MD16G1268400.v1.1

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009459
Physical Location & Seq
Forward (+)
34551621 .. 34564944
13324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1268400.v1.1.491

Sequence Viewer

Length: 3843 bp
ATGGATTCACAGCCTAACAACCTCTTTGACACGGCGTCGCAGCCCGACACCGCCAATGACGCCTACACCTTCCTCGAATTCAACACCCAAGGCGAGGACCTTGACTACCCGGAGTTCCGCGACCCGATTCGGTCCCCTGTCGCCTGGCCCACCGCTTCAGATTCCTTATCGGAACCCACTGACCGTGACCGTGGCGGGGGTGGAGACCACCAGCCTGATGCATCACCGGTTTCAGCTGCCCCTGGCAGCGCCACAAAGGCTAGGGCAGGTGGGTCGGGCAGTAAGGCCGGGAATAATCAGGTGGTTGACGTCCCAACGGCTGGGATGAGTGTGCTTAACTTTGAGGACACTGGGGATGACGACAATTACGAGTTTGGAAAAGGGGATTTCACGGAACATGCGTGCCGGTACTGTGGCGTGTCGAACCCGGCGTGCGTTGTGAGGTGTAATGTGCCTTCGTGTCGAAAATGGTTCTGTAATTCAAGGGGGAACACGTCCGGCTCCCATATTGTGAATCACCTGGTAATTCACCTGGTCCGAGCCAAACACAAGGAAGTCTGTCTTCATAAAGATAGCCCATTGGGAGAAACAATCCTTGAATGCTACAACTGTGGATGTCGTAATGTATTCCTTCTGGGATTTATTTCAGCCAAGACAGAGAGTGTTGTTGTTCTTCTTTGTAGGGAACCCTGCTTAAGTGTTAATGCATTGAAGGATATGAACTGGGATCTGAGCCAGTGGTGTCCCCTGATAGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGGGTTTTTAAGATGCTGTGCTTTGCAGGTCCCGTCTGAACAGGAGCAGCTAAGGGCGCGCCAAATCAGTGCTCAACAAATAAATAAAGTAGAAGAACTTTGGAAGACCAATCCTGATGCTTCCCTTGAAGATCTCGAGAAGCCGGGTGTGGACGATGAACCCCAACCTGTGGCCTTGAAGTATGAAGATGCTTACCAGTATCAAAATGTGTTTGCACCACTTATTAAACTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGTAAAGATAATGTCACAATTCGGTGGGATATTGGTCTTAATAAGAAGCGTATTGCTTATTTTGTGTTTACAAAGGAAGACAATGAATTGCGCCTTGTACCTGGTGATGAGTTGCGGCTTCGTTATTCTGGAGATGCAGCTCATCCAGCATGGCAGTCAGTGGGACATGTTATCAAGTTAACTCCACAAGAAGAGGTTGCACTTGAACTTCGTGCCAGTCAGGGTGTTCCAGTTGATCTGAACCATGGCTTCAGTGTTGACTTTGTCTGGAAGAGTACAAGTTTTGATCGGATGCAGGGAGCTATGAAAGCTTTTGCAGTGGACGAGACAAGTGTTAGTGGATATATTTATCATCATTTGTTAGGCCATGAAGTAGAGGTTCAAATGGTCCGTAACACATTGCCTCGCCGTTTTGGTGCACCTGGTCTTCCGGAGCTCAATGCATCTCAAAGTGTCCTTCAGAAGCCTATAAGTTTGATCCAGGGTCCACCTGGCACAGGAAAAACTGTGACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGCCAGGGACAGGTTTTGGTGTGTGCTCCTAGTAATGTTGCTGTAGACCAACTAGCTGAGAAGATAAGTGCAACTGGGTTGAAGGTTGTTAGACTTTGTGCAAAATCAAGGGAAGCCGTAAGTTCTCCTGTTGAGCATTTAACCCTTCATTACCAGGTTCGTCATCTTGACATGTCAGAAAAGAGTGAACTTCACAAGCTGCAACAATTGAAGGATGAGCAAGGAGAGTTGTCTAGTAATGATGAGAAAAAATATAAGGCACTGAAGCGGGCAACCGAGAGGGAGATTTCTCAGAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGAGATCCTCGATTGGCCAATTTTAGGTTCCGTCAGGTACTTATTGATGAGTCGACTCAGGCTACAGAACCTGAATGCCTGATTCCTTTGGTTCTTGGGGCAAAGCAGGTTGTTCTTGTTGGAGATCACTGCCAGCTTGGTCCAGTCATTATGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTTTTTGAACGCCTCGTTATACTTGGTGTGAAACCAATTAGATTGCAGGTTCAATACCGAATGCATCCAGCCTTGTCAGAATTTCCATCTAACAGCTTCTACGAGGGCACACTTCAAAATGGAGTTACCATCAATGAAAGGCAATCATCGGGCATTGATTTTCCTTGGCCGGTTCCCAATCGTCCCATGTTCTTTTATGTACAGATGGGGCAAGAGGAGATAAGTGCTAGTGGAACATCCTATCTGAACAGAACTGAGGCTGCTAACGTTGAAAAGATTGTCACAACATTCTTGAGGAGTGGCGTAGTCCCTAGTCAGATTGGAGTGATCACACCATATGAGGGACAGAGAGCTTATATTGTGAACCATATGTCGAGAAATGGTGCTCTCAGACAGCAACTTTATAAGGAAATTGAGGTTGCCAGCGTAGATTCATTTCAAGGACGGGAAAAGGATTATATCATCCTTTCGTGTGTAAGGAGTAATGAGCATCAGGGCATCGGATTCCTTAATGATCCTCGCAGGCTCAATGTTGCTCTAACACGCGCTCGATATGGTATTGTCATTCTTGGAAACCCTAAAGTTCTTAGTAAGCAGCCACTGTGGAATAGCCTGTTGACTCATTACAAGGAACACGAGTGCTTGGTTGAGGGGCCGCTAAATAACCTGAAGCAGAGTATGGTGCAATTTCCAAAGCCTAAAAAGATCTACAATGATCGTCGCCTATTCTATGGTGGTGGTCCTGGAGTTATCCCCAATGACAGTTATGGGTCTGTTGCCTCAGCTGGCCAAAGTGCTGATAGAAGAAGTAATCGGGGTAGGGGTTCTTACCTACCTCCTGGCCCACCCAATGGTACCCAGCCTGGTGTACATCCGGCTGGATATCCATTACCTCGGGCACCTCTTTCGCCATTTCATGGAGGACCCCTTTCTCAACCTTATGCTATTCCAACTCGTGGAGCTGTGCATGGACCAGTTGGAGCTGTTCCTCATGTCCCTCAACCTGGAAGTCGTGGTTTTGGGGCAGGACGTGGGAATGCCGGTTCTCCAATTGGCAGTCATCTTCCGCACCAGCAAGGCACACAACAAAATGTTGGGAATATGGGGTCTACCTTCAACATTCCTTCTCTTGAAAATCCAAATAGCCAACCATCTGTTGGTGGCCCCTTATCTCAACATGGATTTGTCAATAATATGCCAGTTCAAGGGCCAAGCCAAACGTTTCGTGATGGATTTTCCATGGCGGGCATGTCTCAGGAATGTTTGGGTGATGACTTTAAAAGTCAGGGATCACATGTTCCGTATAATGTTGCTGACTTCTCCACTCAGGCTTCTCAAAGTGGATATGGTGTTGATTATGTTACTCAAGGAGCACAAGGTGGGTTTCCAGGGAACTTTCTGAACCAGAACTCTCAAGCTGGATATTCTCGATTTGGTACGGGGAATGACTTCATGTCTCAGGACTACATGCCTCATGGATCACAAGGCCTATTTACTCAGGTGGGCTTTAATGACCCATCACAGGATGAAGCATCACAGAACCACTATGGAGTCGCAAATGCCAATCAACTTCAGTCTCAGGGCTTTATGAACTCACTGTACTCTCAGCCTTTTGCCCATTACAACACACAGCCAATGAACTTGCAGGCCCCACAACAACAACAACAGCAGCAACAGCTGCAGGGTCAGACCTCCCAAAATCAGAAACTCCACTACAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

1281

Amino Acids

140.56

Weight (kDa)

6.38

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 133 - 299 1.3e-65 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 351 - 440 1.7e-26 RNA helicase UPF1, 1B domain
AAA_11 PF13086 498 - 611 3.8e-21 AAA domain
AAA_30 PF13604 499 - 692 1.6e-11 AAA domain
AAA_19 PF13245 499 - 692 1.8e-10 AAA domain
AAA_11 PF13086 593 - 694 8.3e-29 AAA domain
AAA_12 PF13087 703 - 899 1.6e-61 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2523
AarI CACCTGC 1 cut(s) 257
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 4 cut(s) 257, 802, 2026, 2155
Acc65I GGTACC 1 cut(s) 2972
AccB1I GGYRCC 2 cut(s) 2972, 3016
AccB7I CCANNNNNTGG 6 cut(s) 320, 955, 2969, 3035, 3074, 3275
AccI GTMKAC 3 cut(s) 1644, 1982, 3227
AccII CGCG 3 cut(s) 120, 844, 2664
AccIII TCCGGA 1 cut(s) 1483
AciI CCGC 9 cut(s) 51, 118, 153, 195, 1168, 1867, 2774, 3185, 3362
AclI AACGTT 2 cut(s) 2385, 3338
AclWI GGATC 6 cut(s) 735, 1525, 1928, 2627, 3415, 3604
AcoI YGGCCR 3 cut(s) 1944, 2285, 2905
AcsI RAATTY 2 cut(s) 77, 2198
AcuI CTGAAG 6 cut(s) 141, 1288, 1496, 1883, 2807, 3674
AcyI GRCGYC 3 cut(s) 35, 60, 309
AdeI CACNNNGTG 1 cut(s) 3497
AfaI GTAC 9 cut(s) 410, 1152, 1330, 1968, 2319, 2974, 2988, 3556, 3719
AflII CTTAAG 1 cut(s) 694
AflIII ACRYGT 5 cut(s) 492, 1219, 1770, 1916, 3412
AgeI ACCGGT 1 cut(s) 226
AhdI GACNNNNNGTC 2 cut(s) 34, 3571
AjiI CACGTC 2 cut(s) 495, 3149
AjuI GAANNNNNNNTTGG 2 cut(s) 1940, 1972
AloI GAACNNNNNNTCC 4 cut(s) 3145, 3177, 3399, 3431
Alw21I GWGCWC 6 cut(s) 859, 1474, 1491, 1627, 2506, 3493
Alw26I GTCTC 6 cut(s) 198, 1373, 2121, 3375, 3579, 3699
Alw44I GTGCAC 1 cut(s) 1470
AlwI GGATC 6 cut(s) 735, 1525, 1928, 2627, 3415, 3604
AlwNI CAGNNNCTG 2 cut(s) 2000, 2719
Ama87I CYCGRG 2 cut(s) 920, 3012
Aor13HI TCCGGA 1 cut(s) 1483
ApaLI GTGCAC 1 cut(s) 1470
ApoI RAATTY 2 cut(s) 77, 2198
AscI GGCGCGCC 1 cut(s) 842
AsiGI ACCGGT 1 cut(s) 226
Asp700I GAANNNNTTC 1 cut(s) 3566
Asp718I GGTACC 1 cut(s) 2972
AspLEI GCGC 5 cut(s) 251, 844, 846, 1146, 2666
AsuC2I CCSGG 4 cut(s) 110, 289, 428, 930
AsuHPI GGTGA 5 cut(s) 216, 509, 521, 1169, 3398
AvaI CYCGRG 2 cut(s) 920, 3012
BaeGI GKGCMC 3 cut(s) 1474, 2228, 3019
BalI TGGCCA 2 cut(s) 1946, 2907
BanI GGYRCC 2 cut(s) 2972, 3016
BanII GRGCYC 1 cut(s) 1491
BarI GAAGNNNNNNTAC 2 cut(s) 439, 471
BauI CACGAG 3 cut(s) 2097, 2753, 3072
BbsI GAAGAC 4 cut(s) 554, 896, 1137, 1472
Bbv12I GWGCWC 6 cut(s) 859, 1474, 1491, 1627, 2506, 3493
BbvCI CCTCAGC 1 cut(s) 2899
BccI CCATC 6 cut(s) 2212, 2255, 2317, 3277, 3341, 3643
BceAI ACGGC 4 cut(s) 48, 333, 1446, 1700
BcgI CGANNNNNNTGC 4 cut(s) 255, 289, 3006, 3040
BclI TGATCA 1 cut(s) 2445
BcnI CCSGG 4 cut(s) 110, 289, 428, 930
BcoDI GTCTC 6 cut(s) 198, 1373, 2121, 3375, 3579, 3699
BfaI CTAG 6 cut(s) 261, 1629, 1652, 1833, 2346, 2430
BfmI CTRYAG 4 cut(s) 1569, 1641, 1992, 3797
BfoI RGCGCY 1 cut(s) 252
BfrI CTTAAG 1 cut(s) 694
BfuAI ACCTGC 4 cut(s) 257, 802, 2026, 2155
BglI GCCNNNNNGGC 1 cut(s) 257
BglII AGATCT 2 cut(s) 916, 2823
BmeRI GACNNNNNGTC 2 cut(s) 34, 3571
BmeT110I CYCGRG 2 cut(s) 920, 3012
BmgBI CACGTC 2 cut(s) 495, 3149
BmrI ACTGGG 4 cut(s) 360, 733, 1683, 2107
BmuI ACTGGG 4 cut(s) 360, 733, 1683, 2107
BpiI GAAGAC 4 cut(s) 554, 896, 1137, 1472
BpmI CTGGAG 3 cut(s) 1203, 1950, 2883
Bpu10I CCTNAGC 2 cut(s) 836, 2899
BpuEI CTTGAG 3 cut(s) 2431, 3468, 3516
BpuMI CCSGG 4 cut(s) 110, 289, 428, 930
BsaHI GRCGYC 3 cut(s) 35, 60, 309
BsaI GGTCTC 1 cut(s) 198
BsaWI WCCGGW 2 cut(s) 226, 1483
Bse118I RCCGGY 4 cut(s) 226, 405, 2287, 3158
Bse3DI GCAATG 1 cut(s) 1451
BseAI TCCGGA 1 cut(s) 1483
BseMI GCAATG 1 cut(s) 1451
BsePI GCGCGC 1 cut(s) 842
BseRI GAGGAG 2 cut(s) 2348, 2428
BseSI GKGCMC 3 cut(s) 1474, 2228, 3019
BseYI CCCAGC 3 cut(s) 320, 2102, 2976
Bsh1236I CGCG 3 cut(s) 120, 844, 2664
BshNI GGYRCC 2 cut(s) 2972, 3016
BshTI ACCGGT 1 cut(s) 226
BsiHKAI GWGCWC 6 cut(s) 859, 1474, 1491, 1627, 2506, 3493
BsiHKCI CYCGRG 2 cut(s) 920, 3012
BsmAI GTCTC 6 cut(s) 198, 1373, 2121, 3375, 3579, 3699
BsmI GAATGC 4 cut(s) 605, 2009, 2184, 3160
Bso31I GGTCTC 1 cut(s) 198
BsoBI CYCGRG 2 cut(s) 920, 3012
Bsp1286I GDGCHC 8 cut(s) 859, 1474, 1491, 1627, 2228, 2506, 3019, 3493
Bsp13I TCCGGA 1 cut(s) 1483
Bsp1407I TGTACA 2 cut(s) 2317, 2986
Bsp19I CCATGG 2 cut(s) 1297, 3357
BspACI CCGC 9 cut(s) 51, 118, 153, 195, 1168, 1867, 2774, 3185, 3362
BspEI TCCGGA 1 cut(s) 1483
BspFNI CGCG 3 cut(s) 120, 844, 2664
BspMAI CTGCAG 2 cut(s) 1573, 3801
BspMI ACCTGC 4 cut(s) 257, 802, 2026, 2155
BspPI GGATC 6 cut(s) 735, 1525, 1928, 2627, 3415, 3604
BspT107I GGYRCC 2 cut(s) 2972, 3016
BspTI CTTAAG 1 cut(s) 694
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 1451
BsrFI RCCGGY 4 cut(s) 226, 405, 2287, 3158
BsrGI TGTACA 2 cut(s) 2317, 2986
BssAI RCCGGY 4 cut(s) 226, 405, 2287, 3158
BssHII GCGCGC 1 cut(s) 842
BssNI GRCGYC 3 cut(s) 35, 60, 309
BssSI CACGAG 3 cut(s) 2097, 2753, 3072
BssT1I CCWWGG 4 cut(s) 88, 1297, 2282, 3357
Bst2BI CACGAG 3 cut(s) 2097, 2753, 3072
Bst4CI ACNGT 8 cut(s) 185, 191, 413, 611, 1561, 2721, 2882, 3717
Bst6I CTCTTC 2 cut(s) 1239, 1319
BstACI GRCGYC 3 cut(s) 35, 60, 309
BstAFI CTTAAG 1 cut(s) 694
BstAPI GCANNNNNTGC 2 cut(s) 2099, 3796
BstAUI TGTACA 2 cut(s) 2317, 2986
BstDSI CCRYGG 3 cut(s) 190, 1297, 3357
BstENI CCTNNNNNAGG 1 cut(s) 1548
BstFNI CGCG 3 cut(s) 120, 844, 2664
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 5 cut(s) 251, 844, 846, 1146, 2666
BstMAI GTCTC 6 cut(s) 198, 1373, 2121, 3375, 3579, 3699
BstMWI GCNNNNNNNGC 9 cut(s) 59, 257, 841, 1199, 1361, 2099, 2108, 3793, 3796
BstNSI RCATGY 7 cut(s) 401, 1223, 1774, 1920, 3370, 3416, 3589
BstSFI CTRYAG 4 cut(s) 1569, 1641, 1992, 3797
BstSLI GKGCMC 3 cut(s) 1474, 2228, 3019
BstUI CGCG 3 cut(s) 120, 844, 2664
BstV2I GAAGAC 4 cut(s) 554, 896, 1137, 1472
BstX2I RGATCY 4 cut(s) 727, 916, 1933, 2823
BstXI CCANNNNNNTGG 1 cut(s) 3836
BstYI RGATCY 4 cut(s) 727, 916, 1933, 2823
BtgI CCRYGG 3 cut(s) 190, 1297, 3357
BtrI CACGTC 2 cut(s) 495, 3149
BtsI GCAGTG 3 cut(s) 779, 1377, 2056
BveI ACCTGC 4 cut(s) 257, 802, 2026, 2155
CaiI CAGNNNCTG 2 cut(s) 2000, 2719
CfoI GCGC 5 cut(s) 251, 844, 846, 1146, 2666
Cfr10I RCCGGY 4 cut(s) 226, 405, 2287, 3158
CseI GACGC 2 cut(s) 24, 68
CsiI ACCWGGT 5 cut(s) 519, 531, 1153, 1474, 1752
Csp6I GTAC 9 cut(s) 409, 1151, 1329, 1967, 2318, 2973, 2987, 3555, 3718
CspAI ACCGGT 1 cut(s) 226
CviQI GTAC 9 cut(s) 409, 1151, 1329, 1967, 2318, 2973, 2987, 3555, 3718
DraI TTTAAA 1 cut(s) 3397
DraIII CACNNNGTG 1 cut(s) 3497
DriI GACNNNNNGTC 2 cut(s) 34, 3571
EaeI YGGCCR 3 cut(s) 1944, 2285, 2905
Eam1104I CTCTTC 2 cut(s) 1239, 1319
Eam1105I GACNNNNNGTC 2 cut(s) 34, 3571
EarI CTCTTC 2 cut(s) 1239, 1319
Ecl136II GAGCTC 1 cut(s) 1489
Eco130I CCWWGG 4 cut(s) 88, 1297, 2282, 3357
Eco147I AGGCCT 1 cut(s) 3606
Eco24I GRGCYC 1 cut(s) 1491
Eco31I GGTCTC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 3002
Eco53kI GAGCTC 1 cut(s) 1489
Eco57I CTGAAG 6 cut(s) 141, 1288, 1496, 1883, 2807, 3674
Eco88I CYCGRG 2 cut(s) 920, 3012
EcoICRI GAGCTC 1 cut(s) 1489
EcoNI CCTNNNNNAGG 1 cut(s) 1548
EcoO109I RGGNCCY 4 cut(s) 97, 814, 3041, 3766
EcoRI GAATTC 1 cut(s) 77
EcoRV GATATC 1 cut(s) 3002
EcoT14I CCWWGG 4 cut(s) 88, 1297, 2282, 3357
EcoT22I ATGCAT 4 cut(s) 223, 709, 1498, 2184
EcoT38I GRGCYC 1 cut(s) 1491
ErhI CCWWGG 4 cut(s) 88, 1297, 2282, 3357
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FauI CCCGC 3 cut(s) 188, 1860, 3355
FauNDI CATATG 2 cut(s) 2455, 2487
FbaI TGATCA 1 cut(s) 2445
FblI GTMKAC 3 cut(s) 1644, 1982, 3227
FriOI GRGCYC 1 cut(s) 1491
FspBI CTAG 6 cut(s) 261, 1629, 1652, 1833, 2346, 2430
GlaI GCGC 5 cut(s) 250, 843, 845, 1145, 2665
GsaI CCCAGC 3 cut(s) 324, 2106, 2980
GsuI CTGGAG 3 cut(s) 1203, 1950, 2883
HaeII RGCGCY 1 cut(s) 252
HgaI GACGC 2 cut(s) 24, 68
HhaI GCGC 5 cut(s) 251, 844, 846, 1146, 2666
Hin1I GRCGYC 3 cut(s) 35, 60, 309
Hin6I GCGC 5 cut(s) 249, 842, 844, 1144, 2664
HinP1I GCGC 5 cut(s) 249, 842, 844, 1144, 2664
HincII GTYRAC 5 cut(s) 307, 1233, 1312, 1983, 2736
HindII GTYRAC 5 cut(s) 307, 1233, 1312, 1983, 2736
HindIII AAGCTT 1 cut(s) 1362
HpaI GTTAAC 1 cut(s) 1233
HphI GGTGA 5 cut(s) 216, 509, 521, 1169, 3398
Hpy99I CGWCG 2 cut(s) 40, 2841
HpyCH4III ACNGT 8 cut(s) 185, 191, 413, 611, 1561, 2721, 2882, 3717
HpyCH4IV ACGT 5 cut(s) 309, 494, 2385, 3148, 3338
HpyF10VI GCNNNNNNNGC 9 cut(s) 59, 257, 841, 1199, 1361, 2099, 2108, 3793, 3796
HpySE526I ACGT 5 cut(s) 309, 494, 2385, 3148, 3338
Hsp92I GRCGYC 3 cut(s) 35, 60, 309
HspAI GCGC 5 cut(s) 249, 842, 844, 1144, 2664
Kpn2I TCCGGA 1 cut(s) 1483
KpnI GGTACC 1 cut(s) 2976
Ksp22I TGATCA 1 cut(s) 2445
KspAI GTTAAC 1 cut(s) 1233
LmnI GCTCC 8 cut(s) 506, 829, 1352, 1486, 1630, 3077, 3098, 3488
MabI ACCWGGT 5 cut(s) 519, 531, 1153, 1474, 1752
MaeI CTAG 6 cut(s) 261, 1629, 1652, 1833, 2346, 2430
MaeII ACGT 5 cut(s) 309, 494, 2385, 3148, 3338
MaeIII GTNAC 7 cut(s) 185, 1066, 1445, 1561, 2242, 2398, 3478
MfeI CAATTG 3 cut(s) 1575, 1805, 3168
MflI RGATCY 4 cut(s) 727, 916, 1933, 2823
MhlI GDGCHC 8 cut(s) 859, 1474, 1491, 1627, 2228, 2506, 3019, 3493
MlsI TGGCCA 2 cut(s) 1946, 2907
MluNI TGGCCA 2 cut(s) 1946, 2907
MlyI GAGTC 4 cut(s) 1978, 1988, 2731, 3678
MmeI TCCRAC 3 cut(s) 2029, 3076, 3092
Mox20I TGGCCA 2 cut(s) 1946, 2907
Mph1103I ATGCAT 4 cut(s) 223, 709, 1498, 2184
MroI TCCGGA 1 cut(s) 1483
MroXI GAANNNNTTC 1 cut(s) 3566
MscI TGGCCA 2 cut(s) 1946, 2907
MslI CAYNNNNRTG 1 cut(s) 3834
Msp20I TGGCCA 2 cut(s) 1946, 2907
MspA1I CMGCKG 3 cut(s) 236, 2903, 3796
MspCI CTTAAG 1 cut(s) 694
MunI CAATTG 3 cut(s) 1575, 1805, 3168
Mva1269I GAATGC 4 cut(s) 605, 2009, 2184, 3160
MvnI CGCG 3 cut(s) 120, 844, 2664
MwoI GCNNNNNNNGC 9 cut(s) 59, 257, 841, 1199, 1361, 2099, 2108, 3793, 3796
NciI CCSGG 4 cut(s) 110, 289, 428, 930
NcoI CCATGG 2 cut(s) 1297, 3357
NdeI CATATG 2 cut(s) 2455, 2487
NmuCI GTSAC 4 cut(s) 185, 1066, 1561, 2398
NsiI ATGCAT 4 cut(s) 223, 709, 1498, 2184
NspI RCATGY 7 cut(s) 401, 1223, 1774, 1920, 3370, 3416, 3589
PaeR7I CTCGAG 1 cut(s) 920
PalAI GGCGCGCC 1 cut(s) 842
PaqCI CACCTGC 1 cut(s) 257
PauI GCGCGC 1 cut(s) 842
PceI AGGCCT 1 cut(s) 3606
PciI ACATGT 4 cut(s) 1219, 1770, 1916, 3412
PcsI WCGNNNNNNNCGW 3 cut(s) 366, 398, 428
PctI GAATGC 4 cut(s) 605, 2009, 2184, 3160
PdmI GAANNNNTTC 1 cut(s) 3566
PfeI GAWTC 8 cut(s) 5, 127, 161, 514, 1046, 2011, 2549, 2622
PflFI GACNNNGTC 1 cut(s) 1316
PflMI CCANNNNNTGG 6 cut(s) 320, 955, 2969, 3035, 3074, 3275
PfoI TCCNGGA 1 cut(s) 2860
PinAI ACCGGT 1 cut(s) 226
PleI GAGTC 4 cut(s) 1978, 1987, 2731, 3677
PpsI GAGTC 4 cut(s) 1978, 1987, 2731, 3677
PpuMI RGGWCCY 3 cut(s) 97, 814, 3041
PscI ACATGT 4 cut(s) 1219, 1770, 1916, 3412
PsiI TTATAA 1 cut(s) 2523
Psp124BI GAGCTC 1 cut(s) 1491
Psp1406I AACGTT 2 cut(s) 2385, 3338
Psp5II RGGWCCY 3 cut(s) 97, 814, 3041
PspFI CCCAGC 3 cut(s) 320, 2102, 2976
PspPPI RGGWCCY 3 cut(s) 97, 814, 3041
PsrI GAACNNNNNNTAC 2 cut(s) 3701, 3733
PstI CTGCAG 2 cut(s) 1573, 3801
PstNI CAGNNNCTG 2 cut(s) 2000, 2719
PsuI RGATCY 4 cut(s) 727, 916, 1933, 2823
PsyI GACNNNGTC 1 cut(s) 1316
PteI GCGCGC 1 cut(s) 842
PvuII CAGCTG 3 cut(s) 236, 2903, 3796
RsaI GTAC 9 cut(s) 410, 1152, 1330, 1968, 2319, 2974, 2988, 3556, 3719
RsaNI GTAC 9 cut(s) 409, 1151, 1329, 1967, 2318, 2973, 2987, 3555, 3718
RseI CAYNNNNRTG 1 cut(s) 3834
SacI GAGCTC 1 cut(s) 1491
SalI GTCGAC 1 cut(s) 1981
SchI GAGTC 4 cut(s) 1978, 1988, 2731, 3678
SduI GDGCHC 8 cut(s) 859, 1474, 1491, 1627, 2228, 2506, 3019, 3493
SexAI ACCWGGT 5 cut(s) 519, 531, 1153, 1474, 1752
SfcI CTRYAG 4 cut(s) 1569, 1641, 1992, 3797
Sfr274I CTCGAG 1 cut(s) 920
SgsI GGCGCGCC 1 cut(s) 842
SlaI CTCGAG 1 cut(s) 920
SmiMI CAYNNNNRTG 1 cut(s) 3834
SmlI CTYRAG 5 cut(s) 694, 920, 2410, 3483, 3531
SmoI CTYRAG 5 cut(s) 694, 920, 2410, 3483, 3531
SseBI AGGCCT 1 cut(s) 3606
SsiI CCGC 9 cut(s) 51, 118, 153, 195, 1168, 1867, 2774, 3185, 3362
SspMI CTAG 6 cut(s) 261, 1629, 1652, 1833, 2346, 2430
SstI GAGCTC 1 cut(s) 1491
StuI AGGCCT 1 cut(s) 3606
StyI CCWWGG 4 cut(s) 88, 1297, 2282, 3357
TaaI ACNGT 8 cut(s) 185, 191, 413, 611, 1561, 2721, 2882, 3717
TaiI ACGT 5 cut(s) 312, 497, 2388, 3151, 3341
TaqI TCGA 9 cut(s) 75, 422, 463, 921, 1939, 1982, 2492, 2668, 3547
TaqII GACCGA 1 cut(s) 120
TatI WGTACW 4 cut(s) 1328, 2317, 2986, 3717
TauI GCSGC 2 cut(s) 1171, 2776
TfiI GAWTC 8 cut(s) 5, 127, 161, 514, 1046, 2011, 2549, 2622
TseFI GTSAC 4 cut(s) 185, 1066, 1561, 2398
Tsp45I GTSAC 4 cut(s) 185, 1066, 1561, 2398
TspGWI ACGGA 4 cut(s) 407, 1433, 1949, 3408
Tth111I GACNNNGTC 1 cut(s) 1316
Van91I CCANNNNNTGG 6 cut(s) 320, 955, 2969, 3035, 3074, 3275
Vha464I CTTAAG 1 cut(s) 694
VneI GTGCAC 1 cut(s) 1470
XagI CCTNNNNNAGG 1 cut(s) 1548
XapI RAATTY 2 cut(s) 77, 2198
XceI RCATGY 7 cut(s) 401, 1223, 1774, 1920, 3370, 3416, 3589
XcmI CCANNNNNNNNNTGG 2 cut(s) 1541, 3272
XhoI CTCGAG 1 cut(s) 920
XmiI GTMKAC 3 cut(s) 1644, 1982, 3227
XmnI GAANNNNTTC 1 cut(s) 3566
XspI CTAG 6 cut(s) 261, 1629, 1652, 1833, 2346, 2430
ZraI GACGTC 1 cut(s) 310
Zsp2I ATGCAT 4 cut(s) 223, 709, 1498, 2184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.