Rroxscaffold_5G00365140

FANCI solenoid 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009455
Physical Location & Seq
Forward (+)
46475551 .. 46482660
7110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365140.1

Sequence Viewer

Length: 1830 bp
ATGTTACTTCTACAACCAAATGGAAAAACTGAGAAAGCCTTGAATTCTTCATGGGCATGTTTGGGTTTCTCCCTTTCACAAGAAAACGAGGTAGGAAGAAATTCGTCTGCTGACTCATTCTACAGTTCTTTCTTGAAGATTCGGAAGTCTCTACGAAATCAGAATATTGAAGGCTTCTTTGGTCAAACTCAGGATGGAGGCTCTTCATCTTTCGAAGACAACAAAAGAAAATGCTCCACTTTTGTTTTATCAGGAATTATTGAAGTACTCGTGAATGCTATTGCTACTGAGTTGGACAAAGCATCGGATAGAACGAAATTAGATCTTGAAAAGGAGCTTATTCAGTTTGTTGAGCTTCATCATTCACTCAATAAGGATACATGTTTCTTCAGCAAGAGTACTGCAGTAAGAAAAGGGAGTACTCGCACTCCTGCTCATGATATACTGAACAACATCAATTCAAGCCACTCTAAAGAAATGCAAGGACGGATTTCCTTCTTTGCAACTTCAAGCATATATCAGATATTGCAAACACTGTTGAGACTACTTGATACTGATTATTCTAATAATGTTGAAGCTTCCCAAATGCATAGTCAGTCTACCAGCAAGACATCCAAATGCAGCTCTGAATTTATTTCTTTTGTCTTGAATGTGTCCCTTAGTCATATAAAATCTTCCCTTCTTCTGAGAAATGAAGACCCTTTGAGAACTTTAATCTATGGGGAAATCAATATCCTGGGGTCCCCCTTGTTGAAACTAATTTCTTTGTTCAAATCAGGGCCAAAGTCGGTGATGGATCAAAAGAAAAAGGAAGCAAAGGGGAAAAAAGATGTTGGACGAAGGGAGCATCTTCATCTGGCCTTGATTTGCTTGAAGGAATTGATAATGATTTGTTCTAATTCAGACTTGACTGGTTTGCTTGAAGACATGGTGTCAATTTCCACTCTTCAGGATGCTGATTTGGAAACTGAATGCGAAAGAGCCTCTAGAATTGAGGATCCACATATAAGGAGCAAAGAACTGTTCATTTTGAAGACATTGAAGCCTCTATTTTCTGAGCTTCTTGCAGTTTCATTATTTGGTGAAGTTGAGATTATTTGTGACCTGATATTGATGATAGGGGGAAAATTGCCATGCCAATTGAGGAACTCCCATGGAGCTTGGGCTATTAGTCTTTGCAAAAACAATGAAATAAAAAACACGAAGGTTGGAAAAAGTGTTGTGACACTTGCTATAAGTTTAAGCTCCCCTCCAAATGATCTAGCTATATCTCAAGACATGGCTAGAGAGCTGTTACAGGTTATTGGATCAGAGGGAGATACTCCAGAGGAGGTGTCCGAGATATACCCCCTCATAAACCATCAAACAAGTTCAGCAATAAATTCCTGTATACTGCACATTGTTGAAGCAGTTGTAGTTGACATGGATTGGGCCATCAAAAGATTGAAGACATTCTCTTTAGTCTCCCAAAAAGGGATTCATCTGAATCAGAACAGTGAGGAATCTCCTGAGTTGGCATTTGAGGAAAATCTCTATGCTAGGGCAGAAGCAGTGGTGAAAGTACTATCATCTTTTGTTTCAATGAGCCTGAATGATACGCAAGCTGAGCATGTGCTGCGACTGGCAGCAAGGTTTTACAAGCATTTAGCTCAGATGTCAAAGCTTAGGATTGCTACAAAAGGTTGTAAACAGATTTTTCCGAGCCTCAAGTTTCAAAAGCTTGTGGAGGTTACTTGCAAGGAGCTGACAGTTCCTCTGTATATCTTTGTGGAAATTATGCAAAAGAAACAACAAGAGAATGCCCATAAAAGGGAATTATCAACAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
FANCI_HD1 FANCI_HD2 FANCI_S1 FANCI_S2 FANCI_S4

Protein Analysis

609

Amino Acids

68.06

Weight (kDa)

7.88

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FANCI_HD2 PF14680 25 - 127 2.7e-12 FANCI helical domain 2
FANCI_S4 PF14678 425 - 604 3.8e-44 FANCI solenoid 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 599, 1390
AclWI GGATC 4 cut(s) 804, 992, 1005, 1315
AcsI RAATTY 4 cut(s) 43, 100, 629, 1381
AcuI CTGAAG 2 cut(s) 373, 932
AfaI GTAC 4 cut(s) 267, 400, 421, 1563
AfiI CCNNNNNNNGG 3 cut(s) 1473, 1809, 1810
AflIII ACRYGT 1 cut(s) 380
AhdI GACNNNNNGTC 1 cut(s) 931
AjnI CCWGG 1 cut(s) 735
AjuI GAANNNNNNNTTGG 4 cut(s) 162, 194, 1461, 1493
Alw26I GTCTC 3 cut(s) 153, 535, 1468
AlwI GGATC 4 cut(s) 804, 992, 1005, 1315
AoxI GGCC 3 cut(s) 779, 858, 1431
ApeKI GCWGC 3 cut(s) 621, 1615, 1625
ApoI RAATTY 4 cut(s) 43, 100, 629, 1381
ArsI GACNNNNNNTTYG 2 cut(s) 577, 609
Asp700I GAANNNNTTC 2 cut(s) 100, 1451
AspS9I GGNCC 3 cut(s) 741, 779, 1431
AsuHPI GGTGA 3 cut(s) 802, 1094, 1567
AsuII TTCGAA 1 cut(s) 213
AvaII GGWCC 1 cut(s) 741
BamHI GGATCC 1 cut(s) 997
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 5 cut(s) 222, 702, 930, 1040, 1454
BbvI GCAGC 3 cut(s) 633, 1602, 1637
BccI CCATC 4 cut(s) 188, 787, 1368, 1442
BciT130I CCWGG 1 cut(s) 737
BciVI GTATCC 1 cut(s) 370
BcoDI GTCTC 3 cut(s) 153, 535, 1468
BfaI CTAG 4 cut(s) 987, 1262, 1284, 1539
BfmI CTRYAG 2 cut(s) 121, 402
BfuI GTATCC 1 cut(s) 370
BglII AGATCT 1 cut(s) 322
BisI GCNGC 3 cut(s) 622, 1616, 1626
BlpI GCTNAGC 1 cut(s) 1605
BlsI GCNGC 3 cut(s) 623, 1617, 1627
BmcAI AGTACT 4 cut(s) 267, 400, 421, 1563
Bme1390I CCNGG 1 cut(s) 737
Bme18I GGWCC 1 cut(s) 741
BmeRI GACNNNNNGTC 1 cut(s) 931
BmgT120I GGNCC 3 cut(s) 741, 779, 1431
BmiI GGNNCC 3 cut(s) 742, 743, 999
BmrFI CCNGG 1 cut(s) 737
BmsI GCATC 3 cut(s) 311, 856, 943
BpiI GAAGAC 5 cut(s) 222, 702, 930, 1040, 1454
BpmI CTGGAG 1 cut(s) 1308
Bpu10I CCTNAGC 1 cut(s) 1664
Bpu1102I GCTNAGC 1 cut(s) 1605
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 2 cut(s) 1257, 1691
BsaJI CCNNGG 2 cut(s) 736, 1153
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 3 cut(s) 1473, 1809, 1810
Bse1I ACTGG 2 cut(s) 916, 1626
BseBI CCWGG 1 cut(s) 737
BseDI CCNNGG 2 cut(s) 736, 1153
BseGI GGATG 3 cut(s) 199, 611, 958
BseLI CCNNNNNNNGG 3 cut(s) 1473, 1809, 1810
BseMII CTCAG 8 cut(s) 21, 203, 279, 677, 1047, 1500, 1596, 1664
BseNI ACTGG 2 cut(s) 916, 1626
BseRI GAGGAG 1 cut(s) 1343
BseXI GCAGC 3 cut(s) 633, 1602, 1637
BsgI GTGCAG 1 cut(s) 1379
BshFI GGCC 3 cut(s) 781, 860, 1433
BslFI GGGAC 2 cut(s) 640, 727
BslI CCNNNNNNNGG 3 cut(s) 1473, 1809, 1810
BsmAI GTCTC 3 cut(s) 153, 535, 1468
BsmFI GGGAC 2 cut(s) 640, 727
BsmI GAATGC 3 cut(s) 280, 977, 1804
BsnI GGCC 3 cut(s) 781, 860, 1433
Bsp119I TTCGAA 1 cut(s) 213
Bsp143I GATC 5 cut(s) 322, 796, 997, 1258, 1307
Bsp1720I GCTNAGC 1 cut(s) 1605
Bsp19I CCATGG 1 cut(s) 1153
BspANI GGCC 3 cut(s) 781, 860, 1433
BspCNI CTCAG 8 cut(s) 22, 202, 280, 678, 1048, 1501, 1597, 1663
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 3 cut(s) 742, 743, 999
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 4 cut(s) 804, 992, 1005, 1315
BspQI GCTCTTC 1 cut(s) 208
BspT104I TTCGAA 1 cut(s) 213
BsrI ACTGG 2 cut(s) 916, 1626
BssECI CCNNGG 2 cut(s) 736, 1153
BssMI GATC 5 cut(s) 322, 796, 997, 1258, 1307
BssNAI GTATAC 1 cut(s) 1391
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 1153
Bst1107I GTATAC 1 cut(s) 1391
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 5 cut(s) 125, 537, 1023, 1496, 1750
Bst6I CTCTTC 2 cut(s) 208, 951
BstAPI GCANNNNNTGC 1 cut(s) 1615
BstBI TTCGAA 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 1602
BstDSI CCRYGG 1 cut(s) 1153
BstF5I GGATG 3 cut(s) 199, 611, 958
BstKTI GATC 5 cut(s) 325, 799, 1000, 1261, 1310
BstMAI GTCTC 3 cut(s) 153, 535, 1468
BstMBI GATC 5 cut(s) 322, 796, 997, 1258, 1307
BstMWI GCNNNNNNNGC 2 cut(s) 1606, 1615
BstNI CCWGG 1 cut(s) 737
BstNSI RCATGY 3 cut(s) 60, 384, 1613
BstSCI CCNGG 1 cut(s) 735
BstSFI CTRYAG 2 cut(s) 121, 402
BstV1I GCAGC 3 cut(s) 633, 1602, 1637
BstV2I GAAGAC 5 cut(s) 222, 702, 930, 1040, 1454
BstX2I RGATCY 2 cut(s) 322, 997
BstYI RGATCY 2 cut(s) 322, 997
BstZ17I GTATAC 1 cut(s) 1391
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 3 cut(s) 781, 860, 1433
BtgI CCRYGG 1 cut(s) 1153
BtsCI GGATG 3 cut(s) 199, 611, 958
BtsI GCAGTG 1 cut(s) 1557
BtsIMutI CAGTG 3 cut(s) 533, 1501, 1557
Cac8I GCNNGC 1 cut(s) 1602
CciI TCATGA 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 741, 779, 1431
Csp6I GTAC 4 cut(s) 266, 399, 420, 1562
CviQI GTAC 4 cut(s) 266, 399, 420, 1562
DpnI GATC 5 cut(s) 324, 798, 999, 1260, 1309
DpnII GATC 5 cut(s) 322, 796, 997, 1258, 1307
DriI GACNNNNNGTC 1 cut(s) 931
Eam1104I CTCTTC 2 cut(s) 208, 951
Eam1105I GACNNNNNGTC 1 cut(s) 931
EarI CTCTTC 2 cut(s) 208, 951
Eco130I CCWWGG 1 cut(s) 1153
Eco47I GGWCC 1 cut(s) 741
Eco57I CTGAAG 2 cut(s) 373, 932
EcoO109I RGGNCCY 1 cut(s) 741
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 735
EcoT14I CCWWGG 1 cut(s) 1153
EcoT22I ATGCAT 1 cut(s) 591
ErhI CCWWGG 1 cut(s) 1153
FaqI GGGAC 2 cut(s) 640, 727
FblI GTMKAC 2 cut(s) 599, 1390
Fnu4HI GCNGC 3 cut(s) 622, 1616, 1626
FokI GGATG 3 cut(s) 206, 598, 965
Fsp4HI GCNGC 3 cut(s) 622, 1616, 1626
FspBI CTAG 4 cut(s) 987, 1262, 1284, 1539
GluI GCNGC 3 cut(s) 622, 1616, 1626
GsuI CTGGAG 1 cut(s) 1308
HaeIII GGCC 3 cut(s) 781, 860, 1433
HincII GTYRAC 1 cut(s) 1420
HindII GTYRAC 1 cut(s) 1420
HindIII AAGCTT 3 cut(s) 576, 1661, 1718
HinfI GANTC 5 cut(s) 113, 139, 1477, 1486, 1502
HphI GGTGA 3 cut(s) 802, 1094, 1567
Hpy166II GTNNAC 4 cut(s) 600, 1391, 1420, 1688
Hpy8I GTNNAC 4 cut(s) 600, 1391, 1420, 1688
HpyAV CCTTC 6 cut(s) 164, 505, 689, 834, 868, 1198
HpyCH4III ACNGT 5 cut(s) 125, 537, 1023, 1496, 1750
HpyF10VI GCNNNNNNNGC 2 cut(s) 1606, 1615
KflI GGGWCCC 1 cut(s) 741
Kzo9I GATC 5 cut(s) 322, 796, 997, 1258, 1307
LguI GCTCTTC 1 cut(s) 208
LmnI GCTCC 7 cut(s) 239, 334, 844, 1011, 1157, 1250, 1741
Lsp1109I GCAGC 3 cut(s) 633, 1602, 1637
LweI GCATC 3 cut(s) 311, 856, 943
MaeI CTAG 4 cut(s) 987, 1262, 1284, 1539
MaeIII GTNAC 5 cut(s) 3, 1100, 1222, 1293, 1729
MalI GATC 5 cut(s) 324, 798, 999, 1260, 1309
MboI GATC 5 cut(s) 322, 796, 997, 1258, 1307
MfeI CAATTG 1 cut(s) 1139
MflI RGATCY 2 cut(s) 322, 997
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 3 cut(s) 273, 814, 1189
Mph1103I ATGCAT 1 cut(s) 591
MroXI GAANNNNTTC 2 cut(s) 100, 1451
MseI TTAA 2 cut(s) 713, 1241
MslI CAYNNNNRTG 2 cut(s) 55, 616
MspR9I CCNGG 1 cut(s) 737
MunI CAATTG 1 cut(s) 1139
Mva1269I GAATGC 3 cut(s) 280, 977, 1804
MvaI CCWGG 1 cut(s) 737
MwoI GCNNNNNNNGC 2 cut(s) 1606, 1615
NcoI CCATGG 1 cut(s) 1153
NdeII GATC 5 cut(s) 322, 796, 997, 1258, 1307
NlaIV GGNNCC 3 cut(s) 742, 743, 999
NmuCI GTSAC 2 cut(s) 1100, 1222
NsiI ATGCAT 1 cut(s) 591
NspI RCATGY 3 cut(s) 60, 384, 1613
NspV TTCGAA 1 cut(s) 213
PagI TCATGA 1 cut(s) 436
PciI ACATGT 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PctI GAATGC 3 cut(s) 280, 977, 1804
PdmI GAANNNNTTC 2 cut(s) 100, 1451
PfeI GAWTC 4 cut(s) 139, 1477, 1486, 1502
PkrI GCNGC 3 cut(s) 623, 1617, 1627
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 741
PscI ACATGT 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 741
Psp6I CCWGG 1 cut(s) 735
PspGI CCWGG 1 cut(s) 735
PspN4I GGNNCC 3 cut(s) 742, 743, 999
PspPI GGNCC 3 cut(s) 741, 779, 1431
PspPPI RGGWCCY 1 cut(s) 741
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 2 cut(s) 322, 997
RsaI GTAC 4 cut(s) 267, 400, 421, 1563
RsaNI GTAC 4 cut(s) 266, 399, 420, 1562
RseI CAYNNNNRTG 2 cut(s) 55, 616
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 2 cut(s) 713, 1241
SatI GCNGC 3 cut(s) 622, 1616, 1626
Sau3AI GATC 5 cut(s) 322, 796, 997, 1258, 1307
Sau96I GGNCC 3 cut(s) 741, 779, 1431
ScaI AGTACT 4 cut(s) 267, 400, 421, 1563
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 737
SfaNI GCATC 3 cut(s) 311, 856, 943
SfcI CTRYAG 2 cut(s) 121, 402
SfuI TTCGAA 1 cut(s) 213
SinI GGWCC 1 cut(s) 741
SmiMI CAYNNNNRTG 2 cut(s) 55, 616
SmlI CTYRAG 2 cut(s) 1272, 1706
SmoI CTYRAG 2 cut(s) 1272, 1706
SspI AATATT 1 cut(s) 166
SspMI CTAG 4 cut(s) 987, 1262, 1284, 1539
StyD4I CCNGG 1 cut(s) 735
StyI CCWWGG 1 cut(s) 1153
TaaI ACNGT 5 cut(s) 125, 537, 1023, 1496, 1750
TaqI TCGA 1 cut(s) 213
TatI WGTACW 4 cut(s) 265, 398, 419, 1561
TfiI GAWTC 4 cut(s) 139, 1477, 1486, 1502
Tru1I TTAA 2 cut(s) 713, 1241
Tru9I TTAA 2 cut(s) 713, 1241
TscAI CASTG 3 cut(s) 540, 1501, 1557
TseFI GTSAC 2 cut(s) 1100, 1222
TseI GCWGC 3 cut(s) 621, 1615, 1625
Tsp45I GTSAC 2 cut(s) 1100, 1222
TspDTI ATGAA 9 cut(s) 39, 195, 347, 708, 842, 1015, 1062, 1203, 1469
TspGWI ACGGA 1 cut(s) 502
TspRI CASTG 3 cut(s) 540, 1501, 1557
VpaK11BI GGWCC 1 cut(s) 741
XapI RAATTY 4 cut(s) 43, 100, 629, 1381
XbaI TCTAGA 1 cut(s) 986
XceI RCATGY 3 cut(s) 60, 384, 1613
XmiI GTMKAC 2 cut(s) 599, 1390
XmnI GAANNNNTTC 2 cut(s) 100, 1451
XspI CTAG 4 cut(s) 987, 1262, 1284, 1539
ZrmI AGTACT 4 cut(s) 267, 400, 421, 1563
Zsp2I ATGCAT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.