s) 184, 190
BshNI GGYRCC 3 cut(s) 2972, 3019, 3784
BsiEI CGRYCG 2 cut(s) 184, 190
BsiHKAI GWGCWC 7 cut(s) 844, 1459, 1476, 2506, 2668, 3169, 3806
BsiHKCI CYCGRG 4 cut(s) 262, 282, 295, 3015
BsmAI GTCTC 6 cut(s) 186, 1358, 2121, 3378, 3582, 3702
BsmI GAATGC 3 cut(s) 2009, 2184, 3685
Bso31I GGTCTC 1 cut(s) 186
BsoBI CYCGRG 4 cut(s) 262, 282, 295, 3015
Bsp19I CCATGG 3 cut(s) 1282, 2282, 3360
Bsp68I TCGCGA 1 cut(s) 120
BspFNI CGCG 3 cut(s) 120, 186, 198
BspMAI CTGCAG 1 cut(s) 1573
BspMI ACCTGC 2 cut(s) 2026, 2155
BspOI GCTAGC 1 cut(s) 2349
BspPI GGATC 3 cut(s) 1928, 3418, 3661
BspT107I GGYRCC 3 cut(s) 2972, 3019, 3784
BspTI CTTAAG 2 cut(s) 706, 2410
BspTNI GGTCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 640
BsrFI RCCGGY 2 cut(s) 287, 429
BssAI RCCGGY 2 cut(s) 287, 429
BssNI GRCGYC 6 cut(s) 35, 60, 333, 2241, 2839, 3785
BssSI CACGAG 2 cut(s) 2097, 3075
BssT1I CCWWGG 3 cut(s) 1282, 2282, 3360
Bst2BI CACGAG 2 cut(s) 2097, 3075
Bst4CI ACNGT 2 cut(s) 623, 1561
Bst6I CTCTTC 2 cut(s) 29, 1304
BstACI GRCGYC 6 cut(s) 35, 60, 333, 2241, 2839, 3785
BstAFI CTTAAG 2 cut(s) 706, 2410
BstAPI GCANNNNNTGC 2 cut(s) 2099, 3026
BstBAI YACGTR 1 cut(s) 2663
BstDSI CCRYGG 4 cut(s) 196, 1282, 2282, 3360
BstENI CCTNNNNNAGG 1 cut(s) 1548
BstFNI CGCG 3 cut(s) 120, 186, 198
BstH2I RGCGCY 3 cut(s) 273, 1862, 3788
BstHHI GCGC 7 cut(s) 272, 1240, 1625, 1861, 3496, 3691, 3787
BstMAI GTCTC 6 cut(s) 186, 1358, 2121, 3378, 3582, 3702
BstMCI CGRYCG 2 cut(s) 184, 190
BstMWI GCNNNNNNNGC 8 cut(s) 59, 278, 435, 1184, 1346, 2099, 2108, 3026
BstNSI RCATGY 4 cut(s) 425, 1208, 1920, 3373
BstPAI GACNNNNGTC 1 cut(s) 1071
BstSFI CTRYAG 3 cut(s) 691, 1569, 1641
BstSLI GKGCMC 3 cut(s) 829, 1459, 3022
BstUI CGCG 3 cut(s) 120, 186, 198
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 4 cut(s) 901, 1933, 2823, 3410
BstXI CCANNNNNNTGG 1 cut(s) 2984
BstYI RGATCY 4 cut(s) 901, 1933, 2823, 3410
BtgI CCRYGG 4 cut(s) 196, 1282, 2282, 3360
BtrI CACGTC 2 cut(s) 519, 3152
BtsI GCAGTG 5 cut(s) 791, 1362, 1436, 2056, 3163
BtsIMutI CAGTG 8 cut(s) 372, 414, 755, 791, 1362, 1436, 2056, 3163
BtuMI TCGCGA 1 cut(s) 120
BveI ACCTGC 2 cut(s) 2026, 2155
CaiI CAGNNNCTG 1 cut(s) 2000
CfoI GCGC 7 cut(s) 272, 1240, 1625, 1861, 3496, 3691, 3787
Cfr10I RCCGGY 2 cut(s) 287, 429
Cfr42I CCGCGG 1 cut(s) 199
Cfr9I CCCGGG 2 cut(s) 262, 282
CseI GACGC 8 cut(s) 24, 68, 231, 330, 341, 2230, 2746, 2828
CsiI ACCWGGT 3 cut(s) 543, 1138, 1459
DinI GGCGCC 1 cut(s) 3786
DraI TTTAAA 1 cut(s) 3400
DriI GACNNNNNGTC 3 cut(s) 34, 232, 3574
EaeI YGGCCR 3 cut(s) 51, 942, 2285
Eam1104I CTCTTC 2 cut(s) 29, 1304
Eam1105I GACNNNNNGTC 3 cut(s) 34, 232, 3574
EarI CTCTTC 2 cut(s) 29, 1304
EciI GGCGGA 1 cut(s) 216
Ecl136II GAGCTC 2 cut(s) 1474, 3804
Eco130I CCWWGG 3 cut(s) 1282, 2282, 3360
Eco147I AGGCCT 1 cut(s) 3452
Eco24I GRGCYC 3 cut(s) 1476, 2945, 3806
Eco31I GGTCTC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 3005
Eco47III AGCGCT 1 cut(s) 1860
Eco53kI GAGCTC 2 cut(s) 1474, 3804
Eco57I CTGAAG 6 cut(s) 1273, 1496, 1700, 1883, 2394, 3677
Eco72I CACGTG 1 cut(s) 2663
Eco88I CYCGRG 4 cut(s) 262, 282, 295, 3015
EcoICRI GAGCTC 2 cut(s) 1474, 3804
EcoNI CCTNNNNNAGG 1 cut(s) 1548
EcoO109I RGGNCCY 4 cut(s) 739, 799, 2771, 3769
EcoRI GAATTC 3 cut(s) 77, 3529, 3712
EcoRV GATATC 1 cut(s) 3005
EcoT14I CCWWGG 3 cut(s) 1282, 2282, 3360
EcoT22I ATGCAT 3 cut(s) 721, 1483, 2184
EcoT38I GRGCYC 3 cut(s) 1476, 2945, 3806
EgeI GGCGCC 1 cut(s) 3786
EheI GGCGCC 1 cut(s) 3786
ErhI CCWWGG 3 cut(s) 1282, 2282, 3360
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FauI CCCGC 3 cut(s) 302, 1860, 3358
FauNDI CATATG 1 cut(s) 2455
FbaI TGATCA 2 cut(s) 1209, 2053
FblI GTMKAC 2 cut(s) 1644, 1982
FriOI GRGCYC 3 cut(s) 1476, 2945, 3806
FspBI CTAG 7 cut(s) 338, 1254, 1629, 1652, 2024, 2346, 2430
GlaI GCGC 7 cut(s) 271, 1239, 1624, 1860, 3495, 3690, 3786
GsaI CCCAGC 1 cut(s) 2106
GsuI CTGGAG 2 cut(s) 1950, 2883
HaeII RGCGCY 3 cut(s) 273, 1862, 3788
HgaI GACGC 8 cut(s) 24, 68, 231, 330, 341, 2230, 2746, 2828
HhaI GCGC 7 cut(s) 272, 1240, 1625, 1861, 3496, 3691, 3787
Hin1I GRCGYC 6 cut(s) 35, 60, 333, 2241, 2839, 3785
Hin6I GCGC 7 cut(s) 270, 1238, 1623, 1859, 3494, 3689, 3785
HinP1I GCGC 7 cut(s) 270, 1238, 1623, 1859, 3494, 3689, 3785
HincII GTYRAC 3 cut(s) 331, 1218, 2736
HindII GTYRAC 3 cut(s) 331, 1218, 2736
HindIII AAGCTT 2 cut(s) 998, 1347
HpaI GTTAAC 1 cut(s) 1218
HphI GGTGA 6 cut(s) 533, 1045, 1154, 2063, 2455, 3401
Hpy99I CGWCG 6 cut(s) 40, 62, 247, 484, 1449, 3434
HpyAV CCTTC 9 cut(s) 271, 653, 718, 1675, 1754, 1804, 2214, 2556, 2762
HpyCH4III ACNGT 2 cut(s) 623, 1561
HpyCH4IV ACGT 5 cut(s) 474, 518, 2662, 3151, 3429
HpyF10VI GCNNNNNNNGC 8 cut(s) 59, 278, 435, 1184, 1346, 2099, 2108, 3026
HpySE526I ACGT 5 cut(s) 474, 518, 2662, 3151, 3429
Hsp92I GRCGYC 6 cut(s) 35, 60, 333, 2241, 2839, 3785
HspAI GCGC 7 cut(s) 270, 1238, 1623, 1859, 3494, 3689, 3785
KasI GGCGCC 1 cut(s) 3784
KpnI GGTACC 1 cut(s) 2976
Ksp22I TGATCA 2 cut(s) 1209, 2053
KspAI GTTAAC 1 cut(s) 1218
KspI CCGCGG 1 cut(s) 199
LweI GCATC 8 cut(s) 229, 617, 877, 949, 1162, 1490, 2191, 2617
MabI ACCWGGT 3 cut(s) 543, 1138, 1459
MaeI CTAG 7 cut(s) 338, 1254, 1629, 1652, 2024, 2346, 2430
MaeII ACGT 5 cut(s) 474, 518, 2662, 3151, 3429
MaeIII GTNAC 6 cut(s) 470, 1561, 1797, 2242, 2398, 3481
MfeI CAATTG 2 cut(s) 1575, 1805
MflI RGATCY 4 cut(s) 901, 1933, 2823, 3410
MlsI TGGCCA 1 cut(s) 2287
MluNI TGGCCA 1 cut(s) 2287
Mly113I GGCGCC 1 cut(s) 3785
MlyI GAGTC 1 cut(s) 1988
MmeI TCCRAC 4 cut(s) 249, 2232, 3095, 3211
Mox20I TGGCCA 1 cut(s) 2287
Mph1103I ATGCAT 3 cut(s) 721, 1483, 2184
MroXI GAANNNNTTC 1 cut(s) 3569
MscI TGGCCA 1 cut(s) 2287
MslI CAYNNNNRTG 1 cut(s) 3828
Msp20I TGGCCA 1 cut(s) 2287
MspA1I CMGCKG 3 cut(s) 198, 820, 1271
MspCI CTTAAG 2 cut(s) 706, 2410
MunI CAATTG 2 cut(s) 1575, 1805
Mva1269I GAATGC 3 cut(s) 2009, 2184, 3685
MvnI CGCG 3 cut(s) 120, 186, 198
MwoI GCNNNNNNNGC 8 cut(s) 59, 278, 435, 1184, 1346, 2099, 2108, 3026
NarI GGCGCC 1 cut(s) 3785
NciI CCSGG 7 cut(s) 110, 137, 263, 264, 283, 284, 452
NcoI CCATGG 3 cut(s) 1282, 2282, 3360
NdeI CATATG 1 cut(s) 2455
NheI GCTAGC 1 cut(s) 2345
NmuCI GTSAC 3 cut(s) 1561, 2242, 2398
NruI TCGCGA 1 cut(s) 120
NsiI ATGCAT 3 cut(s) 721, 1483, 2184
NspI RCATGY 4 cut(s) 425, 1208, 1920, 3373
PaeI GCATGC 1 cut(s) 425
PasI CCCWGGG 1 cut(s) 88
PceI AGGCCT 1 cut(s) 3452
PciI ACATGT 2 cut(s) 1204, 1916
PcsI WCGNNNNNNNCGW 1 cut(s) 452
PctI GAATGC 3 cut(s) 2009, 2184, 3685
PdmI GAANNNNTTC 1 cut(s) 3569
PflMI CCANNNNNTGG 5 cut(s) 940, 1062, 2969, 3038, 3077
PfoI TCCNGGA 1 cut(s) 2860
PleI GAGTC 1 cut(s) 1987
PluTI GGCGCC 1 cut(s) 3788
PmaCI CACGTG 1 cut(s) 2663
PmlI CACGTG 1 cut(s) 2663
PpsI GAGTC 1 cut(s) 1987
Ppu21I YACGTR 1 cut(s) 2663
PpuMI RGGWCCY 3 cut(s) 739, 799, 2771
PscI ACATGT 2 cut(s) 1204, 1916
PshAI GACNNNNGTC 1 cut(s) 1071
PsiI TTATAA 3 cut(s) 2523, 3426, 3521
Psp124BI GAGCTC 2 cut(s) 1476, 3806
Psp5II RGGWCCY 3 cut(s) 739, 799, 2771
PspCI CACGTG 1 cut(s) 2663
PspFI CCCAGC 1 cut(s) 2102
PspPPI RGGWCCY 3 cut(s) 739, 799, 2771
PsrI GAACNNNNNNTAC 2 cut(s) 3090, 3122
PstI CTGCAG 1 cut(s) 1573
PstNI CAGNNNCTG 1 cut(s) 2000
PsuI RGATCY 4 cut(s) 901, 1933, 2823, 3410
PvuII CAGCTG 2 cut(s) 820, 1271
RruI TCGCGA 1 cut(s) 120
RseI CAYNNNNRTG 1 cut(s) 3828
SacI GAGCTC 2 cut(s) 1476, 3806
SacII CCGCGG 1 cut(s) 199
SchI GAGTC 1 cut(s) 1988
SexAI ACCWGGT 3 cut(s) 543, 1138, 1459
SfaNI GCATC 8 cut(s) 229, 617, 877, 949, 1162, 1490, 2191, 2617
SfcI CTRYAG 3 cut(s) 691, 1569, 1641
SfoI GGCGCC 1 cut(s) 3786
Sfr303I CCGCGG 1 cut(s) 199
SgrBI CCGCGG 1 cut(s) 199
SmaI CCCGGG 2 cut(s) 264, 284
SmiMI CAYNNNNRTG 1 cut(s) 3828
SmlI CTYRAG 5 cut(s) 608, 706, 905, 2410, 3534
SmoI CTYRAG 5 cut(s) 608, 706, 905, 2410, 3534
SphI GCATGC 1 cut(s) 425
SrfI GCCCGGGC 1 cut(s) 284
SseBI AGGCCT 1 cut(s) 3452
SspDI GGCGCC 1 cut(s) 3784
SspMI CTAG 7 cut(s) 338, 1254, 1629, 1652, 2024, 2346, 2430
SstI GAGCTC 2 cut(s) 1476, 3806
StuI AGGCCT 1 cut(s) 3452
StyI CCWWGG 3 cut(s) 1282, 2282, 3360
TaaI ACNGT 2 cut(s) 623, 1561
TaiI ACGT 5 cut(s) 477, 521, 2665, 3154, 3432
TaqI TCGA 9 cut(s) 27, 75, 102, 446, 1242, 1939, 2766, 3133, 3550
TaqII GACCGA 1 cut(s) 204
TatI WGTACW 3 cut(s) 1313, 1851, 3720
TauI GCSGC 5 cut(s) 201, 309, 441, 633, 1156
TscAI CASTG 8 cut(s) 379, 421, 755, 791, 1362, 1443, 2063, 3163
TseFI GTSAC 3 cut(s) 1561, 2242, 2398
Tsp45I GTSAC 3 cut(s) 1561, 2242, 2398
TspGWI ACGGA 2 cut(s) 1418, 1949
TspMI CCCGGG 2 cut(s) 262, 282
TspRI CASTG 8 cut(s) 379, 421, 755, 791, 1362, 1443, 2063, 3163
Van91I CCANNNNNTGG 5 cut(s) 940, 1062, 2969, 3038, 3077
Vha464I CTTAAG 2 cut(s) 706, 2410
VneI GTGCAC 1 cut(s) 1455
XagI CCTNNNNNAGG 1 cut(s) 1548
XapI RAATTY 5 cut(s) 77, 1948, 2198, 3529, 3712
XceI RCATGY 4 cut(s) 425, 1208, 1920, 3373
XmaI CCCGGG 2 cut(s) 262, 282
XmiI GTMKAC 2 cut(s) 1644, 1982
XmnI GAANNNNTTC 1 cut(s) 3569
XspI CTAG 7 cut(s) 338, 1254, 1629, 1652, 2024, 2346, 2430
Zsp2I ATGCAT 3 cut(s) 721, 1483, 2184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.