MD06G1042600.v1.1

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009459
Physical Location & Seq
Forward (+)
5583449 .. 5596287
12839 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1042600.v1.1.491

Sequence Viewer

Length: 3858 bp
ATGGATTCCGAGCCCAACAACCTCTTTGACACGGCGTCGCAGCCCGACACCTCCAACGACGCCTACACCTTCCTCGAATTCAACACCCAGGGCGAGGACTTTGACTACCCGGAGTTCCGCGACCCGATTCGCTCTCCTGTCGCCTGGCCCACTCCTTCAGATTCCTTATCGGAACCTGCAGACCGTGACCGTGGCGGTGGTGGGGTCGGGTCGGACCACCAGTCCGATGCGTCGCCGGTTTCTGCTGCCCCGGGCAGCGCCACAAAGGCTAGGGCAGGTGGGTCGGGCAGTAACGCCGGCAATAATCAGGTGGTTGACGGCCTAACGGCTGGGATGAGTGTGCTTAACTTTGAGGATACTGGGGATGACGACAATTACGAGTTTGGAAAAGGGGATTTCACAGAACATGCGTGCCGGTACTGCGGCGTGTCGAACCCGGCTTGCGTTGTGAGGTGTAATGTGCCTTCGTGTCGAAAATGGTTCTGTAATTCAAGGGGGAACACGTCCGGCTCTCATATTGTGAATCACCTGGTCCGAGCAAAACACAAGGAAGTCTGTCTTCATAAAGATAGCCCATTGGGAGAAACAATCCTTGAATGCTACAACTGTGGATGTCGTAATGTATTCCTTCTTGGATTTATTTCAGCCAAGACAGAGAGTGTTGTTGTTCTTCTTTGTAGGGAACCCTGCTTAAGTGTTAATGCATTGAAGGATATGAACTGGGATCTGAGCCAGTGGTGTCCCCTGATAGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGTCCCGTCTGAACAGGAGCAGCTAAGGGCACGCCAAATCAGTGCTCAACAAATTAATAAAGTAGAAGAACTTTGGAAGTCCAATCCTGATGCTTCCCTTGAAGATCTTGAGAAGCCTGGTGTGGATGATGAACCCCAACCTGTGGCCTTGAAGTATGAAGATGCTTACCAGTATCAAAATGTGTTTGCACCACTTATTAAACTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGCAAAGACAATGTCACAATTCGGTGGGATATTGGTCTTAACAAGAAGCGTATTGCTTATTTTGTGTTTCCAAAGGAAGACAATGAGTTACGTCTTGTACCTGGTGATGAGTTGCGGCTTCGTTATTCTGGAGATGCAGCTCATCCAGCATGGCAGTCAGTGGGACATGTTATCAAGTTAACTGCACAAGAGGAGGTTGCACTTGAACTTCGTGCCAGTCAGGGAGTTCCAGTTGATGTGAACCATGGCTTCAGTGTTGACTTTGTCTGGAAGAGTACAAGTTTTGATCGGATGCAGGGGGCCATGAAAGCTTTTGCAGTGGACGAGACAAGTGTTAGTGGATATATTTACCATCATTTGTTAGGCCATGAAGTAGAGGTTCAAATGGTCCGTAATACATTGCCCCGTCGTTTTGGTGCACCTGGTCTTCCGGAGCTCAATGCATCTCAAGTTTTTGCTGTAAAGAGTGTCCTTCAGAAGCCTATAAGTTTGATCCAGGGTCCACCTGGCACAGGAAAAACTGTGACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGCCAGGGACAGGTTTTGGTCTGTGCTCCTAGTAATGTTGCTGTAGACCAACTAGCTGAGAAGATAAGTGCAACTGGGTTGAAGGTTGTTAGACTTTGTGCAAAATCAAGGGAAGCTGTTAGTTCTCCTGTTGAGCATTTAACCCTTCATTACCAGGTTCGTCATCTTGACACGTCAGAAAAGAGTGAACTGTACAAGCTACAACAATTGAAGGATGAGCAAGGAGAATTGTCTAGTAGCGATGAGAAAAAATATAAGGCACTGAAGCGGGCAACCGAGAGGGAGATTTCTCAAAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGAGATCCACGATTGGCCAATTTTAGGTTCCGTCAGGTACTTATTGATGAGTCTACTCAGTCTACGGAGCCTGAATGCCTGATTCCTTTGGTTCTTGGGGCAAAGCAGGTTGTTCTTGTTGGAGATCACTGCCAGCTTGGTCCAGTCATTATGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTCTTTGAACGCCTCGTTTTACTTGGTGTGAAACCAATTAGATTGCAGGTTCAATACCGAATGCATCCAGCCTTGTCAGAATTTCCATCTAATAGCTTCTATGAGGGCACGCTTCAAAATGGAGTGACCATCAATGAAAGGCAATCATCAGGCATTGATTTTCCTTGGCCGGTTCCCAATCGTCCCATGTTCTTTTATGTACAGATGGGCCAAGAGGAGATAAGTGCTAGTGGAACATCCTATCTGAACAGAACTGAGGCTGCTAATGTTGAAAAGATTGTCACAACATTCTTGAGGAGTGGTGTAGTCCCTAGTCAGATTGGAGTGATCACACCATATGAGGGACAGAGAGCTTATATTGTGAACTATATGTCGCGAAATGGTGCTCTCAGACAGCAACTTTATAAGGAAATTGAGGTTGCAAGCGTAGATTCATTTCAGGGAAGGGAAAAGGATTATATCATCCTTTCGTGTGTGAGGAGTAATGAGCATCAGGGCATCGGATTCCTTAATGATCCTCGCAGGCTCAATGTTGCTCTAACACGTGCTCGATATGGTATTGTCATTCTTGGAAACCCTAAAGTTCTTAGTAAACAGCCACTGTGGAATAGCTTGTTGACACATTACAAGGAACACGAGTGCTTGGTTGAGGGGCCTCTAAATAACCTGAAGCAGAGTATGGTGCAATTTCCAAAGCCTAAAAAGATCTACAATGATCGGCGCCTATTCTTTGGAGGTGGTCCTGGAGTTATCCCCAATGACAGTTTTGGGTCTGTAGCCTCAGCTGGCCAAAATGTGGACAGAAGAAGTAATCGTGGTAGGGGTTCTTACCTACCTCCTGGCCCACCCAATGGTACCCATAAGCCTGGTGTACATCCGGCTGGATATCCAATGCCTCGGGCACCTCTTTCACCATTCCACGGAGGACCCCTTTCTCAACCGTATGCTATTCCAACTCGTGGAGCTGTGCATGGACCAGTTGGAGCTGTTCCTCATGTCCCTCAACCTGGAAGTCGTGGTTTTGGGGCAGGACGTGGAAATGCTGGTGCTCCAATTGGCAGTCATCTTCCGCACCAGCAGGGCACACAACAGAATGTTGGGAATATGGGGTCTACCTTCAACTTTCCCGCTCTTGAAAATCCAAATAGCCAACCATCTGTTGGTGGCCCCTTATCTCAACCTGGATTTGTCAATAATATGCCAGTTCAAGGGCCAAGCCAAACATTTCGTGATGGATTTTCCATGGCGGGCATGTCTCAGGAATTTTTGGGTGACGACTTTAAAAGTCAGGGATCACATGTTCCTTATAATGTTGCTGACTTCTCCACTCAGGCTTCTCAAAGTGGATATGCTGTTGATTATGTTACTCAAGGAGCACAAGGTGGGTTTCCAGGGAACTTTCTGAACCAGAACTCTCAAGCTGGATATTCTCGATTTGGTACAGGGAATGACTTCATGTCTCAGGACTACATGCCTCATGGATCACAAGGCCTATTTACTCAGGTGGGCTTTAATGATCCATCACAGGATGAAGCATCACAGAACCACTATGGAGTTGCAAATGCCAATCAACTCCAGTCCCAGGGCTTTATGAACTCGCTGTACTCTCAGCCTTTTGCCCATTACAACACACAGCCAATGAACTTGCAGGCCCCACCACAACAGCAGCAGCAGCAGCAGCAGCAGCCGCAGCCTCAGCCTCAGCCTCAGCAGGGCCAGACCTCCCAAAATCAGAAAATCCACTACAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

1286

Amino Acids

141.05

Weight (kDa)

6.29

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 136 - 289 9e-73 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 341 - 430 4.8e-27 RNA helicase UPF1, 1B domain
AAA_11 PF13086 486 - 607 2.2e-23 AAA domain
AAA_30 PF13604 486 - 687 2.3e-13 AAA domain
ResIII PF04851 486 - 556 4.9e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 493 - 687 6.6e-11 AAA domain
AAA_11 PF13086 588 - 689 3.1e-28 AAA domain
AAA_12 PF13087 698 - 894 1e-61 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2508, 3411
AarI CACCTGC 1 cut(s) 266
Acc36I ACCTGC 4 cut(s) 184, 266, 2011, 2140
Acc65I GGTACC 1 cut(s) 2957
AccB1I GGYRCC 3 cut(s) 2823, 2957, 3004
AccB7I CCANNNNNTGG 5 cut(s) 925, 2899, 2954, 3062, 3263
AccBSI CCGCTC 1 cut(s) 3233
AccI GTMKAC 4 cut(s) 1629, 1967, 1976, 3215
AccII CGCG 2 cut(s) 120, 2479
AccIII TCCGGA 1 cut(s) 1453
AciI CCGC 9 cut(s) 118, 195, 423, 1138, 1852, 3173, 3231, 3350, 3791
AclWI GGATC 7 cut(s) 732, 1510, 1913, 2612, 3403, 3592, 3614
AcoI YGGCCR 3 cut(s) 1929, 2270, 2890
AcsI RAATTY 3 cut(s) 77, 2183, 3365
AcuI CTGAAG 5 cut(s) 141, 1258, 1481, 1868, 2792
AcvI CACGTG 1 cut(s) 2648
AcyI GRCGYC 3 cut(s) 35, 60, 2824
AdeI CACNNNGTG 1 cut(s) 3485
AflII CTTAAG 1 cut(s) 691
AflIII ACRYGT 6 cut(s) 501, 1189, 1755, 1901, 2645, 3400
AhdI GACNNNNNGTC 3 cut(s) 34, 220, 3559
AjiI CACGTC 3 cut(s) 504, 1758, 3137
AjuI GAANNNNNNNTTGG 2 cut(s) 1925, 1957
AloI GAACNNNNNNTCC 2 cut(s) 3387, 3419
Alw21I GWGCWC 8 cut(s) 829, 1444, 1461, 1612, 2491, 2653, 3154, 3481
Alw26I GTCTC 4 cut(s) 1343, 2106, 3363, 3567
Alw44I GTGCAC 1 cut(s) 1440
AlwI GGATC 7 cut(s) 732, 1510, 1913, 2612, 3403, 3592, 3614
AlwNI CAGNNNCTG 1 cut(s) 2704
Ama87I CYCGRG 2 cut(s) 250, 3000
Aor13HI TCCGGA 1 cut(s) 1453
ApaLI GTGCAC 1 cut(s) 1440
ApoI RAATTY 3 cut(s) 77, 2183, 3365
ArsI GACNNNNNNTTYG 2 cut(s) 1020, 1052
AseI ATTAAT 1 cut(s) 837
Asp700I GAANNNNTTC 1 cut(s) 3554
Asp718I GGTACC 1 cut(s) 2957
AspLEI GCGC 2 cut(s) 260, 2826
AsuC2I CCSGG 4 cut(s) 110, 251, 252, 437
AsuHPI GGTGA 4 cut(s) 518, 1139, 3006, 3386
AvaI CYCGRG 2 cut(s) 250, 3000
AvaII GGWCC 9 cut(s) 214, 532, 784, 1411, 1523, 2054, 2843, 3029, 3077
BaeGI GKGCMC 5 cut(s) 814, 1444, 2213, 3007, 3188
BalI TGGCCA 2 cut(s) 1931, 2892
BanI GGYRCC 3 cut(s) 2823, 2957, 3004
BanII GRGCYC 2 cut(s) 15, 1461
BarI GAAGNNNNNNTAC 2 cut(s) 448, 480
BauI CACGAG 3 cut(s) 2082, 2738, 3060
BbrPI CACGTG 1 cut(s) 2648
BbsI GAAGAC 3 cut(s) 551, 1107, 1442
Bbv12I GWGCWC 8 cut(s) 829, 1444, 1461, 1612, 2491, 2653, 3154, 3481
BbvCI CCTCAGC 4 cut(s) 2884, 3798, 3804, 3810
BccI CCATC 7 cut(s) 1383, 2197, 2240, 2302, 3265, 3329, 3631
BceAI ACGGC 3 cut(s) 48, 334, 342
BcgI CGANNNNNNTGC 2 cut(s) 264, 298
BciVI GTATCC 1 cut(s) 349
BclI TGATCA 1 cut(s) 2430
BcnI CCSGG 4 cut(s) 110, 251, 252, 437
BcoDI GTCTC 4 cut(s) 1343, 2106, 3363, 3567
BfaI CTAG 6 cut(s) 270, 1614, 1637, 1818, 2331, 2415
BfmI CTRYAG 4 cut(s) 177, 1554, 1626, 2877
BfoI RGCGCY 2 cut(s) 261, 2827
BfrI CTTAAG 1 cut(s) 691
BfuAI ACCTGC 4 cut(s) 184, 266, 2011, 2140
BfuI GTATCC 1 cut(s) 349
BglI GCCNNNNNGGC 1 cut(s) 266
BglII AGATCT 2 cut(s) 886, 2808
Bme18I GGWCC 9 cut(s) 214, 532, 784, 1411, 1523, 2054, 2843, 3029, 3077
BmeRI GACNNNNNGTC 3 cut(s) 34, 220, 3559
BmeT110I CYCGRG 2 cut(s) 250, 3000
BmgBI CACGTC 3 cut(s) 504, 1758, 3137
BmrI ACTGGG 4 cut(s) 369, 730, 1668, 2092
BmuI ACTGGG 4 cut(s) 369, 730, 1668, 2092
BpiI GAAGAC 3 cut(s) 551, 1107, 1442
BpmI CTGGAG 4 cut(s) 1173, 1935, 2868, 3662
Bpu10I CCTNAGC 5 cut(s) 806, 2884, 3798, 3804, 3810
BpuEI CTTGAG 5 cut(s) 911, 1455, 2416, 3456, 3504
BpuMI CCSGG 4 cut(s) 110, 251, 252, 437
BsaAI YACGTR 1 cut(s) 2648
BsaHI GRCGYC 3 cut(s) 35, 60, 2824
BsaWI WCCGGW 1 cut(s) 1453
BsaXI ACNNNNNCTCC 2 cut(s) 1239, 1269
Bse118I RCCGGY 4 cut(s) 235, 296, 414, 2272
Bse3DI GCAATG 1 cut(s) 1421
BseAI TCCGGA 1 cut(s) 1453
BseMI GCAATG 1 cut(s) 1421
BseRI GAGGAG 4 cut(s) 1229, 2333, 2413, 2596
BseSI GKGCMC 5 cut(s) 814, 1444, 2213, 3007, 3188
BseYI CCCAGC 2 cut(s) 329, 2087
BsgI GTGCAG 1 cut(s) 1191
Bsh1236I CGCG 2 cut(s) 120, 2479
BshNI GGYRCC 3 cut(s) 2823, 2957, 3004
BsiHKAI GWGCWC 8 cut(s) 829, 1444, 1461, 1612, 2491, 2653, 3154, 3481
BsiHKCI CYCGRG 2 cut(s) 250, 3000
BslFI GGGAC 9 cut(s) 726, 770, 1200, 1605, 2271, 2396, 2460, 3086, 3667
BsmAI GTCTC 4 cut(s) 1343, 2106, 3363, 3567
BsmFI GGGAC 9 cut(s) 726, 770, 1200, 1605, 2271, 2396, 2460, 3086, 3667
BsmI GAATGC 3 cut(s) 602, 1994, 2169
BsoBI CYCGRG 2 cut(s) 250, 3000
Bsp13I TCCGGA 1 cut(s) 1453
Bsp1407I TGTACA 3 cut(s) 1776, 2302, 2974
Bsp19I CCATGG 2 cut(s) 1267, 3345
Bsp68I TCGCGA 1 cut(s) 2479
BspACI CCGC 9 cut(s) 118, 195, 423, 1138, 1852, 3173, 3231, 3350, 3791
BspEI TCCGGA 1 cut(s) 1453
BspFNI CGCG 2 cut(s) 120, 2479
BspMAI CTGCAG 2 cut(s) 181, 1558
BspMI ACCTGC 4 cut(s) 184, 266, 2011, 2140
BspPI GGATC 7 cut(s) 732, 1510, 1913, 2612, 3403, 3592, 3614
BspT107I GGYRCC 3 cut(s) 2823, 2957, 3004
BspTI CTTAAG 1 cut(s) 691
BsrBI CCGCTC 1 cut(s) 3233
BsrDI GCAATG 1 cut(s) 1421
BsrFI RCCGGY 4 cut(s) 235, 296, 414, 2272
BsrGI TGTACA 3 cut(s) 1776, 2302, 2974
BssAI RCCGGY 4 cut(s) 235, 296, 414, 2272
BssNI GRCGYC 3 cut(s) 35, 60, 2824
BssSI CACGAG 3 cut(s) 2082, 2738, 3060
BssT1I CCWWGG 3 cut(s) 1267, 2267, 3345
Bst2BI CACGAG 3 cut(s) 2082, 2738, 3060
Bst4CI ACNGT 8 cut(s) 185, 191, 608, 1546, 1776, 2706, 2867, 3045
Bst6I CTCTTC 1 cut(s) 1289
BstACI GRCGYC 3 cut(s) 35, 60, 2824
BstAFI CTTAAG 1 cut(s) 691
BstAPI GCANNNNNTGC 1 cut(s) 2084
BstAUI TGTACA 3 cut(s) 1776, 2302, 2974
BstBAI YACGTR 1 cut(s) 2648
BstDSI CCRYGG 4 cut(s) 190, 1267, 3022, 3345
BstENI CCTNNNNNAGG 1 cut(s) 1533
BstFNI CGCG 2 cut(s) 120, 2479
BstH2I RGCGCY 2 cut(s) 261, 2827
BstHHI GCGC 2 cut(s) 260, 2826
BstMAI GTCTC 4 cut(s) 1343, 2106, 3363, 3567
BstNSI RCATGY 6 cut(s) 410, 1193, 1905, 3358, 3404, 3577
BstSFI CTRYAG 4 cut(s) 177, 1554, 1626, 2877
BstSLI GKGCMC 5 cut(s) 814, 1444, 2213, 3007, 3188
BstUI CGCG 2 cut(s) 120, 2479
BstV2I GAAGAC 3 cut(s) 551, 1107, 1442
BstX2I RGATCY 4 cut(s) 724, 886, 1918, 2808
BstXI CCANNNNNNTGG 2 cut(s) 2969, 3851
BstYI RGATCY 4 cut(s) 724, 886, 1918, 2808
BsuI GTATCC 1 cut(s) 349
BtgI CCRYGG 4 cut(s) 190, 1267, 3022, 3345
BtgZI GCGATG 1 cut(s) 1839
BtrI CACGTC 3 cut(s) 504, 1758, 3137
BtsI GCAGTG 3 cut(s) 776, 1347, 2041
BtsIMutI CAGTG 9 cut(s) 740, 776, 829, 1188, 1282, 1347, 1844, 2041, 2702
BtuMI TCGCGA 1 cut(s) 2479
BveI ACCTGC 4 cut(s) 184, 266, 2011, 2140
CaiI CAGNNNCTG 1 cut(s) 2704
CfoI GCGC 2 cut(s) 260, 2826
Cfr10I RCCGGY 4 cut(s) 235, 296, 414, 2272
Cfr9I CCCGGG 1 cut(s) 250
CseI GACGC 3 cut(s) 24, 68, 219
CsiI ACCWGGT 4 cut(s) 528, 1123, 1444, 1737
DinI GGCGCC 1 cut(s) 2825
DraI TTTAAA 1 cut(s) 3385
DraIII CACNNNGTG 1 cut(s) 3485
DriI GACNNNNNGTC 3 cut(s) 34, 220, 3559
EaeI YGGCCR 3 cut(s) 1929, 2270, 2890
Eam1104I CTCTTC 1 cut(s) 1289
Eam1105I GACNNNNNGTC 3 cut(s) 34, 220, 3559
EarI CTCTTC 1 cut(s) 1289
Ecl136II GAGCTC 1 cut(s) 1459
Eco130I CCWWGG 3 cut(s) 1267, 2267, 3345
Eco147I AGGCCT 1 cut(s) 3594
Eco24I GRGCYC 2 cut(s) 15, 1461
Eco32I GATATC 1 cut(s) 2990
Eco47I GGWCC 9 cut(s) 214, 532, 784, 1411, 1523, 2054, 2843, 3029, 3077
Eco53kI GAGCTC 1 cut(s) 1459
Eco57I CTGAAG 5 cut(s) 141, 1258, 1481, 1868, 2792
Eco72I CACGTG 1 cut(s) 2648
Eco88I CYCGRG 2 cut(s) 250, 3000
EcoICRI GAGCTC 1 cut(s) 1459
EcoNI CCTNNNNNAGG 1 cut(s) 1533
EcoO109I RGGNCCY 4 cut(s) 784, 2756, 3029, 3754
EcoRI GAATTC 1 cut(s) 77
EcoRV GATATC 1 cut(s) 2990
EcoT14I CCWWGG 3 cut(s) 1267, 2267, 3345
EcoT22I ATGCAT 3 cut(s) 706, 1468, 2169
EcoT38I GRGCYC 2 cut(s) 15, 1461
EgeI GGCGCC 1 cut(s) 2825
EheI GGCGCC 1 cut(s) 2825
ErhI CCWWGG 3 cut(s) 1267, 2267, 3345
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FaqI GGGAC 9 cut(s) 726, 770, 1200, 1605, 2271, 2396, 2460, 3086, 3667
FauI CCCGC 3 cut(s) 1845, 3238, 3343
FauNDI CATATG 1 cut(s) 2440
FbaI TGATCA 1 cut(s) 2430
FblI GTMKAC 4 cut(s) 1629, 1967, 1976, 3215
FriOI GRGCYC 2 cut(s) 15, 1461
FspBI CTAG 6 cut(s) 270, 1614, 1637, 1818, 2331, 2415
GlaI GCGC 2 cut(s) 259, 2825
GsaI CCCAGC 2 cut(s) 333, 2091
GsuI CTGGAG 4 cut(s) 1173, 1935, 2868, 3662
HaeII RGCGCY 2 cut(s) 261, 2827
HgaI GACGC 3 cut(s) 24, 68, 219
HhaI GCGC 2 cut(s) 260, 2826
Hin1I GRCGYC 3 cut(s) 35, 60, 2824
Hin6I GCGC 2 cut(s) 258, 2824
HinP1I GCGC 2 cut(s) 258, 2824
HincII GTYRAC 4 cut(s) 316, 1203, 1282, 2721
HindII GTYRAC 4 cut(s) 316, 1203, 1282, 2721
HindIII AAGCTT 1 cut(s) 1332
HinfI GANTC 9 cut(s) 5, 127, 161, 523, 1016, 1964, 1996, 2534, 2607
HpaI GTTAAC 1 cut(s) 1203
HphI GGTGA 4 cut(s) 518, 1139, 3006, 3386
Hpy99I CGWCG 4 cut(s) 40, 62, 235, 1434
HpyCH4III ACNGT 8 cut(s) 185, 191, 608, 1546, 1776, 2706, 2867, 3045
HpyCH4IV ACGT 5 cut(s) 503, 1114, 1757, 2647, 3136
HpySE526I ACGT 5 cut(s) 503, 1114, 1757, 2647, 3136
Hsp92I GRCGYC 3 cut(s) 35, 60, 2824
HspAI GCGC 2 cut(s) 258, 2824
KasI GGCGCC 1 cut(s) 2823
Kpn2I TCCGGA 1 cut(s) 1453
KpnI GGTACC 1 cut(s) 2961
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 2430
KspAI GTTAAC 1 cut(s) 1203
LmnI GCTCC 8 cut(s) 799, 1456, 1615, 1981, 3065, 3086, 3157, 3476
MabI ACCWGGT 4 cut(s) 528, 1123, 1444, 1737
MaeI CTAG 6 cut(s) 270, 1614, 1637, 1818, 2331, 2415
MaeII ACGT 5 cut(s) 503, 1114, 1757, 2647, 3136
MaeIII GTNAC 9 cut(s) 185, 290, 1036, 1110, 1546, 2227, 2383, 3374, 3466
MbiI CCGCTC 1 cut(s) 3233
MfeI CAATTG 3 cut(s) 1560, 1790, 3156
MflI RGATCY 4 cut(s) 724, 886, 1918, 2808
MlsI TGGCCA 2 cut(s) 1931, 2892
MluNI TGGCCA 2 cut(s) 1931, 2892
Mly113I GGCGCC 1 cut(s) 2824
MlyI GAGTC 1 cut(s) 1973
MmeI TCCRAC 5 cut(s) 78, 192, 2014, 3064, 3080
Mox20I TGGCCA 2 cut(s) 1931, 2892
Mph1103I ATGCAT 3 cut(s) 706, 1468, 2169
MroI TCCGGA 1 cut(s) 1453
MroNI GCCGGC 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 3554
MscI TGGCCA 2 cut(s) 1931, 2892
MslI CAYNNNNRTG 1 cut(s) 3849
Msp20I TGGCCA 2 cut(s) 1931, 2892
MspA1I CMGCKG 1 cut(s) 2888
MspCI CTTAAG 1 cut(s) 691
MunI CAATTG 3 cut(s) 1560, 1790, 3156
Mva1269I GAATGC 3 cut(s) 602, 1994, 2169
MvnI CGCG 2 cut(s) 120, 2479
NaeI GCCGGC 1 cut(s) 298
NarI GGCGCC 1 cut(s) 2824
NciI CCSGG 4 cut(s) 110, 251, 252, 437
NcoI CCATGG 2 cut(s) 1267, 3345
NdeI CATATG 1 cut(s) 2440
NgoMIV GCCGGC 1 cut(s) 296
NmuCI GTSAC 6 cut(s) 185, 1036, 1546, 2227, 2383, 3374
NruI TCGCGA 1 cut(s) 2479
NsiI ATGCAT 3 cut(s) 706, 1468, 2169
NspI RCATGY 6 cut(s) 410, 1193, 1905, 3358, 3404, 3577
PaqCI CACCTGC 1 cut(s) 266
PasI CCCWGGG 2 cut(s) 88, 3685
PceI AGGCCT 1 cut(s) 3594
PciI ACATGT 3 cut(s) 1189, 1901, 3400
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PctI GAATGC 3 cut(s) 602, 1994, 2169
PdiI GCCGGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 3554
PfeI GAWTC 8 cut(s) 5, 127, 161, 523, 1016, 1996, 2534, 2607
PflFI GACNNNGTC 2 cut(s) 1034, 1286
PflMI CCANNNNNTGG 5 cut(s) 925, 2899, 2954, 3062, 3263
PfoI TCCNGGA 1 cut(s) 2845
PleI GAGTC 1 cut(s) 1972
PluTI GGCGCC 1 cut(s) 2827
PmaCI CACGTG 1 cut(s) 2648
PmlI CACGTG 1 cut(s) 2648
PpsI GAGTC 1 cut(s) 1972
Ppu21I YACGTR 1 cut(s) 2648
PpuMI RGGWCCY 2 cut(s) 784, 3029
PscI ACATGT 3 cut(s) 1189, 1901, 3400
PshBI ATTAAT 1 cut(s) 837
PsiI TTATAA 2 cut(s) 2508, 3411
Psp124BI GAGCTC 1 cut(s) 1461
Psp5II RGGWCCY 2 cut(s) 784, 3029
PspCI CACGTG 1 cut(s) 2648
PspFI CCCAGC 2 cut(s) 329, 2087
PspPPI RGGWCCY 2 cut(s) 784, 3029
PsrI GAACNNNNNNTAC 2 cut(s) 3689, 3721
PstI CTGCAG 2 cut(s) 181, 1558
PstNI CAGNNNCTG 1 cut(s) 2704
PsuI RGATCY 4 cut(s) 724, 886, 1918, 2808
PsyI GACNNNGTC 2 cut(s) 1034, 1286
PvuII CAGCTG 1 cut(s) 2888
RruI TCGCGA 1 cut(s) 2479
RseI CAYNNNNRTG 1 cut(s) 3849
SacI GAGCTC 1 cut(s) 1461
SchI GAGTC 1 cut(s) 1973
SexAI ACCWGGT 4 cut(s) 528, 1123, 1444, 1737
SfcI CTRYAG 4 cut(s) 177, 1554, 1626, 2877
SfoI GGCGCC 1 cut(s) 2825
SinI GGWCC 9 cut(s) 214, 532, 784, 1411, 1523, 2054, 2843, 3029, 3077
SmaI CCCGGG 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 3849
SmlI CTYRAG 6 cut(s) 691, 890, 1470, 2395, 3471, 3519
SmoI CTYRAG 6 cut(s) 691, 890, 1470, 2395, 3471, 3519
SseBI AGGCCT 1 cut(s) 3594
SsiI CCGC 9 cut(s) 118, 195, 423, 1138, 1852, 3173, 3231, 3350, 3791
SspDI GGCGCC 1 cut(s) 2823
SspMI CTAG 6 cut(s) 270, 1614, 1637, 1818, 2331, 2415
SstI GAGCTC 1 cut(s) 1461
StuI AGGCCT 1 cut(s) 3594
StyI CCWWGG 3 cut(s) 1267, 2267, 3345
TaaI ACNGT 8 cut(s) 185, 191, 608, 1546, 1776, 2706, 2867, 3045
TaiI ACGT 5 cut(s) 506, 1117, 1760, 2650, 3139
TaqI TCGA 5 cut(s) 75, 431, 472, 2653, 3535
TatI WGTACW 5 cut(s) 1298, 1776, 2302, 2974, 3705
TauI GCSGC 3 cut(s) 426, 1141, 3793
TfiI GAWTC 8 cut(s) 5, 127, 161, 523, 1016, 1996, 2534, 2607
TscAI CASTG 9 cut(s) 740, 776, 829, 1188, 1282, 1347, 1851, 2048, 2709
TseFI GTSAC 6 cut(s) 185, 1036, 1546, 2227, 2383, 3374
Tsp45I GTSAC 6 cut(s) 185, 1036, 1546, 2227, 2383, 3374
TspGWI ACGGA 4 cut(s) 1403, 1934, 1994, 3039
TspMI CCCGGG 1 cut(s) 250
TspRI CASTG 9 cut(s) 740, 776, 829, 1188, 1282, 1347, 1851, 2048, 2709
Tth111I GACNNNGTC 2 cut(s) 1034, 1286
Van91I CCANNNNNTGG 5 cut(s) 925, 2899, 2954, 3062, 3263
Vha464I CTTAAG 1 cut(s) 691
VneI GTGCAC 1 cut(s) 1440
VpaK11BI GGWCC 9 cut(s) 214, 532, 784, 1411, 1523, 2054, 2843, 3029, 3077
VspI ATTAAT 1 cut(s) 837
XagI CCTNNNNNAGG 1 cut(s) 1533
XapI RAATTY 3 cut(s) 77, 2183, 3365
XceI RCATGY 6 cut(s) 410, 1193, 1905, 3358, 3404, 3577
XcmI CCANNNNNNNNNTGG 2 cut(s) 1526, 3260
XmaI CCCGGG 1 cut(s) 250
XmiI GTMKAC 4 cut(s) 1629, 1967, 1976, 3215
XmnI GAANNNNTTC 1 cut(s) 3554
XspI CTAG 6 cut(s) 270, 1614, 1637, 1818, 2331, 2415
Zsp2I ATGCAT 3 cut(s) 706, 1468, 2169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.