Rh7CG249400

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0009459
Physical Location & Seq
Reverse (-)
23497839 .. 23508659
10821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG249400.1

Sequence Viewer

Length: 3843 bp
ATGGAATCGCAGCCGAACAACCTCTTCGACACGGCGTCGCAGCCGGACACGGCCACCGACGCCTACACTTTTCTCGAATTCAACACCCAGGGCGAGGACTTCGACTACCCGGAGTTTCGCGACCCGATTCGGTCCCCGGTGGCCTGGCCCACGCCGTCCGATTCCTTATCGGACCCTGCCGACCGCGACCGCGACCGCGACCGCGGCGGAGGAGGTGGTGGTGGTGGCTCCGACCACCAGTCTGATGCGTCGCCTGTTTCGGCTGCCCCGGGCAGCGCCACGAAGGCCCGGGCCGGTGGCTCGGGCAGTAGCGGCGGGAATAATAATCAGGTGGTTGACGCCCTAGCGTCTGGGATGAGTGTGCTGAACTTTGAGGACACTGGGGATGATGATAGCTATGAGTTTGGAAAGGGGGATTTTACTGAGCATGCGTGCCGGTACTGCGGTGTGTCGAACCCGGCGTGCGTTGTGAGGTGTAACGTGCCGTCGTGCCGGAAATGGTTTTGTAATTCCAGGGGGAACACGTCCGGTTCTCATATTGTGAATCACCTGGTTCGTGCAAAACACAAGGAAGTCTGTCTTCATAAAGATAGCCCATTAGGAGAAACAATCCTTGAGTGCTACAACTGTGGATGCCGCAATGTATTCCTTCTTGGATTTATTTCAGCCAAGACAGAGAGTGTTGTCGTTCTTCTCTGTAGGGAACCCTGCTTAAGTGTTAATGCGTTGAAGGATATGAACTGGGACCTGAGCCAGTGGTGTCCTCTGATTGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGTCCCCTCCGAACAGGAGCAGCTGAGGGCACGCCAGATTAGTGCTCAACAAATAAACAAGGTAGAAGAACTCTGGAAGTCAAATCCTGATGCTTCCCTTGAAGATCTTGAGAAGCCTGGTGTGGATGATGAACCCCAACCTGTGGCCGTAAAGTATGAGGATGCATACCAGTATCAAAATGTGTTTGCACCACTTATCAAGCTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGCAAGGACAATCTCACCATTAGGTGGGATATTGGTCTTAACAAGAAGCGTGTCGCTTATTTTATTTTTCCAAAGGAGGACAATGAGATTCGTCTTGTACCTGGTGATGAGCTGCGGCTTCGTTATTCTGGGGATGCAGCTCATCCAGCATGGCAGTCTGTGGGACATGTGATCAAGTTAACTGCACAAGAGGAGGTTGCGCTCGAACTTCGTGCTAGTCAGGGAGTTCCAGCTGATGTGAACCATGGCTTCAGCGTTGATTTCGTGTGGAAAAGTACAAGTTTTGATCGTATGCAGGGAGCCATGAAAGCTTTTGCAGTGGATGAGACAAGTGTTAGTGGGTATATTTACCATCACTTGTTAGGCCATGAAGTAGAGGTTCAGATGGTCCGTAATACACTGCCTCGTCGTTTTGGTGCACCTGGTCTGCCAGAACTCAATGCATCTCAGGTTTTTGCTGTGAAAAGTGTCCTTCAGAAGCCTATAAGTTTGATACAAGGTCCACCTGGCACAGGAAAAACTGTCACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGGCAGGGACAGGTCTTGGTTTGCGCTCCTAGTAATGTGGCTGTAGACCAACTAGCTGAAAAAATAAGTGCAACTGGGCTGAAGGTTGTTAGACTTTGTGCAAAATCAAGGGAAGCTGTCAGCTCTCCTGTTGAGCATTTAACCCTTCACTATCAGGTTCGTCATCTTGACACATCAGAAAAGAGTGAACTTCACAAGTTACAGCAATTGAAGGATGAGCAAGGCGAATTGTCAAGTAGTGATGAGAAAAAGTACAAAGCACTGAAGCGGGCAACTGAGAGAGAGATTTCTCAGAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGAGATCCTCGATTGGCGAATTTTAGGTTCCGTCAGGTACTTATTGATGAGTCTACTCAGGCAACAGAGCCTGAATGCCTTATTCCTTTGGTACTAGGGGCAAAGCAGGTTGTTTTGGTTGGTGATCACTGCCAACTTGGTCCAGTCATTATGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTTTTTGAACGCCTCGTTTTACTTGGTGTGAAACCAATTAGGTTGCAGGTCCAATACAGAATGCATCCAGCCTTGTCAGAATTTCCTTCCAACAGCTTCTATGAGGGAACACTCCAAAATGGCGTCACCATCAATGAAAGGCAATCAACGGGAATTGATTTTCCATGGCCAGTTCCCAATCGTCCCATGTTCTTTTATGTTCAGATGGGGCAAGAGGAGATAAGTGCTAGCGGAACATCTTACCTGAACAGAACTGAAGCTGCTAATGTTGAAAAGATTGTCACAACATTCTTAAGGAGTGGTGTAGTCCCTAGTCAGATCGGGGTGATAACACCATATGAGGGACAGCGAGCATATATTGTGAACTATATGTCGAGAAATGGTGCTCTCAGGCAGCAACTTTATAAGGAAATAGAGGTTGCAAGTGTAGATTCATTTCAAGGAAGGGAAAAAGATTATATCATCCTTTCATGTGTGAGGAGTAATGAGCATCAGGGCATTGGATTCCTTAATGACCCTCGGAGGCTCAATGTTGCCCTAACACGTGCTCGGTATGGTATTGTTATTCTGGGGAACCCTAAAGTTCTAAGTAAACAGCCCTTGTGGAATAGCTTGTTGACGCACTACAAGGAACATGAGTGCTTGGTCGAAGGTCCTCTAAATAACTTGAAGCAGAGTATGGTTCAATTTCCAAAGCCCAAAAAGATCTACAATGATCGGCGTCTGTTCTTTGGTGGTGGTCCTGGAGTTGTCCCAAGTGATAGTTATGGGTCTATTCCCCCATCCGGTCAGAGTGCTGATAGAAGAAGTGGTCGTGGTAGGGGCTCTTACCTACCCCCTGGTCCACCGAATGGTACCCACAAGCCTGGGGTACATCCTGCTGGATATCCAATGCCTCGGGCACCACTTGCACCATTTCATGGTGGTCCCCTTTCTCAACCCTATGCTATTCCAACTCGTGGAGCCGTGCATGGACCAGTAGGAGCTGTTCCTCATGTCCCTCAACCTGGAAGTCGAGGTTTTGGAGCTGGACGTGGCAGTGCTGGTGCTCCTATTGGCAGTCATCTCCAACACCAGCAAGGCACACAGCAGAATGTAGGAAATCTGGGGTCTAACTTCAACTTTCCTGCTCTGGAAAATCCTAATAGTCAGCCATCTGTGGGCGGTCCATTATCTCAACCTGGATTTGTCAATAATATGCCAGTTCAAGGGCCAAGCCAAACATTTCGTGATGGATTTTCCATGGCGGGCATGTCTCAGGAGTTCTTGGGTGATGACTTTAAAAGTCAGGGATCTCATGTTCCTTATAACGTTGCTGACTTTTCCACTCAGGCTTCTCAAAGTGGATATGCTGTTGATTATGTTACACAAGGAGCACAAGGAGGATTTCCAGGCAATTTTATAAACCAGAATTCTCAAGCTGGGTACTCTCGATTTGGTACAGGGAATGACTTCATGTCTCAGGACTACATGGCTCATGGTACACAAGGTCTATTTACCCAGGTGGGCTACAATGACCCATCACAGGATGATGGATCGCAGAACCACTATGGTGTGGGGAATGCTAATGCACTTCAGTCTCAGGGATATATGAATTCTCTGTACTCCCAACCTTTTACCCACTACAACACACAGCCAATGAACTTGCAGGCCCCACAACAGCAGCCGCCTCAGCAGGGTCAGAGCTCCCAAAATCAGAAGATTCACTACAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

1280

Amino Acids

140.03

Weight (kDa)

6.35

Isoelectric Point (pI)

49.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 143 - 296 9e-73 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 348 - 437 1.8e-26 RNA helicase UPF1, 1B domain
AAA_11 PF13086 493 - 613 2.1e-23 AAA domain
AAA_30 PF13604 493 - 694 6e-14 AAA domain
ResIII PF04851 493 - 563 4.9e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 500 - 694 3.5e-11 AAA domain
AAA_11 PF13086 595 - 696 1e-28 AAA domain
AAA_12 PF13087 705 - 901 1.2e-61 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2529, 3432, 3527
Acc36I ACCTGC 2 cut(s) 2032, 2161
Acc65I GGTACC 1 cut(s) 2978
AccB1I GGYRCC 2 cut(s) 2978, 3025
AccB7I CCANNNNNTGG 5 cut(s) 946, 1068, 2975, 3044, 3083
AccI GTMKAC 2 cut(s) 1650, 1988
AccII CGCG 5 cut(s) 120, 186, 192, 198, 204
AclI AACGTT 1 cut(s) 3435
AclWI GGATC 3 cut(s) 1934, 3424, 3667
AcoI YGGCCR 3 cut(s) 51, 948, 2291
AcsI RAATTY 5 cut(s) 77, 1954, 2204, 3535, 3718
AcuI CTGAAG 6 cut(s) 1279, 1502, 1706, 1889, 2400, 3683
AcvI CACGTG 1 cut(s) 2669
AcyI GRCGYC 5 cut(s) 35, 60, 339, 2247, 2845
AflII CTTAAG 2 cut(s) 712, 2416
AflIII ACRYGT 4 cut(s) 522, 1210, 1922, 2666
AhdI GACNNNNNGTC 3 cut(s) 34, 238, 3580
AjiI CACGTC 2 cut(s) 525, 3158
AleI CACNNNNGTG 1 cut(s) 3675
AloI GAACNNNNNNTCC 2 cut(s) 3408, 3440
Alw21I GWGCWC 7 cut(s) 850, 1465, 2512, 2674, 3175, 3502, 3812
Alw26I GTCTC 5 cut(s) 1364, 2127, 3384, 3588, 3708
Alw44I GTGCAC 1 cut(s) 1461
AlwI GGATC 3 cut(s) 1934, 3424, 3667
AlwNI CAGNNNCTG 1 cut(s) 2006
Ama87I CYCGRG 4 cut(s) 268, 288, 301, 3021
ApaLI GTGCAC 1 cut(s) 1461
ApoI RAATTY 5 cut(s) 77, 1954, 2204, 3535, 3718
Asp700I GAANNNNTTC 1 cut(s) 3575
Asp718I GGTACC 1 cut(s) 2978
AspLEI GCGC 3 cut(s) 278, 1246, 1631
AsuC2I CCSGG 7 cut(s) 110, 137, 269, 270, 289, 290, 458
AsuHPI GGTGA 7 cut(s) 539, 1051, 1160, 2069, 2242, 2461, 3407
AsuNHI GCTAGC 1 cut(s) 2351
AvaI CYCGRG 4 cut(s) 268, 288, 301, 3021
BaeGI GKGCMC 3 cut(s) 835, 1465, 3028
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BalI TGGCCA 1 cut(s) 2293
BanI GGYRCC 2 cut(s) 2978, 3025
BanII GRGCYC 2 cut(s) 2951, 3812
BarI GAAGNNNNNNTAC 1 cut(s) 3815
BauI CACGAG 2 cut(s) 2103, 3081
BbrPI CACGTG 1 cut(s) 2669
BbsI GAAGAC 1 cut(s) 572
Bbv12I GWGCWC 7 cut(s) 850, 1465, 2512, 2674, 3175, 3502, 3812
BbvCI CCTCAGC 2 cut(s) 827, 3795
BccI CCATC 9 cut(s) 1404, 1423, 2261, 2323, 2914, 3286, 3350, 3650, 3652
BceAI ACGGC 6 cut(s) 48, 66, 139, 469, 935, 3074
BcgI CGANNNNNNTGC 2 cut(s) 1238, 1272
BclI TGATCA 2 cut(s) 1215, 2059
BcnI CCSGG 7 cut(s) 110, 137, 269, 270, 289, 290, 458
BcoDI GTCTC 5 cut(s) 1364, 2127, 3384, 3588, 3708
BfaI CTAG 7 cut(s) 344, 1260, 1635, 1658, 2030, 2352, 2436
BfmI CTRYAG 3 cut(s) 697, 1575, 1647
BfoI RGCGCY 1 cut(s) 279
BfrI CTTAAG 2 cut(s) 712, 2416
BfuAI ACCTGC 2 cut(s) 2032, 2161
BglI GCCNNNNNGGC 1 cut(s) 284
BglII AGATCT 2 cut(s) 907, 2829
BmeRI GACNNNNNGTC 3 cut(s) 34, 238, 3580
BmeT110I CYCGRG 4 cut(s) 268, 288, 301, 3021
BmgBI CACGTC 2 cut(s) 525, 3158
BmrI ACTGGG 4 cut(s) 390, 751, 1689, 2113
BmsI GCATC 8 cut(s) 235, 623, 883, 955, 1168, 1496, 2197, 2623
BmtI GCTAGC 1 cut(s) 2355
BmuI ACTGGG 4 cut(s) 390, 751, 1689, 2113
BpiI GAAGAC 1 cut(s) 572
BpmI CTGGAG 2 cut(s) 1956, 2889
Bpu10I CCTNAGC 3 cut(s) 749, 827, 3795
BpuEI CTTGAG 3 cut(s) 635, 932, 3525
BpuMI CCSGG 7 cut(s) 110, 137, 269, 270, 289, 290, 458
BsaAI YACGTR 1 cut(s) 2669
BsaHI GRCGYC 5 cut(s) 35, 60, 339, 2247, 2845
BsaWI WCCGGW 2 cut(s) 527, 2909
Bse118I RCCGGY 2 cut(s) 293, 435
Bse3DI GCAATG 1 cut(s) 646
BseMI GCAATG 1 cut(s) 646
BseRI GAGGAG 4 cut(s) 225, 1250, 2354, 2617
BseSI GKGCMC 3 cut(s) 835, 1465, 3028
BseYI CCCAGC 2 cut(s) 2108, 3545
BsgI GTGCAG 1 cut(s) 1212
Bsh1236I CGCG 5 cut(s) 120, 186, 192, 198, 204
Bsh1285I CGRYCG 4 cut(s) 184, 190, 196, 202
BshNI GGYRCC 2 cut(s) 2978, 3025
BsiEI CGRYCG 4 cut(s) 184, 190, 196, 202
BsiHKAI GWGCWC 7 cut(s) 850, 1465, 2512, 2674, 3175, 3502, 3812
BsiHKCI CYCGRG 4 cut(s) 268, 288, 301, 3021
BsmAI GTCTC 5 cut(s) 1364, 2127, 3384, 3588, 3708
BsmI GAATGC 3 cut(s) 2015, 2190, 3691
BsoBI CYCGRG 4 cut(s) 268, 288, 301, 3021
Bsp19I CCATGG 3 cut(s) 1288, 2288, 3366
Bsp68I TCGCGA 1 cut(s) 120
BspFNI CGCG 5 cut(s) 120, 186, 192, 198, 204
BspMAI CTGCAG 1 cut(s) 1579
BspMI ACCTGC 2 cut(s) 2032, 2161
BspOI GCTAGC 1 cut(s) 2355
BspPI GGATC 3 cut(s) 1934, 3424, 3667
BspT107I GGYRCC 2 cut(s) 2978, 3025
BspTI CTTAAG 2 cut(s) 712, 2416
BsrDI GCAATG 1 cut(s) 646
BsrFI RCCGGY 2 cut(s) 293, 435
BssAI RCCGGY 2 cut(s) 293, 435
BssNI GRCGYC 5 cut(s) 35, 60, 339, 2247, 2845
BssSI CACGAG 2 cut(s) 2103, 3081
BssT1I CCWWGG 3 cut(s) 1288, 2288, 3366
Bst2BI CACGAG 2 cut(s) 2103, 3081
Bst4CI ACNGT 2 cut(s) 629, 1567
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 5 cut(s) 35, 60, 339, 2247, 2845
BstAFI CTTAAG 2 cut(s) 712, 2416
BstAPI GCANNNNNTGC 2 cut(s) 2105, 3032
BstBAI YACGTR 1 cut(s) 2669
BstDSI CCRYGG 4 cut(s) 202, 1288, 2288, 3366
BstENI CCTNNNNNAGG 1 cut(s) 1554
BstFNI CGCG 5 cut(s) 120, 186, 192, 198, 204
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 3 cut(s) 278, 1246, 1631
BstMAI GTCTC 5 cut(s) 1364, 2127, 3384, 3588, 3708
BstMCI CGRYCG 4 cut(s) 184, 190, 196, 202
BstNSI RCATGY 4 cut(s) 431, 1214, 1926, 3379
BstSFI CTRYAG 3 cut(s) 697, 1575, 1647
BstSLI GKGCMC 3 cut(s) 835, 1465, 3028
BstUI CGCG 5 cut(s) 120, 186, 192, 198, 204
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 4 cut(s) 907, 1939, 2829, 3416
BstXI CCANNNNNNTGG 1 cut(s) 2990
BstYI RGATCY 4 cut(s) 907, 1939, 2829, 3416
BtgI CCRYGG 4 cut(s) 202, 1288, 2288, 3366
BtrI CACGTC 2 cut(s) 525, 3158
BtsI GCAGTG 5 cut(s) 797, 1368, 1442, 2062, 3169
BtsIMutI CAGTG 8 cut(s) 378, 761, 797, 1368, 1442, 1865, 2062, 3169
BtuMI TCGCGA 1 cut(s) 120
BveI ACCTGC 2 cut(s) 2032, 2161
CaiI CAGNNNCTG 1 cut(s) 2006
CfoI GCGC 3 cut(s) 278, 1246, 1631
Cfr10I RCCGGY 2 cut(s) 293, 435
Cfr42I CCGCGG 1 cut(s) 205
Cfr9I CCCGGG 2 cut(s) 268, 288
CseI GACGC 8 cut(s) 24, 68, 237, 336, 347, 2236, 2752, 2834
CsiI ACCWGGT 3 cut(s) 549, 1144, 1465
DraI TTTAAA 1 cut(s) 3406
DriI GACNNNNNGTC 3 cut(s) 34, 238, 3580
EaeI YGGCCR 3 cut(s) 51, 948, 2291
Eam1104I CTCTTC 1 cut(s) 29
Eam1105I GACNNNNNGTC 3 cut(s) 34, 238, 3580
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 222
Ecl136II GAGCTC 1 cut(s) 3810
Eco130I CCWWGG 3 cut(s) 1288, 2288, 3366
Eco24I GRGCYC 2 cut(s) 2951, 3812
Eco32I GATATC 1 cut(s) 3011
Eco53kI GAGCTC 1 cut(s) 3810
Eco57I CTGAAG 6 cut(s) 1279, 1502, 1706, 1889, 2400, 3683
Eco72I CACGTG 1 cut(s) 2669
Eco88I CYCGRG 4 cut(s) 268, 288, 301, 3021
EcoICRI GAGCTC 1 cut(s) 3810
EcoNI CCTNNNNNAGG 1 cut(s) 1554
EcoO109I RGGNCCY 4 cut(s) 745, 805, 2777, 3775
EcoRI GAATTC 3 cut(s) 77, 3535, 3718
EcoRV GATATC 1 cut(s) 3011
EcoT14I CCWWGG 3 cut(s) 1288, 2288, 3366
EcoT22I ATGCAT 3 cut(s) 970, 1489, 2190
EcoT38I GRGCYC 2 cut(s) 2951, 3812
ErhI CCWWGG 3 cut(s) 1288, 2288, 3366
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FauI CCCGC 3 cut(s) 308, 1866, 3364
FauNDI CATATG 1 cut(s) 2461
FbaI TGATCA 2 cut(s) 1215, 2059
FblI GTMKAC 2 cut(s) 1650, 1988
FriOI GRGCYC 2 cut(s) 2951, 3812
FspBI CTAG 7 cut(s) 344, 1260, 1635, 1658, 2030, 2352, 2436
GlaI GCGC 3 cut(s) 277, 1245, 1630
GsaI CCCAGC 2 cut(s) 2112, 3549
GsuI CTGGAG 2 cut(s) 1956, 2889
HaeII RGCGCY 1 cut(s) 279
HgaI GACGC 8 cut(s) 24, 68, 237, 336, 347, 2236, 2752, 2834
HhaI GCGC 3 cut(s) 278, 1246, 1631
Hin1I GRCGYC 5 cut(s) 35, 60, 339, 2247, 2845
Hin6I GCGC 3 cut(s) 276, 1244, 1629
HinP1I GCGC 3 cut(s) 276, 1244, 1629
HincII GTYRAC 3 cut(s) 337, 1224, 2742
HindII GTYRAC 3 cut(s) 337, 1224, 2742
HindIII AAGCTT 2 cut(s) 1004, 1353
HpaI GTTAAC 1 cut(s) 1224
HphI GGTGA 7 cut(s) 539, 1051, 1160, 2069, 2242, 2461, 3407
Hpy99I CGWCG 5 cut(s) 40, 62, 253, 490, 1455
HpyCH4III ACNGT 2 cut(s) 629, 1567
HpyCH4IV ACGT 5 cut(s) 480, 524, 2668, 3157, 3435
HpySE526I ACGT 5 cut(s) 480, 524, 2668, 3157, 3435
Hsp92I GRCGYC 5 cut(s) 35, 60, 339, 2247, 2845
HspAI GCGC 3 cut(s) 276, 1244, 1629
KpnI GGTACC 1 cut(s) 2982
Ksp22I TGATCA 2 cut(s) 1215, 2059
KspAI GTTAAC 1 cut(s) 1224
KspI CCGCGG 1 cut(s) 205
LweI GCATC 8 cut(s) 235, 623, 883, 955, 1168, 1496, 2197, 2623
MabI ACCWGGT 3 cut(s) 549, 1144, 1465
MaeI CTAG 7 cut(s) 344, 1260, 1635, 1658, 2030, 2352, 2436
MaeII ACGT 5 cut(s) 480, 524, 2668, 3157, 3435
MaeIII GTNAC 6 cut(s) 476, 1567, 1803, 2248, 2404, 3487
MboII GAAGA 7 cut(s) 16, 572, 683, 881, 917, 2940, 3835
MfeI CAATTG 2 cut(s) 1581, 1811
MflI RGATCY 4 cut(s) 907, 1939, 2829, 3416
MlsI TGGCCA 1 cut(s) 2293
MluNI TGGCCA 1 cut(s) 2293
MlyI GAGTC 1 cut(s) 1994
MmeI TCCRAC 4 cut(s) 255, 2238, 3101, 3217
Mox20I TGGCCA 1 cut(s) 2293
Mph1103I ATGCAT 3 cut(s) 970, 1489, 2190
MroXI GAANNNNTTC 1 cut(s) 3575
MscI TGGCCA 1 cut(s) 2293
MslI CAYNNNNRTG 2 cut(s) 3675, 3834
Msp20I TGGCCA 1 cut(s) 2293
MspA1I CMGCKG 3 cut(s) 204, 826, 1277
MspCI CTTAAG 2 cut(s) 712, 2416
MunI CAATTG 2 cut(s) 1581, 1811
Mva1269I GAATGC 3 cut(s) 2015, 2190, 3691
MvnI CGCG 5 cut(s) 120, 186, 192, 198, 204
NciI CCSGG 7 cut(s) 110, 137, 269, 270, 289, 290, 458
NcoI CCATGG 3 cut(s) 1288, 2288, 3366
NdeI CATATG 1 cut(s) 2461
NheI GCTAGC 1 cut(s) 2351
NmuCI GTSAC 3 cut(s) 1567, 2248, 2404
NruI TCGCGA 1 cut(s) 120
NsiI ATGCAT 3 cut(s) 970, 1489, 2190
NspI RCATGY 4 cut(s) 431, 1214, 1926, 3379
OliI CACNNNNGTG 1 cut(s) 3675
PaeI GCATGC 1 cut(s) 431
PasI CCCWGGG 1 cut(s) 88
PciI ACATGT 2 cut(s) 1210, 1922
PcsI WCGNNNNNNNCGW 1 cut(s) 458
PctI GAATGC 3 cut(s) 2015, 2190, 3691
PdmI GAANNNNTTC 1 cut(s) 3575
PfeI GAWTC 9 cut(s) 5, 127, 161, 544, 1037, 1132, 2555, 2628, 3826
PflMI CCANNNNNTGG 5 cut(s) 946, 1068, 2975, 3044, 3083
PfoI TCCNGGA 1 cut(s) 2866
PleI GAGTC 1 cut(s) 1993
PmaCI CACGTG 1 cut(s) 2669
PmlI CACGTG 1 cut(s) 2669
PpsI GAGTC 1 cut(s) 1993
Ppu21I YACGTR 1 cut(s) 2669
PpuMI RGGWCCY 3 cut(s) 745, 805, 2777
PscI ACATGT 2 cut(s) 1210, 1922
PsiI TTATAA 3 cut(s) 2529, 3432, 3527
Psp124BI GAGCTC 1 cut(s) 3812
Psp1406I AACGTT 1 cut(s) 3435
Psp5II RGGWCCY 3 cut(s) 745, 805, 2777
PspCI CACGTG 1 cut(s) 2669
PspFI CCCAGC 2 cut(s) 2108, 3545
PspPPI RGGWCCY 3 cut(s) 745, 805, 2777
PsrI GAACNNNNNNTAC 2 cut(s) 3096, 3128
PstI CTGCAG 1 cut(s) 1579
PstNI CAGNNNCTG 1 cut(s) 2006
PsuI RGATCY 4 cut(s) 907, 1939, 2829, 3416
PvuII CAGCTG 2 cut(s) 826, 1277
RruI TCGCGA 1 cut(s) 120
RseI CAYNNNNRTG 2 cut(s) 3675, 3834
SacI GAGCTC 1 cut(s) 3812
SacII CCGCGG 1 cut(s) 205
SchI GAGTC 1 cut(s) 1994
SexAI ACCWGGT 3 cut(s) 549, 1144, 1465
SfaNI GCATC 8 cut(s) 235, 623, 883, 955, 1168, 1496, 2197, 2623
SfcI CTRYAG 3 cut(s) 697, 1575, 1647
Sfr303I CCGCGG 1 cut(s) 205
SgrBI CCGCGG 1 cut(s) 205
SmaI CCCGGG 2 cut(s) 270, 290
SmiMI CAYNNNNRTG 2 cut(s) 3675, 3834
SmlI CTYRAG 5 cut(s) 614, 712, 911, 2416, 3540
SmoI CTYRAG 5 cut(s) 614, 712, 911, 2416, 3540
SphI GCATGC 1 cut(s) 431
SrfI GCCCGGGC 1 cut(s) 290
SspMI CTAG 7 cut(s) 344, 1260, 1635, 1658, 2030, 2352, 2436
SstI GAGCTC 1 cut(s) 3812
StyI CCWWGG 3 cut(s) 1288, 2288, 3366
TaaI ACNGT 2 cut(s) 629, 1567
TaiI ACGT 5 cut(s) 483, 527, 2671, 3160, 3438
TaqII GACCGA 1 cut(s) 120
TatI WGTACW 3 cut(s) 1319, 1857, 3726
TauI GCSGC 5 cut(s) 207, 315, 639, 1162, 3793
TfiI GAWTC 9 cut(s) 5, 127, 161, 544, 1037, 1132, 2555, 2628, 3826
TscAI CASTG 8 cut(s) 385, 761, 797, 1368, 1449, 1872, 2069, 3169
TseFI GTSAC 3 cut(s) 1567, 2248, 2404
Tsp45I GTSAC 3 cut(s) 1567, 2248, 2404
TspGWI ACGGA 2 cut(s) 1424, 1955
TspMI CCCGGG 2 cut(s) 268, 288
TspRI CASTG 8 cut(s) 385, 761, 797, 1368, 1449, 1872, 2069, 3169
Van91I CCANNNNNTGG 5 cut(s) 946, 1068, 2975, 3044, 3083
Vha464I CTTAAG 2 cut(s) 712, 2416
VneI GTGCAC 1 cut(s) 1461
XagI CCTNNNNNAGG 1 cut(s) 1554
XapI RAATTY 5 cut(s) 77, 1954, 2204, 3535, 3718
XceI RCATGY 4 cut(s) 431, 1214, 1926, 3379
XmaI CCCGGG 2 cut(s) 268, 288
XmiI GTMKAC 2 cut(s) 1650, 1988
XmnI GAANNNNTTC 1 cut(s) 3575
XspI CTAG 7 cut(s) 344, 1260, 1635, 1658, 2030, 2352, 2436
Zsp2I ATGCAT 3 cut(s) 970, 1489, 2190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.