Rorug04G0183400

FANCI solenoid 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009455
Physical Location & Seq
Forward (+)
32031487 .. 32040066
8580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0183400.1

Sequence Viewer

Length: 4659 bp
ATGGCTGAATTTCTCACTTTTGGGGCTCAAGAATTGCTGAGGAAAGTGGCTTCACTTGCCGCTCAAGAGTTCGATCTTGTATGGGGATTCAATGGAGAAATAACAAAGCTGCGTGAGTCGGTGCTCATGCTTGAGGCTGTGCTACAAGATGCCGAACATCCGCGACAAGATCGGGGAGATGCTGTGAAGCTCTGGGTGAAGAAGCTTGAAGACATAGCTCAGCATGCCGATGATGTGCTGGATGATTATGGATATGAGCTTCTCCGGTGTAAGGTGCAACTGCGAAATCAGATGAAGAGAAAGGTGCAGAACTTTTTTTCCCACTCCAATCCCATCTTTTTTCGTGTTAAAATGGCACATAAAATTAAGAAGATCAACACATCCTTGGATGAGTTGAATAAGAAGGCAAGTGCTATTGGCTTATTTGCTAGACTATCCTTAGATGCAACAACTTCCCATGGTATAAGCATTGACAGGGAAACCTACTCTATCTTAAAACAAGATGAAAACAACATCATTGGAAGGGACGATGTTGTGGCAGATATAGTTCAAGCCTTGACCAAATCAAACCACAATCTGGAAAGTGATCTCTCCGTTCTGGCCATTGTGGGTATGGGAGGGCTTGGAAAGACAACTTTGGCTAAATCAATATATCATGAATCTGAGATAAGTAGGCATTTTGAGAAAAAGATGTGGATATGTGTATCTACCCTTTTTGAAGTCAATTCAATTCTGAGAGGGATCTTGGAATATCTTAAACCAGAAAATGCTGCGGTGAAAGGTATAGATGCAATATGTAACATTCTTCAAGAAGAGTTGAAAGGAAAGAGATACCTTCTTATACTTGATGATGTATGGAATGAAGATGCTGAAAAATGGAATGATTTGAAGAGTTGTTTGGTAAGAATTACTGATGCCCAAGGAAGCAGCATTATTGTCACCACTCGCAGTGACAAAGTTGCAAAAACAGTGGAAACCCTTCCTAGGTGTGATTTGAGAAAACTATCAGATGATGAGTGTTGGCTTATATTGAAGAATAAAGCAGTTCCAGTCGGAAGTGTTCCTATACTTGAAGATCAAGAGACAATTGGAAGGGAGATTGCCAAAAAGTGTGGAGGTATACCATTAGTGGCAAAGGTATTAGGAAACATTATGCGCTTTAAAACAAGCAATGAATGGAGTTCAATTATGGAAAGTAAAATGTGGGATTTACCGAAAGAAGAAGAAAGAATCATGTCAATTTTAAAGTTGAGTTTTGATGAATTGAAATTTTCATCATTGAAACAATGTTTTGCATATTGCTCGGAATTCATCAAAGACTCTGTAATGGAAAAGGATGACTTAGTCCAACTTTGGATGGCTCAAGGATGGCTTCACCCCACCAAAAGTAATATGGAGATGGAGGATAGAGGGAACGAATATTATAACATTTTATCAGAAAAATCCCTTTTTGAAGACGTTAGAACTGATTATAAGGGTAATACAACATGCAAGATGCACGATCTTGTGCATGACCTTGCAGAACGAGTATCAAACATGACAAACTGCCGCTCACTATTTTCCAAGGGAGAAACACTTGGGAACACCTTGCCTAACTTTAGATCTTTACGTGTGTTAAATTTATACAAGGCAGACATTGACAAGTTGCCGAGTTCAATTGGAAAGTTGACACACTTGAGGTATCTGAATGTTATGAAAACAAGAGTGAAGGCATTTCCAAAATCTATTGGTCAACTTTATTACCTTCAGACGTTTAAAATGCCCTATCATGTTGAAGAGTTCCCAAAGATGATAGCTAATATGATCAACTTGAGACATGTTTATTTTGGTAAACATGCGAAAGTTCCTGTTGGCATATTGGGAAGATTGACGAATCTCAGATCATTACCTTTTTTGAAGGTGGGTAAGGAAACTGGGCCTAGGATTGGGGAAATGGGTGGGTTAAACCATTTGAGAGGCACCTTATCCATCTATAATCTGGAGCATGTAAGAGATGGAGAAGAAGCAGTGAATGCAAAGTTAGTTGATAAGAAACATATACGCAAGTTAACTCTCGACTGGAAGCTTAGTAGACCAAGCAACAATATTGACAATGAGGATGATGTACTTGAAGGTCTGCGACCACATTGTAATTTGGAAATTTTGAAGATTCAGGGGTTTATGGGTGTAAAGTTTCCATCATGGTTATTGCTTGCCAGCAACTTGAAAGAAATTGAATTAGCAGGGTGCAACAAATGCGAAGGATTTCCAATACTGGGCCATCTGCCCAATCTTATACATGTTAAGATGGAGGACATGCAGAACCTAAGATGCTTGGGGTCTGAGTTTTATGGTTACGATCAGATTTCAGGTGCAACAACAAGTGACGGGAGAAAGACTTTGTTTCCTGCTTTGAGATCATTACATATTGAGAATGCCGAGAATCTAATAGATTGGATGGAAGCAGGGGAAGTGATGCTGACGACTGGAAAATTAAGGGTGTTTCCTTACCTTGAGGAGCTGCACGTGCTGCGGTGTAAGCAATTGAGAAGTGCTCCCAGTCATTTTCCATCTCTCAAGATGTTGGTGATACGAGGAGTGGAAAGTGGTATGCCTATAGCAAATATTTTAAGCAATAAACTGACTACTCTCACTTATCTCTCAGTATATGATGTCGAGGGACTTACTAGTCTTCCGGAAGGGACGTTAAGAAACAACAAGAGTCTTGAATGTTTGCGTATATGCAACTGTCCGGAGTTAAGCTGCATTTCTCCCCATGGATTTGACTGCTGCGCATCTCTTCAGTTACTGAGAATTTGGAAGTGTCCTAAGTTGAGGTCTTTATCTGATGGGCTACTCCCACTCTCTCTCAAGGAGTTGGACATACAAGATTGCTCCAGTCTAGAGTCCATCCCAATCATCCCTGAGCAAGGCGGTCTCCCATCTCTGCGCAAATTGATTATAACCAAGTGTTCTCAGTTATCCAGTTTACCTGATGGGCTACAATACTGCACATCTCTTCAACAATTGGTAATAGACAGCTGCCCAAAGATAACGTCCATTCCAGTCCAATACTGCACATCTCTTCAACTACTGGAGATACGGAGCTGCCCAAAAATAACGTCCATTCCAACTCAATACTGCACATCTCTTCAACAACTGGACATACTGAGCTGCCCAAAGATAATGTCCATTCCAATTCCATCTGAGGGCCTCCCATATCTTCGTGGACTGCAGCTTTGGCGGTGTCCTGAATTGTCAAGCCTACCGAGTGGACTAGGCTGCTGCCCCTCTCTTGTTGATTTGTCAATAACAGAGTGCCCTAAGGTTACATCCATTCCAATTGACAGTCTCAGTACAACCCTCCGAAGCTTGTGTGTAGATAATCCAGAGTCTCTTCCAATTTTACACGGCGGCTTCACATCCCTCCGAGGCTTGTTAATCGACGGATGTCAAAGTACACAAATTGATCTGGAATTTTGTGCCTTTATTCCAATCCTCGTCTCTCTTGAGGAGTTGAGAATAAGACGGTGTCCTAATCTAGAGACTGTTTCGAGTTTTGACAAGCTCACATCCCTGCGTGAGTTGTGGATTAGTGATTGTTCCCGATTGACATGTCTACCCAGTGGGTTAGCAATGGCATCCCCACACGTATTCACCCGTCTCAAGCAATTGACACTTGGTCGGTTTTGGAATGAGCTGGATTCATTCCCGGCTCTTCAGGCTCTACCACAACTTGAAACACTGACCATCTTCGGGTGGCCGAAGCTCAAGTCTCTGCCTGAACAAGTTCAACACATGACGTCTTTGACCGAATTGCGGATAGATTCCTTTGAGGGAGTGGAGGCTATTCCAGAATGGTTGGGAAACCTTGCATCTCTTCACTCCTTATATATTTTCCGTTGCAAGAATCTGATGTATCTACCTTCCGTCCAAGCTATGCAACGCCTCACCAAATTAGATTGGCTACGTATCTACAATTGTCCCCTTTTGAGCCAAAGATGCACCGCGGAGAGCGGCCCAGAGTGGCCTAAGATTTCTCATCTTCCATCCATCTCTGGACTTGATTCTATCTTGGTGCCAAGCCAACAGGTTCCCTTTCAGAAAATGAAGCTTTCAGTTTGTGCGTTTGAAGTGGCCTTCAAAGTGCTGCATGAAAAGATTAAGACCTTATATGTGGGCAATGACTTCATTGGACATGTTCCTTTAACAAAGAAAATTCTTGCTGCATTAACTACAGGTGCTCTAGCAATCACAGTGGCAAATCCAACTGATCTTGTAAAAGTTAGACTTCAAGCTGAGGGAAAACTACCACCAGGCGCACCAGGGCACTATTCTGGCGCACTAAATGCGTATTCCACCATTTTGAGACAGGAGGGAGTTGGGGATCTCTGGACTGGAATTGGACCTAACATTGCCCGTAATGCTATCATTAATGCTGCTGAACTAGCCAGTTATGATCAAGTGAAGCAGGTTTCTTTGCCATCTGCATTGGCTCTCCAGTTGATGTGCAGAAAACATCCCTCATATTTGTTAATGTTGACGAAGGTTGCACTTAGGACCTTTGGCCTTTATAAAGGCTTCATCCCAAATTTTGGACGGCTAGGATCTTGGAATGTGATCATGTTTCTAACCCTGGAGCAGGCTAAGAAGTTCGTGAAAAGTATAGAGTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
FANCI_HD1 FANCI_HD2 FANCI_S1 FANCI_S2 FANCI_S4

Protein Analysis

1552

Amino Acids

174.38

Weight (kDa)

8.24

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 99 1.5e-20 Rx N-terminal domain
NB-ARC PF00931 179 - 350 5.5e-36 NB-ARC domain
WHD_DRP PF23559 437 - 507 1.1e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 520 - 827 1.6e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 642 - 766 6.1e-36 R13L1/DRL21 LRRs
LRR_14 PF23598 1233 - 1320 1.7e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1255 - 1314 6.6e-07 R13L1/DRL21 LRRs
Mito_carr PF00153 1394 - 1485 9.6e-20 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1425, 1473, 2942, 4554
AasI GACNNNNNNGTC 1 cut(s) 2667
AatII GACGTC 1 cut(s) 3785
Acc16I TGCGCA 2 cut(s) 2773, 2930
Acc36I ACCTGC 1 cut(s) 4441
AccB1I GGYRCC 2 cut(s) 1958, 4057
AccB7I CCANNNNNTGG 2 cut(s) 577, 4574
AccBSI CCGCTC 3 cut(s) 62, 1551, 3996
AccI GTMKAC 3 cut(s) 1120, 2071, 3598
AccII CGCG 2 cut(s) 163, 3989
AccIII TCCGGA 2 cut(s) 2674, 2731
AclWI GGATC 3 cut(s) 749, 4374, 4594
AcoI YGGCCR 2 cut(s) 600, 3740
AcsI RAATTY 9 cut(s) 8, 1268, 1307, 1618, 2139, 2793, 3455, 4197, 4570
AcuI CTGAAG 3 cut(s) 1730, 2765, 3683
AcvI CACGTG 1 cut(s) 2506
AcyI GRCGYC 1 cut(s) 3782
AfaI GTAC 3 cut(s) 2106, 3337, 3439
AflIII ACRYGT 6 cut(s) 1609, 1816, 2278, 3593, 3628, 4177
AhdI GACNNNNNGTC 1 cut(s) 3660
AhlI ACTAGT 1 cut(s) 2666
AjnI CCWGG 3 cut(s) 4294, 4303, 4614
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
Alw21I GWGCWC 3 cut(s) 126, 2536, 4225
AlwI GGATC 3 cut(s) 749, 4374, 4594
AlwNI CAGNNNCTG 1 cut(s) 2788
Aor13HI TCCGGA 2 cut(s) 2674, 2731
AoxI GGCC 9 cut(s) 600, 1916, 2257, 3190, 3740, 3997, 4007, 4116, 4546
ApoI RAATTY 9 cut(s) 8, 1268, 1307, 1618, 2139, 2793, 3455, 4197, 4570
AseI ATTAAT 1 cut(s) 4413
Asp700I GAANNNNTTC 3 cut(s) 978, 2244, 3768
AspA2I CCTAGG 2 cut(s) 983, 1919
AspLEI GCGC 5 cut(s) 1158, 2774, 2931, 4301, 4322
AspS9I GGNCC 6 cut(s) 1916, 2257, 3190, 3998, 4385, 4539
AsuC2I CCSGG 1 cut(s) 3692
AsuHPI GGTGA 7 cut(s) 208, 787, 931, 1367, 2578, 3628, 3922
AvaII GGWCC 2 cut(s) 4385, 4539
AvrII CCTAGG 2 cut(s) 983, 1919
AxyI CCTNAGG 1 cut(s) 3303
BaeGI GKGCMC 2 cut(s) 3302, 4311
BalI TGGCCA 1 cut(s) 602
BanI GGYRCC 2 cut(s) 1958, 4057
BanII GRGCYC 1 cut(s) 28
BbrPI CACGTG 1 cut(s) 2506
BbsI GAAGAC 3 cut(s) 216, 1461, 2663
Bbv12I GWGCWC 3 cut(s) 126, 2536, 4225
BbvCI CCTCAGC 2 cut(s) 38, 4278
BceAI ACGGC 2 cut(s) 3406, 4595
BcgI CGANNNNNNTGC 2 cut(s) 1284, 1318
BciT130I CCWGG 3 cut(s) 4296, 4305, 4616
BclI TGATCA 3 cut(s) 1803, 4438, 4599
BcnI CCSGG 1 cut(s) 3692
BcuI ACTAGT 1 cut(s) 2666
BfmI CTRYAG 3 cut(s) 2595, 3212, 4215
BfuAI ACCTGC 1 cut(s) 4441
BglII AGATCT 1 cut(s) 1601
BlnI CCTAGG 2 cut(s) 983, 1919
BlpI GCTNAGC 1 cut(s) 219
Bme1390I CCNGG 4 cut(s) 3692, 4296, 4305, 4616
Bme18I GGWCC 2 cut(s) 4385, 4539
BmeRI GACNNNNNGTC 1 cut(s) 3660
BmgT120I GGNCC 6 cut(s) 1916, 2257, 3190, 3998, 4385, 4539
BmiI GGNNCC 3 cut(s) 1960, 4059, 4074
BmrFI CCNGG 4 cut(s) 3692, 4296, 4305, 4616
BmrI ACTGGG 4 cut(s) 1923, 2264, 2532, 3597
BmuI ACTGGG 4 cut(s) 1923, 2264, 2532, 3597
BoxI GACNNNNGTC 1 cut(s) 3429
BpiI GAAGAC 3 cut(s) 216, 1461, 2663
BplI GAGNNNNNCTC 2 cut(s) 2518, 2550
BpmI CTGGAG 5 cut(s) 2000, 2860, 3095, 4463, 4637
Bpu10I CCTNAGC 3 cut(s) 38, 2904, 4278
Bpu1102I GCTNAGC 1 cut(s) 219
BpuMI CCSGG 1 cut(s) 3692
BsaAI YACGTR 4 cut(s) 1610, 2506, 3631, 3950
BsaHI GRCGYC 1 cut(s) 3782
BsaI GGTCTC 1 cut(s) 2921
BsaWI WCCGGW 3 cut(s) 264, 2674, 2731
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bse21I CCTNAGG 1 cut(s) 3303
Bse3DI GCAATG 4 cut(s) 1177, 3621, 4168, 4392
BseAI TCCGGA 2 cut(s) 2674, 2731
BseBI CCWGG 3 cut(s) 4296, 4305, 4616
BseMI GCAATG 4 cut(s) 1177, 3621, 4168, 4392
BseRI GAGGAG 3 cut(s) 2510, 2589, 3506
BseSI GKGCMC 2 cut(s) 3302, 4311
BsgI GTGCAG 6 cut(s) 326, 2486, 2974, 3040, 3106, 4510
Bsh1236I CGCG 2 cut(s) 163, 3989
BshFI GGCC 9 cut(s) 602, 1918, 2259, 3192, 3742, 3999, 4009, 4118, 4548
BshNI GGYRCC 2 cut(s) 1958, 4057
BsiHKAI GWGCWC 3 cut(s) 126, 2536, 4225
BsiSI CCGG 4 cut(s) 265, 2675, 2732, 3692
BslFI GGGAC 4 cut(s) 539, 2673, 2695, 3948
BsmBI CGTCTC 2 cut(s) 3487, 3647
BsmFI GGGAC 4 cut(s) 539, 2673, 2695, 3948
BsmI GAATGC 2 cut(s) 2017, 2419
BsnI GGCC 9 cut(s) 602, 1918, 2259, 3192, 3742, 3999, 4009, 4118, 4548
Bso31I GGTCTC 1 cut(s) 2921
Bsp1286I GDGCHC 6 cut(s) 28, 126, 2536, 3302, 4225, 4311
Bsp13I TCCGGA 2 cut(s) 2674, 2731
Bsp1720I GCTNAGC 1 cut(s) 219
Bsp19I CCATGG 2 cut(s) 457, 2755
BspANI GGCC 9 cut(s) 602, 1918, 2259, 3192, 3742, 3999, 4009, 4118, 4548
BspEI TCCGGA 2 cut(s) 2674, 2731
BspFNI CGCG 2 cut(s) 163, 3989
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 3 cut(s) 1960, 4059, 4074
BspMAI CTGCAG 1 cut(s) 3216
BspMI ACCTGC 1 cut(s) 4441
BspPI GGATC 3 cut(s) 749, 4374, 4594
BspQI GCTCTTC 1 cut(s) 3702
BspT107I GGYRCC 2 cut(s) 1958, 4057
BspTNI GGTCTC 1 cut(s) 2921
BsrBI CCGCTC 3 cut(s) 62, 1551, 3996
BsrDI GCAATG 4 cut(s) 1177, 3621, 4168, 4392
BssNAI GTATAC 1 cut(s) 1121
BssNI GRCGYC 1 cut(s) 3782
BssT1I CCWWGG 7 cut(s) 384, 457, 919, 983, 1563, 1919, 2755
Bst1107I GTATAC 1 cut(s) 1121
Bst2UI CCWGG 3 cut(s) 4296, 4305, 4616
Bst4CI ACNGT 6 cut(s) 970, 2729, 3329, 3510, 3529, 4237
BstACI GRCGYC 1 cut(s) 3782
BstAPI GCANNNNNTGC 4 cut(s) 2012, 2235, 2509, 4328
BstBAI YACGTR 4 cut(s) 1610, 2506, 3631, 3950
BstC8I GCNNGC 4 cut(s) 225, 2193, 2197, 4623
BstDSI CCRYGG 3 cut(s) 457, 2755, 3987
BstENI CCTNNNNNAGG 2 cut(s) 2907, 4619
BstFNI CGCG 2 cut(s) 163, 3989
BstHHI GCGC 5 cut(s) 1158, 2774, 2931, 4301, 4322
BstNI CCWGG 3 cut(s) 4296, 4305, 4616
BstNSI RCATGY 9 cut(s) 227, 1491, 1820, 1838, 1988, 2282, 2299, 3597, 4181
BstPAI GACNNNNGTC 1 cut(s) 3429
BstSCI CCNGG 4 cut(s) 3690, 4294, 4303, 4614
BstSFI CTRYAG 3 cut(s) 2595, 3212, 4215
BstSLI GKGCMC 2 cut(s) 3302, 4311
BstSNI TACGTA 1 cut(s) 3950
BstUI CGCG 2 cut(s) 163, 3989
BstV2I GAAGAC 3 cut(s) 216, 1461, 2663
BstX2I RGATCY 4 cut(s) 741, 1601, 4366, 4586
BstYI RGATCY 4 cut(s) 741, 1601, 4366, 4586
BstZ17I GTATAC 1 cut(s) 1121
Bsu36I CCTNAGG 1 cut(s) 3303
BsuRI GGCC 9 cut(s) 602, 1918, 2259, 3192, 3742, 3999, 4009, 4118, 4548
BtgI CCRYGG 3 cut(s) 457, 2755, 3987
BtsI GCAGTG 2 cut(s) 955, 2013
BtsIMutI CAGTG 6 cut(s) 955, 975, 2013, 3610, 3722, 4242
BveI ACCTGC 1 cut(s) 4441
Cac8I GCNNGC 4 cut(s) 225, 2193, 2197, 4623
CaiI CAGNNNCTG 1 cut(s) 2788
CciI TCATGA 1 cut(s) 655
CfoI GCGC 5 cut(s) 1158, 2774, 2931, 4301, 4322
Cfr13I GGNCC 6 cut(s) 1916, 2257, 3190, 3998, 4385, 4539
Cfr42I CCGCGG 1 cut(s) 3990
Csp6I GTAC 3 cut(s) 2105, 3336, 3438
CspCI CAANNNNNGTGG 6 cut(s) 951, 986, 1093, 1128, 4218, 4253
CviQI GTAC 3 cut(s) 2105, 3336, 3438
DraI TTTAAA 3 cut(s) 1162, 1245, 1756
DrdI GACNNNNNNGTC 1 cut(s) 2667
DriI GACNNNNNGTC 1 cut(s) 3660
DseDI GACNNNNNNGTC 1 cut(s) 2667
EaeI YGGCCR 2 cut(s) 600, 3740
Eam1105I GACNNNNNGTC 1 cut(s) 3660
Eco105I TACGTA 1 cut(s) 3950
Eco130I CCWWGG 7 cut(s) 384, 457, 919, 983, 1563, 1919, 2755
Eco24I GRGCYC 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 2921
Eco47I GGWCC 2 cut(s) 4385, 4539
Eco57I CTGAAG 3 cut(s) 1730, 2765, 3683
Eco72I CACGTG 1 cut(s) 2506
Eco81I CCTNAGG 1 cut(s) 3303
EcoNI CCTNNNNNAGG 2 cut(s) 2907, 4619
EcoO109I RGGNCCY 2 cut(s) 3190, 4539
EcoRI GAATTC 1 cut(s) 1307
EcoRII CCWGG 3 cut(s) 4294, 4303, 4614
EcoT14I CCWWGG 7 cut(s) 384, 457, 919, 983, 1563, 1919, 2755
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 7 cut(s) 384, 457, 919, 983, 1563, 1919, 2755
Esp3I CGTCTC 2 cut(s) 3487, 3647
FalI AAGNNNNNCTT 6 cut(s) 1325, 1357, 1356, 1388, 4254, 4286
FaqI GGGAC 4 cut(s) 539, 2673, 2695, 3948
FbaI TGATCA 3 cut(s) 1803, 4438, 4599
FblI GTMKAC 3 cut(s) 1120, 2071, 3598
FriOI GRGCYC 1 cut(s) 28
FspI TGCGCA 2 cut(s) 2773, 2930
GlaI GCGC 5 cut(s) 1157, 2773, 2930, 4300, 4321
GsuI CTGGAG 5 cut(s) 2000, 2860, 3095, 4463, 4637
HaeIII GGCC 9 cut(s) 602, 1918, 2259, 3192, 3742, 3999, 4009, 4118, 4548
HapII CCGG 4 cut(s) 265, 2675, 2732, 3692
HhaI GCGC 5 cut(s) 1158, 2774, 2931, 4301, 4322
Hin1I GRCGYC 1 cut(s) 3782
Hin6I GCGC 5 cut(s) 1156, 2772, 2929, 4299, 4320
HinP1I GCGC 5 cut(s) 1156, 2772, 2929, 4299, 4320
HincII GTYRAC 4 cut(s) 1668, 1733, 2049, 4521
HindII GTYRAC 4 cut(s) 1668, 1733, 2049, 4521
HindIII AAGCTT 4 cut(s) 203, 2063, 3349, 4091
HpaI GTTAAC 1 cut(s) 2049
HpaII CCGG 4 cut(s) 265, 2675, 2732, 3692
HphI GGTGA 7 cut(s) 208, 787, 931, 1367, 2578, 3628, 3922
Hpy99I CGWCG 1 cut(s) 3428
HpyCH4III ACNGT 6 cut(s) 970, 2729, 3329, 3510, 3529, 4237
Hsp92I GRCGYC 1 cut(s) 3782
HspAI GCGC 5 cut(s) 1156, 2772, 2929, 4299, 4320
Kpn2I TCCGGA 2 cut(s) 2674, 2731
Ksp22I TGATCA 3 cut(s) 1803, 4438, 4599
KspAI GTTAAC 1 cut(s) 2049
KspI CCGCGG 1 cut(s) 3990
LguI GCTCTTC 1 cut(s) 3702
LmnI GCTCC 6 cut(s) 1981, 2497, 2539, 2879, 3084, 4618
MaeIII GTNAC 7 cut(s) 797, 937, 950, 2333, 2363, 2784, 3307
MbiI CCGCTC 3 cut(s) 62, 1551, 3996
MfeI CAATTG 7 cut(s) 1086, 1656, 2522, 3005, 3321, 3650, 3958
MflI RGATCY 4 cut(s) 741, 1601, 4366, 4586
MhlI GDGCHC 6 cut(s) 28, 126, 2536, 3302, 4225, 4311
MlsI TGGCCA 1 cut(s) 602
MluNI TGGCCA 1 cut(s) 602
MlyI GAGTC 6 cut(s) 125, 1313, 2710, 2894, 3380, 4658
MmeI TCCRAC 5 cut(s) 1033, 1372, 2838, 3134, 4271
Mox20I TGGCCA 1 cut(s) 602
MroI TCCGGA 2 cut(s) 2674, 2731
MroXI GAANNNNTTC 3 cut(s) 978, 2244, 3768
MscI TGGCCA 1 cut(s) 602
MslI CAYNNNNRTG 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 602
MspA1I CMGCKG 2 cut(s) 3021, 3989
MspI CCGG 4 cut(s) 265, 2675, 2732, 3692
MspR9I CCNGG 4 cut(s) 3692, 4296, 4305, 4616
MunI CAATTG 7 cut(s) 1086, 1656, 2522, 3005, 3321, 3650, 3958
Mva1269I GAATGC 2 cut(s) 2017, 2419
MvaI CCWGG 3 cut(s) 4296, 4305, 4616
MvnI CGCG 2 cut(s) 163, 3989
NciI CCSGG 1 cut(s) 3692
NcoI CCATGG 2 cut(s) 457, 2755
NlaIV GGNNCC 3 cut(s) 1960, 4059, 4074
NmeAIII GCCGAG 2 cut(s) 1674, 2443
NmuCI GTSAC 3 cut(s) 937, 950, 2363
NsbI TGCGCA 2 cut(s) 2773, 2930
NspI RCATGY 9 cut(s) 227, 1491, 1820, 1838, 1988, 2282, 2299, 3597, 4181
PaeI GCATGC 1 cut(s) 227
PagI TCATGA 1 cut(s) 655
PciI ACATGT 4 cut(s) 1816, 2278, 3593, 4177
PciSI GCTCTTC 1 cut(s) 3702
PctI GAATGC 2 cut(s) 2017, 2419
PdmI GAANNNNTTC 3 cut(s) 978, 2244, 3768
PflFI GACNNNGTC 2 cut(s) 1343, 3510
PflMI CCANNNNNTGG 2 cut(s) 577, 4574
PleI GAGTC 6 cut(s) 124, 1313, 2709, 2893, 3379, 4657
PmaCI CACGTG 1 cut(s) 2506
PmlI CACGTG 1 cut(s) 2506
PpsI GAGTC 6 cut(s) 124, 1313, 2709, 2893, 3379, 4657
Ppu21I YACGTR 4 cut(s) 1610, 2506, 3631, 3950
PpuMI RGGWCCY 1 cut(s) 4539
PscI ACATGT 4 cut(s) 1816, 2278, 3593, 4177
PshAI GACNNNNGTC 1 cut(s) 3429
PshBI ATTAAT 1 cut(s) 4413
PsiI TTATAA 4 cut(s) 1425, 1473, 2942, 4554
Psp5II RGGWCCY 1 cut(s) 4539
Psp6I CCWGG 3 cut(s) 4294, 4303, 4614
PspCI CACGTG 1 cut(s) 2506
PspGI CCWGG 3 cut(s) 4294, 4303, 4614
PspN4I GGNNCC 3 cut(s) 1960, 4059, 4074
PspPI GGNCC 6 cut(s) 1916, 2257, 3190, 3998, 4385, 4539
PspPPI RGGWCCY 1 cut(s) 4539
PstI CTGCAG 1 cut(s) 3216
PstNI CAGNNNCTG 1 cut(s) 2788
PsuI RGATCY 4 cut(s) 741, 1601, 4366, 4586
PsyI GACNNNGTC 2 cut(s) 1343, 3510
PvuII CAGCTG 1 cut(s) 3021
RsaI GTAC 3 cut(s) 2106, 3337, 3439
RsaNI GTAC 3 cut(s) 2105, 3336, 3438
RseI CAYNNNNRTG 1 cut(s) 228
SacII CCGCGG 1 cut(s) 3990
SapI GCTCTTC 1 cut(s) 3702
Sau96I GGNCC 6 cut(s) 1916, 2257, 3190, 3998, 4385, 4539
SchI GAGTC 6 cut(s) 125, 1313, 2710, 2894, 3380, 4658
ScrFI CCNGG 4 cut(s) 3692, 4296, 4305, 4616
SduI GDGCHC 6 cut(s) 28, 126, 2536, 3302, 4225, 4311
SfcI CTRYAG 3 cut(s) 2595, 3212, 4215
Sfr303I CCGCGG 1 cut(s) 3990
SgrBI CCGCGG 1 cut(s) 3990
SinI GGWCC 2 cut(s) 4385, 4539
SmiMI CAYNNNNRTG 1 cut(s) 228
SnaBI TACGTA 1 cut(s) 3950
SpeI ACTAGT 1 cut(s) 2666
SphI GCATGC 1 cut(s) 227
SspI AATATT 3 cut(s) 1421, 2086, 2605
StyD4I CCNGG 4 cut(s) 3690, 4294, 4303, 4614
StyI CCWWGG 7 cut(s) 384, 457, 919, 983, 1563, 1919, 2755
TaaI ACNGT 6 cut(s) 970, 2729, 3329, 3510, 3529, 4237
TaqI TCGA 5 cut(s) 72, 2055, 2655, 3423, 3533
TaqII GACCGA 1 cut(s) 3806
TatI WGTACW 3 cut(s) 2104, 3335, 3437
TauI GCSGC 4 cut(s) 62, 1551, 3396, 3999
TscAI CASTG 6 cut(s) 955, 975, 2013, 3610, 3729, 4242
TseFI GTSAC 3 cut(s) 937, 950, 2363
Tsp45I GTSAC 3 cut(s) 937, 950, 2363
TspGWI ACGGA 5 cut(s) 583, 3097, 3441, 3869, 3898
TspRI CASTG 6 cut(s) 955, 975, 2013, 3610, 3729, 4242
Tth111I GACNNNGTC 2 cut(s) 1343, 3510
Van91I CCANNNNNTGG 2 cut(s) 577, 4574
VpaK11BI GGWCC 2 cut(s) 4385, 4539
VspI ATTAAT 1 cut(s) 4413
XagI CCTNNNNNAGG 2 cut(s) 2907, 4619
XapI RAATTY 9 cut(s) 8, 1268, 1307, 1618, 2139, 2793, 3455, 4197, 4570
XbaI TCTAGA 2 cut(s) 2881, 3520
XceI RCATGY 9 cut(s) 227, 1491, 1820, 1838, 1988, 2282, 2299, 3597, 4181
XcmI CCANNNNNNNNNTGG 3 cut(s) 610, 1390, 1975
XmaJI CCTAGG 2 cut(s) 983, 1919
XmiI GTMKAC 3 cut(s) 1120, 2071, 3598
XmnI GAANNNNTTC 3 cut(s) 978, 2244, 3768
ZraI GACGTC 1 cut(s) 3783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.