AT5G49110

FANCI solenoid 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009455
Physical Location & Seq
Forward (+)
19900150 .. 19906171
6022 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49110.1

Sequence Viewer

Length: 4410 bp
ATGACCGTCGCCGCCGCAGCCGAAGAACCATCTCAGAGACCTCTCACCGACAAAGATATCATTCGTTTAGCACAGTACCACCATTGTCAAACCGGATTCTCTCTCCCACCTTACCTTCTCTCACCGTCTTCCCACGATTCCCTTCTCTCATACCTCAAATCTCGCTCTTCTTCACCATCTCCGTCGAAACAAGTTTCTGAGTATGTAATCGCTCTTCTATCTCTAATATCTCTCTCCCCTACTACTCCCTCTCTCTGCTCTCTCCTCGCTTCTCTTCTCATCTCCTACACCCAAATCTTCAATTCCTGCAAAATCCCAAGCGATTCTGATTCTCTCAAGACGATTCAGCTCTTCGGTACTCTCTTACGATACTTACACGTTAAGGAGGTGAAATCTGTAGTTGATTCGATCTTATCAGGTGTGACTAGAGTTATCACTGTTGATGAAGCCCAGGTGTTCGATTTATTGCCTGTGTGCTTTGATTTATTGCGTAGAGAAGGTAAAGCTAGTGAAATCGATTTCGTTAATTCGGCGATTGATCGTGTTCTTAGTTGTGAATGGAACAAAGCGTTGTTGACGAAAATGGTTTCTCTTGCAAAGGAGTTTTCATTTCTGGATAAAGGGAGAAAAAGTGAATTGGTAGAGAAAGTGTTTGTTGGTATTAAGTTGATAGACTTGCAGGATTTGCCTTCACTTGTGTATCAATTGTTGGTTCTTGCCTCTAAAGGTTTTTGCAAGAGGGAAGTGATTGGTGGTGTTGTTGGTTACTTTGGGTCTAAAGCTGAGACTAGAGTAGCTTCCGTTGTTAGACAGATAGAAGGAACAGTTCTTCTTCATGTGAATTTTGCTGTGAAGCAGGATCCTTCATTGGGGCAAGAGGTTGTGGCGTTAGTCAAATCTGATCTTAGAGCTTTCAATCATTTTACAGTCGCGGTTCTGTTTTCCGTTGCTCGGGTTAGAAAATTCGGTGAGAGCTCCTTGGGGATGTTGAGAACGGCTTTGCTTACTGCGTACAATGATTACAGACTCTCCAAAGATTGCAAGTGGCTACCTGTTGAGCTTAAGGAAGAGAGTTTCCTGCATGCCAAATTGGTTGAAAAATCTTTGCTAAGAGCGGTTAGTGAGTGCCGATATGGGAGAGAGCATGTGGTTCCAAGTGTTATTCAGTTTGGCTTTATGTTGCTAGAATCTGTTGAAGAAGGTAGATCCAATGAGTTTGGTGATTCGAAAGGTGTACTCGGTATTGAGAAGCTTAGCATTCAGATTCTGGGAACCCTATTTGAAGTCCATGATATGACGAGAAACGAGATAATTGAGCAATGCAAATTTCGCATTCTTTCTTTGAAATGCGCAAAGAGCAAGCCAATAGTAAGGTTGTTAGGGTATCTTGTCCAGAGATACTCACTTATCATGTTGGAATTTGTCCACCATCTCAAAGAGTTATTGGATTATTTCACTTTCATGGAGGGACATATCAGCTGTTTTCTTGTTTCTGCTGTTATCCCGCTCATCAAATTCAGCCGTGATCTTCAGGACTACACCATCTTGGTGATTCGCAAAGCTATGTTTAGACGGGAAGATACAGTTCGTGTTGCAGCAACCAAAGTAATAACTGATCTTATACTTGCAGAGAAACTAGCTAAGAGAGACAGCTCATTTACTTTCCAAGACTCAGCAAGCCAAGCCAGTTCCAGTCAGCAGACTGAGATGAGCTGTATTGTGAGGGGAAACCTCTTTACTGAACTGAATGGGTTGCTTCAAAGATGTCTTTACCAGCAGGCAAGAGTGAAGGAAGTAGTTTATGATGGGCTTGTGAAGTTAGTCCTGATTGATCCATCAAGTGGAGGACATGTGTTGGACTTTCTTATGCCTCATTTTCTTCGTTTCTTCGGACAGGACACAGATTTTCAACTTGGAATCACTTCATGCATTAGAGTAGAAGGTGACAAATTTACTATTGAAGAGCCCTTGGATAGGCTTCTCTTTTGTGTCTCTTGGATTTTACTTCTCCAGCAGCAAAACAGCTCTGATCGGCCTTCCGATGCTGCGTGGCCATGCTTTGGTTTCTCCCTTTCACAAGATAATGAGCAGGTTGGAAGAAACCTATCTCATGAAGCGTACTCCAGTGCTTTGGTAAAGATCCGAAGCTTCTTACTAGGCAAGAAGTTAGGAGATATTGTAGGGCAGTCTCAGGACACAGTTTCTGCATCTCTCGAAGAAGATAAAAGAAAATCCTATTGTTTGATTATATTGGGAATCGTTCAAGTGCTGCTAAATTATATTATAGTGGATCTAGAGAAACAGCCAGAAGGGAAAAAGGGTTCTGTCAGAAAGGAGATTGTCGACTTAATTGATCTTTATGAATCACTGGAAAAGGATGTGGGAAAATCGAAGCAGAGCAATGTTGGCAAGAGAGTACGATTTAGCGCCTGTAATGATACTGATCTGGGAAACACGAGTATCAATGAAGAGCGAGAGAAAGTTCCCTTTTTGGCTACTTCAAGTATCTACCAGCTATTTCTACTCAGTTTCAAACTCTATAGCAGCAAATCCGTTGGTACTCAGTCAGGCTCGCAGGATCACAGCCATTCATCACCAGCGAAGACAGAAAAATCAATTTTCTCCTTCACTTTGCATGTTTGTGTTGGGCACATAAATTCATCTCTTTGCATGAAGGAGGAAAATCCACTTAAACCGTTGGTCTGTGGTGACATGAAGGTTCTGGGTCCTCCACTGCTAAAAGTAGTTTACCTGCTAAAGCCAGAGCCACCTCTAGCTACTGGACAGACGAATAAGGAGAAGAAAGGAAGGAAAGATGTTGACGGAAGAAAACAGTGTCTACATCTGGCCTTACTTAGCTTGAAAGAGCTACTCAATATATATTCAAGTGGATCTGGTTTGACTGGCTTGCTTGAAGATTTGTTGGCAGTACCTGCATCCGAGGATGCTACTTTAGAAGAGTGCAGGGAAGCTTCAAAAATTGAAGATCCACTTATAAAGAGCATCGAAATTTTCATGGCGAAAATCATGAAGCCCATGATCACAGACTTTATCGCGCAAAACTCTAATGACGTAGAGGAAGCTAAGACCATAATATTCAATTGCACTGTTCAGATTCTTTGTGACATAATGTTGAAGCTTGGGAACAATCTACCAGACAAATTCAAACAAAGGCATGGATCATGGGCTCATCAAATCTGCAGAAGCTGTGAAACATCTAACACCACTGTGGCAAAGAGCGTTCTGAAACTTGCCATCTCTTTTACTACATCCCCTGGTGATCTATGCATAGCCGTGGAAGTGGCTCAGGAGTTGCAAAATATCATGGGTTTAGACAAATCTGATACGTTAGAAGTGTCTGAGTCATACATGGTCATAAACCAGTCAACTAGTGCTTCCGTAACTTCTTGTATATTACAGTCTATTGATTCAGCAATAGTTGACATGGATTGGGCCACAAAGAAGCTAAAGAACTTTTATGTGGTCTCTCAAAAGAACATCCATCTCAGCGATGATACTGAGAGCACAGTCGGGTCGGTTCTCGAGGAAGCCCTTTACTCAATGGCTGAGTCAACAGTCAGAATACTTTCTTCCTTTGTTTTGATGAACCTCAAAGAGTCGCAAGCAGCGCAGTTTCTTAGACTAGCAGTTAGGTTTTATAAACAATTGGCACAAATTGTGAAGCTGAGGATTGCTCCCAAGGGTTGCAAGCAAATTCTTCCTAGCCTTAAATTTCAAAAACTCGTGGAACTGACTTGCAAGAGTCTCACGGTTCCTTTATATCCCTTTTTATCTGAAATGCAAAAGGTAAAAGTTGTTCAAGCAGTCATTACATTGATGTTTCTTCGTATCCAAAAGCGTGAAGTGTTGCTTCTTTTCCAAATTCAGGAACAACAAGAAAGTGTGAGTCACAATTCCAAGGGAATAATCAATAAGATCAAACAGGAGAACAAATGCATTCCTGATTTGATATTCCAGATAGAAGACTGTGAAAGATACCTAATTCAGCTAAGCAAAGTTACTAAATTGAATTTGTTGAGGCATGCCAAGCGCAGCACTGCCAGAGACTTCAAAATAATTGAAGATTCAGACCCTCCTGCAGGAGGCGAAGATGGAGGAAACCAGGAAGAAGAGACAGAGACACAGAACAATAATATTGGCGAGGATGATTTAAGACAAGAGTCAGATGATGATGGTTCAGAAGAAATGTTGTCCCCCCGAAATGCTAGTTCAGTATCATCACCAGGATTAGATTCTGATAAAACAATAGCAGCAGCAACAGAAGAGGACGATGAAGAAGAACAAGAACAAGATGAAGGAGAAGAAGAAAGTGGCGATAATCGTCCTAAAAAGATGGCTAGGAAGAGTTGGGTTGTGGAAGATTCTGATGAAGATTCTGAAACATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
FANCI_HD1 FANCI_HD2 FANCI_S1 FANCI_S2 FANCI_S4

Protein Analysis

1469

Amino Acids

164.23

Weight (kDa)

5.77

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FANCI_S1 PF14675 133 - 285 3.3e-13 FANCI solenoid 1
FANCI_HD1 PF14679 289 - 369 4.4e-20 FANCI helical domain 1
FANCI_S2 PF14676 383 - 536 1.8e-50 FANCI solenoid 2
FANCI_HD2 PF14680 556 - 793 1.2e-63 FANCI helical domain 2
FANCI_S4 PF14678 1098 - 1362 5.4e-66 FANCI solenoid 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2996, 3660
Acc16I TGCGCA 1 cut(s) 1351
Acc36I ACCTGC 3 cut(s) 2080, 2760, 2941
AccB7I CCANNNNNTGG 2 cut(s) 1841, 2060
AccBSI CCGCTC 2 cut(s) 1115, 1507
AccI GTMKAC 2 cut(s) 2343, 2839
AccII CGCG 2 cut(s) 932, 3056
AciI CCGC 5 cut(s) 12, 15, 932, 1115, 1505
AcoI YGGCCR 1 cut(s) 2051
AcuI CTGAAG 1 cut(s) 1514
AfaI GTAC 8 cut(s) 77, 358, 1013, 1236, 2120, 2418, 2560, 2931
AfiI CCNNNNNNNGG 8 cut(s) 869, 951, 1841, 1974, 2060, 3886, 4100, 4103
AflII CTTAAG 1 cut(s) 1061
AflIII ACRYGT 2 cut(s) 376, 1849
AhlI ACTAGT 1 cut(s) 3389
AjnI CCWGG 4 cut(s) 450, 3274, 4122, 4243
AleI CACNNNNGTG 1 cut(s) 3227
AloI GAACNNNNNNTCC 2 cut(s) 2305, 2337
Alw21I GWGCWC 2 cut(s) 977, 3527
AlwNI CAGNNNCTG 4 cut(s) 1267, 2204, 2933, 3207
Ama87I CYCGRG 2 cut(s) 951, 3542
AoxI GGCC 4 cut(s) 2033, 2051, 2847, 3453
Asp700I GAANNNNTTC 2 cut(s) 1921, 3586
AspLEI GCGC 5 cut(s) 1352, 2429, 3058, 3631, 4053
AspS9I GGNCC 2 cut(s) 2726, 3453
AsuII TTCGAA 1 cut(s) 1226
AvaI CYCGRG 2 cut(s) 951, 3542
AvaII GGWCC 1 cut(s) 2726
BaeGI GKGCMC 1 cut(s) 2652
BaeI ACNNNNGTAYC 4 cut(s) 683, 716, 2443, 2476
BalI TGGCCA 1 cut(s) 2053
BamHI GGATCC 1 cut(s) 859
BanII GRGCYC 3 cut(s) 977, 1968, 3190
BarI GAAGNNNNNNTAC 2 cut(s) 198, 230
BauI CACGAG 2 cut(s) 2455, 3743
BbsI GAAGAC 3 cut(s) 120, 2609, 3990
Bbv12I GWGCWC 2 cut(s) 977, 3527
BbvCI CCTCAGC 1 cut(s) 3686
BceAI ACGGC 3 cut(s) 1011, 1506, 3278
BciT130I CCWGG 4 cut(s) 452, 3276, 4124, 4245
BciVI GTATCC 1 cut(s) 3860
BclI TGATCA 1 cut(s) 3039
BcuI ACTAGT 1 cut(s) 3389
BfmI CTRYAG 4 cut(s) 396, 2539, 3199, 4098
BfoI RGCGCY 1 cut(s) 2430
BfrI CTTAAG 1 cut(s) 1061
BfuAI ACCTGC 3 cut(s) 2080, 2760, 2941
BfuI GTATCC 1 cut(s) 3860
BlpI GCTNAGC 2 cut(s) 1253, 4010
Bme1390I CCNGG 4 cut(s) 452, 3276, 4124, 4245
Bme18I GGWCC 1 cut(s) 2726
BmeT110I CYCGRG 2 cut(s) 951, 3542
BmgT120I GGNCC 2 cut(s) 2726, 3453
BmiI GGNNCC 5 cut(s) 861, 1152, 1273, 2727, 3774
BmrFI CCNGG 4 cut(s) 452, 3276, 4124, 4245
BmsI GCATC 5 cut(s) 2032, 2216, 2935, 2945, 3012
BoxI GACNNNNGTC 1 cut(s) 4180
BpiI GAAGAC 3 cut(s) 120, 2609, 3990
BplI GAGNNNNNCTC 2 cut(s) 3679, 3711
BpmI CTGGAG 2 cut(s) 1994, 2107
Bpu10I CCTNAGC 2 cut(s) 3306, 3686
Bpu1102I GCTNAGC 2 cut(s) 1253, 4010
Bpu14I TTCGAA 1 cut(s) 1226
BpuEI CTTGAG 1 cut(s) 320
Bsa29I ATCGAT 1 cut(s) 516
BsaBI GATNNNNATC 1 cut(s) 2442
BsaI GGTCTC 2 cut(s) 31, 3490
BsaJI CCNNGG 8 cut(s) 450, 978, 1968, 2940, 3274, 3294, 3699, 3918
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 8 cut(s) 616, 646, 1013, 1043, 1836, 1866, 2163, 2193
Bsc4I CCNNNNNNNGG 8 cut(s) 869, 951, 1841, 1974, 2060, 3886, 4100, 4103
Bse1I ACTGG 7 cut(s) 1686, 1692, 2124, 2373, 2785, 2908, 3382
Bse3DI GCAATG 2 cut(s) 1325, 2407
Bse8I GATNNNNATC 1 cut(s) 2442
BseBI CCWGG 4 cut(s) 452, 3276, 4124, 4245
BseCI ATCGAT 1 cut(s) 516
BseDI CCNNGG 8 cut(s) 450, 978, 1968, 2940, 3274, 3294, 3699, 3918
BseGI GGATG 7 cut(s) 990, 2383, 2936, 2950, 3269, 3498, 4171
BseJI GATNNNNATC 1 cut(s) 2442
BseLI CCNNNNNNNGG 8 cut(s) 869, 951, 1841, 1974, 2060, 3886, 4100, 4103
BseMI GCAATG 2 cut(s) 1325, 2407
BseNI ACTGG 7 cut(s) 1686, 1692, 2124, 2373, 2785, 2908, 3382
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 2652
BsgI GTGCAG 1 cut(s) 2983
Bsh1236I CGCG 2 cut(s) 932, 3056
BshFI GGCC 4 cut(s) 2035, 2053, 2849, 3455
BshVI ATCGAT 1 cut(s) 516
BsiHKAI GWGCWC 2 cut(s) 977, 3527
BsiHKCI CYCGRG 2 cut(s) 951, 3542
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 1482, 4198
BslI CCNNNNNNNGG 8 cut(s) 869, 951, 1841, 1974, 2060, 3886, 4100, 4103
BsmFI GGGAC 2 cut(s) 1482, 4198
BsmI GAATGC 3 cut(s) 1257, 1332, 3957
BsnI GGCC 4 cut(s) 2035, 2053, 2849, 3455
Bso31I GGTCTC 2 cut(s) 31, 3490
BsoBI CYCGRG 2 cut(s) 951, 3542
Bsp119I TTCGAA 1 cut(s) 1226
Bsp1286I GDGCHC 5 cut(s) 977, 1968, 2652, 3190, 3527
Bsp1720I GCTNAGC 2 cut(s) 1253, 4010
BspACI CCGC 5 cut(s) 12, 15, 932, 1115, 1505
BspANI GGCC 4 cut(s) 2035, 2053, 2849, 3455
BspDI ATCGAT 1 cut(s) 516
BspFNI CGCG 2 cut(s) 932, 3056
BspHI TCATGA 2 cut(s) 2110, 3027
BspLI GGNNCC 5 cut(s) 861, 1152, 1273, 2727, 3774
BspMAI CTGCAG 2 cut(s) 3203, 4102
BspMI ACCTGC 3 cut(s) 2080, 2760, 2941
BspQI GCTCTTC 5 cut(s) 172, 219, 356, 1956, 2463
BspT104I TTCGAA 1 cut(s) 1226
BspTI CTTAAG 1 cut(s) 1061
BspTNI GGTCTC 2 cut(s) 31, 3490
BsrBI CCGCTC 2 cut(s) 1115, 1507
BsrDI GCAATG 2 cut(s) 1325, 2407
BsrI ACTGG 7 cut(s) 1686, 1692, 2124, 2373, 2785, 2908, 3382
BssECI CCNNGG 8 cut(s) 450, 978, 1968, 2940, 3274, 3294, 3699, 3918
BssSI CACGAG 2 cut(s) 2455, 3743
BssT1I CCWWGG 4 cut(s) 978, 1968, 3699, 3918
Bst2BI CACGAG 2 cut(s) 2455, 3743
Bst2UI CCWGG 4 cut(s) 452, 3276, 4124, 4245
BstAFI CTTAAG 1 cut(s) 1061
BstAPI GCANNNNNTGC 2 cut(s) 685, 2933
BstBI TTCGAA 1 cut(s) 1226
BstC8I GCNNGC 9 cut(s) 1083, 1361, 1678, 1779, 2573, 2909, 3624, 3710, 4044
BstDSI CCRYGG 1 cut(s) 3294
BstENI CCTNNNNNAGG 3 cut(s) 1972, 4098, 4101
BstF5I GGATG 7 cut(s) 990, 2383, 2936, 2950, 3269, 3498, 4171
BstFNI CGCG 2 cut(s) 932, 3056
BstH2I RGCGCY 1 cut(s) 2430
BstHHI GCGC 5 cut(s) 1352, 2429, 3058, 3631, 4053
BstMWI GCNNNNNNNGC 9 cut(s) 17, 685, 1329, 1356, 1682, 2406, 2933, 3628, 4048
BstNI CCWGG 4 cut(s) 452, 3276, 4124, 4245
BstNSI RCATGY 5 cut(s) 1085, 1148, 1853, 2639, 4046
BstPAI GACNNNNGTC 1 cut(s) 4180
BstSCI CCNGG 4 cut(s) 450, 3274, 4122, 4243
BstSFI CTRYAG 4 cut(s) 396, 2539, 3199, 4098
BstSLI GKGCMC 1 cut(s) 2652
BstUI CGCG 2 cut(s) 932, 3056
BstV2I GAAGAC 3 cut(s) 120, 2609, 3990
BstX2I RGATCY 6 cut(s) 859, 1205, 2139, 2290, 2891, 2986
BstXI CCANNNNNNTGG 1 cut(s) 2131
BstYI RGATCY 6 cut(s) 859, 1205, 2139, 2290, 2891, 2986
Bsu15I ATCGAT 1 cut(s) 516
BsuI GTATCC 1 cut(s) 3860
BsuRI GGCC 4 cut(s) 2035, 2053, 2849, 3455
BsuTUI ATCGAT 1 cut(s) 516
BtgI CCRYGG 1 cut(s) 3294
BtgZI GCGATG 1 cut(s) 3525
BtsCI GGATG 7 cut(s) 990, 2383, 2936, 2950, 3269, 3498, 4171
BtsI GCAGTG 2 cut(s) 2732, 4056
BtsIMutI CAGTG 8 cut(s) 435, 2131, 2366, 2732, 2840, 3105, 3225, 4056
BveI ACCTGC 3 cut(s) 2080, 2760, 2941
Cac8I GCNNGC 9 cut(s) 1083, 1361, 1678, 1779, 2573, 2909, 3624, 3710, 4044
CaiI CAGNNNCTG 4 cut(s) 1267, 2204, 2933, 3207
CciI TCATGA 2 cut(s) 2110, 3027
CfoI GCGC 5 cut(s) 1352, 2429, 3058, 3631, 4053
Cfr13I GGNCC 2 cut(s) 2726, 3453
ClaI ATCGAT 1 cut(s) 516
Csp6I GTAC 8 cut(s) 76, 357, 1012, 1235, 2119, 2417, 2559, 2930
CviQI GTAC 8 cut(s) 76, 357, 1012, 1235, 2119, 2417, 2559, 2930
EaeI YGGCCR 1 cut(s) 2051
Ecl136II GAGCTC 1 cut(s) 975
Eco130I CCWWGG 4 cut(s) 978, 1968, 3699, 3918
Eco24I GRGCYC 3 cut(s) 977, 1968, 3190
Eco31I GGTCTC 2 cut(s) 31, 3490
Eco32I GATATC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 2726
Eco53kI GAGCTC 1 cut(s) 975
Eco57I CTGAAG 1 cut(s) 1514
Eco88I CYCGRG 2 cut(s) 951, 3542
EcoICRI GAGCTC 1 cut(s) 975
EcoNI CCTNNNNNAGG 3 cut(s) 1972, 4098, 4101
EcoO109I RGGNCCY 1 cut(s) 2726
EcoRII CCWGG 4 cut(s) 450, 3274, 4122, 4243
EcoRV GATATC 1 cut(s) 58
EcoT14I CCWWGG 4 cut(s) 978, 1968, 3699, 3918
EcoT22I ATGCAT 3 cut(s) 1931, 3290, 3959
EcoT38I GRGCYC 3 cut(s) 977, 1968, 3190
ErhI CCWWGG 4 cut(s) 978, 1968, 3699, 3918
FalI AAGNNNNNCTT 4 cut(s) 2976, 3008, 3855, 3887
FaqI GGGAC 2 cut(s) 1482, 4198
FauI CCCGC 1 cut(s) 1512
FbaI TGATCA 1 cut(s) 3039
FblI GTMKAC 2 cut(s) 2343, 2839
FokI GGATG 7 cut(s) 997, 2390, 2923, 2957, 3256, 3485, 4178
FriOI GRGCYC 3 cut(s) 977, 1968, 3190
FspI TGCGCA 1 cut(s) 1351
GlaI GCGC 5 cut(s) 1351, 2428, 3057, 3630, 4052
GsuI CTGGAG 2 cut(s) 1994, 2107
HaeII RGCGCY 1 cut(s) 2430
HaeIII GGCC 4 cut(s) 2035, 2053, 2849, 3455
HapII CCGG 1 cut(s) 93
HhaI GCGC 5 cut(s) 1352, 2429, 3058, 3631, 4053
Hin6I GCGC 5 cut(s) 1350, 2427, 3056, 3629, 4051
HinP1I GCGC 5 cut(s) 1350, 2427, 3056, 3629, 4051
HincII GTYRAC 6 cut(s) 576, 2344, 2821, 3387, 3442, 3573
HindII GTYRAC 6 cut(s) 576, 2344, 2821, 3387, 3442, 3573
HindIII AAGCTT 4 cut(s) 1250, 2146, 2970, 3137
HpaII CCGG 1 cut(s) 93
Hpy99I CGWCG 2 cut(s) 11, 187
HpyCH4IV ACGT 3 cut(s) 378, 3072, 3347
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 685, 1329, 1356, 1682, 2406, 2933, 3628, 4048
HpySE526I ACGT 3 cut(s) 378, 3072, 3347
HspAI GCGC 5 cut(s) 1350, 2427, 3056, 3629, 4051
Ksp22I TGATCA 1 cut(s) 3039
LguI GCTCTTC 5 cut(s) 172, 219, 356, 1956, 2463
LmnI GCTCC 2 cut(s) 980, 3700
LweI GCATC 5 cut(s) 2032, 2216, 2935, 2945, 3012
MaeII ACGT 3 cut(s) 378, 3072, 3347
MaeIII GTNAC 8 cut(s) 421, 764, 1943, 2708, 3122, 3400, 3908, 4018
MbiI CCGCTC 2 cut(s) 1115, 1507
MfeI CAATTG 3 cut(s) 704, 3100, 3665
MflI RGATCY 6 cut(s) 859, 1205, 2139, 2290, 2891, 2986
MhlI GDGCHC 5 cut(s) 977, 1968, 2652, 3190, 3527
MlsI TGGCCA 1 cut(s) 2053
MluNI TGGCCA 1 cut(s) 2053
MlyI GAGTC 8 cut(s) 1020, 1664, 3371, 3578, 3626, 3772, 3916, 4190
MmeI TCCRAC 3 cut(s) 1395, 1836, 2074
Mox20I TGGCCA 1 cut(s) 2053
Mph1103I ATGCAT 3 cut(s) 1931, 3290, 3959
MroXI GAANNNNTTC 2 cut(s) 1921, 3586
MscI TGGCCA 1 cut(s) 2053
MseI TTAA 8 cut(s) 381, 525, 663, 1062, 2348, 2691, 3729, 4172
MslI CAYNNNNRTG 5 cut(s) 1460, 1547, 2640, 3227, 3293
Msp20I TGGCCA 1 cut(s) 2053
MspA1I CMGCKG 1 cut(s) 1479
MspCI CTTAAG 1 cut(s) 1061
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 4 cut(s) 452, 3276, 4124, 4245
MunI CAATTG 3 cut(s) 704, 3100, 3665
Mva1269I GAATGC 3 cut(s) 1257, 1332, 3957
MvaI CCWGG 4 cut(s) 452, 3276, 4124, 4245
MvnI CGCG 2 cut(s) 932, 3056
MwoI GCNNNNNNNGC 9 cut(s) 17, 685, 1329, 1356, 1682, 2406, 2933, 3628, 4048
NlaIV GGNNCC 5 cut(s) 861, 1152, 1273, 2727, 3774
NmuCI GTSAC 5 cut(s) 421, 1943, 2708, 3122, 3908
NsbI TGCGCA 1 cut(s) 1351
NsiI ATGCAT 3 cut(s) 1931, 3290, 3959
NspI RCATGY 5 cut(s) 1085, 1148, 1853, 2639, 4046
NspV TTCGAA 1 cut(s) 1226
OliI CACNNNNGTG 1 cut(s) 3227
PaeI GCATGC 2 cut(s) 1085, 4046
PaeR7I CTCGAG 1 cut(s) 3542
PagI TCATGA 2 cut(s) 2110, 3027
PciI ACATGT 1 cut(s) 1849
PciSI GCTCTTC 5 cut(s) 172, 219, 356, 1956, 2463
PctI GAATGC 3 cut(s) 1257, 1332, 3957
PdmI GAANNNNTTC 2 cut(s) 1921, 3586
PflMI CCANNNNNTGG 2 cut(s) 1841, 2060
PleI GAGTC 8 cut(s) 1020, 1664, 3370, 3577, 3625, 3771, 3915, 4189
PpsI GAGTC 8 cut(s) 1020, 1664, 3370, 3577, 3625, 3771, 3915, 4189
PpuMI RGGWCCY 1 cut(s) 2726
PscI ACATGT 1 cut(s) 1849
PshAI GACNNNNGTC 1 cut(s) 4180
PsiI TTATAA 2 cut(s) 2996, 3660
Psp124BI GAGCTC 1 cut(s) 977
Psp5II RGGWCCY 1 cut(s) 2726
Psp6I CCWGG 4 cut(s) 450, 3274, 4122, 4243
PspGI CCWGG 4 cut(s) 450, 3274, 4122, 4243
PspN4I GGNNCC 5 cut(s) 861, 1152, 1273, 2727, 3774
PspPI GGNCC 2 cut(s) 2726, 3453
PspPPI RGGWCCY 1 cut(s) 2726
PsrI GAACNNNNNNTAC 2 cut(s) 3801, 3833
PstI CTGCAG 2 cut(s) 3203, 4102
PstNI CAGNNNCTG 4 cut(s) 1267, 2204, 2933, 3207
PsuI RGATCY 6 cut(s) 859, 1205, 2139, 2290, 2891, 2986
PvuII CAGCTG 1 cut(s) 1479
RsaI GTAC 8 cut(s) 77, 358, 1013, 1236, 2120, 2418, 2560, 2931
RsaNI GTAC 8 cut(s) 76, 357, 1012, 1235, 2119, 2417, 2559, 2930
RseI CAYNNNNRTG 5 cut(s) 1460, 1547, 2640, 3227, 3293
SacI GAGCTC 1 cut(s) 977
SalI GTCGAC 1 cut(s) 2342
SapI GCTCTTC 5 cut(s) 172, 219, 356, 1956, 2463
SaqAI TTAA 8 cut(s) 381, 525, 663, 1062, 2348, 2691, 3729, 4172
Sau96I GGNCC 2 cut(s) 2726, 3453
SbfI CCTGCAGG 1 cut(s) 4102
SchI GAGTC 8 cut(s) 1020, 1664, 3371, 3578, 3626, 3772, 3916, 4190
ScrFI CCNGG 4 cut(s) 452, 3276, 4124, 4245
SdaI CCTGCAGG 1 cut(s) 4102
SduI GDGCHC 5 cut(s) 977, 1968, 2652, 3190, 3527
SfaNI GCATC 5 cut(s) 2032, 2216, 2935, 2945, 3012
SfcI CTRYAG 4 cut(s) 396, 2539, 3199, 4098
Sfr274I CTCGAG 1 cut(s) 3542
SfuI TTCGAA 1 cut(s) 1226
SinI GGWCC 1 cut(s) 2726
SlaI CTCGAG 1 cut(s) 3542
SmiMI CAYNNNNRTG 5 cut(s) 1460, 1547, 2640, 3227, 3293
SmlI CTYRAG 3 cut(s) 335, 1061, 3542
SmoI CTYRAG 3 cut(s) 335, 1061, 3542
SpeI ACTAGT 1 cut(s) 3389
SphI GCATGC 2 cut(s) 1085, 4046
Sse8387I CCTGCAGG 1 cut(s) 4102
SsiI CCGC 5 cut(s) 12, 15, 932, 1115, 1505
SspI AATATT 2 cut(s) 3096, 4156
SstI GAGCTC 1 cut(s) 977
StyD4I CCNGG 4 cut(s) 450, 3274, 4122, 4243
StyI CCWWGG 4 cut(s) 978, 1968, 3699, 3918
TaiI ACGT 3 cut(s) 381, 3075, 3350
TatI WGTACW 1 cut(s) 1234
TauI GCSGC 2 cut(s) 14, 17
Tru1I TTAA 8 cut(s) 381, 525, 663, 1062, 2348, 2691, 3729, 4172
Tru9I TTAA 8 cut(s) 381, 525, 663, 1062, 2348, 2691, 3729, 4172
TscAI CASTG 8 cut(s) 442, 2131, 2373, 2739, 2840, 3112, 3232, 4063
TseFI GTSAC 5 cut(s) 421, 1943, 2708, 3122, 3908
Tsp45I GTSAC 5 cut(s) 421, 1943, 2708, 3122, 3908
TspGWI ACGGA 6 cut(s) 171, 790, 934, 2542, 2838, 3388
TspRI CASTG 8 cut(s) 442, 2131, 2373, 2739, 2840, 3112, 3232, 4063
Van91I CCANNNNNTGG 2 cut(s) 1841, 2060
Vha464I CTTAAG 1 cut(s) 1061
VpaK11BI GGWCC 1 cut(s) 2726
XagI CCTNNNNNAGG 3 cut(s) 1972, 4098, 4101
XbaI TCTAGA 1 cut(s) 2293
XceI RCATGY 5 cut(s) 1085, 1148, 1853, 2639, 4046
XhoI CTCGAG 1 cut(s) 3542
XmiI GTMKAC 2 cut(s) 2343, 2839
XmnI GAANNNNTTC 2 cut(s) 1921, 3586
Zsp2I ATGCAT 3 cut(s) 1931, 3290, 3959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.