MD13G1166500.v1.1

FANCI solenoid 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009455
Physical Location & Seq
Reverse (-)
13323000 .. 13329480
6481 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1166500.v1.1.491

Sequence Viewer

Length: 4230 bp
ATGAACACCACCGCAACCATATCCACCAGGGAACAACAATCACCACCCCCACTATCGGACACCGATATCATCCGAGTAGCCCAGTCCTGCTCCCCAATCCCCACCTACCTTCTCTCCACAGCGTCCCACCCAACCCTCCTCTCTTACCTCACAGCCCGCGCTCAATCCACTTCGCCGTCGAGGCCAGTCTCCGACTACACCGTCTCTCTCCTCTCCCTCATCGCTCTGTCTCCCCAATTCGATTCTCTCTCTACCCTCCTCTCCTCTCTCCTCCGATCCTACACCCAAATCTTCACCTCCTTCGGAATCCCACACGACGCCAACTCCCTCAAAACCATCCAATTCTTCCACCCCCTCTTGCCCCACGTACCCATCACCAATCTTCCCCAAATAGTCGAACCCATTGTGTCATTTTTGCCCCAAATCGTCGATTTGGAGGATGCCCAAATCCTTGACCTTCTTCCCCGGTGCTTGAACTTGATTCGCAACGCTGAGGAGATTGAACGCGGCGGGCATTTTGTCAATTCGGTGATTGATAAGATTCTGGAGGTTAATTGGTCAAAAGGGTTGGTGATCAAGATGGTTTCGATTGTCAGGGACTTTCCGGTTCTAGATAAAACGAGGGCTAGGGAGTTTGTAGACAAGGTGTTTGTGGGAATGTCAAGGTTAGACTTGCAGGACTTGCCGTCGATTGTGTACCAGCTGCTGGTTTTGGCGTCTAAAGGGTTTGTTAAGAGAGAGGTAGTTGAAGGGATTGTGATGTTTTTCGGGTCGAAAATGGCGTCAAAGGCAACCTCTATTGTTAGACAGGTTGAAGGGACTGTGTTGCTTCATGTGAACTTTGCAGTAAAGCAGGATCCTACACTAGGACAAGAGGTGATGGGACTTGTGAGGTCGGATCTTAGAGCTTTCAATCACTTTACAGTTGCTGTTTTGTTGTCGGTTTCGAGGGTTCGGAAGTTTGGTGAGAGCTCGATGGGGGCTTTGAAAGTTGCGCTGCTTACCGCGTACCGTGATTACAAGTTTGCTAAAGATTGTAAGTGGATACCTAATGACATGAAGGAAGAACATCTGCAGAATGTCAAAATAGTGGAAAAGGCCATATTAAGAGCTGTTAATGAGAGCAACTGTGGAAGAGAACATATTGTGCCGAGCATTTTGCAGCTTGCGTTTTTATTGCTAGAGTCAGCGGATGAGGGAAGTCGTCAAGAGGCTTGCAATCTAAGTGGTTTGTTAGGCATTGAGGAGCTTGCTGTTCAGATGCTGAGAACTCTATTTGAGGCCCATGACATGGCAAGGAATGAGATTATCGAGCAGTGTAAATTTCGCATCCTTTCTTTAAAGCCAGAGCAGAGCATGGCAATCATAAGACTGCTTGGTAGTCTGGTTCAAATTTATCCATACCCCATGCTAGAACATGTTTCCCGTCTGAAGGAATTGTTGGATTATTTTGCTTTCATGCATGGCAAGGTTGCTGCTCATCTTGTTTCTGTTTTATTACCTCTCTTGAAGTTCAGCCGCGATCTTCAGAATTACACAATTTTGGTAATGCGTAAGGCGATGTTTAGACGAGAAGATGCGATTCGTCTTGCAGCAACTGGTGCTGTCATTGACCTAATTCTAGCAGAAAAGCAATCCAAGAGAGATGACACATTACTATTTCAAGAGTCATCTAGCCAAGCCAGTAGCAGCCAGCAATCTGAGATGCGCTACAGTGTGGGGGGATGCCTTTTTCAAGAGCTTAGTGGTTTGCTACAGAGATGTCTTTATCAGCAGGCAAATGTCAAAGAAGTCATGTATCATGGACTTATGAAGCTTGTGTTGGTGGACCCGTCAAGTGGTGGAGCTGTCTTTGATTTTCTGTTACCTCACTTCCTCCACTTCTATAAGGAGGATGAAGACGTTCACCTACGAATCAACTGCTGTGTTAAATCAGAGAGTGGCAAGGTTTATATAGAAGAGCCCTTAGATTGTCTTCTGTCTTGTATCTCTTGGATGCTACTTCTACAACCGCATGGTAAAACTGAGCACTTGGAAAATTCATGGGCATGTTTGGGTTTCTCCCTTTCACAAGAAAACGAGGGAGGAAGAAATTTGTCAGGTGATTCGTTCTATACTGCTTTCTTGAAGATACGGAAGTCGTTAAGAAATCAGAGTTTGGAAGGCATCTTTGGTCAATCTCAGGATTCCGGTTCTTCATCTTTTGAAGAGGACAAAAGAAAATGCTGCGCATTTGTTTTATCAGGAATTATTGAAGTAGTCTTGAATGCTATTGCTACTGAGTTGGAGAAATCAACAGACAGAAAGAAGTTGGATTTGGAAAAGGAGCTTATTGAGTTTGTTGACCTTCATGATTCATTGGATAAGGATGCAGGTATCTCCAAGCAGAGTACAACTATCAGAAGAGGGAATCCTCGCACTACTGCTCACGATATGACCAACAACACGGATTCAGGCCAATCAAAAGTGACACAAGGACGATTATCTTTCTTTGCAACTTCAAGTATATATCAGATCTTGCAAACAGTATTGAAACTACTCAATACTGATAATTCTGATAATGTTGCGACTTCCCAAAACCATAGCCAGTTGACTAGCAAAACAACAAAATGCTCTGCTAAGATGATTTGTTTTGTGTTGAATGCATCCCTCAATCATATAAAATCTTCCTCTGTTCTGCGAAATGAAGATCCATTGAGAACTTTGATCTATGGGGAACTTAAGATGCTAGGACCCCCCTTGCTGAAGTTGATCATTTTGCTCAAATCGGGGCCAAAGATGTCAGATCAAAAGAAAAAGGAAGCTAAAGGAAAGAAAGATGTTCATGGACAAAGGGACCATCTTCATCTAGCCTTGATTTGCTTGAAGGAGTTGATAATGATTTGTTCATCAACTTCACACTTGACTGGTTTGCTTGAGGATATGGTGTCAATCTCCACTCTTCAGCATGCTGATTCAAATAATGAAAGTGAAACAATCTTGATAATTGACGATCAACATTTACGAAGCAAAGAACTGTTCATTGTCAAAACCTTGAAGCCTCTATTTTCTGAGCTTCTGGCACTTTCATTCTTCAGTGAAGTTGAGATCATCTCTGACATGATAATGATCATCGGGTGCAAATTGCCATGTGAACTGAGTAACTCCCATGGAGCTTGGGCTGTTAGTGTTTGCAAGAGCAATGAAATATTAAGCTCCAAGGTCGGAAAAAGCATTGTGAGGCTTGCTATATGTTTAAGCTCCCCACCAGATGATTTAATTATATCTCAAGATCTGGCTAGAGAGCTGTTACAAGTTATTGGATCAGAGGGAAATGAACCTGTGAAGGTGTCTGAAGCGTACCCGGTCATAAACCATCTGACAAGCGCCGTATTAAATTCTTGTGTACTGCACACAATTGAAGAAGTTATAGCTGATATGGATTGGGCCACCAAAAAGTTGAAGACATTCTCTTTAGTCTATCAAAAAATGGTTCATTTCAGTCAGAACAACGAGCAATCTTCTGAGTTGGCATTTGAGGAAAATCTTTACCCTAGGGCGGGAGCAGTGGTGAAAGTACTATCATCCTTTGTTTCAATGAACCTCAATGATACTCAAGCTGAGATATTGCTGCGATTGGCTGCGAGGTTTTACAAGCATTTAGCTCAGATGTCAAAGCTTAGGATTGCTCCTAAAGGTTGCAAACAGATCTTTCCTAGCCTCAAGTTTCAGAAGCTTGTGGAAGTAACCTGTAAACAGCTGACGAACCCGTTGTATGTCTTTGTGGACATTATGCAAAAGAAACAACAAGAAAATGCTAACAAAAAGGGGATCATCAACAAAATAAAGAGGGAGAACAGATGCATCCCAGACCTGATATATCAGATAGAAGACTACGAAAAGTATCTCATCCAGCTTAGCAAGGTGTGCAAAGTGAATCTATTAAAGCACGCCAAGCGAAGCACTGCACGCGATTTCAGAATATTCCCGAAAGAGAATGCCACTCCAAGTGATGAAGCAAACCATGCCAACTCCAATGCAGTTGAAAACGAGTCAGCTGAGGATCTCAATGCCAATGACAACGAAGGCGACGATGACGACAGGTTGGAGAAGATTTTATCAGCAGAATCGAATAGTCCTATGGTTGCAGAGGATTCTGGCTCGGAGAATGAAGAAGTGGATGGCCTTCCTAGTCCAAAGAGGTTGAAGAGAAACAACAGAGTTGTGGAGGACTCTGATGATGAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
FANCI_HD1 FANCI_HD2 FANCI_S1 FANCI_S2 FANCI_S4

Protein Analysis

1410

Amino Acids

157.78

Weight (kDa)

6.31

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FANCI_S1 PF14675 121 - 284 1.1e-17 FANCI solenoid 1
FANCI_HD1 PF14679 289 - 369 9.5e-21 FANCI helical domain 1
FANCI_S2 PF14676 382 - 536 4.8e-46 FANCI solenoid 2
FANCI_HD2 PF14680 555 - 790 1.3e-56 FANCI helical domain 2
FANCI_S4 PF14678 1090 - 1321 1.5e-71 FANCI solenoid 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
Acc16I TGCGCA 1 cut(s) 2230
Acc36I ACCTGC 1 cut(s) 2363
AccB7I CCANNNNNTGG 2 cut(s) 706, 1291
AccI GTMKAC 1 cut(s) 639
AccII CGCG 5 cut(s) 159, 507, 1007, 1521, 3951
AciI CCGC 9 cut(s) 12, 157, 507, 510, 1005, 1190, 1519, 2012, 3539
AclWI GGATC 8 cut(s) 270, 851, 864, 906, 2684, 3310, 3818, 4050
AcsI RAATTY 5 cut(s) 1322, 1392, 2038, 2092, 3376
AcuI CTGAAG 6 cut(s) 1451, 1511, 2765, 2927, 3058, 3354
AcyI GRCGYC 3 cut(s) 318, 716, 782
AfaI GTAC 7 cut(s) 369, 698, 1010, 2392, 3341, 3387, 3558
AfiI CCNNNNNNNGG 8 cut(s) 55, 706, 866, 1291, 1432, 1838, 3538, 3539
AflII CTTAAG 1 cut(s) 2720
AflIII ACRYGT 1 cut(s) 1417
AhdI GACNNNNNGTC 1 cut(s) 685
AjnI CCWGG 1 cut(s) 26
AjuI GAANNNNNNNTTGG 8 cut(s) 1424, 1456, 1805, 1837, 2154, 2186, 2300, 2332
Alw21I GWGCWC 2 cut(s) 974, 2031
Alw26I GTCTC 3 cut(s) 193, 208, 234
AlwI GGATC 8 cut(s) 270, 851, 864, 906, 2684, 3310, 3818, 4050
AlwNI CAGNNNCTG 4 cut(s) 706, 929, 1264, 1598
AoxI GGCC 7 cut(s) 182, 1098, 1281, 2455, 2771, 3426, 4162
ApeKI GCWGC 9 cut(s) 703, 997, 1162, 1475, 1592, 1689, 2226, 3610, 3620
ApoI RAATTY 5 cut(s) 1322, 1392, 2038, 2092, 3376
ArsI GACNNNNNNTTYG 4 cut(s) 632, 664, 767, 799
Asp700I GAANNNNTTC 2 cut(s) 1902, 3446
AspA2I CCTAGG 1 cut(s) 3533
AspLEI GCGC 5 cut(s) 161, 997, 1710, 2231, 3368
AspS9I GGNCC 6 cut(s) 1282, 1828, 2732, 2771, 2836, 3426
AsuC2I CCSGG 2 cut(s) 466, 3344
AvaII GGWCC 3 cut(s) 1828, 2732, 2836
AvrII CCTAGG 1 cut(s) 3533
BaeI ACNNNNGTAYC 2 cut(s) 2123, 2156
BamHI GGATCC 1 cut(s) 856
BanII GRGCYC 2 cut(s) 974, 1965
BbsI GAAGAC 4 cut(s) 1905, 1967, 3449, 3876
Bbv12I GWGCWC 2 cut(s) 974, 2031
BbvCI CCTCAGC 2 cut(s) 492, 4038
BbvI GCAGC 9 cut(s) 690, 984, 1174, 1462, 1604, 1701, 2213, 3597, 3607
BccI CCATC 8 cut(s) 344, 380, 574, 874, 970, 2847, 3363, 4154
BceAI ACGGC 3 cut(s) 160, 670, 3353
BcgI CGANNNNNNTGC 4 cut(s) 1141, 1175, 1902, 1936
BciT130I CCWGG 1 cut(s) 28
BciVI GTATCC 1 cut(s) 1038
BclI TGATCA 3 cut(s) 573, 2751, 3108
BcnI CCSGG 2 cut(s) 466, 3344
BcoDI GTCTC 3 cut(s) 193, 208, 234
BfmI CTRYAG 3 cut(s) 1073, 1711, 1754
BfoI RGCGCY 1 cut(s) 3369
BfrI CTTAAG 1 cut(s) 2720
BfuAI ACCTGC 1 cut(s) 2363
BfuI GTATCC 1 cut(s) 1038
BglI GCCNNNNNGGC 1 cut(s) 181
BglII AGATCT 3 cut(s) 2514, 3271, 3687
BlnI CCTAGG 1 cut(s) 3533
BlpI GCTNAGC 1 cut(s) 3896
BmcAI AGTACT 1 cut(s) 3558
Bme1390I CCNGG 3 cut(s) 28, 466, 3344
Bme18I GGWCC 3 cut(s) 1828, 2732, 2836
BmeRI GACNNNNNGTC 1 cut(s) 685
BmgT120I GGNCC 6 cut(s) 1282, 1828, 2732, 2771, 2836, 3426
BmiI GGNNCC 5 cut(s) 858, 1830, 2734, 2772, 2837
BmrFI CCNGG 3 cut(s) 28, 466, 3344
BmrI ACTGGG 1 cut(s) 76
BmuI ACTGGG 1 cut(s) 76
BpiI GAAGAC 4 cut(s) 1905, 1967, 3449, 3876
BplI GAGNNNNNCTC 2 cut(s) 3077, 3109
BpmI CTGGAG 1 cut(s) 566
Bpu10I CCTNAGC 3 cut(s) 492, 3659, 4038
Bpu1102I GCTNAGC 1 cut(s) 3896
BpuEI CTTGAG 4 cut(s) 2936, 3252, 3579, 3686
BpuMI CCSGG 2 cut(s) 466, 3344
BsaAI YACGTR 1 cut(s) 367
BsaBI GATNNNNATC 1 cut(s) 3107
BsaHI GRCGYC 3 cut(s) 318, 716, 782
BsaJI CCNNGG 5 cut(s) 27, 464, 3148, 3198, 3533
BsaWI WCCGGW 2 cut(s) 604, 2189
BsaXI ACNNNNNCTCC 6 cut(s) 98, 128, 308, 338, 3534, 3564
Bsc4I CCNNNNNNNGG 8 cut(s) 55, 706, 866, 1291, 1432, 1838, 3538, 3539
Bse1I ACTGG 6 cut(s) 82, 185, 1603, 1683, 2587, 2911
Bse3DI GCAATG 1 cut(s) 3187
Bse8I GATNNNNATC 1 cut(s) 3107
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 5 cut(s) 27, 464, 3148, 3198, 3533
BseJI GATNNNNATC 1 cut(s) 3107
BseLI CCNNNNNNNGG 8 cut(s) 55, 706, 866, 1291, 1432, 1838, 3538, 3539
BseMI GCAATG 1 cut(s) 3187
BseNI ACTGG 6 cut(s) 82, 185, 1603, 1683, 2587, 2911
BseRI GAGGAG 7 cut(s) 128, 200, 248, 253, 260, 509, 1259
BseXI GCAGC 9 cut(s) 690, 984, 1174, 1462, 1604, 1701, 2213, 3597, 3607
BsgI GTGCAG 2 cut(s) 3374, 3930
Bsh1236I CGCG 5 cut(s) 159, 507, 1007, 1521, 3951
BshFI GGCC 7 cut(s) 184, 1100, 1283, 2457, 2773, 3428, 4164
BsiHKAI GWGCWC 2 cut(s) 974, 2031
BsiSI CCGG 4 cut(s) 466, 605, 2190, 3344
BslFI GGGAC 5 cut(s) 109, 611, 832, 897, 2849
BslI CCNNNNNNNGG 8 cut(s) 55, 706, 866, 1291, 1432, 1838, 3538, 3539
BsmAI GTCTC 3 cut(s) 193, 208, 234
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 5 cut(s) 109, 611, 832, 897, 2849
BsmI GAATGC 3 cut(s) 2272, 2647, 3982
BsnI GGCC 7 cut(s) 184, 1100, 1283, 2457, 2773, 3428, 4164
Bsp1286I GDGCHC 3 cut(s) 974, 1965, 2031
Bsp1720I GCTNAGC 1 cut(s) 3896
Bsp19I CCATGG 1 cut(s) 3148
BspACI CCGC 9 cut(s) 12, 157, 507, 510, 1005, 1190, 1519, 2012, 3539
BspANI GGCC 7 cut(s) 184, 1100, 1283, 2457, 2773, 3428, 4164
BspFNI CGCG 5 cut(s) 159, 507, 1007, 1521, 3951
BspHI TCATGA 1 cut(s) 2350
BspLI GGNNCC 5 cut(s) 858, 1830, 2734, 2772, 2837
BspMAI CTGCAG 1 cut(s) 1077
BspMI ACCTGC 1 cut(s) 2363
BspPI GGATC 8 cut(s) 270, 851, 864, 906, 2684, 3310, 3818, 4050
BspQI GCTCTTC 1 cut(s) 1953
BspTI CTTAAG 1 cut(s) 2720
BsrDI GCAATG 1 cut(s) 3187
BsrI ACTGG 6 cut(s) 82, 185, 1603, 1683, 2587, 2911
BssECI CCNNGG 5 cut(s) 27, 464, 3148, 3198, 3533
BssNI GRCGYC 3 cut(s) 318, 716, 782
BssT1I CCWWGG 3 cut(s) 3148, 3198, 3533
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 8 cut(s) 202, 823, 925, 1013, 1130, 1715, 2527, 3018
Bst6I CTCTTC 6 cut(s) 1129, 1953, 2202, 2398, 2946, 4181
BstACI GRCGYC 3 cut(s) 318, 716, 782
BstAFI CTTAAG 1 cut(s) 2720
BstAPI GCANNNNNTGC 4 cut(s) 682, 1601, 3906, 4004
BstBAI YACGTR 1 cut(s) 367
BstDSI CCRYGG 1 cut(s) 3148
BstENI CCTNNNNNAGG 1 cut(s) 864
BstFNI CGCG 5 cut(s) 159, 507, 1007, 1521, 3951
BstH2I RGCGCY 1 cut(s) 3369
BstHHI GCGC 5 cut(s) 161, 997, 1710, 2231, 3368
BstMAI GTCTC 3 cut(s) 193, 208, 234
BstMWI GCNNNNNNNGC 8 cut(s) 181, 682, 788, 1601, 3906, 3934, 3948, 4004
BstNI CCWGG 1 cut(s) 28
BstNSI RCATGY 3 cut(s) 1421, 2052, 2951
BstSCI CCNGG 3 cut(s) 26, 464, 3342
BstSFI CTRYAG 3 cut(s) 1073, 1711, 1754
BstUI CGCG 5 cut(s) 159, 507, 1007, 1521, 3951
BstV1I GCAGC 9 cut(s) 690, 984, 1174, 1462, 1604, 1701, 2213, 3597, 3607
BstV2I GAAGAC 4 cut(s) 1905, 1967, 3449, 3876
BstX2I RGATCY 7 cut(s) 856, 898, 2514, 2689, 3271, 3687, 4042
BstYI RGATCY 7 cut(s) 856, 898, 2514, 2689, 3271, 3687, 4042
BsuI GTATCC 1 cut(s) 1038
BsuRI GGCC 7 cut(s) 184, 1100, 1283, 2457, 2773, 3428, 4164
BtgI CCRYGG 1 cut(s) 3148
BtgZI GCGATG 2 cut(s) 205, 1574
BtsI GCAGTG 3 cut(s) 1322, 3552, 3942
BtsIMutI CAGTG 5 cut(s) 1322, 1720, 3082, 3552, 3942
BveI ACCTGC 1 cut(s) 2363
CaiI CAGNNNCTG 4 cut(s) 706, 929, 1264, 1598
CciI TCATGA 1 cut(s) 2350
CfoI GCGC 5 cut(s) 161, 997, 1710, 2231, 3368
Cfr13I GGNCC 6 cut(s) 1282, 1828, 2732, 2771, 2836, 3426
CseI GACGC 4 cut(s) 111, 326, 705, 771
Csp6I GTAC 7 cut(s) 368, 697, 1009, 2391, 3340, 3386, 3557
CspCI CAANNNNNGTGG 2 cut(s) 1207, 1242
CviQI GTAC 7 cut(s) 368, 697, 1009, 2391, 3340, 3386, 3557
DraI TTTAAA 1 cut(s) 1341
DrdI GACNNNNNNGTC 1 cut(s) 200
DriI GACNNNNNGTC 1 cut(s) 685
DseDI GACNNNNNNGTC 1 cut(s) 200
Eam1104I CTCTTC 6 cut(s) 1129, 1953, 2202, 2398, 2946, 4181
Eam1105I GACNNNNNGTC 1 cut(s) 685
EarI CTCTTC 6 cut(s) 1129, 1953, 2202, 2398, 2946, 4181
Ecl136II GAGCTC 1 cut(s) 972
Eco130I CCWWGG 3 cut(s) 3148, 3198, 3533
Eco24I GRGCYC 2 cut(s) 974, 1965
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 3 cut(s) 1828, 2732, 2836
Eco53kI GAGCTC 1 cut(s) 972
Eco57I CTGAAG 6 cut(s) 1451, 1511, 2765, 2927, 3058, 3354
EcoICRI GAGCTC 1 cut(s) 972
EcoNI CCTNNNNNAGG 1 cut(s) 864
EcoO109I RGGNCCY 1 cut(s) 2732
EcoRII CCWGG 1 cut(s) 26
EcoRV GATATC 1 cut(s) 67
EcoT14I CCWWGG 3 cut(s) 3148, 3198, 3533
EcoT22I ATGCAT 3 cut(s) 1464, 2647, 3845
EcoT38I GRGCYC 2 cut(s) 974, 1965
ErhI CCWWGG 3 cut(s) 3148, 3198, 3533
Esp3I CGTCTC 1 cut(s) 208
FaqI GGGAC 5 cut(s) 109, 611, 832, 897, 2849
FauI CCCGC 3 cut(s) 164, 503, 3532
FbaI TGATCA 3 cut(s) 573, 2751, 3108
FblI GTMKAC 1 cut(s) 639
FriOI GRGCYC 2 cut(s) 974, 1965
FspI TGCGCA 1 cut(s) 2230
GlaI GCGC 5 cut(s) 160, 996, 1709, 2230, 3367
GsuI CTGGAG 1 cut(s) 566
HaeII RGCGCY 1 cut(s) 3369
HaeIII GGCC 7 cut(s) 184, 1100, 1283, 2457, 2773, 3428, 4164
HapII CCGG 4 cut(s) 466, 605, 2190, 3344
HgaI GACGC 4 cut(s) 111, 326, 705, 771
HhaI GCGC 5 cut(s) 161, 997, 1710, 2231, 3368
Hin1I GRCGYC 3 cut(s) 318, 716, 782
Hin6I GCGC 5 cut(s) 159, 995, 1708, 2229, 3366
HinP1I GCGC 5 cut(s) 159, 995, 1708, 2229, 3366
HincII GTYRAC 2 cut(s) 2344, 2592
HindII GTYRAC 2 cut(s) 2344, 2592
HindIII AAGCTT 3 cut(s) 1814, 3656, 3713
HpaII CCGG 4 cut(s) 466, 605, 2190, 3344
Hpy99I CGWCG 5 cut(s) 181, 320, 431, 691, 4073
HpyCH4III ACNGT 8 cut(s) 202, 823, 925, 1013, 1130, 1715, 2527, 3018
HpyCH4IV ACGT 2 cut(s) 366, 1902
HpyF10VI GCNNNNNNNGC 8 cut(s) 181, 682, 788, 1601, 3906, 3934, 3948, 4004
HpySE526I ACGT 2 cut(s) 366, 1902
Hsp92I GRCGYC 3 cut(s) 318, 716, 782
HspAI GCGC 5 cut(s) 159, 995, 1708, 2229, 3366
Ksp22I TGATCA 3 cut(s) 573, 2751, 3108
LguI GCTCTTC 1 cut(s) 1953
LmnI GCTCC 9 cut(s) 95, 1246, 1844, 2326, 3152, 3200, 3245, 3542, 3673
Lsp1109I GCAGC 9 cut(s) 690, 984, 1174, 1462, 1604, 1701, 2213, 3597, 3607
MaeII ACGT 2 cut(s) 366, 1902
MaeIII GTNAC 5 cut(s) 1863, 2467, 3140, 3288, 3724
MfeI CAATTG 1 cut(s) 3396
MflI RGATCY 7 cut(s) 856, 898, 2514, 2689, 3271, 3687, 4042
MhlI GDGCHC 3 cut(s) 974, 1965, 2031
MlyI GAGTC 4 cut(s) 1193, 1676, 4040, 4205
MmeI TCCRAC 7 cut(s) 216, 876, 1422, 2265, 2292, 3184, 4065
Mph1103I ATGCAT 3 cut(s) 1464, 2647, 3845
MroXI GAANNNNTTC 2 cut(s) 1902, 3446
MslI CAYNNNNRTG 2 cut(s) 2047, 3104
MspA1I CMGCKG 4 cut(s) 703, 1190, 3739, 4037
MspCI CTTAAG 1 cut(s) 2720
MspI CCGG 4 cut(s) 466, 605, 2190, 3344
MspR9I CCNGG 3 cut(s) 28, 466, 3344
MunI CAATTG 1 cut(s) 3396
Mva1269I GAATGC 3 cut(s) 2272, 2647, 3982
MvaI CCWGG 1 cut(s) 28
MvnI CGCG 5 cut(s) 159, 507, 1007, 1521, 3951
MwoI GCNNNNNNNGC 8 cut(s) 181, 682, 788, 1601, 3906, 3934, 3948, 4004
NciI CCSGG 2 cut(s) 466, 3344
NcoI CCATGG 1 cut(s) 3148
NlaIV GGNNCC 5 cut(s) 858, 1830, 2734, 2772, 2837
NmeAIII GCCGAG 1 cut(s) 1176
NmuCI GTSAC 1 cut(s) 2467
NsbI TGCGCA 1 cut(s) 2230
NsiI ATGCAT 3 cut(s) 1464, 2647, 3845
NspI RCATGY 3 cut(s) 1421, 2052, 2951
PaeI GCATGC 1 cut(s) 2951
PagI TCATGA 1 cut(s) 2350
PciI ACATGT 1 cut(s) 1417
PciSI GCTCTTC 1 cut(s) 1953
PcsI WCGNNNNNNNCGW 2 cut(s) 779, 4068
PctI GAATGC 3 cut(s) 2272, 2647, 3982
PdmI GAANNNNTTC 2 cut(s) 1902, 3446
PflMI CCANNNNNTGG 2 cut(s) 706, 1291
PleI GAGTC 4 cut(s) 1192, 1675, 4039, 4205
PpsI GAGTC 4 cut(s) 1192, 1675, 4039, 4205
Ppu21I YACGTR 1 cut(s) 367
PpuMI RGGWCCY 1 cut(s) 2732
PscI ACATGT 1 cut(s) 1417
Psp124BI GAGCTC 1 cut(s) 974
Psp5II RGGWCCY 1 cut(s) 2732
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 5 cut(s) 858, 1830, 2734, 2772, 2837
PspPI GGNCC 6 cut(s) 1282, 1828, 2732, 2771, 2836, 3426
PspPPI RGGWCCY 1 cut(s) 2732
PstI CTGCAG 1 cut(s) 1077
PstNI CAGNNNCTG 4 cut(s) 706, 929, 1264, 1598
PsuI RGATCY 7 cut(s) 856, 898, 2514, 2689, 3271, 3687, 4042
PvuII CAGCTG 3 cut(s) 703, 3739, 4037
RsaI GTAC 7 cut(s) 369, 698, 1010, 2392, 3341, 3387, 3558
RsaNI GTAC 7 cut(s) 368, 697, 1009, 2391, 3340, 3386, 3557
RseI CAYNNNNRTG 2 cut(s) 2047, 3104
SacI GAGCTC 1 cut(s) 974
SapI GCTCTTC 1 cut(s) 1953
Sau96I GGNCC 6 cut(s) 1282, 1828, 2732, 2771, 2836, 3426
ScaI AGTACT 1 cut(s) 3558
SchI GAGTC 4 cut(s) 1193, 1676, 4040, 4205
ScrFI CCNGG 3 cut(s) 28, 466, 3344
SduI GDGCHC 3 cut(s) 974, 1965, 2031
SfcI CTRYAG 3 cut(s) 1073, 1711, 1754
SinI GGWCC 3 cut(s) 1828, 2732, 2836
SmiMI CAYNNNNRTG 2 cut(s) 2047, 3104
SmlI CTYRAG 5 cut(s) 2720, 2915, 3267, 3594, 3701
SmoI CTYRAG 5 cut(s) 2720, 2915, 3267, 3594, 3701
SphI GCATGC 1 cut(s) 2951
SsiI CCGC 9 cut(s) 12, 157, 507, 510, 1005, 1190, 1519, 2012, 3539
SspI AATATT 2 cut(s) 3189, 3963
SstI GAGCTC 1 cut(s) 974
StyD4I CCNGG 3 cut(s) 26, 464, 3342
StyI CCWWGG 3 cut(s) 3148, 3198, 3533
TaaI ACNGT 8 cut(s) 202, 823, 925, 1013, 1130, 1715, 2527, 3018
TaiI ACGT 2 cut(s) 369, 1905
TatI WGTACW 3 cut(s) 2390, 3385, 3556
TauI GCSGC 2 cut(s) 510, 1521
TscAI CASTG 5 cut(s) 1322, 1720, 3082, 3552, 3949
TseFI GTSAC 1 cut(s) 2467
TseI GCWGC 9 cut(s) 703, 997, 1162, 1475, 1592, 1689, 2226, 3610, 3620
Tsp45I GTSAC 1 cut(s) 2467
TspGWI ACGGA 2 cut(s) 2149, 2462
TspRI CASTG 5 cut(s) 1322, 1720, 3082, 3552, 3949
Van91I CCANNNNNTGG 2 cut(s) 706, 1291
Vha464I CTTAAG 1 cut(s) 2720
VpaK11BI GGWCC 3 cut(s) 1828, 2732, 2836
XagI CCTNNNNNAGG 1 cut(s) 864
XapI RAATTY 5 cut(s) 1322, 1392, 2038, 2092, 3376
XbaI TCTAGA 1 cut(s) 610
XceI RCATGY 3 cut(s) 1421, 2052, 2951
XmaJI CCTAGG 1 cut(s) 3533
XmiI GTMKAC 1 cut(s) 639
XmnI GAANNNNTTC 2 cut(s) 1902, 3446
ZrmI AGTACT 1 cut(s) 3558
Zsp2I ATGCAT 3 cut(s) 1464, 2647, 3845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.