FvH4_4g18510

FANCI solenoid 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009455
Physical Location & Seq
Forward (+)
22356233 .. 22363346
7114 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18510.t1

Sequence Viewer

Length: 3666 bp
ATGGTTTCGATAGTGAGGGACTTCCCTGTTCTTGATAAACTGCGGGCCAGAGAGTTTGTAGACAAGGTGTTCGACGGAATGTCTAGCTTGGACTTGCAGGACCTGCCGTCGCTTGTGTACCAGTTGCTGGTTCTGGCGTCGAGGGGGTTTGTTAAGAAGGATGTGGTTGAAGGGATTGTGATGTTTTTCGGGGCGAAAATGGGGAGGAAGAAGGCGAGTTCTATTGTTAGGCAAGTGGAAGGGACTGTGTTGCTTCATGTTAACTTTGCGGTGAAGCAGGACCCTTCGTTGGGACAGGAGGTGATGGGGCTTGTGAAGTCTGATCTCAGGGCTTTCAATCATTTCACTGTTGCTGTTTTATTATCGGTTTCGAGGGTGAGGAAGTTTGGTGAGGCGTCAATGGGGGCTCTGAAAATGGCGATTTTAACTGCGTATCGTGACTACAAGTTTGCCAAAGACTGTAAATGGATACCCGATGACATGAAGGAAGAATATCTGCAGAATGTCAAAGTGGTCGAGAAGGCCATGTTAAGAGCGGTTCACGAGAGCAACTATGGAAGGGAACACATTGTTCCTAGCATTTTGCAGTTTGCTTTTGTATTGCTAGAGTCAGTGGAAGAGGGGAATTATAAAGAGCTCTGCAATTTAAGTGGTGTGTTAGGCATTGAGGAGCTTGCTATTCAGATGCTAAAAGCTATATTTGTGGCCCATGATATGGCTAGAAATGAGATTATTGAGCAGTGTAAATTTCGGATCCTTTCTTTGAAGCCAGAGCAGAGTATGGTAATCATAAGACTACTCAGTAATCTCACTCAAAGTTATCCATACCCCATGTTAGAACATGTTTCCCGTCTAAAGGAACTGTTGGACTACTTCACATTCATGCATGGCAAGGTTGCTGCCCAACTTGTTACTGCTTTGTTGCCTCTCATCAAGTTCAGCCGTGATCTCAAGGATTACACTATTTTGGTAATGCGTAAGGCGATGTTTAGACGAGAAGATACTATTCGTCTTGCAGCAACCAATGCTGTTATTGATTTGATTCTGGCAGAAAAGCAATCCAAGAAAGATGATTTGTTGTCTTTTCAAGAGTCATCTAGCCAAGCCAGTAGTAGTCAGCAATCTGAGGTACCCTACAATGTGAGGGGAGGCCTGTTTCAAGAGCTGAGCGGTCTATTGCAGAGATGTCTATATCAACCGGCAAATGTCAAAGAAGTCATGTATCATGGACTTGTGAAGCTTGTTTTGATGGACCCATCTAGTGGAGGGGCTGTCTTTGATTTTCTGTTGCCTCACTTCCTCCATTTCTTTAAGGAGGATGCAGATGTTCAATTAGGAATTAGCTGCTGCATAAAATCAGAGAGTGGCAAAGCTTATGTTGAAGAGCCTTTAGATTGCCTTCTATCTTGTGTTTCTCGGATGTTACTTCTACAACCAAATGGAAAAACTGAGAAAGCCTTGAATTCTTCATGGGCATGTTTGGGTTTCTCCCTTTCCCAAGAAAACGAGCTAGGAAGAAGTTCATCTGCTGACACATTCTACAGTTCTATCTTGAAGATACGGAAGTCTCTTCGAAATCAGAATATTGAAGACATCTTTGGTCAAACTCAGGATGGAGGCTCGTCATCTTTTGAAGACAACAAAAGAAAATGCTCCGCTTTTGTTTTATCTGGAATTATTGAGGTACTGGTGAATGCTATTGCTACTGACTTGGACAAAGCATCGGACAGAAAGAAATTAGATCTTGAAAAGGAGATTATTCAGTTTGTTGAGATTCATCATTCACTTGACAAGGATACATGTTTCTCCAGCAAGAGTACTGCAGTAAGAAAAGGGAGTACTCGCACTCCTGCTCATGATATACTGAACAACATCAATTCAAGCCCTAAAGAAATGCAAGCACGGGTTTCCTTCTTTACAACTTCAACCATATATCAGATATTGCAAACACTGTTGAGACTACTAGATACTGATTCTTCTCATAATGTTGAAGCTTCCCAACTGCATAGTCAGTCTACCAGCAAGACCTCCAAATGCAGCTCTGAATTTATTTCTTTTGTCTTGAATGAGTCCCTCAGTCATATAAAATCTTCCATTCTTCTGCGAAATGAAGACCCTTTGAGAACTTTAATCTATGGGGAAATCAAGATGCTGGGATCTCCCTTGTTGAAACTAATTTCTTTGTTCAAATCAGGGCCAAAGTTGGTGATGGATCAAAAGAAAAAGGAAACGAAGGGCAAAAAAGATGTTGGACGAAGGGAGTATCTTCATCTAGCCTTGAGTTGCTTGAAGGAATTGATAATGATTTGTTCTAATTCAGACTTGACTGGTTTGCTTGAAGACATGGTGTTAATTTCCACTCTTAAGGATGCTGATTTGAATATTGAATGCGAAAGAGCCTCTAGAATTGAGGATCCACATATAAGAAGCATAGAACTCTTCATTTTGAAGACATTGAAGCCTCTATTCTCTGAGCTTCTTGCACTTTCATTATTTGGTGAAGTTGAGATAATTTGTGACCTGATATTGATGATTGGGCGGAAATTGCCATGCCAATTGAGGAACTCCCATGGAGCTTGGGCTATTAGTGTTTGCAAAAACAATGAAATTAAAAACACAAAGGCCGGAAAAAGTGTTGTGACACTTGCTATATGTTTAAGTTCCCCTCCAAATGATCTAGCTATATCCCAAGACATGGCCAGAGAGCTGTTGCAGGTTATTGGATCAGAGGGAGACGCCCCAGAGGAGGTTTCCCAGATATACCCCACCATAAACCATCAAACATGTTCGGTGATAAGTTCCTGTATACTGCACATTGTTGAATCAGTTGTAGTTGACATGGATTGGGCCATCAAAAAACTGAAGACATTCTCTTTGGTCTCCCAAAAAGGGATTCATCTGAACCAGAACAGTGAGGAATCTCCTGAGTTGGCATTTGAGGAAAATCTCTATTCTAGGGCAGAAGCAGTGGTGAAAGTACTGTCATCTTTTGTTTCAATGAGCCTGAATGATACACAAGCTGAATTTGTGCTTCGTTTGGCAGCAAGGTTCTACAAGCATTTAGCTAAGATGTCAAAGCTTAGGATTGCTCCAAAAGGTTGTCAACAGATTTTTCCAAGCCTGAAGTTTCAGAAGCTTGTGGAGGTAACTTGCAAGGAGCTGACAATTCCTCTGTATATCTTTGTGGAAATTATGCAGAAGAAACAACAAGCCAATGCCAACAAAAAGGGAATCATCAACAGAATAAAGAGGGAGAACAGATGCATCCCCGACCTCATATTTCAGATAGAAGATTATGAGAAATATCTCATCCAGCTGTGCAAGGTGACCAAAGTAAATTTATTAAGGCATGCCAAGCGTAGCACTGCACGAGACTTTAGAATACAACTAAATGAGGAGAGCAGGGAAGAAGAAGAAGAAGAAAATGCTCCAACTTCTGAAGCAAACCATGACAATGAGAATGCAGTTGAAAATGAGTCTGCTGAAGATCTTGATGATAATGAAGGAAATGGGTCAGAGAGGGCTTTGTCACCGGATTATATTAACAATCCCCCGGCTGTGGAGGATTCGGGCTCTGACAATGAAGAAGGAGATGATCATCCTCCTAGTGCCAAGAGGCTGAAGAGAGTCAACAGAGTTGTGGAGGACTCTGAGGATGAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
FANCI_HD1 FANCI_HD2 FANCI_S1 FANCI_S2 FANCI_S4

Protein Analysis

1222

Amino Acids

137.36

Weight (kDa)

6.14

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FANCI_S1 PF14675 11 - 92 1.2e-08 FANCI solenoid 1
FANCI_HD1 PF14679 96 - 177 2.8e-22 FANCI helical domain 1
FANCI_S2 PF14676 190 - 344 2.1e-49 FANCI solenoid 2
FANCI_HD2 PF14680 363 - 600 5.5e-56 FANCI helical domain 2
FANCI_S4 PF14678 897 - 1131 2.8e-71 FANCI solenoid 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
Acc36I ACCTGC 2 cut(s) 111, 2704
Acc65I GGTACC 1 cut(s) 1129
AccB1I GGYRCC 1 cut(s) 1129
AccB7I CCANNNNNTGG 4 cut(s) 127, 715, 1262, 2695
AccBSI CCGCTC 2 cut(s) 536, 1170
AccI GTMKAC 3 cut(s) 60, 2015, 2806
AciI CCGC 6 cut(s) 43, 269, 536, 1170, 1656, 2539
AclWI GGATC 7 cut(s) 748, 761, 2164, 2220, 2408, 2421, 2731
AcoI YGGCCR 1 cut(s) 2697
AcsI RAATTY 5 cut(s) 746, 1462, 2045, 3023, 3337
AcuI CTGAAG 5 cut(s) 2882, 3143, 3459, 3504, 3641
AcyI GRCGYC 3 cut(s) 137, 395, 2736
AfaI GTAC 6 cut(s) 119, 1131, 1686, 1819, 1840, 2979
AflII CTTAAG 1 cut(s) 2363
AflIII ACRYGT 3 cut(s) 841, 1799, 2783
AhdI GACNNNNNGTC 2 cut(s) 79, 106
AjuI GAANNNNNNNTTGG 4 cut(s) 1581, 1613, 2877, 2909
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 5 cut(s) 1572, 1951, 2727, 2884, 3366
AlwI GGATC 7 cut(s) 748, 761, 2164, 2220, 2408, 2421, 2731
AlwNI CAGNNNCTG 2 cut(s) 103, 127
AoxI GGCC 8 cut(s) 45, 522, 705, 1150, 2195, 2622, 2697, 2847
ApeKI GCWGC 6 cut(s) 899, 1016, 1344, 1347, 2037, 3041
ApoI RAATTY 5 cut(s) 746, 1462, 2045, 3023, 3337
ArsI GACNNNNNNTTYG 6 cut(s) 53, 85, 431, 463, 2857, 2889
Asp700I GAANNNNTTC 2 cut(s) 1519, 2867
Asp718I GGTACC 1 cut(s) 1129
AspS9I GGNCC 7 cut(s) 45, 100, 280, 706, 1252, 2195, 2847
AsuC2I CCSGG 1 cut(s) 3554
AsuII TTCGAA 1 cut(s) 1573
AvaII GGWCC 3 cut(s) 100, 280, 1252
BaeI ACNNNNGTAYC 2 cut(s) 1550, 1583
BalI TGGCCA 1 cut(s) 2699
BamHI GGATCC 2 cut(s) 753, 2413
BanI GGYRCC 1 cut(s) 1129
BanII GRGCYC 3 cut(s) 409, 639, 3575
BauI CACGAG 2 cut(s) 542, 3369
BbsI GAAGAC 6 cut(s) 1596, 1641, 2118, 2346, 2456, 2870
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 6 cut(s) 886, 1028, 1331, 1334, 2049, 3053
BccI CCATC 7 cut(s) 298, 1243, 1264, 1607, 2203, 2784, 2858
BceAI ACGGC 2 cut(s) 91, 927
BciVI GTATCC 2 cut(s) 462, 1789
BclI TGATCA 1 cut(s) 3595
BcnI CCSGG 1 cut(s) 3554
BcoDI GTCTC 5 cut(s) 1572, 1951, 2727, 2884, 3366
BfmI CTRYAG 3 cut(s) 497, 1540, 1821
BfrI CTTAAG 1 cut(s) 2363
BfuAI ACCTGC 2 cut(s) 111, 2704
BfuI GTATCC 2 cut(s) 462, 1789
BglII AGATCT 2 cut(s) 1741, 3487
BisI GCNGC 6 cut(s) 900, 1017, 1345, 1348, 2038, 3042
BlpI GCTNAGC 1 cut(s) 1166
BlsI GCNGC 6 cut(s) 901, 1018, 1346, 1349, 2039, 3043
BmcAI AGTACT 3 cut(s) 1819, 1840, 2979
Bme1390I CCNGG 1 cut(s) 3554
Bme18I GGWCC 3 cut(s) 100, 280, 1252
BmeRI GACNNNNNGTC 2 cut(s) 79, 106
BmgT120I GGNCC 7 cut(s) 45, 100, 280, 706, 1252, 2195, 2847
BmiI GGNNCC 5 cut(s) 282, 755, 1131, 1254, 2415
BmrFI CCNGG 1 cut(s) 3554
BmsI GCATC 7 cut(s) 675, 1309, 1730, 2139, 2359, 3251, 3273
BpiI GAAGAC 6 cut(s) 1596, 1641, 2118, 2346, 2456, 2870
BpmI CTGGAG 1 cut(s) 1792
Bpu10I CCTNAGC 1 cut(s) 3080
Bpu1102I GCTNAGC 1 cut(s) 1166
Bpu14I TTCGAA 1 cut(s) 1573
BpuEI CTTGAG 2 cut(s) 935, 2299
BpuMI CCSGG 1 cut(s) 3554
BsaBI GATNNNNATC 1 cut(s) 3597
BsaHI GRCGYC 3 cut(s) 137, 395, 2736
BsaI GGTCTC 1 cut(s) 2884
BsaJI CCNNGG 2 cut(s) 2569, 3552
BsaWI WCCGGW 1 cut(s) 3532
BsaXI ACNNNNNCTCC 4 cut(s) 1257, 1287, 1831, 1861
Bse118I RCCGGY 1 cut(s) 1198
Bse1I ACTGG 4 cut(s) 121, 1107, 1692, 2332
Bse8I GATNNNNATC 1 cut(s) 3597
BseDI CCNNGG 2 cut(s) 2569, 3552
BseGI GGATG 9 cut(s) 166, 1324, 1425, 1618, 2374, 3264, 3309, 3598, 3661
BseJI GATNNNNATC 1 cut(s) 3597
BseNI ACTGG 4 cut(s) 121, 1107, 1692, 2332
BseRI GAGGAG 3 cut(s) 683, 2759, 3410
BseXI GCAGC 6 cut(s) 886, 1028, 1331, 1334, 2049, 3053
BseYI CCCAGC 1 cut(s) 2152
BsgI GTGCAG 2 cut(s) 2795, 3351
BshFI GGCC 8 cut(s) 47, 524, 707, 1152, 2197, 2624, 2699, 2849
BshNI GGYRCC 1 cut(s) 1129
BsiHKAI GWGCWC 1 cut(s) 639
BsiSI CCGG 4 cut(s) 1199, 2625, 3533, 3554
BslFI GGGAC 4 cut(s) 32, 256, 306, 2056
BsmAI GTCTC 5 cut(s) 1572, 1951, 2727, 2884, 3366
BsmBI CGTCTC 1 cut(s) 2727
BsmFI GGGAC 4 cut(s) 32, 256, 306, 2056
BsmI GAATGC 3 cut(s) 1699, 2393, 3466
BsnI GGCC 8 cut(s) 47, 524, 707, 1152, 2197, 2624, 2699, 2849
Bso31I GGTCTC 1 cut(s) 2884
Bsp119I TTCGAA 1 cut(s) 1573
Bsp1286I GDGCHC 3 cut(s) 409, 639, 3575
Bsp1720I GCTNAGC 1 cut(s) 1166
Bsp19I CCATGG 1 cut(s) 2569
BspACI CCGC 6 cut(s) 43, 269, 536, 1170, 1656, 2539
BspANI GGCC 8 cut(s) 47, 524, 707, 1152, 2197, 2624, 2699, 2849
BspHI TCATGA 1 cut(s) 1855
BspLI GGNNCC 5 cut(s) 282, 755, 1131, 1254, 2415
BspMAI CTGCAG 2 cut(s) 501, 1825
BspMI ACCTGC 2 cut(s) 111, 2704
BspPI GGATC 7 cut(s) 748, 761, 2164, 2220, 2408, 2421, 2731
BspQI GCTCTTC 1 cut(s) 1377
BspT104I TTCGAA 1 cut(s) 1573
BspT107I GGYRCC 1 cut(s) 1129
BspTI CTTAAG 1 cut(s) 2363
BspTNI GGTCTC 1 cut(s) 2884
BsrBI CCGCTC 2 cut(s) 536, 1170
BsrFI RCCGGY 1 cut(s) 1198
BsrI ACTGG 4 cut(s) 121, 1107, 1692, 2332
BssAI RCCGGY 1 cut(s) 1198
BssECI CCNNGG 2 cut(s) 2569, 3552
BssNAI GTATAC 1 cut(s) 2807
BssNI GRCGYC 3 cut(s) 137, 395, 2736
BssSI CACGAG 2 cut(s) 542, 3369
BssT1I CCWWGG 1 cut(s) 2569
Bst1107I GTATAC 1 cut(s) 2807
Bst2BI CACGAG 2 cut(s) 542, 3369
Bst4CI ACNGT 8 cut(s) 247, 349, 461, 864, 1544, 1953, 2912, 2982
Bst6I CTCTTC 5 cut(s) 612, 1377, 1575, 2444, 3617
BstACI GRCGYC 3 cut(s) 137, 395, 2736
BstAFI CTTAAG 1 cut(s) 2363
BstAPI GCANNNNNTGC 2 cut(s) 103, 1025
BstBI TTCGAA 1 cut(s) 1573
BstC8I GCNNGC 4 cut(s) 45, 675, 1899, 3351
BstDSI CCRYGG 1 cut(s) 2569
BstEII GGTNACC 1 cut(s) 3325
BstF5I GGATG 9 cut(s) 166, 1324, 1425, 1618, 2374, 3264, 3309, 3598, 3661
BstMAI GTCTC 5 cut(s) 1572, 1951, 2727, 2884, 3366
BstMWI GCNNNNNNNGC 4 cut(s) 103, 1025, 2545, 3355
BstNSI RCATGY 5 cut(s) 845, 1479, 1803, 2787, 3353
BstPI GGTNACC 1 cut(s) 3325
BstSCI CCNGG 1 cut(s) 3552
BstSFI CTRYAG 3 cut(s) 497, 1540, 1821
BstV1I GCAGC 6 cut(s) 886, 1028, 1331, 1334, 2049, 3053
BstV2I GAAGAC 6 cut(s) 1596, 1641, 2118, 2346, 2456, 2870
BstX2I RGATCY 5 cut(s) 753, 1741, 2156, 2413, 3487
BstYI RGATCY 5 cut(s) 753, 1741, 2156, 2413, 3487
BstZ17I GTATAC 1 cut(s) 2807
BsuI GTATCC 2 cut(s) 462, 1789
BsuRI GGCC 8 cut(s) 47, 524, 707, 1152, 2197, 2624, 2699, 2849
BtgI CCRYGG 1 cut(s) 2569
BtgZI GCGATG 1 cut(s) 998
BtsCI GGATG 9 cut(s) 166, 1324, 1425, 1618, 2374, 3264, 3309, 3598, 3661
BtsI GCAGTG 3 cut(s) 746, 2973, 3363
BtsIMutI CAGTG 7 cut(s) 345, 618, 746, 1949, 2917, 2973, 3363
BveI ACCTGC 2 cut(s) 111, 2704
Cac8I GCNNGC 4 cut(s) 45, 675, 1899, 3351
CaiI CAGNNNCTG 2 cut(s) 103, 127
CciI TCATGA 1 cut(s) 1855
Cfr10I RCCGGY 1 cut(s) 1198
Cfr13I GGNCC 7 cut(s) 45, 100, 280, 706, 1252, 2195, 2847
CseI GACGC 3 cut(s) 126, 384, 2744
Csp6I GTAC 6 cut(s) 118, 1130, 1685, 1818, 1839, 2978
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviQI GTAC 6 cut(s) 118, 1130, 1685, 1818, 1839, 2978
DriI GACNNNNNGTC 2 cut(s) 79, 106
EaeI YGGCCR 1 cut(s) 2697
Eam1104I CTCTTC 5 cut(s) 612, 1377, 1575, 2444, 3617
Eam1105I GACNNNNNGTC 2 cut(s) 79, 106
EarI CTCTTC 5 cut(s) 612, 1377, 1575, 2444, 3617
EciI GGCGGA 1 cut(s) 2554
Ecl136II GAGCTC 1 cut(s) 637
Eco130I CCWWGG 1 cut(s) 2569
Eco147I AGGCCT 1 cut(s) 1152
Eco24I GRGCYC 3 cut(s) 409, 639, 3575
Eco31I GGTCTC 1 cut(s) 2884
Eco47I GGWCC 3 cut(s) 100, 280, 1252
Eco53kI GAGCTC 1 cut(s) 637
Eco57I CTGAAG 5 cut(s) 2882, 3143, 3459, 3504, 3641
Eco91I GGTNACC 1 cut(s) 3325
EcoICRI GAGCTC 1 cut(s) 637
EcoO109I RGGNCCY 2 cut(s) 100, 280
EcoO65I GGTNACC 1 cut(s) 3325
EcoRI GAATTC 1 cut(s) 1462
EcoT14I CCWWGG 1 cut(s) 2569
EcoT22I ATGCAT 2 cut(s) 888, 3266
EcoT38I GRGCYC 3 cut(s) 409, 639, 3575
ErhI CCWWGG 1 cut(s) 2569
Esp3I CGTCTC 1 cut(s) 2727
FaqI GGGAC 4 cut(s) 32, 256, 306, 2056
FauI CCCGC 1 cut(s) 36
FbaI TGATCA 1 cut(s) 3595
FblI GTMKAC 3 cut(s) 60, 2015, 2806
Fnu4HI GCNGC 6 cut(s) 900, 1017, 1345, 1348, 2038, 3042
FokI GGATG 8 cut(s) 173, 1331, 1432, 1625, 2381, 3251, 3296, 3585
FriOI GRGCYC 3 cut(s) 409, 639, 3575
Fsp4HI GCNGC 6 cut(s) 900, 1017, 1345, 1348, 2038, 3042
GluI GCNGC 6 cut(s) 900, 1017, 1345, 1348, 2038, 3042
GsaI CCCAGC 1 cut(s) 2156
GsuI CTGGAG 1 cut(s) 1792
HaeIII GGCC 8 cut(s) 47, 524, 707, 1152, 2197, 2624, 2699, 2849
HapII CCGG 4 cut(s) 1199, 2625, 3533, 3554
HgaI GACGC 3 cut(s) 126, 384, 2744
Hin1I GRCGYC 3 cut(s) 137, 395, 2736
HincII GTYRAC 4 cut(s) 262, 2836, 3104, 3631
HindII GTYRAC 4 cut(s) 262, 2836, 3104, 3631
HindIII AAGCTT 5 cut(s) 1238, 1371, 1992, 3077, 3134
HpaI GTTAAC 1 cut(s) 262
HpaII CCGG 4 cut(s) 1199, 2625, 3533, 3554
Hpy166II GTNNAC 9 cut(s) 61, 118, 262, 541, 2016, 2807, 2836, 3104, 3631
Hpy8I GTNNAC 9 cut(s) 61, 118, 262, 541, 2016, 2807, 2836, 3104, 3631
Hpy99I CGWCG 3 cut(s) 77, 112, 142
HpyCH4III ACNGT 8 cut(s) 247, 349, 461, 864, 1544, 1953, 2912, 2982
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 1025, 2545, 3355
Hsp92I GRCGYC 3 cut(s) 137, 395, 2736
KpnI GGTACC 1 cut(s) 1133
Ksp22I TGATCA 1 cut(s) 3595
KspAI GTTAAC 1 cut(s) 262
LguI GCTCTTC 1 cut(s) 1377
LmnI GCTCC 6 cut(s) 670, 1658, 2573, 3094, 3157, 3433
Lsp1109I GCAGC 6 cut(s) 886, 1028, 1331, 1334, 2049, 3053
LweI GCATC 7 cut(s) 675, 1309, 1730, 2139, 2359, 3251, 3273
MaeIII GTNAC 8 cut(s) 437, 910, 1422, 2516, 2638, 3145, 3325, 3528
MbiI CCGCTC 2 cut(s) 536, 1170
MfeI CAATTG 1 cut(s) 2555
MflI RGATCY 5 cut(s) 753, 1741, 2156, 2413, 3487
MhlI GDGCHC 3 cut(s) 409, 639, 3575
MlsI TGGCCA 1 cut(s) 2699
MluNI TGGCCA 1 cut(s) 2699
MlyI GAGTC 6 cut(s) 617, 1100, 2078, 3485, 3636, 3641
MmeI TCCRAC 3 cut(s) 846, 2230, 3455
Mox20I TGGCCA 1 cut(s) 2699
Mph1103I ATGCAT 2 cut(s) 888, 3266
MroXI GAANNNNTTC 2 cut(s) 1519, 2867
MscI TGGCCA 1 cut(s) 2699
MslI CAYNNNNRTG 3 cut(s) 881, 1474, 3453
Msp20I TGGCCA 1 cut(s) 2699
MspA1I CMGCKG 1 cut(s) 3316
MspCI CTTAAG 1 cut(s) 2363
MspI CCGG 4 cut(s) 1199, 2625, 3533, 3554
MspR9I CCNGG 1 cut(s) 3554
MunI CAATTG 1 cut(s) 2555
Mva1269I GAATGC 3 cut(s) 1699, 2393, 3466
MwoI GCNNNNNNNGC 4 cut(s) 103, 1025, 2545, 3355
NciI CCSGG 1 cut(s) 3554
NcoI CCATGG 1 cut(s) 2569
NlaIV GGNNCC 5 cut(s) 282, 755, 1131, 1254, 2415
NmuCI GTSAC 5 cut(s) 437, 2516, 2638, 3325, 3528
NsiI ATGCAT 2 cut(s) 888, 3266
NspI RCATGY 5 cut(s) 845, 1479, 1803, 2787, 3353
NspV TTCGAA 1 cut(s) 1573
PaeI GCATGC 1 cut(s) 3353
PagI TCATGA 1 cut(s) 1855
PceI AGGCCT 1 cut(s) 1152
PciI ACATGT 3 cut(s) 841, 1799, 2783
PciSI GCTCTTC 1 cut(s) 1377
PctI GAATGC 3 cut(s) 1699, 2393, 3466
PdmI GAANNNNTTC 2 cut(s) 1519, 2867
PfeI GAWTC 8 cut(s) 1042, 1774, 1973, 2822, 2893, 2918, 3231, 3566
PflMI CCANNNNNTGG 4 cut(s) 127, 715, 1262, 2695
PkrI GCNGC 6 cut(s) 901, 1018, 1346, 1349, 2039, 3043
PleI GAGTC 6 cut(s) 616, 1099, 2077, 3484, 3635, 3641
PpsI GAGTC 6 cut(s) 616, 1099, 2077, 3484, 3635, 3641
PpuMI RGGWCCY 2 cut(s) 100, 280
PscI ACATGT 3 cut(s) 841, 1799, 2783
PsiI TTATAA 1 cut(s) 630
Psp124BI GAGCTC 1 cut(s) 639
Psp5II RGGWCCY 2 cut(s) 100, 280
PspEI GGTNACC 1 cut(s) 3325
PspFI CCCAGC 1 cut(s) 2152
PspN4I GGNNCC 5 cut(s) 282, 755, 1131, 1254, 2415
PspPI GGNCC 7 cut(s) 45, 100, 280, 706, 1252, 2195, 2847
PspPPI RGGWCCY 2 cut(s) 100, 280
PstI CTGCAG 2 cut(s) 501, 1825
PstNI CAGNNNCTG 2 cut(s) 103, 127
PsuI RGATCY 5 cut(s) 753, 1741, 2156, 2413, 3487
PvuII CAGCTG 1 cut(s) 3316
RsaI GTAC 6 cut(s) 119, 1131, 1686, 1819, 1840, 2979
RsaNI GTAC 6 cut(s) 118, 1130, 1685, 1818, 1839, 2978
RseI CAYNNNNRTG 3 cut(s) 881, 1474, 3453
SacI GAGCTC 1 cut(s) 639
SapI GCTCTTC 1 cut(s) 1377
SatI GCNGC 6 cut(s) 900, 1017, 1345, 1348, 2038, 3042
Sau96I GGNCC 7 cut(s) 45, 100, 280, 706, 1252, 2195, 2847
ScaI AGTACT 3 cut(s) 1819, 1840, 2979
SchI GAGTC 6 cut(s) 617, 1100, 2078, 3485, 3636, 3641
ScrFI CCNGG 1 cut(s) 3554
SduI GDGCHC 3 cut(s) 409, 639, 3575
SfaNI GCATC 7 cut(s) 675, 1309, 1730, 2139, 2359, 3251, 3273
SfcI CTRYAG 3 cut(s) 497, 1540, 1821
SfuI TTCGAA 1 cut(s) 1573
SinI GGWCC 3 cut(s) 100, 280, 1252
SmiMI CAYNNNNRTG 3 cut(s) 881, 1474, 3453
SmlI CTYRAG 3 cut(s) 950, 2278, 2363
SmoI CTYRAG 3 cut(s) 950, 2278, 2363
SphI GCATGC 1 cut(s) 3353
SseBI AGGCCT 1 cut(s) 1152
SsiI CCGC 6 cut(s) 43, 269, 536, 1170, 1656, 2539
SspI AATATT 2 cut(s) 1585, 2383
SstI GAGCTC 1 cut(s) 639
StuI AGGCCT 1 cut(s) 1152
StyD4I CCNGG 1 cut(s) 3552
StyI CCWWGG 1 cut(s) 2569
TaaI ACNGT 8 cut(s) 247, 349, 461, 864, 1544, 1953, 2912, 2982
TaqI TCGA 6 cut(s) 8, 72, 140, 371, 516, 1573
TatI WGTACW 3 cut(s) 1817, 1838, 2977
TfiI GAWTC 8 cut(s) 1042, 1774, 1973, 2822, 2893, 2918, 3231, 3566
TscAI CASTG 7 cut(s) 352, 618, 746, 1956, 2917, 2973, 3370
TseFI GTSAC 5 cut(s) 437, 2516, 2638, 3325, 3528
TseI GCWGC 6 cut(s) 899, 1016, 1344, 1347, 2037, 3041
Tsp45I GTSAC 5 cut(s) 437, 2516, 2638, 3325, 3528
TspGWI ACGGA 2 cut(s) 90, 1576
TspRI CASTG 7 cut(s) 352, 618, 746, 1956, 2917, 2973, 3370
Van91I CCANNNNNTGG 4 cut(s) 127, 715, 1262, 2695
Vha464I CTTAAG 1 cut(s) 2363
VpaK11BI GGWCC 3 cut(s) 100, 280, 1252
XapI RAATTY 5 cut(s) 746, 1462, 2045, 3023, 3337
XbaI TCTAGA 1 cut(s) 2402
XceI RCATGY 5 cut(s) 845, 1479, 1803, 2787, 3353
XmiI GTMKAC 3 cut(s) 60, 2015, 2806
XmnI GAANNNNTTC 2 cut(s) 1519, 2867
ZrmI AGTACT 3 cut(s) 1819, 1840, 2979
Zsp2I ATGCAT 2 cut(s) 888, 3266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.