Rh4CG257700

FANCI solenoid 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009455
Physical Location & Seq
Forward (+)
51049388 .. 51056647
7260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG257700.1

Sequence Viewer

Length: 4218 bp
ATGAGCGCAACCACACCCTCCTCACCATTTCTAACAGACTCCGATATCATCCGCTTAGCCCAGTCCTGCGCCCCAATCCCCTCCTCCTTTCTCTCCACCCAATCCCACCCAACCCTCCTATCCTACCTCACTTCCCGCTCTCAATCCTCCTCACCATCAAAACCAGTCTCCGACTACACACTCTCTCTCCTCTCCCTCATCTCCCAATCTCCCCAAACCCACTCTCTCTCTACCCTCCTCTCCTCTCTCCTCCTCTCCTACACCTACCTCTTCACCTCCTTTCAAATCCCACACGACCCCAACTCCCTCAAGACCCTCCAATTCTTTCACACCCTATTACCCCACGTACCCACTTCTCATCTCCCCCAAATAATCGAACCCATTTTGTCATTTTTCCCCCAAATTACCGACTCCGAAAACGCCCAGATCCTCGATCTCCTCCCCCGGTGCTTGACCTTGATTCGAAGCTCCGACGAGGTCGAACGCGGCGGCCATGTCGTCAATTCGGTGCTCGATCAGATTCTGGAGCTCAATTGGTCCAAAGGACTGTTGATCAAGATGGTTTCGATCGTAAGGGACTTTCCTGTTCTTGATAAATTGAGGGCCAGAGAGTTTGTAGACAAGGTGTTCGATGGAATGTCTAGCTTAGACTTACAAGACTTGCCGTCGCTTGTCTACCAATTGCTGGTTTTGGCGTCGAAAGGGTTTGTTAAGAAGGATGTGATTGAAGGGATTGTGATGTTTTTTGGGTCGAAAATGGGGAGGAAGAAGGCGAGCTCTATAGTCAGGCAAGTGGAAGGTACTGTTTTGCTGCATGTCAACTTTGCGGTGAAGCAGGACCCTTCATTGGGACAAGAGGTGATGGGGCTTGTGAAGTCTGATCTTAGAGCTTTCAATCATTTTACTGTTGCTGTTTTGTTATCGGTTTCGAGGGTGAGGAGGTTTGGTGAGGCATCAATGGGGGCTTTGAAAATTGCGATTCTAACTGCATACCGTGATTACAAGTTTGCTAAAGAATGTAAATGGATACCAGATGACATGAAGGAAGAATATCTGCACAATGTCAAAGTGGTCGAGAAGGCCATGTTAAGAGCGGTTAATGAGAGCAACTATGGAAGGGAACACATTGTGCCTAGCATTTTGCAGTTTGCTTTTGTATTGCTAGAGTCAGCGGAAGAGGGAAACCGTAAAGAGCTCTGCAATTTAAGTGGTTTGGTAGGCATTGAGGAGCTTGCTATTCAGATGCTAAAAGCTATATTTGTGGCCCATGATATGGCTAGAAATGAGATTATTGAGCAGTGTAAATTTCGGATCCTTTCTTTGAAGCCAGAGCAGAGCATGGTTATCATAAGACTTCTCAGTAATCTAGCTCAAAGTTATCCGTACCCCATGTTAGAACATGTTTCCCGGCTAAAGGAATTGTTGGATTACTTCACTTTCATGCATGGCAAGGTTGCTGCTCAACTTGTTACTGCTTTGTTGCCTCTCATCAAGTTCAGCCGTGATCTCAAGGATTACACTATTTTGGTAATGCGTAAGGCGATGTTTAGACGAGAAGATACAATTCGTCTTGCAGCAACCAATGCTGTTATTGATTTGATTCTGGCAGAAAAGCAATCCAAGAAAGATGATTTGATGTCTTTTCAAGAGTCATCTAGCCAAGCCAGTAGCAGTCAGCAATCTGAGATACCCTACAATGTTGGGGGAGGCCTGTTTCAAGAGCTCAGTGGTCTATTGCAGAGATGTCTTTATCAACCGGCAAGTGTCAAAGAAGTCATGTATCATGGACTTGTGAAGCTTGTGTTGATGGACCCATCTAGTGGGGGGGCTGTCTTTGATTTTTTGTTGCCTCACTTCCTCCATTTTTTTAAAGAGGATGCAGATGTTCAATTAGGAATTAGCTGCTGCATAAAATCAGAGAGTGGCAAAGCTTATGTTGAAGAGCCCTTAGATTGCCTTCTGTCTTGTGTTTCTTGGATGTTACTTCTACAACCAAATGGAAAAACTGAGAAAGCCTTGAATTCTTCATGGGCATGTTTGGGTTTCTCCCTTTCACAAGAAAACGAGGTAGGAAGAAATTCGTCTGTTGACTCATTCTACAGTTCTTTCTTGAAGATTCGGAAGTCTCTACGAAATCAGAATATTGAAGGCATCTTTGGTCAAACTCAGGATGGAGGCTCTTCATCTTTTGAAGACAACAAAAGAAAATGCTCCGCTTTTGTTTTATCAGGAATTATTGAAGTACTCGTGAATGCTATTGCTACTGAGTTGGACAAAGCATCGGATAGAACGAAATTAGATCTTGAAAAGGAGCTTATTCAGTTTGTTGAGCTTCATCATTCACTCAATAAGGATACATGTTTCTCCAGCAAGAGTACTGCAGTAAGAAAAGGGAGTACTCGCACTCCCGCTCACGATATACTGAACAACACAAATTCAAGCCACTCTAAAGAAATGCAAGGACGGATTTCCTTCTTTGCAACTTCAAGCATATACCAGATATTGCAAACACTGTTGAGACTACTTGATACTGATTATTCTAATAATGTTGAAGCTTCCCAAATGCATAGTCAGTCTACCAGCAAGACATCCAAATGCAGCTCTGAATTTATTTCTTTTGTCTTGAATGTGTCCCTTAGTCATATAAAATCTTCCCTTCTTCTGCGAAATGAAGACCCTTTGAGAACTTTAATCTATGGGGAAATCAAGATCCTGGGGTCCCCCTTGTTGAAACTATTTTCTTTGTTCAAATCAGGGCCAAAGTTGGTGATGGATCAAAAGCAAAAGGAAGCGAAGGCGAAAAAAGATGTCGGACAAAGGGAGCATCTTCATCTGGCCTTGATTTGCTTGAAGGAATTGATAATGATTTGTTCTAATTCAGACTTGACTGGTTTGCTTGAAGACATGGTGTCAATTTCCACTCTTCAGGATGCTGATTTGGAAACTGAATGCGAAAGAGCCTCTAGAATTGAGGATCCACATATAAGGAGCAAAGAACTGTTCATTTTGAAGACATTGAAGCCTCTATTTTCTGAGCTTCTTGCAGTTTCATTATTTGGTGAAGTTGAGATTATTTGTGACCTGATATTGATGATTGGGGGAAAATTGCCATGCCAATTGAGGAACTCCCATGGAGCTTGGGCTATTAGTCTTTGCAAAAACAATGAAATAAAAAACACAAAGGTTGGAAAAAGTGTTGTGACACTTGCTATAAGTTTAAGCTCCCCTCCAAATGATCTAGCTATATCTCAAGACATGGCTAGGGAGTTGTTACAGGTTATTGGATCAGAGGGAGATACTCCAGAGGAGGTGTCTGAGATATACCCCCTCATAAACCATCAAACAAGTTCGGCAATAAATTCCTGTATACTGCACATTGTTGAAGCAGTTGTAGTTGACATGGATTGGGCCATCAAAAGATTGAAGACATTCTCTTTAGTCTCCCAAAAAGGGATTCATCTGAATCAGAACAGTGAGGAATCTCCTGAGTTGGCATTTGAGGAAAATCTCTATGCTAGGGCAGAAGCAGTGGTGAAAGTACTCTCATCTTTTGTTTCAATGAGCCTGAATGATACACAAGCTGAGCATGTGCTGCGACTGGCAGCAAGGTTTTACAAGCATTTAGCTCAGATGTCAAAGCTTAGGATCGCTACAAAAGGTTGTAAACAGATTTTTCCGAGCCTCAAGTTTCAGAAGCTTGTGGAGGTAACTTGCAAGGAGCTGACAGTTCCTCTGTATATCTTCGTGGAAATTATGCAAAAGAAACAACAAGAGAATGCCAATAAAAAGGGAATTATCAACAAAATAAAGAGGGAGAACAGATGCATCCCCGACCTCATATTTCAGATAGAAGATTATGAGAAATATCTCATCCAGCTTAGCAAGGTGACCAAAGTAAATTTATTAAGGAATGCCAAGCGTAGCACTGCACGAGACTTCAGAATCAAACTAAATGAGGAGAGCAGAAAAGAAGAAAATGCTCCGAGTCTTGAAGCAAACCATGACAATATGAATGCAGTTGAAAATGAGTCTGCTGAGGATCTTGATGATAATGAAGGAAATGCGTCAGATAGGGTTTTGTCACCGGATTATCTTAATAGTCCTCCTGCTGTGGAGGATTCAGGCTCTGACAATGAAGATGGAGATGATCATCCTCCTAGTGCCAAGAGGCTGAAGAGAGTCAACAGAGTTGTGGAGGACTCTGAGGATGAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
FANCI_HD1 FANCI_HD2 FANCI_S1 FANCI_S2 FANCI_S4

Protein Analysis

1405

Amino Acids

157.34

Weight (kDa)

6.19

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FANCI_S1 PF14675 113 - 278 2e-18 FANCI solenoid 1
FANCI_HD1 PF14679 282 - 363 5.4e-22 FANCI helical domain 1
FANCI_S2 PF14676 376 - 530 2.9e-49 FANCI solenoid 2
FANCI_HD2 PF14680 549 - 786 4.5e-55 FANCI helical domain 2
FANCI_S4 PF14678 1084 - 1318 8.7e-73 FANCI solenoid 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 685, 1273, 1820
AccBSI CCGCTC 3 cut(s) 138, 1094, 2411
AccI GTMKAC 4 cut(s) 618, 675, 2576, 3367
AccII CGCG 1 cut(s) 486
AciI CCGC 9 cut(s) 52, 136, 486, 489, 827, 1094, 1172, 2214, 2409
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 7 cut(s) 1304, 2020, 2077, 2434, 2606, 3358, 3898
AcuI CTGAAG 3 cut(s) 2909, 3922, 4193
AcyI GRCGYC 1 cut(s) 695
AdeI CACNNNGTG 1 cut(s) 1129
AfaI GTAC 7 cut(s) 348, 802, 1385, 2244, 2377, 2398, 3540
AfiI CCNNNNNNNGG 7 cut(s) 685, 847, 848, 1273, 1414, 1820, 3450
AflIII ACRYGT 2 cut(s) 1399, 2357
AhdI GACNNNNNGTC 2 cut(s) 664, 2908
AjnI CCWGG 1 cut(s) 2712
AjuI GAANNNNNNNTTGG 4 cut(s) 2139, 2171, 3438, 3470
Alw21I GWGCWC 5 cut(s) 513, 531, 779, 1197, 1725
Alw26I GTCTC 5 cut(s) 172, 2130, 2512, 3445, 3927
AlwNI CAGNNNCTG 2 cut(s) 523, 4127
AoxI GGCC 8 cut(s) 490, 603, 1080, 1263, 1708, 2756, 2835, 3408
ApeKI GCWGC 8 cut(s) 811, 1457, 1574, 1902, 1905, 2598, 3592, 3602
ApoI RAATTY 7 cut(s) 1304, 2020, 2077, 2434, 2606, 3358, 3898
ArsI GACNNNNNNTTYG 4 cut(s) 611, 643, 2554, 2586
Asp700I GAANNNNTTC 2 cut(s) 2077, 3428
AspLEI GCGC 2 cut(s) 8, 71
AspS9I GGNCC 8 cut(s) 537, 603, 838, 1264, 1810, 2718, 2756, 3408
AsuC2I CCSGG 2 cut(s) 445, 1408
AsuII TTCGAA 1 cut(s) 463
AvaII GGWCC 4 cut(s) 537, 838, 1810, 2718
BamHI GGATCC 2 cut(s) 1311, 2974
BanII GRGCYC 5 cut(s) 531, 779, 1197, 1725, 1947
BauI CACGAG 2 cut(s) 2246, 3930
BbsI GAAGAC 5 cut(s) 2199, 2679, 2907, 3017, 3431
Bbv12I GWGCWC 5 cut(s) 513, 531, 779, 1197, 1725
BbvCI CCTCAGC 1 cut(s) 4035
BbvI GCAGC 8 cut(s) 798, 1444, 1586, 1889, 1892, 2610, 3579, 3614
BceAI ACGGC 2 cut(s) 649, 1485
BciT130I CCWGG 1 cut(s) 2714
BciVI GTATCC 2 cut(s) 1020, 2347
BclI TGATCA 2 cut(s) 552, 4147
BcnI CCSGG 2 cut(s) 445, 1408
BcoDI GTCTC 5 cut(s) 172, 2130, 2512, 3445, 3927
BfmI CTRYAG 3 cut(s) 780, 2098, 2379
BfuI GTATCC 2 cut(s) 1020, 2347
BglII AGATCT 1 cut(s) 2299
BlpI GCTNAGC 3 cut(s) 55, 3582, 3878
BmcAI AGTACT 4 cut(s) 2244, 2377, 2398, 3540
Bme1390I CCNGG 3 cut(s) 445, 1408, 2714
Bme18I GGWCC 4 cut(s) 537, 838, 1810, 2718
BmeRI GACNNNNNGTC 2 cut(s) 664, 2908
BmgT120I GGNCC 8 cut(s) 537, 603, 838, 1264, 1810, 2718, 2756, 3408
BmiI GGNNCC 6 cut(s) 840, 1313, 1812, 2719, 2720, 2976
BmrFI CCNGG 3 cut(s) 445, 1408, 2714
BmrI ACTGGG 1 cut(s) 55
BmsI GCATC 9 cut(s) 962, 1233, 1867, 2160, 2288, 2833, 2920, 3812, 3834
BmuI ACTGGG 1 cut(s) 55
BpiI GAAGAC 5 cut(s) 2199, 2679, 2907, 3017, 3431
BpmI CTGGAG 3 cut(s) 545, 2350, 3285
Bpu10I CCTNAGC 2 cut(s) 3641, 4035
Bpu1102I GCTNAGC 3 cut(s) 55, 3582, 3878
Bpu14I TTCGAA 1 cut(s) 463
BpuEI CTTGAG 4 cut(s) 293, 1493, 3234, 3668
BpuMI CCSGG 2 cut(s) 445, 1408
BsaAI YACGTR 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 4149
BsaHI GRCGYC 1 cut(s) 695
BsaJI CCNNGG 3 cut(s) 443, 2713, 3130
BsaWI WCCGGW 1 cut(s) 4084
BsaXI ACNNNNNCTCC 8 cut(s) 171, 201, 287, 317, 931, 961, 2389, 2419
Bsc4I CCNNNNNNNGG 7 cut(s) 685, 847, 848, 1273, 1414, 1820, 3450
Bse118I RCCGGY 1 cut(s) 1756
Bse1I ACTGG 5 cut(s) 61, 164, 1665, 2893, 3603
Bse8I GATNNNNATC 1 cut(s) 4149
BseBI CCWGG 1 cut(s) 2714
BseDI CCNNGG 3 cut(s) 443, 2713, 3130
BseJI GATNNNNATC 1 cut(s) 4149
BseLI CCNNNNNNNGG 7 cut(s) 685, 847, 848, 1273, 1414, 1820, 3450
BseNI ACTGG 5 cut(s) 61, 164, 1665, 2893, 3603
BseXI GCAGC 8 cut(s) 798, 1444, 1586, 1889, 1892, 2610, 3579, 3614
BsgI GTGCAG 3 cut(s) 1040, 3356, 3912
Bsh1236I CGCG 1 cut(s) 486
Bsh1285I CGRYCG 1 cut(s) 570
BshFI GGCC 8 cut(s) 492, 605, 1082, 1265, 1710, 2758, 2837, 3410
BsiEI CGRYCG 1 cut(s) 570
BsiHKAI GWGCWC 5 cut(s) 513, 531, 779, 1197, 1725
BsiSI CCGG 4 cut(s) 445, 1408, 1757, 4085
BslFI GGGAC 4 cut(s) 590, 864, 2617, 2704
BslI CCNNNNNNNGG 7 cut(s) 685, 847, 848, 1273, 1414, 1820, 3450
BsmAI GTCTC 5 cut(s) 172, 2130, 2512, 3445, 3927
BsmFI GGGAC 4 cut(s) 590, 864, 2617, 2704
BsmI GAATGC 5 cut(s) 2257, 2954, 3781, 3916, 4018
BsnI GGCC 8 cut(s) 492, 605, 1082, 1265, 1710, 2758, 2837, 3410
Bsp119I TTCGAA 1 cut(s) 463
Bsp1286I GDGCHC 6 cut(s) 513, 531, 779, 1197, 1725, 1947
Bsp1720I GCTNAGC 3 cut(s) 55, 3582, 3878
Bsp19I CCATGG 1 cut(s) 3130
BspACI CCGC 9 cut(s) 52, 136, 486, 489, 827, 1094, 1172, 2214, 2409
BspANI GGCC 8 cut(s) 492, 605, 1082, 1265, 1710, 2758, 2837, 3410
BspFNI CGCG 1 cut(s) 486
BspLI GGNNCC 6 cut(s) 840, 1313, 1812, 2719, 2720, 2976
BspMAI CTGCAG 1 cut(s) 2383
BspQI GCTCTTC 2 cut(s) 1935, 2185
BspT104I TTCGAA 1 cut(s) 463
BsrBI CCGCTC 3 cut(s) 138, 1094, 2411
BsrFI RCCGGY 1 cut(s) 1756
BsrI ACTGG 5 cut(s) 61, 164, 1665, 2893, 3603
BssAI RCCGGY 1 cut(s) 1756
BssECI CCNNGG 3 cut(s) 443, 2713, 3130
BssNAI GTATAC 1 cut(s) 3368
BssNI GRCGYC 1 cut(s) 695
BssSI CACGAG 2 cut(s) 2246, 3930
BssT1I CCWWGG 1 cut(s) 3130
Bst1107I GTATAC 1 cut(s) 3368
Bst2BI CACGAG 2 cut(s) 2246, 3930
Bst2UI CCWGG 1 cut(s) 2714
Bst6I CTCTTC 6 cut(s) 275, 1170, 1935, 2185, 2928, 4169
BstACI GRCGYC 1 cut(s) 695
BstAPI GCANNNNNTGC 2 cut(s) 1583, 3592
BstBAI YACGTR 1 cut(s) 346
BstBI TTCGAA 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 775, 1233
BstDSI CCRYGG 1 cut(s) 3130
BstEII GGTNACC 1 cut(s) 3886
BstFNI CGCG 1 cut(s) 486
BstHHI GCGC 2 cut(s) 8, 71
BstMAI GTCTC 5 cut(s) 172, 2130, 2512, 3445, 3927
BstMCI CGRYCG 1 cut(s) 570
BstMWI GCNNNNNNNGC 2 cut(s) 1583, 3592
BstNI CCWGG 1 cut(s) 2714
BstNSI RCATGY 5 cut(s) 818, 1403, 2037, 2361, 3590
BstPI GGTNACC 1 cut(s) 3886
BstSCI CCNGG 3 cut(s) 443, 1406, 2712
BstSFI CTRYAG 3 cut(s) 780, 2098, 2379
BstUI CGCG 1 cut(s) 486
BstV1I GCAGC 8 cut(s) 798, 1444, 1586, 1889, 1892, 2610, 3579, 3614
BstV2I GAAGAC 5 cut(s) 2199, 2679, 2907, 3017, 3431
BstX2I RGATCY 6 cut(s) 426, 1311, 2299, 2709, 2974, 4039
BstYI RGATCY 6 cut(s) 426, 1311, 2299, 2709, 2974, 4039
BstZ17I GTATAC 1 cut(s) 3368
BsuI GTATCC 2 cut(s) 1020, 2347
BsuRI GGCC 8 cut(s) 492, 605, 1082, 1265, 1710, 2758, 2837, 3410
BtgI CCRYGG 1 cut(s) 3130
BtgZI GCGATG 1 cut(s) 1556
BtsI GCAGTG 3 cut(s) 1304, 3534, 3924
BtsIMutI CAGTG 6 cut(s) 1304, 1732, 2510, 3478, 3534, 3924
Cac8I GCNNGC 2 cut(s) 775, 1233
CaiI CAGNNNCTG 2 cut(s) 523, 4127
CfoI GCGC 2 cut(s) 8, 71
Cfr10I RCCGGY 1 cut(s) 1756
Cfr13I GGNCC 8 cut(s) 537, 603, 838, 1264, 1810, 2718, 2756, 3408
CseI GACGC 2 cut(s) 684, 4053
Csp6I GTAC 7 cut(s) 347, 801, 1384, 2243, 2376, 2397, 3539
CspCI CAANNNNNGTGG 2 cut(s) 1189, 1224
CviQI GTAC 7 cut(s) 347, 801, 1384, 2243, 2376, 2397, 3539
DraI TTTAAA 1 cut(s) 1870
DraIII CACNNNGTG 1 cut(s) 1129
DriI GACNNNNNGTC 2 cut(s) 664, 2908
EaeI YGGCCR 1 cut(s) 490
Eam1104I CTCTTC 6 cut(s) 275, 1170, 1935, 2185, 2928, 4169
Eam1105I GACNNNNNGTC 2 cut(s) 664, 2908
EarI CTCTTC 6 cut(s) 275, 1170, 1935, 2185, 2928, 4169
Ecl136II GAGCTC 4 cut(s) 529, 777, 1195, 1723
Eco130I CCWWGG 1 cut(s) 3130
Eco147I AGGCCT 1 cut(s) 1710
Eco24I GRGCYC 5 cut(s) 531, 779, 1197, 1725, 1947
Eco32I GATATC 1 cut(s) 46
Eco47I GGWCC 4 cut(s) 537, 838, 1810, 2718
Eco53kI GAGCTC 4 cut(s) 529, 777, 1195, 1723
Eco57I CTGAAG 3 cut(s) 2909, 3922, 4193
Eco91I GGTNACC 1 cut(s) 3886
EcoICRI GAGCTC 4 cut(s) 529, 777, 1195, 1723
EcoO109I RGGNCCY 2 cut(s) 838, 2718
EcoO65I GGTNACC 1 cut(s) 3886
EcoRI GAATTC 1 cut(s) 2020
EcoRII CCWGG 1 cut(s) 2712
EcoRV GATATC 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 3130
EcoT22I ATGCAT 3 cut(s) 1446, 2568, 3827
EcoT38I GRGCYC 5 cut(s) 531, 779, 1197, 1725, 1947
ErhI CCWWGG 1 cut(s) 3130
FaqI GGGAC 4 cut(s) 590, 864, 2617, 2704
FauI CCCGC 2 cut(s) 143, 2416
FbaI TGATCA 2 cut(s) 552, 4147
FblI GTMKAC 4 cut(s) 618, 675, 2576, 3367
FriOI GRGCYC 5 cut(s) 531, 779, 1197, 1725, 1947
GlaI GCGC 2 cut(s) 7, 70
GsuI CTGGAG 3 cut(s) 545, 2350, 3285
HaeIII GGCC 8 cut(s) 492, 605, 1082, 1265, 1710, 2758, 2837, 3410
HapII CCGG 4 cut(s) 445, 1408, 1757, 4085
HgaI GACGC 2 cut(s) 684, 4053
HhaI GCGC 2 cut(s) 8, 71
Hin1I GRCGYC 1 cut(s) 695
Hin6I GCGC 2 cut(s) 6, 69
HinP1I GCGC 2 cut(s) 6, 69
HincII GTYRAC 4 cut(s) 820, 2089, 3397, 4183
HindII GTYRAC 4 cut(s) 820, 2089, 3397, 4183
HindIII AAGCTT 5 cut(s) 1796, 1929, 2553, 3638, 3695
HpaII CCGG 4 cut(s) 445, 1408, 1757, 4085
Hpy166II GTNNAC 9 cut(s) 619, 676, 820, 2089, 2577, 3368, 3397, 3665, 4183
Hpy8I GTNNAC 9 cut(s) 619, 676, 820, 2089, 2577, 3368, 3397, 3665, 4183
Hpy99I CGWCG 3 cut(s) 476, 670, 700
HpyCH4IV ACGT 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 1583, 3592
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 695
HspAI GCGC 2 cut(s) 6, 69
KflI GGGWCCC 1 cut(s) 2718
Ksp22I TGATCA 2 cut(s) 552, 4147
LguI GCTCTTC 2 cut(s) 1935, 2185
Lsp1109I GCAGC 8 cut(s) 798, 1444, 1586, 1889, 1892, 2610, 3579, 3614
LweI GCATC 9 cut(s) 962, 1233, 1867, 2160, 2288, 2833, 2920, 3812, 3834
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 8 cut(s) 1468, 1980, 3077, 3199, 3270, 3706, 3886, 4080
MbiI CCGCTC 3 cut(s) 138, 1094, 2411
MfeI CAATTG 3 cut(s) 532, 680, 3116
MflI RGATCY 6 cut(s) 426, 1311, 2299, 2709, 2974, 4039
MhlI GDGCHC 6 cut(s) 513, 531, 779, 1197, 1725, 1947
MlyI GAGTC 9 cut(s) 32, 404, 1175, 1658, 2084, 3994, 4037, 4188, 4193
MmeI TCCRAC 6 cut(s) 195, 495, 1404, 2250, 2791, 3166
Mph1103I ATGCAT 3 cut(s) 1446, 2568, 3827
MroXI GAANNNNTTC 2 cut(s) 2077, 3428
MseI TTAA 9 cut(s) 711, 1088, 1098, 1205, 1869, 2690, 3218, 3905, 4095
MslI CAYNNNNRTG 3 cut(s) 1439, 2032, 2593
MspA1I CMGCKG 1 cut(s) 1172
MspI CCGG 4 cut(s) 445, 1408, 1757, 4085
MspR9I CCNGG 3 cut(s) 445, 1408, 2714
MunI CAATTG 3 cut(s) 532, 680, 3116
Mva1269I GAATGC 5 cut(s) 2257, 2954, 3781, 3916, 4018
MvaI CCWGG 1 cut(s) 2714
MvnI CGCG 1 cut(s) 486
MwoI GCNNNNNNNGC 2 cut(s) 1583, 3592
NciI CCSGG 2 cut(s) 445, 1408
NcoI CCATGG 1 cut(s) 3130
NlaIV GGNNCC 6 cut(s) 840, 1313, 1812, 2719, 2720, 2976
NmuCI GTSAC 4 cut(s) 3077, 3199, 3886, 4080
NsiI ATGCAT 3 cut(s) 1446, 2568, 3827
NspI RCATGY 5 cut(s) 818, 1403, 2037, 2361, 3590
NspV TTCGAA 1 cut(s) 463
PceI AGGCCT 1 cut(s) 1710
PciI ACATGT 2 cut(s) 1399, 2357
PciSI GCTCTTC 2 cut(s) 1935, 2185
PcsI WCGNNNNNNNCGW 1 cut(s) 2288
PctI GAATGC 5 cut(s) 2257, 2954, 3781, 3916, 4018
PdmI GAANNNNTTC 2 cut(s) 2077, 3428
PflFI GACNNNGTC 1 cut(s) 476
PflMI CCANNNNNTGG 3 cut(s) 685, 1273, 1820
Ple19I CGATCG 1 cut(s) 570
PleI GAGTC 9 cut(s) 32, 404, 1174, 1657, 2084, 3993, 4036, 4187, 4193
PpsI GAGTC 9 cut(s) 32, 404, 1174, 1657, 2084, 3993, 4036, 4187, 4193
Ppu21I YACGTR 1 cut(s) 346
PpuMI RGGWCCY 2 cut(s) 838, 2718
PscI ACATGT 2 cut(s) 1399, 2357
Psp124BI GAGCTC 4 cut(s) 531, 779, 1197, 1725
Psp5II RGGWCCY 2 cut(s) 838, 2718
Psp6I CCWGG 1 cut(s) 2712
PspEI GGTNACC 1 cut(s) 3886
PspGI CCWGG 1 cut(s) 2712
PspN4I GGNNCC 6 cut(s) 840, 1313, 1812, 2719, 2720, 2976
PspPI GGNCC 8 cut(s) 537, 603, 838, 1264, 1810, 2718, 2756, 3408
PspPPI RGGWCCY 2 cut(s) 838, 2718
PstI CTGCAG 1 cut(s) 2383
PstNI CAGNNNCTG 2 cut(s) 523, 4127
PsuI RGATCY 6 cut(s) 426, 1311, 2299, 2709, 2974, 4039
PsyI GACNNNGTC 1 cut(s) 476
PvuI CGATCG 1 cut(s) 570
RsaI GTAC 7 cut(s) 348, 802, 1385, 2244, 2377, 2398, 3540
RsaNI GTAC 7 cut(s) 347, 801, 1384, 2243, 2376, 2397, 3539
RseI CAYNNNNRTG 3 cut(s) 1439, 2032, 2593
SacI GAGCTC 4 cut(s) 531, 779, 1197, 1725
SapI GCTCTTC 2 cut(s) 1935, 2185
SaqAI TTAA 9 cut(s) 711, 1088, 1098, 1205, 1869, 2690, 3218, 3905, 4095
Sau96I GGNCC 8 cut(s) 537, 603, 838, 1264, 1810, 2718, 2756, 3408
ScaI AGTACT 4 cut(s) 2244, 2377, 2398, 3540
SchI GAGTC 9 cut(s) 32, 404, 1175, 1658, 2084, 3994, 4037, 4188, 4193
ScrFI CCNGG 3 cut(s) 445, 1408, 2714
SduI GDGCHC 6 cut(s) 513, 531, 779, 1197, 1725, 1947
SfaNI GCATC 9 cut(s) 962, 1233, 1867, 2160, 2288, 2833, 2920, 3812, 3834
SfcI CTRYAG 3 cut(s) 780, 2098, 2379
SfuI TTCGAA 1 cut(s) 463
SinI GGWCC 4 cut(s) 537, 838, 1810, 2718
SmiMI CAYNNNNRTG 3 cut(s) 1439, 2032, 2593
SmlI CTYRAG 4 cut(s) 308, 1508, 3249, 3683
SmoI CTYRAG 4 cut(s) 308, 1508, 3249, 3683
SseBI AGGCCT 1 cut(s) 1710
SsiI CCGC 9 cut(s) 52, 136, 486, 489, 827, 1094, 1172, 2214, 2409
SspI AATATT 1 cut(s) 2143
SstI GAGCTC 4 cut(s) 531, 779, 1197, 1725
StuI AGGCCT 1 cut(s) 1710
StyD4I CCNGG 3 cut(s) 443, 1406, 2712
StyI CCWWGG 1 cut(s) 3130
TaiI ACGT 1 cut(s) 348
TatI WGTACW 4 cut(s) 2242, 2375, 2396, 3538
TauI GCSGC 2 cut(s) 489, 492
Tru1I TTAA 9 cut(s) 711, 1088, 1098, 1205, 1869, 2690, 3218, 3905, 4095
Tru9I TTAA 9 cut(s) 711, 1088, 1098, 1205, 1869, 2690, 3218, 3905, 4095
TscAI CASTG 6 cut(s) 1304, 1732, 2517, 3478, 3534, 3931
TseFI GTSAC 4 cut(s) 3077, 3199, 3886, 4080
TseI GCWGC 8 cut(s) 811, 1457, 1574, 1902, 1905, 2598, 3592, 3602
Tsp45I GTSAC 4 cut(s) 3077, 3199, 3886, 4080
TspGWI ACGGA 2 cut(s) 1371, 2479
TspRI CASTG 6 cut(s) 1304, 1732, 2517, 3478, 3534, 3931
Tth111I GACNNNGTC 1 cut(s) 476
Van91I CCANNNNNTGG 3 cut(s) 685, 1273, 1820
VpaK11BI GGWCC 4 cut(s) 537, 838, 1810, 2718
XapI RAATTY 7 cut(s) 1304, 2020, 2077, 2434, 2606, 3358, 3898
XbaI TCTAGA 1 cut(s) 2963
XceI RCATGY 5 cut(s) 818, 1403, 2037, 2361, 3590
XmiI GTMKAC 4 cut(s) 618, 675, 2576, 3367
XmnI GAANNNNTTC 2 cut(s) 2077, 3428
ZrmI AGTACT 4 cut(s) 2244, 2377, 2398, 3540
Zsp2I ATGCAT 3 cut(s) 1446, 2568, 3827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.