MD04G1136100.v1.1

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009459
Physical Location & Seq
Reverse (-)
22318865 .. 22328069
9205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1136100.v1.1.491

Sequence Viewer

Length: 2982 bp
ATGGTCCGAGCCAAACACAAGGAAGTCTGTCTTCATAAAGATAGCCCATTGGGAGAAACAATCCTTGAATGCTACAACTGTGGATGTCGTAATGTATTCCTTCTGGGATTTATTTCAGCCAAGACAGAGAGTGTTGTTGTTCTTCTTTGTAGGGAACCCTGCTTAAGTGTTAATGCATTGAAGGATATGAACTGGGATCTGAGCCAGTGGTGTCCCCTGATAGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGTCCCGTCTGAACAGGAGCAGCTAAGGGCGCGCCAAATCAGTGCTCAACAAATAAATAAAGTAGAAGAACTTTGGAAGACCAATCCTGATGCTTCCCTTGAAGATCTCGAGAAGCCGGGTGTGGACGATGAACCCCAACCTGTGGCCTTGAAGTATGAAGATGCTTACCAGTATCAAAATGTGTTTGCACCACTTATTAAACTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGTAAAGATAATGTCACAATTCGGTGGGATATTGGTCTTAATAAGAAGCGTATTGCTTTATTTGTGTTTACAAAGGAAGACAATGAATTGCGCCTTGTACCTGGTGATGAGTTGCGGCTTCGTTATTCTGGAGATGCAGCTCATCCAGCATGGCAGTCAGTGGGACATGTTATCAAGTTAACTCCACAAGAAGAGGTTGCACTTGAACTTCGTGCCAGTCAGGGTGTTCCAGTTGATCTGAACCATGGCTTCAGTGTTGACTTTGTCTGGAAGAGTACAAGTTTTGATCGGATGCAGGGAGCTATGAAAGCTTTTGCAGTGGACGAGACAAGTGTTAGTGGATATATTTATCATCATTTGTTAGGCCATGAAGTAGAGGTTCAAATGGTCCGTAACACATTGCCTCGCCGTTTTGGTGCACCTGGTCTTCCGGAGCTCAATGCATCTCAAGTTTTTGCTGTAAAGAGTGTCCTTCAGAAGCCTATAAGTTTGATCCAGGGTCCACCTGGCACAGGAAAAACTGTGACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGCCAGGGACAGGTTTTGGTGTGTGCTCCTAGTAATGTTGCTGTAGACCAACTAGCTGAGAAGATAAGTGCAACTGGGTTGAAGGTTGTTAGACTTTGTGCAAAATCAAGGGAAGCCGTAAGTTCTCCTGTTGAGCATTTAACCCTTCATTACCAGGTTCGTCATCTTGACATGTCAGAAAAGAGTGAACTTCACAAGCTGCAACAATTGAAGGATGAGCAAGGAGAGTTGTCTAGTAATGATGAGAAAAAATATAAGGCACTGAAGCGGGCAACCGAGAGGGAGATTTCTCAGAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGAGATCCTCGATTGGCCAATTTTAGGTTCCGTCAGGTTGAAGTACTTATTGATGAGTCGACTCAGGCTACAGAACCTGAATGCCTGATTCCTTTGGTTCTTGGGGCAAAGCAGGTTGTTCTTGTTGGAGATCACTGCCAGCTTGGTCCAGTCATTATGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTTTTTGAACGCCTCGTTATACTTGGTGTGAAACCAATTAGATTGCAGGTAATTGGTCCACAGTTTTCAGTGTCTGCATGGGTAGCTGCGCATAGTATCATTGCTAGTGTTCAATACCGAATGCATCCAGCCTTGTCAGAATTTCCATCTAACAGCTTCTACGAGGGCACACTTCAAAATGGAGTTACCATCAATGAAAGGCAATCATCGGGCATTGATTTTCCTTGGCCGGTTCCCAATCGTCCCATGTTCTTTTATGTACAGATGGGGCAAGAGGAGATAAGTGCTAGTGGAACATCCTATCTGAACAGAACTGAGGCTGCTAACGTTGAAAAGATTGTCACAACATTCTTGAGGAGTGGCGTAGTCCCTAGTCAGATTGGAGTGATCACACCATATGAGGGACAGAGAGCTTATATTGTGAACCATATGTCGAGAAATGGTGCTCTCAGACAGCAACTTTATAAGGAAATTGAGGTTGCCAGCGTAGATTCATTTCAAGGACGGGAAAAGGATTATATCATCCTTTCGTGTGTAAGGAGTAATGAGCATCAGGGCATCGGATTCCTTAATGATCCTCGCAGGCTCAATGTTGCTCTAACACGCGCTCGATATGGTATTGTCATTCTTGGAAACCCTAAAGTTCTTAGTAAGCAGCCACTGTGGAATAGCCTGTTGACTCATTACAAGATCTACAATGATCGTCGCCTATTCTATGGTGGTGGTCCTGGAGTTATCCCCAATGACAGTTATGGGTCTGTTGCCTCAGCTGGCCAAAGTGCTGATAGAAGAAGTAATCGGGGTAGGGGTATGTTCTTACCTACCTCCTGGCCCACCAATGGTACCCAGCCTGGTGTACATCCGGCTGGATATCCATTACCTCGGGCACCTCTTTCGCCATTTCATGGAGGACCCCTTTCTCAACCTTATGCTATTCCAACTCGTGGAGCTGTGCATGGACCAGTTGGAGCTGTTCCTCATGTCCCTCAACCTGGAAGTCGTGGTTTTGGGGCAGGACGTGGGAATGCCGGTTCTCCAATTGGCAGTCATCTTCCGCACCAGCAAGGCACACAACAAAATGTTGGGAATATGGGGTCTACCTTCAACATTCCTTCTCTTGAAAATCCAAATAGCCAACCATCTGTTGGTGGCCCCTTATCTCAACATGGATTTGTCAATAATATGCCAGTTCAAGGGCCAAGCCAAACGTTTCGTGATGGATTTTCCATGGCGGGCATGTCTCAGGAATGTTTGGGTGATGACTTTAAAAGTCAGGGATCACATGTTCCGTATAATGTTGCTGACTTCTCCACTCAGGCTTCTCAAAGTGGATATGGTGTTGATTATGTTACTCAAGGAGCACAAGGTGGGTTTCCAGGGAACTTTCTGAGGAATACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

994

Amino Acids

109.17

Weight (kDa)

8.0

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 1 - 113 4.7e-50 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 165 - 254 2.1e-26 RNA helicase UPF1, 1B domain
AAA_11 PF13086 310 - 431 1.1e-23 AAA domain
AAA_30 PF13604 310 - 513 6.9e-14 AAA domain
ResIII PF04851 310 - 380 3.8e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 317 - 513 1.8e-11 AAA domain
AAA_11 PF13086 413 - 515 1.3e-26 AAA domain
AAA_12 PF13087 527 - 740 2.3e-57 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2046
Acc16I TGCGCA 1 cut(s) 1671
Acc36I ACCTGC 2 cut(s) 1489, 1618
Acc65I GGTACC 1 cut(s) 2423
AccB1I GGYRCC 2 cut(s) 2423, 2467
AccB7I CCANNNNNTGG 4 cut(s) 397, 2486, 2525, 2726
AccI GTMKAC 3 cut(s) 1101, 1445, 2678
AccII CGCG 2 cut(s) 286, 2187
AccIII TCCGGA 1 cut(s) 925
AciI CCGC 4 cut(s) 610, 1324, 2636, 2813
AclI AACGTT 2 cut(s) 1908, 2789
AclWI GGATC 5 cut(s) 204, 982, 1385, 2150, 2866
AcoI YGGCCR 3 cut(s) 1401, 1808, 2353
AcsI RAATTY 1 cut(s) 1721
AcuI CTGAAG 3 cut(s) 730, 953, 1340
AdeI CACNNNGTG 1 cut(s) 2948
AfaI GTAC 6 cut(s) 594, 772, 1431, 1842, 2425, 2439
AflII CTTAAG 1 cut(s) 163
AflIII ACRYGT 4 cut(s) 661, 1227, 1373, 2863
AjiI CACGTC 1 cut(s) 2600
AjuI GAANNNNNNNTTGG 2 cut(s) 1397, 1429
AloI GAACNNNNNNTCC 4 cut(s) 2596, 2628, 2850, 2882
Alw21I GWGCWC 6 cut(s) 301, 916, 933, 1084, 2029, 2944
Alw26I GTCTC 3 cut(s) 815, 1584, 2826
Alw44I GTGCAC 1 cut(s) 912
AlwI GGATC 5 cut(s) 204, 982, 1385, 2150, 2866
AlwNI CAGNNNCTG 3 cut(s) 1463, 1655, 2242
Ama87I CYCGRG 2 cut(s) 362, 2463
Aor13HI TCCGGA 1 cut(s) 925
AoxI GGCC 9 cut(s) 399, 859, 1057, 1401, 1808, 2353, 2411, 2731, 2777
ApaLI GTGCAC 1 cut(s) 912
ApeKI GCWGC 8 cut(s) 274, 632, 1028, 1255, 1556, 1667, 1901, 2236
ApoI RAATTY 1 cut(s) 1721
AscI GGCGCGCC 1 cut(s) 284
Asp718I GGTACC 1 cut(s) 2423
AspLEI GCGC 5 cut(s) 286, 288, 588, 1672, 2189
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 2 cut(s) 611, 2849
AvaI CYCGRG 2 cut(s) 362, 2463
AvaII GGWCC 9 cut(s) 4, 256, 883, 995, 1532, 1637, 2306, 2492, 2540
BaeGI GKGCMC 3 cut(s) 916, 1751, 2470
BalI TGGCCA 2 cut(s) 1403, 2355
BanI GGYRCC 2 cut(s) 2423, 2467
BanII GRGCYC 1 cut(s) 933
BauI CACGAG 2 cut(s) 1560, 2523
BbsI GAAGAC 4 cut(s) 23, 338, 579, 914
Bbv12I GWGCWC 6 cut(s) 301, 916, 933, 1084, 2029, 2944
BbvCI CCTCAGC 1 cut(s) 2347
BbvI GCAGC 8 cut(s) 286, 644, 1040, 1242, 1568, 1654, 1888, 2248
BccI CCATC 5 cut(s) 1735, 1778, 1840, 2728, 2792
BceAI ACGGC 2 cut(s) 888, 1157
BcgI CGANNNNNNTGC 2 cut(s) 2457, 2491
BclI TGATCA 1 cut(s) 1968
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 3 cut(s) 815, 1584, 2826
BfaI CTAG 7 cut(s) 1086, 1109, 1290, 1686, 1869, 1953, 2980
BfmI CTRYAG 3 cut(s) 1026, 1098, 1455
BfrI CTTAAG 1 cut(s) 163
BfuAI ACCTGC 2 cut(s) 1489, 1618
BglII AGATCT 2 cut(s) 358, 2271
BisI GCNGC 9 cut(s) 275, 611, 633, 1029, 1256, 1557, 1668, 1902, 2237
BlsI GCNGC 9 cut(s) 276, 612, 634, 1030, 1257, 1558, 1669, 1903, 2238
BmcAI AGTACT 1 cut(s) 1431
Bme18I GGWCC 9 cut(s) 4, 256, 883, 995, 1532, 1637, 2306, 2492, 2540
BmeT110I CYCGRG 2 cut(s) 362, 2463
BmgBI CACGTC 1 cut(s) 2600
BmiI GGNNCC 9 cut(s) 156, 258, 996, 1415, 1815, 2425, 2469, 2494, 2734
BmrI ACTGGG 3 cut(s) 202, 1140, 1570
BmsI GCATC 8 cut(s) 334, 406, 619, 777, 947, 1714, 2140, 2148
BmuI ACTGGG 3 cut(s) 202, 1140, 1570
BpiI GAAGAC 4 cut(s) 23, 338, 579, 914
BpmI CTGGAG 3 cut(s) 645, 1407, 2331
Bpu10I CCTNAGC 2 cut(s) 278, 2347
BpuEI CTTGAG 3 cut(s) 927, 1954, 2919
BpuMI CCSGG 1 cut(s) 372
BsaJI CCNNGG 7 cut(s) 739, 991, 1060, 1805, 2462, 2808, 2957
BsaWI WCCGGW 1 cut(s) 925
Bse118I RCCGGY 2 cut(s) 1810, 2609
Bse3DI GCAATG 2 cut(s) 893, 1680
BseAI TCCGGA 1 cut(s) 925
BseDI CCNNGG 7 cut(s) 739, 991, 1060, 1805, 2462, 2808, 2957
BseGI GGATG 8 cut(s) 89, 637, 792, 1276, 1705, 1877, 2103, 2440
BseMI GCAATG 2 cut(s) 893, 1680
BsePI GCGCGC 1 cut(s) 284
BseRI GAGGAG 2 cut(s) 1871, 1951
BseSI GKGCMC 3 cut(s) 916, 1751, 2470
BseXI GCAGC 8 cut(s) 286, 644, 1040, 1242, 1568, 1654, 1888, 2248
BseYI CCCAGC 2 cut(s) 1565, 2427
Bsh1236I CGCG 2 cut(s) 286, 2187
BshFI GGCC 9 cut(s) 401, 861, 1059, 1403, 1810, 2355, 2413, 2733, 2779
BshNI GGYRCC 2 cut(s) 2423, 2467
BsiHKAI GWGCWC 6 cut(s) 301, 916, 933, 1084, 2029, 2944
BsiHKCI CYCGRG 2 cut(s) 362, 2463
BsiSI CCGG 5 cut(s) 371, 926, 1811, 2444, 2610
BslFI GGGAC 8 cut(s) 198, 242, 672, 1077, 1809, 1934, 1998, 2549
BsmAI GTCTC 3 cut(s) 815, 1584, 2826
BsmFI GGGAC 8 cut(s) 198, 242, 672, 1077, 1809, 1934, 1998, 2549
BsmI GAATGC 4 cut(s) 74, 1472, 1707, 2611
BsnI GGCC 9 cut(s) 401, 861, 1059, 1403, 1810, 2355, 2413, 2733, 2779
BsoBI CYCGRG 2 cut(s) 362, 2463
Bsp1286I GDGCHC 8 cut(s) 301, 916, 933, 1084, 1751, 2029, 2470, 2944
Bsp13I TCCGGA 1 cut(s) 925
Bsp1407I TGTACA 2 cut(s) 1840, 2437
Bsp19I CCATGG 2 cut(s) 739, 2808
BspACI CCGC 4 cut(s) 610, 1324, 2636, 2813
BspANI GGCC 9 cut(s) 401, 861, 1059, 1403, 1810, 2355, 2413, 2733, 2779
BspEI TCCGGA 1 cut(s) 925
BspFNI CGCG 2 cut(s) 286, 2187
BspLI GGNNCC 9 cut(s) 156, 258, 996, 1415, 1815, 2425, 2469, 2494, 2734
BspMAI CTGCAG 1 cut(s) 1030
BspMI ACCTGC 2 cut(s) 1489, 1618
BspPI GGATC 5 cut(s) 204, 982, 1385, 2150, 2866
BspT107I GGYRCC 2 cut(s) 2423, 2467
BspTI CTTAAG 1 cut(s) 163
BsrDI GCAATG 2 cut(s) 893, 1680
BsrFI RCCGGY 2 cut(s) 1810, 2609
BsrGI TGTACA 2 cut(s) 1840, 2437
BssAI RCCGGY 2 cut(s) 1810, 2609
BssECI CCNNGG 7 cut(s) 739, 991, 1060, 1805, 2462, 2808, 2957
BssHII GCGCGC 1 cut(s) 284
BssSI CACGAG 2 cut(s) 1560, 2523
BssT1I CCWWGG 3 cut(s) 739, 1805, 2808
Bst2BI CACGAG 2 cut(s) 1560, 2523
Bst4CI ACNGT 5 cut(s) 80, 1018, 1644, 2244, 2330
Bst6I CTCTTC 2 cut(s) 681, 761
BstAFI CTTAAG 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 1562
BstAUI TGTACA 2 cut(s) 1840, 2437
BstC8I GCNNGC 8 cut(s) 286, 1326, 1526, 1572, 2065, 2165, 2353, 2815
BstDSI CCRYGG 2 cut(s) 739, 2808
BstENI CCTNNNNNAGG 1 cut(s) 1005
BstF5I GGATG 8 cut(s) 89, 637, 792, 1276, 1705, 1877, 2103, 2440
BstFNI CGCG 2 cut(s) 286, 2187
BstHHI GCGC 5 cut(s) 286, 288, 588, 1672, 2189
BstMAI GTCTC 3 cut(s) 815, 1584, 2826
BstMWI GCNNNNNNNGC 6 cut(s) 283, 641, 803, 1562, 1571, 1664
BstNSI RCATGY 5 cut(s) 665, 1231, 1377, 2821, 2867
BstSFI CTRYAG 3 cut(s) 1026, 1098, 1455
BstSLI GKGCMC 3 cut(s) 916, 1751, 2470
BstUI CGCG 2 cut(s) 286, 2187
BstV1I GCAGC 8 cut(s) 286, 644, 1040, 1242, 1568, 1654, 1888, 2248
BstV2I GAAGAC 4 cut(s) 23, 338, 579, 914
BstX2I RGATCY 4 cut(s) 196, 358, 1390, 2271
BstYI RGATCY 4 cut(s) 196, 358, 1390, 2271
BsuRI GGCC 9 cut(s) 401, 861, 1059, 1403, 1810, 2355, 2413, 2733, 2779
BtgI CCRYGG 2 cut(s) 739, 2808
BtrI CACGTC 1 cut(s) 2600
BtsCI GGATG 8 cut(s) 89, 637, 792, 1276, 1705, 1877, 2103, 2440
BtsI GCAGTG 3 cut(s) 248, 819, 1519
BveI ACCTGC 2 cut(s) 1489, 1618
Cac8I GCNNGC 8 cut(s) 286, 1326, 1526, 1572, 2065, 2165, 2353, 2815
CaiI CAGNNNCTG 3 cut(s) 1463, 1655, 2242
CfoI GCGC 5 cut(s) 286, 288, 588, 1672, 2189
Cfr10I RCCGGY 2 cut(s) 1810, 2609
CsiI ACCWGGT 3 cut(s) 595, 916, 1209
Csp6I GTAC 6 cut(s) 593, 771, 1430, 1841, 2424, 2438
CviQI GTAC 6 cut(s) 593, 771, 1430, 1841, 2424, 2438
DraI TTTAAA 1 cut(s) 2848
DraIII CACNNNGTG 1 cut(s) 2948
EaeI YGGCCR 3 cut(s) 1401, 1808, 2353
Eam1104I CTCTTC 2 cut(s) 681, 761
EarI CTCTTC 2 cut(s) 681, 761
Ecl136II GAGCTC 1 cut(s) 931
Eco130I CCWWGG 3 cut(s) 739, 1805, 2808
Eco24I GRGCYC 1 cut(s) 933
Eco32I GATATC 1 cut(s) 2453
Eco47I GGWCC 9 cut(s) 4, 256, 883, 995, 1532, 1637, 2306, 2492, 2540
Eco53kI GAGCTC 1 cut(s) 931
Eco57I CTGAAG 3 cut(s) 730, 953, 1340
Eco88I CYCGRG 2 cut(s) 362, 2463
EcoICRI GAGCTC 1 cut(s) 931
EcoNI CCTNNNNNAGG 1 cut(s) 1005
EcoO109I RGGNCCY 2 cut(s) 256, 2492
EcoRV GATATC 1 cut(s) 2453
EcoT14I CCWWGG 3 cut(s) 739, 1805, 2808
EcoT22I ATGCAT 3 cut(s) 178, 940, 1707
EcoT38I GRGCYC 1 cut(s) 933
ErhI CCWWGG 3 cut(s) 739, 1805, 2808
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FaqI GGGAC 8 cut(s) 198, 242, 672, 1077, 1809, 1934, 1998, 2549
FauI CCCGC 2 cut(s) 1317, 2806
FauNDI CATATG 2 cut(s) 1978, 2010
FbaI TGATCA 1 cut(s) 1968
FblI GTMKAC 3 cut(s) 1101, 1445, 2678
Fnu4HI GCNGC 9 cut(s) 275, 611, 633, 1029, 1256, 1557, 1668, 1902, 2237
FokI GGATG 8 cut(s) 96, 624, 799, 1283, 1692, 1864, 2090, 2427
FriOI GRGCYC 1 cut(s) 933
Fsp4HI GCNGC 9 cut(s) 275, 611, 633, 1029, 1256, 1557, 1668, 1902, 2237
FspBI CTAG 7 cut(s) 1086, 1109, 1290, 1686, 1869, 1953, 2980
FspI TGCGCA 1 cut(s) 1671
GlaI GCGC 5 cut(s) 285, 287, 587, 1671, 2188
GluI GCNGC 9 cut(s) 275, 611, 633, 1029, 1256, 1557, 1668, 1902, 2237
GsaI CCCAGC 2 cut(s) 1569, 2431
GsuI CTGGAG 3 cut(s) 645, 1407, 2331
HaeIII GGCC 9 cut(s) 401, 861, 1059, 1403, 1810, 2355, 2413, 2733, 2779
HapII CCGG 5 cut(s) 371, 926, 1811, 2444, 2610
HhaI GCGC 5 cut(s) 286, 288, 588, 1672, 2189
Hin6I GCGC 5 cut(s) 284, 286, 586, 1670, 2187
HinP1I GCGC 5 cut(s) 284, 286, 586, 1670, 2187
HincII GTYRAC 4 cut(s) 675, 754, 1446, 2259
HindII GTYRAC 4 cut(s) 675, 754, 1446, 2259
HindIII AAGCTT 1 cut(s) 804
HinfI GANTC 7 cut(s) 488, 1442, 1447, 1474, 2072, 2145, 2260
HpaI GTTAAC 1 cut(s) 675
HpaII CCGG 5 cut(s) 371, 926, 1811, 2444, 2610
HphI GGTGA 2 cut(s) 611, 2849
Hpy99I CGWCG 1 cut(s) 2289
HpyAV CCTTC 8 cut(s) 110, 175, 977, 1132, 1211, 1261, 2692, 2703
HpyCH4III ACNGT 5 cut(s) 80, 1018, 1644, 2244, 2330
HpyCH4IV ACGT 3 cut(s) 1908, 2599, 2789
HpyF10VI GCNNNNNNNGC 6 cut(s) 283, 641, 803, 1562, 1571, 1664
HpySE526I ACGT 3 cut(s) 1908, 2599, 2789
HspAI GCGC 5 cut(s) 284, 286, 586, 1670, 2187
Kpn2I TCCGGA 1 cut(s) 925
KpnI GGTACC 1 cut(s) 2427
Ksp22I TGATCA 1 cut(s) 1968
KspAI GTTAAC 1 cut(s) 675
LmnI GCTCC 7 cut(s) 271, 794, 928, 1087, 2528, 2549, 2939
Lsp1109I GCAGC 8 cut(s) 286, 644, 1040, 1242, 1568, 1654, 1888, 2248
LweI GCATC 8 cut(s) 334, 406, 619, 777, 947, 1714, 2140, 2148
MabI ACCWGGT 3 cut(s) 595, 916, 1209
MaeI CTAG 7 cut(s) 1086, 1109, 1290, 1686, 1869, 1953, 2980
MaeII ACGT 3 cut(s) 1908, 2599, 2789
MaeIII GTNAC 6 cut(s) 508, 887, 1018, 1765, 1921, 2929
MfeI CAATTG 3 cut(s) 1032, 1262, 2619
MflI RGATCY 4 cut(s) 196, 358, 1390, 2271
MhlI GDGCHC 8 cut(s) 301, 916, 933, 1084, 1751, 2029, 2470, 2944
MlsI TGGCCA 2 cut(s) 1403, 2355
MluNI TGGCCA 2 cut(s) 1403, 2355
MlyI GAGTC 3 cut(s) 1441, 1451, 2254
MmeI TCCRAC 3 cut(s) 1492, 2527, 2543
Mox20I TGGCCA 2 cut(s) 1403, 2355
Mph1103I ATGCAT 3 cut(s) 178, 940, 1707
MroI TCCGGA 1 cut(s) 925
MscI TGGCCA 2 cut(s) 1403, 2355
MseI TTAA 9 cut(s) 164, 171, 252, 453, 534, 674, 1196, 2151, 2847
Msp20I TGGCCA 2 cut(s) 1403, 2355
MspA1I CMGCKG 1 cut(s) 2351
MspCI CTTAAG 1 cut(s) 163
MspI CCGG 5 cut(s) 371, 926, 1811, 2444, 2610
MunI CAATTG 3 cut(s) 1032, 1262, 2619
Mva1269I GAATGC 4 cut(s) 74, 1472, 1707, 2611
MvnI CGCG 2 cut(s) 286, 2187
MwoI GCNNNNNNNGC 6 cut(s) 283, 641, 803, 1562, 1571, 1664
NciI CCSGG 1 cut(s) 372
NcoI CCATGG 2 cut(s) 739, 2808
NdeI CATATG 2 cut(s) 1978, 2010
NlaIV GGNNCC 9 cut(s) 156, 258, 996, 1415, 1815, 2425, 2469, 2494, 2734
NmuCI GTSAC 3 cut(s) 508, 1018, 1921
NsbI TGCGCA 1 cut(s) 1671
NsiI ATGCAT 3 cut(s) 178, 940, 1707
NspI RCATGY 5 cut(s) 665, 1231, 1377, 2821, 2867
PaeR7I CTCGAG 1 cut(s) 362
PalAI GGCGCGCC 1 cut(s) 284
PauI GCGCGC 1 cut(s) 284
PciI ACATGT 4 cut(s) 661, 1227, 1373, 2863
PctI GAATGC 4 cut(s) 74, 1472, 1707, 2611
PfeI GAWTC 4 cut(s) 488, 1474, 2072, 2145
PflFI GACNNNGTC 1 cut(s) 758
PflMI CCANNNNNTGG 4 cut(s) 397, 2486, 2525, 2726
PfoI TCCNGGA 1 cut(s) 2308
PkrI GCNGC 9 cut(s) 276, 612, 634, 1030, 1257, 1558, 1669, 1903, 2238
PleI GAGTC 3 cut(s) 1441, 1450, 2254
PpsI GAGTC 3 cut(s) 1441, 1450, 2254
PpuMI RGGWCCY 2 cut(s) 256, 2492
PscI ACATGT 4 cut(s) 661, 1227, 1373, 2863
PsiI TTATAA 1 cut(s) 2046
Psp124BI GAGCTC 1 cut(s) 933
Psp1406I AACGTT 2 cut(s) 1908, 2789
Psp5II RGGWCCY 2 cut(s) 256, 2492
PspFI CCCAGC 2 cut(s) 1565, 2427
PspN4I GGNNCC 9 cut(s) 156, 258, 996, 1415, 1815, 2425, 2469, 2494, 2734
PspPPI RGGWCCY 2 cut(s) 256, 2492
PstI CTGCAG 1 cut(s) 1030
PstNI CAGNNNCTG 3 cut(s) 1463, 1655, 2242
PsuI RGATCY 4 cut(s) 196, 358, 1390, 2271
PsyI GACNNNGTC 1 cut(s) 758
PteI GCGCGC 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 2351
RsaI GTAC 6 cut(s) 594, 772, 1431, 1842, 2425, 2439
RsaNI GTAC 6 cut(s) 593, 771, 1430, 1841, 2424, 2438
SacI GAGCTC 1 cut(s) 933
SalI GTCGAC 1 cut(s) 1444
SaqAI TTAA 9 cut(s) 164, 171, 252, 453, 534, 674, 1196, 2151, 2847
SatI GCNGC 9 cut(s) 275, 611, 633, 1029, 1256, 1557, 1668, 1902, 2237
ScaI AGTACT 1 cut(s) 1431
SchI GAGTC 3 cut(s) 1441, 1451, 2254
SduI GDGCHC 8 cut(s) 301, 916, 933, 1084, 1751, 2029, 2470, 2944
SexAI ACCWGGT 3 cut(s) 595, 916, 1209
SfaNI GCATC 8 cut(s) 334, 406, 619, 777, 947, 1714, 2140, 2148
SfcI CTRYAG 3 cut(s) 1026, 1098, 1455
Sfr274I CTCGAG 1 cut(s) 362
SgsI GGCGCGCC 1 cut(s) 284
SinI GGWCC 9 cut(s) 4, 256, 883, 995, 1532, 1637, 2306, 2492, 2540
SlaI CTCGAG 1 cut(s) 362
SmlI CTYRAG 5 cut(s) 163, 362, 942, 1933, 2934
SmoI CTYRAG 5 cut(s) 163, 362, 942, 1933, 2934
SsiI CCGC 4 cut(s) 610, 1324, 2636, 2813
SspMI CTAG 7 cut(s) 1086, 1109, 1290, 1686, 1869, 1953, 2980
SstI GAGCTC 1 cut(s) 933
StyI CCWWGG 3 cut(s) 739, 1805, 2808
TaaI ACNGT 5 cut(s) 80, 1018, 1644, 2244, 2330
TaiI ACGT 3 cut(s) 1911, 2602, 2792
TaqI TCGA 5 cut(s) 363, 1396, 1445, 2015, 2191
TatI WGTACW 4 cut(s) 770, 1429, 1840, 2437
TauI GCSGC 1 cut(s) 613
TfiI GAWTC 4 cut(s) 488, 1474, 2072, 2145
Tru1I TTAA 9 cut(s) 164, 171, 252, 453, 534, 674, 1196, 2151, 2847
Tru9I TTAA 9 cut(s) 164, 171, 252, 453, 534, 674, 1196, 2151, 2847
TseFI GTSAC 3 cut(s) 508, 1018, 1921
TseI GCWGC 8 cut(s) 274, 632, 1028, 1255, 1556, 1667, 1901, 2236
Tsp45I GTSAC 3 cut(s) 508, 1018, 1921
TspGWI ACGGA 3 cut(s) 875, 1406, 2859
Tth111I GACNNNGTC 1 cut(s) 758
Van91I CCANNNNNTGG 4 cut(s) 397, 2486, 2525, 2726
Vha464I CTTAAG 1 cut(s) 163
VneI GTGCAC 1 cut(s) 912
VpaK11BI GGWCC 9 cut(s) 4, 256, 883, 995, 1532, 1637, 2306, 2492, 2540
XagI CCTNNNNNAGG 1 cut(s) 1005
XapI RAATTY 1 cut(s) 1721
XceI RCATGY 5 cut(s) 665, 1231, 1377, 2821, 2867
XcmI CCANNNNNNNNNTGG 2 cut(s) 998, 2723
XhoI CTCGAG 1 cut(s) 362
XmiI GTMKAC 3 cut(s) 1101, 1445, 2678
XspI CTAG 7 cut(s) 1086, 1109, 1290, 1686, 1869, 1953, 2980
ZrmI AGTACT 1 cut(s) 1431
Zsp2I ATGCAT 3 cut(s) 178, 940, 1707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.